Rw0G000010
ERF Family

Probably involved in the RNA silencing pathway and required for the generation of small interfering RNAs (siRNAs)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00001
OG0008702
Physical Location & Seq
Reverse (-)
3808 .. 8232
4425 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G000010.1

Sequence Viewer

Length: 3228 bp
ATGGAATTAGAAGGAAGGGGAAATGACACAGTGGTAACTCAAGTCAGCTTTGGTGGATTTGGAAGTCATGTTAAAGCAAAAGATCTTGTAGACTACTTGGAAGATGAAATTGGGCTTGTATATAGGTGTCGGTTGAAAACTTCTTGGACCCCTCCAGAGTCTCTTCCGAGCTTTGAGCTCGTTGATACTGCAAATATTGAAACAACAGATGAATATAAAAAGGTTGAGCCACATGCATTTGTGCATTTTGCTTCGCCCGAGTCAGTGACTGCTGCTATGGATGCTGTTGGGCGCTGTCAACTCTTTCTCAAGAACCAAGCACTGAAAGTAAGTTTGGGCCCTGAGAATTCATACAACATGAACAAGAGGAGGAGGACTAAAATTCCATTCAAGTTAGCTGATGTGCGTCTTGAGATTGGAAGTTTAGTTCGCCTAGATGAGTTCTTTGTTGCTTGGAGGGGGCCATCTTATGGGGTTGATTTTGTTGTGGATCCTTTTGATGGGACATGCAAATTTTGTTTTAGAAGAGATACTGCCTTTTCATTTAAAGGGATGAATAAGTATGCTGTGATAAAATGCGATTTTAAGGTGGAGTTCCAGGTAAGAGAAATCAGTGAATTTAAACACTATACAGAGCCTTCATATCTTGTAATTCTGTTGAGGCTGGCTTCATCACCTTGGGTTTCATATCGAACTGCCGATGATGATATTGATCAATCAGTTCCATTTGATTTGTTGGATGACGATGATCCTTGGATTAGAACCACAGATTTTACTCCTAGTGGGGCAATCGGGCGTTGTAATTCTTACAGAGTTTCAATCCCACCGCGGCATGGTGCTAAGCTGAAGAAGGCCATGAGTTATCTTAAAGAACGCAGGGTGCAAGAGGTTTCGATCAAAAAGCCACCTAGGATTCAGAATGAACCTGATTTTGAAATGCCCATGTCGGATCCTTTCTTCTCTATTCATTACAAGGAAAATATTTCCTTTGAGATATTCACTTATGTTCATACAAACAACTTATGTTCATACAAACACCCGGTTTTTGATGCCTCTAAAAGGATGAAACTTGTCCAAGAATGGTTGCTGAGAAACCCCAAGCTTATCAAGAATCCCAAACATTTGGATGATATTGCTGAAGTTAGGAGGTTGGTGATTACCCCAACTAAAGCCTATTGTCTTCCACCAGAGGTTGAACTATCCAACAGGGTTCTTAGAAAATATAAAGAAGTTGCTGATAGGTTTTTAAGGGTTACCTTTATGGATGAAGGAATGCAGACAATAAATTCCAATGTTATGAATTCTTATGTTGCTCCTATTGTGAAGGAAATCACAGAAAACACCATCCCTCAGAAAACAAAAGGATTTCAATTATGTGGTCGTAAGTACTCGTTCTTGGCCTTCTCATCCAATCAACTGAGGGACCGTTCTGCCTGGTTTTTCGCTGAAGACAAGAAAATTAGTATACAAGGAATCAAAAATTGGATGGGGAAGTTCCACAACAGAAACGTTGCAAAATGTGCTGCTAGGATGGGTCAGTGTTTTTCGATCACCCCAGACCTTGCAATGGAAGTTGCAGAAAGACTGAAATTGGACAGAGATCCACCTTGTGCATATCAAATTAGATACGGAGGCTGTAAAGGGGTTATTGCTTGTTGGCCACCGACAGGTGATGGCTTCCGGCTTTCTTTGAGGCCTAGTATGAACAAGTTTGAGTCGGAGCATACTATATTGGAAATATGTTCATGGACTAGGTTCCAGCCAGGTTTCTTAAACAGGCAGATTATAACATTGCTTTCAGCACTTGGTGAGACAATGGTTTCCAAATTAAAGCAGATGCTGGTGGACACAGATCAAGGAAATGCTGCAGCTATAATGTTAAGTGCAGGTTTCAAGCCCCAGACAGAACCACATCTGAGAGGCATGTTGACCTGTATACAAGCCGCACAGCTCTGGGGACTTAGGGAAAAGGCCAGGATTTTTGTTCCTTCTGGGAGATGGCTTATGGGCTGTCTGGATGAGTTAGGGGTACTTGAGCAAGGCCAGTGCTTTGTCCAAGTGTCTACCCCATCGCTGGAAAACTGTTTTGCAAAGCATGGCTCTAGGTTTGATCAGATAGATAAAAATCTACAAGTAATAAAAGGGTTCGTAGTGGTAGCAAAGAATCCTTGCCTTCATCCAGGAGATATAAGGATTCTTGAAGCAGTTGATGCACCTGGGTTACACCACTTGCATGATTGCCTTGTTTTCCCTCAGAAAGGTCATAGGCCTCACACAGATGAAGCTTCTGGTAGTGACCTAGATGGGGACCTCTACTTTGTCACATGGGATGAAAATCTTATTCCCCCACGTAAGAAGAGCTGGGAACCTATGCAGTATGAAGCTGCAGAAGCCAAAGTTTCAGGGCGTCCTGTCACGAACCAGGATTTAATCGAGTTTTTTGTGAGAAACATGGTGAATGAGAACCTGGGAGCGATATGCAATGCACATGTGAAGTGCCTAAAACTAGCAGAATTAGCAGCCATAGCAGTTGATTTTCCCAAAACTGGGAAGATTCTATACCCAGATTTCATGGGAAAAGAGGACTACCAGTCGTACAAGTCTACTAAAATTTTAGGGAGACTCTATCGCAAGATTTGCGATGCTTATGAAGATGTGGCAACCTCTTCTGAGCAAAATCACAATCCCAGTGACATCAAATATGATATGGATCTGGAGGTTCCAAAAGCTGCAGATTTCATAGCTGATGCTTGGGAGAGAAAGTGCTCTTATGATGATCAGCTACAGGGTCTAATGGCACAGTATAAAGTTATGAGGGAAGAGGAGATTGTCACAGGGCATGTTTGGTCAATGCCCAAGTATACCAGCAGGAAGCAAGGGGAGCTGCAGGAGCGGTTGAGGCATTCTTACAGTGCTCTGAAGAAAGAGTTCAGGCAATTTTTCGAGAAGATGGATACGGGTTTTGAGTCACTGACTGATGATCAGAAAAATAGCTTGTATGAGCAGAAAGCATCAGCTTGGTACCAGGTGACTTACCACCCTGATTGGGTGAAAAGGTCACTCGATTTTCAAAGGCCAGATGGCCCAGGAGATGTGGTGATGTTAAGCTTTGCCTGGATTGCAGCTGACTACCTTGCTCGGATCAAGATCAAGCGCCGTGAAGTGGGTAATATTGACTCTAAGAAGCCAATCAATGCTTTGACGAAGTACCTTGCTGATCGGATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001172 GO:0002252 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0003968 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007275 GO:0008037 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009605 GO:0009607 GO:0009615 GO:0009616 GO:0009791 GO:0009856 GO:0009875 GO:0009888 GO:0009892 GO:0009908 GO:0009987 GO:0010033 GO:0010073 GO:0010074 GO:0010267 GO:0010467 GO:0010468 GO:0010492 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019222 GO:0019438 GO:0019827 GO:0022414 GO:0030422 GO:0031047 GO:0031050 GO:0032501 GO:0032502 GO:0032774 GO:0034062 GO:0034641 GO:0034654 GO:0035194 GO:0035821 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043331 GO:0044003 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044403 GO:0044419 GO:0044424 GO:0044464 GO:0044706 GO:0045087 GO:0046483 GO:0048366 GO:0048367 