Rh4CG182500
ERF Family

Probably involved in the RNA silencing pathway and required for the generation of small interfering RNAs (siRNAs)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0008702
Physical Location & Seq
Forward (+)
39413681 .. 39425255
11575 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG182500.1

Sequence Viewer

Length: 3657 bp
ATGATTGAATCTCGAACAATGCAAGAGAAGCCACATATCTCGAAATATCAAGTCTCGGGGAAGATGGAATTAAAAGGAAGGGAAAATGACACAGTGGTAACTCAAGTCAGCTTTGGTGGATTTGGAAGTCATGTTAAAGCAAAAGATCTTGTAGACTACTTGGAAGATGAAATTGGGCTTGTATATAGGTGTCGGTTGAAAATTTCTTGGACCCCTCCAGAGTCTCTTCCGAGCTTTGAGCTCGTTGATACTGCAGATATTGAAACAACAAATGAATATAAAAAGGTTGAGCCACATGCATTTGTGCATTTTGCTTCGCCCGAGTCAGTGACTGCTGCTATGGATGCTGTTGGGCGCTGTCAACTCTTTCTCAAGAACCAAGCACTGAAAGTAAGTTTGGGCCCTGAGAATTCATACAACATGAACAAGAGGAGGAGGACTAAAATTCCATTCAAGTTAGCTGATGTGCGTCTTGAGATTGGAAGTTTAGTTCGCCTAGATGAGTTCTTTGTTGCTTGGAGGGGGCCATCTTATGGGGTTGATTTTGTTGTGGATCCTTTTGATGGGACATGCAAATTTTGTTTTAGAAGAGATACTGCCTTTTCATTTAAAGGGATGAATAAGTATGCTGTGATAAAATGCGATTTTAAGGTGGAGTTCCAGGTAAGAGAAATCAGTGAATTTAAACACTATACAGAGCCTTCATATCTTGTAATTCTGTTGAGGCTGGCTTCATCACCTTGGGTTTCATATCGAACTGCCGATGATGATATTGATCAATCAGTTCCATTTGATTTGTTGGATGACGATGATCCTTGGATTAGAACCACAGATTTTACTCCTAGTGGGGCAATCGGGCGTTGTAATTCTTACAGAGTTTCAATCCCACCGCGGCATGGTGCTAAGCTGAAGAAGGCCATGAGTTATCTTAAAGAACGCAGGGTGCAAGAGGTTTCGATCAAAAAGCCACCTAGGATTCAGAATGAACCTGATTTTGAAATGCCTATGTCGGATCCTTTCTTCTCTATTCATTACAAGGAAAATATTTCCTTTGAGATATTGTTCTTGGTCAATGCTGTAATGCACAGGGGCATTTTAAATCAGCACCAGTTGTCAGACGGCTTTTTTGATTTACTGAGAAACCAACCAAAGGAGATCAATGTGGCTGCATTGAAGCACTTATGTTCATACAAACACCCGGTTTTTGATGCCTCTAAAAGGCTGAAACTTGTCCAAGAATGGTTGCTGAGAAACCCCAAGCTTATCAAGAATCCCAAACATTTGGATGATATTGCTGAAGTTAGGAGGTTGGTGATTACCCCAACTAAAGCCTATTGTCTTCCACCAGAGGCTGAACTATCCAACAGGGTTCTTAGAAAATATAAAGAAGTTGCTGATAGGTTTTTAAGGGTTACCTTTATGGATGAAGGAATGCAGACAATAAATTCCAATGTTATGAATTCTTATGTTGCTCCTATTGTGAAGGAAATCACAGAAAACACCATCCCTCAGAAAACAAAAGTGTATGCAAGGGTTCGGGCCATTCTGATGAAGGGATTTCAATTATGTGGTCGTAAGTACTCGTTCTTGGCCTTCTCATCCAATCAACTGAGGGACCGTTCTGCCTGGTTTTTCGCTGAAGACAAGAAGATTAGTATACAAGGAATCAAAAATTGGATGGGGAAGTTCCACAACAGAAACGTTGCAAAATGTGCTGCTAGGATGGGTCAGTGTTTTTCGTCTACATATGCAACGGTAGAAGTTCCATCAGCCCAGGTTAATCCTAAACTGCCAGATATTGAGAGAAATGACTATATCTTTTCTGATGGTATTGGTAGGATCACCCCAGACCTTGCAATGGAAGTTGCAGAAAGACTGAAATTGGACAGAGATCCACCTTGTGCATATCAAATTAGATACGGAGGCTGTAAAGGGGTTATTGCTTGTTGGCCACCGACAGGTGATGGCTTCCGGCTTTCTTTGAGGCCTAGTATGAACAAGTTTGAGTCGGAGCATACGATATTGGAAATATGTTCATGGACTAGGTTCCAGCCAGGTTTCTTAAACAGGCAGATTATAACATTGCTTTCAGCACTTGGTGTTCCAGATGAAATATTCTGGACAATGCAGGAGACAATGGTTTCCAAATTAAAGCAGATGCTGGTGGACACAGATGTGGCGTTTGATGTTCTCACTGCATCCTGTGCTGAGCAAGGAAATGCTGCAGCTATAATGTTAAGTGCAGGTTTCAAGCCCCAGACAGAACCACATCTGAGAGGCATGTTGACCTGTATACAAGCCGCACAGCTCTGGGGACTTAGGGAAAAGGCCAGGATTTTTGTTCCTTCTGGGAGATGGCTTATGGGCTGTCTGGATGAGTTAGGGGTACTTGAGCAAGGCCAGTGCTTTGTCCAAGTGTCTACCCCATCGCTGGAAAACTGTTTTGCAAAGCATGGCTCTAGGTTTGATCAGATAGATAAAAATCTACAAGTAATAAAAGGGTTCGTAGTGGTAGCAAAGAATCCTTGCCTTCATCCAGGAGATATAAGGATTCTTGAAGCAGTTGATGCACCTGGGTTACACCACTTGCATGATTGCCTTGTTTTCCCTCAGAAAGGTCATAGGCCTCACACAGATGAAGCTTCTGGTAGTGACCTAGATGGGGACCTCTACTTTGTCACATGGGATGAAAATCTTATTCCCCCACGTAAGAAGAGCTGGGAACCTATGCAGTATGAAGCTGCAGAAGCCAAAATTTCAGGGCGTCCTGTCACAAACCAGGATTTAATCGAGTTTTTTGTGAGAAACATGGTGAATGAGAACCTGGGAGCGATATGCAATGCACATGTGGTTCATTCTGACCGCAGTGAATATGGAGCTTTCGATGAGAAGTGCCTAAAACTAGCAGAATTAGCAGCCATAGCAGTTGATTTTCCCAAAACTGGGAAGATTGTTAACATGCCACCACCCCTAAAGCCAAAGCTATACCCAGATTTCATGGGAAAAGAGGACTACCAGTCGTACAAGTCTACTAAAATTTTAGGGAGACTCTATCGCAAGATTTGCGATGCTTATGATGAAGATGTGGCAACCTCTTCTGAGCAAAATCACAATCCCAGTGACATCAAATATGATATGGATCTGGAGGTTCCAAAAGCTGCAGATTTCATAGCTGATGCTTGGGAGAGAAAGTGCTCTTATGATGATCAGCTACAGGGTCTAATGGCACAGTATAAAGTTATGAGGGAAGAGGAGATTGTCACAGGGCATGTTTGGTCAATGCCCAAGTATACCAGCAGGAAGCAAGGGGAGCTGCAGGAGCGGTTGAGGCATTCTTACAGTGCTCTGAAGAAAGAGTTCAGGCAATTTTTCGAGAAGATGGATACGGGTTTTGAGTCACTGACTGATGATCAGAAAAATAGCTTGTATGAGCAGAAAGCATCAGCTTGGTACCAGGTGACTTACCACCCTGATTGGGTGAAAAGGTCACTCGATTTTCAAAGGCCAGATGGCCCAGGAGATGTGGTGATGTTAAGCTTTGCCTGGATTGCAGCTGACTACCTTGCTCGGATCAAGATCAAGCGCCGTGAAGTGGGTAATATTGACTCTAAGAAGCCAATCAATGCTTTGACGAAGTACCTTGCTGATCGGATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001172 GO:0002252 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0003968 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007275 GO:0008037 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009605 GO:0009607 GO:0009615 GO:0009616 GO:0009791 GO:0009856 GO:0009875 GO:0009888 GO:0009892 GO:0009908 GO:0009987 GO:0010033 GO:0010073 GO:0010074 GO:0010267 GO:0010467 GO:0010468 GO:0010492 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019222 GO:0019438 