Prupe.7G163300_v2.0.a1

RTE1-HOMOLOG-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp07
OG0005598
Physical Location & Seq
Reverse (-)
16833931 .. 16836156
2226 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.7G163300.2

Sequence Viewer

Length: 747 bp
ATGGAAAATCTCTTTTTTGAACAAGAGGAGTTAATTCTTCCAATTTTGGCAATGGAAACAAGTGCAAGCCCTGAGCACAACTTGATGATCGATGGAAGTGCTTCGCCAACTCTGCAAATTGACCCTAGAAGAGCCCGGTTTCCATGCTGCATTGTGTGGACACCCCTTCCAGTTATTTCATGGTTGATTCCTTTCATTGGCCACATTGGCATATGCAGAGAAGATGGTGTGATTTTGGACTTTGCTGGGCCCAATTTTGTGTGTGTGGATAACTTTGCTTTCGGAGCAGCGACTCGCTACATCCAAATAAGCAAAGAAAAGCAGTGTCACTCTATTCCCAATCCATCTGTGTGTCAAAGCGAGGATCAATACAGACAGGATGAACCTGGAAGAGACATAATGACATGGGATGATGCCCTTCGGAAGGGCACGCAGGAATTCCAACACCGATCTTATAATATTCTTACCTGCAATTGCCACTCCTTTGTAGCCAACAACCTAAACAGGTTAGGCTTTTGTTCTGGTGGGTGGAATGTGGTGAACCTTGCAGCTCTGATCTTCCTCAAGGGTAAATGGGTGAGCACGGCATCCATGGTGCGGTCCTTTTTACCATTTTTAATAGCATTCGGTCTTGGAGTTGCATTTGCAGGTTCAACCTTCCTAACTTACTTAGGATTCTTCACGGCCTTTCTTGTTGGATGGTTTCTTCTTGGTACTTACTGTTTCAAGAGTTTGATCCATTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04016 map04016
Pfam Domains
Protein Families
DUF778

Protein Analysis

249

Amino Acids

27.85

Weight (kDa)

5.84

Isoelectric Point (pI)

