Rh4AG171600
ERF Family

Probably involved in the RNA silencing pathway and required for the generation of small interfering RNAs (siRNAs)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0008702
Physical Location & Seq
Forward (+)
43144521 .. 43149603
5083 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG171600.1

Sequence Viewer

Length: 3594 bp
ATGGAATTAGAAGGAAGGGAAAATGACACAGTGGTAACTCAAGTCAGCTTTGGTGGATTTGGAAGTCATGTTAAAGCAAAAGATCTTGTAGACTACTTGGAAGATGAAATTGGGCTTGTATATAGGTGTCGGTTGAAAACTTCTTGGACCCCTCCAGAGTCTCTTCCGAGCTTTGAGCTCGTTGATACTGCAGATATTGAAACAACAGATGAATATAAAAAGGTTGAGCCACATGCATTTGTGCATTTTGCTTCGCCCGAGTCAGTGACTGCTGCTATGGATGCTGTTGGGCGCTGTCAACTCTTTCTCAAGAACCAAGCACTGAAAGTAAGTTTGGGCCCTGAGAATTCATACAACATGAACAAGAGGAGGAGGACTAAAATTCCATTCAAGTTAGCTGATGTGCGTCTTGAGATTGGAAGTTTAGTTCGCCTAGATGAGTTCTTTGTTGCTTGGAGGGGGCCATCTTATGGGATTGATTTTGTTGTGGATCCTTTTGATGGGACATGCAAATTTTGTTTTAGAAGAGATACTGCCTTTTCATTTAAAGGGATCAATAAGTATGCTGTGATAAAATGCGATTTTAAGGTGGAGTTCCTGGTAAGAGAAATCAGTGAATTTAAACACTATACAGAGCCTTCATATCTTGTAATTCTGTTGAGGCTGGCTTCATCACCTTGGGTTTCATATCGAACTGCCGATGATGATATTGATCAATCAGTTCCATTTGATTTGTTGGATGACGATGATCCTTGGATTAGAACCACAGATTTTACTCCTAGTGGGGCAATCGGGCGTTGTAATTCTTACAGAGTTTTAATCCCACCGCGGCATGGTGCTAAGCTGAAGAAGGCCATGAGTTATCTTAAAGAACGCAGGGTGCAAGAGGTTTCGATCAAAAAGCCACCTAGGATTCAGAATGAACCTGATTTTGAAATGCCCATGTCAGATCCTTTCTTCTCTATTCATTACAAGGAAAATATTTCCTTTGAGATATTGTTCTTGGTCAATGCTGTAATGCACAGGGGCATTTTAAATCAGCACCAGTTGTCAGACGGCTTTTTTGATTTACTGAGAAACCAACCAAAGGAGATCAATGTGGCTGCATTGAAGCACTTATGTTCATACAAACACCCGGTTTTTGATGCCTCTAAAAGGCTGAAACTTGTCCAAGAATGGTTGCTGAGAAACCCCAAGCTTATCAAGAATCCCAAACATTTGGATGATATTGCTGAAGTTAGGAGGTTGGTGATTACCCCAACTAAAGCCTATTGTCTTCCACCAGAGGTTGAACTATCCAACAGGGTTCTTAGAAAATATAAAGAAGTTGCTGATAGGTTTTTAAGGGTTACCTTTATGGATGAAGGAATGCAGACAATAAATTCCAATGTTATGAATTCTTATGTTGCTCCTATTGTGAAGGAAATCACAGAAAACACCATCCCTCAGAAAACAAAAGTGTATGCAAGGGTTCGGGCCATTCTGATGAAGGGATTTCAATTATGTGGTCGTAAGTACTCGTTCTTGGCCTTCTCATCCAATCAACTGAGGGACCGTTCTGCCTGGTTTTTCGCTGAAGACAAGAAGATTAGTATACAAGGAATCAAAAATTGGATGGGGAAGTTCCACAACAGAAACGTTGCAAAATGTGCTGCTAGGATGGGTCAGTGTTTTTCGTCTACATATGCAACGGTAGAAGTTCCATCAGCCCAGGTTAATCCTAAACTTCCAGATATTGAGAGAAATGACTATATCTTTTCTGATGGTATTGGTAGGATCACCCCAGACCTTGCAATGGAAGTTGCAGAAAGACTGAAATTGGACAGAGATCCACCTTGTGCATATCAAATTAGATACGGAGGCTGTAAAGGGGTTATTGCTTGTTGGCCACCGACAGGTGATGGCTTCCGGCTTTCTTTGAGGCCTAGTATGAACAAGTTTGAGTCGGACCATACTATATTGGAAATATGTTCATGGACTAGGTTCCAGCCAGGTTTCTTAAACAGGCAGATTATAACATTGCTTTCAGCACTTGGTGTTCCAGATGAAATATTCTGGACAATGCAGGAGACAATGGTTTCCAAATTAAAGCAGATGCTGGTGGACACAGATGTGGCGTTTGATGTTCTCACTGCATCCTGTGCTGAGCAAGGAAATGCTGCAGCTATAATGTTAAGTGCAGGTTTCAAGCCCCAGACAGAACCACATCTAAGAGGCATGTTGACCTGTATACAAGCCGCACAGCTCTGGGGACTTAGGGAAAAGGCCAGGATTTTTGTTCCTTCTGGGAGATGGCTTATGGGCTGTCTGGATGAGTTAGGGGTACTTGAGCAAGGCCAGTGCTTTGTCCAAGTGTCTACCCCATCGCTGGAAAACTGTTTTGCAAAGCATGGCTCTAGGTTTGATCAGATAGATAAAAATCTACAAGTAATAAAAGGGTTCGTAGTGGTAGCAAAGAATCCTTGCCTTCATCCAGGAGATATAAGGATTCTTGAAGCAGTTGATGCACCTGGGTTACACCACTTGCATGATTGCCTTGTTTTCCCTCAGAAAGGTCATAGGCCTCACACAGATGAAGCTTCTGGTAGTGACCTAGATGGGGACCTCTACTTTGTCACATGGGATGAAAATCTTATTCCCCCACGTAAGAAGAGCTGGGAACCTATGCAGTATGAAGCTGCAGAAGCCAAAGTTTCAGGGCGTCCTGTCACGAACCAGGATTTAATCGAGTTTTTTGTGAGAAACATGGTGAATGAGAACCTGGGAGCGATATGCAATGCACATGTGGTTCATTCTGACCGCAGTGAATATGGAGCTTTCGATGAGAAGTGCCTAAAACTAGCAGAATTAGCAGCCATAGCAGTTGATTTTCCCAAAACTGGGAAGATTGTTAACATGCCACCACCCCTAAAGCCAAAGCTATACCCAGATTTCATGGGAAAAGAGGACTACCAGTCGTACAAGTCTACTAAAATTTTAGGGAGACTCTATCGCAAGATTTGCGATGCTTATGATGAAGATGTGGCAACCTCTTCTGAGCAAAATCACAATCCCAGTGACATCAAATATGATATGGATCTGGAGGTTTCAAAAGCTGCAGATTTCATAGCTGATGCTTGGGAGAGAAAGTGCTCTTATGATGATCAGCTACAGGGTCTAATGGCACAGTATAAAGTTATGAGGGAAGAGGAGATTGTCACAGGGCATGTTTGGTCAATGCCCAAGTATACCAGCAGGAAGCAAGGGGAGCTGCAGGAGCGGTTGAGGCATTCTTACAGTGCTCTGAAGAAAGAGTTCAGGCAATTTTTCGAGAAGATGGATACGGGTTTTGAGTCACTGACTGATGATCAGAAAAATAGCTTGTATGAGCAGAAAGCCTCAGCTTGGTACCAGGTGACTTACCACCCTGATTGGGTGAAAAGGTCACTCAATTTTCAAAGGCCAGATGGCCCAGGAGATGTAGTGATGTTAAGCTTTGCCTGGATTGCAGCTGACTACCTTGCTCGGATCAAGATCAAGCGCCGTGAAGTGGGTAATATTGACTCTAAGAAGCCAATCAATGCTTTGACGAAGTACCTTGCTGATCGGATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001172 GO:0002252 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0003968 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007275 GO:0008037 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009605 GO:0009607 GO:0009615 GO:0009616 GO:0009791 GO:0009856 GO:0009875 GO:0009888 GO:0009892 GO:0009908 GO:0009987 GO:0010033 GO:0010073 GO:0010074 GO:0010267 GO:0010467 GO:0010468 GO:0010492 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019222 GO:0019438 GO:0019827 GO:0022414 GO:0030422 GO:0031047 GO:0031050 GO:0032501 