FvH4_6g21270

S-adenosyl-L-methionine-dependent protein-lysine N- methyltransferase that methylates elongation factor 1-alpha

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0012069
Physical Location & Seq
Reverse (-)
14822463 .. 14824650
2188 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g21270.t1

Sequence Viewer

Length: 699 bp
ATGGAACAAGAACTCCCTCAAACCGTCTCCGGGCACCAAAACGACGCCGTAGAAGACGATGACGACGCACCGACTCTAAGCCTTGAAGCCTTGGAAGCTCTCAAGGAGTTCCTGACCGAGCAAAGCCACTCCCTAAACGACACCGCCGGCGAAACAGCTCCGGCTCTGATCTCGGAGGACTGGCGGATGAGCCAGTTCTGGTATGACCACCACACCGCCCAAACCCTAGCCCAAGAGGTTCTCACTCTCCGCAAATCGGACTCCACTCGAGTCGCCTGTGTCGCCTGTCCCTCTCTCTACGCCTATCTCAAGAATATTGATTCGGATGTGTCTGTACAACTGCTTGAGTATGATACAAGGTTTGAGCAGTACGGGAGTGATTTCACGTTCTATGATTATAATCAACCGGAAGATTTGCCTTTCGAGCTCAAGCATGGTTTTGATATTGTAGTTGCTGATCCTCCTTATCTGAGTCAGGAATGCTTGGAGAAAGTCTCTAAAACTGTTTCGTTTCTTGCGCGACCGGAAAATCCTTACCTGCTCCTCCTCACAGGAGAGGTGCAGAAAGAGAGAGCAGCTGAGCTCATGGGATTGCATCCATGTGGTTTTAGGCCTCGGCACTCCAGCAAACTAGGAAATGAGTTTCGGTTGTTCACAAGTTATGATCCTGAGACTAGATTAGGAGGGTGGGACAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
N6-adenineMlase

Protein Analysis

233

Amino Acids

26.41

Weight (kDa)

4.46

Isoelectric Point (pI)

