Rh3DG240300

S-adenosyl-L-methionine-dependent protein-lysine N- methyltransferase that methylates elongation factor 1-alpha

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0012069
Physical Location & Seq
Reverse (-)
23113385 .. 23117461
4077 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG240300.1

Sequence Viewer

Length: 639 bp
ATGTTTGGTCACGCAATTTGTAAATGGATCTCTTGCGAAAATTCATTAGACAGGTACAAACTAAATTCAGAGGCCGTTCCCTTAAACCCTTTCTTCACTACTAGACACTTTGGTCTACATCGCCACCAACAAATGGAACAAGACCTCCTCCCCAATTCAATCTCCGGCCACCAAAACGACGATGATGACGCACCGACTTTAAGCCTTCAAGCGTTAGAAGCTCTCAAGGAGTTCCTCACCGAGCAAAGCCAGTCCTCAAACGACACCGCCGCCGAAGCAGACTCGGCGCCGGCTTTGATCTCGGAGGACTGGCGGATGAGCCAGTTCTGGTACGACCACCACACGGCCCAAACCCTAGCCGAAGAGGTCCTCAGTCTCTGCAAATCGGACTCCACTCGAGTCGCCTGCGTCGCTTGTCCCTCGCTCTACGCCTATCTCAAGAGTATTGATTCTCATGTGTCTGTACAACTGCTGGAGTATGATAGAAGGTTTGAGCAATACGGGAGTGATTTCACGTTTTATGATTACAACCAACCAGAAGACTTGCCTTTGGAGCTAAAGCATGGCTTTGATGTTGTAGTTGCTGATCCTCCTTATCTGGTGAGCTACATTATGCGCATTCGTCCTTATTGGCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
N6-adenineMlase

Protein Analysis

212

Amino Acids

24.42

Weight (kDa)

4.7

Isoelectric Point (pI)

