MD12G1156500.v1.1

phosphatidylinositol ceramide inositolphosphotransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0012781
Physical Location & Seq
Forward (+)
23708856 .. 23713295
4440 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1156500.v1.1.491

Sequence Viewer

Length: 945 bp
ATGACGCTTTACATTGGCCGCGAGTCGTCAAAGCTCTGGAAGAGAATCTGCGCTGAGGTAGTCACCGAGATCAATCTCCTCGCCACGAATTGGAAGTATCTTCTCGCCGGTCTAGTTTTTCAGTACATACATGGTCTAGCTGCTCGAGGAGTTCATTATATACATCGGCCCGGGCCAACGCTCCAGGATGCTGGTTTCTTTCTTCTTCCAGAACTTGGGCAAGACAGAGCCTACATCAGTGAGACCCTTTTCACCTTCATCTTCTTATCTTTTGTATTGTGGACCTTCCATCCTTTCATTTTCAAGGGCAAAAAGATTTACACAGTTCTCCTCTGGTGTAGGGTTCTAGCATTCTTATCGGCTTGTCAATTTCTGCGGATTATCACATTCTATTCCACCCAGCTTCCTGGTCCAAATTACCATTGTCGAGAGGGTTCTAAACTTGCCAGGCTGCCTCCTCCGGAAAGTGTATTAGAAGTCTTCTTGATCATTCCGCATGGTGTACTTTATGGTTGTGGTGATTTGATTTTCTCATCGCATATGATATTTAGTCTAGTCTTTGTGCGCACTTATCAGAAATACGGTACACAAAGGTTTATAAAGCAGTTAGCTTGGTTACTCGTTGTGATCCAGAGTTTCTTGATTGTGGCATCGCGCAAACATTACACGGTTGATGTTGTTGTTGCATGGTATACTGTTAATTTGGTCGTATTCTTTATCGACAAGAAATTGGCAGAATTACCTGACCGGACCAACGGAGCTGCATCTCTCTTGTTACCGCTAAGCACAAAGGACAAGGATGGTAAGACCAAAGAAGAGAACCACAAGCTATTGAATGGAATTGTCGGAGACCCTGCTGATCGGAGACAAAGAACTCAAGTTAACGGCAAGACTCTAGATGATGTAAACACAGTACATGCCGATGCTACAACGAACGCTGCATAG

Protein Analysis

315

Amino Acids

35.73

Weight (kDa)

9.34

Isoelectric Point (pI)

