MD14G1048200.v1.1

S-adenosyl-L-methionine-dependent protein-lysine N- methyltransferase that methylates elongation factor 1-alpha

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0012069
Physical Location & Seq
Reverse (-)
4601875 .. 4604280
2406 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1048200.v1.1.491

Sequence Viewer

Length: 726 bp
ATGGAACAAAACCTCTCCGCCCAAACCCTCCCCAACCACCGGAGCGTCGCCTCAAACGACGACGACGACGCACCGACCCTAAGCCTTCAAGCCCTAGAAGCTCTGAAGGAGTTCCTAGCCGAGCAAAGCCAGTCATCACCCGACACCGCCGCCGAGAGCCAAGCAACCGATTCGACCGGTCCGGCTCTGATCTCCGAAGACTGGAGACTGAGTCAGTTCTGGTACGACCCCCGCACCGCCCAAACCCTAGCCCAAGAGGTCCTCACTCTCTGCAACTCCGCCACCGCCTCCGCTCGGGTCGCCTGCGTGGCTTGCCCCTCACTCTACGCCTATCTCAAGAATGCTGATTCCGATGTGTCTGTACAACTGCTCGAGTATGACAAGCGGTTTGAGCAGTACGGCAGTGATTTCTCGTTCTACGATTACAACCGGCCTGAAGATTTGCCATTGGAGCTGAAGAATGCTTTTGACATTGTGGTTGCTGATCCTCCTTACCTGGAGTGTTTGGAGAAAGTTGCTCAAACAATTTCATTTCTAGCACGATCAGAAAAATCTCACTTGCTTCTCCTCACAGGAGCTGTGCAGAAAGAGAGAGCAGCTGAACTCATGGGATTGCATCCGTGTGGTTTTAGGCCTCAACACTCTAGCAAACTAGGAAACGAGTTTCGGCTGTTCACAAACTATGATCCTGAGACGAGATTAGAAGGGTGGGAGGTAGAGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
N6-adenineMlase

Protein Analysis

242

Amino Acids

26.97

Weight (kDa)

4.52

Isoelectric Point (pI)

