AT3G53520

udp-glucuronic acid decarboxylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0008196
Physical Location & Seq
Forward (+)
19841374 .. 19844412
3039 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G53520.2

Sequence Viewer

Length: 1308 bp
ATGAAGCAGCTTCACAAGCAAATGAGCTCGAAGCGTGATGAAGAAACCATTCCCATGAGCCAATCCAGTCCTTACTCTCCCAAAACTTTAAAGCATCCCAGATCTCTTCCCAGATCGCTTCACTATCTCTTTCGTGAACAACGACTCCTCTTTATCCTCGTCGGGATCTTGATCGGATCCACTTTCTTCATCCTCCAGCCTTCTCTCTCTCGTTTAGGCGCCGCTGAGTCCACCTCGTTAATCACCAGATCTGTTTCCTACGCCGTCACCGATTCCCCGCCTTCCAGGTCGACTTTTAATTCCGGAGGCGGCGGAGGTAGAACCGGTCGTGTTCCCGTCGGTATCGGACGGAAGAGACTGAGGATTGTTGTCACCGGTGGAGCTGGTTTCGTCGGTAGTCATCTTGTTGATAAGCTTATCGGTAGGGGAGATGAAGTGATCGTGATTGATAACTTCTTCACTGGTAGGAAGGAGAATTTGGTTCATCTATTCTCGAATCCTAGGTTTGAGCTAATTCGACACGATGTTGTTGAGCCAATCCTCCTTGAGGTCGATCAGATTTACCATTTAGCTTGTCCAGCTTCACCTGTTCATTACAAGTATAATCCAGTCAAGACTATCAAGACAAATGTAATGGGCACTCTCAATATGTTGGGTCTTGCAAAGAGAGTTGGGGCAAGGTTTCTGCTCACCAGCACAAGTGAAGTCTATGGAGATCCCCTTGAGCATCCACAGAAAGAGACTTACTGGGGAAACGTGAATCCAATCGGTGAGAGGAGTTGCTATGATGAAGGAAAGCGTACTGCAGAAACCCTGGCCATGGATTATCACCGAGGTGCAGGTGTGGAGGTTAGAATTGCTCGTATTTTCAACACATATGGGCCCAGAATGTGCCTTGATGATGGGCGTGTTGTTAGTAACTTTGTTGCCCAGACCATTCGCAAACACCCAATGACTGTTTATGGTGATGGAAAACAGACTCGTAGCTTTCAATATGTTTCTGACTTGGTGGAGGGACTTGTAGCTTTAATGGAAAACGATCATGTAGGACCCTTCAACCTTGGTAACCCTGGAGAATTCACAATGCTTGAACTTGCAGAGGTGGTTAAGGAGGTGATCGACCCAAGCGCGACCATAGAGTTCAAACCAAACACAGCAGACGATCCTCACAAGAGGAAACCAGACATAAGCAAAGCAAAGGAACAGTTGAACTGGGAACCAAAGATATCTCTGAGGGAGGGACTGCCTCGCATGGTTAGTGACTTCCGTAACCGAATCTTAAACGAAGACGAAGGTAAAGGTCTCTAA

Protein Analysis

435

Amino Acids

48.63

Weight (kDa)

9.21

Isoelectric Point (pI)

