Rorug03G0163000

S-adenosyl-L-methionine-dependent protein-lysine N- methyltransferase that methylates elongation factor 1-alpha

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0012069
Physical Location & Seq
Reverse (-)
13574367 .. 13578284
3918 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0163000.1

Sequence Viewer

Length: 660 bp
ATGGAGATTAGTAACATTGAAATGAAGCTCTCAAAGTTCAGGAGGATTTGTGTGTTCTGTGGGAGTAGCCAAGGCAAGAAGACCAGCTACCAAGACGCTGCCACTGAGCTTGGAAAAGAGCTGGTTTCAAGGAACATTGATCTGGTCTATGGAGGAGGCAGCATAGGCCTAATGGGTTTGGTTTCTCAAGCTGTGCATGATGGTGGTCGGCATGTCATTGGAGTTATTCCCAAGACGCTCATGCCTCGAGAGATCACTGGTGAAACTGTGGGGGAAGTGAAGGCAGTTGCAGACATGCATCAAAGAAAGGCAGAGATGGCTAAGCATTCAGATGCCTTTATTGCATTGCCTGGTGGTTATGGAACTCTGGAAGAGTTACTTGAAGTGATAACTTGGGCTCAGCTTGGAATTCATGACAAACCGGTGGGATTGCTGAATGTTGATGGATACTACAATTCCTTGCTGTCATTCATTGATACAGCTGTGGAAGAAGGATTTGTTAGCCCAAATGCACGCCACATTATTATATCAGCACCAACAGCAATGGAGTTGGTGAAGAAATTGGAGGATTATGTTCCATGTCATGAAAGAGTTGCTTCAAAGTTGAACTGGGAGATGGGACAGCTTGGCTACACTCAAGAATTCGGTATCTCTAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
N6-adenineMlase

Protein Analysis

219

Amino Acids

23.98

Weight (kDa)

5.81

Isoelectric Point (pI)

