RLG00000024710

phosphatidylinositol ceramide inositolphosphotransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0012781
Physical Location & Seq
Forward (+)
38432697 .. 38435979
3283 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024710

Sequence Viewer

Length: 942 bp
ATGACGCTTTACATTGGTCGCGAGTCGTCCAAGCTTTGGAAGAGGATTTGCGCCGAGGTAGTCACCGAGATCAATCTCCTCGCCGCGAATTGGAAGTATCTTCTTGCCGGCCTTGTTGGTCAGTACATACATGGTCTAGCAGCTCGAGGAGTTCATTATATACATCGTCCAGGGCCAACACTGCAGGATGCTGGTTTCTTTCTTCTTCCGGAGCTTGGGGAAGAGAGAGCTTACATCAGTGAAACCCTGTTCACCTTCATCTTTTTATCCTTTGTCTTGTGGACTTTTCATCCTTTCATTTTCAAGAGCAAAAAGATATACACAGTTCTAATATGGTGTAGGGTTCTAGCATTTTTAGTGACTTGTCAGTTTCTTCGAATCCTCACATTCTACTCCACCCAGCTTCCTGGTCCAAATTACCATTGTCGTGAGGGCTCTAGACTTGCTAGGTTGCCTCCTCCAGAGACTGTATTTGAGGCCCTTGTGATCGTTCCGAGGGGTGTACTATATGGCTGTGGCGATTTGATTTTTTCGTCACACATGATCTTTAGTCTCACCTTTGTGCGCACTTATCAGAAATATGGCACACAAAGATTTATAAAGCAGTTGGCTTGGATGCTCGTTGTGATCCAGAGTTTGTTGATTGTGGCATCCCGCAAACATTACACAGTTGATGTTGTTGTTGCATGGTACACTGTTAATTTGGTTGTATTCTTTATCGACAAGAAATTAGCAGAATTACCGGACCGGACAGCTACATCATTGTTGTTACCACTAAGCACGAAGGACAAGGACAGCAAGACTAAAGAAGAGAATCACAAGCTATTGAATGGAATGTCTGGAGACCCCGCTGATCGGAGGCAAAGAACTCAAGTTAATGGCAAGATTCTAGAAGACGGAAACACAGTAGTGCATGCCGATGCTACAATGAACGGTGCATAG

Protein Analysis

314

Amino Acids

35.68

Weight (kDa)

9.35

Isoelectric Point (pI)

