MD02G1171500.v1.1

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0013400
Physical Location & Seq
Reverse (-)
14855498 .. 14857440
1943 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1171500.v1.1.491

Sequence Viewer

Length: 510 bp
ATGGCAAGCCTTTACAGAGGGCCATCTAGGAAGGAGAAACCCAGAGGGCGACATCACGGGTTAACTCAACAAAAGAGGCAAGAGATTAAGGAGGCATTCGAGTTATTCGACACCGATGGCTCGGGCACTATTGATGCCAAGGAGCTGAATGTTGCCATGAGGGCTCTCGGTTTTGAGATGACCGAAGAGCAAATTCATCAAATGATTGCAGATGTTGACAAGGATGGCAGTGGTGCTATCGACTTTGATGAGTTTGCTCACATGATGACCGCCAAAATTGGAGAACGGGACACAAAGGAGGAGCTCATGAAAGCATTCCAGCTCATTGACCTAGATCGCAATGGAAAGATATCTGCAGCAGACATTAAGAGCATTGCAAAGGATCTGGGTGAGAACTTCACTGACAGCGAGATCCAAGAGATGATTGAGGAAGCAGACCGAGATCGTGATGGAGAAGTTAATGCTGATGAGTTTATCAGGATGATGAAGAGAACAGCATACGGGTATTAG

Protein Analysis

170

Amino Acids

19.24

Weight (kDa)

4.83

Isoelectric Point (pI)

