Rorug02G0086300

RTE1-HOMOLOG-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0005598
Physical Location & Seq
Reverse (-)
6878872 .. 6881537
2666 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0086300.1

Sequence Viewer

Length: 2013 bp
ATGCCGGTGCAACTGACGCCGAACGCGGTATCCGCCATCATCGGCGGTGATCTCAACTTGAAGCCGCTGATGCAGGTCACCGATATCAAGCTCATCGGCTCGCAGGAGAGGTACCGGCTCATGGTCTCCGACGGCGTTTGGTCTCAGCACGCCATGGTCGGCACTCAGCTCAACCAGCTGGTCAAGACCGGCCAGGTCAGGAAAGGCTCCGTCGTCCAGCTTACCGAGTACATTTGCAGCGAAGTCCAGAACCGCAAGATTATTGTTGTGCTGAATATGGAAACTATTGTATTGGACTCGGAGATTATTGGGAATCCAAAACAATATGTTGAGTCTGGTCCAACTGCTGAAACTGCATTGCCTAAGAACAATTTTCAGCAGTCTGCCTATGGCAACAATCATATGATTGGCCAAGGGCCTGTTCCCAAATGGCAGAATCCCAGGCCCTCTTTTCATCCTGAAAACTTTGTTTCCAGCATGCCAAATGCCAGACCTACATTCCAATCTGAAAGCTCTTTTTACAATACTCCGAATCCCAGACCTACATTTCGTCCTGAAAGCTCAGCTCCCAGTGTGCCAAATTTCCGAGCTACTGTGCAGCCTTCTTACCAGCCACCTCCACAGTATAAAAATCGTGGCCCCGTGTTCAAGAATGAGGCGCCAGCACGTATTATACCTATTAATGCTCTAAATCCTTATCAGGGAAGGTGGGCTATCAAGGCAAGAGTCACTGCAAAAGGGGATCAGCGTCGGTATAACAATGCTCGAGGAGATGGTAAGGTTTTCTCTTTCGACCTCCTTGACTCTGAGGGTGGGGAAATACGAGTGACCTGCTTTAATGCCGTTCTTGACCGCTTTTATGATATTATTGAGGTTGGCAAAGTTTACTTGATATCAAAGGGTAGCTTAAAACCTGCGCAGAAGAACTTTAATCATCTGAAGAGTGACTGGGAGATAACTTTGGATGCAAGTTCAACAGTGGAACTGTGCCCTGATGAAGATGGTTCTATACCAGACCAGCTGTTTGACTTCAAGCCTATTAGTGCAATTGAGAATGCTGAGAGTAATTCTATACTTGATGTTATTGGCATTGTGACATCTGTCAACCCTTCTACTACTATCCTGAGAAAGAATGGTATGGAAACTCAGAGAAGAATTCTGCATCTGAAGGATTGCTCTGGTAGGAGCGTTGAGATAACCCTATGGGGGGAGGTCTGTAACAGGGAAGGTCAAAAGCTGGAAGAGATGTTGGCTTCTGGTGTTTCTCCAGTTTTAGCTGTCAAAGCTGGGAAGGTTAATGACTTCAGTGGGAAATCCATAGGAACAATTCATTCTACCCAGCTATACATTAACCCAGATTTGCCTGAGGCTCGTCAGGTGAGGGACTGGTTTGATCGAGGGGGAAAGGATGCCGCTTCCATGTCCATTTCTAAGGAATTTATGCCAGGAGGAGTAACTAAGAATGACATACGCAAAACTGTTTCTCAGATCAAGAATGAAGGTCTTGGAAGATCAGATAAGCCAGACTGGGTCACTGTGAGGGCCACCATATCTTTCATTAAAACCGATTCTTTCTGTTACACAGCATGCCCGTTGATGATTGGAGATAGACAGTGTAGCAAGAAAGTGTCGAGGTCAGGAGTTGGAGGATGGCAGTGTGACAGGTGCAATCAAGAATTTGAGGAGTGTGATTATAGATATCTTCTTCAAGCCCAAATTCAGGACCATACTGGATTGACTTGGGTGACGGCTTTTCAGGAATCAGGGGCAGAGATCTTGGGTTGCACAGCAAAGGAGTTGTACTTTTTGAAGTACGAGGGGCAGGATGAGGAAAAGTTCGCAGACATAGTCCGGAGCAGTATTTTCAACCAGTTTATTTTCAAGCTTAAGATCAAAGAGGAGTTGTATGGCGACGAGCAGAGGGTGAAGATCACAGTGGTTAAGGCAGATACGGTGAACTATTCTGCAGAGAGCAGATACATGCTTGATGTGATTTCGAAGCTTTCTAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04016 map04016