GO:0019827 GO:0022414 GO:0030422 GO:0031047 GO:0031050 GO:0032501 GO:0032502 GO:0032774 GO:0034062 GO:0034641 GO:0034654 GO:0035194 GO:0035821 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043331 GO:0044003 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044403 GO:0044419 GO:0044424 GO:0044464 GO:0044706 GO:0045087 GO:0046483 GO:0048366 GO:0048367 GO:0048437 GO:0048438 GO:0048440 GO:0048467 GO:0048507 GO:0048519 GO:0048544 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050896 GO:0051607 GO:0051701 GO:0051704 GO:0051707 GO:0051716 GO:0051817 GO:0052018 GO:0052249 GO:0060255 GO:0061458 GO:0065007 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0090567 GO:0097659 GO:0097747 GO:0098542 GO:0098586 GO:0098727 GO:0099402 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1901698 GO:1901699
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RdRP

Protein Analysis

1218

Amino Acids

139.44

Weight (kDa)

8.17

Isoelectric Point (pI)

34.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RBD_RDR6 PF24572 31 - 132 3.9e-32 RNA-dependent RNA polymerase 6, RNA-binding domain
RDR6_2nd PF24577 152 - 315 2.2e-84 RNA-dependent RNA polymerase 6 second domain
PH_RDR2 PF24823 215 - 311 4e-06 RDR2 PH-like domain
RdRP_helical PF26252 339 - 421 2.9e-23 RNA dependent RNA polymerase, helical domain
RdRP PF05183 439 - 1021 4.8e-195 RNA dependent RNA polymerase
RdRP_head PF26253 1044 - 1206 2.7e-45 RdRP, head domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2078
Acc36I ACCTGC 1 cut(s) 2234
Acc65I GGTACC 1 cut(s) 3450
AccB1I GGYRCC 1 cut(s) 3450
AccB7I CCANNNNNTGG 1 cut(s) 533
AccBSI CCGCTC 1 cut(s) 3322
AccI GTMKAC 7 cut(s) 153, 1657, 1742, 2293, 2420, 3029, 3290
AccII CGCG 1 cut(s) 892
AciI CCGC 5 cut(s) 890, 892, 2301, 2863, 3322
AclI AACGTT 1 cut(s) 1701
AclWI GGATC 9 cut(s) 548, 561, 806, 1007, 1020, 1847, 1886, 3147, 3578
AcoI YGGCCR 1 cut(s) 1949
AcsI RAATTY 9 cut(s) 201, 409, 444, 575, 680, 1444, 1459, 2754, 3036
AcuI CTGAAG 4 cut(s) 929, 1317, 1659, 3368
AcyI GRCGYC 1 cut(s) 2764
AdeI CACNNNGTG 2 cut(s) 1901, 2099
AfaI GTAC 5 cut(s) 1580, 2388, 3023, 3452, 3638
AflIII ACRYGT 1 cut(s) 2845
AhdI GACNNNNNGTC 1 cut(s) 3016
AjuI GAANNNNNNNTTGG 6 cut(s) 156, 188, 380, 412, 1657, 1689
AleI CACNNNNGTG 1 cut(s) 2174