37.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LDcluster4 PF18306 21 - 146 1.2e-14 SLOG cluster4 family
Lysine_decarbox PF03641 58 - 188 6.3e-50 Possible lysine decarboxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012069)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G58470
fragaria_vesca FvH4_6g21270
malus_domestica MD14G1048200.v1.1
prunus_persica Prupe.7G087000_v2.0.a1
pyrus_communis pycom14g03970
rosa_chinensis RchiOBHm_Chr3g0477741 RchiOBHm_Chr3g0477781
rosa_laevigata RLG00000023692
rosa_multiflora Rmu_sc0017665.1_g000001 Rmu_sc0026290.1_g000001
rosa_roxburghii Rroxscaffold_6G00403710
rosa_rugosa Rorug03G0162900 Rorug03G0163000 Rorug03G0163100
rosa_samantha Rh3AG213400 Rh3BG247200 Rh3CG241000 Rh3DG240300
rosa_wichuraiana Rw3G019310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 2 cut(s) 408, 641
AgeI ACCGGT 1 cut(s) 421
AgsI TTSAA 5 cut(s) 20, 129, 383, 600, 607
AjnI CCWGG 1 cut(s) 349
AluBI AGCT 8 cut(s) 28, 87, 109, 121, 191, 403, 482, 625
AluI AGCT 8 cut(s) 28, 87, 109, 121, 191, 403, 482, 625
Ama87I CYCGRG 1 cut(s) 246
AoxI GGCC 1 cut(s) 166
ApeKI GCWGC 2 cut(s) 98, 159
ApoI RAATTY 2 cut(s) 408, 641
AsiGI ACCGGT 1 cut(s) 421
AsuHPI GGTGA 2 cut(s) 272, 565
AvaI CYCGRG 1 cut(s) 246
BaeI ACNNNNGTAYC 2 cut(s) 468, 501
BanII GRGCYC 1 cut(s) 400
BarI GAAGNNNNNNTAC 2 cut(s) 71, 103
BbsI GAAGAC 1 cut(s) 86
BbvI GCAGC 2 cut(s) 85, 171
BccI CCATC 4 cut(s) 194, 310, 437, 610
BciT130I CCWGG 1 cut(s) 351
BciVI GTATCC 1 cut(s) 440
BfaI CTAG 1 cut(s) 654
BfuI GTATCC 1 cut(s) 440
BisI GCNGC 2 cut(s) 99, 160
BlpI GCTNAGC 2 cut(s) 321, 399
BlsI GCNGC 2 cut(s) 100, 161
Bme1390I CCNGG 1 cut(s) 351
BmeT110I CYCGRG 1 cut(s) 246
BmrFI CCNGG 1 cut(s) 351
BmrI ACTGGG 1 cut(s) 619
BmsI GCATC 2 cut(s) 307, 322
BmuI ACTGGG 1 cut(s) 619
BpiI GAAGAC 1 cut(s) 86
Bpu1102I GCTNAGC 2 cut(s) 321, 399
BpuEI CTTGAG 2 cut(s) 171, 621
BsaJI CCNNGG 1 cut(s) 70
BsaWI WCCGGW 1 cut(s) 421
BsaXI ACNNNNNCTCC 2 cut(s) 34, 64
Bse118I RCCGGY 1 cut(s) 421
Bse1I ACTGG 2 cut(s) 262, 614
Bse3DI GCAATG 2 cut(s) 344, 549
BseBI CCWGG 1 cut(s) 351
BseDI CCNNGG 1 cut(s) 70
BseMI GCAATG 2 cut(s) 344, 549
BseMII CTCAG 2 cut(s) 96, 413
BseNI ACTGG 2 cut(s) 262, 614
BseRI GAGGAG 1 cut(s) 168
BseXI GCAGC 2 cut(s) 85, 171
BshFI GGCC 1 cut(s) 168
BshTI ACCGGT 1 cut(s) 421
BsiHKCI CYCGRG 1 cut(s) 246
BsiSI CCGG 1 cut(s) 422
BslFI GGGAC 1 cut(s) 633
BsmFI GGGAC 1 cut(s) 633
BsmI GAATGC 1 cut(s) 325
BsnI GGCC 1 cut(s) 168
BsoBI CYCGRG 1 cut(s) 246
Bsp1286I GDGCHC 1 cut(s) 400
Bsp143I GATC 2 cut(s) 139, 252
Bsp1720I GCTNAGC 2 cut(s) 321, 399
BspANI GGCC 1 cut(s) 168
BspCNI CTCAG 2 cut(s) 97, 412
BspHI TCATGA 2 cut(s) 412, 583
BsrDI GCAATG 2 cut(s) 344, 549
BsrFI RCCGGY 1 cut(s) 421
BsrI ACTGG 2 cut(s) 262, 614
BssAI RCCGGY 1 cut(s) 421
BssECI CCNNGG 1 cut(s) 70
BssMI GATC 2 cut(s) 139, 252
BssT1I CCWWGG 1 cut(s) 70
Bst2UI CCWGG 1 cut(s) 351
Bst4CI ACNGT 1 cut(s) 268
Bst6I CTCTTC 1 cut(s) 366
BstC8I GCNNGC 1 cut(s) 514
BstDEI CTNAG 3 cut(s) 105, 321, 399
BstKTI GATC 2 cut(s) 142, 255
BstMBI GATC 2 cut(s) 139, 252
BstMWI GCNNNNNNNGC 4 cut(s) 165, 317, 341, 539
BstNI CCWGG 1 cut(s) 351
BstNSI RCATGY 2 cut(s) 215, 298
BstSCI CCNGG 1 cut(s) 349
BstV1I GCAGC 2 cut(s) 85, 171
BstV2I GAAGAC 1 cut(s) 86
BsuI GTATCC 1 cut(s) 440
BsuRI GGCC 1 cut(s) 168
BtsIMutI CAGTG 2 cut(s) 102, 255
Cac8I GCNNGC 1 cut(s) 514
CciI TCATGA 2 cut(s) 412, 583