GO:0032502 GO:0032774 GO:0034062 GO:0034641 GO:0034654 GO:0035194 GO:0035821 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043331 GO:0044003 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044403 GO:0044419 GO:0044424 GO:0044464 GO:0044706 GO:0045087 GO:0046483 GO:0048366 GO:0048367 GO:0048437 GO:0048438 GO:0048440 GO:0048467 GO:0048507 GO:0048519 GO:0048544 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050896 GO:0051607 GO:0051701 GO:0051704 GO:0051707 GO:0051716 GO:0051817 GO:0052018 GO:0052249 GO:0060255 GO:0061458 GO:0065007 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0090567 GO:0097659 GO:0097747 GO:0098542 GO:0098586 GO:0098727 GO:0099402 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1901698 GO:1901699
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RdRP

Protein Analysis

1197

Amino Acids

136.97

Weight (kDa)

7.67

Isoelectric Point (pI)

33.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RBD_RDR6 PF24572 10 - 111 2.6e-33 RNA-dependent RNA polymerase 6, RNA-binding domain
RDR6_2nd PF24577 131 - 294 3.5e-84 RNA-dependent RNA polymerase 6 second domain
RdRP_helical PF26252 318 - 400 2.8e-23 RNA dependent RNA polymerase, helical domain
RdRP PF05183 418 - 1000 2.6e-195 RNA dependent RNA polymerase
RdRP_head PF26253 1023 - 1185 1.4e-44 RdRP, head domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2015
Acc36I ACCTGC 1 cut(s) 2171
Acc65I GGTACC 1 cut(s) 3387
AccB1I GGYRCC 1 cut(s) 3387
AccB7I CCANNNNNTGG 1 cut(s) 470
AccBSI CCGCTC 1 cut(s) 3259
AccI GTMKAC 7 cut(s) 90, 1594, 1679, 2230, 2357, 2966, 3227
AccII CGCG 1 cut(s) 829
AciI CCGC 5 cut(s) 827, 829, 2238, 2800, 3259
AclI AACGTT 1 cut(s) 1638
AclWI GGATC 9 cut(s) 485, 498, 560, 743, 944, 1784, 1823, 3084, 3515
AcoI YGGCCR 1 cut(s) 1886
AcsI RAATTY 7 cut(s) 346, 381, 512, 617, 1381, 1396, 2973
AcuI CTGAAG 4 cut(s) 866, 1254, 1596, 3305
AcyI GRCGYC 1 cut(s) 2701
AdeI CACNNNGTG 2 cut(s) 1838, 2036
AfaI GTAC 5 cut(s) 1517, 2325, 2960, 3389, 3575
AflIII ACRYGT 1 cut(s) 2782
AhdI GACNNNNNGTC 1 cut(s) 2953
AjuI GAANNNNNNNTTGG 6 cut(s) 93, 125, 317, 349, 1594, 1626
AleI CACNNNNGTG 1 cut(s) 2111
AloI GAACNNNNNNTCC 2 cut(s) 411, 443
Alw21I GWGCWC 3 cut(s) 180, 3134, 3283
Alw26I GTCTC 3 cut(s) 165, 2063, 2977