Pfam Domains
Protein Families
DUF778

Protein Analysis

670

Amino Acids

74.95

Weight (kDa)

8.1

Isoelectric Point (pI)

35.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rep-A_N PF04057 5 - 97 1.8e-28 Replication factor-A protein 1, N-terminal domain
tRNA_anti-codon PF01336 239 - 319 2.3e-08 OB-fold nucleic acid binding domain
REPA_OB_2 PF16900 347 - 451 3.7e-28 Replication protein A OB domain
Rep_fac-A_C PF08646 510 - 661 1.1e-50 Replication factor-A C terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 918
Acc36I ACCTGC 3 cut(s) 64, 839, 922
Acc65I GGTACC 1 cut(s) 111
AccB1I GGYRCC 2 cut(s) 111, 658
AccII CGCG 1 cut(s) 26
AccIII TCCGGA 1 cut(s) 1851
AciI CCGC 7 cut(s) 26, 33, 45, 65, 253, 853, 1413
AclWI GGATC 1 cut(s) 750
AcoI YGGCCR 2 cut(s) 190, 409
AcsI RAATTY 5 cut(s) 580, 1155, 1436, 1676, 1716
AcuI CTGAAG 3 cut(s) 959, 1187, 1288
AcyI GRCGYC 2 cut(s) 17, 659
AfaI GTAC 4 cut(s) 113, 230, 1802, 1814
AfiI CCNNNNNNNGG 5 cut(s) 121, 701, 1206, 1207, 1720
AflII CTTAAG 1 cut(s) 1886
AgsI TTSAA 8 cut(s) 61, 649, 975, 1033, 1709, 1810, 1867, 1882
AjnI CCWGG 3 cut(s) 192, 440, 1444
AjuI GAANNNNNNNTTGG 2 cut(s) 405, 437
AloI GAACNNNNNNTCC 2 cut(s) 14, 46
Alw26I GTCTC 2 cut(s) 130, 147
AlwI GGATC 1 cut(s) 750
Ama87I CYCGRG 1 cut(s) 765
Aor13HI TCCGGA 1 cut(s) 1851
AoxI GGCC 6 cut(s) 190, 409, 416, 443, 637, 1542
ApeKI GCWGC 2 cut(s) 237, 598
ApoI RAATTY 5 cut(s) 580, 1155, 1436, 1676, 1716
ArsI GACNNNNNNTTYG 4 cut(s) 531, 563, 1007, 1039
AseI ATTAAT 1 cut(s) 681
Asp718I GGTACC 1 cut(s) 111
AspLEI GCGC 2 cut(s) 661, 919
AspS9I GGNCC 6 cut(s) 338, 416, 444, 638, 1542, 1723
AsuHPI GGTGA 6 cut(s) 59, 70, 1390, 1756, 1936, 1966
AsuII TTCGAA 1 cut(s) 1997
AvaI CYCGRG 1 cut(s) 765
AvaII GGWCC 2 cut(s) 338, 1723
AxyI CCTNAGG 1 cut(s) 1365
BaeGI GKGCMC 1 cut(s) 992
BalI TGGCCA 1 cut(s) 411
BanI GGYRCC 2 cut(s) 111, 658
BbvI GCAGC 2 cut(s) 249, 610
BccI CCATC 4 cut(s) 44, 767, 995, 1644
BceAI ACGGC 3 cut(s) 148, 827, 1764
BcgI CGANNNNNNTGC 2 cut(s) 813, 847
BciT130I CCWGG 3 cut(s) 194, 442, 1446
BciVI GTATCC 1 cut(s) 40
BcoDI GTCTC 2 cut(s) 130, 147
BfaI CTAG 1 cut(s) 2007
BfmI CTRYAG 1 cut(s) 1965
BfoI RGCGCY 1 cut(s) 662
BfrI CTTAAG 1 cut(s) 1886
BfuAI ACCTGC 3 cut(s) 64, 839, 922
BfuI GTATCC 1 cut(s) 40
