Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb6

Orthogroup ID

OG0000142

Physical Location & Seq

Genomic Coordinates Forward (+)

36872547 .. 36873812

Gene Structure 1266 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_6g49120.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1266 bp

ATGAAGATGTTGACCAGACGGAGTTTTTCAAGAAAGTTGATATGGTGTCGTGATTTGATCCGCTGCAAGTTCTTCACTAGAAAACGATACGAGTTAGAAGACGAAGGAGAAGGAGGAGGTTGGTTCGAGCAACTCCCAGATGACATCATGCAACTGATTCTGCAGCGACTGCCCCTGTCGGATTATCTGAATTGTCGTGAGGTAAGCCGTAGTTGGCGAGCCGCAATTGACAGCAAGCTTTGGCTTCCTGCTGCTCATCCTCAACTCCCAAGGCTTGTGACCAGTTCTAGACTAGGAAGCGACATGCTTGCATCCTACATCAAAAAAGATGATGATAACTACTTGCGTATTGTAAGCCATCCGAAAATTTGGAAAATAAAAACCGATCCTCCCGCAAATTGTTATTGTGCTGGATCAGTTGAGGGATGGCTGATCATAACCACTAGTAGATCATATGTTGAGAGGTTCTTCTTCTGGAATCCAGTATCAGGTGCTCGAGTTAAGCTCCCAACACATCATCCCAGCCGCGGCAGTTTCCCGGAAGTAAAAGTAGTAGCCTCTTCAGTACCAACGACAACAGAGTGTTATGTGAGTTGCCTTCACTTCAGACAAGAAGGTTGTTTGGCCTTTTGCACGCCCACTGATAGATCATGGACCTTGATTAAGGCAGAGAAAGCTCTTGAATTTTGGGAGATAGAAATACTTGATGGGAAATTATATGCGCTAGGTAGAGAAGCATTAGAGTATGTGATGGTGTTTGACATCCATTTAGACACACATGCCTACACATCACAAAGGTTGGTTACTCTTCACCCCAACATTAACAGCATAGTTGATGGCGGAACCTACTACCGGACATGTTACTTGGCAAAATGTTGTGAATCAAAGGACCTGTTTATGGTTTTGTGGCATGATGGTCTATGTCGTCCGAACTACCAAAGATCATTGCAAGTGTTAAAGTTAGCGGACAATAATAAGTGGGTAGAGATTAATGACCTTGGTGATCGGGCATTGTTTATGGCCACGCTCAACAACAAATTTATCACTTGTCATGATAAAACACTTGAAAGAAACTGTATCTATTTTGTTTTACGGAAAAGATATGATTATGCACACAACTTTGGGGTGTTTTCCTTGACAGATAGGAGCTTCAAAACTATGACTTCTCTAGCATCAAAAAAAAAAACTGTGACTTCTCTCCGGCATTATCCTCGTCTTTTGTGGGCGATACAACCTAGTGCTTGGTTCACACCAAATCCTTACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000463 GO:0000470 GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0006139 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0010467 GO:0010468 GO:0016070 GO:0016072 GO:0019219 GO:0019222 GO:0022613 GO:0030684 GO:0030686 GO:0031323 GO:0031974 GO:0031981 GO:0032991 GO:0034470 GO:0034641 GO:0034660 GO:0042254 GO:0042273 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044087 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044452 GO:0044464 GO:0046483 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0070013 GO:0070545 GO:0071704 GO:0071840 GO:0080090 GO:0090069 GO:0090304 GO:0097159 GO:1901360 GO:1901363 GO:1990904 GO:2000232

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

BRCT_2 Pescadillo_N

Protein Analysis

422

Amino Acids

49.12

Weight (kDa)

9.08

Isoelectric Point (pI)

46.12

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
F-box-like	PF12937	42 - 81	8.2e-10	F-box-like
F-box	PF00646	43 - 79	4.4e-11	F-box domain
Beta-prop_KIB1-4	PF03478	130 - 378	2e-37	KIB1-4 beta-propeller

