FvH4_1g15980

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0013400
Physical Location & Seq
Reverse (-)
9085078 .. 9087087
2010 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g15980.t1

Sequence Viewer

Length: 612 bp
ATGAATCTTAAAACGAACATTGAGCTTCTGATCGAAGAGAGATCAAGGTCATCAACAACACACAACTTTCTTCCACTTTCCTATTCAACGCGTTTCCAAATGGCGACCCTATACAGAGGGCCTTCTAGGAAAGAGAAACCTCGGGCAGGGAGGCATCATGGGTTGAATCAGCAAAAGAGGCAAGAAATTAAGGAAGCTTTCGAGTTGTTCGACACTGATGGCTCAGGCACTATTGATGCCAAAGAGTTGAATGTTGCCATGAGGGCTCTTGGATTCGAGATGACAGAAGAGCAAATTAACCAAATGATTGCAGATGTTGATAAGGATGGAAGTGGAGCCATTGACTTTGATGAGTTTGCTCACATGATGACAGCCAAAATTGGGGAACGGGACACAAAGGAGGAACTCATGAAAGCATTCCATATCCTTGACCAAGATAACAATGGCAAGATATCTACTGCAGACATTAAGAAGATTGCGAAGGAGCTGGGTGAGACTTTCACCGATAGAGAGATTCAAGAGATGGTTGAGGAAGCAGACCGAGACCGTGATGGAGAAGTTAGTGCTGATGAGTTCATCAGGATGATGAAGAGAACAACATATGGCTATTAG

Protein Analysis

204

Amino Acids

23.34

Weight (kDa)

5.1

Isoelectric Point (pI)