41.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 456
Acc36I ACCTGC 2 cut(s) 476, 638
AciI CCGC 1 cut(s) 598
AclWI GGATC 2 cut(s) 372, 730
AcoI YGGCCR 1 cut(s) 199
AcsI RAATTY 1 cut(s) 437
AfaI GTAC 1 cut(s) 715
AfiI CCNNNNNNNGG 3 cut(s) 197, 424, 597
AgsI TTSAA 3 cut(s) 20, 654, 727
AjnI CCWGG 1 cut(s) 385
AluBI AGCT 1 cut(s) 551
AluI AGCT 1 cut(s) 551
Alw21I GWGCWC 2 cut(s) 78, 584
Alw26I GTCTC 1 cut(s) 387
AlwI GGATC 2 cut(s) 372, 730
AoxI GGCC 3 cut(s) 199, 248, 684
ApaI GGGCCC 1 cut(s) 252
ApeKI GCWGC 3 cut(s) 147, 287, 548
ApoI RAATTY 1 cut(s) 437
Asp700I GAANNNNTTC 1 cut(s) 100
AspS9I GGNCC 3 cut(s) 248, 249, 600
AsuC2I CCSGG 1 cut(s) 136
AsuHPI GGTGA 2 cut(s) 550, 589
AvaII GGWCC 1 cut(s) 600
BaeGI GKGCMC 2 cut(s) 252, 431
BalI TGGCCA 1 cut(s) 201
BanII GRGCYC 2 cut(s) 136, 252
Bbv12I GWGCWC 2 cut(s) 78, 584
BbvI GCAGC 3 cut(s) 134, 299, 560
BccI CCATC 4 cut(s) 86, 218, 352, 693
BceAI ACGGC 2 cut(s) 600, 699
BcgI CGANNNNNNTGC 2 cut(s) 80, 114
BciT130I CCWGG 1 cut(s) 387
BcnI CCSGG 1 cut(s) 136
BcoDI GTCTC 1 cut(s) 387
BfaI CTAG 1 cut(s) 126
BfuAI ACCTGC 2 cut(s) 476, 638
BglI GCCNNNNNGGC 1 cut(s) 207
BisI GCNGC 3 cut(s) 148, 288, 549
BlsI GCNGC 3 cut(s) 149, 289, 550
Bme1390I CCNGG 2 cut(s) 136, 387
Bme18I GGWCC 1 cut(s) 600
BmgT120I GGNCC 3 cut(s) 248, 249, 600
BmiI GGNNCC 1 cut(s) 250
BmrFI CCNGG 2 cut(s) 136, 387
BmsI GCATC 2 cut(s) 403, 596
Bpu10I CCTNAGC 1 cut(s) 72
BpuEI CTTGAG 1 cut(s) 548
BpuMI CCSGG 1 cut(s) 136
Bsa29I ATCGAT 1 cut(s) 90
BsaJI CCNNGG 1 cut(s) 591
Bsc4I CCNNNNNNNGG 3 cut(s) 197, 424, 597
Bse1I ACTGG 1 cut(s) 170
Bse3DI GCAATG 1 cut(s) 57
BseBI CCWGG 1 cut(s) 387
BseCI ATCGAT 1 cut(s) 90
BseDI CCNNGG 1 cut(s) 591
BseGI GGATG 5 cut(s) 300, 385, 415, 587, 704
BseLI CCNNNNNNNGG 3 cut(s) 197, 424, 597
BseMI GCAATG 1 cut(s) 57
BseMII CTCAG 1 cut(s) 63
BseNI ACTGG 1 cut(s) 170
BseRI GAGGAG 1 cut(s) 41
BseSI GKGCMC 2 cut(s) 252, 431
BseXI GCAGC 3 cut(s) 134, 299, 560
BseYI CCCAGC 1 cut(s) 245
BshFI GGCC 3 cut(s) 201, 250, 686
BshVI ATCGAT 1 cut(s) 90
BsiHKAI GWGCWC 2 cut(s) 78, 584
BsiSI CCGG 1 cut(s) 136
BslI CCNNNNNNNGG 3 cut(s) 197, 424, 597
BsmAI GTCTC 1 cut(s) 387
BsmI GAATGC 1 cut(s) 623
BsnI GGCC 3 cut(s) 201, 250, 686
Bsp120I GGGCCC 1 cut(s) 248
Bsp1286I GDGCHC 5 cut(s) 78, 136, 252, 431, 584
Bsp143I GATC 5 cut(s) 87, 364, 449, 555, 735
Bsp19I CCATGG 1 cut(s) 591
BspACI CCGC 1 cut(s) 598
BspANI GGCC 3 cut(s) 201, 250, 686
BspCNI CTCAG 1 cut(s) 64
BspDI ATCGAT 1 cut(s) 90
BspLI GGNNCC 1 cut(s) 250
BspMI ACCTGC 2 cut(s) 476, 638
BspPI GGATC 2 cut(s) 372, 730
BspQI GCTCTTC 1 cut(s) 124
BsrDI GCAATG 1 cut(s) 57
BsrI ACTGG 1 cut(s) 170
BssECI CCNNGG 1 cut(s) 591
BssMI GATC 5 cut(s) 87, 364, 449, 555, 735
