Rorug03G0162900

S-adenosyl-L-methionine-dependent protein-lysine N- methyltransferase that methylates elongation factor 1-alpha

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0000142
Physical Location & Seq
Forward (+)
13568607 .. 13573296
4690 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0162900.1

Sequence Viewer

Length: 1926 bp
ATGTCTGCCCATTTGAGAAGGCCGCCGGAGGACATGGCTTGCATGAAGCTGGGAGTGAAGTCTGAAGCATTTCATCGGGAAGGCCAGACTTGGGTTTGCACAACTGGGCTACCGAGCGATATTATTGTTCAAGTTGGGGAAATGTCTTTCCACCTCCATAAGTTTCCATTGCTTTCTAAAAGTGGGCTATTAGAGCAGCTTATCGAACAGTGTTCTATGGAGGAAAGCTCACTTTGTGCATTGAAACTTCATGGTGTGCCTGGGGGAGCTAAAGCATTTGAGCTTGTAACCAAATTTTGCTATGGTGTCAAAATAGAGCTGACTGCGTTGAATGTTGTCATCCTTAGATGTGCAGCAGAATACCTCCGGATGACTGAAGAATATGAACAAGGAAACCTCATTGCACAAACAGAGGTTTTCCTTAATGAAGTCTTCGGCAGTTGGAGAGACTCCATAATAGCTCTTGAAACATGTGAAGAAGTGCAACCATATGTCGAAGAGCTCCACATTGTGTCAAGATGCATCGATTCACTGGCTACAAAAGCTTGTTCAGACCCAAAATTGTTCAGCAGGCCTATGTCGGGAGGAAGTGACATTAAGAAGCCAACAGGCACTGTGTCATGGAATGGAATTGCTGCTGAAAGTAAGCCACAATCTCCCGGCGAAAATTGGTGGTATGAAGATACATCCTTCCTGAGCTTACCTCTCTATAAACCCCTGATTCAAGCTGTCGAATCAAAAGGCATGAGGCCCGAGAGTATTGCAGCTTCTCTTATAACCTATGCTAGAAAGTATCTGCCCTTGATGAATAGGCTATCAAGCTTTAACGACGCAAATCATGCAAACTCGGGCACAAACATTTCTGATGAAGACCAAAGGGTTTTACTTGAAGAAATTGTGGGGTTGCTTCCTAATAAGAAAGGAGTAACATCCTCCAAGTTTCTGCTTAGGCTCTTAAGAACTGCTATGATATTACATGCAAGTCAATCATGTAGAGATAATTTGGAGAAAAGGGTGGGTGCACAGCTGGACCAAGCTGTTCTCATTGATCTTCTCATACCAAATATGGGCTACTCAGTGGAGACTCTTTATGACATAGACTGCATTCAGAGGATTCTTGATCATTTCATGTTGGTAAACCAGCCATATCCATCATCTTCTCCATGCATAATTGAAGAGGGGCAACTGATGACTGGCACTGATCCATTGACACCTATTACCATGGTGGCCAGTCTGGTTGATGGATATCTTGCCGAGGTAGCACCAGATGTTAATTTGAAGTTACCGAAATTTCAGGCACTCGCTGCTGTCATACCTGAATATGCCAGGCCGCTGGATGATGGCATTTATCATGCCATTGATGTTTTCTTGAAGGCACATCCTTGGCTTACAGATGCTGAAAGGGAGCTACTATGTAGACTCATGAACTGCAATAAACTCTCATTGGAAGCCAGCACCCATGCAGCGCAGAACGAAAGGTTACCTCTTCGAGTAATTGTTCAAGTCCTCTTCTTCGAACAGCTTAGACTTCGAACATCAATTTCTGGTTGGTTGTTTGTTGACAATCTTGAGAACTCACAAAATCCTAGTGGAAACCTTGGGCTTCCCAGCAAAAATGATGGGTCCAATCAAGTAGACCCTGCACAAGTTGAACGAGCTGTGGGTTTTGATCATGAGGTGAGGGAGCGTGTTTTGGAGCTCCAAAAGGAGTGTTCAAGTATGAAAAAGGAGCTTCAGAAGCTGGTAAAAAACAAGAGAAGTTGGAGCATTCTTCCCATTAGGTTAGGTTTTAGAAAAAAGGTTGATCCTTGTTGTCCAAAAGAATCAAAGGCCTCTGATAGAATGACACCAGCATCATGTTTCAGTGGGCAACAAAATCATGTGAATGGTGAAGTGCCTCATTCACCTGCAAAGCCTCAAACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
