AT3G51040

Protein of unknown function (DUF778)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0005598
Physical Location & Seq
Reverse (-)
18952073 .. 18953801
1729 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G51040.3

Sequence Viewer

Length: 696 bp
ATGGGAGAGACAGCAACAGATTCAGAACATCGCATGATGATCGGATTATCGGATCCTATGAAAATTGATCCCAAAAGAGACCGGTTTCCATGCTGCATTGTTTGGACACCTCTTCCCTTCATTTCATGGCTGGTTCCCTTCATCGGCCACGTCGGTATCTGCAGAGAAGACGGTGTCATCCTCGACTTTGCAGGACCTAATTTTGTCTGTGTTGATAATTTTGCATTTGGAGCTGTTTCTCGATATATTCAAATCAACAAAGAAATGGAGTCATCTCGTTCTTCCAGTTCTGGTATGTTCAATGGTGAGAGGAGATATGAGCAAGAAGAAGATAGTCATGAGAAAGAGCCAACATGGGATGATGCACTGAGAAAAAGCACGCAAGAGTACCAACATCACTCATATAACATACTCACATGCAACTGTCATTCCTTTGTAGCCAACAACCTGAACCGTCTATCTATAAAGTCAGGCGGATGGAACGTGGTGAATCTCGCGACACTAGTGCTTTTCAAGGGACGTTGGGTGAACAAAACGGCTATAGTAAAGTCCTTGTTGCCTCCACTCATAGTCTACACTATAGGAATCTTACTCGGTGGTTGGACTTTCATTGCCTCCTGCTCGATTCTAGTGGTTTTGCTTACCGGGTGGTTCATCATTGGAACGTATTGTTTCAAGAAACTGATTCAGCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04016 map04016
Pfam Domains
Protein Families
DUF778

Protein Analysis

231

Amino Acids

26.16

Weight (kDa)

6.99

Isoelectric Point (pI)

