MD02G1137300.v1.1

RTE1-HOMOLOG-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0005598
Physical Location & Seq
Forward (+)
11476322 .. 11478492
2171 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1137300.v1.1.491

Sequence Viewer

Length: 717 bp
ATGCAGCAGCTTCCAATTTTGGCAATGGAAACAAGTGCAGGCCCCGAGCACAACTTGAGGATTGATGGAAGTGATTCACCGACTTTGCAAATTGACCCCAAAAGATCCCGGTTCCCATGCTGCATTGTGTGGACACCCCTTCCTGTTATTTCATGGTTGGTTCCTTTCGTTGGCCACATTGGCATATGCAGAGAAGATGGCGTGATATTGGACTTTGCAGGGCCCAATTTCGTCTGTGTGGATAACTTTTCTTTTGGAGCAGCAACTCGCTACATCCAAATAAACAAAGAAAAGTGTTGCGCTATTACCGATCTGTCTGAGTACCAAAGTGAGGATCAATATGGGGAGAATGAACCCGGTAGAGACATAAAGACATGGGATGATGCACTCCAGAAGGGCACTCAGGAATTCCAACACCGAGCTTACAACCTTTTTACCTGCAATTGCCACTCCTTTGTAGCCAACAACCTAAACAGGTTAGGGTTTCGGTCTGGTGGGTGGAATGTGGTGAACCTTGCAGCTCTGATCTTCCTCAAGGGTCAATGGGTGAGCACAGCAGCCGTGGTGCAATCCTTACTACCGTTTTTAATAATATTCTGTCTCGGAGTTGCATTAGCAGGTTCAACCTTCCTAACGTACCTGGGTTTCTTCACCGCCTTTCTCGTCGGTTGGTTTCTTCTTGGTACGTACTGTTTCAAGAGTTTGATCCAATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04016 map04016
Pfam Domains
Protein Families
DUF778

Protein Analysis

239

Amino Acids

26.57

Weight (kDa)

5.52

Isoelectric Point (pI)

