RLG00000023692

S-adenosyl-L-methionine-dependent protein-lysine N- methyltransferase that methylates elongation factor 1-alpha

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0012069
Physical Location & Seq
Forward (+)
27143968 .. 27146221
2254 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023692

Sequence Viewer

Length: 729 bp
ATGGAGGCAGACCCACACTTCTTCACTACTAGACACTTTGGTCTACACCGCCACCAACAAATGGAACAAGACCTCCTCCCCAATTCAATCTCCGGCCACCAAAACGACGATGACGACGCACCGACTCTAAGCCTTCAAGCCTTAGAAGCTCTCAAGGAGTTCCTCACCGAGCAAAGCCAGTCCTCAAACGACACCGCCGCCGAAGCAAACTCGGCGCCGGCTTTGATCTCGGAGGACTGGCGGATGAGCCAGTTCTGGTACGACCACCACACGGCCCAAACCCTAGCCGAAGAGGTCCTCACTCTCTGCAAATCGGACTCCACTCGAGTCGCCTGCGTCGCTTGTCCCTCGCTCTACGCCTATCTCAAGAGTATTGATTCTCATGTGTCTGTACAACTGCTGGAGTATGATAGAAGGTTTGAGCAATACGGGAGTGATTTCACGTTTTATGATTACAACCAACCAGAAGACTTGCCTTTGGAGCTGAAGCATGGTTTTGATATTGTAGTTGCTGATCCTCCTTATCTGAGTCAAGAGTGCTTGGAGAAAGTCGCTAAAACTGTTTCATTTCTTGCACGATCAGAAAAACCTTACTTGCTCCTCCTCACAGGAGAGGTGCAGAAAGAGAGAGCAGCTGAGCTCATGGGATTGCTTCCATATTGGGAGGGTGGGACAATTAGGAACTCTATTCTCATGTGCCTGACACTGATTCAACAGCTACGCAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
N6-adenineMlase

Protein Analysis

243

Amino Acids

27.61

Weight (kDa)

4.7

Isoelectric Point (pI)