GO:0048437 GO:0048438 GO:0048440 GO:0048467 GO:0048507 GO:0048519 GO:0048544 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050896 GO:0051607 GO:0051701 GO:0051704 GO:0051707 GO:0051716 GO:0051817 GO:0052018 GO:0052249 GO:0060255 GO:0061458 GO:0065007 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0090567 GO:0097659 GO:0097747 GO:0098542 GO:0098586 GO:0098727 GO:0099402 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1901698 GO:1901699
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RdRP

Protein Analysis

1075

Amino Acids

123.23

Weight (kDa)

8.28

Isoelectric Point (pI)

32.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RBD_RDR6 PF24572 11 - 111 2.4e-33 RNA-dependent RNA polymerase 6, RNA-binding domain
RDR6_2nd PF24577 131 - 294 1.8e-84 RNA-dependent RNA polymerase 6 second domain
PH_RDR2 PF24823 194 - 290 3.4e-06 RDR2 PH-like domain
RdRP PF05183 386 - 519 2e-37 RNA dependent RNA polymerase
RdRP PF05183 517 - 879 2.7e-105 RNA dependent RNA polymerase
RdRP_head PF26253 901 - 1063 2.2e-45 RdRP, head domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1787
Acc36I ACCTGC 1 cut(s) 1877
Acc65I GGTACC 1 cut(s) 3021
AccB1I GGYRCC 1 cut(s) 3021
AccB7I CCANNNNNTGG 1 cut(s) 470
AccBSI CCGCTC 1 cut(s) 2893
AccI GTMKAC 6 cut(s) 90, 1465, 1936, 2063, 2603, 2861
AccII CGCG 1 cut(s) 829
AciI CCGC 4 cut(s) 827, 829, 1944, 2893
AclI AACGTT 1 cut(s) 1509
AclWI GGATC 8 cut(s) 485, 498, 743, 944, 957, 1595, 2718, 3149
AcoI YGGCCR 1 cut(s) 1658
AcsI RAATTY 7 cut(s) 346, 381, 512, 617, 1285, 1300, 2610
AcuI CTGAAG 4 cut(s) 866, 1158, 1467, 2939
AcyI GRCGYC 1 cut(s) 2407
AdeI CACNNNGTG 2 cut(s) 1610, 1808
AfaI GTAC 5 cut(s) 1388, 2031, 2597, 3023, 3209
AflIII ACRYGT 1 cut(s) 2488
AhdI GACNNNNNGTC 1 cut(s) 2590
AjuI GAANNNNNNNTTGG 6 cut(s) 93, 125, 317, 349, 1465, 1497
AloI GAACNNNNNNTCC 2 cut(s) 411, 443
Alw21I GWGCWC 3 cut(s) 180, 2768, 2917
Alw26I GTCTC 3 cut(s) 165, 1805, 2614
AlwI GGATC 8 cut(s) 485, 498, 743, 944, 957, 1595, 2718, 3149
AlwNI CAGNNNCTG 2 cut(s) 269, 1840
Ama87I CYCGRG 1 cut(s) 257
ApaI GGGCCC 1 cut(s) 341
ApeKI GCWGC 9 cut(s) 272, 1523, 1865, 1868, 2384, 2519, 2729, 2884, 3122
ApoI RAATTY 7 cut(s) 346, 381, 512, 617, 1285, 1300, 2610