49.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
N6-adenineMlase PF10237 59 - 222 1.9e-57 Probable N6-adenine methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012069)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G58470
fragaria_vesca FvH4_6g21270
malus_domestica MD14G1048200.v1.1
prunus_persica Prupe.7G087000_v2.0.a1
pyrus_communis pycom14g03970
rosa_chinensis RchiOBHm_Chr3g0477741 RchiOBHm_Chr3g0477781
rosa_laevigata RLG00000023692
rosa_multiflora Rmu_sc0017665.1_g000001 Rmu_sc0026290.1_g000001
rosa_roxburghii Rroxscaffold_6G00403710
rosa_rugosa Rorug03G0162900 Rorug03G0163000 Rorug03G0163100
rosa_samantha Rh3AG213400 Rh3BG247200 Rh3CG241000 Rh3DG240300
rosa_wichuraiana Rw3G019310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 399
Acc36I ACCTGC 1 cut(s) 546
AccB1I GGYRCC 1 cut(s) 33
AccII CGCG 1 cut(s) 520
AciI CCGC 4 cut(s) 144, 184, 216, 250
AclWI GGATC 2 cut(s) 452, 659
AcyI GRCGYC 1 cut(s) 45
AfaI GTAC 2 cut(s) 336, 371
AfiI CCNNNNNNNGG 2 cut(s) 30, 256
AgsI TTSAA 1 cut(s) 86
AloI GAACNNNNNNTCC 1 cut(s) 29
AluBI AGCT 5 cut(s) 98, 158, 427, 578, 583
AluI AGCT 5 cut(s) 98, 158, 427, 578, 583
Alw21I GWGCWC 2 cut(s) 429, 585
Alw26I GTCTC 3 cut(s) 31, 499, 665
AlwI GGATC 2 cut(s) 452, 659
Ama87I CYCGRG 1 cut(s) 267
AoxI GGCC 1 cut(s) 611
ApeKI GCWGC 1 cut(s) 575
AspLEI GCGC 1 cut(s) 520
AsuC2I CCSGG 1 cut(s) 31
AvaI CYCGRG 1 cut(s) 267
BaeGI GKGCMC 1 cut(s) 36
BanI GGYRCC 1 cut(s) 33
BanII GRGCYC 2 cut(s) 429, 585
BbsI GAAGAC 1 cut(s) 60
Bbv12I GWGCWC 2 cut(s) 429, 585
BbvI GCAGC 1 cut(s) 587
BceAI ACGGC 1 cut(s) 32
BcnI CCSGG 1 cut(s) 31
BcoDI GTCTC 3 cut(s) 31, 499, 665
BfaI CTAG 3 cut(s) 227, 632, 675
BfuAI ACCTGC 1 cut(s) 546
BisI GCNGC 1 cut(s) 576
BlpI GCTNAGC 1 cut(s) 579
BlsI GCNGC 1 cut(s) 577
Bme1390I CCNGG 1 cut(s) 31
BmeT110I CYCGRG 1 cut(s) 267
BmiI GGNNCC 1 cut(s) 35
BmrFI CCNGG 1 cut(s) 31
BmsI GCATC 1 cut(s) 604
BpiI GAAGAC 1 cut(s) 60
BplI GAGNNNNNCTC 2 cut(s) 479, 511
BpmI CTGGAG 1 cut(s) 607
Bpu1102I GCTNAGC 1 cut(s) 579
BpuEI CTTGAG 4 cut(s) 86, 293, 365, 413
BpuMI CCSGG 1 cut(s) 31
BsaBI GATNNNNATC 1 cut(s) 399
BsaHI GRCGYC 1 cut(s) 45
BsaJI CCNNGG 2 cut(s) 90, 614
BsaWI WCCGGW 2 cut(s) 406, 523
BsaXI ACNNNNNCTCC 1 cut(s) 27
Bsc4I CCNNNNNNNGG 2 cut(s) 30, 256
Bse118I RCCGGY 1 cut(s) 146
Bse1I ACTGG 2 cut(s) 185, 193
Bse8I GATNNNNATC 1 cut(s) 399
BseDI CCNNGG 2 cut(s) 90, 614
BseGI GGATG 3 cut(s) 192, 331, 595
BseJI GATNNNNATC 1 cut(s) 399
BseLI CCNNNNNNNGG 2 cut(s) 30, 256
BseMII CTCAG 3 cut(s) 461, 570, 660
BseNI ACTGG 2 cut(s) 185, 193
BseRI GAGGAG 2 cut(s) 533, 536
BseSI GKGCMC 1 cut(s) 36
BseXI GCAGC 1 cut(s) 587
BsgI GTGCAG 1 cut(s) 581
Bsh1236I CGCG 1 cut(s) 520
Bsh1285I CGRYCG 1 cut(s) 524
BshFI GGCC 1 cut(s) 613
BshNI GGYRCC 1 cut(s) 33
BsiEI CGRYCG 1 cut(s) 524
BsiHKAI GWGCWC 2 cut(s) 429, 585