42.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RmlD_sub_bind PF04321 121 - 419 3.6e-07 RmlD substrate binding domain
Epimerase PF01370 122 - 352 3.1e-44 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 123 - 334 6e-13 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 124 - 416 3.3e-54 GDP-mannose 4,6 dehydratase
NAD_binding_4 PF07993 177 - 289 1.1e-06 Male sterility protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 830
Acc36I ACCTGC 1 cut(s) 830
AccB1I GGYRCC 1 cut(s) 218
AccI GTMKAC 1 cut(s) 290
AccII CGCG 1 cut(s) 1130
AccIII TCCGGA 1 cut(s) 302
AciI CCGC 4 cut(s) 222, 278, 309, 312
AclWI GGATC 5 cut(s) 171, 173, 184, 710, 1157
AcoI YGGCCR 1 cut(s) 816
AcsI RAATTY 2 cut(s) 475, 1076
AcyI GRCGYC 1 cut(s) 219
AfaI GTAC 1 cut(s) 802
AfiI CCNNNNNNNGG 2 cut(s) 547, 820
AgeI ACCGGT 2 cut(s) 323, 374
AgsI TTSAA 6 cut(s) 871, 992, 1057, 1091, 1144, 1210
AjnI CCWGG 3 cut(s) 284, 813, 1069
AjuI GAANNNNNNNTTGG 2 cut(s) 461, 493
AleI CACNNNNGTG 1 cut(s) 834
AloI GAACNNNNNNTCC 2 cut(s) 129, 161
AluBI AGCT 9 cut(s) 10, 27, 383, 415, 511, 572, 581, 987, 1025
AluI AGCT 9 cut(s) 10, 27, 383, 415, 511, 572, 581, 987, 1025
Alw21I GWGCWC 1 cut(s) 29
Alw26I GTCTC 2 cut(s) 349, 734
AlwI GGATC 5 cut(s) 171, 173, 184, 710, 1157
Aor13HI TCCGGA 1 cut(s) 302
AoxI GGCC 2 cut(s) 816, 881
ApaI GGGCCC 1 cut(s) 885
ApeKI GCWGC 1 cut(s) 7
ApoI RAATTY 2 cut(s) 475, 1076
AsiGI ACCGGT 2 cut(s) 323, 374
Asp700I GAANNNNTTC 1 cut(s) 48
AspA2I CCTAGG 1 cut(s) 500
AspLEI GCGC 2 cut(s) 221, 1130
AspS9I GGNCC 3 cut(s) 881, 882, 1049
AsuHPI GGTGA 9 cut(s) 235, 259, 364, 576, 682, 782, 821, 977, 1126
AvaII GGWCC 1 cut(s) 1049
AvrII CCTAGG 1 cut(s) 500
BaeGI GKGCMC 2 cut(s) 641, 885
BalI TGGCCA 1 cut(s) 818
BamHI GGATCC 1 cut(s) 176
BanI GGYRCC 1 cut(s) 218
BanII GRGCYC 2 cut(s) 29, 885
BbsI GAAGAC 1 cut(s) 1293
Bbv12I GWGCWC 1 cut(s) 29
BbvI GCAGC 1 cut(s) 19
BccI CCATC 2 cut(s) 896, 962
BceAI ACGGC 1 cut(s) 248
BciT130I CCWGG 3 cut(s) 286, 815, 1071
BcoDI GTCTC 2 cut(s) 349, 734
BfaI CTAG 1 cut(s) 501
BfmI CTRYAG 1 cut(s) 804
BfoI RGCGCY 1 cut(s) 222
BfuAI ACCTGC 1 cut(s) 830
BglII AGATCT 2 cut(s) 101, 248
BisI GCNGC 3 cut(s) 8, 222, 310
BlnI CCTAGG 1 cut(s) 500
BlsI GCNGC 3 cut(s) 9, 223, 311
Bme1390I CCNGG 3 cut(s) 286, 815, 1071