N6-adenineMlase

Protein Analysis

641

Amino Acids

71.5

Weight (kDa)

5.84

Isoelectric Point (pI)

44.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BTB PF00651 38 - 126 3.9e-07 BTB/POZ domain
NPH3 PF03000 223 - 485 1.2e-92 NPH3 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000142)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g13532 FvH4_2g28870 FvH4_3g30930 FvH4_3g37281 FvH4_3g37300 FvH4_3g37301 FvH4_3g37301 FvH4_4g23711 FvH4_5g02330 FvH4_6g16960 FvH4_6g16971 FvH4_6g21291 FvH4_6g21331 FvH4_6g22212 FvH4_6g22332 FvH4_6g36920 FvH4_6g37552 FvH4_6g37591 FvH4_6g39721 FvH4_6g46020 FvH4_6g47001 FvH4_6g47002 FvH4_6g47130 FvH4_6g47140 FvH4_6g47161 FvH4_6g47170 FvH4_6g47190 FvH4_6g49120 FvH4_6g49280 FvH4_6g49281 FvH4_6g49640 FvH4_6g49640
malus_domestica MD03G1085600.v1.1 MD09G1027500.v1.1 MD09G1050100.v1.1
prunus_persica Prupe.1G004300_v2.0.a1 Prupe.1G016300_v2.0.a1 Prupe.1G019300_v2.0.a1 Prupe.3G239100_v2.0.a1 Prupe.3G291200_v2.0.a1
pyrus_communis pycom08g02840 pycom08g02860 pycom08g02870
rosa_chinensis RchiOBHm_Chr2g0153831 RchiOBHm_Chr2g0166171 RchiOBHm_Chr2g0166201 RchiOBHm_Chr3g0476221 RchiOBHm_Chr4g0430971 RchiOBHm_Chr5g0067081 RchiOBHm_Chr7g0197821
rosa_laevigata RLG00000001284 RLG00000006996 RLG00000012710 RLG00000019299 RLG00000020285 RLG00000020444 RLG00000020466 RLG00000020591 RLG00000020665 RLG00000020730 RLG00000020732 RLG00000021449 RLG00000021592 RLG00000021593 RLG00000021594 RLG00000021596 RLG00000021597 RLG00000021801 RLG00000023534 RLG00000023538 RLG00000023693 RLG00000023694 RLG00000023699 RLG00000024196 RLG00000024214 RLG00000024220 RLG00000024244 RLG00000024608 RLG00000024612 RLG00000035157 RLG00000035906
rosa_multiflora Rmu_co8282815.1_g000001 Rmu_co8380495.1_g000001 Rmu_co8381111.1_g000001 Rmu_co8460513.1_g000001 Rmu_co8481947.1_g000001 Rmu_co8487937.1_g000001 Rmu_sc0000151.1_g000017 Rmu_sc0001427.1_g000003 Rmu_sc0001427.1_g000006 Rmu_sc0002611.1_g000008 Rmu_sc0003887.1_g000018 Rmu_sc0003887.1_g000019 Rmu_sc0003887.1_g000021 Rmu_sc0004119.1_g000004 Rmu_sc0004329.1_g000003 Rmu_sc0004485.1_g000024 Rmu_sc0005290.1_g000001 