54.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
N6-adenineMlase PF10237 102 - 204 1.9e-37 Probable N6-adenine methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012069)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G58470
fragaria_vesca FvH4_6g21270
malus_domestica MD14G1048200.v1.1
prunus_persica Prupe.7G087000_v2.0.a1
pyrus_communis pycom14g03970
rosa_chinensis RchiOBHm_Chr3g0477741 RchiOBHm_Chr3g0477781
rosa_laevigata RLG00000023692
rosa_multiflora Rmu_sc0017665.1_g000001 Rmu_sc0026290.1_g000001
rosa_roxburghii Rroxscaffold_6G00403710
rosa_rugosa Rorug03G0162900 Rorug03G0163000 Rorug03G0163100
rosa_samantha Rh3AG213400 Rh3BG247200 Rh3CG241000 Rh3DG240300
rosa_wichuraiana Rw3G019310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 111
Acc16I TGCGCA 1 cut(s) 617
AccB1I GGYRCC 1 cut(s) 286
AccB7I CCANNNNNTGG 1 cut(s) 133
AccI GTMKAC 1 cut(s) 115
AciI CCGC 3 cut(s) 267, 270, 313
AclWI GGATC 2 cut(s) 35, 581
AcoI YGGCCR 1 cut(s) 166
AcsI RAATTY 2 cut(s) 40, 64
AcyI GRCGYC 1 cut(s) 287
AfaI GTAC 3 cut(s) 56, 332, 465
AfiI CCNNNNNNNGG 2 cut(s) 133, 343
AgsI TTSAA 2 cut(s) 159, 209
AloI GAACNNNNNNTCC 2 cut(s) 129, 161
AluBI AGCT 3 cut(s) 221, 556, 606
AluI AGCT 3 cut(s) 221, 556, 606
Alw26I GTCTC 1 cut(s) 380
AlwI GGATC 2 cut(s) 35, 581
AlwNI CAGNNNCTG 1 cut(s) 378
Ama87I CYCGRG 1 cut(s) 396
AoxI GGCC 3 cut(s) 72, 166, 345
ApoI RAATTY 2 cut(s) 40, 64
AspLEI GCGC 2 cut(s) 289, 618
AspS9I GGNCC 2 cut(s) 346, 367
AsuHPI GGTGA 2 cut(s) 229, 613
AvaI CYCGRG 1 cut(s) 396
AvaII GGWCC 1 cut(s) 367
BanI GGYRCC 1 cut(s) 286
BbsI GAAGAC 1 cut(s) 546
BceAI ACGGC 2 cut(s) 59, 360
BcgI CGANNNNNNTGC 2 cut(s) 387, 421
BcoDI GTCTC 1 cut(s) 380
BfaI CTAG 2 cut(s) 102, 356
BfoI RGCGCY 1 cut(s) 290
BisI GCNGC 1 cut(s) 270
BlsI GCNGC 1 cut(s) 271
Bme18I GGWCC 1 cut(s) 367
BmeT110I CYCGRG 1 cut(s) 396
BmgT120I GGNCC 2 cut(s) 346, 367
BmiI GGNNCC 1 cut(s) 288
BpiI GAAGAC 1 cut(s) 546
BpmI CTGGAG 1 cut(s) 494
BpuEI CTTGAG 2 cut(s) 209, 422
BsaHI GRCGYC 1 cut(s) 287
BsaXI ACNNNNNCTCC 2 cut(s) 129, 159
Bsc4I CCNNNNNNNGG 2 cut(s) 133, 343
Bse118I RCCGGY 1 cut(s) 289
Bse1I ACTGG 3 cut(s) 250, 314, 322
BseGI GGATG 1 cut(s) 321
BseLI CCNNNNNNNGG 2 cut(s) 133, 343
BseMII CTCAG 1 cut(s) 385
BseNI ACTGG 3 cut(s) 250, 314, 322
BseRI GAGGAG 1 cut(s) 137
BshFI GGCC 3 cut(s) 74, 168, 347
BshNI GGYRCC 1 cut(s) 286
BsiHKCI CYCGRG 1 cut(s) 396
BsiSI CCGG 2 cut(s) 165, 290
BslFI GGGAC 1 cut(s) 402
BslI CCNNNNNNNGG 2 cut(s) 133, 343
BsmAI GTCTC 1 cut(s) 380
BsmFI GGGAC 1 cut(s) 402
BsmI GAATGC 1 cut(s) 618
BsnI GGCC 3 cut(s) 74, 168, 347
BsoBI CYCGRG 1 cut(s) 396