33.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AIF-1 PF21008 58 - 126 3.8e-06 Allograft inflammatory factor 1
EF-hand_7 PF13499 61 - 124 3.4e-18 EF-hand domain pair
EF-hand_1 PF00036 62 - 90 2.4e-08 EF hand domain
EF-hand_6 PF13405 62 - 91 2.5e-08 EF-hand domain
EF-hand_5 PF13202 63 - 84 4.5e-06 EF hand
EF-hand_9 PF14658 65 - 126 1.2e-06 EF-hand domain
EF-hand_8 PF13833 76 - 124 6.9e-07 EF-hand domain pair
EF-hand_1 PF00036 99 - 125 1.1e-07 EF hand domain
EF-hand_7 PF13499 133 - 197 1.6e-16 EF-hand domain pair
EF-hand_6 PF13405 135 - 164 4.2e-06 EF-hand domain
EF-hand_8 PF13833 148 - 198 1.2e-11 EF-hand domain pair
EF-hand_1 PF00036 171 - 198 1.6e-07 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013400)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G50360 AT4G37010 AT4G37010
fragaria_vesca FvH4_1g15980
malus_domestica MD02G1171500.v1.1 MD15G1283300.v1.1
prunus_persica Prupe.7G133900_v2.0.a1 Prupe.7G133900_v2.0.a1
pyrus_communis pycom02g13820 pycom15g24650
rosa_chinensis RchiOBHm_Chr2g0105581
rosa_laevigata RLG00000017438
rosa_rugosa Rorug02G0128400
rosa_samantha Rh2AG180000 Rh2BG189100 Rh2CG184300 Rh2DG185800
rosa_wichuraiana Rw2G014080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 91
AfiI CCNNNNNNNGG 2 cut(s) 146, 381
AflIII ACRYGT 1 cut(s) 89
AgsI TTSAA 4 cut(s) 87, 166, 250, 518
AluBI AGCT 3 cut(s) 25, 197, 487
AluI AGCT 3 cut(s) 25, 197, 487
Alw26I GTCTC 2 cut(s) 488, 537
Ama87I CYCGRG 1 cut(s) 141
AoxI GGCC 1 cut(s) 119
Asp700I GAANNNNTTC 1 cut(s) 416
AspS9I GGNCC 1 cut(s) 119
AsuHPI GGTGA 2 cut(s) 493, 503
AvaI CYCGRG 1 cut(s) 141
BanII GRGCYC 1 cut(s) 268
BccI CCATC 4 cut(s) 212, 320, 517, 545
BcoDI GTCTC 2 cut(s) 488, 537
BfaI CTAG 1 cut(s) 126
BfmI CTRYAG 1 cut(s) 459
BglI GCCNNNNNGGC 1 cut(s) 263
BmeT110I CYCGRG 1 cut(s) 141
BmgT120I GGNCC 1 cut(s) 119
BmiI GGNNCC 1 cut(s) 337
BmsI GCATC 2 cut(s) 163, 226
Bpu10I CCTNAGC 1 cut(s) 223
BsaI GGTCTC 1 cut(s) 537
BsaJI CCNNGG 1 cut(s) 140
Bsc4I CCNNNNNNNGG 2 cut(s) 146, 381
BseDI CCNNGG 1 cut(s) 140
BseGI GGATG 2 cut(s) 331, 588
BseLI CCNNNNNNNGG 2 cut(s) 146, 381
BseMII CTCAG 1 cut(s) 237
BseYI CCCAGC 1 cut(s) 487
Bsh1236I CGCG 1 cut(s) 91
BshFI GGCC 1 cut(s) 121
BsiHKCI CYCGRG 1 cut(s) 141
BslFI GGGAC 1 cut(s) 404
BslI CCNNNNNNNGG 2 cut(s) 146, 381
BsmAI GTCTC 2 cut(s) 488, 537
BsmFI GGGAC 1 cut(s) 404
BsmI GAATGC 1 cut(s) 416
BsnI GGCC 1 cut(s) 121
Bso31I GGTCTC 1 cut(s) 537
BsoBI CYCGRG 1 cut(s) 141
Bsp1286I GDGCHC 1 cut(s) 268
Bsp143I GATC 2 cut(s) 30, 41
BspANI GGCC 1 cut(s) 121
BspCNI CTCAG 1 cut(s) 236
BspFNI CGCG 1 cut(s) 91
BspHI TCATGA 1 cut(s) 408
BspLI GGNNCC 1 cut(s) 337
BspMAI CTGCAG 1 cut(s) 463
BspQI GCTCTTC 1 cut(s) 282
BspTNI GGTCTC 1 cut(s) 537
BssECI CCNNGG 1 cut(s) 140
BssMI GATC 2 cut(s) 30, 41
Bst4CI ACNGT 1 cut(s) 548
Bst6I CTCTTC 3 cut(s) 30, 282, 584
BstDEI CTNAG 1 cut(s) 223
BstENI CCTNNNNNAGG 1 cut(s) 144
BstF5I GGATG 2 cut(s) 331, 588
BstFNI CGCG 1 cut(s) 91
BstKTI GATC 2 cut(s) 33, 44
BstMAI GTCTC 2 cut(s) 488, 537
BstMBI GATC 2 cut(s) 30, 41
BstMWI GCNNNNNNNGC 2 cut(s) 178, 263
BstSFI CTRYAG 1 cut(s) 459
BstUI CGCG 1 cut(s) 91
BsuRI GGCC 1 cut(s) 121
BtsCI GGATG 2 cut(s) 331, 588