26.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAP2_C PF14360 171 - 239 1.6e-23 PAP2 superfamily C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012781)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G37940 AT2G37940 AT3G54020
fragaria_vesca FvH4_6g12480 FvH4_6g12480
malus_domestica MD04G1141900.v1.1 MD12G1156500.v1.1
prunus_persica Prupe.6G267300_v2.0.a1
pyrus_communis pycom04g12890 pycom12g14860
rosa_chinensis RchiOBHm_Chr3g0464301
rosa_laevigata RLG00000024710
rosa_roxburghii Rroxscaffold_6G00415830
rosa_rugosa Rorug03G0071300
rosa_samantha Rh3BG133300 Rh3CG135600 Rh3DG134800
rosa_wichuraiana Rw3G010920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 599
Acc16I TGCGCA 1 cut(s) 566
AccB7I CCANNNNNTGG 2 cut(s) 90, 215
AccI GTMKAC 1 cut(s) 692
AccII CGCG 2 cut(s) 21, 655
AccIII TCCGGA 1 cut(s) 460
AciI CCGC 4 cut(s) 19, 376, 494, 779
AclWI GGATC 1 cut(s) 622
AcoI YGGCCR 1 cut(s) 16
AfaI GTAC 4 cut(s) 125, 504, 586, 915
AfiI CCNNNNNNNGG 2 cut(s) 90, 215
AgsI TTSAA 2 cut(s) 304, 835
AjnI CCWGG 3 cut(s) 183, 406, 446
AluBI AGCT 6 cut(s) 34, 140, 403, 611, 761, 829
AluI AGCT 6 cut(s) 34, 140, 403, 611, 761, 829
Alw26I GTCTC 3 cut(s) 236, 843, 859
AlwI GGATC 1 cut(s) 622
Ama87I CYCGRG 2 cut(s) 144, 170
Aor13HI TCCGGA 1 cut(s) 460
AoxI GGCC 3 cut(s) 16, 167, 173
ApeKI GCWGC 4 cut(s) 140, 451, 761, 938
AspLEI GCGC 3 cut(s) 53, 567, 657
AspS9I GGNCC 5 cut(s) 168, 173, 282, 410, 750
AsuC2I CCSGG 2 cut(s) 171, 172
AsuHPI GGTGA 3 cut(s) 55, 244, 530
AvaI CYCGRG 2 cut(s) 144, 170
AvaII GGWCC 3 cut(s) 282, 410, 750
BbsI GAAGAC 1 cut(s) 472
BbvCI CCTCAGC 1 cut(s) 54
BbvI GCAGC 4 cut(s) 127, 438, 748, 925
BccI CCATC 2 cut(s) 297, 794
BceAI ACGGC 1 cut(s) 901
BcgI CGANNNNNNTGC 2 cut(s) 339, 373
BciT130I CCWGG 3 cut(s) 185, 408, 448
BclI TGATCA 1 cut(s) 486
BcnI CCSGG 2 cut(s) 171, 172
BcoDI GTCTC 3 cut(s) 236, 843, 859
BfaI CTAG 5 cut(s) 113, 137, 347, 554, 896
BisI GCNGC 5 cut(s) 19, 141, 452, 762, 939
BlpI GCTNAGC 1 cut(s) 782
BlsI GCNGC 5 cut(s) 20, 142, 453, 763, 940
Bme1390I CCNGG 5 cut(s) 171, 172, 185, 408, 448
Bme18I GGWCC 3 cut(s) 282, 410, 750
BmeT110I CYCGRG 2 cut(s) 144, 170
BmgT120I GGNCC 5 cut(s) 168, 173, 282, 410, 750
BmrFI CCNGG 5 cut(s) 171, 172, 185, 408, 448
BmsI GCATC 4 cut(s) 178, 659, 773, 913
BpiI GAAGAC 1 cut(s) 472
BpmI CTGGAG 1 cut(s) 167
Bpu10I CCTNAGC 1 cut(s) 54