ArsI GACNNNNNNTTYG 2 cut(s) 2300, 2332
Asp700I GAANNNNTTC 1 cut(s) 2927
Asp718I GGTACC 1 cut(s) 3021
AspA2I CCTAGG 1 cut(s) 908
AspLEI GCGC 2 cut(s) 294, 3156
AspS9I GGNCC 7 cut(s) 147, 337, 338, 461, 1423, 2308, 3083
AsuC2I CCSGG 1 cut(s) 1040
AsuHPI GGTGA 9 cut(s) 666, 1165, 1543, 1682, 1820, 2467, 3040, 3061, 3109
AvaI CYCGRG 1 cut(s) 257
AvaII GGWCC 3 cut(s) 147, 1423, 2308
AvrII CCTAGG 1 cut(s) 908
BaeGI GKGCMC 1 cut(s) 341
BalI TGGCCA 1 cut(s) 1660
BamHI GGATCC 2 cut(s) 490, 949
BanI GGYRCC 1 cut(s) 3021
BanII GRGCYC 2 cut(s) 180, 341
BarI GAAGNNNNNNTAC 2 cut(s) 2543, 2575
BbsI GAAGAC 2 cut(s) 1172, 1455
Bbv12I GWGCWC 3 cut(s) 180, 2768, 2917
BbvI GCAGC 9 cut(s) 259, 1510, 1852, 1880, 2371, 2531, 2716, 2871, 3134
BceAI ACGGC 1 cut(s) 3141
BciVI GTATCC 1 cut(s) 2947
BclI TGATCA 4 cut(s) 712, 2110, 2776, 2980
BcnI CCSGG 1 cut(s) 1040
BcoDI GTCTC 3 cut(s) 165, 1805, 2614
BfaI CTAG 9 cut(s) 434, 780, 909, 1527, 1698, 1752, 2103, 2300, 2507
BfmI CTRYAG 5 cut(s) 1866, 2385, 2730, 2783, 2885
BfoI RGCGCY 2 cut(s) 295, 3157
BfuAI ACCTGC 1 cut(s) 1877
BfuI GTATCC 1 cut(s) 2947
BglII AGATCT 1 cut(s) 82
BlnI CCTAGG 1 cut(s) 908
BlpI GCTNAGC 1 cut(s) 840
BmcAI AGTACT 1 cut(s) 1388
Bme18I GGWCC 3 cut(s) 147, 1423, 2308
BmeRI GACNNNNNGTC 1 cut(s) 2590
BmeT110I CYCGRG 1 cut(s) 257
BmgT120I GGNCC 7 cut(s) 147, 337, 338, 461, 1423, 2308, 3083
BmrI ACTGGG 2 cut(s) 2556, 2682
BmsI GCATC 7 cut(s) 271, 1039, 1827, 2200, 2632, 2737, 3020
BmuI ACTGGG 2 cut(s) 2556, 2682
BpiI GAAGAC 2 cut(s) 1172, 1455
BpmI CTGGAG 2 cut(s) 138, 2735
Bpu1102I GCTNAGC 1 cut(s) 840
BpuEI CTTGAG 4 cut(s) 24, 293, 431, 2054
BpuMI CCSGG 1 cut(s) 1040
BsaAI YACGTR 1 cut(s) 2351
BsaBI GATNNNNATC 5 cut(s) 711, 2124, 2334, 2709, 3146
BsaHI GRCGYC 1 cut(s) 2407
BsaJI CCNNGG 7 cut(s) 677, 752, 827, 908, 2216, 2467, 3085
BsaXI ACNNNNNCTCC 4 cut(s) 2747, 2777, 3081, 3111
Bse1I ACTGG 4 cut(s) 2044, 2551, 2590, 2688
Bse3DI GCAATG 3 cut(s) 1572, 1790, 2488
Bse8I GATNNNNATC 5 cut(s) 711, 2124, 2334, 2709, 3146
BseDI CCNNGG 7 cut(s) 677, 752, 827, 908, 2216, 2467, 3085
BseJI GATNNNNATC 5 cut(s) 711, 2124, 2334, 2709, 3146
BseMI GCAATG 3 cut(s) 1572, 1790, 2488
BseMII CTCAG 7 cut(s) 333, 1079, 1364, 1409, 1907, 2267, 2661
BseNI ACTGG 4 cut(s) 2044, 2551, 2590, 2688