Bme18I GGWCC 1 cut(s) 1049
BmgT120I GGNCC 3 cut(s) 881, 882, 1049
BmiI GGNNCC 5 cut(s) 178, 220, 883, 1051, 1218
BmrFI CCNGG 3 cut(s) 286, 815, 1071
BmrI ACTGGG 2 cut(s) 757, 1222
BmsI GCATC 2 cut(s) 103, 736
BmuI ACTGGG 2 cut(s) 757, 1222
BpiI GAAGAC 1 cut(s) 1293
BplI GAGNNNNNCTC 2 cut(s) 218, 250
BpmI CTGGAG 2 cut(s) 179, 1092
BpuEI CTTGAG 2 cut(s) 566, 743
BsaBI GATNNNNATC 1 cut(s) 170
BsaHI GRCGYC 1 cut(s) 219
BsaJI CCNNGG 6 cut(s) 500, 813, 819, 832, 1060, 1069
BsaWI WCCGGW 3 cut(s) 302, 323, 374
BsaXI ACNNNNNCTCC 4 cut(s) 129, 159, 420, 450
Bsc4I CCNNNNNNNGG 2 cut(s) 547, 820
Bse118I RCCGGY 2 cut(s) 323, 374
Bse1I ACTGG 5 cut(s) 66, 466, 608, 752, 1217
Bse8I GATNNNNATC 1 cut(s) 170
BseAI TCCGGA 1 cut(s) 302
BseBI CCWGG 3 cut(s) 286, 815, 1071
BseDI CCNNGG 6 cut(s) 500, 813, 819, 832, 1060, 1069
BseGI GGATG 3 cut(s) 94, 189, 727
BseJI GATNNNNATC 1 cut(s) 170
BseLI CCNNNNNNNGG 2 cut(s) 547, 820
BseMII CTCAG 3 cut(s) 216, 350, 1223
BseNI ACTGG 5 cut(s) 66, 466, 608, 752, 1217
BseRI GAGGAG 2 cut(s) 137, 790
BseSI GKGCMC 2 cut(s) 641, 885
BseXI GCAGC 1 cut(s) 19
BsgI GTGCAG 1 cut(s) 858
Bsh1236I CGCG 1 cut(s) 1130
Bsh1285I CGRYCG 1 cut(s) 328
BshFI GGCC 2 cut(s) 818, 883
BshNI GGYRCC 1 cut(s) 218
BshTI ACCGGT 2 cut(s) 323, 374
BsiEI CGRYCG 1 cut(s) 328
BsiHKAI GWGCWC 1 cut(s) 29
BsiSI CCGG 3 cut(s) 303, 324, 375
BslFI GGGAC 2 cut(s) 1029, 1254
BslI CCNNNNNNNGG 2 cut(s) 547, 820
BsmAI GTCTC 2 cut(s) 349, 734
BsmFI GGGAC 2 cut(s) 1029, 1254
BsnI GGCC 2 cut(s) 818, 883
Bsp120I GGGCCC 1 cut(s) 881
Bsp1286I GDGCHC 3 cut(s) 29, 641, 885
Bsp13I TCCGGA 1 cut(s) 302
Bsp19I CCATGG 1 cut(s) 819
BspACI CCGC 4 cut(s) 222, 278, 309, 312
BspANI GGCC 2 cut(s) 818, 883
BspCNI CTCAG 3 cut(s) 217, 351, 1224
BspEI TCCGGA 1 cut(s) 302
BspFNI CGCG 1 cut(s) 1130
BspLI GGNNCC 5 cut(s) 178, 220, 883, 1051, 1218
BspMAI CTGCAG 1 cut(s) 808
BspMI ACCTGC 1 cut(s) 830
BspPI GGATC 5 cut(s) 171, 173, 184, 710, 1157
BspT107I GGYRCC 1 cut(s) 218
BsrFI RCCGGY 2 cut(s) 323, 374
BsrI ACTGG 5 cut(s) 66, 466, 608, 752, 1217
BssAI RCCGGY 2 cut(s) 323, 374
BssECI CCNNGG 6 cut(s) 500, 813, 819, 832, 1060, 1069
BssNI GRCGYC 1 cut(s) 219
BssT1I CCWWGG 3 cut(s) 500, 819, 1060
Bst2UI CCWGG 3 cut(s) 286, 815, 1071
Bst4CI ACNGT 2 cut(s) 958, 1206
Bst6I CTCTTC 2 cut(s) 111, 347
