AT3G54020

Phosphatidylinositol ceramide inositolphosphotransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0012781
Physical Location & Seq
Forward (+)
20005616 .. 20008254
2639 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G54020.1

Sequence Viewer

Length: 918 bp
ATGACGCTTTATATTCGCCGCGAAGCTTCCAAGCTATGGAGGAGATTTTGTTCGGAAATAACAACGGAGATTGGTCTCCTCGCTGAGAACTGGAAATACCTTCTTGCTGGTCTTCTCTGTCAGTATATTCATGGTTTAGCTGCCAGGGGAGTTCATTATATTCATCGGCCAGGACCAACGCTTCAAGATTCTGGCTTTTTTGTTCTTCCGGAGCTTGGTCAAGATAAAGGCTTCATAAGTGAAACTGTGTTCACTTGTGTATTTCTTTCATTTTTCCTGTGGACTTTCCATCCTTTCATCGTGAAAAGCAAGAAGATATACACTGTGTTGATATGGTGCAGGGTTCTCGCCTTCTTAGTTGCTTGTCAGTTTCTCCGTGTTATAACATTCTATTCAACTCAGCTTCCTGGCCCTAACTATCACTGTCGAGAGGGTTCTGAGCTTGCCAGGTTGCCAAGGCCACATAACGTTCTTGAGGTTCTCTTGCTCAACTTTCCTCGTGGTGTGATATACGGATGTGGAGACCTGATTTTCTCATCGCACATGATATTCACACTAGTCTTTGTCCGCACTTACCAGAAATACGGTTCTAAAAGGTTCATAAAGCTGTTAGGTTGGGTCATTGCCATCTTGCAAAGCCTCTTGATTATTGCGTCCCGTAAACATTACACTGTTGATGTGGTTGTTGCGTGGTATACTGTGAACTTGGTTGTCTTCTTCCTCGACAAGAAATTACCAGAATTGCCTGATCGAACAACGGCATTGCTCCCTGTGATCTCAAAAGACAGAACCAAAGAAGAGAGTCACAAACTCTTGAATGGGAACGGTGTTGATCCTGCAGATCGGAGACCGAGGGCTCAAGTGAATGGCAAAGACAGCAATGGAGGTCACACTGATAATGCTACTAATGGCACATGA

Protein Analysis

305

Amino Acids

34.86

Weight (kDa)

9.49

Isoelectric Point (pI)

