AT3G53050

beta-galactosidase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0017500
Physical Location & Seq
Forward (+)
19669084 .. 19669774
691 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G53050.1

Sequence Viewer

Length: 429 bp
ATGGGAGACTCACATTGCCGTTGTCTTCATGGCTTTATTTTGCTTGTTGTTCTGTTTCTTTCCTTCGTGTTTAGTTTCGCTTCAAAGATAGACGTTTCTTTTCACGGAGACGAGAAACGTAGTCTCTCTAGCTCCATTAATTCCTCTCTTAGTGAGTCAGCGGAATACACAATGTGCGCGACTCATAAAGAGGCTAAGGATGGTAAACCAATCGCATTGGATTTCGATTGCGAACAAGGATATGTTATTTCAAAGATCACTTACGCCGACTATGGCCAATCCACTGGTAGTTGTGGGAAATTTAAACGCGGTAATTGCGGTGCAAGCAATACCTTAAATATCGTCAACAAGAAATGTCTTAGGAAAGAGAAGTGTAAGTTGTTTGTTCCAGACAAGATTTTTGGTCCGAGCCACTGCAAGGGAGCTTAA

Protein Analysis

142

Amino Acids

15.55

Weight (kDa)

8.72

Isoelectric Point (pI)

33.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SUEL_Lectin PF02140 76 - 141 4.7e-14 D-galactoside/L-rhamnose binding SUEL lectin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017500)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G53050 AT3G53065 AT3G53070 AT3G53075 AT3G53075 AT3G53080
rosa_chinensis RchiOBHm_Chr2g0162671 RchiOBHm_Chr6g0266221
rosa_multiflora Rmu_co8401661.1_g000001 Rmu_sc0013794.1_g000001
rosa_rugosa Rorug02G0052700
rosa_samantha Rh2BG102300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 179, 309
AciI CCGC 3 cut(s) 161, 309, 318
AcoI YGGCCR 1 cut(s) 274
AcsI RAATTY 1 cut(s) 299
AdeI CACNNNGTG 1 cut(s) 174
AfiI CCNNNNNNNGG 1 cut(s) 418
AgsI TTSAA 2 cut(s) 84, 252
AluBI AGCT 2 cut(s) 132, 425
AluI AGCT 2 cut(s) 132, 425
Alw26I GTCTC 2 cut(s) 102, 128
AoxI GGCC 1 cut(s) 274
ApoI RAATTY 1 cut(s) 299
ArsI GACNNNNNNTTYG 2 cut(s) 383, 415
AseI ATTAAT 1 cut(s) 138
AspLEI GCGC 1 cut(s) 179
AspS9I GGNCC 1 cut(s) 404
AvaII GGWCC 1 cut(s) 404
BalI TGGCCA 1 cut(s) 276
BbsI GAAGAC 1 cut(s) 17
BccI CCATC 1 cut(s) 194
BceAI ACGGC 1 cut(s) 3
BcoDI GTCTC 2 cut(s) 102, 128
BfaI CTAG 1 cut(s) 129
Bme18I GGWCC 1 cut(s) 404
BmgT120I GGNCC 1 cut(s) 404
BpiI GAAGAC 1 cut(s) 17
Bpu10I CCTNAGC 1 cut(s) 195
Bsc4I CCNNNNNNNGG 1 cut(s) 418
Bse1I ACTGG 1 cut(s) 289
Bse3DI GCAATG 1 cut(s) 13
BseGI GGATG 1 cut(s) 205
BseLI CCNNNNNNNGG 1 cut(s) 418
BseMI GCAATG 1 cut(s) 13
BseNI ACTGG 1 cut(s) 289
Bsh1236I CGCG 2 cut(s) 179, 309
BshFI GGCC 1 cut(s) 276
BslI CCNNNNNNNGG 1 cut(s) 418
BsmAI GTCTC 2 cut(s) 102, 128
BsmBI CGTCTC 1 cut(s) 102
BsnI GGCC 1 cut(s) 276
Bsp143I GATC 1 cut(s) 255
BspACI CCGC 3 cut(s) 161, 309, 318
BspANI GGCC 1 cut(s) 276
BspFNI CGCG 2 cut(s) 179, 309
BsrDI GCAATG 1 cut(s) 13
BsrI ACTGG 1 cut(s) 289
BssMI GATC 1 cut(s) 255
BstC8I GCNNGC 1 cut(s) 325
BstDEI CTNAG 3 cut(s) 149, 195, 359
BstF5I GGATG 1 cut(s) 205
BstFNI CGCG 2 cut(s) 179, 309
BstHHI GCGC 1 cut(s) 179
BstKTI GATC 1 cut(s) 258
BstMAI GTCTC 2 cut(s) 102, 128
BstMBI GATC 1 cut(s) 255