AloI GAACNNNNNNTCC 2 cut(s) 474, 506
Alw21I GWGCWC 3 cut(s) 243, 3197, 3346
Alw26I GTCTC 4 cut(s) 58, 228, 2126, 3040
AlwI GGATC 9 cut(s) 548, 561, 806, 1007, 1020, 1847, 1886, 3147, 3578
AlwNI CAGNNNCTG 3 cut(s) 332, 1352, 2161
Ama87I CYCGRG 2 cut(s) 55, 320
ApaI GGGCCC 1 cut(s) 404
ApoI RAATTY 9 cut(s) 201, 409, 444, 575, 680, 1444, 1459, 2754, 3036
ArsI GACNNNNNNTTYG 4 cut(s) 1109, 1141, 2657, 2689
Asp700I GAANNNNTTC 1 cut(s) 3356
Asp718I GGTACC 1 cut(s) 3450
AspA2I CCTAGG 1 cut(s) 971
AspLEI GCGC 2 cut(s) 357, 3585
AspS9I GGNCC 8 cut(s) 210, 400, 401, 524, 1539, 1615, 2665, 3512
AsuC2I CCSGG 1 cut(s) 1199
AsuHPI GGTGA 8 cut(s) 729, 1324, 1834, 1973, 2824, 3469, 3490, 3538
AvaI CYCGRG 2 cut(s) 55, 320
AvaII GGWCC 3 cut(s) 210, 1615, 2665
AvrII CCTAGG 1 cut(s) 971
BaeGI GKGCMC 1 cut(s) 404
BalI TGGCCA 1 cut(s) 1951
BamHI GGATCC 2 cut(s) 553, 1012
BanI GGYRCC 1 cut(s) 3450
BanII GRGCYC 2 cut(s) 243, 404
BbsI GAAGAC 2 cut(s) 1331, 1647
Bbv12I GWGCWC 3 cut(s) 243, 3197, 3346
BceAI ACGGC 2 cut(s) 1135, 3570
BciVI GTATCC 1 cut(s) 3376
BclI TGATCA 4 cut(s) 775, 2467, 3205, 3409
BcnI CCSGG 1 cut(s) 1199
BcoDI GTCTC 4 cut(s) 58, 228, 2126, 3040
BfaI CTAG 9 cut(s) 497, 843, 972, 1719, 1989, 2043, 2460, 2657, 2903
BfmI CTRYAG 6 cut(s) 252, 2223, 2742, 3159, 3212, 3314
BfoI RGCGCY 2 cut(s) 358, 3586
BfuAI ACCTGC 1 cut(s) 2234
BfuI GTATCC 1 cut(s) 3376
BglII AGATCT 1 cut(s) 145
BlnI CCTAGG 1 cut(s) 971
BlpI GCTNAGC 2 cut(s) 903, 2208
BmcAI AGTACT 1 cut(s) 1580
Bme18I GGWCC 3 cut(s) 210, 1615, 2665
BmeRI GACNNNNNGTC 1 cut(s) 3016
BmeT110I CYCGRG 2 cut(s) 55, 320
BmgT120I GGNCC 8 cut(s) 210, 400, 401, 524, 1539, 1615, 2665, 3512
BmrI ACTGGG 2 cut(s) 2952, 3111
BmsI GCATC 8 cut(s) 334, 1198, 2148, 2207, 2557, 3058, 3166, 3449
BmuI ACTGGG 2 cut(s) 2952, 3111
BpiI GAAGAC 2 cut(s) 1331, 1647
BpmI CTGGAG 2 cut(s) 201, 3164
Bpu1102I GCTNAGC 2 cut(s) 903, 2208
BpuEI CTTGAG 4 cut(s) 87, 356, 494, 2411
BpuMI CCSGG 1 cut(s) 1199
BsaAI YACGTR 1 cut(s) 2708
BsaBI GATNNNNATC 5 cut(s) 774, 2481, 2691, 3138, 3575
BsaHI GRCGYC 1 cut(s) 2764
BsaJI CCNNGG 8 cut(s) 740, 815, 890, 971, 1773, 2573, 2824, 3514
BsaXI ACNNNNNCTCC 4 cut(s) 3176, 3206, 3510, 3540
Bse1I ACTGG 5 cut(s) 1108, 2401, 2947, 3016, 3117
Bse3DI GCAATG 3 cut(s) 1863, 2081, 2845