35.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAP2_C PF14360 172 - 240 6.9e-24 PAP2 superfamily C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012781)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G37940 AT2G37940 AT3G54020
fragaria_vesca FvH4_6g12480 FvH4_6g12480
malus_domestica MD04G1141900.v1.1 MD12G1156500.v1.1
prunus_persica Prupe.6G267300_v2.0.a1
pyrus_communis pycom04g12890 pycom12g14860
rosa_chinensis RchiOBHm_Chr3g0464301
rosa_laevigata RLG00000024710
rosa_roxburghii Rroxscaffold_6G00415830
rosa_rugosa Rorug03G0071300
rosa_samantha Rh3BG133300 Rh3CG135600 Rh3DG134800
rosa_wichuraiana Rw3G010920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 383
AccB7I CCANNNNNTGG 1 cut(s) 36
AccI GTMKAC 1 cut(s) 695
AccII CGCG 1 cut(s) 21
AccIII TCCGGA 1 cut(s) 208
AciI CCGC 2 cut(s) 19, 568
AclI AACGTT 1 cut(s) 468
AclWI GGATC 1 cut(s) 827
AcoI YGGCCR 1 cut(s) 167
AfiI CCNNNNNNNGG 2 cut(s) 36, 215
AgsI TTSAA 3 cut(s) 185, 396, 817
AhlI ACTAGT 1 cut(s) 556
AjnI CCWGG 4 cut(s) 143, 169, 406, 446
AloI GAACNNNNNNTCC 2 cut(s) 34, 66
AluBI AGCT 7 cut(s) 26, 34, 140, 214, 403, 442, 607
AluI AGCT 7 cut(s) 26, 34, 140, 214, 403, 442, 607
Alw26I GTCTC 3 cut(s) 80, 516, 841
AlwI GGATC 1 cut(s) 827
Aor13HI TCCGGA 1 cut(s) 208
AoxI GGCC 3 cut(s) 167, 409, 458
ApeKI GCWGC 1 cut(s) 140
AspS9I GGNCC 2 cut(s) 173, 410
AvaII GGWCC 1 cut(s) 173
BanII GRGCYC 1 cut(s) 859
BauI CACGAG 1 cut(s) 498
BbsI GAAGAC 2 cut(s) 104, 706
BbvI GCAGC 1 cut(s) 127
BccI CCATC 2 cut(s) 297, 635
BceAI ACGGC 1 cut(s) 774
BcgI CGANNNNNNTGC 2 cut(s) 328, 362
BciT130I CCWGG 4 cut(s) 145, 171, 408, 448
BcoDI GTCTC 3 cut(s) 80, 516, 841
BcuI ACTAGT 1 cut(s) 556
BfaI CTAG 1 cut(s) 557
BfmI CTRYAG 1 cut(s) 837
BisI GCNGC 2 cut(s) 19, 141
BlsI GCNGC 2 cut(s) 20, 142
Bme1390I CCNGG 4 cut(s) 145, 171, 408, 448
Bme18I GGWCC 1 cut(s) 173
BmgT120I GGNCC 2 cut(s) 173, 410
BmrFI CCNGG 4 cut(s) 145, 171, 408, 448
BpiI GAAGAC 2 cut(s) 104, 706
BpuEI CTTGAG 2 cut(s) 494, 843
BsaI GGTCTC 3 cut(s) 80, 516, 841
BsaJI CCNNGG 3 cut(s) 144, 455, 851
BsaWI WCCGGW 1 cut(s) 208
BsaXI ACNNNNNCTCC 2 cut(s) 34, 64
Bsc4I CCNNNNNNNGG 2 cut(s) 36, 215
Bse1I ACTGG 1 cut(s) 95
Bse3DI GCAATG 3 cut(s) 621, 761, 886
BseAI TCCGGA 1 cut(s) 208
BseBI CCWGG 4 cut(s) 145, 171, 408, 448
BseDI CCNNGG 3 cut(s) 144, 455, 851
BseGI GGATG 2 cut(s) 289, 521
BseLI CCNNNNNNNGG 2 cut(s) 36, 215
BseMI GCAATG 3 cut(s) 621, 761, 886
BseMII CTCAG 3 cut(s) 75, 413, 429
BseNI ACTGG 1 cut(s) 95
BseRI GAGGAG 2 cut(s) 55, 68
BseXI GCAGC 1 cut(s) 127
BsgI GTGCAG 1 cut(s) 358
Bsh1236I CGCG 1 cut(s) 21
BshFI GGCC 3 cut(s) 169, 411, 460
BsiSI CCGG 1 cut(s) 209