BglII AGATCT 1 cut(s) 1773
BisI GCNGC 4 cut(s) 65, 238, 599, 1413
BlpI GCTNAGC 1 cut(s) 562
BlsI GCNGC 4 cut(s) 66, 239, 600, 1414
Bme1390I CCNGG 3 cut(s) 194, 442, 1446
Bme18I GGWCC 2 cut(s) 338, 1723
BmeT110I CYCGRG 1 cut(s) 765
BmgT120I GGNCC 6 cut(s) 338, 416, 444, 638, 1542, 1723
BmiI GGNNCC 4 cut(s) 113, 208, 640, 660
BmrFI CCNGG 3 cut(s) 194, 442, 1446
BmrI ACTGGG 3 cut(s) 564, 958, 1537
BmsI GCATC 4 cut(s) 60, 955, 1171, 1399
BmuI ACTGGG 3 cut(s) 564, 958, 1537
BoxI GACNNNNGTC 1 cut(s) 1100
BpmI CTGGAG 1 cut(s) 1251
Bpu1102I GCTNAGC 1 cut(s) 562
Bpu14I TTCGAA 1 cut(s) 1997
BsaAI YACGTR 1 cut(s) 668
BsaHI GRCGYC 2 cut(s) 17, 659
BsaI GGTCTC 2 cut(s) 130, 147
BsaJI CCNNGG 3 cut(s) 153, 412, 440
BsaWI WCCGGW 1 cut(s) 1851
Bsc4I CCNNNNNNNGG 5 cut(s) 121, 701, 1206, 1207, 1720
Bse118I RCCGGY 3 cut(s) 4, 114, 188
Bse1I ACTGG 7 cut(s) 570, 953, 1268, 1391, 1532, 1735, 1870
Bse21I CCTNAGG 1 cut(s) 1365
Bse3DI GCAATG 1 cut(s) 356
BseAI TCCGGA 1 cut(s) 1851
BseBI CCWGG 3 cut(s) 194, 442, 1446
BseDI CCNNGG 3 cut(s) 153, 412, 440
BseGI GGATG 5 cut(s) 454, 970, 1414, 1655, 1831
BseLI CCNNNNNNNGG 5 cut(s) 121, 701, 1206, 1207, 1720
BseMI GCAATG 1 cut(s) 356
BseMII CTCAG 9 cut(s) 158, 179, 576, 798, 1050, 1115, 1160, 1356, 1499
BseNI ACTGG 7 cut(s) 570, 953, 1268, 1391, 1532, 1735, 1870
BseRI GAGGAG 4 cut(s) 783, 1464, 1697, 1913
BseSI GKGCMC 1 cut(s) 992
BseXI GCAGC 2 cut(s) 249, 610
BseYI CCCAGC 2 cut(s) 1286, 1338
BsgI GTGCAG 1 cut(s) 617
Bsh1236I CGCG 1 cut(s) 26
BshFI GGCC 6 cut(s) 192, 411, 418, 445, 639, 1544
BshNI GGYRCC 2 cut(s) 111, 658
BsiHKCI CYCGRG 1 cut(s) 765
BsiSI CCGG 4 cut(s) 5, 115, 189, 1852
BslFI GGGAC 1 cut(s) 1397
BslI CCNNNNNNNGG 5 cut(s) 121, 701, 1206, 1207, 1720
BsmAI GTCTC 2 cut(s) 130, 147
BsmFI GGGAC 1 cut(s) 1397
BsmI GAATGC 1 cut(s) 1060
BsnI GGCC 6 cut(s) 192, 411, 418, 445, 639, 1544
Bso31I GGTCTC 2 cut(s) 130, 147
BsoBI CYCGRG 1 cut(s) 765
Bsp119I TTCGAA 1 cut(s) 1997
Bsp1286I GDGCHC 1 cut(s) 992
Bsp13I TCCGGA 1 cut(s) 1851
Bsp143I GATC 8 cut(s) 49, 742, 1393, 1488, 1511, 1773, 1890, 1929
Bsp1720I GCTNAGC 1 cut(s) 562
Bsp19I CCATGG 1 cut(s) 153
BspACI CCGC 7 cut(s) 26, 33, 45, 65, 253, 853, 1413
BspANI GGCC 6 cut(s) 192, 411, 418, 445, 639, 1544
BspCNI CTCAG 9 cut(s) 157, 178, 575, 799, 1051, 1116, 1159, 1357, 1498