49.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
N6-adenineMlase PF10237 66 - 229 2.2e-54 Probable N6-adenine methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012069)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G58470
fragaria_vesca FvH4_6g21270
malus_domestica MD14G1048200.v1.1
prunus_persica Prupe.7G087000_v2.0.a1
pyrus_communis pycom14g03970
rosa_chinensis RchiOBHm_Chr3g0477741 RchiOBHm_Chr3g0477781
rosa_laevigata RLG00000023692
rosa_multiflora Rmu_sc0017665.1_g000001 Rmu_sc0026290.1_g000001
rosa_roxburghii Rroxscaffold_6G00403710
rosa_rugosa Rorug03G0162900 Rorug03G0163000 Rorug03G0163100
rosa_samantha Rh3AG213400 Rh3BG247200 Rh3CG241000 Rh3DG240300
rosa_wichuraiana Rw3G019310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 293
AciI CCGC 9 cut(s) 18, 147, 150, 232, 237, 279, 285, 291, 385
AclWI GGATC 2 cut(s) 479, 680
AcuI CTGAAG 3 cut(s) 125, 456, 476
AfaI GTAC 3 cut(s) 224, 363, 398
AfiI CCNNNNNNNGG 3 cut(s) 39, 201, 294
AgeI ACCGGT 1 cut(s) 176
AgsI TTSAA 1 cut(s) 89
AjnI CCWGG 1 cut(s) 495
AluBI AGCT 4 cut(s) 101, 454, 578, 599
AluI AGCT 4 cut(s) 101, 454, 578, 599
Alw26I GTCTC 2 cut(s) 199, 686
AlwI GGATC 2 cut(s) 479, 680
AlwNI CAGNNNCTG 1 cut(s) 578
Ama87I CYCGRG 2 cut(s) 294, 371
AoxI GGCC 2 cut(s) 431, 632
ApeKI GCWGC 1 cut(s) 596
ArsI GACNNNNNNTTYG 2 cut(s) 371, 403
AsiGI ACCGGT 1 cut(s) 176
Asp700I GAANNNNTTC 1 cut(s) 110
AspS9I GGNCC 2 cut(s) 179, 259
AsuHPI GGTGA 1 cut(s) 129
AvaI CYCGRG 2 cut(s) 294, 371
AvaII GGWCC 2 cut(s) 179, 259
BbsI GAAGAC 1 cut(s) 204
BbvI GCAGC 1 cut(s) 608
BceAI ACGGC 1 cut(s) 415
BcgI CGANNNNNNTGC 2 cut(s) 153, 187
BciT130I CCWGG 1 cut(s) 497
BcoDI GTCTC 2 cut(s) 199, 686
BfaI CTAG 6 cut(s) 95, 116, 248, 536, 645, 653
BglI GCCNNNNNGGC 1 cut(s) 308
BisI GCNGC 2 cut(s) 150, 597
BlsI GCNGC 2 cut(s) 151, 598
Bme1390I CCNGG 1 cut(s) 497
Bme18I GGWCC 2 cut(s) 179, 259
BmeT110I CYCGRG 2 cut(s) 294, 371
BmgT120I GGNCC 2 cut(s) 179, 259
BmrFI CCNGG 1 cut(s) 497
BmsI GCATC 1 cut(s) 625
BpiI GAAGAC 1 cut(s) 204
BpmI CTGGAG 2 cut(s) 223, 518
Bpu10I CCTNAGC 1 cut(s) 80
BpuEI CTTGAG 1 cut(s) 320
BsaWI WCCGGW 2 cut(s) 39, 176
BsaXI ACNNNNNCTCC 2 cut(s) 196, 226
Bsc4I CCNNNNNNNGG 3 cut(s) 39, 201, 294
Bse118I RCCGGY 2 cut(s) 176, 429
Bse1I ACTGG 2 cut(s) 130, 206
BseBI CCWGG 1 cut(s) 497
BseGI GGATG 1 cut(s) 616
BseLI CCNNNNNNNGG 3 cut(s) 39, 201, 294
BseMII CTCAG 2 cut(s) 200, 681
BseNI ACTGG 2 cut(s) 130, 206
BseRI GAGGAG 1 cut(s) 557
BseXI GCAGC 1 cut(s) 608
BsgI GTGCAG 1 cut(s) 602
Bsh1285I CGRYCG 1 cut(s) 177
BshFI GGCC 2 cut(s) 433, 634
BshTI ACCGGT 1 cut(s) 176
BsiEI CGRYCG 1 cut(s) 177
BsiHKCI CYCGRG 2 cut(s) 294, 371