BseRI GAGGAG 3 cut(s) 382, 385, 2837
BseSI GKGCMC 1 cut(s) 341
BseXI GCAGC 9 cut(s) 259, 1510, 1852, 1880, 2371, 2531, 2716, 2871, 3134
BseYI CCCAGC 1 cut(s) 2361
BsgI GTGCAG 1 cut(s) 1905
Bsh1236I CGCG 1 cut(s) 829
BshNI GGYRCC 1 cut(s) 3021
BsiHKAI GWGCWC 3 cut(s) 180, 2768, 2917
BsiHKCI CYCGRG 1 cut(s) 257
BsiSI CCGG 2 cut(s) 1040, 1681
BslFI GGGAC 4 cut(s) 517, 1436, 1971, 2321
BsmAI GTCTC 3 cut(s) 165, 1805, 2614
BsmFI GGGAC 4 cut(s) 517, 1436, 1971, 2321
BsmI GAATGC 2 cut(s) 1278, 2902
BsoBI CYCGRG 1 cut(s) 257
Bsp120I GGGCCC 1 cut(s) 337
Bsp1286I GDGCHC 4 cut(s) 180, 341, 2768, 2917
Bsp1720I GCTNAGC 1 cut(s) 840
BspACI CCGC 4 cut(s) 827, 829, 1944, 2893
BspCNI CTCAG 7 cut(s) 334, 1080, 1363, 1410, 1908, 2266, 2662
BspFNI CGCG 1 cut(s) 829
BspMAI CTGCAG 4 cut(s) 1870, 2389, 2734, 2889
BspMI ACCTGC 1 cut(s) 1877
BspPI GGATC 8 cut(s) 485, 498, 743, 944, 957, 1595, 2718, 3149
BspQI GCTCTTC 1 cut(s) 2351
BspT107I GGYRCC 1 cut(s) 3021
BsrBI CCGCTC 1 cut(s) 2893
BsrDI GCAATG 3 cut(s) 1572, 1790, 2488
BsrI ACTGG 4 cut(s) 2044, 2551, 2590, 2688
BssECI CCNNGG 7 cut(s) 677, 752, 827, 908, 2216, 2467, 3085
BssNAI GTATAC 3 cut(s) 1466, 1937, 2862
BssNI GRCGYC 1 cut(s) 2407
BssT1I CCWWGG 3 cut(s) 677, 752, 908
Bst1107I GTATAC 3 cut(s) 1466, 1937, 2862
Bst4CI ACNGT 5 cut(s) 31, 1427, 2084, 2802, 2912
Bst6I CTCTTC 5 cut(s) 168, 520, 2351, 2671, 2814
BstACI GRCGYC 1 cut(s) 2407
BstAPI GCANNNNNTGC 3 cut(s) 1520, 2210, 2637
BstBAI YACGTR 1 cut(s) 2351
BstC8I GCNNGC 1 cut(s) 666
BstDSI CCRYGG 1 cut(s) 827
BstEII GGTNACC 1 cut(s) 1252
BstENI CCTNNNNNAGG 2 cut(s) 1057, 2256
BstFNI CGCG 1 cut(s) 829
BstH2I RGCGCY 2 cut(s) 295, 3157
BstHHI GCGC 2 cut(s) 294, 3156
BstMAI GTCTC 3 cut(s) 165, 1805, 2614
BstNSI RCATGY 5 cut(s) 236, 510, 1927, 2492, 2843
BstPI GGTNACC 1 cut(s) 1252
BstSFI CTRYAG 5 cut(s) 1866, 2385, 2730, 2783, 2885
BstSLI GKGCMC 1 cut(s) 341
BstUI CGCG 1 cut(s) 829
BstV1I GCAGC 9 cut(s) 259, 1510, 1852, 1880, 2371, 2531, 2716, 2871, 3134
BstV2I GAAGAC 2 cut(s) 1172, 1455
BstX2I RGATCY 5 cut(s) 82, 490, 949, 1600, 2710
BstXI CCANNNNNNTGG 1 cut(s) 1123
BstYI RGATCY 5 cut(s) 82, 490, 949, 1600, 2710
BstZ17I GTATAC 3 cut(s) 1466, 1937, 2862
BsuI GTATCC 1 cut(s) 2947
BtgI CCRYGG 1 cut(s) 827
