MD15G1250900.v1.1

RTE1-HOMOLOG-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0005598
Physical Location & Seq
Forward (+)
21105843 .. 21116897
11055 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1250900.v1.1.491

Sequence Viewer

Length: 693 bp
ATGGAAACAAGTGCAGGCCCCGAGCACAACTTGATGATTGATGGAAGTGATTCACCGACATTGCAAATTGACCCCAAAAGATCCCGGTTCCCATGCTGTATTGTGTGGACACCCCTTCCTGTTATTTCATGGTTGATTCCTTTTGTTGGCCACATTGGCATATGCAGAGAAGATGGTGTGATATTGGACTTTGCGGGGCCCAATTTCGTGTGCGTGGATAACTTTGCTTTTGGAGCAGCGACTCGCTACATCCAAATAAGCAAAGAAAAGTGTTGCGCTATTCCCGATCCGTCTGAGTACCTAAGTGAGGATCAATATGGGCAGAATGAACGTGGAAGCGACATAAAGACATGGGATGATGCGCTCCGGAAGGGCACTCAGGAATTCCAACACCGAGCTTACAACCTCTTTACCTGCAATTGCCACTCCTTTGTAGCCAACAACCTAAACAGGTTAGGCTTTCGGTCTGGTGGGTGGAATGTGGTGAACCTTGCAGCTCTGATCTTTCTCAAGGGTCAGTGGGTGAGCACAGCAGCCGTGGTGCAGTCCTTACTACCGTTTTTAATAACATTCTGTCTCGGAGTTGCATTAGCAGGTTCAACCTTCCTAAAGTACTTAGGTTTCTTCACCGCCCTTCTTGTCGGTTGGTTTCTTCTTGGTACGTACTGTTTCAAGAGTTTGATCCAGTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04016 map04016
Pfam Domains
Protein Families
DUF778

Protein Analysis

231

Amino Acids

25.58

Weight (kDa)

6.06

Isoelectric Point (pI)