BsiHKCI CYCGRG 1 cut(s) 267
BsiSI CCGG 5 cut(s) 30, 147, 161, 407, 524
BslFI GGGAC 1 cut(s) 273
BslI CCNNNNNNNGG 2 cut(s) 30, 256
BsmAI GTCTC 3 cut(s) 31, 499, 665
BsmBI CGTCTC 1 cut(s) 31
BsmFI GGGAC 1 cut(s) 273
BsmI GAATGC 1 cut(s) 485
BsnI GGCC 1 cut(s) 613
BsoBI CYCGRG 1 cut(s) 267
Bsp1286I GDGCHC 3 cut(s) 36, 429, 585
Bsp1407I TGTACA 1 cut(s) 334
Bsp143I GATC 3 cut(s) 168, 457, 664
Bsp1720I GCTNAGC 1 cut(s) 579
BspACI CCGC 4 cut(s) 144, 184, 216, 250
BspANI GGCC 1 cut(s) 613
BspCNI CTCAG 3 cut(s) 462, 571, 661
BspFNI CGCG 1 cut(s) 520
BspLI GGNNCC 1 cut(s) 35
BspMI ACCTGC 1 cut(s) 546
BspPI GGATC 2 cut(s) 452, 659
BspT107I GGYRCC 1 cut(s) 33
BsrFI RCCGGY 1 cut(s) 146
BsrGI TGTACA 1 cut(s) 334
BsrI ACTGG 2 cut(s) 185, 193
BssAI RCCGGY 1 cut(s) 146
BssECI CCNNGG 2 cut(s) 90, 614
BssMI GATC 3 cut(s) 168, 457, 664
BssNI GRCGYC 1 cut(s) 45
BssT1I CCWWGG 1 cut(s) 90
Bst4CI ACNGT 2 cut(s) 25, 505
BstACI GRCGYC 1 cut(s) 45
BstAUI TGTACA 1 cut(s) 334
BstC8I GCNNGC 1 cut(s) 148
BstDEI CTNAG 4 cut(s) 77, 470, 579, 669
BstF5I GGATG 3 cut(s) 192, 331, 595
BstFNI CGCG 1 cut(s) 520
BstHHI GCGC 1 cut(s) 520
BstKTI GATC 3 cut(s) 171, 460, 667
BstMAI GTCTC 3 cut(s) 31, 499, 665
BstMBI GATC 3 cut(s) 168, 457, 664
BstMCI CGRYCG 1 cut(s) 524
BstMWI GCNNNNNNNGC 3 cut(s) 95, 281, 424
BstSCI CCNGG 1 cut(s) 29
BstSLI GKGCMC 1 cut(s) 36
BstUI CGCG 1 cut(s) 520
BstV1I GCAGC 1 cut(s) 587
BstV2I GAAGAC 1 cut(s) 60
BsuRI GGCC 1 cut(s) 613
BtsCI GGATG 3 cut(s) 192, 331, 595
BveI ACCTGC 1 cut(s) 546
Cac8I GCNNGC 1 cut(s) 148
CfoI GCGC 1 cut(s) 520
Cfr10I RCCGGY 1 cut(s) 146
CseI GACGC 2 cut(s) 53, 74
Csp6I GTAC 2 cut(s) 335, 370
CviAII CATG 3 cut(s) 434, 586, 600
CviQI GTAC 2 cut(s) 335, 370
DdeI CTNAG 4 cut(s) 77, 470, 579, 669
DpnI GATC 3 cut(s) 170, 459, 666
DpnII GATC 3 cut(s) 168, 457, 664
EciI GGCGGA 1 cut(s) 199
Ecl136II GAGCTC 2 cut(s) 427, 583
Eco130I CCWWGG 1 cut(s) 90
Eco147I AGGCCT 1 cut(s) 613
Eco24I GRGCYC 2 cut(s) 429, 585
Eco53kI GAGCTC 2 cut(s) 427, 583
Eco88I CYCGRG 1 cut(s) 267
EcoICRI GAGCTC 2 cut(s) 427, 583
EcoT14I CCWWGG 1 cut(s) 90
EcoT38I GRGCYC 2 cut(s) 429, 585
ErhI CCWWGG 1 cut(s) 90
Esp3I CGTCTC 1 cut(s) 31
FaeI CATG 3 cut(s) 437, 589, 603
FaiI YATR 8 cut(s) 204, 351, 393, 399, 435, 587, 601, 663
FaqI GGGAC 1 cut(s) 273
FatI CATG 3 cut(s) 433, 585, 599
Fnu4HI GCNGC 1 cut(s) 576
FokI GGATG 3 cut(s) 199, 338, 582
FriOI GRGCYC 2 cut(s) 429, 585
Fsp4HI GCNGC 1 cut(s) 576
FspBI CTAG 3 cut(s) 227, 632, 675
GlaI GCGC 1 cut(s) 519
GluI GCNGC 1 cut(s) 576
GsuI CTGGAG 1 cut(s) 607
HaeIII GGCC 1 cut(s) 613
HapII CCGG 5 cut(s) 30, 147, 161, 407, 524
HgaI GACGC 2 cut(s) 53, 74
HhaI GCGC 1 cut(s) 520
Hin1I GRCGYC 1 cut(s) 45
Hin1II CATG 3 cut(s) 437, 589, 603
Hin6I GCGC 1 cut(s) 518
HinP1I GCGC 1 cut(s) 518
HinfI GANTC 5 cut(s) 73, 260, 270, 320, 472