AlwI GGATC 9 cut(s) 485, 498, 560, 743, 944, 1784, 1823, 3084, 3515
AlwNI CAGNNNCTG 2 cut(s) 269, 2098
Ama87I CYCGRG 1 cut(s) 257
ApaI GGGCCC 1 cut(s) 341
ApoI RAATTY 7 cut(s) 346, 381, 512, 617, 1381, 1396, 2973
ArsI GACNNNNNNTTYG 4 cut(s) 1046, 1078, 2594, 2626
Asp700I GAANNNNTTC 1 cut(s) 3293
Asp718I GGTACC 1 cut(s) 3387
AspA2I CCTAGG 1 cut(s) 908
AspLEI GCGC 2 cut(s) 294, 3522
AspS9I GGNCC 9 cut(s) 147, 337, 338, 461, 1476, 1552, 1948, 2602, 3449
AsuC2I CCSGG 1 cut(s) 1136
AsuHPI GGTGA 7 cut(s) 666, 1261, 1771, 1910, 2761, 3406, 3427
AvaI CYCGRG 1 cut(s) 257
AvaII GGWCC 4 cut(s) 147, 1552, 1948, 2602
AvrII CCTAGG 1 cut(s) 908
BaeGI GKGCMC 1 cut(s) 341
BalI TGGCCA 1 cut(s) 1888
BamHI GGATCC 1 cut(s) 490
BanI GGYRCC 1 cut(s) 3387
BanII GRGCYC 2 cut(s) 180, 341
BbsI GAAGAC 2 cut(s) 1268, 1584
Bbv12I GWGCWC 3 cut(s) 180, 3134, 3283
BbvCI CCTCAGC 1 cut(s) 3379
BceAI ACGGC 2 cut(s) 1072, 3507
BciVI GTATCC 1 cut(s) 3313
BclI TGATCA 4 cut(s) 712, 2404, 3142, 3346
BcnI CCSGG 1 cut(s) 1136
BcoDI GTCTC 3 cut(s) 165, 2063, 2977
BfaI CTAG 9 cut(s) 434, 780, 909, 1656, 1926, 1980, 2397, 2594, 2840
BfmI CTRYAG 6 cut(s) 189, 2160, 2679, 3096, 3149, 3251
BfoI RGCGCY 2 cut(s) 295, 3523
BfuAI ACCTGC 1 cut(s) 2171
BfuI GTATCC 1 cut(s) 3313
BglII AGATCT 1 cut(s) 82
BlnI CCTAGG 1 cut(s) 908
BlpI GCTNAGC 2 cut(s) 840, 2145
BmcAI AGTACT 1 cut(s) 1517
Bme18I GGWCC 4 cut(s) 147, 1552, 1948, 2602
BmeRI GACNNNNNGTC 1 cut(s) 2953
BmeT110I CYCGRG 1 cut(s) 257
BmgT120I GGNCC 9 cut(s) 147, 337, 338, 461, 1476, 1552, 1948, 2602, 3449
BmiI GGNNCC 9 cut(s) 149, 339, 462, 492, 1553, 1985, 2603, 2661, 3389
BmrI ACTGGG 2 cut(s) 2889, 3048
BmsI GCATC 7 cut(s) 271, 1135, 2085, 2144, 2494, 2995, 3103
BmuI ACTGGG 2 cut(s) 2889, 3048
BpiI GAAGAC 2 cut(s) 1268, 1584
BpmI CTGGAG 2 cut(s) 138, 3101
Bpu10I CCTNAGC 1 cut(s) 3379
Bpu1102I GCTNAGC 2 cut(s) 840, 2145
BpuEI CTTGAG 4 cut(s) 24, 293, 431, 2348
BpuMI CCSGG 1 cut(s) 1136
BsaAI YACGTR 1 cut(s) 2645
BsaBI GATNNNNATC 5 cut(s) 711, 2418, 2628, 3075, 3512
BsaHI GRCGYC 1 cut(s) 2701
BsaJI CCNNGG 8 cut(s) 677, 752, 827, 908, 1710, 2510, 2761, 3451
BsaXI ACNNNNNCTCC 4 cut(s) 3113, 3143, 3447, 3477
Bse1I ACTGG 5 cut(s) 1045, 2338, 2884, 2953, 3054
Bse3DI GCAATG 3 cut(s) 1800, 2018, 2782
Bse8I GATNNNNATC 5 cut(s) 711, 2418, 2628, 3075, 3512