32.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAP2_C PF14360 171 - 239 9e-23 PAP2 superfamily C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012781)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G37940 AT2G37940 AT3G54020
fragaria_vesca FvH4_6g12480 FvH4_6g12480
malus_domestica MD04G1141900.v1.1 MD12G1156500.v1.1
prunus_persica Prupe.6G267300_v2.0.a1
pyrus_communis pycom04g12890 pycom12g14860
rosa_chinensis RchiOBHm_Chr3g0464301
rosa_laevigata RLG00000024710
rosa_roxburghii Rroxscaffold_6G00415830
rosa_rugosa Rorug03G0071300
rosa_samantha Rh3BG133300 Rh3CG135600 Rh3DG134800
rosa_wichuraiana Rw3G010920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 599
Acc16I TGCGCA 1 cut(s) 566
AccB7I CCANNNNNTGG 1 cut(s) 36
AccII CGCG 2 cut(s) 21, 86
AccIII TCCGGA 1 cut(s) 208
AciI CCGC 3 cut(s) 84, 655, 849
AclWI GGATC 1 cut(s) 622
AfaI GTAC 3 cut(s) 125, 504, 692
AfiI CCNNNNNNNGG 4 cut(s) 36, 90, 215, 855
AgsI TTSAA 2 cut(s) 304, 829
AjnI CCWGG 2 cut(s) 169, 406
AleI CACNNNNGTG 2 cut(s) 560, 908
AluBI AGCT 7 cut(s) 34, 143, 214, 230, 403, 755, 823
AluI AGCT 7 cut(s) 34, 143, 214, 230, 403, 755, 823
Alw26I GTCTC 3 cut(s) 458, 557, 837
AlwI GGATC 1 cut(s) 622
AlwNI CAGNNNCTG 1 cut(s) 467
Ama87I CYCGRG 1 cut(s) 144
Aor13HI TCCGGA 1 cut(s) 208
AoxI GGCC 3 cut(s) 109, 173, 477
ApeKI GCWGC 1 cut(s) 140
AspLEI GCGC 2 cut(s) 53, 567
AspS9I GGNCC 4 cut(s) 173, 410, 478, 745
AsuHPI GGTGA 3 cut(s) 55, 244, 547
AsuII TTCGAA 1 cut(s) 376
AvaI CYCGRG 1 cut(s) 144
AvaII GGWCC 2 cut(s) 410, 745
BanII GRGCYC 1 cut(s) 437
BbsI GAAGAC 1 cut(s) 900
BbvI GCAGC 1 cut(s) 152
BciT130I CCWGG 2 cut(s) 171, 408
BcoDI GTCTC 3 cut(s) 458, 557, 837
BfaI CTAG 5 cut(s) 137, 347, 438, 447, 890
BfmI CTRYAG 1 cut(s) 182
BisI GCNGC 2 cut(s) 84, 141
BlsI GCNGC 2 cut(s) 85, 142
Bme1390I CCNGG 2 cut(s) 171, 408
Bme18I GGWCC 2 cut(s) 410, 745
BmeT110I CYCGRG 1 cut(s) 144
BmgT120I GGNCC 4 cut(s) 173, 410, 478, 745
BmrFI CCNGG 2 cut(s) 171, 408
BmsI GCATC 4 cut(s) 178, 606, 659, 910
BpiI GAAGAC 1 cut(s) 900
BpmI CTGGAG 2 cut(s) 444, 861
Bpu14I TTCGAA 1 cut(s) 376
BpuEI CTTGAG 1 cut(s) 855
BsaI GGTCTC 1 cut(s) 837
BsaJI CCNNGG 3 cut(s) 54, 170, 494
BsaWI WCCGGW 3 cut(s) 208, 742, 747
Bsc4I CCNNNNNNNGG 4 cut(s) 36, 90, 215, 855
Bse118I RCCGGY 1 cut(s) 107
BseAI TCCGGA 1 cut(s) 208
BseBI CCWGG 2 cut(s) 171, 408
