AT3G50360

calcium-binding protein CML20

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0013400
Physical Location & Seq
Forward (+)
18674137 .. 18675761
1625 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G50360.1

Sequence Viewer

Length: 510 bp
ATGTCGAGTATATACAGAACTGTTTCGAGAAAAGAGAAACCGAGACGTCATCATGGATTGACGACACAGAAGAAGCAAGAGATTAAGGAAGCTTTTGAGCTATTTGACACTGATGGTTCTGGTACCATTGATGCTAAAGAGCTTAATGTTGCTATGAGGGCGCTTGGTTTTGAAATGACGGAAGAGCAAATCAACAAAATGATAGCTGATGTGGATAAAGATGGAAGTGGAGCTATAGATTTTGATGAGTTTGTTCATATGATGACTGCTAAGATTGGTGAAAGAGACACAAAAGAAGAGCTCACTAAAGCATTCCAGATCATTGATCTTGACAAAAATGGGAAGATATCTCCGGATGATATCAAACGCATGGCAAAGGACTTGGGTGAGAATTTCACTGATGCTGAGATACGAGAGATGGTTGAAGAAGCAGACCGAGACCGTGATGGTGAAGTTAACATGGATGAATTCATGAGGATGATGAGGAGAACTGCTTATGGTGGTAACTAG

Protein Analysis

169

Amino Acids

19.41

Weight (kDa)

4.91

Isoelectric Point (pI)