BstACI GRCGYC 1 cut(s) 219
BstDEI CTNAG 3 cut(s) 225, 359, 1232
BstDSI CCRYGG 1 cut(s) 819
BstEII GGTNACC 1 cut(s) 1064
BstENI CCTNNNNNAGG 1 cut(s) 545
BstF5I GGATG 3 cut(s) 94, 189, 727
BstFNI CGCG 1 cut(s) 1130
BstH2I RGCGCY 1 cut(s) 222
BstHHI GCGC 2 cut(s) 221, 1130
BstMAI GTCTC 2 cut(s) 349, 734
BstMCI CGRYCG 1 cut(s) 328
BstMWI GCNNNNNNNGC 2 cut(s) 16, 578
BstNI CCWGG 3 cut(s) 286, 815, 1071
BstPI GGTNACC 1 cut(s) 1064
BstSCI CCNGG 3 cut(s) 284, 813, 1069
BstSFI CTRYAG 1 cut(s) 804
BstSLI GKGCMC 2 cut(s) 641, 885
BstUI CGCG 1 cut(s) 1130
BstV1I GCAGC 1 cut(s) 19
BstV2I GAAGAC 1 cut(s) 1293
BstX2I RGATCY 5 cut(s) 101, 165, 176, 248, 715
BstYI RGATCY 5 cut(s) 101, 165, 176, 248, 715
BsuRI GGCC 2 cut(s) 818, 883
BtgI CCRYGG 1 cut(s) 819
BtsCI GGATG 3 cut(s) 94, 189, 727
BtsIMutI CAGTG 1 cut(s) 459
BveI ACCTGC 1 cut(s) 830
CfoI GCGC 2 cut(s) 221, 1130
Cfr10I RCCGGY 2 cut(s) 323, 374
Cfr13I GGNCC 3 cut(s) 881, 882, 1049
Csp6I GTAC 1 cut(s) 801
CspAI ACCGGT 2 cut(s) 323, 374
CviAII CATG 4 cut(s) 55, 820, 1043, 1252
CviQI GTAC 1 cut(s) 801
DdeI CTNAG 3 cut(s) 225, 359, 1232
DinI GGCGCC 1 cut(s) 220
DraI TTTAAA 1 cut(s) 90
EaeI YGGCCR 1 cut(s) 816
Eam1104I CTCTTC 2 cut(s) 111, 347
EarI CTCTTC 2 cut(s) 111, 347
EciI GGCGGA 1 cut(s) 327
Ecl136II GAGCTC 1 cut(s) 27
Eco130I CCWWGG 3 cut(s) 500, 819, 1060
Eco24I GRGCYC 2 cut(s) 29, 885
Eco32I GATATC 1 cut(s) 1227
Eco47I GGWCC 1 cut(s) 1049
Eco53kI GAGCTC 1 cut(s) 27
Eco91I GGTNACC 1 cut(s) 1064
EcoICRI GAGCTC 1 cut(s) 27
EcoNI CCTNNNNNAGG 1 cut(s) 545
EcoO109I RGGNCCY 1 cut(s) 1049
EcoO65I GGTNACC 1 cut(s) 1064
EcoRI GAATTC 1 cut(s) 1076
EcoRII CCWGG 3 cut(s) 284, 813, 1069
EcoRV GATATC 1 cut(s) 1227
EcoT14I CCWWGG 3 cut(s) 500, 819, 1060
EcoT38I GRGCYC 2 cut(s) 29, 885
EgeI GGCGCC 1 cut(s) 220
EheI GGCGCC 1 cut(s) 220
ErhI CCWWGG 3 cut(s) 500, 819, 1060
FaeI CATG 4 cut(s) 58, 823, 1046, 1255
FaqI GGGAC 2 cut(s) 1029, 1254
FatI CATG 4 cut(s) 54, 819, 1042, 1251
FauI CCCGC 1 cut(s) 285
FauNDI CATATG 1 cut(s) 877
FblI GTMKAC 1 cut(s) 290
Fnu4HI GCNGC 3 cut(s) 8, 222, 310
FokI GGATG 3 cut(s) 81, 176, 714
FriOI GRGCYC 2 cut(s) 29, 885
Fsp4HI GCNGC 3 cut(s) 8, 222, 310
FspBI CTAG 1 cut(s) 501
GlaI GCGC 2 cut(s) 220, 1129
GluI GCNGC 3 cut(s) 8, 222, 310
GsuI CTGGAG 2 cut(s) 179, 1092