54.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
N6-adenineMlase PF10237 78 - 217 4.4e-48 Probable N6-adenine methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012069)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G58470
fragaria_vesca FvH4_6g21270
malus_domestica MD14G1048200.v1.1
prunus_persica Prupe.7G087000_v2.0.a1
pyrus_communis pycom14g03970
rosa_chinensis RchiOBHm_Chr3g0477741 RchiOBHm_Chr3g0477781
rosa_laevigata RLG00000023692
rosa_multiflora Rmu_sc0017665.1_g000001 Rmu_sc0026290.1_g000001
rosa_roxburghii Rroxscaffold_6G00403710
rosa_rugosa Rorug03G0162900 Rorug03G0163000 Rorug03G0163100
rosa_samantha Rh3AG213400 Rh3BG247200 Rh3CG241000 Rh3DG240300
rosa_wichuraiana Rw3G019310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 39
AccB1I GGYRCC 1 cut(s) 214
AccB7I CCANNNNNTGG 1 cut(s) 61
AccI GTMKAC 1 cut(s) 43
AciI CCGC 4 cut(s) 49, 195, 198, 241
AclWI GGATC 1 cut(s) 509
AcoI YGGCCR 1 cut(s) 94
AcuI CTGAAG 1 cut(s) 506
AcyI GRCGYC 1 cut(s) 215
AfaI GTAC 2 cut(s) 260, 393
AfiI CCNNNNNNNGG 2 cut(s) 61, 271
AgsI TTSAA 3 cut(s) 87, 137, 713
AloI GAACNNNNNNTCC 2 cut(s) 57, 89
AluBI AGCT 5 cut(s) 149, 484, 635, 640, 718
AluI AGCT 5 cut(s) 149, 484, 635, 640, 718
Alw21I GWGCWC 1 cut(s) 642
AlwI GGATC 1 cut(s) 509
Ama87I CYCGRG 1 cut(s) 324
AoxI GGCC 2 cut(s) 94, 273
ApeKI GCWGC 1 cut(s) 632
AspLEI GCGC 1 cut(s) 217
AspS9I GGNCC 2 cut(s) 274, 295
AsuHPI GGTGA 1 cut(s) 157
AvaI CYCGRG 1 cut(s) 324
AvaII GGWCC 1 cut(s) 295
BanI GGYRCC 1 cut(s) 214
BanII GRGCYC 1 cut(s) 642
BbsI GAAGAC 1 cut(s) 474
Bbv12I GWGCWC 1 cut(s) 642
BbvI GCAGC 1 cut(s) 644
BceAI ACGGC 1 cut(s) 288
BcgI CGANNNNNNTGC 2 cut(s) 315, 349
BfaI CTAG 2 cut(s) 30, 284
BfoI RGCGCY 1 cut(s) 218
BisI GCNGC 2 cut(s) 198, 633
BlpI GCTNAGC 1 cut(s) 636
BlsI GCNGC 2 cut(s) 199, 634
Bme18I GGWCC 1 cut(s) 295
BmeT110I CYCGRG 1 cut(s) 324
BmgT120I GGNCC 2 cut(s) 274, 295
BmiI GGNNCC 1 cut(s) 216
BpiI GAAGAC 1 cut(s) 474
BpmI CTGGAG 1 cut(s) 422
Bpu1102I GCTNAGC 1 cut(s) 636
BpuEI CTTGAG 2 cut(s) 137, 350
BsaHI GRCGYC 1 cut(s) 215
BsaXI ACNNNNNCTCC 2 cut(s) 57, 87
Bsc4I CCNNNNNNNGG 2 cut(s) 61, 271
Bse118I RCCGGY 1 cut(s) 217
Bse1I ACTGG 3 cut(s) 178, 242, 250
BseGI GGATG 1 cut(s) 249
BseLI CCNNNNNNNGG 2 cut(s) 61, 271
BseMII CTCAG 2 cut(s) 518, 627
BseNI ACTGG 3 cut(s) 178, 242, 250
BseRI GAGGAG 3 cut(s) 65, 590, 593
BseXI GCAGC 1 cut(s) 644
BsgI GTGCAG 1 cut(s) 638
BshFI GGCC 2 cut(s) 96, 275
BshNI GGYRCC 1 cut(s) 214
BsiHKAI GWGCWC 1 cut(s) 642
BsiHKCI CYCGRG 1 cut(s) 324
BsiSI CCGG 2 cut(s) 93, 218
BslFI GGGAC 2 cut(s) 330, 685
BslI CCNNNNNNNGG 2 cut(s) 61, 271
BsmFI GGGAC 2 cut(s) 330, 685
BsnI GGCC 2 cut(s) 96, 275
BsoBI CYCGRG 1 cut(s) 324
Bsp1286I GDGCHC 1 cut(s) 642