31.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RTE1 PF05608 24 - 172 7.4e-71 RTE1-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 422, 584
AccIII TCCGGA 1 cut(s) 366
AciI CCGC 2 cut(s) 194, 630
AclWI GGATC 4 cut(s) 75, 281, 318, 676
AcoI YGGCCR 1 cut(s) 148
AcsI RAATTY 1 cut(s) 383
AfaI GTAC 4 cut(s) 299, 614, 661, 665
AfiI CCNNNNNNNGG 2 cut(s) 146, 307
AgsI TTSAA 2 cut(s) 600, 673
AluBI AGCT 2 cut(s) 398, 497
AluI AGCT 2 cut(s) 398, 497
Alw21I GWGCWC 2 cut(s) 27, 530
Alw26I GTCTC 1 cut(s) 581
AlwI GGATC 4 cut(s) 75, 281, 318, 676
Ama87I CYCGRG 1 cut(s) 20
Aor13HI TCCGGA 1 cut(s) 366
AoxI GGCC 3 cut(s) 16, 148, 197
ApaI GGGCCC 1 cut(s) 201
ApeKI GCWGC 3 cut(s) 236, 494, 533
ApoI RAATTY 1 cut(s) 383
Asp700I GAANNNNTTC 1 cut(s) 49
AspLEI GCGC 2 cut(s) 278, 364
AspS9I GGNCC 3 cut(s) 17, 197, 198
AsuC2I CCSGG 1 cut(s) 85
AsuHPI GGTGA 4 cut(s) 45, 496, 535, 619
AvaI CYCGRG 1 cut(s) 20
BaeGI GKGCMC 2 cut(s) 201, 377
BalI TGGCCA 1 cut(s) 150
BanII GRGCYC 1 cut(s) 201
Bbv12I GWGCWC 2 cut(s) 27, 530
BbvI GCAGC 3 cut(s) 248, 506, 545
BccI CCATC 2 cut(s) 35, 167
BceAI ACGGC 1 cut(s) 521
BcnI CCSGG 1 cut(s) 85
BcoDI GTCTC 1 cut(s) 581
BfuAI ACCTGC 2 cut(s) 422, 584
BglI GCCNNNNNGGC 1 cut(s) 156
BisI GCNGC 3 cut(s) 237, 495, 534
BlsI GCNGC 3 cut(s) 238, 496, 535
BmcAI AGTACT 1 cut(s) 614
Bme1390I CCNGG 1 cut(s) 85
BmeT110I CYCGRG 1 cut(s) 20
BmgT120I GGNCC 3 cut(s) 17, 197, 198
BmiI GGNNCC 4 cut(s) 19, 89, 198, 199
BmrFI CCNGG 1 cut(s) 85
BmsI GCATC 1 cut(s) 349
BpuEI CTTGAG 1 cut(s) 494
BpuMI CCSGG 1 cut(s) 85
BsaAI YACGTR 1 cut(s) 663
BsaJI CCNNGG 1 cut(s) 537
BsaWI WCCGGW 1 cut(s) 366
Bsc4I CCNNNNNNNGG 2 cut(s) 146, 307
Bse1I ACTGG 1 cut(s) 685
Bse3DI GCAATG 1 cut(s) 59
BseAI TCCGGA 1 cut(s) 366
BseDI CCNNGG 1 cut(s) 537
BseGI GGATG 2 cut(s) 249, 361
BseLI CCNNNNNNNGG 2 cut(s) 146, 307
BseMI GCAATG 1 cut(s) 59
BseMII CTCAG 2 cut(s) 285, 392
BseNI ACTGG 1 cut(s) 685
BseSI GKGCMC 2 cut(s) 201, 377
BseXI GCAGC 3 cut(s) 248, 506, 545
BsgI GTGCAG 2 cut(s) 33, 563
BshFI GGCC 3 cut(s) 18, 150, 199
BsiHKAI GWGCWC 2 cut(s) 27, 530
BsiHKCI CYCGRG 1 cut(s) 20
BsiSI CCGG 2 cut(s) 85, 367
BslI CCNNNNNNNGG 2 cut(s) 146, 307
BsmAI GTCTC 1 cut(s) 581
BsnI GGCC 3 cut(s) 18, 150, 199
BsoBI CYCGRG 1 cut(s) 20
Bsp120I GGGCCC 1 cut(s) 197
Bsp1286I GDGCHC 4 cut(s) 27, 201, 377, 530
Bsp13I TCCGGA 1 cut(s) 366
Bsp143I GATC 5 cut(s) 80, 286, 310, 501, 681
BspACI CCGC 2 cut(s) 194, 630
BspANI GGCC 3 cut(s) 18, 150, 199
BspCNI CTCAG 2 cut(s) 286, 391
BspEI TCCGGA 1 cut(s) 366
BspLI GGNNCC 4 cut(s) 19, 89, 198, 199
