Rorug02G0052700

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0017500
Physical Location & Seq
Reverse (-)
4023587 .. 4026228
2642 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0052700.1

Sequence Viewer

Length: 1128 bp
ATGGACGACATGGAAGACGATGAAAGGTACCACCCGAAACCCTACAACCATCACAGCTCCACGAATCGGCGAAAGATCCACACCCGGAGCAACCCGTATTCCCGGCCACCTCCCAATCGATACGAGCCGGAAGACGACGACGACGCAATCGACAACGATCTGGACGAGGAGAATTACGGCCAAGACGACGTCGTCGTCGTGGAAGAGCAGTACCAGCACCACCCGCACGACCTGAATCGGAGCTTCGACGCCCCCGCCGAGAACGGCTTCGAGTGGCACCCGAAGAAGAGGAAGCTGAAGAATCTGGATTCGGGTTACGAGTTCGCGCCTCGGGTCAAGTTTCCGGGTCGGGTCGGGTCGAGCTCGGAGGAGTGGACGGAGCACGCGGTGTTTGTGCTGCTGGAGGTTTGGGGGGACAGGTTTCTTGAGCTGGGGAGGAAGAGCTTGAGGTCGGAGGACTGGAGTGACGTGGCGGTGAAGGTATCGGAGGGGAGCAAGTTGGAGAGGAATGATAAGCAATGTAGGAAAATGCTGGATATGCTGAAGAGGAAGTATAAGAAGGAGAAGGCGAAAATCGAAGAAGCAGGGGGTTCGAGCAAGTGGGGGTATTTCAAGAAGATGGAAATGCTGATGGCCTCGAATATGAGGCAGGAATGTGGGTTGGCTTGTGGGGTGGATAATGGGGAGTATGTGTTTATGAATACGAGGGTGTATTTGGAGAAGTCGAATGGGTTTGATGAGATGAGGGATAGTCCAGGGGAGTCGGAGACGGATGAGGATGAGAATGAGAGTGAGCAGAATGGGTTTGGGTTCGGGATTGGGATTCCTCCGAGGCAGGGAGGGGGAGGGGGAGGGGGGTTGGAAGGGTCTTCGTTCCGAGTGTTGGCGGATTCTATTCAGAAGTTTGGGGAGATATATGAGAATATTGAGAGTAGTAAGAGGCAGCAGATGATGGAGCTGGAGAAGATGAGGAGGGATTTTAATAAGGAAATGGAGTTGCAGAAGAAGCAGATTCTTGAGAGGGCACAGGCGGAGATTGCGAAGATTCAGGAAGCGGATGATGAGGATGAGGATGAGGATGATGATGACGACGACTCGGATGCTTCTGCTGAAGATCTGAGCGAATGA

Protein Analysis

375

Amino Acids

43.3

Weight (kDa)

4.68

Isoelectric Point (pI)