Bse8I GATNNNNATC 5 cut(s) 774, 2481, 2691, 3138, 3575
BseDI CCNNGG 8 cut(s) 740, 815, 890, 971, 1773, 2573, 2824, 3514
BseJI GATNNNNATC 5 cut(s) 774, 2481, 2691, 3138, 3575
BseMI GCAATG 3 cut(s) 1863, 2081, 2845
BseMII CTCAG 9 cut(s) 396, 1127, 1238, 1523, 1601, 2199, 2264, 2624, 3090
BseNI ACTGG 5 cut(s) 1108, 2401, 2947, 3016, 3117
BseRI GAGGAG 3 cut(s) 445, 448, 3266
BseSI GKGCMC 1 cut(s) 404
BseYI CCCAGC 1 cut(s) 2718
BsgI GTGCAG 1 cut(s) 2262
Bsh1236I CGCG 1 cut(s) 892
BshNI GGYRCC 1 cut(s) 3450
BsiHKAI GWGCWC 3 cut(s) 243, 3197, 3346
BsiHKCI CYCGRG 2 cut(s) 55, 320
BsiSI CCGG 2 cut(s) 1199, 1972
BslFI GGGAC 4 cut(s) 580, 1628, 2328, 2678
BsmAI GTCTC 4 cut(s) 58, 228, 2126, 3040
BsmFI GGGAC 4 cut(s) 580, 1628, 2328, 2678
BsmI GAATGC 2 cut(s) 1437, 3331
BsoBI CYCGRG 2 cut(s) 55, 320
Bsp120I GGGCCC 1 cut(s) 400
Bsp1286I GDGCHC 4 cut(s) 243, 404, 3197, 3346
Bsp1720I GCTNAGC 2 cut(s) 903, 2208
BspACI CCGC 5 cut(s) 890, 892, 2301, 2863, 3322
BspCNI CTCAG 9 cut(s) 397, 1128, 1239, 1522, 1602, 2200, 2265, 2623, 3091
BspFNI CGCG 1 cut(s) 892
BspMAI CTGCAG 5 cut(s) 256, 2227, 2746, 3163, 3318
BspMI ACCTGC 1 cut(s) 2234
BspPI GGATC 9 cut(s) 548, 561, 806, 1007, 1020, 1847, 1886, 3147, 3578
BspQI GCTCTTC 1 cut(s) 2708
BspT107I GGYRCC 1 cut(s) 3450
BsrBI CCGCTC 1 cut(s) 3322
BsrDI GCAATG 3 cut(s) 1863, 2081, 2845
BsrI ACTGG 5 cut(s) 1108, 2401, 2947, 3016, 3117
BssECI CCNNGG 8 cut(s) 740, 815, 890, 971, 1773, 2573, 2824, 3514
BssNAI GTATAC 3 cut(s) 1658, 2294, 3291
BssNI GRCGYC 1 cut(s) 2764
BssT1I CCWWGG 3 cut(s) 740, 815, 971
Bst1107I GTATAC 3 cut(s) 1658, 2294, 3291
Bst4CI ACNGT 6 cut(s) 94, 1619, 1756, 2441, 3231, 3341
Bst6I CTCTTC 5 cut(s) 231, 583, 2708, 3100, 3243
BstACI GRCGYC 1 cut(s) 2764
BstAPI GCANNNNNTGC 4 cut(s) 1712, 2204, 2567, 3063
BstBAI YACGTR 1 cut(s) 2708
BstC8I GCNNGC 1 cut(s) 729
BstDSI CCRYGG 1 cut(s) 890
BstEII GGTNACC 1 cut(s) 1411
BstENI CCTNNNNNAGG 2 cut(s) 1216, 2613
BstFNI CGCG 1 cut(s) 892
BstH2I RGCGCY 2 cut(s) 358, 3586
BstHHI GCGC 2 cut(s) 357, 3585
BstMAI GTCTC 4 cut(s) 58, 228, 2126, 3040
BstNSI RCATGY 6 cut(s) 299, 573, 2284, 2849, 2962, 3272
BstPI GGTNACC 1 cut(s) 1411
BstSFI CTRYAG 6 cut(s) 252, 2223, 2742, 3159, 3212, 3314
BstSLI GKGCMC 1 cut(s) 404
BstUI CGCG 1 cut(s) 892
BstV2I GAAGAC 2 cut(s) 1331, 1647