Rmu_sc0005609.1_g000006 Rmu_sc0006187.1_g000018 Rmu_sc0006218.1_g000032 Rmu_sc0006218.1_g000033 Rmu_sc0007150.1_g000003 Rmu_sc0007154.1_g000004 Rmu_sc0007154.1_g000005 Rmu_sc0007154.1_g000007 Rmu_sc0008314.1_g000012 Rmu_sc0010073.1_g000023 Rmu_sc0010073.1_g000025 Rmu_sc0011216.1_g000008 Rmu_sc0012445.1_g000005 Rmu_sc0013571.1_g000002 Rmu_sc0018648.1_g000002 Rmu_sc0020961.1_g000002 Rmu_sc0020961.1_g000003 Rmu_sc0023710.1_g000001 Rmu_sc0031310.1_g000008 Rmu_sc0033940.1_g000001 Rmu_sc0035493.1_g000001 Rmu_sc0039285.1_g000001
rosa_roxburghii Rroxscaffold_1G00014000 Rroxscaffold_1G00014020 Rroxscaffold_2G00082760 Rroxscaffold_2G00085090 Rroxscaffold_2G00085100 Rroxscaffold_2G00085140 Rroxscaffold_2G00086670 Rroxscaffold_2G00086680 Rroxscaffold_2G00098190 Rroxscaffold_2G00099270 Rroxscaffold_3G00227460 Rroxscaffold_3G00258190 Rroxscaffold_3G00259780 Rroxscaffold_6G00402940 Rroxscaffold_6G00410200 Rroxscaffold_6G00410210 Rroxscaffold_7G00184320
rosa_rugosa Rorug02G0416100 Rorug02G0416200 Rorug02G0520900 Rorug02G0521200 Rorug02G0521300 Rorug02G0534600 Rorug03G0116300 Rorug03G0162900 Rorug03G0162900 Rorug04G0241400 Rorug05G0380900 Rorug07G0043800
rosa_samantha Rh1AG115300 Rh2BG483700 Rh2BG509900 Rh2BG515700 Rh2BG515800 Rh2BG586500 Rh2BG586600 Rh2BG586700 Rh2BG598800 Rh2BG598900 Rh2BG599100 Rh2BG618400 Rh2BG630500 Rh2CG457400 Rh2CG484900 Rh3AG165300 Rh3AG212900 Rh3AG213300 Rh3BG145800 Rh3BG146100 Rh3BG235300 Rh3BG246500 Rh3BG247000 Rh3BG247100 Rh3CG240300 Rh3CG240800 Rh3CG240900 Rh3CG259600 Rh3DG147000 Rh3DG184800 Rh3DG184900 Rh3DG185000 Rh3DG239500 Rh3DG240100 Rh3DG240200 Rh3DG255800 Rh5BG458500 Rh5CG480400 Rh7BG170400 Rh7BG407900 Rh7CG176900
rosa_wichuraiana Rw2G038990 Rw2G041550 Rw2G047660 Rw2G048940 Rw4G026370 Rw6G008730 Rw7G014600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 776
AarI CACCTGC 1 cut(s) 1915
Acc36I ACCTGC 1 cut(s) 1915
AccI GTMKAC 2 cut(s) 1417, 1635
AccIII TCCGGA 1 cut(s) 366
AciI CCGC 2 cut(s) 23, 1331
AclWI GGATC 2 cut(s) 1196, 1799
AcoI YGGCCR 1 cut(s) 1227
AcsI RAATTY 2 cut(s) 293, 1289
AcuI CTGAAG 3 cut(s) 84, 396, 1718
AdeI CACNNNGTG 2 cut(s) 236, 511
AfiI CCNNNNNNNGG 3 cut(s) 91, 581, 1067
AflII CTTAAG 1 cut(s) 955
AflIII ACRYGT 1 cut(s) 470