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000142)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g13532 FvH4_2g28870 FvH4_3g30930 FvH4_3g37281 FvH4_3g37300 FvH4_3g37301 FvH4_3g37301 FvH4_4g23711 FvH4_5g02330 FvH4_6g16960 FvH4_6g16971 FvH4_6g21291 FvH4_6g21331 FvH4_6g22212 FvH4_6g22332 FvH4_6g36920 FvH4_6g37552 FvH4_6g37591 FvH4_6g39721 FvH4_6g46020 FvH4_6g47001 FvH4_6g47002 FvH4_6g47130 FvH4_6g47140 FvH4_6g47161 FvH4_6g47170 FvH4_6g47190 FvH4_6g49120 FvH4_6g49280 FvH4_6g49281 FvH4_6g49640 FvH4_6g49640
malus_domestica	MD03G1085600.v1.1 MD09G1027500.v1.1 MD09G1050100.v1.1
prunus_persica	Prupe.1G004300_v2.0.a1 Prupe.1G016300_v2.0.a1 Prupe.1G019300_v2.0.a1 Prupe.3G239100_v2.0.a1 Prupe.3G291200_v2.0.a1
pyrus_communis	pycom08g02840 pycom08g02860 pycom08g02870
rosa_chinensis	RchiOBHm_Chr2g0153831 RchiOBHm_Chr2g0166171 RchiOBHm_Chr2g0166201 RchiOBHm_Chr3g0476221 RchiOBHm_Chr4g0430971 RchiOBHm_Chr5g0067081 RchiOBHm_Chr7g0197821
rosa_laevigata	RLG00000001284 RLG00000006996 RLG00000012710 RLG00000019299 RLG00000020285 RLG00000020444 RLG00000020466 RLG00000020591 RLG00000020665 RLG00000020730 RLG00000020732 RLG00000021449 RLG00000021592 RLG00000021593 RLG00000021594 RLG00000021596 RLG00000021597 RLG00000021801 RLG00000023534 RLG00000023538 RLG00000023693 RLG00000023694 RLG00000023699 RLG00000024196 RLG00000024214 RLG00000024220 RLG00000024244 RLG00000024608 RLG00000024612 RLG00000035157 RLG00000035906
rosa_multiflora	Rmu_co8282815.1_g000001 Rmu_co8380495.1_g000001 Rmu_co8381111.1_g000001 Rmu_co8460513.1_g000001 Rmu_co8481947.1_g000001 Rmu_co8487937.1_g000001 Rmu_sc0000151.1_g000017 Rmu_sc0001427.1_g000003 Rmu_sc0001427.1_g000006 Rmu_sc0002611.1_g000008 Rmu_sc0003887.1_g000018 Rmu_sc0003887.1_g000019 Rmu_sc0003887.1_g000021 Rmu_sc0004119.1_g000004 Rmu_sc0004329.1_g000003 Rmu_sc0004485.1_g000024 Rmu_sc0005290.1_g000001 Rmu_sc0005609.1_g000006 Rmu_sc0006187.1_g000018 Rmu_sc0006218.1_g000032 Rmu_sc0006218.1_g000033 Rmu_sc0007150.1_g000003 Rmu_sc0007154.1_g000004 Rmu_sc0007154.1_g000005 Rmu_sc0007154.1_g000007 Rmu_sc0008314.1_g000012 Rmu_sc0010073.1_g000023 Rmu_sc0010073.1_g000025 Rmu_sc0011216.1_g000008 Rmu_sc0012445.1_g000005 Rmu_sc0013571.1_g000002 Rmu_sc0018648.1_g000002 Rmu_sc0020961.1_g000002 Rmu_sc0020961.1_g000003 Rmu_sc0023710.1_g000001 Rmu_sc0031310.1_g000008 Rmu_sc0033940.1_g000001 Rmu_sc0035493.1_g000001 Rmu_sc0039285.1_g000001
rosa_roxburghii	Rroxscaffold_1G00014000 Rroxscaffold_1G00014020 Rroxscaffold_2G00082760 Rroxscaffold_2G00085090 Rroxscaffold_2G00085100 Rroxscaffold_2G00085140 Rroxscaffold_2G00086670 Rroxscaffold_2G00086680 Rroxscaffold_2G00098190 Rroxscaffold_2G00099270 Rroxscaffold_3G00227460 Rroxscaffold_3G00258190 Rroxscaffold_3G00259780 Rroxscaffold_6G00402940 Rroxscaffold_6G00410200 Rroxscaffold_6G00410210 Rroxscaffold_7G00184320
rosa_rugosa	Rorug02G0416100 Rorug02G0416200 Rorug02G0520900 Rorug02G0521200 Rorug02G0521300 Rorug02G0534600 Rorug03G0116300 Rorug03G0162900 Rorug03G0162900 Rorug04G0241400 Rorug05G0380900 Rorug07G0043800
rosa_samantha	Rh1AG115300 Rh2BG483700 Rh2BG509900 Rh2BG515700 Rh2BG515800 Rh2BG586500 Rh2BG586600 Rh2BG586700 Rh2BG598800 Rh2BG598900 Rh2BG599100 Rh2BG618400 Rh2BG630500 Rh2CG457400 Rh2CG484900 Rh3AG165300 Rh3AG212900 Rh3AG213300 Rh3BG145800 Rh3BG146100 Rh3BG235300 Rh3BG246500 Rh3BG247000 Rh3BG247100 Rh3CG240300 Rh3CG240800 Rh3CG240900 Rh3CG259600 Rh3DG147000 Rh3DG184800 Rh3DG184900 Rh3DG185000 Rh3DG239500 Rh3DG240100 Rh3DG240200 Rh3DG255800 Rh5BG458500 Rh5CG480400 Rh7BG170400 Rh7BG407900 Rh7CG176900
rosa_wichuraiana	Rw2G038990 Rw2G041550 Rw2G047660 Rw2G048940 Rw4G026370 Rw6G008730 Rw7G014600