HaeII RGCGCY 1 cut(s) 222
HaeIII GGCC 2 cut(s) 818, 883
HapII CCGG 3 cut(s) 303, 324, 375
HhaI GCGC 2 cut(s) 221, 1130
Hin1I GRCGYC 1 cut(s) 219
Hin1II CATG 4 cut(s) 58, 823, 1046, 1255
Hin6I GCGC 2 cut(s) 219, 1128
HinP1I GCGC 2 cut(s) 219, 1128
HincII GTYRAC 1 cut(s) 291
HindII GTYRAC 1 cut(s) 291
HindIII AAGCTT 1 cut(s) 413
HinfI GANTC 7 cut(s) 144, 227, 272, 496, 760, 979, 1275
HpaII CCGG 3 cut(s) 303, 324, 375
HphI GGTGA 9 cut(s) 235, 259, 364, 576, 682, 782, 821, 977, 1126
Hpy166II GTNNAC 3 cut(s) 137, 231, 291
Hpy188I TCNGA 5 cut(s) 176, 347, 558, 1003, 1233
Hpy188III TCNNGA 8 cut(s) 134, 163, 169, 303, 442, 493, 613, 622
Hpy8I GTNNAC 3 cut(s) 137, 231, 291
Hpy99I CGWCG 3 cut(s) 164, 341, 395
HpyAV CCTTC 6 cut(s) 210, 291, 463, 785, 1063, 1286
HpyCH4III ACNGT 2 cut(s) 958, 1206
HpyCH4IV ACGT 1 cut(s) 756
HpyCH4V TGCA 4 cut(s) 662, 806, 839, 1097
HpyF10VI GCNNNNNNNGC 2 cut(s) 16, 578
HpyF3I CTNAG 3 cut(s) 225, 359, 1232
HpySE526I ACGT 1 cut(s) 756
Hsp92I GRCGYC 1 cut(s) 219
Hsp92II CATG 4 cut(s) 58, 823, 1046, 1255
HspAI GCGC 2 cut(s) 219, 1128
KasI GGCGCC 1 cut(s) 218
Kpn2I TCCGGA 1 cut(s) 302
LmnI GCTCC 1 cut(s) 380
Lsp1109I GCAGC 1 cut(s) 19
LweI GCATC 2 cut(s) 103, 736
MaeI CTAG 1 cut(s) 501
MaeII ACGT 1 cut(s) 756
MaeIII GTNAC 6 cut(s) 265, 370, 917, 1064, 1259, 1268
MboII GAAGA 6 cut(s) 53, 98, 178, 364, 448, 1298
MflI RGATCY 5 cut(s) 101, 165, 176, 248, 715
MhlI GDGCHC 3 cut(s) 29, 641, 885
MlsI TGGCCA 1 cut(s) 818
MluCI AATT 5 cut(s) 298, 475, 513, 855, 1076
MluNI TGGCCA 1 cut(s) 818
Mly113I GGCGCC 1 cut(s) 219
MlyI GAGTC 3 cut(s) 138, 236, 973
Mox20I TGGCCA 1 cut(s) 818
MroI TCCGGA 1 cut(s) 302
MroXI GAANNNNTTC 1 cut(s) 48
MscI TGGCCA 1 cut(s) 818
MseI TTAA 6 cut(s) 89, 239, 297, 1028, 1107, 1280
MslI CAYNNNNRTG 2 cut(s) 53, 834
Msp20I TGGCCA 1 cut(s) 818
MspA1I CMGCKG 1 cut(s) 224
MspI CCGG 3 cut(s) 303, 324, 375
MspR9I CCNGG 3 cut(s) 286, 815, 1071
MvaI CCWGG 3 cut(s) 286, 815, 1071
MvnI CGCG 1 cut(s) 1130
MwoI GCNNNNNNNGC 2 cut(s) 16, 578
NarI GGCGCC 1 cut(s) 219
NcoI CCATGG 1 cut(s) 819
NdeI CATATG 1 cut(s) 877
NlaIII CATG 4 cut(s) 58, 823, 1046, 1255
NlaIV GGNNCC 5 cut(s) 178, 220, 883, 1051, 1218
NmuCI GTSAC 3 cut(s) 265, 370, 1259
OliI CACNNNNGTG 1 cut(s) 834
PaqCI CACCTGC 1 cut(s) 830