BseDI CCNNGG 8 cut(s) 677, 752, 827, 908, 1710, 2510, 2761, 3451
BseJI GATNNNNATC 5 cut(s) 711, 2418, 2628, 3075, 3512
BseMI GCAATG 3 cut(s) 1800, 2018, 2782
BseMII CTCAG 9 cut(s) 333, 1064, 1175, 1460, 1538, 2136, 2561, 3027, 3393
BseNI ACTGG 5 cut(s) 1045, 2338, 2884, 2953, 3054
BseRI GAGGAG 3 cut(s) 382, 385, 3203
BseSI GKGCMC 1 cut(s) 341
BseYI CCCAGC 1 cut(s) 2655
BsgI GTGCAG 1 cut(s) 2199
Bsh1236I CGCG 1 cut(s) 829
BshNI GGYRCC 1 cut(s) 3387
BsiHKAI GWGCWC 3 cut(s) 180, 3134, 3283
BsiHKCI CYCGRG 1 cut(s) 257
BsiSI CCGG 2 cut(s) 1136, 1909
BslFI GGGAC 4 cut(s) 517, 1565, 2265, 2615
BsmAI GTCTC 3 cut(s) 165, 2063, 2977
BsmFI GGGAC 4 cut(s) 517, 1565, 2265, 2615
BsmI GAATGC 2 cut(s) 1374, 3268
BsoBI CYCGRG 1 cut(s) 257
Bsp120I GGGCCC 1 cut(s) 337
Bsp1286I GDGCHC 4 cut(s) 180, 341, 3134, 3283
Bsp1720I GCTNAGC 2 cut(s) 840, 2145
BspACI CCGC 5 cut(s) 827, 829, 2238, 2800, 3259
BspCNI CTCAG 9 cut(s) 334, 1065, 1176, 1459, 1539, 2137, 2560, 3028, 3392
BspFNI CGCG 1 cut(s) 829
BspLI GGNNCC 9 cut(s) 149, 339, 462, 492, 1553, 1985, 2603, 2661, 3389
BspMAI CTGCAG 5 cut(s) 193, 2164, 2683, 3100, 3255
BspMI ACCTGC 1 cut(s) 2171
BspPI GGATC 9 cut(s) 485, 498, 560, 743, 944, 1784, 1823, 3084, 3515
BspQI GCTCTTC 1 cut(s) 2645
BspT107I GGYRCC 1 cut(s) 3387
BsrBI CCGCTC 1 cut(s) 3259
BsrDI GCAATG 3 cut(s) 1800, 2018, 2782
BsrI ACTGG 5 cut(s) 1045, 2338, 2884, 2953, 3054
BssECI CCNNGG 8 cut(s) 677, 752, 827, 908, 1710, 2510, 2761, 3451
BssNAI GTATAC 3 cut(s) 1595, 2231, 3228
BssNI GRCGYC 1 cut(s) 2701
BssT1I CCWWGG 3 cut(s) 677, 752, 908
Bst1107I GTATAC 3 cut(s) 1595, 2231, 3228
Bst4CI ACNGT 6 cut(s) 31, 1556, 1693, 2378, 3168, 3278
Bst6I CTCTTC 5 cut(s) 168, 520, 2645, 3037, 3180
BstACI GRCGYC 1 cut(s) 2701
BstAPI GCANNNNNTGC 4 cut(s) 1649, 2141, 2504, 3000
BstBAI YACGTR 1 cut(s) 2645
BstC8I GCNNGC 1 cut(s) 666
BstDSI CCRYGG 1 cut(s) 827
BstEII GGTNACC 1 cut(s) 1348
BstENI CCTNNNNNAGG 2 cut(s) 1153, 2550
BstFNI CGCG 1 cut(s) 829
BstH2I RGCGCY 2 cut(s) 295, 3523
BstHHI GCGC 2 cut(s) 294, 3522
BstMAI GTCTC 3 cut(s) 165, 2063, 2977
BstNSI RCATGY 6 cut(s) 236, 510, 2221, 2786, 2899, 3209
BstPI GGTNACC 1 cut(s) 1348
BstSFI CTRYAG 6 cut(s) 189, 2160, 2679, 3096, 3149, 3251
BstSLI GKGCMC 1 cut(s) 341
BstUI CGCG 1 cut(s) 829
BstV2I GAAGAC 2 cut(s) 1268, 1584