BtgZI GCGATG 2 cut(s) 2055, 2655
BtsIMutI CAGTG 9 cut(s) 36, 270, 320, 619, 1544, 2051, 2695, 2917, 2969
BveI ACCTGC 1 cut(s) 1877
Cac8I GCNNGC 1 cut(s) 666
CaiI CAGNNNCTG 2 cut(s) 269, 1840
CfoI GCGC 2 cut(s) 294, 3156
Cfr13I GGNCC 7 cut(s) 147, 337, 338, 461, 1423, 2308, 3083
Cfr42I CCGCGG 1 cut(s) 830
CseI GACGC 2 cut(s) 395, 2396
CsiI ACCWGGT 1 cut(s) 3024
Csp6I GTAC 5 cut(s) 1387, 2030, 2596, 3022, 3208
CspCI CAANNNNNGTGG 2 cut(s) 3026, 3061
CviQI GTAC 5 cut(s) 1387, 2030, 2596, 3022, 3208
DraI TTTAAA 2 cut(s) 547, 622
DraIII CACNNNGTG 2 cut(s) 1610, 1808
DriI GACNNNNNGTC 1 cut(s) 2590
EaeI YGGCCR 1 cut(s) 1658
Eam1104I CTCTTC 5 cut(s) 168, 520, 2351, 2671, 2814
Eam1105I GACNNNNNGTC 1 cut(s) 2590
EarI CTCTTC 5 cut(s) 168, 520, 2351, 2671, 2814
Ecl136II GAGCTC 1 cut(s) 178
Eco130I CCWWGG 3 cut(s) 677, 752, 908
Eco147I AGGCCT 2 cut(s) 1696, 2269
Eco24I GRGCYC 2 cut(s) 180, 341
Eco47I GGWCC 3 cut(s) 147, 1423, 2308
Eco53kI GAGCTC 1 cut(s) 178
Eco57I CTGAAG 4 cut(s) 866, 1158, 1467, 2939
Eco88I CYCGRG 1 cut(s) 257
Eco91I GGTNACC 1 cut(s) 1252
EcoICRI GAGCTC 1 cut(s) 178
EcoNI CCTNNNNNAGG 2 cut(s) 1057, 2256
EcoO109I RGGNCCY 2 cut(s) 338, 2308
EcoO65I GGTNACC 1 cut(s) 1252
EcoRI GAATTC 2 cut(s) 346, 1300
EcoT14I CCWWGG 3 cut(s) 677, 752, 908
EcoT22I ATGCAT 1 cut(s) 238
EcoT38I GRGCYC 2 cut(s) 180, 341
ErhI CCWWGG 3 cut(s) 677, 752, 908
FaqI GGGAC 4 cut(s) 517, 1436, 1971, 2321
FbaI TGATCA 4 cut(s) 712, 2110, 2776, 2980
FblI GTMKAC 6 cut(s) 90, 1465, 1936, 2063, 2603, 2861
FriOI GRGCYC 2 cut(s) 180, 341
FspBI CTAG 9 cut(s) 434, 780, 909, 1527, 1698, 1752, 2103, 2300, 2507
GlaI GCGC 2 cut(s) 293, 3155
GsaI CCCAGC 1 cut(s) 2365
GsuI CTGGAG 2 cut(s) 138, 2735
HaeII RGCGCY 2 cut(s) 295, 3157
HapII CCGG 2 cut(s) 1040, 1681
HgaI GACGC 2 cut(s) 395, 2396
HhaI GCGC 2 cut(s) 294, 3156
Hin1I GRCGYC 1 cut(s) 2407
Hin6I GCGC 2 cut(s) 292, 3154
HinP1I GCGC 2 cut(s) 292, 3154
HincII GTYRAC 2 cut(s) 299, 1929
HindII GTYRAC 2 cut(s) 299, 1929
HindIII AAGCTT 3 cut(s) 1100, 2283, 3106
HpaII CCGG 2 cut(s) 1040, 1681
HphI GGTGA 9 cut(s) 666, 1165, 1543, 1682, 1820, 2467, 3040, 3061, 3109
Hpy166II GTNNAC 9 cut(s) 91, 299, 1466, 1846, 1929, 1937, 2064, 2604, 2862
Hpy8I GTNNAC 9 cut(s) 91, 299, 1466, 1846, 1929, 1937, 2064, 2604, 2862
