RLG00000017438

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0013400
Physical Location & Seq
Reverse (-)
16733280 .. 16734956
1677 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017438

Sequence Viewer

Length: 510 bp
ATGGAAAGACTCTATAGAGGGCCTTCTAGGAAAGAGAAACCTAGAGCGAGACATCATGGGTTAAATCAGCAAAAAAGGCAAGAGATTAAGGAAGCTTTCGAGTTGTTCGACACTGATGGCTCAGGCACTATTGATGCCAAAGAGTTGAATGTTGCCATGAGGGCTCTTGGATTCGAGATGACAGAAGAGCAAATTAACCAAATGATTGCAGATGTTGACAAGGACGGAAGTGGTGCCATTGATTTTGATGAGTTTGCTCACATGATGACAGCCAAAATCGGGGAACGGGACACAAAGGAGGAACTCATGAAAGCATTCCATATCCTTGACCAAGATAACAATGGCAAGATATCTACTTTAGACATTAAGAAGATTGCAAAAGAGCTGGGTGAGAATTTCACTGATAGAGATATTCAAGAGATGGTTGAGGAAGCAGACCGAGACCGCGATGGAGAAGTCAGTGCTGAGGAGTTCATCAGGATGATGAAGAGAACAACATATGGCTATTAG

Protein Analysis

170

Amino Acids

19.54

Weight (kDa)

4.92

Isoelectric Point (pI)