37.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RTE1 PF05608 32 - 180 2.9e-71 RTE1-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 446, 608
AciI CCGC 1 cut(s) 654
AclWI GGATC 3 cut(s) 99, 342, 700
AcoI YGGCCR 1 cut(s) 172
AcsI RAATTY 1 cut(s) 407
AfaI GTAC 4 cut(s) 323, 638, 685, 689
AfiI CCNNNNNNNGG 2 cut(s) 170, 331
AgsI TTSAA 2 cut(s) 624, 697
AjnI CCWGG 1 cut(s) 639
AluBI AGCT 3 cut(s) 10, 422, 521
AluI AGCT 3 cut(s) 10, 422, 521
Alw21I GWGCWC 2 cut(s) 51, 554
Alw26I GTCTC 2 cut(s) 357, 605
AlwI GGATC 3 cut(s) 99, 342, 700
Ama87I CYCGRG 1 cut(s) 44
AoxI GGCC 3 cut(s) 40, 172, 221
ApaI GGGCCC 1 cut(s) 225
ApeKI GCWGC 6 cut(s) 4, 7, 120, 260, 518, 557
ApoI RAATTY 1 cut(s) 407
Asp700I GAANNNNTTC 1 cut(s) 73
AspLEI GCGC 1 cut(s) 302
AspS9I GGNCC 3 cut(s) 41, 221, 222
AsuC2I CCSGG 2 cut(s) 109, 357
AsuHPI GGTGA 4 cut(s) 69, 520, 559, 643
AvaI CYCGRG 1 cut(s) 44
BaeGI GKGCMC 2 cut(s) 225, 401
BalI TGGCCA 1 cut(s) 174
BanII GRGCYC 1 cut(s) 225
Bbv12I GWGCWC 2 cut(s) 51, 554
BbvI GCAGC 6 cut(s) 16, 19, 107, 272, 530, 569
BccI CCATC 2 cut(s) 59, 191
BceAI ACGGC 1 cut(s) 545
BciT130I CCWGG 1 cut(s) 641
BcnI CCSGG 2 cut(s) 109, 357
BcoDI GTCTC 2 cut(s) 357, 605
BfuAI ACCTGC 2 cut(s) 446, 608
BglI GCCNNNNNGGC 1 cut(s) 180
BisI GCNGC 6 cut(s) 5, 8, 121, 261, 519, 558
BlsI GCNGC 6 cut(s) 6, 9, 122, 262, 520, 559
Bme1390I CCNGG 3 cut(s) 109, 357, 641
BmeT110I CYCGRG 1 cut(s) 44
BmgT120I GGNCC 3 cut(s) 41, 221, 222
BmiI GGNNCC 4 cut(s) 43, 113, 162, 223
BmrFI CCNGG 3 cut(s) 109, 357, 641
BmsI GCATC 1 cut(s) 373
BpmI CTGGAG 1 cut(s) 374
BpuEI CTTGAG 2 cut(s) 76, 518
BpuMI CCSGG 2 cut(s) 109, 357
BsaAI YACGTR 1 cut(s) 687
BsaJI CCNNGG 2 cut(s) 561, 640
Bsc4I CCNNNNNNNGG 2 cut(s) 170, 331
Bse3DI GCAATG 1 cut(s) 30
BseBI CCWGG 1 cut(s) 641
BseDI CCNNGG 2 cut(s) 561, 640
BseGI GGATG 2 cut(s) 273, 385
BseLI CCNNNNNNNGG 2 cut(s) 170, 331
BseMI GCAATG 1 cut(s) 30
BseMII CTCAG 2 cut(s) 309, 416
BseSI GKGCMC 2 cut(s) 225, 401
BseXI GCAGC 6 cut(s) 16, 19, 107, 272, 530, 569
BsgI GTGCAG 1 cut(s) 57
BshFI GGCC 3 cut(s) 42, 174, 223
BsiHKAI GWGCWC 2 cut(s) 51, 554
BsiHKCI CYCGRG 1 cut(s) 44
BsiSI CCGG 2 cut(s) 109, 357
BslI CCNNNNNNNGG 2 cut(s) 170, 331
BsmAI GTCTC 2 cut(s) 357, 605
BsnI GGCC 3 cut(s) 42, 174, 223
BsoBI CYCGRG 1 cut(s) 44
Bsp120I GGGCCC 1 cut(s) 221
Bsp1286I GDGCHC 4 cut(s) 51, 225, 401, 554
Bsp143I GATC 5 cut(s) 104, 310, 334, 525, 705
BspACI CCGC 1 cut(s) 654
BspANI GGCC 3 cut(s) 42, 174, 223