AjnI CCWGG 2 cut(s) 259, 1325
Alw21I GWGCWC 3 cut(s) 504, 1024, 1701
Alw26I GTCTC 2 cut(s) 441, 1076
Alw44I GTGCAC 1 cut(s) 1020
AlwI GGATC 2 cut(s) 1196, 1799
AlwNI CAGNNNCTG 3 cut(s) 614, 1397, 1741
Ama87I CYCGRG 2 cut(s) 752, 847
Aor13HI TCCGGA 1 cut(s) 366
AoxI GGCC 7 cut(s) 20, 82, 572, 749, 1227, 1328, 1830
ApaLI GTGCAC 1 cut(s) 1020
ApeKI GCWGC 6 cut(s) 196, 353, 635, 764, 1304, 1463
ApoI RAATTY 2 cut(s) 293, 1289
Asp700I GAANNNNTTC 1 cut(s) 69
AspLEI GCGC 1 cut(s) 1468
AspS9I GGNCC 3 cut(s) 750, 1030, 1623
AsuC2I CCSGG 1 cut(s) 660
AsuHPI GGTGA 3 cut(s) 1690, 1896, 1901
AsuII TTCGAA 2 cut(s) 1515, 1531
AvaI CYCGRG 2 cut(s) 752, 847
AvaII GGWCC 2 cut(s) 1030, 1623
BaeGI GKGCMC 2 cut(s) 854, 1024
BalI TGGCCA 1 cut(s) 1229
BanII GRGCYC 2 cut(s) 504, 1701
BarI GAAGNNNNNNTAC 2 cut(s) 751, 783
BbsI GAAGAC 2 cut(s) 424, 876
Bbv12I GWGCWC 3 cut(s) 504, 1024, 1701
BbvI GCAGC 6 cut(s) 208, 365, 622, 776, 1291, 1475
BccI CCATC 4 cut(s) 1159, 1235, 1334, 1613
BcgI CGANNNNNNTGC 2 cut(s) 743, 777
BciT130I CCWGG 2 cut(s) 261, 1327
BclI TGATCA 2 cut(s) 1120, 1669
BcnI CCSGG 1 cut(s) 660
BcoDI GTCTC 2 cut(s) 441, 1076
BfaI CTAG 2 cut(s) 786, 1587
BfrI CTTAAG 1 cut(s) 955
BfuAI ACCTGC 1 cut(s) 1915
BisI GCNGC 8 cut(s) 23, 197, 354, 636, 765, 1305, 1331, 1464
BlsI GCNGC 8 cut(s) 24, 198, 355, 637, 766, 1306, 1332, 1465
Bme1390I CCNGG 3 cut(s) 261, 660, 1327
Bme18I GGWCC 2 cut(s) 1030, 1623
BmeT110I CYCGRG 2 cut(s) 752, 847
BmgT120I GGNCC 3 cut(s) 750, 1030, 1623
BmiI GGNNCC 1 cut(s) 1624
BmrFI CCNGG 3 cut(s) 261, 660, 1327
BmrI ACTGGG 1 cut(s) 114
BmsI GCATC 4 cut(s) 509, 531, 1384, 1862
BmuI ACTGGG 1 cut(s) 114
BpiI GAAGAC 2 cut(s) 424, 876
BplI GAGNNNNNCTC 4 cut(s) 212, 244, 688, 720
Bpu10I CCTNAGC 2 cut(s) 695, 947
Bpu14I TTCGAA 2 cut(s) 1515, 1531
BpuEI CTTGAG 1 cut(s) 1589
BpuMI CCSGG 1 cut(s) 660
Bsa29I ATCGAT 1 cut(s) 525
BsaBI GATNNNNATC 1 cut(s) 1245
BsaJI CCNNGG 5 cut(s) 260, 1221, 1254, 1382, 1597
BsaWI WCCGGW 1 cut(s) 366
Bsc4I CCNNNNNNNGG 3 cut(s) 91, 581, 1067
Bse1I ACTGG 4 cut(s) 109, 537, 1198, 1230
Bse3DI GCAATG 2 cut(s) 167, 399
Bse8I GATNNNNATC 1 cut(s) 1245
BseAI TCCGGA 1 cut(s) 366
BseBI CCWGG 2 cut(s) 261, 1327
BseCI ATCGAT 1 cut(s) 525
BseDI CCNNGG 5 cut(s) 260, 1221, 1254, 1382, 1597