BseDI CCNNGG 3 cut(s) 54, 170, 494
BseGI GGATG 4 cut(s) 193, 289, 621, 650
BseLI CCNNNNNNNGG 4 cut(s) 36, 90, 215, 855
BseRI GAGGAG 3 cut(s) 68, 162, 447
BseXI GCAGC 1 cut(s) 152
BseYI CCCAGC 1 cut(s) 399
Bsh1236I CGCG 2 cut(s) 21, 86
BshFI GGCC 3 cut(s) 111, 175, 479
BsiHKCI CYCGRG 1 cut(s) 144
BsiSI CCGG 4 cut(s) 108, 209, 743, 748
BslI CCNNNNNNNGG 4 cut(s) 36, 90, 215, 855
BsmAI GTCTC 3 cut(s) 458, 557, 837
BsnI GGCC 3 cut(s) 111, 175, 479
Bso31I GGTCTC 1 cut(s) 837
BsoBI CYCGRG 1 cut(s) 144
Bsp119I TTCGAA 1 cut(s) 376
Bsp1286I GDGCHC 1 cut(s) 437
Bsp13I TCCGGA 1 cut(s) 208
Bsp143I GATC 5 cut(s) 69, 486, 543, 627, 853
Bsp68I TCGCGA 1 cut(s) 21
BspACI CCGC 3 cut(s) 84, 655, 849
BspANI GGCC 3 cut(s) 111, 175, 479
BspEI TCCGGA 1 cut(s) 208
BspFNI CGCG 2 cut(s) 21, 86
BspMAI CTGCAG 1 cut(s) 186
BspPI GGATC 1 cut(s) 622
BspT104I TTCGAA 1 cut(s) 376
BspTNI GGTCTC 1 cut(s) 837
BsrFI RCCGGY 1 cut(s) 107
BssAI RCCGGY 1 cut(s) 107
BssECI CCNNGG 3 cut(s) 54, 170, 494
BssMI GATC 5 cut(s) 69, 486, 543, 627, 853
Bst2UI CCWGG 2 cut(s) 171, 408
Bst4CI ACNGT 6 cut(s) 325, 469, 670, 697, 907, 935
Bst6I CTCTTC 3 cut(s) 35, 216, 804
BstBI TTCGAA 1 cut(s) 376
BstC8I GCNNGC 2 cut(s) 109, 915
BstDEI CTNAG 1 cut(s) 776
BstF5I GGATG 4 cut(s) 193, 289, 621, 650
BstFNI CGCG 2 cut(s) 21, 86
BstHHI GCGC 2 cut(s) 53, 567
BstKTI GATC 5 cut(s) 72, 489, 546, 630, 856
BstMAI GTCTC 3 cut(s) 458, 557, 837
BstMBI GATC 5 cut(s) 69, 486, 543, 627, 853
BstMWI GCNNNNNNNGC 1 cut(s) 181
BstNI CCWGG 2 cut(s) 171, 408
BstNSI RCATGY 1 cut(s) 917
BstSCI CCNGG 2 cut(s) 169, 406
BstSFI CTRYAG 1 cut(s) 182
BstUI CGCG 2 cut(s) 21, 86
BstV1I GCAGC 1 cut(s) 152
BstV2I GAAGAC 1 cut(s) 900
BstXI CCANNNNNNTGG 1 cut(s) 407
BsuRI GGCC 3 cut(s) 111, 175, 479
BtsCI GGATG 4 cut(s) 193, 289, 621, 650
BtsI GCAGTG 1 cut(s) 179
BtsIMutI CAGTG 3 cut(s) 179, 244, 693
BtuMI TCGCGA 1 cut(s) 21
Cac8I GCNNGC 2 cut(s) 109, 915
CaiI CAGNNNCTG 1 cut(s) 467
CfoI GCGC 2 cut(s) 53, 567
Cfr10I RCCGGY 1 cut(s) 107
Cfr13I GGNCC 4 cut(s) 173, 410, 478, 745
CpoI CGGWCCG 1 cut(s) 745
CseI GACGC 1 cut(s) 13
Csp6I GTAC 3 cut(s) 124, 503, 691
CspI CGGWCCG 1 cut(s) 745
CviAII CATG 4 cut(s) 131, 541, 687, 914
CviQI GTAC 3 cut(s) 124, 503, 691
DdeI CTNAG 1 cut(s) 776
DpnI GATC 5 cut(s) 71, 488, 545, 629, 855
DpnII GATC 5 cut(s) 69, 486, 543, 627, 853
Eam1104I CTCTTC 3 cut(s) 35, 216, 804
