AT3G58470

S-adenosyl-L-methionine-dependent protein-lysine N- methyltransferase that methylates elongation factor 1-alpha

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0012069
Physical Location & Seq
Reverse (-)
21626671 .. 21628569
1899 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G58470.1

Sequence Viewer

Length: 747 bp
ATGGAAAAAGAAGATGAGCTCCAGAAGTTAAACGGTCAAGTTTCAGCTCCACGAGACGGAGATGTAGAAGATGACGATCCACTAGTGTTGAGTTCTCAAGCTTTGGCGGCGCTTCGGGAATTTCTAGCAGACCAGAACAAGACTGTTGCGAGCACGCCACCGGCGTCTTCCGTCGCCGGAGGAGAAGAATCCGATAAAGTGGAGCTGGTTACGGAGGATTGGAGACTCAGCCAGTTCTGGTACGAACCTGAAACTGCAGAGACTGTAGCAGATGAAGTGGTCACTCTCTCTCAAAGAATTCCAGGCTGCAGAGTCGCTTGCATTGCTTGCCCTACTCTCTACGTTTATCTCAAGAAAAGAGATCCTAGTCTTCAGGTGCAATTGCTGGAGTACGATATGAGATTCGAGAGATATGGAAAAGAGTTCACTTTCTATGATTACAACGAACCAGAAGATCTGCCGCTTCAGCTGAAACATTGCTTCCATATAATCGTTGCAGATCCTCCCTACTTGAGTAGGGAATGCCTGGAAAGAGTTAGTCAAACAATTTTGTTCCTCGCAAGCCCAGTAGATTCATTGTTGCTTCTTCTCACAGGAGAGGTGCAAAGAGAACATGCAGCTGAGCTATTGGGTGTTCGCCCTTGTGTGTTTAAGCCTCATCACTCTAGCAAACTCGGAAACGAGTTCCGACTGTTTATAAGTTATGATCCAGGTACCAGATTAGGAGGCTTGGAAGAAGACAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
N6-adenineMlase

Protein Analysis

248

Amino Acids

28.01

Weight (kDa)

4.58

Isoelectric Point (pI)