53.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-bind_4 PF13837 123 - 209 6.4e-16 Myb/SANT-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017500)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G53050 AT3G53065 AT3G53070 AT3G53075 AT3G53075 AT3G53080
rosa_chinensis RchiOBHm_Chr2g0162671 RchiOBHm_Chr6g0266221
rosa_multiflora Rmu_co8401661.1_g000001 Rmu_sc0013794.1_g000001
rosa_rugosa Rorug02G0052700
rosa_samantha Rh2BG102300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 191, 194
AatII GACGTC 1 cut(s) 192
Acc65I GGTACC 1 cut(s) 27
AccB1I GGYRCC 2 cut(s) 27, 276
AccII CGCG 2 cut(s) 326, 386
AciI CCGC 7 cut(s) 224, 255, 386, 473, 887, 1031, 1055
AclWI GGATC 1 cut(s) 70
AcoI YGGCCR 2 cut(s) 104, 178
AcuI CTGAAG 2 cut(s) 317, 563
AcyI GRCGYC 2 cut(s) 189, 249
AdeI CACNNNGTG 1 cut(s) 388
AfaI GTAC 2 cut(s) 29, 212
AfiI CCNNNNNNNGG 3 cut(s) 66, 836, 883
AgsI TTSAA 1 cut(s) 613
AjiI CACGTC 1 cut(s) 469
AjnI CCWGG 1 cut(s) 754
AluBI AGCT 7 cut(s) 57, 243, 295, 363, 430, 444, 958
AluI AGCT 7 cut(s) 57, 243, 295, 363, 430, 444, 958
Alw21I GWGCWC 2 cut(s) 365, 384
Alw26I GTCTC 1 cut(s) 761
AlwI GGATC 1 cut(s) 70
Ama87I CYCGRG 1 cut(s) 330
AoxI GGCC 3 cut(s) 104, 178, 633
ApeKI GCWGC 2 cut(s) 397, 943
Asp700I GAANNNNTTC 1 cut(s) 266
Asp718I GGTACC 1 cut(s) 27
AspLEI GCGC 1 cut(s) 328
AsuC2I CCSGG 3 cut(s) 85, 103, 345
AsuHPI GGTGA 1 cut(s) 487
AvaI CYCGRG 1 cut(s) 330
BaeGI GKGCMC 1 cut(s) 1027
BanI GGYRCC 2 cut(s) 27, 276
BanII GRGCYC 1 cut(s) 365
BbsI GAAGAC 3 cut(s) 21, 138, 861
Bbv12I GWGCWC 2 cut(s) 365, 384
BbvI GCAGC 2 cut(s) 384, 955
BccI CCATC 4 cut(s) 57, 613, 625, 946
BceAI ACGGC 2 cut(s) 193, 280
BcgI CGANNNNNNTGC 4 cut(s) 573, 607, 1082, 1116
BciT130I CCWGG 1 cut(s) 756
BcnI CCSGG 3 cut(s) 85, 103, 345
BcoDI GTCTC 1 cut(s) 761
BglII AGATCT 1 cut(s) 1114
BisI GCNGC 2 cut(s) 398, 944
BlsI GCNGC 2 cut(s) 399, 945
Bme1390I CCNGG 4 cut(s) 85, 103, 345, 756
BmeT110I CYCGRG 1 cut(s) 330
BmgBI CACGTC 1 cut(s) 469
BmiI GGNNCC 2 cut(s) 29, 278
BmrFI CCNGG 4 cut(s) 85, 103, 345, 756
BmsI GCATC 1 cut(s) 1090
BpiI GAAGAC 3 cut(s) 21, 138, 861
BpmI CTGGAG 3 cut(s) 422, 481, 980
BpuEI CTTGAG 3 cut(s) 446, 466, 1037
BpuMI CCSGG 3 cut(s) 85, 103, 345
Bsa29I ATCGAT 1 cut(s) 118
BsaHI GRCGYC 2 cut(s) 189, 249
