Rroxscaffold_6G00403710

S-adenosyl-L-methionine-dependent protein-lysine N- methyltransferase that methylates elongation factor 1-alpha

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0012069
Physical Location & Seq
Forward (+)
26185327 .. 26188184
2858 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00403710.1

Sequence Viewer

Length: 696 bp
ATGGAACAAGACCTCCTCCCCGATTCAATGTCCGGCCACCAAAACGACGATGACGACGCACCGACTCTAAGCCTTCAAGCCTTAGAAGCTCTCAAGGAGTTCCTCACCGAGCAAAGCCAGTCCTCAAACGACACCGCCGCCGAAGCAGACTCGGCGCCGGCTTTGATCTCGGAGGACTGGCGGATGAGCCAGTTCTGGTACGACCACCACACGGCCCAAACCCTAGCCGAAGAGGTCCTCACTCTCTGCAAATCGGACTCCACTCGAGTCGCCTGCGTCGCTTGTCCCTCGCTCTACGCCTATCTCAAGAGTATTGATTCTCATGTGTCTGTACAACTGCTGGAGTATGATAGAAGGTTTGAGCAATACGGGAGTGATTTCACGTTTTATGATTACAACCAACCAGAAGACCTGCCTTTGGAGCTAAAGCATGGCTTTGATGTTGTAGTTGCCTCCTTATCTGATCCTCCTTATCTGTGCTTGGAGAAAGTCGCTAAAACTGTTTCATTTCTTGCACGATCAGAAAAACCTTACTTGCTCCTCCTCACAGGAGAGGTGCAGAAAGAGAGAGCAGCTGAGCTCATGGGATTGCTTCCATGTGGTTTTAGGCCTCGGCACTCCAGCAAACTAGGAAATGAGTTTCGGCTGTTCACAAGTTATGATCCTGGGACTAGATTAGGAGGGTGGGACAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
N6-adenineMlase

Protein Analysis

231

Amino Acids

26.03

Weight (kDa)

4.53

Isoelectric Point (pI)