46.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
N6-adenineMlase PF10237 72 - 236 1.8e-52 Probable N6-adenine methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012069)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G58470
fragaria_vesca FvH4_6g21270
malus_domestica MD14G1048200.v1.1
prunus_persica Prupe.7G087000_v2.0.a1
pyrus_communis pycom14g03970
rosa_chinensis RchiOBHm_Chr3g0477741 RchiOBHm_Chr3g0477781
rosa_laevigata RLG00000023692
rosa_multiflora Rmu_sc0017665.1_g000001 Rmu_sc0026290.1_g000001
rosa_roxburghii Rroxscaffold_6G00403710
rosa_rugosa Rorug03G0162900 Rorug03G0163000 Rorug03G0163100
rosa_samantha Rh3AG213400 Rh3BG247200 Rh3CG241000 Rh3DG240300
rosa_wichuraiana Rw3G019310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 698
Acc65I GGTACC 1 cut(s) 713
AccB1I GGYRCC 1 cut(s) 713
AciI CCGC 2 cut(s) 107, 461
AclWI GGATC 4 cut(s) 71, 356, 494, 701
AcsI RAATTY 2 cut(s) 119, 297
AcuI CTGAAG 2 cut(s) 356, 449
AcyI GRCGYC 1 cut(s) 164
AfaI GTAC 3 cut(s) 242, 392, 715
AfiI CCNNNNNNNGG 1 cut(s) 56
AhlI ACTAGT 1 cut(s) 82
AjnI CCWGG 3 cut(s) 301, 525, 709
AluBI AGCT 8 cut(s) 19, 47, 101, 205, 469, 620, 625, 744
AluI AGCT 8 cut(s) 19, 47, 101, 205, 469, 620, 625, 744
Alw21I GWGCWC 2 cut(s) 21, 155
Alw26I GTCTC 3 cut(s) 48, 217, 254
AlwI GGATC 4 cut(s) 71, 356, 494, 701
AlwNI CAGNNNCTG 1 cut(s) 263
ApeKI GCWGC 2 cut(s) 306, 617
ApoI RAATTY 2 cut(s) 119, 297
Asp718I GGTACC 1 cut(s) 713
AspLEI GCGC 1 cut(s) 112
BanI GGYRCC 1 cut(s) 713
BanII GRGCYC 1 cut(s) 21
BauI CACGAG 1 cut(s) 51
BbsI GAAGAC 2 cut(s) 159, 362
Bbv12I GWGCWC 2 cut(s) 21, 155
BbvI GCAGC 2 cut(s) 293, 629
BciT130I CCWGG 3 cut(s) 303, 527, 711
BcoDI GTCTC 3 cut(s) 48, 217, 254
BcuI ACTAGT 1 cut(s) 82
BfaI CTAG 5 cut(s) 83, 125, 366, 666, 745
BfmI CTRYAG 3 cut(s) 255, 264, 307
BfoI RGCGCY 1 cut(s) 113
BglII AGATCT 1 cut(s) 454
BisI GCNGC 4 cut(s) 108, 307, 461, 618
BlpI GCTNAGC 1 cut(s) 621
BlsI GCNGC 4 cut(s) 109, 308, 462, 619
Bme1390I CCNGG 3 cut(s) 303, 527, 711
BmiI GGNNCC 1 cut(s) 715
BmrFI CCNGG 3 cut(s) 303, 527, 711
BmrI ACTGGG 1 cut(s) 560
BmuI ACTGGG 1 cut(s) 560
BpiI GAAGAC 2 cut(s) 159, 362
BpmI CTGGAG 2 cut(s) 5, 407
Bpu1102I GCTNAGC 1 cut(s) 621
BpuEI CTTGAG 3 cut(s) 81, 335, 532
BsaBI GATNNNNATC 1 cut(s) 75
BsaHI GRCGYC 1 cut(s) 164
Bsc4I CCNNNNNNNGG 1 cut(s) 56
Bse118I RCCGGY 1 cut(s) 160
Bse1I ACTGG 2 cut(s) 232, 566
Bse3DI GCAATG 2 cut(s) 321, 475
Bse8I GATNNNNATC 1 cut(s) 75
BseBI CCWGG 3 cut(s) 303, 527, 711
BseJI GATNNNNATC 1 cut(s) 75
BseLI CCNNNNNNNGG 1 cut(s) 56
BseMI GCAATG 2 cut(s) 321, 475
BseMII CTCAG 2 cut(s) 241, 612
BseNI ACTGG 2 cut(s) 232, 566
BseRI GAGGAG 1 cut(s) 195
BseXI GCAGC 2 cut(s) 293, 629
BshNI GGYRCC 1 cut(s) 713
BsiHKAI GWGCWC 2 cut(s) 21, 155
BsiSI CCGG 2 cut(s) 161, 177
BslI CCNNNNNNNGG 1 cut(s) 56
BsmAI GTCTC 3 cut(s) 48, 217, 254
BsmBI CGTCTC 1 cut(s) 48