BsaJI CCNNGG 3 cut(s) 329, 755, 830
BsaXI ACNNNNNCTCC 2 cut(s) 677, 707
Bsc4I CCNNNNNNNGG 3 cut(s) 66, 836, 883
Bse1I ACTGG 1 cut(s) 464
Bse3DI GCAATG 1 cut(s) 524
BseBI CCWGG 1 cut(s) 756
BseCI ATCGAT 1 cut(s) 118
BseDI CCNNGG 3 cut(s) 329, 755, 830
BseGI GGATG 7 cut(s) 778, 784, 1063, 1072, 1078, 1084, 1105
BseLI CCNNNNNNNGG 3 cut(s) 66, 836, 883
BseMI GCAATG 1 cut(s) 524
BseMII CTCAG 1 cut(s) 1109
BseNI ACTGG 1 cut(s) 464
BseRI GAGGAG 3 cut(s) 182, 383, 985
BseSI GKGCMC 1 cut(s) 1027
BseXI GCAGC 2 cut(s) 384, 955
BseYI CCCAGC 1 cut(s) 430
Bsh1236I CGCG 2 cut(s) 326, 386
BshFI GGCC 3 cut(s) 106, 180, 635
BshNI GGYRCC 2 cut(s) 27, 276
BshVI ATCGAT 1 cut(s) 118
BsiHKAI GWGCWC 2 cut(s) 365, 384
BsiHKCI CYCGRG 1 cut(s) 330
BsiSI CCGG 4 cut(s) 85, 103, 128, 344
BslFI GGGAC 1 cut(s) 428
BslI CCNNNNNNNGG 3 cut(s) 66, 836, 883
BsmAI GTCTC 1 cut(s) 761
BsmBI CGTCTC 1 cut(s) 761
BsmFI GGGAC 1 cut(s) 428
BsnI GGCC 3 cut(s) 106, 180, 635
BsoBI CYCGRG 1 cut(s) 330
Bsp1286I GDGCHC 3 cut(s) 365, 384, 1027
Bsp143I GATC 3 cut(s) 75, 157, 1114
BspACI CCGC 7 cut(s) 224, 255, 386, 473, 887, 1031, 1055
BspANI GGCC 3 cut(s) 106, 180, 635
BspCNI CTCAG 1 cut(s) 1110
BspDI ATCGAT 1 cut(s) 118
BspFNI CGCG 2 cut(s) 326, 386
BspLI GGNNCC 2 cut(s) 29, 278
BspPI GGATC 1 cut(s) 70
BspQI GCTCTTC 2 cut(s) 198, 434
BspT107I GGYRCC 2 cut(s) 27, 276
BsrDI GCAATG 1 cut(s) 524
BsrI ACTGG 1 cut(s) 464
BssECI CCNNGG 3 cut(s) 329, 755, 830
BssMI GATC 3 cut(s) 75, 157, 1114
BssNI GRCGYC 2 cut(s) 189, 249
Bst2UI CCWGG 1 cut(s) 756
Bst6I CTCTTC 4 cut(s) 198, 281, 434, 539
BstACI GRCGYC 2 cut(s) 189, 249
BstC8I GCNNGC 1 cut(s) 384
BstDEI CTNAG 1 cut(s) 1118
BstF5I GGATG 7 cut(s) 778, 784, 1063, 1072, 1078, 1084, 1105
BstFNI CGCG 2 cut(s) 326, 386
BstHHI GCGC 1 cut(s) 328
BstKTI GATC 3 cut(s) 78, 160, 1117
BstMAI GTCTC 1 cut(s) 761
BstMBI GATC 3 cut(s) 75, 157, 1114
BstMWI GCNNNNNNNGC 5 cut(s) 214, 223, 538, 1006, 1037
BstNI CCWGG 1 cut(s) 756
BstSCI CCNGG 4 cut(s) 83, 101, 343, 754
BstSLI GKGCMC 1 cut(s) 1027
BstUI CGCG 2 cut(s) 326, 386
BstV1I GCAGC 2 cut(s) 384, 955
BstV2I GAAGAC 3 cut(s) 21, 138, 861
BstX2I RGATCY 2 cut(s) 75, 1114
BstYI RGATCY 2 cut(s) 75, 1114
Bsu15I ATCGAT 1 cut(s) 118
BsuRI GGCC 3 cut(s) 106, 180, 635
BsuTUI ATCGAT 1 cut(s) 118