54.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
N6-adenineMlase PF10237 58 - 221 3.6e-49 Probable N6-adenine methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012069)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G58470
fragaria_vesca FvH4_6g21270
malus_domestica MD14G1048200.v1.1
prunus_persica Prupe.7G087000_v2.0.a1
pyrus_communis pycom14g03970
rosa_chinensis RchiOBHm_Chr3g0477741 RchiOBHm_Chr3g0477781
rosa_laevigata RLG00000023692
rosa_multiflora Rmu_sc0017665.1_g000001 Rmu_sc0026290.1_g000001
rosa_roxburghii Rroxscaffold_6G00403710
rosa_rugosa Rorug03G0162900 Rorug03G0163000 Rorug03G0163100
rosa_samantha Rh3AG213400 Rh3BG247200 Rh3CG241000 Rh3DG240300
rosa_wichuraiana Rw3G019310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 420
AccB1I GGYRCC 1 cut(s) 154
AciI CCGC 3 cut(s) 135, 138, 181
AclWI GGATC 2 cut(s) 458, 656
AcoI YGGCCR 1 cut(s) 34
AcyI GRCGYC 1 cut(s) 155
AfaI GTAC 2 cut(s) 200, 333
AfiI CCNNNNNNNGG 2 cut(s) 211, 418
AgsI TTSAA 2 cut(s) 27, 77
AjnI CCWGG 1 cut(s) 664
AloI GAACNNNNNNTCC 1 cut(s) 29
AluBI AGCT 4 cut(s) 89, 424, 575, 580
AluI AGCT 4 cut(s) 89, 424, 575, 580
Alw21I GWGCWC 1 cut(s) 582
AlwI GGATC 2 cut(s) 458, 656
Ama87I CYCGRG 1 cut(s) 264
AoxI GGCC 3 cut(s) 34, 213, 608
ApeKI GCWGC 1 cut(s) 572
AspLEI GCGC 1 cut(s) 157
AspS9I GGNCC 2 cut(s) 214, 235
AsuHPI GGTGA 1 cut(s) 97
AvaI CYCGRG 1 cut(s) 264
AvaII GGWCC 1 cut(s) 235
BanI GGYRCC 1 cut(s) 154
BanII GRGCYC 1 cut(s) 582
BbsI GAAGAC 1 cut(s) 414
Bbv12I GWGCWC 1 cut(s) 582
BbvI GCAGC 1 cut(s) 584
BceAI ACGGC 1 cut(s) 228
BcgI CGANNNNNNTGC 2 cut(s) 255, 289
BciT130I CCWGG 1 cut(s) 666
BfaI CTAG 3 cut(s) 224, 629, 672
BfoI RGCGCY 1 cut(s) 158
BfuAI ACCTGC 1 cut(s) 420
BisI GCNGC 2 cut(s) 138, 573
BlpI GCTNAGC 1 cut(s) 576
BlsI GCNGC 2 cut(s) 139, 574
Bme1390I CCNGG 1 cut(s) 666
Bme18I GGWCC 1 cut(s) 235
BmeT110I CYCGRG 1 cut(s) 264
BmgT120I GGNCC 2 cut(s) 214, 235
BmiI GGNNCC 1 cut(s) 156
BmrFI CCNGG 1 cut(s) 666
BpiI GAAGAC 1 cut(s) 414
BpmI CTGGAG 2 cut(s) 362, 604
Bpu1102I GCTNAGC 1 cut(s) 576
BpuEI CTTGAG 2 cut(s) 77, 290
BsaHI GRCGYC 1 cut(s) 155
BsaJI CCNNGG 2 cut(s) 611, 665
BsaXI ACNNNNNCTCC 1 cut(s) 27
Bsc4I CCNNNNNNNGG 2 cut(s) 211, 418
Bse118I RCCGGY 1 cut(s) 157
Bse1I ACTGG 3 cut(s) 118, 182, 190
BseBI CCWGG 1 cut(s) 666
BseDI CCNNGG 2 cut(s) 611, 665
BseGI GGATG 1 cut(s) 189
BseLI CCNNNNNNNGG 2 cut(s) 211, 418
BseMII CTCAG 1 cut(s) 567
BseNI ACTGG 3 cut(s) 118, 182, 190
BseRI GAGGAG 3 cut(s) 5, 530, 533
BseXI GCAGC 1 cut(s) 584
BsgI GTGCAG 1 cut(s) 578
BshFI GGCC 3 cut(s) 36, 215, 610
BshNI GGYRCC 1 cut(s) 154
BsiHKAI GWGCWC 1 cut(s) 582
BsiHKCI CYCGRG 1 cut(s) 264
BsiSI CCGG 2 cut(s) 33, 158
BslFI GGGAC 2 cut(s) 270, 682
BslI CCNNNNNNNGG 2 cut(s) 211, 418
BsmFI GGGAC 2 cut(s) 270, 682
BsnI GGCC 3 cut(s) 36, 215, 610
BsoBI CYCGRG 1 cut(s) 264