BslFI GGGAC 1 cut(s) 640
BslI CCNNNNNNNGG 2 cut(s) 36, 215
BsmAI GTCTC 3 cut(s) 80, 516, 841
BsmFI GGGAC 1 cut(s) 640
BsnI GGCC 3 cut(s) 169, 411, 460
Bso31I GGTCTC 3 cut(s) 80, 516, 841
Bsp1286I GDGCHC 1 cut(s) 859
Bsp13I TCCGGA 1 cut(s) 208
Bsp143I GATC 4 cut(s) 748, 774, 832, 841
BspACI CCGC 2 cut(s) 19, 568
BspANI GGCC 3 cut(s) 169, 411, 460
BspCNI CTCAG 3 cut(s) 76, 412, 430
BspEI TCCGGA 1 cut(s) 208
BspFNI CGCG 1 cut(s) 21
BspMAI CTGCAG 1 cut(s) 841
BspPI GGATC 1 cut(s) 827
BspTNI GGTCTC 3 cut(s) 80, 516, 841
BsrDI GCAATG 3 cut(s) 621, 761, 886
BsrI ACTGG 1 cut(s) 95
BssECI CCNNGG 3 cut(s) 144, 455, 851
BssMI GATC 4 cut(s) 748, 774, 832, 841
BssNAI GTATAC 1 cut(s) 696
BssSI CACGAG 1 cut(s) 498
BssT1I CCWWGG 1 cut(s) 455
Bst1107I GTATAC 1 cut(s) 696
Bst2BI CACGAG 1 cut(s) 498
Bst2UI CCWGG 4 cut(s) 145, 171, 408, 448
Bst4CI ACNGT 7 cut(s) 247, 325, 425, 587, 673, 700, 827
Bst6I CTCTTC 1 cut(s) 792
BstC8I GCNNGC 1 cut(s) 444
BstDEI CTNAG 4 cut(s) 84, 355, 399, 438
BstF5I GGATG 2 cut(s) 289, 521
BstFNI CGCG 1 cut(s) 21
BstKTI GATC 4 cut(s) 751, 777, 835, 844
BstMAI GTCTC 3 cut(s) 80, 516, 841
BstMBI GATC 4 cut(s) 748, 774, 832, 841
BstMWI GCNNNNNNNGC 1 cut(s) 876
BstNI CCWGG 4 cut(s) 145, 171, 408, 448
BstSCI CCNGG 4 cut(s) 143, 169, 406, 446
BstSFI CTRYAG 1 cut(s) 837
BstUI CGCG 1 cut(s) 21
BstV1I GCAGC 1 cut(s) 127
BstV2I GAAGAC 2 cut(s) 104, 706
BstZ17I GTATAC 1 cut(s) 696
BsuRI GGCC 3 cut(s) 169, 411, 460
BtgZI GCGATG 1 cut(s) 522
BtsCI GGATG 2 cut(s) 289, 521
BtsIMutI CAGTG 4 cut(s) 321, 421, 669, 891
Cac8I GCNNGC 1 cut(s) 444
Cfr13I GGNCC 2 cut(s) 173, 410
CseI GACGC 2 cut(s) 13, 642
CviAII CATG 3 cut(s) 131, 544, 915
DdeI CTNAG 4 cut(s) 84, 355, 399, 438
DpnI GATC 4 cut(s) 750, 776, 834, 843
DpnII GATC 4 cut(s) 748, 774, 832, 841
EaeI YGGCCR 1 cut(s) 167
Eam1104I CTCTTC 1 cut(s) 792
EarI CTCTTC 1 cut(s) 792
Eco130I CCWWGG 1 cut(s) 455
Eco24I GRGCYC 1 cut(s) 859
Eco31I GGTCTC 3 cut(s) 80, 516, 841
Eco47I GGWCC 1 cut(s) 173
EcoRII CCWGG 4 cut(s) 143, 169, 406, 446
EcoT14I CCWWGG 1 cut(s) 455
EcoT38I GRGCYC 1 cut(s) 859
ErhI CCWWGG 1 cut(s) 455
FaeI CATG 3 cut(s) 134, 547, 918
FaqI GGGAC 1 cut(s) 640
FatI CATG 3 cut(s) 130, 543, 914
FblI GTMKAC 1 cut(s) 695
Fnu4HI GCNGC 2 cut(s) 19, 141
FokI GGATG 2 cut(s) 276, 528
FriOI GRGCYC 1 cut(s) 859
Fsp4HI GCNGC 2 cut(s) 19, 141
FspBI CTAG 1 cut(s) 557
GluI GCNGC 2 cut(s) 19, 141
HaeIII GGCC 3 cut(s) 169, 411, 460
HapII CCGG 1 cut(s) 209
HgaI GACGC 2 cut(s) 13, 642
Hin1II CATG 3 cut(s) 134, 547, 918
HindIII AAGCTT 1 cut(s) 24
HinfI GANTC 2 cut(s) 188, 802
HpaII CCGG 1 cut(s) 209
Hpy166II GTNNAC 5 cut(s) 252, 282, 662, 696, 703