Bpu1102I GCTNAGC 1 cut(s) 782
BpuEI CTTGAG 1 cut(s) 861
BpuMI CCSGG 2 cut(s) 171, 172
BsaI GGTCTC 2 cut(s) 236, 843
BsaJI CCNNGG 1 cut(s) 170
BsaWI WCCGGW 2 cut(s) 460, 747
Bsc4I CCNNNNNNNGG 2 cut(s) 90, 215
Bse118I RCCGGY 1 cut(s) 107
BseAI TCCGGA 1 cut(s) 460
BseBI CCWGG 3 cut(s) 185, 408, 448
BseDI CCNNGG 1 cut(s) 170
BseGI GGATG 3 cut(s) 193, 289, 805
BseLI CCNNNNNNNGG 2 cut(s) 90, 215
BseMII CTCAG 1 cut(s) 45
BseRI GAGGAG 4 cut(s) 68, 162, 320, 447
BseXI GCAGC 4 cut(s) 127, 438, 748, 925
BseYI CCCAGC 1 cut(s) 399
Bsh1236I CGCG 2 cut(s) 21, 655
BshFI GGCC 3 cut(s) 18, 169, 175
BsiHKCI CYCGRG 2 cut(s) 144, 170
BsiSI CCGG 4 cut(s) 108, 171, 461, 748
BslI CCNNNNNNNGG 2 cut(s) 90, 215
BsmAI GTCTC 3 cut(s) 236, 843, 859
BsmI GAATGC 1 cut(s) 350
BsnI GGCC 3 cut(s) 18, 169, 175
Bso31I GGTCTC 2 cut(s) 236, 843
BsoBI CYCGRG 2 cut(s) 144, 170
Bsp13I TCCGGA 1 cut(s) 460
Bsp143I GATC 4 cut(s) 69, 486, 627, 859
Bsp1720I GCTNAGC 1 cut(s) 782
BspACI CCGC 4 cut(s) 19, 376, 494, 779
BspANI GGCC 3 cut(s) 18, 169, 175
BspCNI CTCAG 1 cut(s) 46
BspEI TCCGGA 1 cut(s) 460
BspFNI CGCG 2 cut(s) 21, 655
BspPI GGATC 1 cut(s) 622
BspTNI GGTCTC 2 cut(s) 236, 843
BsrFI RCCGGY 1 cut(s) 107
BssAI RCCGGY 1 cut(s) 107
BssECI CCNNGG 1 cut(s) 170
BssMI GATC 4 cut(s) 69, 486, 627, 859
BssNAI GTATAC 1 cut(s) 693
Bst1107I GTATAC 1 cut(s) 693
Bst2UI CCWGG 3 cut(s) 185, 408, 448
Bst4CI ACNGT 5 cut(s) 325, 584, 670, 697, 913
Bst6I CTCTTC 2 cut(s) 35, 810
BstDEI CTNAG 2 cut(s) 54, 782
BstF5I GGATG 3 cut(s) 193, 289, 805
BstFNI CGCG 2 cut(s) 21, 655
BstHHI GCGC 3 cut(s) 53, 567, 657
BstKTI GATC 4 cut(s) 72, 489, 630, 862
BstMAI GTCTC 3 cut(s) 236, 843, 859
BstMBI GATC 4 cut(s) 69, 486, 627, 859
BstNI CCWGG 3 cut(s) 185, 408, 448
BstNSI RCATGY 1 cut(s) 920
BstSCI CCNGG 5 cut(s) 169, 170, 183, 406, 446
BstUI CGCG 2 cut(s) 21, 655
BstV1I GCAGC 4 cut(s) 127, 438, 748, 925
BstV2I GAAGAC 1 cut(s) 472
BstXI CCANNNNNNTGG 2 cut(s) 191, 407
BstZ17I GTATAC 1 cut(s) 693
BsuRI GGCC 3 cut(s) 18, 169, 175
BtgZI GCGATG 2 cut(s) 519, 636
BtsCI GGATG 3 cut(s) 193, 289, 805
BtsIMutI CAGTG 1 cut(s) 244
CfoI GCGC 3 cut(s) 53, 567, 657
Cfr10I RCCGGY 1 cut(s) 107
Cfr13I GGNCC 5 cut(s) 168, 173, 282, 410, 750
Cfr9I CCCGGG 1 cut(s) 170
CseI GACGC 1 cut(s) 13
Csp6I GTAC 4 cut(s) 124, 503, 585, 914
CviAII CATG 4 cut(s) 131, 497, 687, 917
CviQI GTAC 4 cut(s) 124, 503, 585, 914
DdeI CTNAG 2 cut(s) 54, 782