BsiSI CCGG 4 cut(s) 40, 177, 182, 430
BslI CCNNNNNNNGG 3 cut(s) 39, 201, 294
BsmAI GTCTC 2 cut(s) 199, 686
BsmBI CGTCTC 1 cut(s) 686
BsmI GAATGC 2 cut(s) 346, 466
BsnI GGCC 2 cut(s) 433, 634
BsoBI CYCGRG 2 cut(s) 294, 371
Bsp1407I TGTACA 1 cut(s) 361
Bsp143I GATC 4 cut(s) 189, 484, 542, 685
BspACI CCGC 9 cut(s) 18, 147, 150, 232, 237, 279, 285, 291, 385
BspANI GGCC 2 cut(s) 433, 634
BspCNI CTCAG 2 cut(s) 201, 682
BspPI GGATC 2 cut(s) 479, 680
BsrBI CCGCTC 1 cut(s) 293
BsrFI RCCGGY 2 cut(s) 176, 429
BsrGI TGTACA 1 cut(s) 361
BsrI ACTGG 2 cut(s) 130, 206
BssAI RCCGGY 2 cut(s) 176, 429
BssMI GATC 4 cut(s) 189, 484, 542, 685
Bst2UI CCWGG 1 cut(s) 497
BstAUI TGTACA 1 cut(s) 361
BstC8I GCNNGC 2 cut(s) 304, 313
BstDEI CTNAG 3 cut(s) 80, 209, 690
BstF5I GGATG 1 cut(s) 616
BstKTI GATC 4 cut(s) 192, 487, 545, 688
BstMAI GTCTC 2 cut(s) 199, 686
BstMBI GATC 4 cut(s) 189, 484, 542, 685
BstMCI CGRYCG 1 cut(s) 177
BstMWI GCNNNNNNNGC 6 cut(s) 98, 299, 308, 312, 391, 451
BstNI CCWGG 1 cut(s) 497
BstSCI CCNGG 1 cut(s) 495
BstV1I GCAGC 1 cut(s) 608
BstV2I GAAGAC 1 cut(s) 204
BsuRI GGCC 2 cut(s) 433, 634
BtsCI GGATG 1 cut(s) 616
BtsI GCAGTG 1 cut(s) 409
BtsIMutI CAGTG 1 cut(s) 409
Cac8I GCNNGC 2 cut(s) 304, 313
CaiI CAGNNNCTG 1 cut(s) 578
Cfr10I RCCGGY 2 cut(s) 176, 429
Cfr13I GGNCC 2 cut(s) 179, 259
CpoI CGGWCCG 1 cut(s) 179
CseI GACGC 2 cut(s) 34, 77
Csp6I GTAC 3 cut(s) 223, 362, 397
CspAI ACCGGT 1 cut(s) 176
CspI CGGWCCG 1 cut(s) 179
CviAII CATG 1 cut(s) 607
CviQI GTAC 3 cut(s) 223, 362, 397
DdeI CTNAG 3 cut(s) 80, 209, 690
DpnI GATC 4 cut(s) 191, 486, 544, 687
DpnII GATC 4 cut(s) 189, 484, 542, 685
EciI GGCGGA 2 cut(s) 7, 268
Eco147I AGGCCT 1 cut(s) 634
Eco47I GGWCC 2 cut(s) 179, 259
Eco57I CTGAAG 3 cut(s) 125, 456, 476
Eco88I CYCGRG 2 cut(s) 294, 371
EcoO109I RGGNCCY 1 cut(s) 259
EcoRII CCWGG 1 cut(s) 495
Esp3I CGTCTC 1 cut(s) 686
FaeI CATG 1 cut(s) 610
FaiI YATR 3 cut(s) 378, 608, 684
FatI CATG 1 cut(s) 606
FauI CCCGC 1 cut(s) 239
Fnu4HI GCNGC 2 cut(s) 150, 597
FokI GGATG 1 cut(s) 603
Fsp4HI GCNGC 2 cut(s) 150, 597
FspBI CTAG 6 cut(s) 95, 116, 248, 536, 645, 653
GluI GCNGC 2 cut(s) 150, 597
GsuI CTGGAG 2 cut(s) 223, 518
HaeIII GGCC 2 cut(s) 433, 634
HapII CCGG 4 cut(s) 40, 177, 182, 430
HgaI GACGC 2 cut(s) 34, 77
Hin1II CATG 1 cut(s) 610
HinfI GANTC 3 cut(s) 170, 211, 347
HpaII CCGG 4 cut(s) 40, 177, 182, 430
HphI GGTGA 1 cut(s) 129
Hpy166II GTNNAC 1 cut(s) 675
Hpy188I TCNGA 5 cut(s) 105, 189, 196, 352, 547
Hpy188III TCNNGA 2 cut(s) 337, 689
Hpy8I GTNNAC 1 cut(s) 675
Hpy99I CGWCG 5 cut(s) 50, 62, 65, 68, 71
HpyAV CCTTC 3 cut(s) 95, 100, 698
HpyCH4V TGCA 3 cut(s) 273, 583, 616