19.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AIF-1 PF21008 23 - 92 2.7e-06 Allograft inflammatory factor 1
EF-hand_7 PF13499 27 - 90 2.3e-18 EF-hand domain pair
EF-hand_1 PF00036 28 - 56 1.8e-08 EF hand domain
EF-hand_6 PF13405 28 - 57 1.8e-08 EF-hand domain
EF-hand_5 PF13202 29 - 50 3.5e-06 EF hand
EF-hand_9 PF14658 31 - 92 8.1e-07 EF-hand domain
EF-hand_8 PF13833 41 - 90 1.8e-07 EF-hand domain pair
EF-hand_1 PF00036 65 - 91 8.3e-08 EF hand domain
EF-hand_7 PF13499 99 - 163 1.2e-15 EF-hand domain pair
EF-hand_6 PF13405 101 - 130 6.2e-06 EF-hand domain
EF-hand_8 PF13833 123 - 164 1e-08 EF-hand domain pair
EF-hand_1 PF00036 137 - 164 1.6e-07 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0013400)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G50360 AT4G37010 AT4G37010
fragaria_vesca FvH4_1g15980
malus_domestica MD02G1171500.v1.1 MD15G1283300.v1.1
prunus_persica Prupe.7G133900_v2.0.a1 Prupe.7G133900_v2.0.a1
pyrus_communis pycom02g13820 pycom15g24650
rosa_chinensis RchiOBHm_Chr2g0105581
rosa_laevigata RLG00000017438
rosa_rugosa Rorug02G0128400
rosa_samantha Rh2AG180000 Rh2BG189100 Rh2CG184300 Rh2DG185800
rosa_wichuraiana Rw2G014080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 233
AccII CGCG 1 cut(s) 447
AciI CCGC 1 cut(s) 445
AcsI RAATTY 1 cut(s) 394
AfiI CCNNNNNNNGG 1 cut(s) 279
AgsI TTSAA 2 cut(s) 148, 416
AluBI AGCT 2 cut(s) 95, 385
AluI AGCT 2 cut(s) 95, 385
Alw26I GTCTC 2 cut(s) 43, 435
AoxI GGCC 1 cut(s) 20
ApoI RAATTY 1 cut(s) 394
Asp700I GAANNNNTTC 1 cut(s) 314
AspS9I GGNCC 1 cut(s) 20
AsuHPI GGTGA 1 cut(s) 401
BanI GGYRCC 1 cut(s) 233
BanII GRGCYC 1 cut(s) 166
BbvCI CCTCAGC 1 cut(s) 465
BccI CCATC 3 cut(s) 110, 415, 443
BcoDI GTCTC 2 cut(s) 43, 435
BfaI CTAG 2 cut(s) 27, 42
BfmI CTRYAG 1 cut(s) 13
BglI GCCNNNNNGGC 1 cut(s) 161
BmgT120I GGNCC 1 cut(s) 20
BmiI GGNNCC 1 cut(s) 235
BmsI GCATC 1 cut(s) 124
Bpu10I CCTNAGC 2 cut(s) 121, 465
BsaI GGTCTC 1 cut(s) 435
Bsc4I CCNNNNNNNGG 1 cut(s) 279
BseGI GGATG 1 cut(s) 486
BseLI CCNNNNNNNGG 1 cut(s) 279
BseMII CTCAG 2 cut(s) 135, 456
BseRI GAGGAG 1 cut(s) 482
BseYI CCCAGC 1 cut(s) 385
Bsh1236I CGCG 1 cut(s) 447
BshFI GGCC 1 cut(s) 22
BshNI GGYRCC 1 cut(s) 233
BslFI GGGAC 1 cut(s) 302
BslI CCNNNNNNNGG 1 cut(s) 279
BsmAI GTCTC 2 cut(s) 43, 435
BsmFI GGGAC 1 cut(s) 302
BsmI GAATGC 1 cut(s) 314
BsnI GGCC 1 cut(s) 22
Bso31I GGTCTC 1 cut(s) 435
Bsp1286I GDGCHC 1 cut(s) 166
BspACI CCGC 1 cut(s) 445
BspANI GGCC 1 cut(s) 22
BspCNI CTCAG 2 cut(s) 134, 457
BspFNI CGCG 1 cut(s) 447
BspHI TCATGA 1 cut(s) 306
BspLI GGNNCC 1 cut(s) 235
BspQI GCTCTTC 1 cut(s) 180
BspT107I GGYRCC 1 cut(s) 233
BspTNI GGTCTC 1 cut(s) 435
Bst6I CTCTTC 2 cut(s) 180, 482
BstDEI CTNAG 2 cut(s) 121, 465
BstF5I GGATG 1 cut(s) 486
BstFNI CGCG 1 cut(s) 447
BstMAI GTCTC 2 cut(s) 43, 435
BstMWI GCNNNNNNNGC 2 cut(s) 76, 161
BstSFI CTRYAG 1 cut(s) 13
BstUI CGCG 1 cut(s) 447
BsuRI GGCC 1 cut(s) 22
BtgZI GCGATG 1 cut(s) 462