DpnI GATC 4 cut(s) 71, 488, 629, 861
DpnII GATC 4 cut(s) 69, 486, 627, 859
EaeI YGGCCR 1 cut(s) 16
Eam1104I CTCTTC 2 cut(s) 35, 810
EarI CTCTTC 2 cut(s) 35, 810
Eco31I GGTCTC 2 cut(s) 236, 843
Eco47I GGWCC 3 cut(s) 282, 410, 750
Eco88I CYCGRG 2 cut(s) 144, 170
EcoRII CCWGG 3 cut(s) 183, 406, 446
FaeI CATG 4 cut(s) 134, 500, 690, 920
FatI CATG 4 cut(s) 130, 496, 686, 916
FauNDI CATATG 1 cut(s) 540
FbaI TGATCA 1 cut(s) 486
FblI GTMKAC 1 cut(s) 692
Fnu4HI GCNGC 5 cut(s) 19, 141, 452, 762, 939
FokI GGATG 3 cut(s) 200, 276, 812
Fsp4HI GCNGC 5 cut(s) 19, 141, 452, 762, 939
FspAI RTGCGCAY 1 cut(s) 566
FspBI CTAG 5 cut(s) 113, 137, 347, 554, 896
FspI TGCGCA 1 cut(s) 566
GlaI GCGC 3 cut(s) 52, 566, 656
GluI GCNGC 5 cut(s) 19, 141, 452, 762, 939
GsaI CCCAGC 1 cut(s) 403
GsuI CTGGAG 1 cut(s) 167
HaeIII GGCC 3 cut(s) 18, 169, 175
HapII CCGG 4 cut(s) 108, 171, 461, 748
HgaI GACGC 1 cut(s) 13
HhaI GCGC 3 cut(s) 53, 567, 657
Hin1II CATG 4 cut(s) 134, 500, 690, 920
Hin6I GCGC 3 cut(s) 51, 565, 655
HinP1I GCGC 3 cut(s) 51, 565, 655
HincII GTYRAC 1 cut(s) 883
HindII GTYRAC 1 cut(s) 883
HinfI GANTC 3 cut(s) 23, 45, 892
HpaI GTTAAC 1 cut(s) 883
HpaII CCGG 4 cut(s) 108, 171, 461, 748
HphI GGTGA 3 cut(s) 55, 244, 530
Hpy166II GTNNAC 6 cut(s) 282, 503, 587, 693, 883, 907
Hpy188I TCNGA 3 cut(s) 576, 848, 864
Hpy188III TCNNGA 8 cut(s) 37, 209, 428, 461, 484, 631, 640, 896
Hpy8I GTNNAC 6 cut(s) 282, 503, 587, 693, 883, 907
HpyAV CCTTC 2 cut(s) 265, 295
HpyCH4III ACNGT 5 cut(s) 325, 584, 670, 697, 913
HpyCH4V TGCA 3 cut(s) 686, 764, 941
HpyF3I CTNAG 2 cut(s) 54, 782
Hsp92II CATG 4 cut(s) 134, 500, 690, 920
HspAI GCGC 3 cut(s) 51, 565, 655
Kpn2I TCCGGA 1 cut(s) 460
Ksp22I TGATCA 1 cut(s) 486
KspAI GTTAAC 1 cut(s) 883
Kzo9I GATC 4 cut(s) 69, 486, 627, 859
LmnI GCTCC 2 cut(s) 186, 758
Lsp1109I GCAGC 4 cut(s) 127, 438, 748, 925
LweI GCATC 4 cut(s) 178, 659, 773, 913
MaeI CTAG 5 cut(s) 113, 137, 347, 554, 896
MaeIII GTNAC 3 cut(s) 61, 615, 774
MalI GATC 4 cut(s) 71, 488, 629, 861
MboI GATC 4 cut(s) 69, 486, 627, 859
MboII GAAGA 7 cut(s) 52, 92, 194, 197, 253, 472, 827
MluCI AATT 7 cut(s) 88, 368, 415, 700, 728, 737, 840
MlyI GAGTC 2 cut(s) 32, 886
MmeI TCCRAC 1 cut(s) 826
MnlI CCTC 7 cut(s) 49, 89, 140, 341, 424, 465, 468
MroI TCCGGA 1 cut(s) 460
MseI TTAA 2 cut(s) 699, 882
MslI CAYNNNNRTG 1 cut(s) 921
MspI CCGG 4 cut(s) 108, 171, 461, 748