BstX2I RGATCY 5 cut(s) 82, 490, 949, 1828, 3076
BstXI CCANNNNNNTGG 1 cut(s) 1219
BstYI RGATCY 5 cut(s) 82, 490, 949, 1828, 3076
BstZ17I GTATAC 3 cut(s) 1595, 2231, 3228
BsuI GTATCC 1 cut(s) 3313
BtgI CCRYGG 1 cut(s) 827
BtgZI GCGATG 2 cut(s) 2349, 3018
BtsI GCAGTG 2 cut(s) 2130, 2809
BveI ACCTGC 1 cut(s) 2171
Cac8I GCNNGC 1 cut(s) 666
CaiI CAGNNNCTG 2 cut(s) 269, 2098
CfoI GCGC 2 cut(s) 294, 3522
Cfr13I GGNCC 9 cut(s) 147, 337, 338, 461, 1476, 1552, 1948, 2602, 3449
Cfr42I CCGCGG 1 cut(s) 830
CseI GACGC 2 cut(s) 395, 2690
CsiI ACCWGGT 1 cut(s) 3390
Csp6I GTAC 5 cut(s) 1516, 2324, 2959, 3388, 3574
CspCI CAANNNNNGTGG 2 cut(s) 3392, 3427
CviQI GTAC 5 cut(s) 1516, 2324, 2959, 3388, 3574
DraI TTTAAA 3 cut(s) 547, 622, 1035
DraIII CACNNNGTG 2 cut(s) 1838, 2036
DriI GACNNNNNGTC 1 cut(s) 2953
EaeI YGGCCR 1 cut(s) 1886
Eam1104I CTCTTC 5 cut(s) 168, 520, 2645, 3037, 3180
Eam1105I GACNNNNNGTC 1 cut(s) 2953
EarI CTCTTC 5 cut(s) 168, 520, 2645, 3037, 3180
Ecl136II GAGCTC 1 cut(s) 178
Eco130I CCWWGG 3 cut(s) 677, 752, 908
Eco147I AGGCCT 2 cut(s) 1924, 2563
Eco24I GRGCYC 2 cut(s) 180, 341
Eco47I GGWCC 4 cut(s) 147, 1552, 1948, 2602
Eco53kI GAGCTC 1 cut(s) 178
Eco57I CTGAAG 4 cut(s) 866, 1254, 1596, 3305
Eco88I CYCGRG 1 cut(s) 257
Eco91I GGTNACC 1 cut(s) 1348
EcoICRI GAGCTC 1 cut(s) 178
EcoNI CCTNNNNNAGG 2 cut(s) 1153, 2550
EcoO109I RGGNCCY 2 cut(s) 338, 2602
EcoO65I GGTNACC 1 cut(s) 1348
EcoRI GAATTC 2 cut(s) 346, 1396
EcoT14I CCWWGG 3 cut(s) 677, 752, 908
EcoT22I ATGCAT 1 cut(s) 238
EcoT38I GRGCYC 2 cut(s) 180, 341
ErhI CCWWGG 3 cut(s) 677, 752, 908
FaqI GGGAC 4 cut(s) 517, 1565, 2265, 2615
FauNDI CATATG 1 cut(s) 1684
FbaI TGATCA 4 cut(s) 712, 2404, 3142, 3346
FblI GTMKAC 7 cut(s) 90, 1594, 1679, 2230, 2357, 2966, 3227
FriOI GRGCYC 2 cut(s) 180, 341
FspBI CTAG 9 cut(s) 434, 780, 909, 1656, 1926, 1980, 2397, 2594, 2840
GlaI GCGC 2 cut(s) 293, 3521
GsaI CCCAGC 1 cut(s) 2659
GsuI CTGGAG 2 cut(s) 138, 3101
HaeII RGCGCY 2 cut(s) 295, 3523
HapII CCGG 2 cut(s) 1136, 1909
HgaI GACGC 2 cut(s) 395, 2690
HhaI GCGC 2 cut(s) 294, 3522
Hin1I GRCGYC 1 cut(s) 2701
Hin6I GCGC 2 cut(s) 292, 3520
HinP1I GCGC 2 cut(s) 292, 3520
HincII GTYRAC 3 cut(s) 299, 2223, 2893
HindII GTYRAC 3 cut(s) 299, 2223, 2893
HindIII AAGCTT 3 cut(s) 1196, 2577, 3472