BtsCI GGATG 1 cut(s) 486
BtsIMutI CAGTG 3 cut(s) 111, 399, 466
CciI TCATGA 1 cut(s) 306
Cfr13I GGNCC 1 cut(s) 20
CviAII CATG 4 cut(s) 56, 157, 262, 307
CviJI RGCY 7 cut(s) 22, 95, 120, 164, 272, 385, 504
CviKI_1 RGCY 7 cut(s) 22, 95, 120, 164, 272, 385, 504
DdeI CTNAG 2 cut(s) 121, 465
Eam1104I CTCTTC 2 cut(s) 180, 482
EarI CTCTTC 2 cut(s) 180, 482
Eco24I GRGCYC 1 cut(s) 166
Eco31I GGTCTC 1 cut(s) 435
Eco32I GATATC 1 cut(s) 351
EcoO109I RGGNCCY 1 cut(s) 20
EcoRV GATATC 1 cut(s) 351
EcoT38I GRGCYC 1 cut(s) 166
FaeI CATG 4 cut(s) 59, 160, 265, 310
FaiI YATR 8 cut(s) 15, 57, 158, 263, 308, 321, 499, 501
FaqI GGGAC 1 cut(s) 302
FatI CATG 4 cut(s) 55, 156, 261, 306
FauNDI CATATG 1 cut(s) 499
FokI GGATG 1 cut(s) 493
FriOI GRGCYC 1 cut(s) 166
FspBI CTAG 2 cut(s) 27, 42
GsaI CCCAGC 1 cut(s) 389
HaeIII GGCC 1 cut(s) 22
Hin1II CATG 4 cut(s) 59, 160, 265, 310
HincII GTYRAC 1 cut(s) 217
HindII GTYRAC 1 cut(s) 217
HindIII AAGCTT 1 cut(s) 93
HinfI GANTC 2 cut(s) 9, 171
HphI GGTGA 1 cut(s) 401
Hpy166II GTNNAC 1 cut(s) 217
Hpy188III TCNNGA 4 cut(s) 175, 307, 416, 478
Hpy8I GTNNAC 1 cut(s) 217
HpyAV CCTTC 1 cut(s) 33
HpyCH4V TGCA 2 cut(s) 209, 377
HpyF10VI GCNNNNNNNGC 2 cut(s) 76, 161
HpyF3I CTNAG 2 cut(s) 121, 465
Hsp92II CATG 4 cut(s) 59, 160, 265, 310
LguI GCTCTTC 1 cut(s) 180
LpnPI CCDG 3 cut(s) 108, 371, 463
LweI GCATC 1 cut(s) 124
MaeI CTAG 2 cut(s) 27, 42
MboII GAAGA 3 cut(s) 197, 382, 499
MhlI GDGCHC 1 cut(s) 166
MluCI AATT 2 cut(s) 192, 394
MlyI GAGTC 1 cut(s) 3
MnlI CCTC 5 cut(s) 11, 153, 292, 421, 460
MroXI GAANNNNTTC 1 cut(s) 314
MseI TTAA 4 cut(s) 62, 87, 195, 366
MslI CAYNNNNRTG 1 cut(s) 479
Mva1269I GAATGC 1 cut(s) 314
MvnI CGCG 1 cut(s) 447
MwoI GCNNNNNNNGC 2 cut(s) 76, 161
NdeI CATATG 1 cut(s) 499
NlaIII CATG 4 cut(s) 59, 160, 265, 310
NlaIV GGNNCC 1 cut(s) 235
PagI TCATGA 1 cut(s) 306
PciSI GCTCTTC 1 cut(s) 180
PcsI WCGNNNNNNNCGW 1 cut(s) 105
PctI GAATGC 1 cut(s) 314
PdmI GAANNNNTTC 1 cut(s) 314
PfeI GAWTC 1 cut(s) 171
PleI GAGTC 1 cut(s) 3
PpsI GAGTC 1 cut(s) 3
PspFI CCCAGC 1 cut(s) 385
PspN4I GGNNCC 1 cut(s) 235
PspPI GGNCC 1 cut(s) 20
RseI CAYNNNNRTG 1 cut(s) 479
SapI GCTCTTC 1 cut(s) 180
SaqAI TTAA 4 cut(s) 62, 87, 195, 366
Sau96I GGNCC 1 cut(s) 20
SchI GAGTC 1 cut(s) 3
SduI GDGCHC 1 cut(s) 166
SetI ASST 3 cut(s) 43, 97, 387
SfaNI GCATC 1 cut(s) 124
SfcI CTRYAG 1 cut(s) 13
SmiMI CAYNNNNRTG 1 cut(s) 479
Sse9I AATT 2 cut(s) 192, 394
SsiI CCGC 1 cut(s) 445
SspMI CTAG 2 cut(s) 27, 42
TaqI TCGA 3 cut(s) 99, 108, 174
TaqII GACCGA 1 cut(s) 453
TasI AATT 2 cut(s) 192, 394
TfiI GAWTC 1 cut(s) 171
Tru1I TTAA 4 cut(s) 62, 87, 195, 366
Tru9I TTAA 4 cut(s) 62, 87, 195, 366
TscAI CASTG 3 cut(s) 118, 406, 466
TspDTI ATGAA 3 cut(s) 323, 463, 500
TspGWI ACGGA 1 cut(s) 240
TspRI CASTG 3 cut(s) 118, 406, 466
XapI RAATTY 1 cut(s) 394
XcmI CCANNNNNNNNNTGG 1 cut(s) 338
XmnI GAANNNNTTC 1 cut(s) 314
XspI CTAG 2 cut(s) 27, 42
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.