BspMI ACCTGC 2 cut(s) 422, 584
BspPI GGATC 4 cut(s) 75, 281, 318, 676
BsrDI GCAATG 1 cut(s) 59
BsrI ACTGG 1 cut(s) 685
BssECI CCNNGG 1 cut(s) 537
BssMI GATC 5 cut(s) 80, 286, 310, 501, 681
Bst4CI ACNGT 2 cut(s) 558, 668
BstBAI YACGTR 1 cut(s) 663
BstC8I GCNNGC 1 cut(s) 16
BstDEI CTNAG 4 cut(s) 294, 302, 378, 616
BstDSI CCRYGG 1 cut(s) 537
BstENI CCTNNNNNAGG 1 cut(s) 305
BstF5I GGATG 2 cut(s) 249, 361
BstHHI GCGC 2 cut(s) 278, 364
BstKTI GATC 5 cut(s) 83, 289, 313, 504, 684
BstMAI GTCTC 1 cut(s) 581
BstMBI GATC 5 cut(s) 80, 286, 310, 501, 681
BstMWI GCNNNNNNNGC 2 cut(s) 156, 233
BstSCI CCNGG 1 cut(s) 83
BstSLI GKGCMC 2 cut(s) 201, 377
BstSNI TACGTA 1 cut(s) 663
BstV1I GCAGC 3 cut(s) 248, 506, 545
BstX2I RGATCY 1 cut(s) 80
BstYI RGATCY 1 cut(s) 80
BsuRI GGCC 3 cut(s) 18, 150, 199
BtgI CCRYGG 1 cut(s) 537
BtsCI GGATG 2 cut(s) 249, 361
BtsIMutI CAGTG 1 cut(s) 524
BveI ACCTGC 2 cut(s) 422, 584
Cac8I GCNNGC 1 cut(s) 16
CfoI GCGC 2 cut(s) 278, 364
Cfr13I GGNCC 3 cut(s) 17, 197, 198
Csp6I GTAC 4 cut(s) 298, 613, 660, 664
CviAII CATG 3 cut(s) 93, 129, 351
CviJI RGCY 8 cut(s) 18, 150, 199, 398, 437, 459, 497, 536
CviKI_1 RGCY 8 cut(s) 18, 150, 199, 398, 437, 459, 497, 536
CviQI GTAC 4 cut(s) 298, 613, 660, 664
DdeI CTNAG 4 cut(s) 294, 302, 378, 616
DpnI GATC 5 cut(s) 82, 288, 312, 503, 683
DpnII GATC 5 cut(s) 80, 286, 310, 501, 681
EaeI YGGCCR 1 cut(s) 148
Eco105I TACGTA 1 cut(s) 663
Eco24I GRGCYC 1 cut(s) 201
Eco88I CYCGRG 1 cut(s) 20
EcoNI CCTNNNNNAGG 1 cut(s) 305
EcoO109I RGGNCCY 2 cut(s) 17, 197
EcoRI GAATTC 1 cut(s) 383
EcoT38I GRGCYC 1 cut(s) 201
FaeI CATG 3 cut(s) 96, 132, 354
FaiI YATR 7 cut(s) 94, 130, 161, 163, 318, 344, 352
FatI CATG 3 cut(s) 92, 128, 350
FauI CCCGC 1 cut(s) 187
FauNDI CATATG 1 cut(s) 161
Fnu4HI GCNGC 3 cut(s) 237, 495, 534
FokI GGATG 2 cut(s) 236, 368
FriOI GRGCYC 1 cut(s) 201
Fsp4HI GCNGC 3 cut(s) 237, 495, 534
GlaI GCGC 2 cut(s) 277, 363
GluI GCNGC 3 cut(s) 237, 495, 534
HaeIII GGCC 3 cut(s) 18, 150, 199
HapII CCGG 2 cut(s) 85, 367
HhaI GCGC 2 cut(s) 278, 364
Hin1II CATG 3 cut(s) 96, 132, 354
Hin6I GCGC 2 cut(s) 276, 362
HinP1I GCGC 2 cut(s) 276, 362
HinfI GANTC 3 cut(s) 50, 136, 241
HpaII CCGG 2 cut(s) 85, 367
HphI GGTGA 4 cut(s) 45, 496, 535, 619
Hpy166II GTNNAC 2 cut(s) 108, 487
Hpy188I TCNGA 3 cut(s) 295, 501, 581
Hpy188III TCNNGA 4 cut(s) 284, 367, 380, 673
Hpy8I GTNNAC 2 cut(s) 108, 487
HpyAV CCTTC 4 cut(s) 125, 364, 613, 644
HpyCH4III ACNGT 2 cut(s) 558, 668
HpyCH4IV ACGT 2 cut(s) 331, 662
HpyCH4V TGCA 7 cut(s) 14, 64, 165, 417, 494, 544, 587
HpyF10VI GCNNNNNNNGC 2 cut(s) 156, 233