Bsp1407I TGTACA 1 cut(s) 391
Bsp143I GATC 3 cut(s) 225, 514, 578
Bsp1720I GCTNAGC 1 cut(s) 636
BspACI CCGC 4 cut(s) 49, 195, 198, 241
BspANI GGCC 2 cut(s) 96, 275
BspCNI CTCAG 2 cut(s) 519, 628
BspLI GGNNCC 1 cut(s) 216
BspPI GGATC 1 cut(s) 509
BspT107I GGYRCC 1 cut(s) 214
BsrFI RCCGGY 1 cut(s) 217
BsrGI TGTACA 1 cut(s) 391
BsrI ACTGG 3 cut(s) 178, 242, 250
BssAI RCCGGY 1 cut(s) 217
BssMI GATC 3 cut(s) 225, 514, 578
BssNI GRCGYC 1 cut(s) 215
Bst4CI ACNGT 1 cut(s) 562
Bst6I CTCTTC 1 cut(s) 285
BstACI GRCGYC 1 cut(s) 215
BstAUI TGTACA 1 cut(s) 391
BstC8I GCNNGC 2 cut(s) 219, 334
BstDEI CTNAG 4 cut(s) 128, 142, 527, 636
BstF5I GGATG 1 cut(s) 249
BstH2I RGCGCY 1 cut(s) 218
BstHHI GCGC 1 cut(s) 217
BstKTI GATC 3 cut(s) 228, 517, 581
BstMBI GATC 3 cut(s) 225, 514, 578
BstMWI GCNNNNNNNGC 5 cut(s) 146, 203, 212, 338, 481
BstV1I GCAGC 1 cut(s) 644
BstV2I GAAGAC 1 cut(s) 474
BsuRI GGCC 2 cut(s) 96, 275
BtsCI GGATG 1 cut(s) 249
BtsIMutI CAGTG 1 cut(s) 704
Cac8I GCNNGC 2 cut(s) 219, 334
CfoI GCGC 1 cut(s) 217
Cfr10I RCCGGY 1 cut(s) 217
Cfr13I GGNCC 2 cut(s) 274, 295
CseI GACGC 2 cut(s) 125, 325
Csp6I GTAC 2 cut(s) 259, 392
CviAII CATG 4 cut(s) 383, 491, 643, 694
CviQI GTAC 2 cut(s) 259, 392
DdeI CTNAG 4 cut(s) 128, 142, 527, 636
DinI GGCGCC 1 cut(s) 216
DpnI GATC 3 cut(s) 227, 516, 580
DpnII GATC 3 cut(s) 225, 514, 578
DrdI GACNNNNNNGTC 1 cut(s) 39
DseDI GACNNNNNNGTC 1 cut(s) 39
EaeI YGGCCR 1 cut(s) 94
Eam1104I CTCTTC 1 cut(s) 285
EarI CTCTTC 1 cut(s) 285
EciI GGCGGA 1 cut(s) 256
Ecl136II GAGCTC 1 cut(s) 640
Eco24I GRGCYC 1 cut(s) 642
Eco47I GGWCC 1 cut(s) 295
Eco53kI GAGCTC 1 cut(s) 640
Eco57I CTGAAG 1 cut(s) 506
Eco88I CYCGRG 1 cut(s) 324
EcoICRI GAGCTC 1 cut(s) 640
EcoO109I RGGNCCY 1 cut(s) 295
EcoT38I GRGCYC 1 cut(s) 642
EgeI GGCGCC 1 cut(s) 216
EheI GGCGCC 1 cut(s) 216
FaeI CATG 4 cut(s) 386, 494, 646, 697
FaiI YATR 7 cut(s) 384, 408, 450, 492, 644, 658, 695
FaqI GGGAC 2 cut(s) 330, 685
FatI CATG 4 cut(s) 382, 490, 642, 693
FblI GTMKAC 1 cut(s) 43
Fnu4HI GCNGC 2 cut(s) 198, 633
FokI GGATG 1 cut(s) 256
FriOI GRGCYC 1 cut(s) 642
Fsp4HI GCNGC 2 cut(s) 198, 633
FspBI CTAG 2 cut(s) 30, 284
GlaI GCGC 1 cut(s) 216
GluI GCNGC 2 cut(s) 198, 633
GsuI CTGGAG 1 cut(s) 422
HaeII RGCGCY 1 cut(s) 218
HaeIII GGCC 2 cut(s) 96, 275
HapII CCGG 2 cut(s) 93, 218
HgaI GACGC 2 cut(s) 125, 325
HhaI GCGC 1 cut(s) 217
Hin1I GRCGYC 1 cut(s) 215
Hin1II CATG 4 cut(s) 386, 494, 646, 697
Hin6I GCGC 1 cut(s) 215
HinP1I GCGC 1 cut(s) 215
HinfI GANTC 6 cut(s) 124, 317, 327, 377, 529, 709
HpaII CCGG 2 cut(s) 93, 218
HphI GGTGA 1 cut(s) 157
Hpy166II GTNNAC 1 cut(s) 44
Hpy188I TCNGA 4 cut(s) 232, 316, 528, 583
Hpy188III TCNNGA 2 cut(s) 367, 533
Hpy8I GTNNAC 1 cut(s) 44
Hpy99I CGWCG 3 cut(s) 110, 119, 341