BseGI GGATG 6 cut(s) 339, 375, 686, 929, 1342, 1378
BseJI GATNNNNATC 1 cut(s) 1245
BseLI CCNNNNNNNGG 3 cut(s) 91, 581, 1067
BseMI GCAATG 2 cut(s) 167, 399
BseMII CTCAG 2 cut(s) 686, 1089
BseNI ACTGG 4 cut(s) 109, 537, 1198, 1230
BseSI GKGCMC 2 cut(s) 854, 1024
BseXI GCAGC 6 cut(s) 208, 365, 622, 776, 1291, 1475
BseYI CCCAGC 2 cut(s) 49, 1607
BsgI GTGCAG 2 cut(s) 372, 1626
BshFI GGCC 7 cut(s) 22, 84, 574, 751, 1229, 1330, 1832
BshVI ATCGAT 1 cut(s) 525
BsiHKAI GWGCWC 3 cut(s) 504, 1024, 1701
BsiHKCI CYCGRG 2 cut(s) 752, 847
BsiSI CCGG 3 cut(s) 26, 367, 660
BslI CCNNNNNNNGG 3 cut(s) 91, 581, 1067
BsmAI GTCTC 2 cut(s) 441, 1076
BsmI GAATGC 2 cut(s) 1104, 1767
BsnI GGCC 7 cut(s) 22, 84, 574, 751, 1229, 1330, 1832
BsoBI CYCGRG 2 cut(s) 752, 847
Bsp119I TTCGAA 2 cut(s) 1515, 1531
Bsp1286I GDGCHC 4 cut(s) 504, 854, 1024, 1701
Bsp13I TCCGGA 1 cut(s) 366
Bsp143I GATC 5 cut(s) 1048, 1120, 1201, 1669, 1804
Bsp19I CCATGG 1 cut(s) 1221
BspACI CCGC 2 cut(s) 23, 1331
BspANI GGCC 7 cut(s) 22, 84, 574, 751, 1229, 1330, 1832
BspCNI CTCAG 2 cut(s) 687, 1088
BspDI ATCGAT 1 cut(s) 525
BspEI TCCGGA 1 cut(s) 366
BspHI TCATGA 2 cut(s) 1422, 1672
BspLI GGNNCC 1 cut(s) 1624
BspMI ACCTGC 1 cut(s) 1915
BspPI GGATC 2 cut(s) 1196, 1799
BspQI GCTCTTC 1 cut(s) 492
BspT104I TTCGAA 2 cut(s) 1515, 1531
BspTI CTTAAG 1 cut(s) 955
BsrDI GCAATG 2 cut(s) 167, 399
BsrI ACTGG 4 cut(s) 109, 537, 1198, 1230
BssECI CCNNGG 5 cut(s) 260, 1221, 1254, 1382, 1597
BssMI GATC 5 cut(s) 1048, 1120, 1201, 1669, 1804
BssT1I CCWWGG 3 cut(s) 1221, 1382, 1597
Bst2UI CCWGG 2 cut(s) 261, 1327
Bst4CI ACNGT 2 cut(s) 210, 616
Bst6I CTCTTC 4 cut(s) 492, 1170, 1491, 1514
BstAFI CTTAAG 1 cut(s) 955
BstAPI GCANNNNNTGC 2 cut(s) 839, 1304
BstBI TTCGAA 2 cut(s) 1515, 1531
BstC8I GCNNGC 3 cut(s) 40, 572, 1453
BstDEI CTNAG 5 cut(s) 344, 695, 947, 1075, 1523
BstDSI CCRYGG 1 cut(s) 1221
BstEII GGTNACC 1 cut(s) 1479
BstF5I GGATG 6 cut(s) 339, 375, 686, 929, 1342, 1378
BstHHI GCGC 1 cut(s) 1468
BstKTI GATC 5 cut(s) 1051, 1123, 1204, 1672, 1807
BstMAI GTCTC 2 cut(s) 441, 1076
BstMBI GATC 5 cut(s) 1048, 1120, 1201, 1669, 1804
BstMWI GCNNNNNNNGC 6 cut(s) 193, 542, 839, 1259, 1304, 1738
BstNI CCWGG 2 cut(s) 261, 1327
BstNSI RCATGY 2 cut(s) 474, 980