Bsp1286I GDGCHC 1 cut(s) 582
Bsp1407I TGTACA 1 cut(s) 331
Bsp143I GATC 4 cut(s) 165, 463, 518, 661
Bsp1720I GCTNAGC 1 cut(s) 576
BspACI CCGC 3 cut(s) 135, 138, 181
BspANI GGCC 3 cut(s) 36, 215, 610
BspCNI CTCAG 1 cut(s) 568
BspLI GGNNCC 1 cut(s) 156
BspMI ACCTGC 1 cut(s) 420
BspPI GGATC 2 cut(s) 458, 656
BspT107I GGYRCC 1 cut(s) 154
BsrFI RCCGGY 1 cut(s) 157
BsrGI TGTACA 1 cut(s) 331
BsrI ACTGG 3 cut(s) 118, 182, 190
BssAI RCCGGY 1 cut(s) 157
BssECI CCNNGG 2 cut(s) 611, 665
BssMI GATC 4 cut(s) 165, 463, 518, 661
BssNI GRCGYC 1 cut(s) 155
Bst2UI CCWGG 1 cut(s) 666
Bst4CI ACNGT 1 cut(s) 502
Bst6I CTCTTC 1 cut(s) 225
BstACI GRCGYC 1 cut(s) 155
BstAUI TGTACA 1 cut(s) 331
BstC8I GCNNGC 2 cut(s) 159, 274
BstDEI CTNAG 3 cut(s) 68, 82, 576
BstF5I GGATG 1 cut(s) 189
BstH2I RGCGCY 1 cut(s) 158
BstHHI GCGC 1 cut(s) 157
BstKTI GATC 4 cut(s) 168, 466, 521, 664
BstMBI GATC 4 cut(s) 165, 463, 518, 661
BstMWI GCNNNNNNNGC 5 cut(s) 86, 143, 152, 278, 421
BstNI CCWGG 1 cut(s) 666
BstSCI CCNGG 1 cut(s) 664
BstV1I GCAGC 1 cut(s) 584
BstV2I GAAGAC 1 cut(s) 414
BsuRI GGCC 3 cut(s) 36, 215, 610
BtsCI GGATG 1 cut(s) 189
BveI ACCTGC 1 cut(s) 420
Cac8I GCNNGC 2 cut(s) 159, 274
CfoI GCGC 1 cut(s) 157
Cfr10I RCCGGY 1 cut(s) 157
Cfr13I GGNCC 2 cut(s) 214, 235
CseI GACGC 2 cut(s) 65, 265
Csp6I GTAC 2 cut(s) 199, 332
CviAII CATG 4 cut(s) 323, 431, 583, 597
CviQI GTAC 2 cut(s) 199, 332
DdeI CTNAG 3 cut(s) 68, 82, 576
DinI GGCGCC 1 cut(s) 156
DpnI GATC 4 cut(s) 167, 465, 520, 663
DpnII GATC 4 cut(s) 165, 463, 518, 661
EaeI YGGCCR 1 cut(s) 34
Eam1104I CTCTTC 1 cut(s) 225
EarI CTCTTC 1 cut(s) 225
EciI GGCGGA 1 cut(s) 196
Ecl136II GAGCTC 1 cut(s) 580
Eco147I AGGCCT 1 cut(s) 610
Eco24I GRGCYC 1 cut(s) 582
Eco47I GGWCC 1 cut(s) 235
Eco53kI GAGCTC 1 cut(s) 580
Eco88I CYCGRG 1 cut(s) 264
EcoICRI GAGCTC 1 cut(s) 580
EcoO109I RGGNCCY 1 cut(s) 235
EcoRII CCWGG 1 cut(s) 664
EcoT38I GRGCYC 1 cut(s) 582
EgeI GGCGCC 1 cut(s) 156
EheI GGCGCC 1 cut(s) 156
FaeI CATG 4 cut(s) 326, 434, 586, 600
FaiI YATR 7 cut(s) 324, 348, 390, 432, 584, 598, 660
FalI AAGNNNNNCTT 2 cut(s) 419, 451
FaqI GGGAC 2 cut(s) 270, 682
FatI CATG 4 cut(s) 322, 430, 582, 596
Fnu4HI GCNGC 2 cut(s) 138, 573
FokI GGATG 1 cut(s) 196
FriOI GRGCYC 1 cut(s) 582
Fsp4HI GCNGC 2 cut(s) 138, 573
FspBI CTAG 3 cut(s) 224, 629, 672
GlaI GCGC 1 cut(s) 156
GluI GCNGC 2 cut(s) 138, 573
GsuI CTGGAG 2 cut(s) 362, 604
HaeII RGCGCY 1 cut(s) 158
HaeIII GGCC 3 cut(s) 36, 215, 610
HapII CCGG 2 cut(s) 33, 158
HgaI GACGC 2 cut(s) 65, 265
HhaI GCGC 1 cut(s) 157
Hin1I GRCGYC 1 cut(s) 155
Hin1II CATG 4 cut(s) 326, 434, 586, 600
Hin6I GCGC 1 cut(s) 155
HinP1I GCGC 1 cut(s) 155
HinfI GANTC 6 cut(s) 23, 64, 149, 257, 267, 317
HpaII CCGG 2 cut(s) 33, 158
HphI GGTGA 1 cut(s) 97
Hpy166II GTNNAC 1 cut(s) 651
Hpy188I TCNGA 4 cut(s) 172, 256, 463, 523
Hpy188III TCNNGA 1 cut(s) 307