BspCNI CTCAG 2 cut(s) 310, 415
BspLI GGNNCC 4 cut(s) 43, 113, 162, 223
BspMI ACCTGC 2 cut(s) 446, 608
BspPI GGATC 3 cut(s) 99, 342, 700
BsrDI GCAATG 1 cut(s) 30
BssECI CCNNGG 2 cut(s) 561, 640
BssMI GATC 5 cut(s) 104, 310, 334, 525, 705
Bst2UI CCWGG 1 cut(s) 641
Bst4CI ACNGT 2 cut(s) 582, 692
BstBAI YACGTR 1 cut(s) 687
BstC8I GCNNGC 1 cut(s) 40
BstDEI CTNAG 2 cut(s) 318, 402
BstDSI CCRYGG 1 cut(s) 561
BstF5I GGATG 2 cut(s) 273, 385
BstHHI GCGC 1 cut(s) 302
BstKTI GATC 5 cut(s) 107, 313, 337, 528, 708
BstMAI GTCTC 2 cut(s) 357, 605
BstMBI GATC 5 cut(s) 104, 310, 334, 525, 705
BstMWI GCNNNNNNNGC 1 cut(s) 180
BstNI CCWGG 1 cut(s) 641
BstSCI CCNGG 3 cut(s) 107, 355, 639
BstSLI GKGCMC 2 cut(s) 225, 401
BstSNI TACGTA 1 cut(s) 687
BstV1I GCAGC 6 cut(s) 16, 19, 107, 272, 530, 569
BstX2I RGATCY 1 cut(s) 104
BstYI RGATCY 1 cut(s) 104
BsuRI GGCC 3 cut(s) 42, 174, 223
BtgI CCRYGG 1 cut(s) 561
BtsCI GGATG 2 cut(s) 273, 385
BveI ACCTGC 2 cut(s) 446, 608
Cac8I GCNNGC 1 cut(s) 40
CfoI GCGC 1 cut(s) 302
Cfr13I GGNCC 3 cut(s) 41, 221, 222
Csp6I GTAC 4 cut(s) 322, 637, 684, 688
CviAII CATG 3 cut(s) 117, 153, 375
CviJI RGCY 8 cut(s) 10, 42, 174, 223, 422, 461, 521, 560
CviKI_1 RGCY 8 cut(s) 10, 42, 174, 223, 422, 461, 521, 560
CviQI GTAC 4 cut(s) 322, 637, 684, 688
DdeI CTNAG 2 cut(s) 318, 402
DpnI GATC 5 cut(s) 106, 312, 336, 527, 707
DpnII GATC 5 cut(s) 104, 310, 334, 525, 705
EaeI YGGCCR 1 cut(s) 172
Eco105I TACGTA 1 cut(s) 687
Eco24I GRGCYC 1 cut(s) 225
Eco88I CYCGRG 1 cut(s) 44
EcoO109I RGGNCCY 2 cut(s) 41, 221
EcoRI GAATTC 1 cut(s) 407
EcoRII CCWGG 1 cut(s) 639
EcoT38I GRGCYC 1 cut(s) 225
FaeI CATG 3 cut(s) 120, 156, 378
FaiI YATR 7 cut(s) 118, 154, 185, 187, 342, 368, 376
FatI CATG 3 cut(s) 116, 152, 374
FauNDI CATATG 1 cut(s) 185
Fnu4HI GCNGC 6 cut(s) 5, 8, 121, 261, 519, 558
FokI GGATG 2 cut(s) 260, 392
FriOI GRGCYC 1 cut(s) 225
Fsp4HI GCNGC 6 cut(s) 5, 8, 121, 261, 519, 558
GlaI GCGC 1 cut(s) 301
GluI GCNGC 6 cut(s) 5, 8, 121, 261, 519, 558
GsuI CTGGAG 1 cut(s) 374
HaeIII GGCC 3 cut(s) 42, 174, 223
HapII CCGG 2 cut(s) 109, 357
HhaI GCGC 1 cut(s) 302
Hin1II CATG 3 cut(s) 120, 156, 378
Hin6I GCGC 1 cut(s) 300
HinP1I GCGC 1 cut(s) 300
HinfI GANTC 1 cut(s) 74
HpaII CCGG 2 cut(s) 109, 357
HphI GGTGA 4 cut(s) 69, 520, 559, 643
Hpy166II GTNNAC 2 cut(s) 132, 511
Hpy188I TCNGA 3 cut(s) 319, 525, 605
Hpy188III TCNNGA 3 cut(s) 391, 404, 697
Hpy8I GTNNAC 2 cut(s) 132, 511
Hpy99I CGWCG 1 cut(s) 668
HpyAV CCTTC 3 cut(s) 149, 388, 637