20.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AIF-1 PF21008 23 - 92 2.3e-06 Allograft inflammatory factor 1
EF-hand_7 PF13499 27 - 90 8.4e-18 EF-hand domain pair
EF-hand_1 PF00036 28 - 56 1.8e-08 EF hand domain
EF-hand_6 PF13405 28 - 57 1.8e-08 EF-hand domain
EF-hand_5 PF13202 29 - 50 3.5e-06 EF hand
EF-hand_9 PF14658 31 - 92 1.1e-06 EF-hand domain
EF-hand_8 PF13833 41 - 90 5e-07 EF-hand domain pair
EF-hand_1 PF00036 65 - 91 8.4e-08 EF hand domain
EF-hand_7 PF13499 99 - 163 1.3e-16 EF-hand domain pair
EF-hand_9 PF14658 105 - 164 3.1e-06 EF-hand domain
EF-hand_8 PF13833 114 - 164 2.2e-13 EF-hand domain pair
EF-hand_1 PF00036 137 - 164 2.6e-08 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0013400)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G50360 AT4G37010 AT4G37010
fragaria_vesca FvH4_1g15980
malus_domestica MD02G1171500.v1.1 MD15G1283300.v1.1
prunus_persica Prupe.7G133900_v2.0.a1 Prupe.7G133900_v2.0.a1
pyrus_communis pycom02g13820 pycom15g24650
rosa_chinensis RchiOBHm_Chr2g0105581
rosa_laevigata RLG00000017438
rosa_rugosa Rorug02G0128400
rosa_samantha Rh2AG180000 Rh2BG189100 Rh2CG184300 Rh2DG185800
rosa_wichuraiana Rw2G014080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 270
AclWI GGATC 2 cut(s) 390, 406
AcsI RAATTY 1 cut(s) 192
AluBI AGCT 3 cut(s) 145, 304, 322
AluI AGCT 3 cut(s) 145, 304, 322
Alw21I GWGCWC 1 cut(s) 306
AlwI GGATC 2 cut(s) 390, 406
Ama87I CYCGRG 1 cut(s) 121
AoxI GGCC 1 cut(s) 20
ApeKI GCWGC 1 cut(s) 356
ApoI RAATTY 1 cut(s) 192
Asp700I GAANNNNTTC 1 cut(s) 314
AspS9I GGNCC 1 cut(s) 20
AsuHPI GGTGA 1 cut(s) 401
AvaI CYCGRG 1 cut(s) 121
BaeGI GKGCMC 1 cut(s) 128
BanII GRGCYC 2 cut(s) 166, 306
Bbv12I GWGCWC 1 cut(s) 306
BbvI GCAGC 1 cut(s) 368
BccI CCATC 4 cut(s) 31, 110, 218, 443
BfaI CTAG 2 cut(s) 27, 332
BfmI CTRYAG 1 cut(s) 354
BglI GCCNNNNNGGC 1 cut(s) 161
BisI GCNGC 1 cut(s) 357
BlsI GCNGC 1 cut(s) 358
BmeT110I CYCGRG 1 cut(s) 121
BmgT120I GGNCC 1 cut(s) 20
BmsI GCATC 1 cut(s) 124
BsaJI CCNNGG 1 cut(s) 138
Bse3DI GCAATG 2 cut(s) 346, 372
BseDI CCNNGG 1 cut(s) 138
BseGI GGATG 2 cut(s) 229, 486
BseMI GCAATG 2 cut(s) 346, 372
BseRI GAGGAG 1 cut(s) 314
BseSI GKGCMC 1 cut(s) 128
BseXI GCAGC 1 cut(s) 368
BshFI GGCC 1 cut(s) 22
BsiHKAI GWGCWC 1 cut(s) 306
BsiHKCI CYCGRG 1 cut(s) 121
BslFI GGGAC 1 cut(s) 302
BsmFI GGGAC 1 cut(s) 302
BsmI GAATGC 2 cut(s) 95, 314
BsnI GGCC 1 cut(s) 22
BsoBI CYCGRG 1 cut(s) 121
Bsp1286I GDGCHC 3 cut(s) 128, 166, 306
Bsp143I GATC 4 cut(s) 334, 382, 411, 442
BspACI CCGC 1 cut(s) 270
BspANI GGCC 1 cut(s) 22
BspHI TCATGA 1 cut(s) 306
BspMAI CTGCAG 1 cut(s) 358
BspPI GGATC 2 cut(s) 390, 406
BspQI GCTCTTC 1 cut(s) 180
BsrDI GCAATG 2 cut(s) 346, 372
BssECI CCNNGG 1 cut(s) 138
BssMI GATC 4 cut(s) 334, 382, 411, 442
BssT1I CCWWGG 1 cut(s) 138
Bst6I CTCTTC 2 cut(s) 180, 482
BstC8I GCNNGC 1 cut(s) 7
BstF5I GGATG 2 cut(s) 229, 486
BstKTI GATC 4 cut(s) 337, 385, 414, 445
BstMBI GATC 4 cut(s) 334, 382, 411, 442
BstMWI GCNNNNNNNGC 1 cut(s) 161
BstSFI CTRYAG 1 cut(s) 354
BstSLI GKGCMC 1 cut(s) 128
BstV1I GCAGC 1 cut(s) 368
BstX2I RGATCY 2 cut(s) 382, 411
BstYI RGATCY 2 cut(s) 382, 411
BsuRI GGCC 1 cut(s) 22
BtsCI GGATG 2 cut(s) 229, 486
BtsI GCAGTG 1 cut(s) 235
