Rh2DG185800

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0013400
Physical Location & Seq
Reverse (-)
16588735 .. 16590261
1527 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG185800.1

Sequence Viewer

Length: 465 bp
ATGGCAAGCCTCTATGGAGGGCCTTCTAGGAAAGAGAAACCTAGAGCGAGACATCATGGGTTAAATCAGCAAAAGAGGCAAGAGATTAAGGAAGCCTTCGAGTTGTTCGACACTGATGGCTCAGGCACTATTGATGCCAAAGAGTTGAATGTTGCCATGAGGGCTCTTGGATTCGAGATGACAGAAGAGCAAATTAACCAAATGATTGCAGATGTTGACAAGGACGGAAGTGGTGCCATTGATTTTGATGAGTTTGCTCACATGATGACAGCCAAAATCGGGGAACGGGACACAAAGGAGGAACTCATGAAAGCATTCCATATCCTTGACCAAGATAACAATGGCAAGATATCTACTTTAGACATTAAGAAGATTGCAAAAGAGCTGGGTGAGAATTTCACTGATAGAGATATTCAAGAGATGGTTGAGGAAGCAGACCGAGACCGTGAGTACTTCAACTTCTGA

Protein Analysis

154

Amino Acids

17.65

Weight (kDa)

4.78

Isoelectric Point (pI)