BtrI CACGTC 1 cut(s) 469
BtsCI GGATG 7 cut(s) 778, 784, 1063, 1072, 1078, 1084, 1105
Cac8I GCNNGC 1 cut(s) 384
CfoI GCGC 1 cut(s) 328
ClaI ATCGAT 1 cut(s) 118
CseI GACGC 2 cut(s) 152, 257
Csp6I GTAC 2 cut(s) 28, 211
CviAII CATG 1 cut(s) 10
CviQI GTAC 2 cut(s) 28, 211
DdeI CTNAG 1 cut(s) 1118
DpnI GATC 3 cut(s) 77, 159, 1116
DpnII GATC 3 cut(s) 75, 157, 1114
DraIII CACNNNGTG 1 cut(s) 388
DrdI GACNNNNNNGTC 2 cut(s) 191, 194
DseDI GACNNNNNNGTC 2 cut(s) 191, 194
EaeI YGGCCR 2 cut(s) 104, 178
Eam1104I CTCTTC 4 cut(s) 198, 281, 434, 539
EarI CTCTTC 4 cut(s) 198, 281, 434, 539
EciI GGCGGA 2 cut(s) 902, 1046
Ecl136II GAGCTC 1 cut(s) 363
Eco24I GRGCYC 1 cut(s) 365
Eco53kI GAGCTC 1 cut(s) 363
Eco57I CTGAAG 2 cut(s) 317, 563
Eco88I CYCGRG 1 cut(s) 330
EcoICRI GAGCTC 1 cut(s) 363
EcoRII CCWGG 1 cut(s) 754
EcoT38I GRGCYC 1 cut(s) 365
Esp3I CGTCTC 1 cut(s) 761
FaeI CATG 1 cut(s) 13
FaiI YATR 8 cut(s) 11, 539, 555, 644, 690, 698, 916, 918
FaqI GGGAC 1 cut(s) 428
FatI CATG 1 cut(s) 9
FauI CCCGC 2 cut(s) 231, 262
Fnu4HI GCNGC 2 cut(s) 398, 944
FokI GGATG 7 cut(s) 785, 791, 1070, 1079, 1085, 1091, 1112
FriOI GRGCYC 1 cut(s) 365
Fsp4HI GCNGC 2 cut(s) 398, 944
GlaI GCGC 1 cut(s) 327
GluI GCNGC 2 cut(s) 398, 944
GsaI CCCAGC 1 cut(s) 434
GsuI CTGGAG 3 cut(s) 422, 481, 980
HaeIII GGCC 3 cut(s) 106, 180, 635
HapII CCGG 4 cut(s) 85, 103, 128, 344
HgaI GACGC 2 cut(s) 152, 257
HhaI GCGC 1 cut(s) 328
Hin1I GRCGYC 2 cut(s) 189, 249
Hin1II CATG 1 cut(s) 13
Hin6I GCGC 1 cut(s) 326
HinP1I GCGC 1 cut(s) 326
HpaII CCGG 4 cut(s) 85, 103, 128, 344
HphI GGTGA 1 cut(s) 487
Hpy166II GTNNAC 1 cut(s) 375
Hpy188III TCNNGA 7 cut(s) 161, 305, 425, 613, 814, 1016, 1049
Hpy8I GTNNAC 1 cut(s) 375
Hpy99I CGWCG 9 cut(s) 140, 143, 146, 191, 194, 197, 200, 251, 1094
HpyAV CCTTC 4 cut(s) 472, 553, 559, 857
HpyCH4IV ACGT 2 cut(s) 189, 468
HpyCH4V TGCA 1 cut(s) 1000
HpyF10VI GCNNNNNNNGC 5 cut(s) 214, 223, 538, 1006, 1037
HpyF3I CTNAG 1 cut(s) 1118
HpySE526I ACGT 2 cut(s) 189, 468
Hsp92I GRCGYC 2 cut(s) 189, 249
Hsp92II CATG 1 cut(s) 13
HspAI GCGC 1 cut(s) 326
KpnI GGTACC 1 cut(s) 31
Kzo9I GATC 3 cut(s) 75, 157, 1114
LguI GCTCTTC 2 cut(s) 198, 434
LmnI GCTCC 6 cut(s) 62, 87, 240, 379, 492, 955
Lsp1109I GCAGC 2 cut(s) 384, 955
LweI GCATC 1 cut(s) 1090
MaeII ACGT 2 cut(s) 189, 468