BstPI GGTNACC 1 cut(s) 1479
BstSCI CCNGG 3 cut(s) 259, 658, 1325
BstSLI GKGCMC 2 cut(s) 854, 1024
BstV1I GCAGC 6 cut(s) 208, 365, 622, 776, 1291, 1475
BstV2I GAAGAC 2 cut(s) 424, 876
BstXI CCANNNNNNTGG 1 cut(s) 1333
Bsu15I ATCGAT 1 cut(s) 525
BsuRI GGCC 7 cut(s) 22, 84, 574, 751, 1229, 1330, 1832
BsuTUI ATCGAT 1 cut(s) 525
BtgI CCRYGG 1 cut(s) 1221
BtsCI GGATG 6 cut(s) 339, 375, 686, 929, 1342, 1378
BtsIMutI CAGTG 6 cut(s) 215, 530, 612, 1083, 1197, 1870
BveI ACCTGC 1 cut(s) 1915
Cac8I GCNNGC 3 cut(s) 40, 572, 1453
CaiI CAGNNNCTG 3 cut(s) 614, 1397, 1741
CciI TCATGA 2 cut(s) 1422, 1672
CfoI GCGC 1 cut(s) 1468
Cfr13I GGNCC 3 cut(s) 750, 1030, 1623
ClaI ATCGAT 1 cut(s) 525
CseI GACGC 1 cut(s) 839
DdeI CTNAG 5 cut(s) 344, 695, 947, 1075, 1523
DpnI GATC 5 cut(s) 1050, 1122, 1203, 1671, 1806
DpnII GATC 5 cut(s) 1048, 1120, 1201, 1669, 1804
DraIII CACNNNGTG 2 cut(s) 236, 511
EaeI YGGCCR 1 cut(s) 1227
Eam1104I CTCTTC 4 cut(s) 492, 1170, 1491, 1514
EarI CTCTTC 4 cut(s) 492, 1170, 1491, 1514
Ecl136II GAGCTC 2 cut(s) 502, 1699
Eco130I CCWWGG 3 cut(s) 1221, 1382, 1597
Eco147I AGGCCT 2 cut(s) 574, 1832
Eco24I GRGCYC 2 cut(s) 504, 1701
Eco32I GATATC 1 cut(s) 1247
Eco47I GGWCC 2 cut(s) 1030, 1623
Eco53kI GAGCTC 2 cut(s) 502, 1699
Eco57I CTGAAG 3 cut(s) 84, 396, 1718
Eco88I CYCGRG 2 cut(s) 752, 847
Eco91I GGTNACC 1 cut(s) 1479
EcoICRI GAGCTC 2 cut(s) 502, 1699
EcoO65I GGTNACC 1 cut(s) 1479
EcoRII CCWGG 2 cut(s) 259, 1325
EcoRV GATATC 1 cut(s) 1247
EcoT14I CCWWGG 3 cut(s) 1221, 1382, 1597
EcoT22I ATGCAT 2 cut(s) 524, 1169
EcoT38I GRGCYC 2 cut(s) 504, 1701
ErhI CCWWGG 3 cut(s) 1221, 1382, 1597
FauNDI CATATG 1 cut(s) 490
FbaI TGATCA 2 cut(s) 1120, 1669
FblI GTMKAC 2 cut(s) 1417, 1635
Fnu4HI GCNGC 8 cut(s) 23, 197, 354, 636, 765, 1305, 1331, 1464
FokI GGATG 6 cut(s) 326, 382, 673, 916, 1349, 1365
FriOI GRGCYC 2 cut(s) 504, 1701
Fsp4HI GCNGC 8 cut(s) 23, 197, 354, 636, 765, 1305, 1331, 1464
FspBI CTAG 2 cut(s) 786, 1587
GlaI GCGC 1 cut(s) 1467
GluI GCNGC 8 cut(s) 23, 197, 354, 636, 765, 1305, 1331, 1464
GsaI CCCAGC 2 cut(s) 53, 1611
HaeIII GGCC 7 cut(s) 22, 84, 574, 751, 1229, 1330, 1832
HapII CCGG 3 cut(s) 26, 367, 660
HgaI GACGC 1 cut(s) 839
HhaI GCGC 1 cut(s) 1468
Hin6I GCGC 1 cut(s) 1466