27.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AIF-1 PF21008 22 - 91 8.3e-08 Allograft inflammatory factor 1
EF-hand_7 PF13499 26 - 89 2.1e-18 EF-hand domain pair
EF-hand_1 PF00036 27 - 55 1.8e-08 EF hand domain
EF-hand_6 PF13405 27 - 56 1.8e-08 EF-hand domain
EF-hand_5 PF13202 28 - 49 3.5e-06 EF hand
EF-hand_9 PF14658 30 - 91 6.2e-07 EF-hand domain
EF-hand_8 PF13833 41 - 89 2.6e-07 EF-hand domain pair
EF-hand_1 PF00036 64 - 90 2.1e-08 EF hand domain
EF-hand_7 PF13499 98 - 162 3.2e-16 EF-hand domain pair
EF-hand_6 PF13405 100 - 129 6.5e-06 EF-hand domain
EF-hand_8 PF13833 113 - 163 1.3e-11 EF-hand domain pair
EF-hand_1 PF00036 136 - 163 5.1e-07 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0013400)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G50360 AT4G37010 AT4G37010
fragaria_vesca FvH4_1g15980
malus_domestica MD02G1171500.v1.1 MD15G1283300.v1.1
prunus_persica Prupe.7G133900_v2.0.a1 Prupe.7G133900_v2.0.a1
pyrus_communis pycom02g13820 pycom15g24650
rosa_chinensis RchiOBHm_Chr2g0105581
rosa_laevigata RLG00000017438
rosa_rugosa Rorug02G0128400
rosa_samantha Rh2AG180000 Rh2BG189100 Rh2CG184300 Rh2DG185800
rosa_wichuraiana Rw2G014080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 49
Acc65I GGTACC 1 cut(s) 122
AccB1I GGYRCC 1 cut(s) 122
AccIII TCCGGA 1 cut(s) 352
AcsI RAATTY 2 cut(s) 391, 467
AcyI GRCGYC 1 cut(s) 46
AfaI GTAC 1 cut(s) 124
AgsI TTSAA 2 cut(s) 173, 425
AluBI AGCT 6 cut(s) 92, 100, 142, 206, 233, 301
AluI AGCT 6 cut(s) 92, 100, 142, 206, 233, 301
Alw21I GWGCWC 1 cut(s) 303
Alw26I GTCTC 3 cut(s) 37, 279, 432
Aor13HI TCCGGA 1 cut(s) 352
ApoI RAATTY 2 cut(s) 391, 467
Asp700I GAANNNNTTC 1 cut(s) 22
Asp718I GGTACC 1 cut(s) 122
AspLEI GCGC 1 cut(s) 163
AsuHPI GGTGA 3 cut(s) 290, 398, 461
BanI GGYRCC 1 cut(s) 122
BanII GRGCYC 1 cut(s) 303
Bbv12I GWGCWC 1 cut(s) 303
BccI CCATC 4 cut(s) 107, 215, 412, 440
BcoDI GTCTC 3 cut(s) 37, 279, 432
BfaI CTAG 1 cut(s) 508
BfmI CTRYAG 1 cut(s) 234
BfoI RGCGCY 1 cut(s) 164
BmiI GGNNCC 1 cut(s) 124
BmsI GCATC 2 cut(s) 121, 391
BsaHI GRCGYC 1 cut(s) 46
BsaI GGTCTC 1 cut(s) 432
BsaWI WCCGGW 1 cut(s) 352
BseAI TCCGGA 1 cut(s) 352
BseGI GGATG 3 cut(s) 361, 469, 483
BseMII CTCAG 1 cut(s) 396
BseRI GAGGAG 1 cut(s) 499
BshNI GGYRCC 1 cut(s) 122
BsiHKAI GWGCWC 1 cut(s) 303
BsiSI CCGG 1 cut(s) 353
BsmAI GTCTC 3 cut(s) 37, 279, 432
BsmBI CGTCTC 1 cut(s) 37
BsmI GAATGC 1 cut(s) 311
Bso31I GGTCTC 1 cut(s) 432
Bsp1286I GDGCHC 1 cut(s) 303
Bsp13I TCCGGA 1 cut(s) 352
Bsp143I GATC 2 cut(s) 318, 325
BspCNI CTCAG 1 cut(s) 397
BspEI TCCGGA 1 cut(s) 352
BspHI TCATGA 1 cut(s) 471
BspLI GGNNCC 1 cut(s) 124
BspQI GCTCTTC 2 cut(s) 177, 291
BspT107I GGYRCC 1 cut(s) 122
BspTNI GGTCTC 1 cut(s) 432
BssMI GATC 2 cut(s) 318, 325
BssNI GRCGYC 1 cut(s) 46
Bst4CI ACNGT 2 cut(s) 22, 443
Bst6I CTCTTC 2 cut(s) 177, 291
BstACI GRCGYC 1 cut(s) 46
BstDEI CTNAG 2 cut(s) 270, 405
BstF5I GGATG 3 cut(s) 361, 469, 483
BstH2I RGCGCY 1 cut(s) 164
BstHHI GCGC 1 cut(s) 163
BstKTI GATC 2 cut(s) 321, 328
BstMAI GTCTC 3 cut(s) 37, 279, 432
BstMBI GATC 2 cut(s) 318, 325
BstMWI GCNNNNNNNGC 1 cut(s) 158
BstSFI CTRYAG 1 cut(s) 234
BtsCI GGATG 3 cut(s) 361, 469, 483
BtsIMutI CAGTG 2 cut(s) 108, 396
CciI TCATGA 1 cut(s) 471
CfoI GCGC 1 cut(s) 163
Csp6I GTAC 1 cut(s) 123
CviAII CATG 4 cut(s) 53, 370, 460, 472
CviJI RGCY 6 cut(s) 92, 100, 142, 206, 233, 301