Bsp1407I TGTACA 1 cut(s) 463
Bsp143I GATC 3 cut(s) 27, 297, 586
BspACI CCGC 3 cut(s) 267, 270, 313
BspANI GGCC 3 cut(s) 74, 168, 347
BspCNI CTCAG 1 cut(s) 384
BspLI GGNNCC 1 cut(s) 288
BspPI GGATC 2 cut(s) 35, 581
BspT107I GGYRCC 1 cut(s) 286
BsrFI RCCGGY 1 cut(s) 289
BsrGI TGTACA 1 cut(s) 463
BsrI ACTGG 3 cut(s) 250, 314, 322
BssAI RCCGGY 1 cut(s) 289
BssMI GATC 3 cut(s) 27, 297, 586
BssNI GRCGYC 1 cut(s) 287
Bst6I CTCTTC 1 cut(s) 357
BstACI GRCGYC 1 cut(s) 287
BstAUI TGTACA 1 cut(s) 463
BstC8I GCNNGC 2 cut(s) 291, 406
BstDEI CTNAG 1 cut(s) 371
BstF5I GGATG 1 cut(s) 321
BstH2I RGCGCY 1 cut(s) 290
BstHHI GCGC 2 cut(s) 289, 618
BstKTI GATC 3 cut(s) 30, 300, 589
BstMAI GTCTC 1 cut(s) 380
BstMBI GATC 3 cut(s) 27, 297, 586
BstMWI GCNNNNNNNGC 5 cut(s) 218, 275, 284, 410, 553
BstV2I GAAGAC 1 cut(s) 546
BstX2I RGATCY 1 cut(s) 27
BstYI RGATCY 1 cut(s) 27
BsuRI GGCC 3 cut(s) 74, 168, 347
BtgZI GCGATG 1 cut(s) 104
BtsCI GGATG 1 cut(s) 321
Cac8I GCNNGC 2 cut(s) 291, 406
CaiI CAGNNNCTG 1 cut(s) 378
CfoI GCGC 2 cut(s) 289, 618
Cfr10I RCCGGY 1 cut(s) 289
Cfr13I GGNCC 2 cut(s) 346, 367
CseI GACGC 2 cut(s) 197, 397
Csp6I GTAC 3 cut(s) 55, 331, 464
CviAII CATG 2 cut(s) 455, 563
CviQI GTAC 3 cut(s) 55, 331, 464
DdeI CTNAG 1 cut(s) 371
DinI GGCGCC 1 cut(s) 288
DpnI GATC 3 cut(s) 29, 299, 588
DpnII GATC 3 cut(s) 27, 297, 586
DrdI GACNNNNNNGTC 1 cut(s) 111
DseDI GACNNNNNNGTC 1 cut(s) 111
EaeI YGGCCR 1 cut(s) 166
Eam1104I CTCTTC 1 cut(s) 357
EarI CTCTTC 1 cut(s) 357
EciI GGCGGA 1 cut(s) 328
Eco47I GGWCC 1 cut(s) 367
Eco88I CYCGRG 1 cut(s) 396
EcoO109I RGGNCCY 1 cut(s) 367
EgeI GGCGCC 1 cut(s) 288
EheI GGCGCC 1 cut(s) 288
FaeI CATG 2 cut(s) 458, 566
FaiI YATR 5 cut(s) 456, 480, 522, 564, 614
FalI AAGNNNNNCTT 2 cut(s) 551, 583
FaqI GGGAC 1 cut(s) 402
FatI CATG 2 cut(s) 454, 562
FblI GTMKAC 1 cut(s) 115
Fnu4HI GCNGC 1 cut(s) 270
FokI GGATG 1 cut(s) 328
Fsp4HI GCNGC 1 cut(s) 270
FspAI RTGCGCAY 1 cut(s) 617
FspBI CTAG 2 cut(s) 102, 356
FspI TGCGCA 1 cut(s) 617
GlaI GCGC 2 cut(s) 288, 617
GluI GCNGC 1 cut(s) 270
GsuI CTGGAG 1 cut(s) 494
HaeII RGCGCY 1 cut(s) 290
HaeIII GGCC 3 cut(s) 74, 168, 347
HapII CCGG 2 cut(s) 165, 290
HgaI GACGC 2 cut(s) 197, 397
HhaI GCGC 2 cut(s) 289, 618
Hin1I GRCGYC 1 cut(s) 287
Hin1II CATG 2 cut(s) 458, 566
Hin6I GCGC 2 cut(s) 287, 616
HinP1I GCGC 2 cut(s) 287, 616
HinfI GANTC 4 cut(s) 281, 389, 399, 449
HpaII CCGG 2 cut(s) 165, 290
HphI GGTGA 2 cut(s) 229, 613
Hpy166II GTNNAC 1 cut(s) 116
Hpy188I TCNGA 3 cut(s) 70, 304, 388
Hpy188III TCNNGA 1 cut(s) 439
Hpy8I GTNNAC 1 cut(s) 116
Hpy99I CGWCG 2 cut(s) 182, 413
HpyAV CCTTC 2 cut(s) 215, 480
HpyCH4IV ACGT 1 cut(s) 515