19.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AIF-1 PF21008 23 - 92 1.8e-06 Allograft inflammatory factor 1
EF-hand_7 PF13499 27 - 90 1.9e-18 EF-hand domain pair
EF-hand_1 PF00036 28 - 56 1.5e-08 EF hand domain
EF-hand_6 PF13405 28 - 57 1.6e-08 EF-hand domain
EF-hand_5 PF13202 29 - 50 3e-06 EF hand
EF-hand_9 PF14658 31 - 92 6.6e-07 EF-hand domain
EF-hand_8 PF13833 41 - 90 1.6e-07 EF-hand domain pair
EF-hand_1 PF00036 65 - 91 7.2e-08 EF hand domain
EF-hand_7 PF13499 99 - 149 3.6e-09 EF-hand domain pair
EF-hand_6 PF13405 101 - 130 5.3e-06 EF-hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013400)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G50360 AT4G37010 AT4G37010
fragaria_vesca FvH4_1g15980
malus_domestica MD02G1171500.v1.1 MD15G1283300.v1.1
prunus_persica Prupe.7G133900_v2.0.a1 Prupe.7G133900_v2.0.a1
pyrus_communis pycom02g13820 pycom15g24650
rosa_chinensis RchiOBHm_Chr2g0105581
rosa_laevigata RLG00000017438
rosa_rugosa Rorug02G0128400
rosa_samantha Rh2AG180000 Rh2BG189100 Rh2CG184300 Rh2DG185800
rosa_wichuraiana Rw2G014080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 233
AcsI RAATTY 1 cut(s) 394
AfaI GTAC 1 cut(s) 452
AfiI CCNNNNNNNGG 1 cut(s) 279
AgsI TTSAA 3 cut(s) 148, 416, 457
AluBI AGCT 1 cut(s) 385
AluI AGCT 1 cut(s) 385
Alw26I GTCTC 2 cut(s) 43, 435
AoxI GGCC 1 cut(s) 20
ApoI RAATTY 1 cut(s) 394
Asp700I GAANNNNTTC 1 cut(s) 314
AspS9I GGNCC 1 cut(s) 20
AsuHPI GGTGA 1 cut(s) 401
BanI GGYRCC 1 cut(s) 233
BanII GRGCYC 1 cut(s) 166
BarI GAAGNNNNNNTAC 1 cut(s) 443
BccI CCATC 2 cut(s) 110, 415
BcoDI GTCTC 2 cut(s) 43, 435
BfaI CTAG 2 cut(s) 27, 42
BglI GCCNNNNNGGC 1 cut(s) 161
BmcAI AGTACT 1 cut(s) 452
BmgT120I GGNCC 1 cut(s) 20
BmiI GGNNCC 1 cut(s) 235
BmsI GCATC 1 cut(s) 124
Bpu10I CCTNAGC 1 cut(s) 121
BsaI GGTCTC 1 cut(s) 435
Bsc4I CCNNNNNNNGG 1 cut(s) 279
BseLI CCNNNNNNNGG 1 cut(s) 279
BseMII CTCAG 1 cut(s) 135
BseYI CCCAGC 1 cut(s) 385
BshFI GGCC 1 cut(s) 22
BshNI GGYRCC 1 cut(s) 233
BslFI GGGAC 1 cut(s) 302
BslI CCNNNNNNNGG 1 cut(s) 279
BsmAI GTCTC 2 cut(s) 43, 435
BsmFI GGGAC 1 cut(s) 302
BsmI GAATGC 1 cut(s) 314
BsnI GGCC 1 cut(s) 22
Bso31I GGTCTC 1 cut(s) 435
Bsp1286I GDGCHC 1 cut(s) 166
BspANI GGCC 1 cut(s) 22
BspCNI CTCAG 1 cut(s) 134
BspHI TCATGA 1 cut(s) 306
BspLI GGNNCC 1 cut(s) 235
BspQI GCTCTTC 1 cut(s) 180
BspT107I GGYRCC 1 cut(s) 233
BspTNI GGTCTC 1 cut(s) 435
Bst4CI ACNGT 1 cut(s) 446
Bst6I CTCTTC 1 cut(s) 180
BstC8I GCNNGC 1 cut(s) 7
BstDEI CTNAG 1 cut(s) 121
BstMAI GTCTC 2 cut(s) 43, 435
BstMWI GCNNNNNNNGC 2 cut(s) 76, 161
BsuRI GGCC 1 cut(s) 22
BtsIMutI CAGTG 2 cut(s) 111, 399
Cac8I GCNNGC 1 cut(s) 7
CciI TCATGA 1 cut(s) 306
Cfr13I GGNCC 1 cut(s) 20
Csp6I GTAC 1 cut(s) 451
CviAII CATG 4 cut(s) 56, 157, 262, 307
CviJI RGCY 7 cut(s) 9, 22, 95, 120, 164, 272, 385
CviKI_1 RGCY 7 cut(s) 9, 22, 95, 120, 164, 272, 385
CviQI GTAC 1 cut(s) 451
DdeI CTNAG 1 cut(s) 121
Eam1104I CTCTTC 1 cut(s) 180
EarI CTCTTC 1 cut(s) 180
Eco24I GRGCYC 1 cut(s) 166
Eco31I GGTCTC 1 cut(s) 435
Eco32I GATATC 1 cut(s) 351
EcoO109I RGGNCCY 1 cut(s) 20
EcoRV GATATC 1 cut(s) 351
EcoT38I GRGCYC 1 cut(s) 166
FaeI CATG 4 cut(s) 59, 160, 265, 310
FaiI YATR 6 cut(s) 15, 57, 158, 263, 308, 321
FalI AAGNNNNNCTT 2 cut(s) 80, 112
FaqI GGGAC 1 cut(s) 302
FatI CATG 4 cut(s) 55, 156, 261, 306
FriOI GRGCYC 1 cut(s) 166
FspBI CTAG 2 cut(s) 27, 42
GsaI CCCAGC 1 cut(s) 389
HaeIII GGCC 1 cut(s) 22
Hin1II CATG 4 cut(s) 59, 160, 265, 310
HincII GTYRAC 1 cut(s) 217
HindII GTYRAC 1 cut(s) 217
HinfI GANTC 1 cut(s) 171
HphI GGTGA 1 cut(s) 401
Hpy166II GTNNAC 1 cut(s) 217
Hpy188I TCNGA 1 cut(s) 464
Hpy188III TCNNGA 3 cut(s) 175, 307, 416
Hpy8I GTNNAC 1 cut(s) 217
HpyAV CCTTC 2 cut(s) 33, 106
HpyCH4III ACNGT 1 cut(s) 446
HpyCH4V TGCA 2 cut(s) 209, 377
HpyF10VI GCNNNNNNNGC 2 cut(s) 76, 161
HpyF3I CTNAG 1 cut(s) 121
Hsp92II CATG 4 cut(s) 59, 160, 265, 310
LguI GCTCTTC 1 cut(s) 180
LpnPI CCDG 2 cut(s) 108, 371
LweI GCATC 1 cut(s) 124
MaeI CTAG 2 cut(s) 27, 42
MboII GAAGA 2 cut(s) 197, 382
MhlI GDGCHC 1 cut(s) 166
MluCI AATT 2 cut(s) 192, 394
MnlI CCTC 6 cut(s) 11, 20, 69, 153, 292, 421
MroXI GAANNNNTTC 1 cut(s) 314
MseI TTAA 4 cut(s) 62, 87, 195, 366
Mva1269I GAATGC 1 cut(s) 314
MwoI GCNNNNNNNGC 2 cut(s) 76, 161
NlaIII CATG 4 cut(s) 59, 160, 265, 310
NlaIV GGNNCC 1 cut(s) 235
PagI TCATGA 1 cut(s) 306
PciSI GCTCTTC 1 cut(s) 180
PcsI WCGNNNNNNNCGW 1 cut(s) 105
PctI GAATGC 1 cut(s) 314
PdmI GAANNNNTTC 1 cut(s) 314
PfeI GAWTC 1 cut(s) 171
PspFI CCCAGC 1 cut(s) 385
PspN4I GGNNCC 1 cut(s) 235
PspPI GGNCC 1 cut(s) 20
RsaI GTAC 1 cut(s) 452
RsaNI GTAC 1 cut(s) 451
SapI GCTCTTC 1 cut(s) 180
SaqAI TTAA 4 cut(s) 62, 87, 195, 366
Sau96I GGNCC 1 cut(s) 20
ScaI AGTACT 1 cut(s) 452
SduI GDGCHC 1 cut(s) 166
SetI ASST 2 cut(s) 43, 387
SfaNI GCATC 1 cut(s) 124
Sse9I AATT 2 cut(s) 192, 394
SspMI CTAG 2 cut(s) 27, 42
TaaI ACNGT 1 cut(s) 446
TaqI TCGA 3 cut(s) 99, 108, 174
TaqII GACCGA 1 cut(s) 453
TasI AATT 2 cut(s) 192, 394
TatI WGTACW 1 cut(s) 450
TfiI GAWTC 1 cut(s) 171
Tru1I TTAA 4 cut(s) 62, 87, 195, 366
Tru9I TTAA 4 cut(s) 62, 87, 195, 366
TscAI CASTG 2 cut(s) 118, 406
TspDTI ATGAA 1 cut(s) 323
TspGWI ACGGA 1 cut(s) 240
TspRI CASTG 2 cut(s) 118, 406
XapI RAATTY 1 cut(s) 394
XcmI CCANNNNNNNNNTGG 1 cut(s) 338
XmnI GAANNNNTTC 1 cut(s) 314
XspI CTAG 2 cut(s) 27, 42
ZrmI AGTACT 1 cut(s) 452
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.