Rmu_sc0026290.1_g000001

S-adenosyl-L-methionine-dependent protein-lysine N- methyltransferase that methylates elongation factor 1-alpha

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0026290.1
OG0012069
Physical Location & Seq
Reverse (-)
1 .. 884
884 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0026290.1_g000001.1.cds

Sequence Viewer

Length: 490 bp
atggaacaagacctcctccccaattcaatctccggccaccaaaacgacgatgacgacgcaccgactttaagccttcaagccttagaagctctcaaggagttcctcaccgagcaaagccagtcctcaaacgacaccgccgccgaagcagactcggcgccggctttgatctcggaggactggcggatgagccagttctggtacgaccaccacacggcccaaaccctagccgaagaggtcctcactctctgcaaatcggactccactcgagtcgcctgcgtcgcttgtccctcgctctacgcctatctcaagagtattgattctcatgtgtctgtacaactgctggagtatgatagaaggtttgagcaatacgggagtgatttcacgttttatgattacaaccaaccagaagacttgcctttggagctaaagcatggctttgatgttgtagttgctgatcctccttatctggtgagctacattatgcgcattc
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
N6-adenineMlase

Protein Analysis

163

Amino Acids

18.51

Weight (kDa)

4.19

Isoelectric Point (pI)

60.12

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012069)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G58470
fragaria_vesca FvH4_6g21270
malus_domestica MD14G1048200.v1.1
prunus_persica Prupe.7G087000_v2.0.a1
pyrus_communis pycom14g03970
rosa_chinensis RchiOBHm_Chr3g0477741 RchiOBHm_Chr3g0477781
rosa_laevigata RLG00000023692
rosa_multiflora Rmu_sc0017665.1_g000001 Rmu_sc0026290.1_g000001
rosa_roxburghii Rroxscaffold_6G00403710
rosa_rugosa Rorug03G0162900 Rorug03G0163000 Rorug03G0163100
rosa_samantha Rh3AG213400 Rh3BG247200 Rh3CG241000 Rh3DG240300
rosa_wichuraiana Rw3G019310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 485
AccB1I GGYRCC 1 cut(s) 154
AciI CCGC 3 cut(s) 135, 138, 181
AclWI GGATC 1 cut(s) 449
AcoI YGGCCR 1 cut(s) 34
AcyI GRCGYC 1 cut(s) 155
AfaI GTAC 2 cut(s) 200, 333
AfiI CCNNNNNNNGG 1 cut(s) 211
AgsI TTSAA 2 cut(s) 27, 77
AloI GAACNNNNNNTCC 1 cut(s) 29
AluBI AGCT 3 cut(s) 89, 424, 474
AluI AGCT 3 cut(s) 89, 424, 474
AlwI GGATC 1 cut(s) 449
Ama87I CYCGRG 1 cut(s) 264
AoxI GGCC 2 cut(s) 34, 213
AspLEI GCGC 2 cut(s) 157, 486
AspS9I GGNCC 2 cut(s) 214, 235
AsuHPI GGTGA 2 cut(s) 97, 481
AvaI CYCGRG 1 cut(s) 264
AvaII GGWCC 1 cut(s) 235
BanI GGYRCC 1 cut(s) 154
BbsI GAAGAC 1 cut(s) 414
BceAI ACGGC 1 cut(s) 228
BcgI CGANNNNNNTGC 2 cut(s) 255, 289
BfaI CTAG 1 cut(s) 224
BfoI RGCGCY 1 cut(s) 158
BisI GCNGC 1 cut(s) 138
BlsI GCNGC 1 cut(s) 139
Bme18I GGWCC 1 cut(s) 235
BmeT110I CYCGRG 1 cut(s) 264
BmgT120I GGNCC 2 cut(s) 214, 235
BmiI GGNNCC 1 cut(s) 156
BpiI GAAGAC 1 cut(s) 414
