MD15G1283300.v1.1

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0013400
Physical Location & Seq
Reverse (-)
25610148 .. 25612848
2701 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1283300.v1.1.491

Sequence Viewer

Length: 510 bp
ATGGCAAGCCTTTACAGAGGGGCATCTAGGAAGGAGAAACCCAGAGGGAGACATCATGGGTTAACTCAACAAAAGAGGCAAGAGATTAAGGAGGCATTCGAGTTATTCGACACCGATGGCTCAGGCACCATTGATGCCAAGGAGTTGAATGTTGCCATGAGGGCTCTCGGTTTTGAGATGACTGAAGAGCAAATTAATCAAATGATTGCAGATGTTGACAAGGATGGCAGTGGTGCTATCGACTTTGATGAGTTTGCACACATGATGACAGCCAAAATTGGAGAACGGGACACAAAGGAGGAACTCATGAAAGCATTCCAGCTCATCGACCTAGATCGCAATGGAAAGATATCTGCTGCAGACATTAAAAACATTGCAAAGGATCTGGGTGAGAACTTCACAGACAGAGAGATCCAAGAGATGATTGAGGAAGCAGACCGGGATCGTGATGGAGAAGTCAATGCTGATGAGTTTATCAGGATGATGAAGAGAACAGCATACGGGTATTAG

Protein Analysis

170

Amino Acids

19.29

Weight (kDa)

4.83

Isoelectric Point (pI)