MaeIII GTNAC 2 cut(s) 314, 464
MalI GATC 3 cut(s) 77, 159, 1116
MboI GATC 3 cut(s) 75, 157, 1114
MflI RGATCY 2 cut(s) 75, 1114
MhlI GDGCHC 3 cut(s) 365, 384, 1027
MluCI AATT 1 cut(s) 172
MlyI GAGTC 2 cut(s) 770, 1088
MmeI TCCRAC 4 cut(s) 432, 480, 744, 840
MroXI GAANNNNTTC 1 cut(s) 266
MseI TTAA 1 cut(s) 981
MspI CCGG 4 cut(s) 85, 103, 128, 344
MspR9I CCNGG 4 cut(s) 85, 103, 345, 756
MvaI CCWGG 1 cut(s) 756
MvnI CGCG 2 cut(s) 326, 386
MwoI GCNNNNNNNGC 5 cut(s) 214, 223, 538, 1006, 1037
NciI CCSGG 3 cut(s) 85, 103, 345
NdeII GATC 3 cut(s) 75, 157, 1114
NlaIII CATG 1 cut(s) 13
NlaIV GGNNCC 2 cut(s) 29, 278
NmeAIII GCCGAG 1 cut(s) 283
NmuCI GTSAC 1 cut(s) 464
PciSI GCTCTTC 2 cut(s) 198, 434
PcsI WCGNNNNNNNCGW 6 cut(s) 147, 162, 183, 192, 195, 255
PdmI GAANNNNTTC 1 cut(s) 266
PfeI GAWTC 8 cut(s) 64, 235, 301, 308, 823, 890, 1012, 1045
PflFI GACNNNGTC 2 cut(s) 188, 191
PkrI GCNGC 2 cut(s) 399, 945
PleI GAGTC 2 cut(s) 769, 1088
PpsI GAGTC 2 cut(s) 769, 1088
Psp124BI GAGCTC 1 cut(s) 365
Psp6I CCWGG 1 cut(s) 754
PspFI CCCAGC 1 cut(s) 430
PspGI CCWGG 1 cut(s) 754
PspN4I GGNNCC 2 cut(s) 29, 278
PsuI RGATCY 2 cut(s) 75, 1114
PsyI GACNNNGTC 2 cut(s) 188, 191
RsaI GTAC 2 cut(s) 29, 212
RsaNI GTAC 2 cut(s) 28, 211
SacI GAGCTC 1 cut(s) 365
SapI GCTCTTC 2 cut(s) 198, 434
SaqAI TTAA 1 cut(s) 981
SatI GCNGC 2 cut(s) 398, 944
Sau3AI GATC 3 cut(s) 75, 157, 1114
SchI GAGTC 2 cut(s) 770, 1088
ScrFI CCNGG 4 cut(s) 85, 103, 345, 756
SduI GDGCHC 3 cut(s) 365, 384, 1027
SfaNI GCATC 1 cut(s) 1090
SmlI CTYRAG 3 cut(s) 425, 445, 1016
SmoI CTYRAG 3 cut(s) 425, 445, 1016
Sse9I AATT 1 cut(s) 172
SsiI CCGC 7 cut(s) 224, 255, 386, 473, 887, 1031, 1055
SspI AATATT 1 cut(s) 925
SstI GAGCTC 1 cut(s) 365
StyD4I CCNGG 4 cut(s) 83, 101, 343, 754
TaiI ACGT 2 cut(s) 192, 471
TaqI TCGA 9 cut(s) 118, 150, 246, 270, 359, 576, 593, 638, 725
TasI AATT 1 cut(s) 172
TfiI GAWTC 8 cut(s) 64, 235, 301, 308, 823, 890, 1012, 1045
Tru1I TTAA 1 cut(s) 981
Tru9I TTAA 1 cut(s) 981
TseFI GTSAC 1 cut(s) 464
TseI GCWGC 2 cut(s) 397, 943
Tsp45I GTSAC 1 cut(s) 464
TspDTI ATGAA 2 cut(s) 36, 713
TspGWI ACGGA 2 cut(s) 392, 785
Tth111I GACNNNGTC 2 cut(s) 188, 191
XmnI GAANNNNTTC 1 cut(s) 266
ZraI GACGTC 1 cut(s) 190
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.