BssT1I CCWWGG 1 cut(s) 591
Bst2UI CCWGG 1 cut(s) 387
Bst4CI ACNGT 1 cut(s) 722
Bst6I CTCTTC 2 cut(s) 124, 385
BstC8I GCNNGC 2 cut(s) 67, 431
BstDEI CTNAG 2 cut(s) 72, 670
BstDSI CCRYGG 1 cut(s) 591
BstENI CCTNNNNNAGG 1 cut(s) 422
BstF5I GGATG 5 cut(s) 300, 385, 415, 587, 704
BstKTI GATC 5 cut(s) 90, 367, 452, 558, 738
BstMAI GTCTC 1 cut(s) 387
BstMBI GATC 5 cut(s) 87, 364, 449, 555, 735
BstMWI GCNNNNNNNGC 3 cut(s) 112, 207, 284
BstNI CCWGG 1 cut(s) 387
BstSCI CCNGG 2 cut(s) 134, 385
BstSLI GKGCMC 2 cut(s) 252, 431
BstV1I GCAGC 3 cut(s) 134, 299, 560
Bsu15I ATCGAT 1 cut(s) 90
BsuRI GGCC 3 cut(s) 201, 250, 686
BsuTUI ATCGAT 1 cut(s) 90
BtgI CCRYGG 1 cut(s) 591
BtsCI GGATG 5 cut(s) 300, 385, 415, 587, 704
BtsI GCAGTG 1 cut(s) 329
BtsIMutI CAGTG 1 cut(s) 329
BveI ACCTGC 2 cut(s) 476, 638
Cac8I GCNNGC 2 cut(s) 67, 431
Cfr13I GGNCC 3 cut(s) 248, 249, 600
ClaI ATCGAT 1 cut(s) 90
Csp6I GTAC 1 cut(s) 714
CviAII CATG 4 cut(s) 144, 180, 405, 592
CviJI RGCY 8 cut(s) 69, 134, 201, 250, 491, 513, 551, 686
CviKI_1 RGCY 8 cut(s) 69, 134, 201, 250, 491, 513, 551, 686
CviQI GTAC 1 cut(s) 714
DdeI CTNAG 2 cut(s) 72, 670
DpnI GATC 5 cut(s) 89, 366, 451, 557, 737
DpnII GATC 5 cut(s) 87, 364, 449, 555, 735
EaeI YGGCCR 1 cut(s) 199
Eam1104I CTCTTC 2 cut(s) 124, 385
EarI CTCTTC 2 cut(s) 124, 385
Eco130I CCWWGG 1 cut(s) 591
Eco24I GRGCYC 2 cut(s) 136, 252
Eco47I GGWCC 1 cut(s) 600
EcoNI CCTNNNNNAGG 1 cut(s) 422
EcoRI GAATTC 1 cut(s) 437
EcoRII CCWGG 1 cut(s) 385
EcoT14I CCWWGG 1 cut(s) 591
EcoT38I GRGCYC 2 cut(s) 136, 252
ErhI CCWWGG 1 cut(s) 591
FaeI CATG 4 cut(s) 147, 183, 408, 595
FaiI YATR 8 cut(s) 145, 181, 212, 214, 398, 406, 456, 593
FatI CATG 4 cut(s) 143, 179, 404, 591
FauNDI CATATG 1 cut(s) 212
Fnu4HI GCNGC 3 cut(s) 148, 288, 549
FokI GGATG 5 cut(s) 287, 392, 422, 574, 711
FriOI GRGCYC 2 cut(s) 136, 252
Fsp4HI GCNGC 3 cut(s) 148, 288, 549
FspBI CTAG 1 cut(s) 126
GluI GCNGC 3 cut(s) 148, 288, 549
GsaI CCCAGC 1 cut(s) 249
HaeIII GGCC 3 cut(s) 201, 250, 686
HapII CCGG 1 cut(s) 136
Hin1II CATG 4 cut(s) 147, 183, 408, 595
HinfI GANTC 3 cut(s) 187, 292, 675
HpaII CCGG 1 cut(s) 136
HphI GGTGA 2 cut(s) 550, 589
Hpy166II GTNNAC 2 cut(s) 159, 541
Hpy188I TCNGA 3 cut(s) 284, 423, 555
Hpy188III TCNNGA 1 cut(s) 727
Hpy8I GTNNAC 2 cut(s) 159, 541
HpyAV CCTTC 4 cut(s) 176, 418, 428, 667
HpyCH4III ACNGT 1 cut(s) 722
HpyCH4V TGCA 8 cut(s) 65, 115, 150, 216, 471, 548, 641, 647
HpyF10VI GCNNNNNNNGC 3 cut(s) 112, 207, 284
HpyF3I CTNAG 2 cut(s) 72, 670
Hsp92II CATG 4 cut(s) 147, 183, 408, 595
Kzo9I GATC 5 cut(s) 87, 364, 449, 555, 735
LguI GCTCTTC 1 cut(s) 124
LmnI GCTCC 1 cut(s) 284
Lsp1109I GCAGC 3 cut(s) 134, 299, 560
LweI GCATC 2 cut(s) 403, 596