HpyF10VI GCNNNNNNNGC 6 cut(s) 98, 299, 308, 312, 391, 451
HpyF3I CTNAG 3 cut(s) 80, 209, 690
Hsp92II CATG 1 cut(s) 610
Kzo9I GATC 4 cut(s) 189, 484, 542, 685
LmnI GCTCC 3 cut(s) 42, 451, 575
Lsp1109I GCAGC 1 cut(s) 608
LweI GCATC 1 cut(s) 625
MaeI CTAG 6 cut(s) 95, 116, 248, 536, 645, 653
MalI GATC 4 cut(s) 191, 486, 544, 687
MbiI CCGCTC 1 cut(s) 293
MboI GATC 4 cut(s) 189, 484, 542, 685
MboII GAAGA 3 cut(s) 209, 449, 469
MluCI AATT 1 cut(s) 525
MlyI GAGTC 1 cut(s) 220
MroXI GAANNNNTTC 1 cut(s) 110
MslI CAYNNNNRTG 1 cut(s) 621
MspA1I CMGCKG 1 cut(s) 599
MspI CCGG 4 cut(s) 40, 177, 182, 430
MspR9I CCNGG 1 cut(s) 497
Mva1269I GAATGC 2 cut(s) 346, 466
MvaI CCWGG 1 cut(s) 497
MwoI GCNNNNNNNGC 6 cut(s) 98, 299, 308, 312, 391, 451
NdeII GATC 4 cut(s) 189, 484, 542, 685
NlaIII CATG 1 cut(s) 610
NmeAIII GCCGAG 2 cut(s) 145, 178
PaeR7I CTCGAG 1 cut(s) 371
PceI AGGCCT 1 cut(s) 634
PcsI WCGNNNNNNNCGW 2 cut(s) 54, 63
PctI GAATGC 2 cut(s) 346, 466
PdmI GAANNNNTTC 1 cut(s) 110
PfeI GAWTC 2 cut(s) 170, 347
PflFI GACNNNGTC 1 cut(s) 210
PinAI ACCGGT 1 cut(s) 176
PkrI GCNGC 2 cut(s) 151, 598
PleI GAGTC 1 cut(s) 219
PpsI GAGTC 1 cut(s) 219
PpuMI RGGWCCY 1 cut(s) 259
Psp5II RGGWCCY 1 cut(s) 259
Psp6I CCWGG 1 cut(s) 495
PspGI CCWGG 1 cut(s) 495
PspPI GGNCC 2 cut(s) 179, 259
PspPPI RGGWCCY 1 cut(s) 259
PspXI VCTCGAGB 1 cut(s) 371
PstNI CAGNNNCTG 1 cut(s) 578
PsyI GACNNNGTC 1 cut(s) 210
PvuII CAGCTG 1 cut(s) 599
RsaI GTAC 3 cut(s) 224, 363, 398
RsaNI GTAC 3 cut(s) 223, 362, 397
RseI CAYNNNNRTG 1 cut(s) 621
Rsr2I CGGWCCG 1 cut(s) 179
RsrII CGGWCCG 1 cut(s) 179
SatI GCNGC 2 cut(s) 150, 597
Sau3AI GATC 4 cut(s) 189, 484, 542, 685
Sau96I GGNCC 2 cut(s) 179, 259
SchI GAGTC 1 cut(s) 220
ScrFI CCNGG 1 cut(s) 497
SetI ASST 8 cut(s) 15, 103, 261, 456, 498, 580, 601, 717
SfaNI GCATC 1 cut(s) 625
Sfr274I CTCGAG 1 cut(s) 371
SinI GGWCC 2 cut(s) 179, 259
SlaI CTCGAG 1 cut(s) 371
SmiMI CAYNNNNRTG 1 cut(s) 621
SmlI CTYRAG 2 cut(s) 335, 371
SmoI CTYRAG 2 cut(s) 335, 371
Sse9I AATT 1 cut(s) 525
SseBI AGGCCT 1 cut(s) 634
SsiI CCGC 9 cut(s) 18, 147, 150, 232, 237, 279, 285, 291, 385
SspMI CTAG 6 cut(s) 95, 116, 248, 536, 645, 653
StuI AGGCCT 1 cut(s) 634
StyD4I CCNGG 1 cut(s) 495
TaqI TCGA 2 cut(s) 173, 372
TasI AATT 1 cut(s) 525
TatI WGTACW 1 cut(s) 361
TauI GCSGC 1 cut(s) 152
TfiI GAWTC 2 cut(s) 170, 347
TscAI CASTG 1 cut(s) 409
TseI GCWGC 1 cut(s) 596
TspDTI ATGAA 1 cut(s) 519
TspGWI ACGGA 1 cut(s) 609
TspRI CASTG 1 cut(s) 409
Tth111I GACNNNGTC 1 cut(s) 210
VpaK11BI GGWCC 2 cut(s) 179, 259
XhoI CTCGAG 1 cut(s) 371
XmnI GAANNNNTTC 1 cut(s) 110
XspI CTAG 6 cut(s) 95, 116, 248, 536, 645, 653
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.