BtsIMutI CAGTG 2 cut(s) 235, 399
Cac8I GCNNGC 1 cut(s) 7
CciI TCATGA 1 cut(s) 306
Cfr13I GGNCC 1 cut(s) 20
CviAII CATG 3 cut(s) 157, 262, 307
CviJI RGCY 7 cut(s) 9, 22, 120, 145, 164, 304, 322
CviKI_1 RGCY 7 cut(s) 9, 22, 120, 145, 164, 304, 322
DpnI GATC 4 cut(s) 336, 384, 413, 444
DpnII GATC 4 cut(s) 334, 382, 411, 442
Eam1104I CTCTTC 2 cut(s) 180, 482
EarI CTCTTC 2 cut(s) 180, 482
Ecl136II GAGCTC 1 cut(s) 304
Eco130I CCWWGG 1 cut(s) 138
Eco24I GRGCYC 2 cut(s) 166, 306
Eco32I GATATC 1 cut(s) 351
Eco53kI GAGCTC 1 cut(s) 304
Eco88I CYCGRG 1 cut(s) 121
EcoICRI GAGCTC 1 cut(s) 304
EcoRV GATATC 1 cut(s) 351
EcoT14I CCWWGG 1 cut(s) 138
EcoT38I GRGCYC 2 cut(s) 166, 306
ErhI CCWWGG 1 cut(s) 138
FaeI CATG 3 cut(s) 160, 265, 310
FaiI YATR 4 cut(s) 158, 263, 308, 499
FaqI GGGAC 1 cut(s) 302
FatI CATG 3 cut(s) 156, 261, 306
Fnu4HI GCNGC 1 cut(s) 357
FokI GGATG 2 cut(s) 236, 493
FriOI GRGCYC 2 cut(s) 166, 306
Fsp4HI GCNGC 1 cut(s) 357
FspBI CTAG 2 cut(s) 27, 332
GluI GCNGC 1 cut(s) 357
HaeIII GGCC 1 cut(s) 22
Hin1II CATG 3 cut(s) 160, 265, 310
HincII GTYRAC 2 cut(s) 63, 217
HindII GTYRAC 2 cut(s) 63, 217
HpaI GTTAAC 1 cut(s) 63
HphI GGTGA 1 cut(s) 401
Hpy166II GTNNAC 2 cut(s) 63, 217
Hpy188III TCNNGA 3 cut(s) 307, 446, 478
Hpy8I GTNNAC 2 cut(s) 63, 217
HpyAV CCTTC 1 cut(s) 25
HpyCH4V TGCA 3 cut(s) 209, 356, 377
HpyF10VI GCNNNNNNNGC 1 cut(s) 161
Hsp92II CATG 3 cut(s) 160, 265, 310
KspAI GTTAAC 1 cut(s) 63
Kzo9I GATC 4 cut(s) 334, 382, 411, 442
LguI GCTCTTC 1 cut(s) 180
LmnI GCTCC 2 cut(s) 142, 301
LpnPI CCDG 4 cut(s) 55, 332, 371, 463
Lsp1109I GCAGC 1 cut(s) 368
LweI GCATC 1 cut(s) 124
MaeI CTAG 2 cut(s) 27, 332
MalI GATC 4 cut(s) 336, 384, 413, 444
MboI GATC 4 cut(s) 334, 382, 411, 442
MboII GAAGA 2 cut(s) 197, 499
MflI RGATCY 2 cut(s) 382, 411
MhlI GDGCHC 3 cut(s) 128, 166, 306
MluCI AATT 2 cut(s) 192, 276
MnlI CCTC 7 cut(s) 11, 38, 69, 85, 153, 292, 421
MroXI GAANNNNTTC 1 cut(s) 314
MseI TTAA 4 cut(s) 62, 87, 366, 459
Mva1269I GAATGC 2 cut(s) 95, 314
MwoI GCNNNNNNNGC 1 cut(s) 161
NdeII GATC 4 cut(s) 334, 382, 411, 442
NlaIII CATG 3 cut(s) 160, 265, 310
PagI TCATGA 1 cut(s) 306
PciSI GCTCTTC 1 cut(s) 180
PcsI WCGNNNNNNNCGW 1 cut(s) 105
PctI GAATGC 2 cut(s) 95, 314
PdmI GAANNNNTTC 1 cut(s) 314
PkrI GCNGC 1 cut(s) 358
Psp124BI GAGCTC 1 cut(s) 306
PspPI GGNCC 1 cut(s) 20
PstI CTGCAG 1 cut(s) 358
PsuI RGATCY 2 cut(s) 382, 411
SacI GAGCTC 1 cut(s) 306
SapI GCTCTTC 1 cut(s) 180
SaqAI TTAA 4 cut(s) 62, 87, 366, 459
SatI GCNGC 1 cut(s) 357
Sau3AI GATC 4 cut(s) 334, 382, 411, 442
Sau96I GGNCC 1 cut(s) 20
SduI GDGCHC 3 cut(s) 128, 166, 306
SetI ASST 4 cut(s) 147, 306, 324, 333
SfaNI GCATC 1 cut(s) 124
SfcI CTRYAG 1 cut(s) 354
Sse9I AATT 2 cut(s) 192, 276
SsiI CCGC 1 cut(s) 270
SspMI CTAG 2 cut(s) 27, 332
SstI GAGCTC 1 cut(s) 306
StyI CCWWGG 1 cut(s) 138
TaqI TCGA 3 cut(s) 99, 108, 240
TaqII GACCGA 2 cut(s) 197, 453
TasI AATT 2 cut(s) 192, 276
Tru1I TTAA 4 cut(s) 62, 87, 366, 459
Tru9I TTAA 4 cut(s) 62, 87, 366, 459
TscAI CASTG 2 cut(s) 235, 406
TseI GCWGC 1 cut(s) 356
TspDTI ATGAA 3 cut(s) 185, 323, 500
TspRI CASTG 2 cut(s) 235, 406
XapI RAATTY 1 cut(s) 192
XmnI GAANNNNTTC 1 cut(s) 314
XspI CTAG 2 cut(s) 27, 332
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.