HpyCH4III ACNGT 2 cut(s) 582, 692
HpyCH4IV ACGT 2 cut(s) 635, 686
HpyF10VI GCNNNNNNNGC 1 cut(s) 180
HpyF3I CTNAG 2 cut(s) 318, 402
HpySE526I ACGT 2 cut(s) 635, 686
Hsp92II CATG 3 cut(s) 120, 156, 378
HspAI GCGC 1 cut(s) 300
Kzo9I GATC 5 cut(s) 104, 310, 334, 525, 705
LmnI GCTCC 1 cut(s) 257
Lsp1109I GCAGC 6 cut(s) 16, 19, 107, 272, 530, 569
LweI GCATC 1 cut(s) 373
MaeII ACGT 2 cut(s) 635, 686
MalI GATC 5 cut(s) 106, 312, 336, 527, 707
MboI GATC 5 cut(s) 104, 310, 334, 525, 705
MboII GAAGA 4 cut(s) 206, 520, 640, 668
MfeI CAATTG 2 cut(s) 442, 710
MflI RGATCY 1 cut(s) 104
MhlI GDGCHC 4 cut(s) 51, 225, 401, 554
MlsI TGGCCA 1 cut(s) 174
MluCI AATT 6 cut(s) 15, 90, 226, 407, 442, 710
MluNI TGGCCA 1 cut(s) 174
MmeI TCCRAC 1 cut(s) 436
MnlI CCTC 3 cut(s) 51, 325, 542
Mox20I TGGCCA 1 cut(s) 174
MroXI GAANNNNTTC 1 cut(s) 73
MscI TGGCCA 1 cut(s) 174
MseI TTAA 1 cut(s) 587
Msp20I TGGCCA 1 cut(s) 174
MspI CCGG 2 cut(s) 109, 357
MspR9I CCNGG 3 cut(s) 109, 357, 641
MunI CAATTG 2 cut(s) 442, 710
MvaI CCWGG 1 cut(s) 641
MwoI GCNNNNNNNGC 1 cut(s) 180
NciI CCSGG 2 cut(s) 109, 357
NdeI CATATG 1 cut(s) 185
NdeII GATC 5 cut(s) 104, 310, 334, 525, 705
NlaIII CATG 3 cut(s) 120, 156, 378
NlaIV GGNNCC 4 cut(s) 43, 113, 162, 223
PdmI GAANNNNTTC 1 cut(s) 73
PfeI GAWTC 1 cut(s) 74
PkrI GCNGC 6 cut(s) 6, 9, 122, 262, 520, 559
Ppu21I YACGTR 1 cut(s) 687
Psp6I CCWGG 1 cut(s) 639
PspGI CCWGG 1 cut(s) 639
PspN4I GGNNCC 4 cut(s) 43, 113, 162, 223
PspOMI GGGCCC 1 cut(s) 221
PspPI GGNCC 3 cut(s) 41, 221, 222
PsuI RGATCY 1 cut(s) 104
RsaI GTAC 4 cut(s) 323, 638, 685, 689
RsaNI GTAC 4 cut(s) 322, 637, 684, 688
SaqAI TTAA 1 cut(s) 587
SatI GCNGC 6 cut(s) 5, 8, 121, 261, 519, 558
Sau3AI GATC 5 cut(s) 104, 310, 334, 525, 705
Sau96I GGNCC 3 cut(s) 41, 221, 222
ScrFI CCNGG 3 cut(s) 109, 357, 641
SduI GDGCHC 4 cut(s) 51, 225, 401, 554
SfaNI GCATC 1 cut(s) 373
SmlI CTYRAG 2 cut(s) 55, 533
SmoI CTYRAG 2 cut(s) 55, 533
SnaBI TACGTA 1 cut(s) 687
Sse9I AATT 6 cut(s) 15, 90, 226, 407, 442, 710
SsiI CCGC 1 cut(s) 654
SspI AATATT 1 cut(s) 594
StyD4I CCNGG 3 cut(s) 107, 355, 639
TaaI ACNGT 2 cut(s) 582, 692
TaiI ACGT 2 cut(s) 638, 689
TaqII GACCGA 1 cut(s) 477
TasI AATT 6 cut(s) 15, 90, 226, 407, 442, 710
TfiI GAWTC 1 cut(s) 74
Tru1I TTAA 1 cut(s) 587
Tru9I TTAA 1 cut(s) 587
TseI GCWGC 6 cut(s) 4, 7, 120, 260, 518, 557
TspDTI ATGAA 2 cut(s) 141, 366
XapI RAATTY 1 cut(s) 407
XmnI GAANNNNTTC 1 cut(s) 73
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.