BpmI CTGGAG 1 cut(s) 362
BpuEI CTTGAG 2 cut(s) 77, 290
BsaHI GRCGYC 1 cut(s) 155
BsaXI ACNNNNNCTCC 1 cut(s) 27
Bsc4I CCNNNNNNNGG 1 cut(s) 211
Bse118I RCCGGY 1 cut(s) 157
Bse1I ACTGG 3 cut(s) 118, 182, 190
BseGI GGATG 1 cut(s) 189
BseLI CCNNNNNNNGG 1 cut(s) 211
BseNI ACTGG 3 cut(s) 118, 182, 190
BseRI GAGGAG 1 cut(s) 5
BshFI GGCC 2 cut(s) 36, 215
BshNI GGYRCC 1 cut(s) 154
BsiHKCI CYCGRG 1 cut(s) 264
BsiSI CCGG 2 cut(s) 33, 158
BslFI GGGAC 1 cut(s) 270
BslI CCNNNNNNNGG 1 cut(s) 211
BsmFI GGGAC 1 cut(s) 270
BsmI GAATGC 1 cut(s) 486
BsnI GGCC 2 cut(s) 36, 215
BsoBI CYCGRG 1 cut(s) 264
Bsp1407I TGTACA 1 cut(s) 331
Bsp143I GATC 2 cut(s) 165, 454
BspACI CCGC 3 cut(s) 135, 138, 181
BspANI GGCC 2 cut(s) 36, 215
BspLI GGNNCC 1 cut(s) 156
BspPI GGATC 1 cut(s) 449
BspT107I GGYRCC 1 cut(s) 154
BsrFI RCCGGY 1 cut(s) 157
BsrGI TGTACA 1 cut(s) 331
BsrI ACTGG 3 cut(s) 118, 182, 190
BssAI RCCGGY 1 cut(s) 157
BssMI GATC 2 cut(s) 165, 454
BssNI GRCGYC 1 cut(s) 155
Bst6I CTCTTC 1 cut(s) 225
BstACI GRCGYC 1 cut(s) 155
BstAUI TGTACA 1 cut(s) 331
BstC8I GCNNGC 2 cut(s) 159, 274
BstDEI CTNAG 1 cut(s) 82
BstF5I GGATG 1 cut(s) 189
BstH2I RGCGCY 1 cut(s) 158
BstHHI GCGC 2 cut(s) 157, 486
BstKTI GATC 2 cut(s) 168, 457
BstMBI GATC 2 cut(s) 165, 454
BstMWI GCNNNNNNNGC 5 cut(s) 86, 143, 152, 278, 421
BstV2I GAAGAC 1 cut(s) 414
BsuRI GGCC 2 cut(s) 36, 215
BtsCI GGATG 1 cut(s) 189
Cac8I GCNNGC 2 cut(s) 159, 274
CfoI GCGC 2 cut(s) 157, 486
Cfr10I RCCGGY 1 cut(s) 157
Cfr13I GGNCC 2 cut(s) 214, 235
CseI GACGC 2 cut(s) 65, 265
Csp6I GTAC 2 cut(s) 199, 332
CviAII CATG 2 cut(s) 323, 431
CviQI GTAC 2 cut(s) 199, 332
DdeI CTNAG 1 cut(s) 82
DinI GGCGCC 1 cut(s) 156
DpnI GATC 2 cut(s) 167, 456
DpnII GATC 2 cut(s) 165, 454
EaeI YGGCCR 1 cut(s) 34
Eam1104I CTCTTC 1 cut(s) 225
EarI CTCTTC 1 cut(s) 225
EciI GGCGGA 1 cut(s) 196
Eco47I GGWCC 1 cut(s) 235
Eco88I CYCGRG 1 cut(s) 264
EcoO109I RGGNCCY 1 cut(s) 235
EgeI GGCGCC 1 cut(s) 156
EheI GGCGCC 1 cut(s) 156
FaeI CATG 2 cut(s) 326, 434
FaiI YATR 5 cut(s) 324, 348, 390, 432, 482
FalI AAGNNNNNCTT 2 cut(s) 419, 451
FaqI GGGAC 1 cut(s) 270
FatI CATG 2 cut(s) 322, 430
Fnu4HI GCNGC 1 cut(s) 138
FokI GGATG 1 cut(s) 196
Fsp4HI GCNGC 1 cut(s) 138
FspAI RTGCGCAY 1 cut(s) 485
FspBI CTAG 1 cut(s) 224
FspI TGCGCA 1 cut(s) 485
GlaI GCGC 2 cut(s) 156, 485
GluI GCNGC 1 cut(s) 138
GsuI CTGGAG 1 cut(s) 362
HaeII RGCGCY 1 cut(s) 158
HaeIII GGCC 2 cut(s) 36, 215
HapII CCGG 2 cut(s) 33, 158
HgaI GACGC 2 cut(s) 65, 265
HhaI GCGC 2 cut(s) 157, 486
Hin1I GRCGYC 1 cut(s) 155
Hin1II CATG 2 cut(s) 326, 434
Hin6I GCGC 2 cut(s) 155, 484