BsmI GAATGC 1 cut(s) 527
Bsp1286I GDGCHC 2 cut(s) 21, 155
Bsp143I GATC 5 cut(s) 76, 361, 454, 499, 706
Bsp1720I GCTNAGC 1 cut(s) 621
BspACI CCGC 2 cut(s) 107, 461
BspCNI CTCAG 2 cut(s) 240, 613
BspLI GGNNCC 1 cut(s) 715
BspMAI CTGCAG 2 cut(s) 259, 311
BspPI GGATC 4 cut(s) 71, 356, 494, 701
BspT107I GGYRCC 1 cut(s) 713
BsrDI GCAATG 2 cut(s) 321, 475
BsrFI RCCGGY 1 cut(s) 160
BsrI ACTGG 2 cut(s) 232, 566
BssAI RCCGGY 1 cut(s) 160
BssMI GATC 5 cut(s) 76, 361, 454, 499, 706
BssNI GRCGYC 1 cut(s) 164
BssSI CACGAG 1 cut(s) 51
Bst2BI CACGAG 1 cut(s) 51
Bst2UI CCWGG 3 cut(s) 303, 527, 711
Bst4CI ACNGT 4 cut(s) 35, 145, 265, 693
BstACI GRCGYC 1 cut(s) 164
BstAPI GCANNNNNTGC 1 cut(s) 327
BstC8I GCNNGC 5 cut(s) 151, 155, 319, 328, 562
BstDEI CTNAG 2 cut(s) 227, 621
BstH2I RGCGCY 1 cut(s) 113
BstHHI GCGC 1 cut(s) 112
BstKTI GATC 5 cut(s) 79, 364, 457, 502, 709
BstMAI GTCTC 3 cut(s) 48, 217, 254
BstMBI GATC 5 cut(s) 76, 361, 454, 499, 706
BstMWI GCNNNNNNNGC 4 cut(s) 107, 323, 327, 466
BstNI CCWGG 3 cut(s) 303, 527, 711
BstNSI RCATGY 1 cut(s) 617
BstSCI CCNGG 3 cut(s) 301, 525, 709
BstSFI CTRYAG 3 cut(s) 255, 264, 307
BstV1I GCAGC 2 cut(s) 293, 629
BstV2I GAAGAC 2 cut(s) 159, 362
BstX2I RGATCY 3 cut(s) 361, 454, 499
BstYI RGATCY 3 cut(s) 361, 454, 499
Cac8I GCNNGC 5 cut(s) 151, 155, 319, 328, 562
CaiI CAGNNNCTG 1 cut(s) 263
CfoI GCGC 1 cut(s) 112
Cfr10I RCCGGY 1 cut(s) 160
CseI GACGC 1 cut(s) 153
Csp6I GTAC 3 cut(s) 241, 391, 714
CspCI CAANNNNNGTGG 2 cut(s) 69, 104
CviAII CATG 1 cut(s) 614
CviQI GTAC 3 cut(s) 241, 391, 714
DdeI CTNAG 2 cut(s) 227, 621
DpnI GATC 5 cut(s) 78, 363, 456, 501, 708
DpnII GATC 5 cut(s) 76, 361, 454, 499, 706
Ecl136II GAGCTC 1 cut(s) 19
Eco24I GRGCYC 1 cut(s) 21
Eco53kI GAGCTC 1 cut(s) 19
Eco57I CTGAAG 2 cut(s) 356, 449
EcoICRI GAGCTC 1 cut(s) 19
EcoRI GAATTC 1 cut(s) 297
EcoRII CCWGG 3 cut(s) 301, 525, 709
EcoT38I GRGCYC 1 cut(s) 21
Esp3I CGTCTC 1 cut(s) 48
FaeI CATG 1 cut(s) 617
FaiI YATR 8 cut(s) 398, 414, 435, 486, 488, 615, 698, 705
FatI CATG 1 cut(s) 613
Fnu4HI GCNGC 4 cut(s) 108, 307, 461, 618
FriOI GRGCYC 1 cut(s) 21
Fsp4HI GCNGC 4 cut(s) 108, 307, 461, 618
FspBI CTAG 5 cut(s) 83, 125, 366, 666, 745
GlaI GCGC 1 cut(s) 111
GluI GCNGC 4 cut(s) 108, 307, 461, 618
GsuI CTGGAG 2 cut(s) 5, 407
HaeII RGCGCY 1 cut(s) 113
HapII CCGG 2 cut(s) 161, 177
HgaI GACGC 1 cut(s) 153
HhaI GCGC 1 cut(s) 112
Hin1I GRCGYC 1 cut(s) 164
Hin1II CATG 1 cut(s) 617
Hin6I GCGC 1 cut(s) 110
HinP1I GCGC 1 cut(s) 110
HindIII AAGCTT 1 cut(s) 99
HinfI GANTC 5 cut(s) 188, 225, 312, 402, 572
HpaII CCGG 2 cut(s) 161, 177
Hpy166II GTNNAC 1 cut(s) 426
Hpy188I TCNGA 3 cut(s) 193, 677, 689
Hpy188III TCNNGA 4 cut(s) 22, 116, 352, 406
Hpy8I GTNNAC 1 cut(s) 426
Hpy99I CGWCG 1 cut(s) 176
HpyCH4III ACNGT 4 cut(s) 35, 145, 265, 693
HpyCH4IV ACGT 1 cut(s) 342
HpyCH4V TGCA 7 cut(s) 257, 309, 321, 379, 497, 604, 617