HpaI GTTAAC 1 cut(s) 2893
HpaII CCGG 2 cut(s) 1136, 1909
HphI GGTGA 7 cut(s) 666, 1261, 1771, 1910, 2761, 3406, 3427
HpyCH4III ACNGT 6 cut(s) 31, 1556, 1693, 2378, 3168, 3278
HpyCH4IV ACGT 2 cut(s) 1638, 2644
HpySE526I ACGT 2 cut(s) 1638, 2644
Hsp92I GRCGYC 1 cut(s) 2701
HspAI GCGC 2 cut(s) 292, 3520
KpnI GGTACC 1 cut(s) 3391
Ksp22I TGATCA 4 cut(s) 712, 2404, 3142, 3346
KspAI GTTAAC 1 cut(s) 2893
KspI CCGCGG 1 cut(s) 830
LguI GCTCTTC 1 cut(s) 2645
LmnI GCTCC 5 cut(s) 1414, 2765, 2813, 3247, 3256
LweI GCATC 7 cut(s) 271, 1135, 2085, 2144, 2494, 2995, 3103
MabI ACCWGGT 1 cut(s) 3390
MaeI CTAG 9 cut(s) 434, 780, 909, 1656, 1926, 1980, 2397, 2594, 2840
MaeII ACGT 2 cut(s) 1638, 2644
MbiI CCGCTC 1 cut(s) 3259
MflI RGATCY 5 cut(s) 82, 490, 949, 1828, 3076
MhlI GDGCHC 4 cut(s) 180, 341, 3134, 3283
MlsI TGGCCA 1 cut(s) 1888
MluNI TGGCCA 1 cut(s) 1888
MlyI GAGTC 6 cut(s) 167, 269, 1952, 2979, 3341, 3536
MmeI TCCRAC 3 cut(s) 717, 1323, 1926
Mox20I TGGCCA 1 cut(s) 1888
Mph1103I ATGCAT 1 cut(s) 238
MroXI GAANNNNTTC 1 cut(s) 3293
MscI TGGCCA 1 cut(s) 1888
MslI CAYNNNNRTG 5 cut(s) 1221, 1484, 2111, 2526, 2571
Msp20I TGGCCA 1 cut(s) 1888
MspA1I CMGCKG 2 cut(s) 829, 3491
MspI CCGG 2 cut(s) 1136, 1909
Mva1269I GAATGC 2 cut(s) 1374, 3268
MvnI CGCG 1 cut(s) 829
NciI CCSGG 1 cut(s) 1136
NdeI CATATG 1 cut(s) 1684
NlaIV GGNNCC 9 cut(s) 149, 339, 462, 492, 1553, 1985, 2603, 2661, 3389
NmuCI GTSAC 9 cut(s) 265, 2588, 2614, 2707, 3056, 3196, 3333, 3394, 3423
NsiI ATGCAT 1 cut(s) 238
NspI RCATGY 6 cut(s) 236, 510, 2221, 2786, 2899, 3209
OliI CACNNNNGTG 1 cut(s) 2111
PceI AGGCCT 2 cut(s) 1924, 2563
PciI ACATGT 1 cut(s) 2782
PciSI GCTCTTC 1 cut(s) 2645
PctI GAATGC 2 cut(s) 1374, 3268
PdmI GAANNNNTTC 1 cut(s) 3293
PfeI GAWTC 5 cut(s) 913, 1207, 1602, 2458, 2488
PflMI CCANNNNNTGG 1 cut(s) 470
PfoI TCCNGGA 1 cut(s) 2473
PleI GAGTC 6 cut(s) 166, 268, 1951, 2979, 3340, 3536
PpsI GAGTC 6 cut(s) 166, 268, 1951, 2979, 3340, 3536
Ppu21I YACGTR 1 cut(s) 2645
PpuMI RGGWCCY 1 cut(s) 2602
PscI ACATGT 1 cut(s) 2782
PsiI TTATAA 1 cut(s) 2015
Psp124BI GAGCTC 1 cut(s) 180
Psp1406I AACGTT 1 cut(s) 1638
Psp5II RGGWCCY 1 cut(s) 2602
PspEI GGTNACC 1 cut(s) 1348
PspFI CCCAGC 1 cut(s) 2655
PspN4I GGNNCC 9 cut(s) 149, 339, 462, 492, 1553, 1985, 2603, 2661, 3389
PspOMI GGGCCC 1 cut(s) 337