17.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AIF-1 PF21008 23 - 92 2.3e-06 Allograft inflammatory factor 1
EF-hand_7 PF13499 27 - 90 2.3e-18 EF-hand domain pair
EF-hand_1 PF00036 28 - 56 1.8e-08 EF hand domain
EF-hand_6 PF13405 28 - 57 1.8e-08 EF-hand domain
EF-hand_5 PF13202 29 - 50 3.5e-06 EF hand
EF-hand_9 PF14658 31 - 92 8.1e-07 EF-hand domain
EF-hand_8 PF13833 42 - 90 5e-07 EF-hand domain pair
EF-hand_1 PF00036 65 - 91 8.3e-08 EF hand domain
EF-hand_7 PF13499 99 - 163 4.9e-16 EF-hand domain pair
EF-hand_6 PF13405 101 - 130 5.8e-06 EF-hand domain
EF-hand_8 PF13833 114 - 164 7.6e-12 EF-hand domain pair
EF-hand_1 PF00036 137 - 164 2.6e-08 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0013400)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G50360 AT4G37010 AT4G37010
fragaria_vesca FvH4_1g15980
malus_domestica MD02G1171500.v1.1 MD15G1283300.v1.1
prunus_persica Prupe.7G133900_v2.0.a1 Prupe.7G133900_v2.0.a1
pyrus_communis pycom02g13820 pycom15g24650
rosa_chinensis RchiOBHm_Chr2g0105581
rosa_laevigata RLG00000017438
rosa_rugosa Rorug02G0128400
rosa_samantha Rh2AG180000 Rh2BG189100 Rh2CG184300 Rh2DG185800
rosa_wichuraiana Rw2G014080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 125
AclWI GGATC 3 cut(s) 390, 406, 450
AcuI CTGAAG 1 cut(s) 204
AgsI TTSAA 1 cut(s) 148
AluBI AGCT 1 cut(s) 322
AluI AGCT 1 cut(s) 322
Alw26I GTCTC 1 cut(s) 43
AlwI GGATC 3 cut(s) 390, 406, 450
ApeKI GCWGC 1 cut(s) 356
AseI ATTAAT 1 cut(s) 195
Asp700I GAANNNNTTC 1 cut(s) 314
AsuC2I CCSGG 1 cut(s) 440
AsuHPI GGTGA 1 cut(s) 401
BanI GGYRCC 1 cut(s) 125
BanII GRGCYC 1 cut(s) 166
BbvI GCAGC 1 cut(s) 343
BccI CCATC 3 cut(s) 110, 218, 443
BcnI CCSGG 1 cut(s) 440
BcoDI GTCTC 1 cut(s) 43
BfaI CTAG 2 cut(s) 27, 332
BfmI CTRYAG 1 cut(s) 357
BglI GCCNNNNNGGC 1 cut(s) 161
BisI GCNGC 1 cut(s) 357
BlsI GCNGC 1 cut(s) 358
Bme1390I CCNGG 1 cut(s) 440
BmiI GGNNCC 1 cut(s) 127
BmrFI CCNGG 1 cut(s) 440
BmsI GCATC 2 cut(s) 32, 124
Bpu10I CCTNAGC 1 cut(s) 121
BpuMI CCSGG 1 cut(s) 440
BsaJI CCNNGG 1 cut(s) 138
Bse3DI GCAATG 2 cut(s) 346, 372
BseDI CCNNGG 1 cut(s) 138
BseGI GGATG 2 cut(s) 229, 486
BseMI GCAATG 2 cut(s) 346, 372
BseMII CTCAG 1 cut(s) 135
BseXI GCAGC 1 cut(s) 343
BshNI GGYRCC 1 cut(s) 125
BsiSI CCGG 1 cut(s) 439
BslFI GGGAC 1 cut(s) 302
BsmAI GTCTC 1 cut(s) 43
BsmFI GGGAC 1 cut(s) 302
BsmI GAATGC 2 cut(s) 95, 314
Bsp1286I GDGCHC 1 cut(s) 166
Bsp143I GATC 4 cut(s) 334, 382, 411, 442
BspCNI CTCAG 1 cut(s) 134
BspHI TCATGA 1 cut(s) 306
BspLI GGNNCC 1 cut(s) 127
BspMAI CTGCAG 1 cut(s) 361
BspPI GGATC 3 cut(s) 390, 406, 450
BspQI GCTCTTC 1 cut(s) 180
BspT107I GGYRCC 1 cut(s) 125
BsrDI GCAATG 2 cut(s) 346, 372
BssECI CCNNGG 1 cut(s) 138
BssMI GATC 4 cut(s) 334, 382, 411, 442
BssT1I CCWWGG 1 cut(s) 138
Bst6I CTCTTC 2 cut(s) 180, 482
BstC8I GCNNGC 1 cut(s) 7
BstDEI CTNAG 1 cut(s) 121
BstF5I GGATG 2 cut(s) 229, 486
BstKTI GATC 4 cut(s) 337, 385, 414, 445
BstMAI GTCTC 1 cut(s) 43
BstMBI GATC 4 cut(s) 334, 382, 411, 442
BstMWI GCNNNNNNNGC 1 cut(s) 161
BstSCI CCNGG 1 cut(s) 438
BstSFI CTRYAG 1 cut(s) 357
BstV1I GCAGC 1 cut(s) 343
BstX2I RGATCY 2 cut(s) 382, 411
BstYI RGATCY 2 cut(s) 382, 411
BtsCI GGATG 2 cut(s) 229, 486