BstX2I RGATCY 5 cut(s) 145, 553, 1012, 1891, 3139
BstXI CCANNNNNNTGG 1 cut(s) 1282
BstYI RGATCY 5 cut(s) 145, 553, 1012, 1891, 3139
BstZ17I GTATAC 3 cut(s) 1658, 2294, 3291
BsuI GTATCC 1 cut(s) 3376
BtgI CCRYGG 1 cut(s) 890
BtgZI GCGATG 2 cut(s) 2412, 3081
BtsI GCAGTG 2 cut(s) 2193, 2872
BveI ACCTGC 1 cut(s) 2234
Cac8I GCNNGC 1 cut(s) 729
CaiI CAGNNNCTG 3 cut(s) 332, 1352, 2161
CfoI GCGC 2 cut(s) 357, 3585
Cfr13I GGNCC 8 cut(s) 210, 400, 401, 524, 1539, 1615, 2665, 3512
Cfr42I CCGCGG 1 cut(s) 893
CseI GACGC 2 cut(s) 458, 2753
CsiI ACCWGGT 1 cut(s) 3453
Csp6I GTAC 5 cut(s) 1579, 2387, 3022, 3451, 3637
CspCI CAANNNNNGTGG 2 cut(s) 3455, 3490
CviQI GTAC 5 cut(s) 1579, 2387, 3022, 3451, 3637
DraI TTTAAA 3 cut(s) 610, 685, 1098
DraIII CACNNNGTG 2 cut(s) 1901, 2099
DriI GACNNNNNGTC 1 cut(s) 3016
EaeI YGGCCR 1 cut(s) 1949
Eam1104I CTCTTC 5 cut(s) 231, 583, 2708, 3100, 3243
Eam1105I GACNNNNNGTC 1 cut(s) 3016
EarI CTCTTC 5 cut(s) 231, 583, 2708, 3100, 3243
Ecl136II GAGCTC 1 cut(s) 241
Eco130I CCWWGG 3 cut(s) 740, 815, 971
Eco147I AGGCCT 2 cut(s) 1987, 2626
Eco24I GRGCYC 2 cut(s) 243, 404
Eco47I GGWCC 3 cut(s) 210, 1615, 2665
Eco53kI GAGCTC 1 cut(s) 241
Eco57I CTGAAG 4 cut(s) 929, 1317, 1659, 3368
Eco88I CYCGRG 2 cut(s) 55, 320
Eco91I GGTNACC 1 cut(s) 1411
EcoICRI GAGCTC 1 cut(s) 241
EcoNI CCTNNNNNAGG 2 cut(s) 1216, 2613
EcoO109I RGGNCCY 2 cut(s) 401, 2665
EcoO65I GGTNACC 1 cut(s) 1411
EcoRI GAATTC 2 cut(s) 409, 1459
EcoT14I CCWWGG 3 cut(s) 740, 815, 971
EcoT22I ATGCAT 1 cut(s) 301
EcoT38I GRGCYC 2 cut(s) 243, 404
ErhI CCWWGG 3 cut(s) 740, 815, 971
FaqI GGGAC 4 cut(s) 580, 1628, 2328, 2678
FauNDI CATATG 1 cut(s) 1747
FbaI TGATCA 4 cut(s) 775, 2467, 3205, 3409
FblI GTMKAC 7 cut(s) 153, 1657, 1742, 2293, 2420, 3029, 3290
FriOI GRGCYC 2 cut(s) 243, 404
FspBI CTAG 9 cut(s) 497, 843, 972, 1719, 1989, 2043, 2460, 2657, 2903
GlaI GCGC 2 cut(s) 356, 3584
GsaI CCCAGC 1 cut(s) 2722
GsuI CTGGAG 2 cut(s) 201, 3164
HaeII RGCGCY 2 cut(s) 358, 3586
HapII CCGG 2 cut(s) 1199, 1972
HgaI GACGC 2 cut(s) 458, 2753
HhaI GCGC 2 cut(s) 357, 3585
Hin1I GRCGYC 1 cut(s) 2764
Hin6I GCGC 2 cut(s) 355, 3583
HinP1I GCGC 2 cut(s) 355, 3583
HincII GTYRAC 3 cut(s) 362, 2286, 2956
HindII GTYRAC 3 cut(s) 362, 2286, 2956
HindIII AAGCTT 3 cut(s) 1259, 2640, 3535