HpyF3I CTNAG 4 cut(s) 294, 302, 378, 616
HpySE526I ACGT 2 cut(s) 331, 662
Hsp92II CATG 3 cut(s) 96, 132, 354
HspAI GCGC 2 cut(s) 276, 362
Kpn2I TCCGGA 1 cut(s) 366
Kzo9I GATC 5 cut(s) 80, 286, 310, 501, 681
LmnI GCTCC 2 cut(s) 233, 369
LpnPI CCDG 8 cut(s) 98, 132, 365, 380, 427, 436, 453, 579
Lsp1109I GCAGC 3 cut(s) 248, 506, 545
LweI GCATC 1 cut(s) 349
MaeII ACGT 2 cut(s) 331, 662
MalI GATC 5 cut(s) 82, 288, 312, 503, 683
MboI GATC 5 cut(s) 80, 286, 310, 501, 681
MboII GAAGA 3 cut(s) 182, 616, 644
MfeI CAATTG 1 cut(s) 418
MflI RGATCY 1 cut(s) 80
MhlI GDGCHC 4 cut(s) 27, 201, 377, 530
MlsI TGGCCA 1 cut(s) 150
MluCI AATT 4 cut(s) 66, 202, 383, 418
MluNI TGGCCA 1 cut(s) 150
MlyI GAGTC 1 cut(s) 235
MmeI TCCRAC 1 cut(s) 412
MnlI CCTC 2 cut(s) 301, 416
Mox20I TGGCCA 1 cut(s) 150
MroI TCCGGA 1 cut(s) 366
MroXI GAANNNNTTC 1 cut(s) 49
MscI TGGCCA 1 cut(s) 150
MseI TTAA 1 cut(s) 563
Msp20I TGGCCA 1 cut(s) 150
MspI CCGG 2 cut(s) 85, 367
MspR9I CCNGG 1 cut(s) 85
MunI CAATTG 1 cut(s) 418
MwoI GCNNNNNNNGC 2 cut(s) 156, 233
NciI CCSGG 1 cut(s) 85
NdeI CATATG 1 cut(s) 161
NdeII GATC 5 cut(s) 80, 286, 310, 501, 681
NlaIII CATG 3 cut(s) 96, 132, 354
NlaIV GGNNCC 4 cut(s) 19, 89, 198, 199
PdmI GAANNNNTTC 1 cut(s) 49
PfeI GAWTC 2 cut(s) 50, 136
PkrI GCNGC 3 cut(s) 238, 496, 535
PleI GAGTC 1 cut(s) 235
PpsI GAGTC 1 cut(s) 235
Ppu21I YACGTR 1 cut(s) 663
PspN4I GGNNCC 4 cut(s) 19, 89, 198, 199
PspOMI GGGCCC 1 cut(s) 197
PspPI GGNCC 3 cut(s) 17, 197, 198
PsuI RGATCY 1 cut(s) 80
RsaI GTAC 4 cut(s) 299, 614, 661, 665
RsaNI GTAC 4 cut(s) 298, 613, 660, 664
SaqAI TTAA 1 cut(s) 563
SatI GCNGC 3 cut(s) 237, 495, 534
Sau3AI GATC 5 cut(s) 80, 286, 310, 501, 681
Sau96I GGNCC 3 cut(s) 17, 197, 198
ScaI AGTACT 1 cut(s) 614
SchI GAGTC 1 cut(s) 235
ScrFI CCNGG 1 cut(s) 85
SduI GDGCHC 4 cut(s) 27, 201, 377, 530
SfaNI GCATC 1 cut(s) 349
SmlI CTYRAG 1 cut(s) 509
SmoI CTYRAG 1 cut(s) 509
SnaBI TACGTA 1 cut(s) 663
Sse9I AATT 4 cut(s) 66, 202, 383, 418
SsiI CCGC 2 cut(s) 194, 630
StyD4I CCNGG 1 cut(s) 83
TaaI ACNGT 2 cut(s) 558, 668
TaiI ACGT 2 cut(s) 334, 665
TaqII GACCGA 1 cut(s) 453
TasI AATT 4 cut(s) 66, 202, 383, 418
TatI WGTACW 1 cut(s) 612
TfiI GAWTC 2 cut(s) 50, 136
Tru1I TTAA 1 cut(s) 563
Tru9I TTAA 1 cut(s) 563
TscAI CASTG 1 cut(s) 524
TseI GCWGC 3 cut(s) 236, 494, 533
TspDTI ATGAA 2 cut(s) 117, 342
TspGWI ACGGA 1 cut(s) 279
TspRI CASTG 1 cut(s) 524
XagI CCTNNNNNAGG 1 cut(s) 305
XapI RAATTY 1 cut(s) 383
XmnI GAANNNNTTC 1 cut(s) 49
ZrmI AGTACT 1 cut(s) 614
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.