HinP1I GCGC 1 cut(s) 1466
HincII GTYRAC 1 cut(s) 1561
HindII GTYRAC 1 cut(s) 1561
HindIII AAGCTT 2 cut(s) 543, 820
HinfI GANTC 8 cut(s) 449, 527, 721, 734, 1084, 1114, 1419, 1823
HpaII CCGG 3 cut(s) 26, 367, 660
HphI GGTGA 3 cut(s) 1690, 1896, 1901
Hpy166II GTNNAC 5 cut(s) 1022, 1138, 1418, 1561, 1636
Hpy188I TCNGA 6 cut(s) 64, 553, 865, 1110, 1737, 1837
Hpy8I GTNNAC 5 cut(s) 1022, 1138, 1418, 1561, 1636
Hpy99I CGWCG 1 cut(s) 833
HpyAV CCTTC 4 cut(s) 12, 74, 700, 1366
HpyCH4III ACNGT 2 cut(s) 210, 616
HpyF10VI GCNNNNNNNGC 6 cut(s) 193, 542, 839, 1259, 1304, 1738
HpyF3I CTNAG 5 cut(s) 344, 695, 947, 1075, 1523
HspAI GCGC 1 cut(s) 1466
Kpn2I TCCGGA 1 cut(s) 366
Ksp22I TGATCA 2 cut(s) 1120, 1669
Kzo9I GATC 5 cut(s) 1048, 1120, 1201, 1669, 1804
LguI GCTCTTC 1 cut(s) 492
LmnI GCTCC 8 cut(s) 266, 507, 1405, 1684, 1696, 1704, 1729, 1764
Lsp1109I GCAGC 6 cut(s) 208, 365, 622, 776, 1291, 1475
LweI GCATC 4 cut(s) 509, 531, 1384, 1862
MaeI CTAG 2 cut(s) 786, 1587
MaeIII GTNAC 5 cut(s) 286, 590, 925, 1281, 1479
MalI GATC 5 cut(s) 1050, 1122, 1203, 1671, 1806
MboI GATC 5 cut(s) 1048, 1120, 1201, 1669, 1804
MhlI GDGCHC 4 cut(s) 504, 854, 1024, 1701
MlsI TGGCCA 1 cut(s) 1229
MluNI TGGCCA 1 cut(s) 1229
MlyI GAGTC 3 cut(s) 443, 1078, 1413
MmeI TCCRAC 2 cut(s) 422, 1742
Mox20I TGGCCA 1 cut(s) 1229
Mph1103I ATGCAT 2 cut(s) 524, 1169
MroI TCCGGA 1 cut(s) 366
MroXI GAANNNNTTC 1 cut(s) 69
MscI TGGCCA 1 cut(s) 1229
MseI TTAA 6 cut(s) 423, 597, 825, 956, 1272, 1924
MslI CAYNNNNRTG 1 cut(s) 1884
Msp20I TGGCCA 1 cut(s) 1229
MspA1I CMGCKG 2 cut(s) 1027, 1333
MspCI CTTAAG 1 cut(s) 955
MspI CCGG 3 cut(s) 26, 367, 660
MspR9I CCNGG 3 cut(s) 261, 660, 1327
Mva1269I GAATGC 2 cut(s) 1104, 1767
MvaI CCWGG 2 cut(s) 261, 1327
MwoI GCNNNNNNNGC 6 cut(s) 193, 542, 839, 1259, 1304, 1738
NciI CCSGG 1 cut(s) 660
NcoI CCATGG 1 cut(s) 1221
NdeI CATATG 1 cut(s) 490
NdeII GATC 5 cut(s) 1048, 1120, 1201, 1669, 1804
NlaIV GGNNCC 1 cut(s) 1624
NmeAIII GCCGAG 1 cut(s) 1279
NmuCI GTSAC 1 cut(s) 590
NsiI ATGCAT 2 cut(s) 524, 1169
NspI RCATGY 2 cut(s) 474, 980
NspV TTCGAA 2 cut(s) 1515, 1531
PagI TCATGA 2 cut(s) 1422, 1672
PaqCI CACCTGC 1 cut(s) 1915
PceI AGGCCT 2 cut(s) 574, 1832
PciI ACATGT 1 cut(s) 470
PciSI GCTCTTC 1 cut(s) 492