BspEI TCCGGA 1 cut(s) 1851
BspFNI CGCG 1 cut(s) 26
BspLI GGNNCC 4 cut(s) 113, 208, 640, 660
BspMAI CTGCAG 1 cut(s) 1969
BspMI ACCTGC 3 cut(s) 64, 839, 922
BspPI GGATC 1 cut(s) 750
BspT104I TTCGAA 1 cut(s) 1997
BspT107I GGYRCC 2 cut(s) 111, 658
BspTI CTTAAG 1 cut(s) 1886
BspTNI GGTCTC 2 cut(s) 130, 147
BsrDI GCAATG 1 cut(s) 356
BsrFI RCCGGY 3 cut(s) 4, 114, 188
BsrI ACTGG 7 cut(s) 570, 953, 1268, 1391, 1532, 1735, 1870
BssAI RCCGGY 3 cut(s) 4, 114, 188
BssECI CCNNGG 3 cut(s) 153, 412, 440
BssMI GATC 8 cut(s) 49, 742, 1393, 1488, 1511, 1773, 1890, 1929
BssNI GRCGYC 2 cut(s) 17, 659
BssT1I CCWWGG 2 cut(s) 153, 412
Bst2UI CCWGG 3 cut(s) 194, 442, 1446
Bst4CI ACNGT 9 cut(s) 595, 624, 979, 987, 1480, 1537, 1614, 1936, 1954
Bst6I CTCTTC 2 cut(s) 935, 1236
BstACI GRCGYC 2 cut(s) 17, 659
BstAFI CTTAAG 1 cut(s) 1886
BstBAI YACGTR 1 cut(s) 668
BstBI TTCGAA 1 cut(s) 1997
BstC8I GCNNGC 5 cut(s) 101, 150, 479, 663, 1588
BstDSI CCRYGG 1 cut(s) 153
BstEII GGTNACC 1 cut(s) 76
BstF5I GGATG 5 cut(s) 454, 970, 1414, 1655, 1831
BstFNI CGCG 1 cut(s) 26
BstH2I RGCGCY 1 cut(s) 662
BstHHI GCGC 2 cut(s) 661, 919
BstKTI GATC 8 cut(s) 52, 745, 1396, 1491, 1514, 1776, 1893, 1932
BstMAI GTCTC 2 cut(s) 130, 147
BstMBI GATC 8 cut(s) 49, 742, 1393, 1488, 1511, 1773, 1890, 1929
BstMWI GCNNNNNNNGC 7 cut(s) 16, 32, 70, 175, 353, 719, 1283
BstNI CCWGG 3 cut(s) 194, 442, 1446
BstNSI RCATGY 3 cut(s) 481, 1590, 1984
BstPAI GACNNNNGTC 1 cut(s) 1100
BstPI GGTNACC 1 cut(s) 76
BstSCI CCNGG 3 cut(s) 192, 440, 1444
BstSFI CTRYAG 1 cut(s) 1965
BstSLI GKGCMC 1 cut(s) 992
BstUI CGCG 1 cut(s) 26
BstV1I GCAGC 2 cut(s) 249, 610
BstX2I RGATCY 1 cut(s) 1773
BstYI RGATCY 1 cut(s) 1773
Bsu36I CCTNAGG 1 cut(s) 1365
BsuI GTATCC 1 cut(s) 40
BsuRI GGCC 6 cut(s) 192, 411, 418, 445, 639, 1544
BtgI CCRYGG 1 cut(s) 153
BtsCI GGATG 5 cut(s) 454, 970, 1414, 1655, 1831
BtsI GCAGTG 2 cut(s) 729, 1661
BtsIMutI CAGTG 8 cut(s) 577, 729, 984, 1312, 1533, 1619, 1661, 1941
BveI ACCTGC 3 cut(s) 64, 839, 922
Cac8I GCNNGC 5 cut(s) 101, 150, 479, 663, 1588
CfoI GCGC 2 cut(s) 661, 919
Cfr10I RCCGGY 3 cut(s) 4, 114, 188
Cfr13I GGNCC 6 cut(s) 338, 416, 444, 638, 1542, 1723
CseI GACGC 2 cut(s) 25, 737
Csp6I GTAC 4 cut(s) 112, 229, 1801, 1813
CviAII CATG 6 cut(s) 121, 154, 478, 1420, 1587, 1981
CviQI GTAC 4 cut(s) 112, 229, 1801, 1813