CviKI_1 RGCY 6 cut(s) 92, 100, 142, 206, 233, 301
CviQI GTAC 1 cut(s) 123
DdeI CTNAG 2 cut(s) 270, 405
DpnI GATC 2 cut(s) 320, 327
DpnII GATC 2 cut(s) 318, 325
Eam1104I CTCTTC 2 cut(s) 177, 291
EarI CTCTTC 2 cut(s) 177, 291
Ecl136II GAGCTC 1 cut(s) 301
Eco24I GRGCYC 1 cut(s) 303
Eco31I GGTCTC 1 cut(s) 432
Eco32I GATATC 2 cut(s) 348, 361
Eco53kI GAGCTC 1 cut(s) 301
EcoICRI GAGCTC 1 cut(s) 301
EcoRI GAATTC 1 cut(s) 467
EcoRV GATATC 2 cut(s) 348, 361
EcoT38I GRGCYC 1 cut(s) 303
Esp3I CGTCTC 1 cut(s) 37
FaeI CATG 4 cut(s) 56, 373, 463, 475
FatI CATG 4 cut(s) 52, 369, 459, 471
FauNDI CATATG 1 cut(s) 258
FokI GGATG 3 cut(s) 368, 476, 490
FriOI GRGCYC 1 cut(s) 303
FspBI CTAG 1 cut(s) 508
GlaI GCGC 1 cut(s) 162
HaeII RGCGCY 1 cut(s) 164
HapII CCGG 1 cut(s) 353
HhaI GCGC 1 cut(s) 163
Hin1I GRCGYC 1 cut(s) 46
Hin1II CATG 4 cut(s) 56, 373, 463, 475
Hin6I GCGC 1 cut(s) 161
HinP1I GCGC 1 cut(s) 161
HincII GTYRAC 1 cut(s) 457
HindII GTYRAC 1 cut(s) 457
HindIII AAGCTT 1 cut(s) 90
HpaI GTTAAC 1 cut(s) 457
HpaII CCGG 1 cut(s) 353
HphI GGTGA 3 cut(s) 290, 398, 461
Hpy166II GTNNAC 1 cut(s) 457
Hpy188III TCNNGA 5 cut(s) 27, 316, 329, 353, 472
Hpy8I GTNNAC 1 cut(s) 457
HpyCH4III ACNGT 2 cut(s) 22, 443
HpyCH4IV ACGT 1 cut(s) 46
HpyF10VI GCNNNNNNNGC 1 cut(s) 158
HpyF3I CTNAG 2 cut(s) 270, 405
HpySE526I ACGT 1 cut(s) 46
Hsp92I GRCGYC 1 cut(s) 46
Hsp92II CATG 4 cut(s) 56, 373, 463, 475
HspAI GCGC 1 cut(s) 161
Kpn2I TCCGGA 1 cut(s) 352
KpnI GGTACC 1 cut(s) 126
KspAI GTTAAC 1 cut(s) 457
Kzo9I GATC 2 cut(s) 318, 325
LguI GCTCTTC 2 cut(s) 177, 291
LmnI GCTCC 1 cut(s) 230
LpnPI CCDG 3 cut(s) 105, 329, 366
LweI GCATC 2 cut(s) 121, 391
MaeI CTAG 1 cut(s) 508
MaeII ACGT 1 cut(s) 46
MaeIII GTNAC 1 cut(s) 503
MalI GATC 2 cut(s) 320, 327
MboI GATC 2 cut(s) 318, 325
MboII GAAGA 5 cut(s) 82, 194, 308, 355, 437
MhlI GDGCHC 1 cut(s) 303
MluCI AATT 2 cut(s) 391, 467
MnlI CCTC 3 cut(s) 150, 468, 477
MroI TCCGGA 1 cut(s) 352
MroXI GAANNNNTTC 1 cut(s) 22
MseI TTAA 3 cut(s) 84, 144, 456
MslI CAYNNNNRTG 1 cut(s) 476
MspI CCGG 1 cut(s) 353
Mva1269I GAATGC 1 cut(s) 311
MwoI GCNNNNNNNGC 1 cut(s) 158
NdeI CATATG 1 cut(s) 258
NdeII GATC 2 cut(s) 318, 325
NlaIII CATG 4 cut(s) 56, 373, 463, 475
NlaIV GGNNCC 1 cut(s) 124
PagI TCATGA 1 cut(s) 471
PciSI GCTCTTC 2 cut(s) 177, 291
PctI GAATGC 1 cut(s) 311
PdmI GAANNNNTTC 1 cut(s) 22
Psp124BI GAGCTC 1 cut(s) 303
PspN4I GGNNCC 1 cut(s) 124
RsaI GTAC 1 cut(s) 124
RsaNI GTAC 1 cut(s) 123
RseI CAYNNNNRTG 1 cut(s) 476
SacI GAGCTC 1 cut(s) 303
SapI GCTCTTC 2 cut(s) 177, 291
SaqAI TTAA 3 cut(s) 84, 144, 456
Sau3AI GATC 2 cut(s) 318, 325
SduI GDGCHC 1 cut(s) 303
SetI ASST 7 cut(s) 49, 94, 102, 144, 208, 235, 303
SfaNI GCATC 2 cut(s) 121, 391
SfcI CTRYAG 1 cut(s) 234
SmiMI CAYNNNNRTG 1 cut(s) 476
Sse9I AATT 2 cut(s) 391, 467
SspMI CTAG 1 cut(s) 508
SstI GAGCTC 1 cut(s) 303
TaaI ACNGT 2 cut(s) 22, 443
TaiI ACGT 1 cut(s) 49
TaqI TCGA 2 cut(s) 5, 26
TaqII GACCGA 1 cut(s) 450
TasI AATT 2 cut(s) 391, 467
Tru1I TTAA 3 cut(s) 84, 144, 456
Tru9I TTAA 3 cut(s) 84, 144, 456
TscAI CASTG 2 cut(s) 115, 403
TspDTI ATGAA 3 cut(s) 245, 460, 480
TspGWI ACGGA 1 cut(s) 194
TspRI CASTG 2 cut(s) 115, 403
XapI RAATTY 2 cut(s) 391, 467
XmnI GAANNNNTTC 1 cut(s) 22
XspI CTAG 1 cut(s) 508
ZraI GACGTC 1 cut(s) 47
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.