BtsI GCAGTG 1 cut(s) 235
BtsIMutI CAGTG 1 cut(s) 235
Cac8I GCNNGC 1 cut(s) 7
CciI TCATGA 1 cut(s) 306
CviAII CATG 4 cut(s) 56, 157, 262, 307
CviJI RGCY 5 cut(s) 9, 120, 164, 272, 322
CviKI_1 RGCY 5 cut(s) 9, 120, 164, 272, 322
DdeI CTNAG 1 cut(s) 121
DpnI GATC 4 cut(s) 336, 384, 413, 444
DpnII GATC 4 cut(s) 334, 382, 411, 442
Eam1104I CTCTTC 2 cut(s) 180, 482
EarI CTCTTC 2 cut(s) 180, 482
Eco130I CCWWGG 1 cut(s) 138
Eco24I GRGCYC 1 cut(s) 166
Eco32I GATATC 1 cut(s) 351
Eco57I CTGAAG 1 cut(s) 204
EcoRV GATATC 1 cut(s) 351
EcoT14I CCWWGG 1 cut(s) 138
EcoT38I GRGCYC 1 cut(s) 166
ErhI CCWWGG 1 cut(s) 138
FaeI CATG 4 cut(s) 59, 160, 265, 310
FaiI YATR 5 cut(s) 57, 158, 263, 308, 499
FaqI GGGAC 1 cut(s) 302
FatI CATG 4 cut(s) 55, 156, 261, 306
Fnu4HI GCNGC 1 cut(s) 357
FokI GGATG 2 cut(s) 236, 493
FriOI GRGCYC 1 cut(s) 166
Fsp4HI GCNGC 1 cut(s) 357
FspBI CTAG 2 cut(s) 27, 332
GluI GCNGC 1 cut(s) 357
HapII CCGG 1 cut(s) 439
Hin1II CATG 4 cut(s) 59, 160, 265, 310
HincII GTYRAC 2 cut(s) 63, 217
HindII GTYRAC 2 cut(s) 63, 217
HpaI GTTAAC 1 cut(s) 63
HpaII CCGG 1 cut(s) 439
HphI GGTGA 1 cut(s) 401
Hpy166II GTNNAC 2 cut(s) 63, 217
Hpy188III TCNNGA 3 cut(s) 307, 446, 478
Hpy8I GTNNAC 2 cut(s) 63, 217
HpyAV CCTTC 1 cut(s) 25
HpyCH4V TGCA 4 cut(s) 209, 257, 359, 377
HpyF10VI GCNNNNNNNGC 1 cut(s) 161
HpyF3I CTNAG 1 cut(s) 121
Hsp92II CATG 4 cut(s) 59, 160, 265, 310
KspAI GTTAAC 1 cut(s) 63
Kzo9I GATC 4 cut(s) 334, 382, 411, 442
LguI GCTCTTC 1 cut(s) 180
LpnPI CCDG 6 cut(s) 55, 108, 332, 371, 452, 463
Lsp1109I GCAGC 1 cut(s) 343
LweI GCATC 2 cut(s) 32, 124
MaeI CTAG 2 cut(s) 27, 332
MalI GATC 4 cut(s) 336, 384, 413, 444
MboI GATC 4 cut(s) 334, 382, 411, 442
MboII GAAGA 2 cut(s) 197, 499
MflI RGATCY 2 cut(s) 382, 411
MhlI GDGCHC 1 cut(s) 166
MluCI AATT 2 cut(s) 192, 276
MnlI CCTC 7 cut(s) 11, 38, 69, 85, 153, 292, 421
MroXI GAANNNNTTC 1 cut(s) 314
MseI TTAA 4 cut(s) 62, 87, 195, 366
MspI CCGG 1 cut(s) 439
MspR9I CCNGG 1 cut(s) 440
Mva1269I GAATGC 2 cut(s) 95, 314
MwoI GCNNNNNNNGC 1 cut(s) 161
NciI CCSGG 1 cut(s) 440
NdeII GATC 4 cut(s) 334, 382, 411, 442
NlaIII CATG 4 cut(s) 59, 160, 265, 310
NlaIV GGNNCC 1 cut(s) 127
PagI TCATGA 1 cut(s) 306
PciSI GCTCTTC 1 cut(s) 180
PcsI WCGNNNNNNNCGW 1 cut(s) 105
PctI GAATGC 2 cut(s) 95, 314
PdmI GAANNNNTTC 1 cut(s) 314
PkrI GCNGC 1 cut(s) 358
PshBI ATTAAT 1 cut(s) 195
PspN4I GGNNCC 1 cut(s) 127
PstI CTGCAG 1 cut(s) 361
PsuI RGATCY 2 cut(s) 382, 411
SapI GCTCTTC 1 cut(s) 180
SaqAI TTAA 4 cut(s) 62, 87, 195, 366
SatI GCNGC 1 cut(s) 357
Sau3AI GATC 4 cut(s) 334, 382, 411, 442
ScrFI CCNGG 1 cut(s) 440
SduI GDGCHC 1 cut(s) 166
SetI ASST 2 cut(s) 324, 333
SfaNI GCATC 2 cut(s) 32, 124
SfcI CTRYAG 1 cut(s) 357
Sse9I AATT 2 cut(s) 192, 276
SspMI CTAG 2 cut(s) 27, 332
StyD4I CCNGG 1 cut(s) 438
StyI CCWWGG 1 cut(s) 138
TaqI TCGA 4 cut(s) 99, 108, 240, 327
TasI AATT 2 cut(s) 192, 276
Tru1I TTAA 4 cut(s) 62, 87, 195, 366
Tru9I TTAA 4 cut(s) 62, 87, 195, 366
TscAI CASTG 1 cut(s) 235
TseI GCWGC 1 cut(s) 356
TspDTI ATGAA 2 cut(s) 323, 500
TspRI CASTG 1 cut(s) 235
VspI ATTAAT 1 cut(s) 195
XmnI GAANNNNTTC 1 cut(s) 314
XspI CTAG 2 cut(s) 27, 332
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.