HpyAV CCTTC 9 cut(s) 5, 9, 648, 844, 1262, 1318, 1411, 1998, 2183
HpyCH4III ACNGT 5 cut(s) 31, 1427, 2084, 2802, 2912
HpyCH4IV ACGT 2 cut(s) 1509, 2350
HpySE526I ACGT 2 cut(s) 1509, 2350
Hsp92I GRCGYC 1 cut(s) 2407
HspAI GCGC 2 cut(s) 292, 3154
KpnI GGTACC 1 cut(s) 3025
Ksp22I TGATCA 4 cut(s) 712, 2110, 2776, 2980
KspI CCGCGG 1 cut(s) 830
LguI GCTCTTC 1 cut(s) 2351
LmnI GCTCC 5 cut(s) 1318, 1720, 2471, 2881, 2890
Lsp1109I GCAGC 9 cut(s) 259, 1510, 1852, 1880, 2371, 2531, 2716, 2871, 3134
LweI GCATC 7 cut(s) 271, 1039, 1827, 2200, 2632, 2737, 3020
MabI ACCWGGT 1 cut(s) 3024
MaeI CTAG 9 cut(s) 434, 780, 909, 1527, 1698, 1752, 2103, 2300, 2507
MaeII ACGT 2 cut(s) 1509, 2350
MbiI CCGCTC 1 cut(s) 2893
MflI RGATCY 5 cut(s) 82, 490, 949, 1600, 2710
MhlI GDGCHC 4 cut(s) 180, 341, 2768, 2917
MlsI TGGCCA 1 cut(s) 1660
MluNI TGGCCA 1 cut(s) 1660
MlyI GAGTC 6 cut(s) 167, 269, 1724, 2616, 2975, 3170
MmeI TCCRAC 4 cut(s) 717, 927, 1227, 1698
Mox20I TGGCCA 1 cut(s) 1660
Mph1103I ATGCAT 1 cut(s) 238
MroXI GAANNNNTTC 1 cut(s) 2927
MscI TGGCCA 1 cut(s) 1660
MslI CAYNNNNRTG 3 cut(s) 1125, 2232, 2277
Msp20I TGGCCA 1 cut(s) 1660
MspA1I CMGCKG 2 cut(s) 829, 3125
MspI CCGG 2 cut(s) 1040, 1681
Mva1269I GAATGC 2 cut(s) 1278, 2902
MvnI CGCG 1 cut(s) 829
NciI CCSGG 1 cut(s) 1040
NmuCI GTSAC 9 cut(s) 265, 2294, 2320, 2413, 2690, 2830, 2967, 3028, 3057
NsiI ATGCAT 1 cut(s) 238
NspI RCATGY 5 cut(s) 236, 510, 1927, 2492, 2843
PceI AGGCCT 2 cut(s) 1696, 2269
PciI ACATGT 1 cut(s) 2488
PciSI GCTCTTC 1 cut(s) 2351
PctI GAATGC 2 cut(s) 1278, 2902
PdmI GAANNNNTTC 1 cut(s) 2927
PfeI GAWTC 6 cut(s) 913, 1111, 1473, 2164, 2194, 2554
PflMI CCANNNNNTGG 1 cut(s) 470
PfoI TCCNGGA 1 cut(s) 2179
PleI GAGTC 6 cut(s) 166, 268, 1723, 2616, 2974, 3170
PpsI GAGTC 6 cut(s) 166, 268, 1723, 2616, 2974, 3170
Ppu21I YACGTR 1 cut(s) 2351
PpuMI RGGWCCY 1 cut(s) 2308
PscI ACATGT 1 cut(s) 2488
PsiI TTATAA 1 cut(s) 1787
Psp124BI GAGCTC 1 cut(s) 180
Psp1406I AACGTT 1 cut(s) 1509
Psp5II RGGWCCY 1 cut(s) 2308
PspEI GGTNACC 1 cut(s) 1252
PspFI CCCAGC 1 cut(s) 2361
PspOMI GGGCCC 1 cut(s) 337
PspPI GGNCC 7 cut(s) 147, 337, 338, 461, 1423, 2308, 3083
PspPPI RGGWCCY 1 cut(s) 2308
PstI CTGCAG 4 cut(s) 1870, 2389, 2734, 2889
PstNI CAGNNNCTG 2 cut(s) 269, 1840