BstMWI GCNNNNNNNGC 2 cut(s) 315, 324
BstUI CGCG 2 cut(s) 179, 309
BstV2I GAAGAC 1 cut(s) 17
BstXI CCANNNNNNTGG 1 cut(s) 284
BsuRI GGCC 1 cut(s) 276
BtsCI GGATG 1 cut(s) 205
BtsI GCAGTG 1 cut(s) 412
BtsIMutI CAGTG 2 cut(s) 282, 412
Cac8I GCNNGC 1 cut(s) 325
CfoI GCGC 1 cut(s) 179
Cfr13I GGNCC 1 cut(s) 404
CviAII CATG 1 cut(s) 29
CviJI RGCY 6 cut(s) 33, 132, 194, 276, 411, 425
CviKI_1 RGCY 6 cut(s) 33, 132, 194, 276, 411, 425
DdeI CTNAG 3 cut(s) 149, 195, 359
DpnI GATC 1 cut(s) 257
DpnII GATC 1 cut(s) 255
DraI TTTAAA 1 cut(s) 304
DraIII CACNNNGTG 1 cut(s) 174
EaeI YGGCCR 1 cut(s) 274
Eco47I GGWCC 1 cut(s) 404
Esp3I CGTCTC 1 cut(s) 102
FaeI CATG 1 cut(s) 32
FaiI YATR 4 cut(s) 30, 186, 243, 273
FatI CATG 1 cut(s) 28
FokI GGATG 1 cut(s) 212
FspBI CTAG 1 cut(s) 129
GlaI GCGC 1 cut(s) 178
HaeIII GGCC 1 cut(s) 276
HhaI GCGC 1 cut(s) 179
Hin1II CATG 1 cut(s) 32
Hin6I GCGC 1 cut(s) 177
HinP1I GCGC 1 cut(s) 177
HincII GTYRAC 1 cut(s) 346
HindII GTYRAC 1 cut(s) 346
HinfI GANTC 3 cut(s) 8, 155, 181
Hpy166II GTNNAC 2 cut(s) 206, 346
Hpy188I TCNGA 1 cut(s) 408
Hpy188III TCNNGA 1 cut(s) 389
Hpy8I GTNNAC 2 cut(s) 206, 346
HpyAV CCTTC 1 cut(s) 73
HpyCH4IV ACGT 2 cut(s) 93, 118
HpyCH4V TGCA 2 cut(s) 323, 417
HpyF10VI GCNNNNNNNGC 2 cut(s) 315, 324
HpyF3I CTNAG 3 cut(s) 149, 195, 359
HpySE526I ACGT 2 cut(s) 93, 118
Hsp92II CATG 1 cut(s) 32
HspAI GCGC 1 cut(s) 177
Kzo9I GATC 1 cut(s) 255
LmnI GCTCC 2 cut(s) 137, 422
LpnPI CCDG 2 cut(s) 270, 402
MaeI CTAG 1 cut(s) 129
MaeII ACGT 2 cut(s) 93, 118
MalI GATC 1 cut(s) 257
MboI GATC 1 cut(s) 255
MboII GAAGA 1 cut(s) 17
MlsI TGGCCA 1 cut(s) 276
MluCI AATT 3 cut(s) 139, 299, 313
MluNI TGGCCA 1 cut(s) 276
MlyI GAGTC 3 cut(s) 2, 164, 175
MnlI CCTC 2 cut(s) 154, 184
Mox20I TGGCCA 1 cut(s) 276
MscI TGGCCA 1 cut(s) 276
MseI TTAA 4 cut(s) 138, 303, 335, 427
Msp20I TGGCCA 1 cut(s) 276
MspA1I CMGCKG 1 cut(s) 161
MvnI CGCG 2 cut(s) 179, 309
MwoI GCNNNNNNNGC 2 cut(s) 315, 324
NdeII GATC 1 cut(s) 255
NlaIII CATG 1 cut(s) 32
PleI GAGTC 3 cut(s) 2, 163, 175
PpsI GAGTC 3 cut(s) 2, 163, 175
PshBI ATTAAT 1 cut(s) 138
PspPI GGNCC 1 cut(s) 404
SaqAI TTAA 4 cut(s) 138, 303, 335, 427
Sau3AI GATC 1 cut(s) 255
Sau96I GGNCC 1 cut(s) 404
SchI GAGTC 3 cut(s) 2, 164, 175
SetI ASST 5 cut(s) 96, 121, 134, 335, 427
SinI GGWCC 1 cut(s) 404
Sse9I AATT 3 cut(s) 139, 299, 313
SsiI CCGC 3 cut(s) 161, 309, 318
SspMI CTAG 1 cut(s) 129
TaiI ACGT 2 cut(s) 96, 121
TaqI TCGA 1 cut(s) 225
TasI AATT 3 cut(s) 139, 299, 313
Tru1I TTAA 4 cut(s) 138, 303, 335, 427
Tru9I TTAA 4 cut(s) 138, 303, 335, 427
TscAI CASTG 2 cut(s) 289, 419
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 120
TspRI CASTG 2 cut(s) 289, 419
VpaK11BI GGWCC 1 cut(s) 404
VspI ATTAAT 1 cut(s) 138
XapI RAATTY 1 cut(s) 299
XspI CTAG 1 cut(s) 129
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.