Cfr10I RCCGGY 1 cut(s) 421
CseI GACGC 2 cut(s) 104, 244
CspAI ACCGGT 1 cut(s) 421
CspCI CAANNNNNGTGG 2 cut(s) 91, 126
CviAII CATG 7 cut(s) 197, 212, 241, 295, 413, 579, 584
DdeI CTNAG 3 cut(s) 105, 321, 399
DpnI GATC 2 cut(s) 141, 254
DpnII GATC 2 cut(s) 139, 252
Eam1104I CTCTTC 1 cut(s) 366
EarI CTCTTC 1 cut(s) 366
Eco130I CCWWGG 1 cut(s) 70
Eco147I AGGCCT 1 cut(s) 168
Eco24I GRGCYC 1 cut(s) 400
Eco88I CYCGRG 1 cut(s) 246
EcoRI GAATTC 2 cut(s) 408, 641
EcoRII CCWGG 1 cut(s) 349
EcoT14I CCWWGG 1 cut(s) 70
EcoT22I ATGCAT 1 cut(s) 300
EcoT38I GRGCYC 1 cut(s) 400
ErhI CCWWGG 1 cut(s) 70
FaeI CATG 7 cut(s) 200, 215, 244, 298, 416, 582, 587
FalI AAGNNNNNCTT 4 cut(s) 363, 395, 580, 612
FaqI GGGAC 1 cut(s) 633
FatI CATG 7 cut(s) 196, 211, 240, 294, 412, 578, 583
Fnu4HI GCNGC 2 cut(s) 99, 160
FriOI GRGCYC 1 cut(s) 400
Fsp4HI GCNGC 2 cut(s) 99, 160
FspBI CTAG 1 cut(s) 654
GluI GCNGC 2 cut(s) 99, 160
HaeIII GGCC 1 cut(s) 168
HapII CCGG 1 cut(s) 422
HgaI GACGC 2 cut(s) 104, 244
Hin1II CATG 7 cut(s) 200, 215, 244, 298, 416, 582, 587
HpaII CCGG 1 cut(s) 422
HphI GGTGA 2 cut(s) 272, 565
Hpy188I TCNGA 1 cut(s) 331
Hpy188III TCNNGA 6 cut(s) 40, 248, 368, 413, 584, 638
HpyAV CCTTC 2 cut(s) 274, 485
HpyCH4III ACNGT 1 cut(s) 268
HpyCH4V TGCA 5 cut(s) 196, 290, 298, 344, 512
HpyF10VI GCNNNNNNNGC 4 cut(s) 165, 317, 341, 539
HpyF3I CTNAG 3 cut(s) 105, 321, 399
Hsp92II CATG 7 cut(s) 200, 215, 244, 298, 416, 582, 587
Kzo9I GATC 2 cut(s) 139, 252
Lsp1109I GCAGC 2 cut(s) 85, 171
LweI GCATC 2 cut(s) 307, 322
MaeI CTAG 1 cut(s) 654
MaeIII GTNAC 2 cut(s) 11, 375
MalI GATC 2 cut(s) 141, 254
MboI GATC 2 cut(s) 139, 252
MboII GAAGA 4 cut(s) 91, 383, 500, 568
MhlI GDGCHC 1 cut(s) 400
MluCI AATT 4 cut(s) 408, 454, 560, 641
MnlI CCTC 5 cut(s) 36, 146, 149, 255, 559
Mph1103I ATGCAT 1 cut(s) 300
MslI CAYNNNNRTG 3 cut(s) 20, 201, 330
MspA1I CMGCKG 1 cut(s) 482
MspI CCGG 1 cut(s) 422
MspR9I CCNGG 1 cut(s) 351
Mva1269I GAATGC 1 cut(s) 325
MvaI CCWGG 1 cut(s) 351
MwoI GCNNNNNNNGC 4 cut(s) 165, 317, 341, 539
NdeII GATC 2 cut(s) 139, 252
NlaIII CATG 7 cut(s) 200, 215, 244, 298, 416, 582, 587
NsiI ATGCAT 1 cut(s) 300
NspI RCATGY 2 cut(s) 215, 298
PaeR7I CTCGAG 1 cut(s) 246
PagI TCATGA 2 cut(s) 412, 583
PceI AGGCCT 1 cut(s) 168
PctI GAATGC 1 cut(s) 325
PinAI ACCGGT 1 cut(s) 421
PkrI GCNGC 2 cut(s) 100, 161
Psp6I CCWGG 1 cut(s) 349
PspGI CCWGG 1 cut(s) 349
PvuII CAGCTG 1 cut(s) 482
RseI CAYNNNNRTG 3 cut(s) 20, 201, 330
SatI GCNGC 2 cut(s) 99, 160
Sau3AI GATC 2 cut(s) 139, 252
ScrFI CCNGG 1 cut(s) 351
SduI GDGCHC 1 cut(s) 400
SetI ASST 9 cut(s) 30, 89, 111, 123, 193, 405, 484, 627, 659
SfaNI GCATC 2 cut(s) 307, 322
Sfr274I CTCGAG 1 cut(s) 246
SlaI CTCGAG 1 cut(s) 246
SmiMI CAYNNNNRTG 3 cut(s) 20, 201, 330
SmlI CTYRAG 3 cut(s) 186, 246, 636
SmoI CTYRAG 3 cut(s) 186, 246, 636
Sse9I AATT 4 cut(s) 408, 454, 560, 641
SseBI AGGCCT 1 cut(s) 168
SspMI CTAG 1 cut(s) 654
StuI AGGCCT 1 cut(s) 168
StyD4I CCNGG 1 cut(s) 349
StyI CCWWGG 1 cut(s) 70
TaaI ACNGT 1 cut(s) 268
TaqI TCGA 1 cut(s) 247
TasI AATT 4 cut(s) 408, 454, 560, 641
TscAI CASTG 2 cut(s) 109, 262
TseI GCWGC 2 cut(s) 98, 159
TspDTI ATGAA 4 cut(s) 38, 401, 460, 600
TspRI CASTG 2 cut(s) 109, 262
XapI RAATTY 2 cut(s) 408, 641
XceI RCATGY 2 cut(s) 215, 298
XhoI CTCGAG 1 cut(s) 246
XspI CTAG 1 cut(s) 654
Zsp2I ATGCAT 1 cut(s) 300
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.