EarI CTCTTC 3 cut(s) 35, 216, 804
Eco24I GRGCYC 1 cut(s) 437
Eco31I GGTCTC 1 cut(s) 837
Eco47I GGWCC 2 cut(s) 410, 745
Eco88I CYCGRG 1 cut(s) 144
EcoO109I RGGNCCY 1 cut(s) 478
EcoRII CCWGG 2 cut(s) 169, 406
EcoT38I GRGCYC 1 cut(s) 437
FaeI CATG 4 cut(s) 134, 544, 690, 917
FatI CATG 4 cut(s) 130, 540, 686, 913
FauI CCCGC 2 cut(s) 662, 856
Fnu4HI GCNGC 2 cut(s) 84, 141
FokI GGATG 4 cut(s) 200, 276, 628, 637
FriOI GRGCYC 1 cut(s) 437
Fsp4HI GCNGC 2 cut(s) 84, 141
FspAI RTGCGCAY 1 cut(s) 566
FspBI CTAG 5 cut(s) 137, 347, 438, 447, 890
FspI TGCGCA 1 cut(s) 566
GlaI GCGC 2 cut(s) 52, 566
GluI GCNGC 2 cut(s) 84, 141
GsaI CCCAGC 1 cut(s) 403
GsuI CTGGAG 2 cut(s) 444, 861
HaeIII GGCC 3 cut(s) 111, 175, 479
HapII CCGG 4 cut(s) 108, 209, 743, 748
HgaI GACGC 1 cut(s) 13
HhaI GCGC 2 cut(s) 53, 567
Hin1II CATG 4 cut(s) 134, 544, 690, 917
Hin6I GCGC 2 cut(s) 51, 565
HinP1I GCGC 2 cut(s) 51, 565
HindIII AAGCTT 1 cut(s) 32
HinfI GANTC 4 cut(s) 23, 378, 814, 886
HpaII CCGG 4 cut(s) 108, 209, 743, 748
HphI GGTGA 3 cut(s) 55, 244, 547
Hpy166II GTNNAC 4 cut(s) 252, 282, 503, 693
Hpy188I TCNGA 3 cut(s) 495, 576, 858
Hpy188III TCNNGA 9 cut(s) 20, 209, 304, 428, 438, 461, 631, 840, 890
Hpy8I GTNNAC 4 cut(s) 252, 282, 503, 693
HpyAV CCTTC 2 cut(s) 265, 778
HpyCH4III ACNGT 6 cut(s) 325, 469, 670, 697, 907, 935
HpyCH4V TGCA 4 cut(s) 184, 686, 913, 938
HpyF10VI GCNNNNNNNGC 1 cut(s) 181
HpyF3I CTNAG 1 cut(s) 776
Hsp92II CATG 4 cut(s) 134, 544, 690, 917
HspAI GCGC 2 cut(s) 51, 565
Kpn2I TCCGGA 1 cut(s) 208
KroI GCCGGC 1 cut(s) 107
KroNI GCCGGC 1 cut(s) 109
Kzo9I GATC 5 cut(s) 69, 486, 543, 627, 853
LmnI GCTCC 1 cut(s) 211
Lsp1109I GCAGC 1 cut(s) 152
LweI GCATC 4 cut(s) 178, 606, 659, 910
MaeI CTAG 5 cut(s) 137, 347, 438, 447, 890
MaeIII GTNAC 4 cut(s) 61, 358, 534, 768
MalI GATC 5 cut(s) 71, 488, 545, 629, 855
MboI GATC 5 cut(s) 69, 486, 543, 627, 853
MboII GAAGA 8 cut(s) 52, 92, 194, 197, 233, 365, 821, 905
MhlI GDGCHC 1 cut(s) 437
MluCI AATT 5 cut(s) 88, 415, 700, 728, 737
MlyI GAGTC 1 cut(s) 32
MroI TCCGGA 1 cut(s) 208
MroNI GCCGGC 1 cut(s) 107
MseI TTAA 2 cut(s) 699, 876
MslI CAYNNNNRTG 4 cut(s) 426, 560, 908, 918
MspA1I CMGCKG 1 cut(s) 851
MspI CCGG 4 cut(s) 108, 209, 743, 748
MspR9I CCNGG 2 cut(s) 171, 408
MvaI CCWGG 2 cut(s) 171, 408
MvnI CGCG 2 cut(s) 21, 86
MwoI GCNNNNNNNGC 1 cut(s) 181
NaeI GCCGGC 1 cut(s) 109