HpaI GTTAAC 1 cut(s) 2956
HpaII CCGG 2 cut(s) 1199, 1972
HphI GGTGA 8 cut(s) 729, 1324, 1834, 1973, 2824, 3469, 3490, 3538
HpyAV CCTTC 9 cut(s) 72, 711, 907, 1421, 1477, 1546, 1603, 2355, 2540
HpyCH4III ACNGT 6 cut(s) 94, 1619, 1756, 2441, 3231, 3341
HpyCH4IV ACGT 2 cut(s) 1701, 2707
HpySE526I ACGT 2 cut(s) 1701, 2707
Hsp92I GRCGYC 1 cut(s) 2764
HspAI GCGC 2 cut(s) 355, 3583
KpnI GGTACC 1 cut(s) 3454
Ksp22I TGATCA 4 cut(s) 775, 2467, 3205, 3409
KspAI GTTAAC 1 cut(s) 2956
KspI CCGCGG 1 cut(s) 893
LguI GCTCTTC 1 cut(s) 2708
LmnI GCTCC 6 cut(s) 1477, 2011, 2828, 2876, 3310, 3319
LweI GCATC 8 cut(s) 334, 1198, 2148, 2207, 2557, 3058, 3166, 3449
MabI ACCWGGT 1 cut(s) 3453
MaeI CTAG 9 cut(s) 497, 843, 972, 1719, 1989, 2043, 2460, 2657, 2903
MaeII ACGT 2 cut(s) 1701, 2707
MbiI CCGCTC 1 cut(s) 3322
MflI RGATCY 5 cut(s) 145, 553, 1012, 1891, 3139
MhlI GDGCHC 4 cut(s) 243, 404, 3197, 3346
MlsI TGGCCA 1 cut(s) 1951
MluNI TGGCCA 1 cut(s) 1951
MlyI GAGTC 6 cut(s) 230, 332, 2015, 3042, 3404, 3599
MmeI TCCRAC 4 cut(s) 780, 990, 1386, 1989
Mox20I TGGCCA 1 cut(s) 1951
Mph1103I ATGCAT 1 cut(s) 301
MroXI GAANNNNTTC 1 cut(s) 3356
MscI TGGCCA 1 cut(s) 1951
MslI CAYNNNNRTG 5 cut(s) 1284, 1547, 2174, 2589, 2634
Msp20I TGGCCA 1 cut(s) 1951
MspA1I CMGCKG 2 cut(s) 892, 3554
MspI CCGG 2 cut(s) 1199, 1972
Mva1269I GAATGC 2 cut(s) 1437, 3331
MvnI CGCG 1 cut(s) 892
NciI CCSGG 1 cut(s) 1199
NdeI CATATG 1 cut(s) 1747
NmuCI GTSAC 9 cut(s) 328, 2651, 2677, 2770, 3119, 3259, 3396, 3457, 3486
NsiI ATGCAT 1 cut(s) 301
NspI RCATGY 6 cut(s) 299, 573, 2284, 2849, 2962, 3272
OliI CACNNNNGTG 1 cut(s) 2174
PceI AGGCCT 2 cut(s) 1987, 2626
PciI ACATGT 1 cut(s) 2845
PciSI GCTCTTC 1 cut(s) 2708
PcsI WCGNNNNNNNCGW 1 cut(s) 2015
PctI GAATGC 2 cut(s) 1437, 3331
PdmI GAANNNNTTC 1 cut(s) 3356
PfeI GAWTC 6 cut(s) 8, 976, 1270, 1665, 2521, 2551
PflMI CCANNNNNTGG 1 cut(s) 533
PfoI TCCNGGA 1 cut(s) 2536
PleI GAGTC 6 cut(s) 229, 331, 2014, 3042, 3403, 3599
PpsI GAGTC 6 cut(s) 229, 331, 2014, 3042, 3403, 3599
Ppu21I YACGTR 1 cut(s) 2708
PpuMI RGGWCCY 1 cut(s) 2665
PscI ACATGT 1 cut(s) 2845
PsiI TTATAA 1 cut(s) 2078
Psp124BI GAGCTC 1 cut(s) 243
Psp1406I AACGTT 1 cut(s) 1701
Psp5II RGGWCCY 1 cut(s) 2665
PspEI GGTNACC 1 cut(s) 1411
PspFI CCCAGC 1 cut(s) 2718
PspOMI GGGCCC 1 cut(s) 400