38.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RTE1 PF05608 23 - 173 4.1e-67 RTE1-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 573
AccII CGCG 1 cut(s) 497
AciI CCGC 1 cut(s) 474
AclWI GGATC 3 cut(s) 47, 60, 62
AcoI YGGCCR 1 cut(s) 145
AfaI GTAC 1 cut(s) 389
AfiI CCNNNNNNNGG 1 cut(s) 143
AgeI ACCGGT 1 cut(s) 81
AgsI TTSAA 4 cut(s) 251, 301, 514, 676
AhlI ACTAGT 1 cut(s) 502
AjiI CACGTC 1 cut(s) 151
AluBI AGCT 2 cut(s) 233, 691
AluI AGCT 2 cut(s) 233, 691
Alw26I GTCTC 2 cut(s) 2, 72
AlwI GGATC 3 cut(s) 47, 60, 62
AoxI GGCC 1 cut(s) 145
ApeKI GCWGC 1 cut(s) 93
AsiGI ACCGGT 1 cut(s) 81
AspS9I GGNCC 1 cut(s) 194
AsuC2I CCSGG 1 cut(s) 646
AsuHPI GGTGA 3 cut(s) 317, 499, 538
AvaII GGWCC 1 cut(s) 194
BaeI ACNNNNGTAYC 2 cut(s) 139, 172
BamHI GGATCC 1 cut(s) 52
BbsI GAAGAC 1 cut(s) 174
BbvI GCAGC 1 cut(s) 80
BccI CCATC 1 cut(s) 471
BceAI ACGGC 1 cut(s) 552
BcgI CGANNNNNNTGC 4 cut(s) 22, 56, 487, 521
BcnI CCSGG 1 cut(s) 646
BcoDI GTCTC 2 cut(s) 2, 72
BcuI ACTAGT 1 cut(s) 502
BfaI CTAG 2 cut(s) 503, 629
BfmI CTRYAG 4 cut(s) 160, 540, 579, 692
BisI GCNGC 1 cut(s) 94
BlsI GCNGC 1 cut(s) 95
Bme1390I CCNGG 1 cut(s) 646
Bme18I GGWCC 1 cut(s) 194
BmgBI CACGTC 1 cut(s) 151
BmgT120I GGNCC 1 cut(s) 194
BmiI GGNNCC 2 cut(s) 54, 135
BmrFI CCNGG 1 cut(s) 646
BmsI GCATC 1 cut(s) 352
BpiI GAAGAC 1 cut(s) 174
BpuMI CCSGG 1 cut(s) 646
BsaI GGTCTC 1 cut(s) 72
BsaWI WCCGGW 1 cut(s) 81
Bsc4I CCNNNNNNNGG 1 cut(s) 143
Bse118I RCCGGY 1 cut(s) 81
Bse1I ACTGG 1 cut(s) 285
Bse3DI GCAATG 1 cut(s) 609
BseGI GGATG 3 cut(s) 177, 364, 482
BseLI CCNNNNNNNGG 1 cut(s) 143
BseMI GCAATG 1 cut(s) 609
BseMII CTCAG 1 cut(s) 359
BseNI ACTGG 1 cut(s) 285
BseRI GAGGAG 1 cut(s) 325
BseXI GCAGC 1 cut(s) 80
Bsh1236I CGCG 1 cut(s) 497
BshFI GGCC 1 cut(s) 147
BshTI ACCGGT 1 cut(s) 81
BsiSI CCGG 2 cut(s) 82, 645
BslFI GGGAC 1 cut(s) 531
BslI CCNNNNNNNGG 1 cut(s) 143
BsmAI GTCTC 2 cut(s) 2, 72
BsmFI GGGAC 1 cut(s) 531
BsnI GGCC 1 cut(s) 147
Bso31I GGTCTC 1 cut(s) 72
Bsp143I GATC 3 cut(s) 39, 52, 67
Bsp68I TCGCGA 1 cut(s) 497
BspACI CCGC 1 cut(s) 474
BspANI GGCC 1 cut(s) 147
BspCNI CTCAG 1 cut(s) 360
BspFNI CGCG 1 cut(s) 497
BspHI TCATGA 1 cut(s) 337
BspLI GGNNCC 2 cut(s) 54, 135
BspMAI CTGCAG 1 cut(s) 164
BspPI GGATC 3 cut(s) 47, 60, 62
BspTNI GGTCTC 1 cut(s) 72
BsrDI GCAATG 1 cut(s) 609
BsrFI RCCGGY 1 cut(s) 81
BsrI ACTGG 1 cut(s) 285
BssAI RCCGGY 1 cut(s) 81
BssMI GATC 3 cut(s) 39, 52, 67
Bst4CI ACNGT 3 cut(s) 173, 425, 455
Bst6I CTCTTC 1 cut(s) 117
BstC8I GCNNGC 1 cut(s) 380
BstDEI CTNAG 1 cut(s) 368
BstF5I GGATG 3 cut(s) 177, 364, 482
BstFNI CGCG 1 cut(s) 497
BstKTI GATC 3 cut(s) 42, 55, 70
BstMAI GTCTC 2 cut(s) 2, 72
BstMBI GATC 3 cut(s) 39, 52, 67
BstMWI GCNNNNNNNGC 1 cut(s) 230
BstNSI RCATGY 1 cut(s) 420
BstSCI CCNGG 1 cut(s) 644
BstSFI CTRYAG 4 cut(s) 160, 540, 579, 692
BstUI CGCG 1 cut(s) 497
BstV1I GCAGC 1 cut(s) 80
BstV2I GAAGAC 1 cut(s) 174
BstX2I RGATCY 1 cut(s) 52
BstYI RGATCY 1 cut(s) 52
BsuRI GGCC 1 cut(s) 147
BtgZI GCGATG 1 cut(s) 14
BtrI CACGTC 1 cut(s) 151
BtsCI GGATG 3 cut(s) 177, 364, 482
BtsIMutI CAGTG 1 cut(s) 365
BtuMI TCGCGA 1 cut(s) 497
Cac8I GCNNGC 1 cut(s) 380
CciI TCATGA 1 cut(s) 337
Cfr10I RCCGGY 1 cut(s) 81
Cfr13I GGNCC 1 cut(s) 194
Csp6I GTAC 1 cut(s) 388
CspAI ACCGGT 1 cut(s) 81
CviAII CATG 6 cut(s) 34, 90, 126, 338, 354, 417
CviJI RGCY 7 cut(s) 130, 147, 233, 349, 440, 539, 691
CviKI_1 RGCY 7 cut(s) 130, 147, 233, 349, 440, 539, 691
CviQI GTAC 1 cut(s) 388
DdeI CTNAG 1 cut(s) 368