HpyF10VI GCNNNNNNNGC 4 cut(s) 107, 323, 327, 466
HpyF3I CTNAG 2 cut(s) 227, 621
HpySE526I ACGT 1 cut(s) 342
Hsp92I GRCGYC 1 cut(s) 164
Hsp92II CATG 1 cut(s) 617
HspAI GCGC 1 cut(s) 110
KpnI GGTACC 1 cut(s) 717
Kzo9I GATC 5 cut(s) 76, 361, 454, 499, 706
LmnI GCTCC 3 cut(s) 24, 52, 202
Lsp1109I GCAGC 2 cut(s) 293, 629
MaeI CTAG 5 cut(s) 83, 125, 366, 666, 745
MaeII ACGT 1 cut(s) 342
MaeIII GTNAC 2 cut(s) 208, 280
MalI GATC 5 cut(s) 78, 363, 456, 501, 708
MboI GATC 5 cut(s) 76, 361, 454, 499, 706
MboII GAAGA 8 cut(s) 23, 80, 159, 197, 362, 464, 578, 746
MfeI CAATTG 1 cut(s) 380
MflI RGATCY 3 cut(s) 361, 454, 499
MhlI GDGCHC 2 cut(s) 21, 155
MluCI AATT 4 cut(s) 119, 297, 380, 546
MlyI GAGTC 2 cut(s) 219, 321
MmeI TCCRAC 1 cut(s) 712
MnlI CCTC 7 cut(s) 173, 208, 513, 566, 592, 666, 719
MseI TTAA 2 cut(s) 29, 651
MspA1I CMGCKG 2 cut(s) 469, 620
MspI CCGG 2 cut(s) 161, 177
MspR9I CCNGG 3 cut(s) 303, 527, 711
MunI CAATTG 1 cut(s) 380
Mva1269I GAATGC 1 cut(s) 527
MvaI CCWGG 3 cut(s) 303, 527, 711
MwoI GCNNNNNNNGC 4 cut(s) 107, 323, 327, 466
NdeII GATC 5 cut(s) 76, 361, 454, 499, 706
NlaIII CATG 1 cut(s) 617
NlaIV GGNNCC 1 cut(s) 715
NmuCI GTSAC 1 cut(s) 280
NspI RCATGY 1 cut(s) 617
PcsI WCGNNNNNNNCGW 1 cut(s) 161
PctI GAATGC 1 cut(s) 527
PfeI GAWTC 3 cut(s) 188, 402, 572
PkrI GCNGC 4 cut(s) 109, 308, 462, 619
PleI GAGTC 2 cut(s) 219, 320
PpsI GAGTC 2 cut(s) 219, 320
PsiI TTATAA 1 cut(s) 698
Psp124BI GAGCTC 1 cut(s) 21
Psp6I CCWGG 3 cut(s) 301, 525, 709
PspGI CCWGG 3 cut(s) 301, 525, 709
PspN4I GGNNCC 1 cut(s) 715
PstI CTGCAG 2 cut(s) 259, 311
PstNI CAGNNNCTG 1 cut(s) 263
PsuI RGATCY 3 cut(s) 361, 454, 499
PvuII CAGCTG 2 cut(s) 469, 620
RsaI GTAC 3 cut(s) 242, 392, 715
RsaNI GTAC 3 cut(s) 241, 391, 714
SacI GAGCTC 1 cut(s) 21
SaqAI TTAA 2 cut(s) 29, 651
SatI GCNGC 4 cut(s) 108, 307, 461, 618
Sau3AI GATC 5 cut(s) 76, 361, 454, 499, 706
SchI GAGTC 2 cut(s) 219, 321
ScrFI CCNGG 3 cut(s) 303, 527, 711
SduI GDGCHC 2 cut(s) 21, 155
SfcI CTRYAG 3 cut(s) 255, 264, 307
SgrAI CRCCGGYG 1 cut(s) 160
SmlI CTYRAG 3 cut(s) 96, 350, 511
SmoI CTYRAG 3 cut(s) 96, 350, 511
SpeI ACTAGT 1 cut(s) 82
Sse9I AATT 4 cut(s) 119, 297, 380, 546
SsiI CCGC 2 cut(s) 107, 461
SspMI CTAG 5 cut(s) 83, 125, 366, 666, 745
SstI GAGCTC 1 cut(s) 21
StyD4I CCNGG 3 cut(s) 301, 525, 709
TaaI ACNGT 4 cut(s) 35, 145, 265, 693
TaiI ACGT 1 cut(s) 345
TaqI TCGA 1 cut(s) 405
TasI AATT 4 cut(s) 119, 297, 380, 546
TauI GCSGC 2 cut(s) 110, 463
TfiI GAWTC 3 cut(s) 188, 402, 572
Tru1I TTAA 2 cut(s) 29, 651
Tru9I TTAA 2 cut(s) 29, 651
TseFI GTSAC 1 cut(s) 280
TseI GCWGC 2 cut(s) 306, 617
Tsp45I GTSAC 1 cut(s) 280
TspDTI ATGAA 2 cut(s) 288, 564
TspGWI ACGGA 3 cut(s) 72, 160, 227
XapI RAATTY 2 cut(s) 119, 297
XceI RCATGY 1 cut(s) 617
XspI CTAG 5 cut(s) 83, 125, 366, 666, 745
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.