DinI GGCGCC 1 cut(s) 660
DpnI GATC 8 cut(s) 51, 744, 1395, 1490, 1513, 1775, 1892, 1931
DpnII GATC 8 cut(s) 49, 742, 1393, 1488, 1511, 1773, 1890, 1929
EaeI YGGCCR 2 cut(s) 190, 409
Eam1104I CTCTTC 2 cut(s) 935, 1236
EarI CTCTTC 2 cut(s) 935, 1236
EciI GGCGGA 1 cut(s) 22
Eco130I CCWWGG 2 cut(s) 153, 412
Eco31I GGTCTC 2 cut(s) 130, 147
Eco32I GATATC 3 cut(s) 85, 894, 1700
Eco47I GGWCC 2 cut(s) 338, 1723
Eco57I CTGAAG 3 cut(s) 959, 1187, 1288
Eco81I CCTNAGG 1 cut(s) 1365
Eco88I CYCGRG 1 cut(s) 765
Eco91I GGTNACC 1 cut(s) 76
EcoO109I RGGNCCY 2 cut(s) 416, 444
EcoO65I GGTNACC 1 cut(s) 76
EcoRI GAATTC 1 cut(s) 1155
EcoRII CCWGG 3 cut(s) 192, 440, 1444
EcoRV GATATC 3 cut(s) 85, 894, 1700
EcoT14I CCWWGG 2 cut(s) 153, 412
EgeI GGCGCC 1 cut(s) 660
EheI GGCGCC 1 cut(s) 660
ErhI CCWWGG 2 cut(s) 153, 412
FaeI CATG 6 cut(s) 124, 157, 481, 1423, 1590, 1984
FalI AAGNNNNNCTT 2 cut(s) 890, 922
FaqI GGGAC 1 cut(s) 1397
FatI CATG 6 cut(s) 120, 153, 477, 1419, 1586, 1980
FauNDI CATATG 1 cut(s) 402
Fnu4HI GCNGC 4 cut(s) 65, 238, 599, 1413
FokI GGATG 5 cut(s) 441, 977, 1421, 1662, 1838
Fsp4HI GCNGC 4 cut(s) 65, 238, 599, 1413
FspBI CTAG 1 cut(s) 2007
FspI TGCGCA 1 cut(s) 918
GlaI GCGC 2 cut(s) 660, 918
GluI GCNGC 4 cut(s) 65, 238, 599, 1413
GsaI CCCAGC 2 cut(s) 1290, 1342
GsuI CTGGAG 1 cut(s) 1251
HaeII RGCGCY 1 cut(s) 662
HaeIII GGCC 6 cut(s) 192, 411, 418, 445, 639, 1544
HapII CCGG 4 cut(s) 5, 115, 189, 1852
HgaI GACGC 2 cut(s) 25, 737
HhaI GCGC 2 cut(s) 661, 919
Hin1I GRCGYC 2 cut(s) 17, 659
Hin1II CATG 6 cut(s) 124, 157, 481, 1423, 1590, 1984
Hin6I GCGC 2 cut(s) 659, 917
HinP1I GCGC 2 cut(s) 659, 917
HincII GTYRAC 1 cut(s) 1105
HindII GTYRAC 1 cut(s) 1105
HindIII AAGCTT 2 cut(s) 1883, 2000
HinfI GANTC 9 cut(s) 296, 313, 332, 436, 532, 726, 803, 1568, 1760
HpaII CCGG 4 cut(s) 5, 115, 189, 1852
HphI GGTGA 6 cut(s) 59, 70, 1390, 1756, 1936, 1966
Hpy166II GTNNAC 3 cut(s) 886, 1105, 1957
Hpy8I GTNNAC 3 cut(s) 886, 1105, 1957
Hpy99I CGWCG 4 cut(s) 134, 215, 753, 1916
HpyAV CCTTC 7 cut(s) 612, 699, 1119, 1162, 1220, 1285, 1493
HpyCH4III ACNGT 9 cut(s) 595, 624, 979, 987, 1480, 1537, 1614, 1936, 1954
HpyCH4IV ACGT 1 cut(s) 667
HpyF10VI GCNNNNNNNGC 7 cut(s) 16, 32, 70, 175, 353, 719, 1283
HpySE526I ACGT 1 cut(s) 667
Hsp92I GRCGYC 2 cut(s) 17, 659