PspPI GGNCC 8 cut(s) 210, 400, 401, 524, 1539, 1615, 2665, 3512
PspPPI RGGWCCY 1 cut(s) 2665
PstI CTGCAG 5 cut(s) 256, 2227, 2746, 3163, 3318
PstNI CAGNNNCTG 3 cut(s) 332, 1352, 2161
PsuI RGATCY 5 cut(s) 145, 553, 1012, 1891, 3139
PvuII CAGCTG 1 cut(s) 3554
RsaI GTAC 5 cut(s) 1580, 2388, 3023, 3452, 3638
RsaNI GTAC 5 cut(s) 1579, 2387, 3022, 3451, 3637
RseI CAYNNNNRTG 5 cut(s) 1284, 1547, 2174, 2589, 2634
SacI GAGCTC 1 cut(s) 243
SacII CCGCGG 1 cut(s) 893
SapI GCTCTTC 1 cut(s) 2708
Sau96I GGNCC 8 cut(s) 210, 400, 401, 524, 1539, 1615, 2665, 3512
ScaI AGTACT 1 cut(s) 1580
SchI GAGTC 6 cut(s) 230, 332, 2015, 3042, 3404, 3599
SduI GDGCHC 4 cut(s) 243, 404, 3197, 3346
SexAI ACCWGGT 1 cut(s) 3453
SfaNI GCATC 8 cut(s) 334, 1198, 2148, 2207, 2557, 3058, 3166, 3449
SfcI CTRYAG 6 cut(s) 252, 2223, 2742, 3159, 3212, 3314
Sfr303I CCGCGG 1 cut(s) 893
SgrBI CCGCGG 1 cut(s) 893
SinI GGWCC 3 cut(s) 210, 1615, 2665
SmiMI CAYNNNNRTG 5 cut(s) 1284, 1547, 2174, 2589, 2634
SmlI CTYRAG 4 cut(s) 102, 371, 473, 2390
SmoI CTYRAG 4 cut(s) 102, 371, 473, 2390
SseBI AGGCCT 2 cut(s) 1987, 2626
SsiI CCGC 5 cut(s) 890, 892, 2301, 2863, 3322
SspI AATATT 3 cut(s) 1045, 2115, 3601
SspMI CTAG 9 cut(s) 497, 843, 972, 1719, 1989, 2043, 2460, 2657, 2903
SstI GAGCTC 1 cut(s) 243
StuI AGGCCT 2 cut(s) 1987, 2626
StyI CCWWGG 3 cut(s) 740, 815, 971
TaaI ACNGT 6 cut(s) 94, 1619, 1756, 2441, 3231, 3341
TaiI ACGT 2 cut(s) 1704, 2710
TaqI TCGA 8 cut(s) 13, 41, 754, 956, 2790, 2883, 3372, 3492
TatI WGTACW 1 cut(s) 1578
TauI GCSGC 2 cut(s) 895, 2303
TfiI GAWTC 6 cut(s) 8, 976, 1270, 1665, 2521, 2551
TseFI GTSAC 9 cut(s) 328, 2651, 2677, 2770, 3119, 3259, 3396, 3457, 3486
Tsp45I GTSAC 9 cut(s) 328, 2651, 2677, 2770, 3119, 3259, 3396, 3457, 3486
TspGWI ACGGA 1 cut(s) 1935
Van91I CCANNNNNTGG 1 cut(s) 533
VpaK11BI GGWCC 3 cut(s) 210, 1615, 2665
XagI CCTNNNNNAGG 2 cut(s) 1216, 2613
XapI RAATTY 9 cut(s) 201, 409, 444, 575, 680, 1444, 1459, 2754, 3036
XceI RCATGY 6 cut(s) 299, 573, 2284, 2849, 2962, 3272
XmaJI CCTAGG 1 cut(s) 971
XmiI GTMKAC 7 cut(s) 153, 1657, 1742, 2293, 2420, 3029, 3290
XmnI GAANNNNTTC 1 cut(s) 3356
XspI CTAG 9 cut(s) 497, 843, 972, 1719, 1989, 2043, 2460, 2657, 2903
ZrmI AGTACT 1 cut(s) 1580
Zsp2I ATGCAT 1 cut(s) 301
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.