MspR9I CCNGG 5 cut(s) 171, 172, 185, 408, 448
Mva1269I GAATGC 1 cut(s) 350
MvaI CCWGG 3 cut(s) 185, 408, 448
MvnI CGCG 2 cut(s) 21, 655
NciI CCSGG 2 cut(s) 171, 172
NdeI CATATG 1 cut(s) 540
NdeII GATC 4 cut(s) 69, 486, 627, 859
NlaIII CATG 4 cut(s) 134, 500, 690, 920
NmuCI GTSAC 1 cut(s) 61
NsbI TGCGCA 1 cut(s) 566
NspI RCATGY 1 cut(s) 920
PaeR7I CTCGAG 1 cut(s) 144
PctI GAATGC 1 cut(s) 350
PfeI GAWTC 1 cut(s) 45
PflMI CCANNNNNTGG 2 cut(s) 90, 215
PfoI TCCNGGA 1 cut(s) 183
PkrI GCNGC 5 cut(s) 20, 142, 453, 763, 940
PleI GAGTC 2 cut(s) 31, 886
PpsI GAGTC 2 cut(s) 31, 886
PsiI TTATAA 1 cut(s) 599
Psp6I CCWGG 3 cut(s) 183, 406, 446
PspFI CCCAGC 1 cut(s) 399
PspGI CCWGG 3 cut(s) 183, 406, 446
PspPI GGNCC 5 cut(s) 168, 173, 282, 410, 750
PspXI VCTCGAGB 1 cut(s) 144
RsaI GTAC 4 cut(s) 125, 504, 586, 915
RsaNI GTAC 4 cut(s) 124, 503, 585, 914
RseI CAYNNNNRTG 1 cut(s) 921
SaqAI TTAA 2 cut(s) 699, 882
SatI GCNGC 5 cut(s) 19, 141, 452, 762, 939
Sau3AI GATC 4 cut(s) 69, 486, 627, 859
Sau96I GGNCC 5 cut(s) 168, 173, 282, 410, 750
SchI GAGTC 2 cut(s) 32, 886
ScrFI CCNGG 5 cut(s) 171, 172, 185, 408, 448
SfaNI GCATC 4 cut(s) 178, 659, 773, 913
Sfr274I CTCGAG 1 cut(s) 144
SinI GGWCC 3 cut(s) 282, 410, 750
SlaI CTCGAG 1 cut(s) 144
SmaI CCCGGG 1 cut(s) 172
SmiMI CAYNNNNRTG 1 cut(s) 921
SmlI CTYRAG 2 cut(s) 144, 876
SmoI CTYRAG 2 cut(s) 144, 876
SrfI GCCCGGGC 1 cut(s) 172
Sse9I AATT 7 cut(s) 88, 368, 415, 700, 728, 737, 840
SsiI CCGC 4 cut(s) 19, 376, 494, 779
SspMI CTAG 5 cut(s) 113, 137, 347, 554, 896
StyD4I CCNGG 5 cut(s) 169, 170, 183, 406, 446
TaaI ACNGT 5 cut(s) 325, 584, 670, 697, 913
TaqI TCGA 3 cut(s) 145, 427, 720
TasI AATT 7 cut(s) 88, 368, 415, 700, 728, 737, 840
TatI WGTACW 3 cut(s) 123, 502, 913
TauI GCSGC 1 cut(s) 21
TfiI GAWTC 1 cut(s) 45
Tru1I TTAA 2 cut(s) 699, 882
Tru9I TTAA 2 cut(s) 699, 882
TscAI CASTG 1 cut(s) 244
TseFI GTSAC 1 cut(s) 61
TseI GCWGC 4 cut(s) 140, 451, 761, 938
Tsp45I GTSAC 1 cut(s) 61
TspDTI ATGAA 3 cut(s) 143, 247, 286
TspGWI ACGGA 1 cut(s) 771
TspMI CCCGGG 1 cut(s) 170
TspRI CASTG 1 cut(s) 244
Van91I CCANNNNNTGG 2 cut(s) 90, 215
VpaK11BI GGWCC 3 cut(s) 282, 410, 750
XbaI TCTAGA 1 cut(s) 895
XceI RCATGY 1 cut(s) 920
XhoI CTCGAG 1 cut(s) 144
XmaI CCCGGG 1 cut(s) 170
XmiI GTMKAC 1 cut(s) 692
XspI CTAG 5 cut(s) 113, 137, 347, 554, 896
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.