PcsI WCGNNNNNNNCGW 2 cut(s) 139, 267
PdmI GAANNNNTTC 1 cut(s) 48
PfeI GAWTC 4 cut(s) 272, 496, 760, 1275
PinAI ACCGGT 2 cut(s) 323, 374
PkrI GCNGC 3 cut(s) 9, 223, 311
PleI GAGTC 3 cut(s) 138, 235, 973
PluTI GGCGCC 1 cut(s) 222
PpsI GAGTC 3 cut(s) 138, 235, 973
PpuMI RGGWCCY 1 cut(s) 1049
Psp124BI GAGCTC 1 cut(s) 29
Psp5II RGGWCCY 1 cut(s) 1049
Psp6I CCWGG 3 cut(s) 284, 813, 1069
PspEI GGTNACC 1 cut(s) 1064
PspGI CCWGG 3 cut(s) 284, 813, 1069
PspN4I GGNNCC 5 cut(s) 178, 220, 883, 1051, 1218
PspOMI GGGCCC 1 cut(s) 881
PspPI GGNCC 3 cut(s) 881, 882, 1049
PspPPI RGGWCCY 1 cut(s) 1049
PstI CTGCAG 1 cut(s) 808
PsuI RGATCY 5 cut(s) 101, 165, 176, 248, 715
RsaI GTAC 1 cut(s) 802
RsaNI GTAC 1 cut(s) 801
RseI CAYNNNNRTG 2 cut(s) 53, 834
SacI GAGCTC 1 cut(s) 29
SalI GTCGAC 1 cut(s) 289
SaqAI TTAA 6 cut(s) 89, 239, 297, 1028, 1107, 1280
SatI GCNGC 3 cut(s) 8, 222, 310
Sau96I GGNCC 3 cut(s) 881, 882, 1049
SchI GAGTC 3 cut(s) 138, 236, 973
ScrFI CCNGG 3 cut(s) 286, 815, 1071
SduI GDGCHC 3 cut(s) 29, 641, 885
SfaNI GCATC 2 cut(s) 103, 736
SfcI CTRYAG 1 cut(s) 804
SfoI GGCGCC 1 cut(s) 220
SgrAI CRCCGGYG 1 cut(s) 374
SinI GGWCC 1 cut(s) 1049
SmiMI CAYNNNNRTG 2 cut(s) 53, 834
SmlI CTYRAG 2 cut(s) 545, 722
SmoI CTYRAG 2 cut(s) 545, 722
Sse9I AATT 5 cut(s) 298, 475, 513, 855, 1076
SsiI CCGC 4 cut(s) 222, 278, 309, 312
SspDI GGCGCC 1 cut(s) 218
SspMI CTAG 1 cut(s) 501
SstI GAGCTC 1 cut(s) 29
StyD4I CCNGG 3 cut(s) 284, 813, 1069
StyI CCWWGG 3 cut(s) 500, 819, 1060
TaaI ACNGT 2 cut(s) 958, 1206
TaiI ACGT 1 cut(s) 759
TaqI TCGA 6 cut(s) 29, 290, 494, 517, 552, 1119
TasI AATT 5 cut(s) 298, 475, 513, 855, 1076
TauI GCSGC 2 cut(s) 224, 312
TfiI GAWTC 4 cut(s) 272, 496, 760, 1275
Tru1I TTAA 6 cut(s) 89, 239, 297, 1028, 1107, 1280
Tru9I TTAA 6 cut(s) 89, 239, 297, 1028, 1107, 1280
TscAI CASTG 1 cut(s) 466
TseFI GTSAC 3 cut(s) 265, 370, 1259
TseI GCWGC 1 cut(s) 7
Tsp45I GTSAC 3 cut(s) 265, 370, 1259
TspDTI ATGAA 7 cut(s) 17, 54, 178, 447, 473, 581, 804
TspGWI ACGGA 2 cut(s) 364, 1256
TspRI CASTG 1 cut(s) 466
VpaK11BI GGWCC 1 cut(s) 1049
XagI CCTNNNNNAGG 1 cut(s) 545
XapI RAATTY 2 cut(s) 475, 1076
XmaJI CCTAGG 1 cut(s) 500
XmiI GTMKAC 1 cut(s) 290
XmnI GAANNNNTTC 1 cut(s) 48
XspI CTAG 1 cut(s) 501
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.