NdeII GATC 5 cut(s) 69, 486, 543, 627, 853
NgoMIV GCCGGC 1 cut(s) 107
NlaIII CATG 4 cut(s) 134, 544, 690, 917
NmeAIII GCCGAG 1 cut(s) 79
NmuCI GTSAC 3 cut(s) 61, 358, 534
NruI TCGCGA 1 cut(s) 21
NsbI TGCGCA 1 cut(s) 566
NspI RCATGY 1 cut(s) 917
NspV TTCGAA 1 cut(s) 376
OliI CACNNNNGTG 2 cut(s) 560, 908
PaeI GCATGC 1 cut(s) 917
PaeR7I CTCGAG 1 cut(s) 144
PdiI GCCGGC 1 cut(s) 109
PfeI GAWTC 3 cut(s) 378, 814, 886
PflMI CCANNNNNTGG 1 cut(s) 36
PkrI GCNGC 2 cut(s) 85, 142
PleI GAGTC 1 cut(s) 31
PpsI GAGTC 1 cut(s) 31
PsiI TTATAA 1 cut(s) 599
Psp6I CCWGG 2 cut(s) 169, 406
PspFI CCCAGC 1 cut(s) 399
PspGI CCWGG 2 cut(s) 169, 406
PspPI GGNCC 4 cut(s) 173, 410, 478, 745
PspXI VCTCGAGB 1 cut(s) 144
PstI CTGCAG 1 cut(s) 186
PstNI CAGNNNCTG 1 cut(s) 467
RruI TCGCGA 1 cut(s) 21
RsaI GTAC 3 cut(s) 125, 504, 692
RsaNI GTAC 3 cut(s) 124, 503, 691
RseI CAYNNNNRTG 4 cut(s) 426, 560, 908, 918
Rsr2I CGGWCCG 1 cut(s) 745
RsrII CGGWCCG 1 cut(s) 745
SaqAI TTAA 2 cut(s) 699, 876
SatI GCNGC 2 cut(s) 84, 141
Sau3AI GATC 5 cut(s) 69, 486, 543, 627, 853
Sau96I GGNCC 4 cut(s) 173, 410, 478, 745
SchI GAGTC 1 cut(s) 32
ScrFI CCNGG 2 cut(s) 171, 408
SduI GDGCHC 1 cut(s) 437
SfaNI GCATC 4 cut(s) 178, 606, 659, 910
SfcI CTRYAG 1 cut(s) 182
Sfr274I CTCGAG 1 cut(s) 144
SfuI TTCGAA 1 cut(s) 376
SinI GGWCC 2 cut(s) 410, 745
SlaI CTCGAG 1 cut(s) 144
SmiMI CAYNNNNRTG 4 cut(s) 426, 560, 908, 918
SmlI CTYRAG 2 cut(s) 144, 870
SmoI CTYRAG 2 cut(s) 144, 870
SphI GCATGC 1 cut(s) 917
Sse9I AATT 5 cut(s) 88, 415, 700, 728, 737
SsiI CCGC 3 cut(s) 84, 655, 849
SspMI CTAG 5 cut(s) 137, 347, 438, 447, 890
StyD4I CCNGG 2 cut(s) 169, 406
TaaI ACNGT 6 cut(s) 325, 469, 670, 697, 907, 935
TaqI TCGA 3 cut(s) 145, 376, 720
TasI AATT 5 cut(s) 88, 415, 700, 728, 737
TatI WGTACW 2 cut(s) 123, 502
TauI GCSGC 1 cut(s) 86
TfiI GAWTC 3 cut(s) 378, 814, 886
Tru1I TTAA 2 cut(s) 699, 876
Tru9I TTAA 2 cut(s) 699, 876
TscAI CASTG 3 cut(s) 186, 244, 700
TseFI GTSAC 3 cut(s) 61, 358, 534
TseI GCWGC 1 cut(s) 140
Tsp45I GTSAC 3 cut(s) 61, 358, 534
TspDTI ATGAA 4 cut(s) 143, 247, 278, 286
TspGWI ACGGA 1 cut(s) 912
TspRI CASTG 3 cut(s) 186, 244, 700
Van91I CCANNNNNTGG 1 cut(s) 36
VpaK11BI GGWCC 2 cut(s) 410, 745
XbaI TCTAGA 2 cut(s) 437, 889
XceI RCATGY 1 cut(s) 917
XhoI CTCGAG 1 cut(s) 144
XspI CTAG 5 cut(s) 137, 347, 438, 447, 890
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.