Hsp92II CATG 6 cut(s) 124, 157, 481, 1423, 1590, 1984
HspAI GCGC 2 cut(s) 659, 917
KasI GGCGCC 1 cut(s) 658
Kpn2I TCCGGA 1 cut(s) 1851
KpnI GGTACC 1 cut(s) 115
Kzo9I GATC 8 cut(s) 49, 742, 1393, 1488, 1511, 1773, 1890, 1929
LmnI GCTCC 4 cut(s) 212, 571, 1185, 1854
Lsp1109I GCAGC 2 cut(s) 249, 610
LweI GCATC 4 cut(s) 60, 955, 1171, 1399
MaeI CTAG 1 cut(s) 2007
MaeII ACGT 1 cut(s) 667
MalI GATC 8 cut(s) 51, 744, 1395, 1490, 1513, 1775, 1892, 1931
MboI GATC 8 cut(s) 49, 742, 1393, 1488, 1511, 1773, 1890, 1929
MboII GAAGA 9 cut(s) 934, 952, 1010, 1164, 1253, 1521, 1694, 1697, 1939
MfeI CAATTG 1 cut(s) 1047
MflI RGATCY 1 cut(s) 1773
MhlI GDGCHC 1 cut(s) 992
MlsI TGGCCA 1 cut(s) 411
MluCI AATT 9 cut(s) 370, 580, 1047, 1066, 1155, 1326, 1436, 1676, 1716
MluNI TGGCCA 1 cut(s) 411
Mly113I GGCGCC 1 cut(s) 659
MlyI GAGTC 4 cut(s) 290, 341, 735, 797
MmeI TCCRAC 3 cut(s) 153, 365, 1624
Mox20I TGGCCA 1 cut(s) 411
MroI TCCGGA 1 cut(s) 1851
MscI TGGCCA 1 cut(s) 411
MseI TTAA 9 cut(s) 681, 837, 908, 930, 1296, 1350, 1560, 1887, 1941
Msp20I TGGCCA 1 cut(s) 411
MspA1I CMGCKG 3 cut(s) 67, 178, 1021
MspCI CTTAAG 1 cut(s) 1886
MspI CCGG 4 cut(s) 5, 115, 189, 1852
MspR9I CCNGG 3 cut(s) 194, 442, 1446
MunI CAATTG 1 cut(s) 1047
Mva1269I GAATGC 1 cut(s) 1060
MvaI CCWGG 3 cut(s) 194, 442, 1446
MvnI CGCG 1 cut(s) 26
MwoI GCNNNNNNNGC 7 cut(s) 16, 32, 70, 175, 353, 719, 1283
NarI GGCGCC 1 cut(s) 659
NcoI CCATGG 1 cut(s) 153
NdeI CATATG 1 cut(s) 402
NdeII GATC 8 cut(s) 49, 742, 1393, 1488, 1511, 1773, 1890, 1929
NlaIII CATG 6 cut(s) 124, 157, 481, 1423, 1590, 1984
NlaIV GGNNCC 4 cut(s) 113, 208, 640, 660
NmuCI GTSAC 8 cut(s) 76, 727, 826, 944, 1093, 1531, 1658, 1744
NsbI TGCGCA 1 cut(s) 918
NspI RCATGY 3 cut(s) 481, 1590, 1984
NspV TTCGAA 1 cut(s) 1997
PaeI GCATGC 2 cut(s) 481, 1590
PaeR7I CTCGAG 1 cut(s) 765
PctI GAATGC 1 cut(s) 1060
PfeI GAWTC 5 cut(s) 313, 436, 532, 1568, 1760
PflFI GACNNNGTC 2 cut(s) 1529, 1847
PkrI GCNGC 4 cut(s) 66, 239, 600, 1414
PleI GAGTC 4 cut(s) 290, 340, 734, 797
PluTI GGCGCC 1 cut(s) 662
PpsI GAGTC 4 cut(s) 290, 340, 734, 797
Ppu21I YACGTR 1 cut(s) 668
PshAI GACNNNNGTC 1 cut(s) 1100
PshBI ATTAAT 1 cut(s) 681
Psp6I CCWGG 3 cut(s) 192, 440, 1444
PspEI GGTNACC 1 cut(s) 76
PspFI CCCAGC 2 cut(s) 1286, 1338
PspGI CCWGG 3 cut(s) 192, 440, 1444
PspN4I GGNNCC 4 cut(s) 113, 208, 640, 660