MaeI CTAG 1 cut(s) 126
MaeIII GTNAC 1 cut(s) 326
MalI GATC 5 cut(s) 89, 366, 451, 557, 737
MboI GATC 5 cut(s) 87, 364, 449, 555, 735
MboII GAAGA 7 cut(s) 29, 141, 233, 402, 550, 670, 698
MfeI CAATTG 1 cut(s) 472
MhlI GDGCHC 5 cut(s) 78, 136, 252, 431, 584
MlsI TGGCCA 1 cut(s) 201
MluCI AATT 6 cut(s) 33, 42, 117, 253, 437, 472
MluNI TGGCCA 1 cut(s) 201
MlyI GAGTC 1 cut(s) 286
MmeI TCCRAC 2 cut(s) 466, 676
MnlI CCTC 3 cut(s) 19, 355, 572
Mox20I TGGCCA 1 cut(s) 201
MroXI GAANNNNTTC 1 cut(s) 100
MscI TGGCCA 1 cut(s) 201
MseI TTAA 2 cut(s) 32, 617
MslI CAYNNNNRTG 1 cut(s) 349
Msp20I TGGCCA 1 cut(s) 201
MspI CCGG 1 cut(s) 136
MspR9I CCNGG 2 cut(s) 136, 387
MunI CAATTG 1 cut(s) 472
Mva1269I GAATGC 1 cut(s) 623
MvaI CCWGG 1 cut(s) 387
MwoI GCNNNNNNNGC 3 cut(s) 112, 207, 284
NciI CCSGG 1 cut(s) 136
NcoI CCATGG 1 cut(s) 591
NdeI CATATG 1 cut(s) 212
NdeII GATC 5 cut(s) 87, 364, 449, 555, 735
NlaIII CATG 4 cut(s) 147, 183, 408, 595
NlaIV GGNNCC 1 cut(s) 250
NmuCI GTSAC 1 cut(s) 326
PciSI GCTCTTC 1 cut(s) 124
PctI GAATGC 1 cut(s) 623
PdmI GAANNNNTTC 1 cut(s) 100
PfeI GAWTC 2 cut(s) 187, 675
PkrI GCNGC 3 cut(s) 149, 289, 550
PleI GAGTC 1 cut(s) 286
PpsI GAGTC 1 cut(s) 286
PsiI TTATAA 1 cut(s) 456
Psp6I CCWGG 1 cut(s) 385
PspFI CCCAGC 1 cut(s) 245
PspGI CCWGG 1 cut(s) 385
PspN4I GGNNCC 1 cut(s) 250
PspOMI GGGCCC 1 cut(s) 248
PspPI GGNCC 3 cut(s) 248, 249, 600
RsaI GTAC 1 cut(s) 715
RsaNI GTAC 1 cut(s) 714
RseI CAYNNNNRTG 1 cut(s) 349
SapI GCTCTTC 1 cut(s) 124
SaqAI TTAA 2 cut(s) 32, 617
SatI GCNGC 3 cut(s) 148, 288, 549
Sau3AI GATC 5 cut(s) 87, 364, 449, 555, 735
Sau96I GGNCC 3 cut(s) 248, 249, 600
SchI GAGTC 1 cut(s) 286
ScrFI CCNGG 2 cut(s) 136, 387
SduI GDGCHC 5 cut(s) 78, 136, 252, 431, 584
SetI ASST 8 cut(s) 388, 470, 501, 509, 546, 553, 652, 659
SfaNI GCATC 2 cut(s) 403, 596
SinI GGWCC 1 cut(s) 600
SmiMI CAYNNNNRTG 1 cut(s) 349
SmlI CTYRAG 1 cut(s) 563
SmoI CTYRAG 1 cut(s) 563
Sse9I AATT 6 cut(s) 33, 42, 117, 253, 437, 472
SsiI CCGC 1 cut(s) 598
SspI AATATT 1 cut(s) 460
SspMI CTAG 1 cut(s) 126
StyD4I CCNGG 2 cut(s) 134, 385
StyI CCWWGG 1 cut(s) 591
TaaI ACNGT 1 cut(s) 722
TaqI TCGA 1 cut(s) 90
TaqII GACCGA 1 cut(s) 617
TasI AATT 6 cut(s) 33, 42, 117, 253, 437, 472
TfiI GAWTC 2 cut(s) 187, 675
Tru1I TTAA 2 cut(s) 32, 617
Tru9I TTAA 2 cut(s) 32, 617
TscAI CASTG 1 cut(s) 329
TseFI GTSAC 1 cut(s) 326
TseI GCWGC 3 cut(s) 147, 287, 548
Tsp45I GTSAC 1 cut(s) 326
TspDTI ATGAA 3 cut(s) 168, 184, 396
TspRI CASTG 1 cut(s) 329
VpaK11BI GGWCC 1 cut(s) 600
XagI CCTNNNNNAGG 1 cut(s) 422
XapI RAATTY 1 cut(s) 437
XcmI CCANNNNNNNNNTGG 1 cut(s) 177
XmnI GAANNNNTTC 1 cut(s) 100
XspI CTAG 1 cut(s) 126
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.