HpyAV CCTTC 2 cut(s) 143, 408
HpyCH4III ACNGT 1 cut(s) 562
HpyCH4IV ACGT 1 cut(s) 443
HpyCH4V TGCA 3 cut(s) 309, 575, 619
HpyF10VI GCNNNNNNNGC 5 cut(s) 146, 203, 212, 338, 481
HpyF3I CTNAG 4 cut(s) 128, 142, 527, 636
HpySE526I ACGT 1 cut(s) 443
Hsp92I GRCGYC 1 cut(s) 215
Hsp92II CATG 4 cut(s) 386, 494, 646, 697
HspAI GCGC 1 cut(s) 215
KasI GGCGCC 1 cut(s) 214
KroI GCCGGC 1 cut(s) 217
KroNI GCCGGC 1 cut(s) 219
Kzo9I GATC 3 cut(s) 225, 514, 578
LmnI GCTCC 2 cut(s) 481, 603
Lsp1109I GCAGC 1 cut(s) 644
MaeI CTAG 2 cut(s) 30, 284
MaeII ACGT 1 cut(s) 443
MalI GATC 3 cut(s) 227, 516, 580
MboI GATC 3 cut(s) 225, 514, 578
MboII GAAGA 3 cut(s) 13, 302, 479
MfeI CAATTG 1 cut(s) 724
MhlI GDGCHC 1 cut(s) 642
MluCI AATT 3 cut(s) 82, 675, 724
Mly113I GGCGCC 1 cut(s) 215
MlyI GAGTC 4 cut(s) 118, 311, 336, 538
MroNI GCCGGC 1 cut(s) 217
MspA1I CMGCKG 1 cut(s) 635
MspI CCGG 2 cut(s) 93, 218
MunI CAATTG 1 cut(s) 724
MwoI GCNNNNNNNGC 5 cut(s) 146, 203, 212, 338, 481
NaeI GCCGGC 1 cut(s) 219
NarI GGCGCC 1 cut(s) 215
NdeII GATC 3 cut(s) 225, 514, 578
NgoMIV GCCGGC 1 cut(s) 217
NlaIII CATG 4 cut(s) 386, 494, 646, 697
NlaIV GGNNCC 1 cut(s) 216
NmeAIII GCCGAG 1 cut(s) 191
PaeR7I CTCGAG 1 cut(s) 324
PcsI WCGNNNNNNNCGW 1 cut(s) 111
PdiI GCCGGC 1 cut(s) 219
PfeI GAWTC 2 cut(s) 377, 709
PflMI CCANNNNNTGG 1 cut(s) 61
PkrI GCNGC 2 cut(s) 199, 634
PleI GAGTC 4 cut(s) 118, 311, 335, 537
PluTI GGCGCC 1 cut(s) 218
PpsI GAGTC 4 cut(s) 118, 311, 335, 537
PpuMI RGGWCCY 1 cut(s) 295
Psp124BI GAGCTC 1 cut(s) 642
Psp5II RGGWCCY 1 cut(s) 295
PspN4I GGNNCC 1 cut(s) 216
PspPI GGNCC 2 cut(s) 274, 295
PspPPI RGGWCCY 1 cut(s) 295
PspXI VCTCGAGB 1 cut(s) 324
PvuII CAGCTG 1 cut(s) 635
RsaI GTAC 2 cut(s) 260, 393
RsaNI GTAC 2 cut(s) 259, 392
SacI GAGCTC 1 cut(s) 642
SatI GCNGC 2 cut(s) 198, 633
Sau3AI GATC 3 cut(s) 225, 514, 578
Sau96I GGNCC 2 cut(s) 274, 295
SchI GAGTC 4 cut(s) 118, 311, 336, 538
SduI GDGCHC 1 cut(s) 642
SfoI GGCGCC 1 cut(s) 216
Sfr274I CTCGAG 1 cut(s) 324
SinI GGWCC 1 cut(s) 295
SlaI CTCGAG 1 cut(s) 324
SmlI CTYRAG 3 cut(s) 152, 324, 365
SmoI CTYRAG 3 cut(s) 152, 324, 365
Sse9I AATT 3 cut(s) 82, 675, 724
SsiI CCGC 4 cut(s) 49, 195, 198, 241
SspDI GGCGCC 1 cut(s) 214
SspMI CTAG 2 cut(s) 30, 284
SstI GAGCTC 1 cut(s) 642
TaaI ACNGT 1 cut(s) 562
TaiI ACGT 1 cut(s) 446
TaqI TCGA 1 cut(s) 325
TasI AATT 3 cut(s) 82, 675, 724
TatI WGTACW 1 cut(s) 391
TauI GCSGC 1 cut(s) 200
TfiI GAWTC 2 cut(s) 377, 709
TscAI CASTG 1 cut(s) 711
TseI GCWGC 1 cut(s) 632
TspDTI ATGAA 1 cut(s) 555
TspRI CASTG 1 cut(s) 711
Van91I CCANNNNNTGG 1 cut(s) 61
VpaK11BI GGWCC 1 cut(s) 295
XhoI CTCGAG 1 cut(s) 324
XmiI GTMKAC 1 cut(s) 43
XspI CTAG 2 cut(s) 30, 284
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.