PctI GAATGC 2 cut(s) 1104, 1767
PdmI GAANNNNTTC 1 cut(s) 69
PfeI GAWTC 5 cut(s) 527, 721, 734, 1114, 1823
PkrI GCNGC 8 cut(s) 24, 198, 355, 637, 766, 1306, 1332, 1465
PleI GAGTC 3 cut(s) 443, 1078, 1413
PpsI GAGTC 3 cut(s) 443, 1078, 1413
PscI ACATGT 1 cut(s) 470
PsiI TTATAA 1 cut(s) 776
Psp124BI GAGCTC 2 cut(s) 504, 1701
Psp6I CCWGG 2 cut(s) 259, 1325
PspEI GGTNACC 1 cut(s) 1479
PspFI CCCAGC 2 cut(s) 49, 1607
PspGI CCWGG 2 cut(s) 259, 1325
PspN4I GGNNCC 1 cut(s) 1624
PspPI GGNCC 3 cut(s) 750, 1030, 1623
PstNI CAGNNNCTG 3 cut(s) 614, 1397, 1741
PvuII CAGCTG 1 cut(s) 1027
RseI CAYNNNNRTG 1 cut(s) 1884
SacI GAGCTC 2 cut(s) 504, 1701
SapI GCTCTTC 1 cut(s) 492
SaqAI TTAA 6 cut(s) 423, 597, 825, 956, 1272, 1924
SatI GCNGC 8 cut(s) 23, 197, 354, 636, 765, 1305, 1331, 1464
Sau3AI GATC 5 cut(s) 1048, 1120, 1201, 1669, 1804
Sau96I GGNCC 3 cut(s) 750, 1030, 1623
SchI GAGTC 3 cut(s) 443, 1078, 1413
ScrFI CCNGG 3 cut(s) 261, 660, 1327
SduI GDGCHC 4 cut(s) 504, 854, 1024, 1701
SfaNI GCATC 4 cut(s) 509, 531, 1384, 1862
SfuI TTCGAA 2 cut(s) 1515, 1531
SinI GGWCC 2 cut(s) 1030, 1623
SmiMI CAYNNNNRTG 1 cut(s) 1884
SmlI CTYRAG 2 cut(s) 955, 1568
SmoI CTYRAG 2 cut(s) 955, 1568
SseBI AGGCCT 2 cut(s) 574, 1832
SsiI CCGC 2 cut(s) 23, 1331
SspMI CTAG 2 cut(s) 786, 1587
SstI GAGCTC 2 cut(s) 504, 1701
StuI AGGCCT 2 cut(s) 574, 1832
StyD4I CCNGG 3 cut(s) 259, 658, 1325
StyI CCWWGG 3 cut(s) 1221, 1382, 1597
TaaI ACNGT 2 cut(s) 210, 616
TaqI TCGA 7 cut(s) 204, 495, 525, 732, 1489, 1515, 1531
TauI GCSGC 2 cut(s) 25, 1333
TfiI GAWTC 5 cut(s) 527, 721, 734, 1114, 1823
Tru1I TTAA 6 cut(s) 423, 597, 825, 956, 1272, 1924
Tru9I TTAA 6 cut(s) 423, 597, 825, 956, 1272, 1924
TscAI CASTG 6 cut(s) 215, 537, 619, 1083, 1204, 1870
TseFI GTSAC 1 cut(s) 590
TseI GCWGC 6 cut(s) 196, 353, 635, 764, 1304, 1463
Tsp45I GTSAC 1 cut(s) 590
TspRI CASTG 6 cut(s) 215, 537, 619, 1083, 1204, 1870
Vha464I CTTAAG 1 cut(s) 955
VneI GTGCAC 1 cut(s) 1020
VpaK11BI GGWCC 2 cut(s) 1030, 1623
XapI RAATTY 2 cut(s) 293, 1289
XceI RCATGY 2 cut(s) 474, 980
XmiI GTMKAC 2 cut(s) 1417, 1635
XmnI GAANNNNTTC 1 cut(s) 69
XspI CTAG 2 cut(s) 786, 1587
Zsp2I ATGCAT 2 cut(s) 524, 1169
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.