DpnI GATC 3 cut(s) 41, 54, 69
DpnII GATC 3 cut(s) 39, 52, 67
EaeI YGGCCR 1 cut(s) 145
Eam1104I CTCTTC 1 cut(s) 117
EarI CTCTTC 1 cut(s) 117
EciI GGCGGA 1 cut(s) 489
Eco31I GGTCTC 1 cut(s) 72
Eco47I GGWCC 1 cut(s) 194
EcoO109I RGGNCCY 1 cut(s) 194
FaeI CATG 6 cut(s) 37, 93, 129, 341, 357, 420
FaqI GGGAC 1 cut(s) 531
FatI CATG 6 cut(s) 33, 89, 125, 337, 353, 416
FblI GTMKAC 1 cut(s) 573
Fnu4HI GCNGC 1 cut(s) 94
FokI GGATG 3 cut(s) 164, 371, 489
Fsp4HI GCNGC 1 cut(s) 94
FspBI CTAG 2 cut(s) 503, 629
GluI GCNGC 1 cut(s) 94
HaeIII GGCC 1 cut(s) 147
HapII CCGG 2 cut(s) 82, 645
Hin1II CATG 6 cut(s) 37, 93, 129, 341, 357, 420
HinfI GANTC 6 cut(s) 20, 269, 490, 585, 625, 685
HpaII CCGG 2 cut(s) 82, 645
HphI GGTGA 3 cut(s) 317, 499, 538
Hpy166II GTNNAC 2 cut(s) 529, 574
Hpy188I TCNGA 3 cut(s) 25, 44, 52
Hpy188III TCNNGA 4 cut(s) 240, 338, 496, 676
Hpy8I GTNNAC 2 cut(s) 529, 574
Hpy99I CGWCG 1 cut(s) 155
HpyAV CCTTC 2 cut(s) 127, 148
HpyCH4III ACNGT 3 cut(s) 173, 425, 455
HpyCH4IV ACGT 4 cut(s) 150, 483, 520, 665
HpyCH4V TGCA 6 cut(s) 96, 162, 191, 224, 365, 420
HpyF10VI GCNNNNNNNGC 1 cut(s) 230
HpyF3I CTNAG 1 cut(s) 368
HpySE526I ACGT 4 cut(s) 150, 483, 520, 665
Hsp92II CATG 6 cut(s) 37, 93, 129, 341, 357, 420
Kzo9I GATC 3 cut(s) 39, 52, 67
LmnI GCTCC 1 cut(s) 230
LpnPI CCDG 9 cut(s) 95, 116, 177, 276, 298, 456, 461, 631, 658
Lsp1109I GCAGC 1 cut(s) 80
LweI GCATC 1 cut(s) 352
MaeI CTAG 2 cut(s) 503, 629
MaeII ACGT 4 cut(s) 150, 483, 520, 665
MalI GATC 3 cut(s) 41, 54, 69
MboI GATC 3 cut(s) 39, 52, 67
MboII GAAGA 5 cut(s) 104, 179, 273, 338, 341
MflI RGATCY 1 cut(s) 52
MluCI AATT 3 cut(s) 63, 199, 217
MlyI GAGTC 1 cut(s) 278
MmeI TCCRAC 1 cut(s) 581
MnlI CCTC 5 cut(s) 120, 191, 303, 570, 625
MspA1I CMGCKG 1 cut(s) 691
MspI CCGG 2 cut(s) 82, 645
MspR9I CCNGG 1 cut(s) 646
MvnI CGCG 1 cut(s) 497
MwoI GCNNNNNNNGC 1 cut(s) 230
NciI CCSGG 1 cut(s) 646
NdeII GATC 3 cut(s) 39, 52, 67
NlaIII CATG 6 cut(s) 37, 93, 129, 341, 357, 420
NlaIV GGNNCC 2 cut(s) 54, 135
NruI TCGCGA 1 cut(s) 497
NspI RCATGY 1 cut(s) 420
PagI TCATGA 1 cut(s) 337
PfeI GAWTC 5 cut(s) 20, 490, 585, 625, 685
PflFI GACNNNGTC 1 cut(s) 173
PinAI ACCGGT 1 cut(s) 81
PkrI GCNGC 1 cut(s) 95
PleI GAGTC 1 cut(s) 277
PpsI GAGTC 1 cut(s) 277
PpuMI RGGWCCY 1 cut(s) 194
Psp5II RGGWCCY 1 cut(s) 194
PspN4I GGNNCC 2 cut(s) 54, 135
PspPI GGNCC 1 cut(s) 194
PspPPI RGGWCCY 1 cut(s) 194
PstI CTGCAG 1 cut(s) 164
PsuI RGATCY 1 cut(s) 52
PsyI GACNNNGTC 1 cut(s) 173
PvuII CAGCTG 1 cut(s) 691
RruI TCGCGA 1 cut(s) 497
RsaI GTAC 1 cut(s) 389
RsaNI GTAC 1 cut(s) 388
SatI GCNGC 1 cut(s) 94
Sau3AI GATC 3 cut(s) 39, 52, 67
Sau96I GGNCC 1 cut(s) 194
SchI GAGTC 1 cut(s) 278
ScrFI CCNGG 1 cut(s) 646
SetI ASST 9 cut(s) 112, 153, 199, 235, 450, 486, 523, 668, 693
SfaNI GCATC 1 cut(s) 352
SfcI CTRYAG 4 cut(s) 160, 540, 579, 692
SinI GGWCC 1 cut(s) 194
SpeI ACTAGT 1 cut(s) 502
Sse9I AATT 3 cut(s) 63, 199, 217
SsiI CCGC 1 cut(s) 474
SspMI CTAG 2 cut(s) 503, 629
StyD4I CCNGG 1 cut(s) 644
TaaI ACNGT 3 cut(s) 173, 425, 455
TaiI ACGT 4 cut(s) 153, 486, 523, 668
TaqI TCGA 3 cut(s) 183, 241, 623
TasI AATT 3 cut(s) 63, 199, 217
TfiI GAWTC 5 cut(s) 20, 490, 585, 625, 685
TscAI CASTG 1 cut(s) 372
TseI GCWGC 1 cut(s) 93
TspDTI ATGAA 6 cut(s) 74, 109, 114, 130, 598, 643
TspRI CASTG 1 cut(s) 372
Tth111I GACNNNGTC 1 cut(s) 173
VpaK11BI GGWCC 1 cut(s) 194
XceI RCATGY 1 cut(s) 420
XmiI GTMKAC 1 cut(s) 573
XspI CTAG 2 cut(s) 503, 629
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.