HpyCH4V TGCA 1 cut(s) 381
HpyF10VI GCNNNNNNNGC 5 cut(s) 218, 275, 284, 410, 553
HpyF3I CTNAG 1 cut(s) 371
HpySE526I ACGT 1 cut(s) 515
Hsp92I GRCGYC 1 cut(s) 287
Hsp92II CATG 2 cut(s) 458, 566
HspAI GCGC 2 cut(s) 287, 616
KasI GGCGCC 1 cut(s) 286
KroI GCCGGC 1 cut(s) 289
KroNI GCCGGC 1 cut(s) 291
Kzo9I GATC 3 cut(s) 27, 297, 586
LmnI GCTCC 1 cut(s) 553
MaeI CTAG 2 cut(s) 102, 356
MaeII ACGT 1 cut(s) 515
MaeIII GTNAC 1 cut(s) 8
MalI GATC 3 cut(s) 29, 299, 588
MboI GATC 3 cut(s) 27, 297, 586
MboII GAAGA 3 cut(s) 85, 374, 551
MflI RGATCY 1 cut(s) 27
MluCI AATT 4 cut(s) 15, 40, 64, 154
Mly113I GGCGCC 1 cut(s) 287
MlyI GAGTC 3 cut(s) 275, 383, 408
MroNI GCCGGC 1 cut(s) 289
MseI TTAA 2 cut(s) 83, 200
MspI CCGG 2 cut(s) 165, 290
Mva1269I GAATGC 1 cut(s) 618
MwoI GCNNNNNNNGC 5 cut(s) 218, 275, 284, 410, 553
NaeI GCCGGC 1 cut(s) 291
NarI GGCGCC 1 cut(s) 287
NdeII GATC 3 cut(s) 27, 297, 586
NgoMIV GCCGGC 1 cut(s) 289
NlaIII CATG 2 cut(s) 458, 566
NlaIV GGNNCC 1 cut(s) 288
NmeAIII GCCGAG 1 cut(s) 263
NmuCI GTSAC 1 cut(s) 8
NsbI TGCGCA 1 cut(s) 617
PaeR7I CTCGAG 1 cut(s) 396
PctI GAATGC 1 cut(s) 618
PdiI GCCGGC 1 cut(s) 291
PfeI GAWTC 1 cut(s) 449
PflMI CCANNNNNTGG 1 cut(s) 133
PkrI GCNGC 1 cut(s) 271
PleI GAGTC 3 cut(s) 275, 383, 407
PluTI GGCGCC 1 cut(s) 290
PpsI GAGTC 3 cut(s) 275, 383, 407
PpuMI RGGWCCY 1 cut(s) 367
Psp5II RGGWCCY 1 cut(s) 367
PspN4I GGNNCC 1 cut(s) 288
PspPI GGNCC 2 cut(s) 346, 367
PspPPI RGGWCCY 1 cut(s) 367
PspXI VCTCGAGB 1 cut(s) 396
PstNI CAGNNNCTG 1 cut(s) 378
PsuI RGATCY 1 cut(s) 27
RsaI GTAC 3 cut(s) 56, 332, 465
RsaNI GTAC 3 cut(s) 55, 331, 464
SaqAI TTAA 2 cut(s) 83, 200
SatI GCNGC 1 cut(s) 270
Sau3AI GATC 3 cut(s) 27, 297, 586
Sau96I GGNCC 2 cut(s) 346, 367
SchI GAGTC 3 cut(s) 275, 383, 408
SetI ASST 8 cut(s) 56, 147, 223, 369, 491, 518, 558, 608
SfoI GGCGCC 1 cut(s) 288
Sfr274I CTCGAG 1 cut(s) 396
SinI GGWCC 1 cut(s) 367
SlaI CTCGAG 1 cut(s) 396
SmlI CTYRAG 3 cut(s) 224, 396, 437
SmoI CTYRAG 3 cut(s) 224, 396, 437
Sse9I AATT 4 cut(s) 15, 40, 64, 154
SsiI CCGC 3 cut(s) 267, 270, 313
SspDI GGCGCC 1 cut(s) 286
SspMI CTAG 2 cut(s) 102, 356
TaiI ACGT 1 cut(s) 518
TaqI TCGA 1 cut(s) 397
TasI AATT 4 cut(s) 15, 40, 64, 154
TatI WGTACW 1 cut(s) 463
TauI GCSGC 1 cut(s) 272
TfiI GAWTC 1 cut(s) 449
Tru1I TTAA 2 cut(s) 83, 200
Tru9I TTAA 2 cut(s) 83, 200
TseFI GTSAC 1 cut(s) 8
Tsp45I GTSAC 1 cut(s) 8
TspDTI ATGAA 1 cut(s) 33
Van91I CCANNNNNTGG 1 cut(s) 133
VpaK11BI GGWCC 1 cut(s) 367
XapI RAATTY 2 cut(s) 40, 64
XhoI CTCGAG 1 cut(s) 396
XmiI GTMKAC 1 cut(s) 115
XspI CTAG 2 cut(s) 102, 356
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.