PspPI GGNCC 9 cut(s) 147, 337, 338, 461, 1476, 1552, 1948, 2602, 3449
PspPPI RGGWCCY 1 cut(s) 2602
PstI CTGCAG 5 cut(s) 193, 2164, 2683, 3100, 3255
PstNI CAGNNNCTG 2 cut(s) 269, 2098
PsuI RGATCY 5 cut(s) 82, 490, 949, 1828, 3076
PvuII CAGCTG 1 cut(s) 3491
RsaI GTAC 5 cut(s) 1517, 2325, 2960, 3389, 3575
RsaNI GTAC 5 cut(s) 1516, 2324, 2959, 3388, 3574
RseI CAYNNNNRTG 5 cut(s) 1221, 1484, 2111, 2526, 2571
SacI GAGCTC 1 cut(s) 180
SacII CCGCGG 1 cut(s) 830
SapI GCTCTTC 1 cut(s) 2645
Sau96I GGNCC 9 cut(s) 147, 337, 338, 461, 1476, 1552, 1948, 2602, 3449
ScaI AGTACT 1 cut(s) 1517
SchI GAGTC 6 cut(s) 167, 269, 1952, 2979, 3341, 3536
SduI GDGCHC 4 cut(s) 180, 341, 3134, 3283
SexAI ACCWGGT 1 cut(s) 3390
SfaNI GCATC 7 cut(s) 271, 1135, 2085, 2144, 2494, 2995, 3103
SfcI CTRYAG 6 cut(s) 189, 2160, 2679, 3096, 3149, 3251
Sfr303I CCGCGG 1 cut(s) 830
SgrBI CCGCGG 1 cut(s) 830
SinI GGWCC 4 cut(s) 147, 1552, 1948, 2602
SmiMI CAYNNNNRTG 5 cut(s) 1221, 1484, 2111, 2526, 2571
SmlI CTYRAG 4 cut(s) 39, 308, 410, 2327
SmoI CTYRAG 4 cut(s) 39, 308, 410, 2327
SseBI AGGCCT 2 cut(s) 1924, 2563
SsiI CCGC 5 cut(s) 827, 829, 2238, 2800, 3259
SspI AATATT 3 cut(s) 982, 2052, 3538
SspMI CTAG 9 cut(s) 434, 780, 909, 1656, 1926, 1980, 2397, 2594, 2840
SstI GAGCTC 1 cut(s) 180
StuI AGGCCT 2 cut(s) 1924, 2563
StyI CCWWGG 3 cut(s) 677, 752, 908
TaaI ACNGT 6 cut(s) 31, 1556, 1693, 2378, 3168, 3278
TaiI ACGT 2 cut(s) 1641, 2647
TaqI TCGA 5 cut(s) 691, 893, 2727, 2820, 3309
TatI WGTACW 1 cut(s) 1515
TauI GCSGC 2 cut(s) 832, 2240
TfiI GAWTC 5 cut(s) 913, 1207, 1602, 2458, 2488
TseFI GTSAC 9 cut(s) 265, 2588, 2614, 2707, 3056, 3196, 3333, 3394, 3423
Tsp45I GTSAC 9 cut(s) 265, 2588, 2614, 2707, 3056, 3196, 3333, 3394, 3423
TspGWI ACGGA 1 cut(s) 1872
Van91I CCANNNNNTGG 1 cut(s) 470
VpaK11BI GGWCC 4 cut(s) 147, 1552, 1948, 2602
XagI CCTNNNNNAGG 2 cut(s) 1153, 2550
XapI RAATTY 7 cut(s) 346, 381, 512, 617, 1381, 1396, 2973
XceI RCATGY 6 cut(s) 236, 510, 2221, 2786, 2899, 3209
XmaJI CCTAGG 1 cut(s) 908
XmiI GTMKAC 7 cut(s) 90, 1594, 1679, 2230, 2357, 2966, 3227
XmnI GAANNNNTTC 1 cut(s) 3293
XspI CTAG 9 cut(s) 434, 780, 909, 1656, 1926, 1980, 2397, 2594, 2840
ZrmI AGTACT 1 cut(s) 1517
Zsp2I ATGCAT 1 cut(s) 238
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.