HinP1I GCGC 2 cut(s) 155, 484
HinfI GANTC 4 cut(s) 149, 257, 267, 317
HpaII CCGG 2 cut(s) 33, 158
HphI GGTGA 2 cut(s) 97, 481
Hpy188I TCNGA 2 cut(s) 172, 256
Hpy188III TCNNGA 1 cut(s) 307
Hpy99I CGWCG 3 cut(s) 50, 59, 281
HpyAV CCTTC 2 cut(s) 83, 348
HpyCH4IV ACGT 1 cut(s) 383
HpyCH4V TGCA 1 cut(s) 249
HpyF10VI GCNNNNNNNGC 5 cut(s) 86, 143, 152, 278, 421
HpyF3I CTNAG 1 cut(s) 82
HpySE526I ACGT 1 cut(s) 383
Hsp92I GRCGYC 1 cut(s) 155
Hsp92II CATG 2 cut(s) 326, 434
HspAI GCGC 2 cut(s) 155, 484
KasI GGCGCC 1 cut(s) 154
KroI GCCGGC 1 cut(s) 157
KroNI GCCGGC 1 cut(s) 159
Kzo9I GATC 2 cut(s) 165, 454
LmnI GCTCC 1 cut(s) 421
MaeI CTAG 1 cut(s) 224
MaeII ACGT 1 cut(s) 383
MalI GATC 2 cut(s) 167, 456
MboI GATC 2 cut(s) 165, 454
MboII GAAGA 2 cut(s) 242, 419
MluCI AATT 1 cut(s) 22
Mly113I GGCGCC 1 cut(s) 155
MlyI GAGTC 3 cut(s) 143, 251, 276
MnlI CCTC 9 cut(s) 23, 26, 113, 133, 166, 226, 248, 298, 468
MroNI GCCGGC 1 cut(s) 157
MseI TTAA 1 cut(s) 68
MspI CCGG 2 cut(s) 33, 158
Mva1269I GAATGC 1 cut(s) 486
MwoI GCNNNNNNNGC 5 cut(s) 86, 143, 152, 278, 421
NaeI GCCGGC 1 cut(s) 159
NarI GGCGCC 1 cut(s) 155
NdeII GATC 2 cut(s) 165, 454
NgoMIV GCCGGC 1 cut(s) 157
NlaIII CATG 2 cut(s) 326, 434
NlaIV GGNNCC 1 cut(s) 156
NmeAIII GCCGAG 1 cut(s) 131
NsbI TGCGCA 1 cut(s) 485
PaeR7I CTCGAG 1 cut(s) 264
PcsI WCGNNNNNNNCGW 1 cut(s) 51
PctI GAATGC 1 cut(s) 486
PdiI GCCGGC 1 cut(s) 159
PfeI GAWTC 1 cut(s) 317
PkrI GCNGC 1 cut(s) 139
PleI GAGTC 3 cut(s) 143, 251, 275
PluTI GGCGCC 1 cut(s) 158
PpsI GAGTC 3 cut(s) 143, 251, 275
PpuMI RGGWCCY 1 cut(s) 235
Psp5II RGGWCCY 1 cut(s) 235
PspN4I GGNNCC 1 cut(s) 156
PspPI GGNCC 2 cut(s) 214, 235
PspPPI RGGWCCY 1 cut(s) 235
PspXI VCTCGAGB 1 cut(s) 264
RsaI GTAC 2 cut(s) 200, 333
RsaNI GTAC 2 cut(s) 199, 332
SaqAI TTAA 1 cut(s) 68
SatI GCNGC 1 cut(s) 138
Sau3AI GATC 2 cut(s) 165, 454
Sau96I GGNCC 2 cut(s) 214, 235
SchI GAGTC 3 cut(s) 143, 251, 276
SetI ASST 7 cut(s) 15, 91, 237, 359, 386, 426, 476
SfoI GGCGCC 1 cut(s) 156
Sfr274I CTCGAG 1 cut(s) 264
SinI GGWCC 1 cut(s) 235
SlaI CTCGAG 1 cut(s) 264
SmlI CTYRAG 3 cut(s) 92, 264, 305
SmoI CTYRAG 3 cut(s) 92, 264, 305
Sse9I AATT 1 cut(s) 22
SsiI CCGC 3 cut(s) 135, 138, 181
SspDI GGCGCC 1 cut(s) 154
SspMI CTAG 1 cut(s) 224
TaiI ACGT 1 cut(s) 386
TaqI TCGA 1 cut(s) 265
TasI AATT 1 cut(s) 22
TatI WGTACW 1 cut(s) 331
TauI GCSGC 1 cut(s) 140
TfiI GAWTC 1 cut(s) 317
Tru1I TTAA 1 cut(s) 68
Tru9I TTAA 1 cut(s) 68
VpaK11BI GGWCC 1 cut(s) 235
XhoI CTCGAG 1 cut(s) 264
XspI CTAG 1 cut(s) 224
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.