PsuI RGATCY 5 cut(s) 82, 490, 949, 1600, 2710
PvuII CAGCTG 1 cut(s) 3125
RsaI GTAC 5 cut(s) 1388, 2031, 2597, 3023, 3209
RsaNI GTAC 5 cut(s) 1387, 2030, 2596, 3022, 3208
RseI CAYNNNNRTG 3 cut(s) 1125, 2232, 2277
SacI GAGCTC 1 cut(s) 180
SacII CCGCGG 1 cut(s) 830
SapI GCTCTTC 1 cut(s) 2351
Sau96I GGNCC 7 cut(s) 147, 337, 338, 461, 1423, 2308, 3083
ScaI AGTACT 1 cut(s) 1388
SchI GAGTC 6 cut(s) 167, 269, 1724, 2616, 2975, 3170
SduI GDGCHC 4 cut(s) 180, 341, 2768, 2917
SexAI ACCWGGT 1 cut(s) 3024
SfaNI GCATC 7 cut(s) 271, 1039, 1827, 2200, 2632, 2737, 3020
SfcI CTRYAG 5 cut(s) 1866, 2385, 2730, 2783, 2885
Sfr303I CCGCGG 1 cut(s) 830
SgrBI CCGCGG 1 cut(s) 830
SinI GGWCC 3 cut(s) 147, 1423, 2308
SmiMI CAYNNNNRTG 3 cut(s) 1125, 2232, 2277
SmlI CTYRAG 4 cut(s) 39, 308, 410, 2033
SmoI CTYRAG 4 cut(s) 39, 308, 410, 2033
SseBI AGGCCT 2 cut(s) 1696, 2269
SsiI CCGC 4 cut(s) 827, 829, 1944, 2893
SspI AATATT 3 cut(s) 196, 982, 3172
SspMI CTAG 9 cut(s) 434, 780, 909, 1527, 1698, 1752, 2103, 2300, 2507
SstI GAGCTC 1 cut(s) 180
StuI AGGCCT 2 cut(s) 1696, 2269
StyI CCWWGG 3 cut(s) 677, 752, 908
TaaI ACNGT 5 cut(s) 31, 1427, 2084, 2802, 2912
TaiI ACGT 2 cut(s) 1512, 2353
TaqI TCGA 6 cut(s) 691, 893, 1547, 2433, 2943, 3063
TatI WGTACW 1 cut(s) 1386
TauI GCSGC 2 cut(s) 832, 1946
TfiI GAWTC 6 cut(s) 913, 1111, 1473, 2164, 2194, 2554
TscAI CASTG 9 cut(s) 36, 270, 327, 619, 1544, 2051, 2695, 2917, 2976
TseFI GTSAC 9 cut(s) 265, 2294, 2320, 2413, 2690, 2830, 2967, 3028, 3057
TseI GCWGC 9 cut(s) 272, 1523, 1865, 1868, 2384, 2519, 2729, 2884, 3122
Tsp45I GTSAC 9 cut(s) 265, 2294, 2320, 2413, 2690, 2830, 2967, 3028, 3057
TspGWI ACGGA 1 cut(s) 1644
TspRI CASTG 9 cut(s) 36, 270, 327, 619, 1544, 2051, 2695, 2917, 2976
Van91I CCANNNNNTGG 1 cut(s) 470
VpaK11BI GGWCC 3 cut(s) 147, 1423, 2308
XagI CCTNNNNNAGG 2 cut(s) 1057, 2256
XapI RAATTY 7 cut(s) 346, 381, 512, 617, 1285, 1300, 2610
XceI RCATGY 5 cut(s) 236, 510, 1927, 2492, 2843
XmaJI CCTAGG 1 cut(s) 908
XmiI GTMKAC 6 cut(s) 90, 1465, 1936, 2063, 2603, 2861
XmnI GAANNNNTTC 1 cut(s) 2927
XspI CTAG 9 cut(s) 434, 780, 909, 1527, 1698, 1752, 2103, 2300, 2507
ZrmI AGTACT 1 cut(s) 1388
Zsp2I ATGCAT 1 cut(s) 238
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.