Hpy8I GTNNAC 1 cut(s) 651
Hpy99I CGWCG 3 cut(s) 50, 59, 281
HpyAV CCTTC 2 cut(s) 83, 348
HpyCH4III ACNGT 1 cut(s) 502
HpyCH4IV ACGT 1 cut(s) 383
HpyCH4V TGCA 3 cut(s) 249, 515, 559
HpyF10VI GCNNNNNNNGC 5 cut(s) 86, 143, 152, 278, 421
HpyF3I CTNAG 3 cut(s) 68, 82, 576
HpySE526I ACGT 1 cut(s) 383
Hsp92I GRCGYC 1 cut(s) 155
Hsp92II CATG 4 cut(s) 326, 434, 586, 600
HspAI GCGC 1 cut(s) 155
KasI GGCGCC 1 cut(s) 154
KroI GCCGGC 1 cut(s) 157
KroNI GCCGGC 1 cut(s) 159
Kzo9I GATC 4 cut(s) 165, 463, 518, 661
LmnI GCTCC 2 cut(s) 421, 543
Lsp1109I GCAGC 1 cut(s) 584
MaeI CTAG 3 cut(s) 224, 629, 672
MaeII ACGT 1 cut(s) 383
MalI GATC 4 cut(s) 167, 465, 520, 663
MboI GATC 4 cut(s) 165, 463, 518, 661
MboII GAAGA 2 cut(s) 242, 419
MhlI GDGCHC 1 cut(s) 582
MluCI AATT 1 cut(s) 691
Mly113I GGCGCC 1 cut(s) 155
MlyI GAGTC 4 cut(s) 58, 143, 251, 276
MroNI GCCGGC 1 cut(s) 157
MspA1I CMGCKG 1 cut(s) 575
MspI CCGG 2 cut(s) 33, 158
MspR9I CCNGG 1 cut(s) 666
MvaI CCWGG 1 cut(s) 666
MwoI GCNNNNNNNGC 5 cut(s) 86, 143, 152, 278, 421
NaeI GCCGGC 1 cut(s) 159
NarI GGCGCC 1 cut(s) 155
NdeII GATC 4 cut(s) 165, 463, 518, 661
NgoMIV GCCGGC 1 cut(s) 157
NlaIII CATG 4 cut(s) 326, 434, 586, 600
NlaIV GGNNCC 1 cut(s) 156
NmeAIII GCCGAG 2 cut(s) 131, 592
PaeR7I CTCGAG 1 cut(s) 264
PceI AGGCCT 1 cut(s) 610
PcsI WCGNNNNNNNCGW 1 cut(s) 51
PdiI GCCGGC 1 cut(s) 159
PfeI GAWTC 2 cut(s) 23, 317
PkrI GCNGC 2 cut(s) 139, 574
PleI GAGTC 4 cut(s) 58, 143, 251, 275
PluTI GGCGCC 1 cut(s) 158
PpsI GAGTC 4 cut(s) 58, 143, 251, 275
PpuMI RGGWCCY 1 cut(s) 235
Psp124BI GAGCTC 1 cut(s) 582
Psp5II RGGWCCY 1 cut(s) 235
Psp6I CCWGG 1 cut(s) 664
PspGI CCWGG 1 cut(s) 664
PspN4I GGNNCC 1 cut(s) 156
PspPI GGNCC 2 cut(s) 214, 235
PspPPI RGGWCCY 1 cut(s) 235
PspXI VCTCGAGB 1 cut(s) 264
PvuII CAGCTG 1 cut(s) 575
RsaI GTAC 2 cut(s) 200, 333
RsaNI GTAC 2 cut(s) 199, 332
SacI GAGCTC 1 cut(s) 582
SatI GCNGC 2 cut(s) 138, 573
Sau3AI GATC 4 cut(s) 165, 463, 518, 661
Sau96I GGNCC 2 cut(s) 214, 235
SchI GAGTC 4 cut(s) 58, 143, 251, 276
ScrFI CCNGG 1 cut(s) 666
SduI GDGCHC 1 cut(s) 582
SfoI GGCGCC 1 cut(s) 156
Sfr274I CTCGAG 1 cut(s) 264
SinI GGWCC 1 cut(s) 235
SlaI CTCGAG 1 cut(s) 264
SmlI CTYRAG 3 cut(s) 92, 264, 305
SmoI CTYRAG 3 cut(s) 92, 264, 305
Sse9I AATT 1 cut(s) 691
SseBI AGGCCT 1 cut(s) 610
SsiI CCGC 3 cut(s) 135, 138, 181
SspDI GGCGCC 1 cut(s) 154
SspMI CTAG 3 cut(s) 224, 629, 672
SstI GAGCTC 1 cut(s) 582
StuI AGGCCT 1 cut(s) 610
StyD4I CCNGG 1 cut(s) 664
TaaI ACNGT 1 cut(s) 502
TaiI ACGT 1 cut(s) 386
TaqI TCGA 1 cut(s) 265
TasI AATT 1 cut(s) 691
TatI WGTACW 1 cut(s) 331
TauI GCSGC 1 cut(s) 140
TfiI GAWTC 2 cut(s) 23, 317
TseI GCWGC 1 cut(s) 572
TspDTI ATGAA 1 cut(s) 495
VpaK11BI GGWCC 1 cut(s) 235
XhoI CTCGAG 1 cut(s) 264
XspI CTAG 3 cut(s) 224, 629, 672
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.