PspPI GGNCC 6 cut(s) 338, 416, 444, 638, 1542, 1723
PspXI VCTCGAGB 1 cut(s) 765
PstI CTGCAG 1 cut(s) 1969
PsuI RGATCY 1 cut(s) 1773
PsyI GACNNNGTC 2 cut(s) 1529, 1847
PvuII CAGCTG 2 cut(s) 178, 1021
RsaI GTAC 4 cut(s) 113, 230, 1802, 1814
RsaNI GTAC 4 cut(s) 112, 229, 1801, 1813
SaqAI TTAA 9 cut(s) 681, 837, 908, 930, 1296, 1350, 1560, 1887, 1941
SatI GCNGC 4 cut(s) 65, 238, 599, 1413
Sau3AI GATC 8 cut(s) 49, 742, 1393, 1488, 1511, 1773, 1890, 1929
Sau96I GGNCC 6 cut(s) 338, 416, 444, 638, 1542, 1723
SchI GAGTC 4 cut(s) 290, 341, 735, 797
ScrFI CCNGG 3 cut(s) 194, 442, 1446
SduI GDGCHC 1 cut(s) 992
SfaNI GCATC 4 cut(s) 60, 955, 1171, 1399
SfcI CTRYAG 1 cut(s) 1965
SfoI GGCGCC 1 cut(s) 660
Sfr274I CTCGAG 1 cut(s) 765
SfuI TTCGAA 1 cut(s) 1997
SinI GGWCC 2 cut(s) 338, 1723
SlaI CTCGAG 1 cut(s) 765
SmlI CTYRAG 2 cut(s) 765, 1886
SmoI CTYRAG 2 cut(s) 765, 1886
SphI GCATGC 2 cut(s) 481, 1590
Sse9I AATT 9 cut(s) 370, 580, 1047, 1066, 1155, 1326, 1436, 1676, 1716
SsiI CCGC 7 cut(s) 26, 33, 45, 65, 253, 853, 1413
SspDI GGCGCC 1 cut(s) 658
SspMI CTAG 1 cut(s) 2007
StyD4I CCNGG 3 cut(s) 192, 440, 1444
StyI CCWWGG 2 cut(s) 153, 412
TaaI ACNGT 9 cut(s) 595, 624, 979, 987, 1480, 1537, 1614, 1936, 1954
TaiI ACGT 1 cut(s) 670
TaqI TCGA 5 cut(s) 766, 792, 1396, 1631, 1997
TasI AATT 9 cut(s) 370, 580, 1047, 1066, 1155, 1326, 1436, 1676, 1716
TatI WGTACW 2 cut(s) 228, 1800
TauI GCSGC 2 cut(s) 67, 1415
TfiI GAWTC 5 cut(s) 313, 436, 532, 1568, 1760
Tru1I TTAA 9 cut(s) 681, 837, 908, 930, 1296, 1350, 1560, 1887, 1941
Tru9I TTAA 9 cut(s) 681, 837, 908, 930, 1296, 1350, 1560, 1887, 1941
TscAI CASTG 8 cut(s) 577, 736, 984, 1312, 1540, 1619, 1661, 1941
TseFI GTSAC 8 cut(s) 76, 727, 826, 944, 1093, 1531, 1658, 1744
TseI GCWGC 2 cut(s) 237, 598
Tsp45I GTSAC 8 cut(s) 76, 727, 826, 944, 1093, 1531, 1658, 1744
TspDTI ATGAA 5 cut(s) 443, 1011, 1319, 1512, 1546
TspGWI ACGGA 1 cut(s) 199
TspRI CASTG 8 cut(s) 577, 736, 984, 1312, 1540, 1619, 1661, 1941
Tth111I GACNNNGTC 2 cut(s) 1529, 1847
Vha464I CTTAAG 1 cut(s) 1886
VpaK11BI GGWCC 2 cut(s) 338, 1723
VspI ATTAAT 1 cut(s) 681
XapI RAATTY 5 cut(s) 580, 1155, 1436, 1676, 1716
XceI RCATGY 3 cut(s) 481, 1590, 1984
XhoI CTCGAG 1 cut(s) 765
XspI CTAG 1 cut(s) 2007
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.