BtsIMutI CAGTG 1 cut(s) 213
CciI TCATGA 1 cut(s) 408
Cfr13I GGNCC 1 cut(s) 119
CviAII CATG 4 cut(s) 158, 259, 364, 409
CviJI RGCY 9 cut(s) 25, 121, 197, 222, 266, 338, 374, 487, 606
CviKI_1 RGCY 9 cut(s) 25, 121, 197, 222, 266, 338, 374, 487, 606
DdeI CTNAG 1 cut(s) 223
DpnI GATC 2 cut(s) 32, 43
DpnII GATC 2 cut(s) 30, 41
Eam1104I CTCTTC 3 cut(s) 30, 282, 584
EarI CTCTTC 3 cut(s) 30, 282, 584
Eco24I GRGCYC 1 cut(s) 268
Eco31I GGTCTC 1 cut(s) 537
Eco32I GATATC 1 cut(s) 453
Eco88I CYCGRG 1 cut(s) 141
EcoNI CCTNNNNNAGG 1 cut(s) 144
EcoO109I RGGNCCY 1 cut(s) 119
EcoRV GATATC 1 cut(s) 453
EcoT38I GRGCYC 1 cut(s) 268
FaeI CATG 4 cut(s) 161, 262, 367, 412
FaiI YATR 8 cut(s) 112, 159, 260, 365, 410, 423, 601, 603
FaqI GGGAC 1 cut(s) 404
FatI CATG 4 cut(s) 157, 258, 363, 408
FauNDI CATATG 1 cut(s) 601
FokI GGATG 2 cut(s) 338, 595
FriOI GRGCYC 1 cut(s) 268
FspBI CTAG 1 cut(s) 126
GsaI CCCAGC 1 cut(s) 491
HaeIII GGCC 1 cut(s) 121
Hin1II CATG 4 cut(s) 161, 262, 367, 412
HindIII AAGCTT 1 cut(s) 195
HinfI GANTC 4 cut(s) 4, 166, 273, 514
HphI GGTGA 2 cut(s) 493, 503
Hpy188I TCNGA 1 cut(s) 30
Hpy188III TCNNGA 4 cut(s) 277, 409, 518, 580
HpyAV CCTTC 2 cut(s) 132, 475
HpyCH4III ACNGT 1 cut(s) 548
HpyCH4V TGCA 2 cut(s) 311, 461
HpyF10VI GCNNNNNNNGC 2 cut(s) 178, 263
HpyF3I CTNAG 1 cut(s) 223
Hsp92II CATG 4 cut(s) 161, 262, 367, 412
Kzo9I GATC 2 cut(s) 30, 41
LguI GCTCTTC 1 cut(s) 282
LmnI GCTCC 2 cut(s) 335, 484
LpnPI CCDG 4 cut(s) 132, 210, 473, 565
LweI GCATC 2 cut(s) 163, 226
MaeI CTAG 1 cut(s) 126
MalI GATC 2 cut(s) 32, 43
MboI GATC 2 cut(s) 30, 41
MboII GAAGA 5 cut(s) 47, 62, 299, 484, 601
MhlI GDGCHC 1 cut(s) 268
MluCI AATT 3 cut(s) 186, 294, 378
MluI ACGCGT 1 cut(s) 89
MnlI CCTC 7 cut(s) 110, 144, 150, 171, 255, 394, 523
MroXI GAANNNNTTC 1 cut(s) 416
MseI TTAA 4 cut(s) 9, 189, 297, 468
MslI CAYNNNNRTG 1 cut(s) 581
Mva1269I GAATGC 1 cut(s) 416
MvnI CGCG 1 cut(s) 91
MwoI GCNNNNNNNGC 2 cut(s) 178, 263
NdeI CATATG 1 cut(s) 601
NdeII GATC 2 cut(s) 30, 41
NlaIII CATG 4 cut(s) 161, 262, 367, 412
NlaIV GGNNCC 1 cut(s) 337
PagI TCATGA 1 cut(s) 408
PciSI GCTCTTC 1 cut(s) 282
PcsI WCGNNNNNNNCGW 1 cut(s) 207
PctI GAATGC 1 cut(s) 416
PdmI GAANNNNTTC 1 cut(s) 416
PfeI GAWTC 4 cut(s) 4, 166, 273, 514
PspFI CCCAGC 1 cut(s) 487
PspN4I GGNNCC 1 cut(s) 337
PspPI GGNCC 1 cut(s) 119
PstI CTGCAG 1 cut(s) 463
RseI CAYNNNNRTG 1 cut(s) 581
SapI GCTCTTC 1 cut(s) 282
SaqAI TTAA 4 cut(s) 9, 189, 297, 468
Sau3AI GATC 2 cut(s) 30, 41
Sau96I GGNCC 1 cut(s) 119
SduI GDGCHC 1 cut(s) 268
SetI ASST 5 cut(s) 27, 50, 142, 199, 489
SfaNI GCATC 2 cut(s) 163, 226
SfcI CTRYAG 1 cut(s) 459
SmiMI CAYNNNNRTG 1 cut(s) 581
Sse9I AATT 3 cut(s) 186, 294, 378
SspMI CTAG 1 cut(s) 126
TaaI ACNGT 1 cut(s) 548
TaqI TCGA 4 cut(s) 33, 201, 210, 276
TaqII GACCGA 1 cut(s) 555
TasI AATT 3 cut(s) 186, 294, 378
TfiI GAWTC 4 cut(s) 4, 166, 273, 514
Tru1I TTAA 4 cut(s) 9, 189, 297, 468
Tru9I TTAA 4 cut(s) 9, 189, 297, 468
TscAI CASTG 1 cut(s) 220
TspDTI ATGAA 4 cut(s) 17, 425, 565, 602
TspRI CASTG 1 cut(s) 220
XagI CCTNNNNNAGG 1 cut(s) 144
XcmI CCANNNNNNNNNTGG 1 cut(s) 440
XmnI GAANNNNTTC 1 cut(s) 416
XspI CTAG 1 cut(s) 126
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.