Hpy188I TCNGA 3 cut(s) 55, 439, 846
Hpy188III TCNNGA 8 cut(s) 185, 209, 221, 301, 428, 473, 643, 814
Hpy8I GTNNAC 5 cut(s) 252, 282, 662, 696, 703
HpyAV CCTTC 2 cut(s) 110, 361
HpyCH4III ACNGT 7 cut(s) 247, 325, 425, 587, 673, 700, 827
HpyCH4IV ACGT 1 cut(s) 468
HpyCH4V TGCA 3 cut(s) 339, 634, 839
HpyF10VI GCNNNNNNNGC 1 cut(s) 876
HpyF3I CTNAG 4 cut(s) 84, 355, 399, 438
HpySE526I ACGT 1 cut(s) 468
Hsp92II CATG 3 cut(s) 134, 547, 918
Kpn2I TCCGGA 1 cut(s) 208
Kzo9I GATC 4 cut(s) 748, 774, 832, 841
LmnI GCTCC 2 cut(s) 211, 771
Lsp1109I GCAGC 1 cut(s) 127
MaeI CTAG 1 cut(s) 557
MaeII ACGT 1 cut(s) 468
MaeIII GTNAC 2 cut(s) 803, 887
MalI GATC 4 cut(s) 750, 776, 834, 843
MboI GATC 4 cut(s) 748, 774, 832, 841
MboII GAAGA 6 cut(s) 104, 197, 325, 706, 709, 809
MhlI GDGCHC 1 cut(s) 859
MluCI AATT 2 cut(s) 731, 740
MlyI GAGTC 1 cut(s) 811
MnlI CCTC 9 cut(s) 33, 89, 424, 469, 507, 650, 731, 846, 878
MroI TCCGGA 1 cut(s) 208
MspI CCGG 1 cut(s) 209
MspR9I CCNGG 4 cut(s) 145, 171, 408, 448
MvaI CCWGG 4 cut(s) 145, 171, 408, 448
MvnI CGCG 1 cut(s) 21
MwoI GCNNNNNNNGC 1 cut(s) 876
NdeII GATC 4 cut(s) 748, 774, 832, 841
NlaIII CATG 3 cut(s) 134, 547, 918
NmuCI GTSAC 2 cut(s) 803, 887
PfeI GAWTC 1 cut(s) 188
PflMI CCANNNNNTGG 1 cut(s) 36
PkrI GCNGC 2 cut(s) 20, 142
PleI GAGTC 1 cut(s) 810
PpsI GAGTC 1 cut(s) 810
PsiI TTATAA 1 cut(s) 383
Psp1406I AACGTT 1 cut(s) 468
Psp6I CCWGG 4 cut(s) 143, 169, 406, 446
PspGI CCWGG 4 cut(s) 143, 169, 406, 446
PspPI GGNCC 2 cut(s) 173, 410
PsrI GAACNNNNNNTAC 2 cut(s) 80, 112
PstI CTGCAG 1 cut(s) 841
SatI GCNGC 2 cut(s) 19, 141
Sau3AI GATC 4 cut(s) 748, 774, 832, 841
Sau96I GGNCC 2 cut(s) 173, 410
SchI GAGTC 1 cut(s) 811
ScrFI CCNGG 4 cut(s) 145, 171, 408, 448
SduI GDGCHC 1 cut(s) 859
SfcI CTRYAG 1 cut(s) 837
SinI GGWCC 1 cut(s) 173
SmlI CTYRAG 2 cut(s) 473, 858
SmoI CTYRAG 2 cut(s) 473, 858
SpeI ACTAGT 1 cut(s) 556
Sse9I AATT 2 cut(s) 731, 740
SsiI CCGC 2 cut(s) 19, 568
SspMI CTAG 1 cut(s) 557
StyD4I CCNGG 4 cut(s) 143, 169, 406, 446
StyI CCWWGG 1 cut(s) 455
TaaI ACNGT 7 cut(s) 247, 325, 425, 587, 673, 700, 827
TaiI ACGT 1 cut(s) 471
TaqI TCGA 3 cut(s) 427, 723, 751
TaqII GACCGA 1 cut(s) 865
TasI AATT 2 cut(s) 731, 740
TauI GCSGC 1 cut(s) 21
TfiI GAWTC 1 cut(s) 188
TscAI CASTG 4 cut(s) 328, 428, 676, 898
TseFI GTSAC 2 cut(s) 803, 887
TseI GCWGC 1 cut(s) 140
Tsp45I GTSAC 2 cut(s) 803, 887
TspDTI ATGAA 7 cut(s) 119, 143, 152, 223, 258, 286, 589
TspGWI ACGGA 3 cut(s) 80, 365, 528
TspRI CASTG 4 cut(s) 328, 428, 676, 898
Van91I CCANNNNNTGG 1 cut(s) 36
VpaK11BI GGWCC 1 cut(s) 173
XmiI GTMKAC 1 cut(s) 695
XspI CTAG 1 cut(s) 557
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.