HpaII CCGG 5 cut(s) 30, 147, 161, 407, 524
Hpy166II GTNNAC 1 cut(s) 654
Hpy188I TCNGA 5 cut(s) 168, 175, 259, 325, 471
Hpy188III TCNNGA 4 cut(s) 112, 310, 476, 668
Hpy8I GTNNAC 1 cut(s) 654
Hpy99I CGWCG 2 cut(s) 47, 68
HpyCH4III ACNGT 2 cut(s) 25, 505
HpyCH4IV ACGT 1 cut(s) 386
HpyCH4V TGCA 2 cut(s) 562, 595
HpyF10VI GCNNNNNNNGC 3 cut(s) 95, 281, 424
HpyF3I CTNAG 4 cut(s) 77, 470, 579, 669
HpySE526I ACGT 1 cut(s) 386
Hsp92I GRCGYC 1 cut(s) 45
Hsp92II CATG 3 cut(s) 437, 589, 603
HspAI GCGC 1 cut(s) 518
KroI GCCGGC 1 cut(s) 146
KroNI GCCGGC 1 cut(s) 148
Kzo9I GATC 3 cut(s) 168, 457, 664
LmnI GCTCC 2 cut(s) 163, 546
Lsp1109I GCAGC 1 cut(s) 587
LweI GCATC 1 cut(s) 604
MaeI CTAG 3 cut(s) 227, 632, 675
MaeII ACGT 1 cut(s) 386
MalI GATC 3 cut(s) 170, 459, 666
MboI GATC 3 cut(s) 168, 457, 664
MboII GAAGA 2 cut(s) 65, 422
MhlI GDGCHC 3 cut(s) 36, 429, 585
MluCI AATT 1 cut(s) 694
MlyI GAGTC 4 cut(s) 67, 254, 279, 481
MreI CGCCGGCG 1 cut(s) 146
MroNI GCCGGC 1 cut(s) 146
MslI CAYNNNNRTG 1 cut(s) 600
MspA1I CMGCKG 1 cut(s) 578
MspI CCGG 5 cut(s) 30, 147, 161, 407, 524
MspR9I CCNGG 1 cut(s) 31
Mva1269I GAATGC 1 cut(s) 485
MvnI CGCG 1 cut(s) 520
MwoI GCNNNNNNNGC 3 cut(s) 95, 281, 424
NaeI GCCGGC 1 cut(s) 148
NciI CCSGG 1 cut(s) 31
NdeII GATC 3 cut(s) 168, 457, 664
NgoMIV GCCGGC 1 cut(s) 146
NlaIII CATG 3 cut(s) 437, 589, 603
NlaIV GGNNCC 1 cut(s) 35
NmeAIII GCCGAG 1 cut(s) 595
PaeR7I CTCGAG 1 cut(s) 267
PceI AGGCCT 1 cut(s) 613
PctI GAATGC 1 cut(s) 485
PdiI GCCGGC 1 cut(s) 148
PfeI GAWTC 1 cut(s) 320
PkrI GCNGC 1 cut(s) 577
PleI GAGTC 4 cut(s) 67, 254, 278, 480
PpsI GAGTC 4 cut(s) 67, 254, 278, 480
PsiI TTATAA 1 cut(s) 399
Psp124BI GAGCTC 2 cut(s) 429, 585
PspN4I GGNNCC 1 cut(s) 35
PspXI VCTCGAGB 1 cut(s) 267
PvuII CAGCTG 1 cut(s) 578
RsaI GTAC 2 cut(s) 336, 371
RsaNI GTAC 2 cut(s) 335, 370
RseI CAYNNNNRTG 1 cut(s) 600
SacI GAGCTC 2 cut(s) 429, 585
SatI GCNGC 1 cut(s) 576
Sau3AI GATC 3 cut(s) 168, 457, 664
SchI GAGTC 4 cut(s) 67, 254, 279, 481
ScrFI CCNGG 1 cut(s) 31
SduI GDGCHC 3 cut(s) 36, 429, 585
SfaNI GCATC 1 cut(s) 604
Sfr274I CTCGAG 1 cut(s) 267
SgrAI CRCCGGYG 1 cut(s) 146
SlaI CTCGAG 1 cut(s) 267
SmiMI CAYNNNNRTG 1 cut(s) 600
SmlI CTYRAG 5 cut(s) 101, 267, 308, 344, 428
SmoI CTYRAG 5 cut(s) 101, 267, 308, 344, 428
Sse9I AATT 1 cut(s) 694
SseBI AGGCCT 1 cut(s) 613
SsiI CCGC 4 cut(s) 144, 184, 216, 250
SspI AATATT 1 cut(s) 316
SspMI CTAG 3 cut(s) 227, 632, 675
SstI GAGCTC 2 cut(s) 429, 585
StuI AGGCCT 1 cut(s) 613
StyD4I CCNGG 1 cut(s) 29
StyI CCWWGG 1 cut(s) 90
TaaI ACNGT 2 cut(s) 25, 505
TaiI ACGT 1 cut(s) 389
TaqI TCGA 2 cut(s) 268, 423
TaqII GACCGA 1 cut(s) 131
TasI AATT 1 cut(s) 694
TatI WGTACW 1 cut(s) 334
TfiI GAWTC 1 cut(s) 320
TseI GCWGC 1 cut(s) 575
XhoI CTCGAG 1 cut(s) 267
XspI CTAG 3 cut(s) 227, 632, 675
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.