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccII	CGCG	1 cut(s)	528
AciI	CCGC	7 cut(s)	61, 222, 393, 526, 528, 840, 965
AclWI	GGATC	3 cut(s)	52, 380, 421
AcoI	YGGCCR	1 cut(s)	1020
AcsI	RAATTY	3 cut(s)	366, 683, 1037
AcuI	CTGAAG	2 cut(s)	546, 589
AfaI	GTAC	1 cut(s)	567
AfiI	CCNNNNNNNGG	4 cut(s)	488, 527, 852, 898
AflIII	ACRYGT	1 cut(s)	857
AgsI	TTSAA	4 cut(s)	30, 683, 1067, 1153
AhlI	ACTAGT	1 cut(s)	443
AluBI	AGCT	4 cut(s)	238, 505, 677, 1149
AluI	AGCT	4 cut(s)	238, 505, 677, 1149
Alw21I	GWGCWC	1 cut(s)	496
AlwI	GGATC	3 cut(s)	52, 380, 421
AlwNI	CAGNNNCTG	1 cut(s)	169
Ama87I	CYCGRG	1 cut(s)	495
AoxI	GGCC	2 cut(s)	624, 1020
ApeKI	GCWGC	3 cut(s)	63, 163, 251
ApoI	RAATTY	3 cut(s)	366, 683, 1037
AseI	ATTAAT	1 cut(s)	990
AspLEI	GCGC	1 cut(s)	724
AspS9I	GGNCC	2 cut(s)	654, 889
AsuC2I	CCSGG	1 cut(s)	539
AsuHPI	GGTGA	2 cut(s)	803, 1013
AvaI	CYCGRG	1 cut(s)	495
AvaII	GGWCC	2 cut(s)	654, 889
BalI	TGGCCA	1 cut(s)	1022
BbsI	GAAGAC	1 cut(s)	105
Bbv12I	GWGCWC	1 cut(s)	496
BbvI	GCAGC	3 cut(s)	50, 175, 238
BccI	CCATC	6 cut(s)	366, 420, 701, 745, 830, 908
BceAI	ACGGC	1 cut(s)	192
BcgI	CGANNNNNNTGC	4 cut(s)	290, 324, 1193, 1227
BclI	TGATCA	1 cut(s)	432
BcnI	CCSGG	1 cut(s)	539
BcuI	ACTAGT	1 cut(s)	443
BfaI	CTAG	8 cut(s)	78, 288, 293, 444, 725, 1169, 1236, 1264
BfmI	CTRYAG	1 cut(s)	161
BisI	GCNGC	6 cut(s)	64, 164, 222, 252, 526, 529
BlsI	GCNGC	6 cut(s)	65, 165, 223, 253, 527, 530
Bme1390I	CCNGG	1 cut(s)	539
Bme18I	GGWCC	2 cut(s)	654, 889
BmeT110I	CYCGRG	1 cut(s)	495
BmgT120I	GGNCC	2 cut(s)	654, 889
BmiI	GGNNCC	1 cut(s)	844
BmrFI	CCNGG	1 cut(s)	539
BmsI	GCATC	2 cut(s)	320, 1181
BpiI	GAAGAC	1 cut(s)	105
BplI	GAGNNNNNCTC	2 cut(s)	489, 521
BpuMI	CCSGG	1 cut(s)	539
BsaJI	CCNNGG	3 cut(s)	269, 526, 997
BsaWI	WCCGGW	1 cut(s)	852
BsaXI	ACNNNNNCTCC	2 cut(s)	373, 403
Bsc4I	CCNNNNNNNGG	4 cut(s)	488, 527, 852, 898
Bse1I	ACTGG	2 cut(s)	282, 482
Bse3DI	GCAATG	1 cut(s)	944
BseDI	CCNNGG	3 cut(s)	269, 526, 997
BseGI	GGATG	6 cut(s)	256, 311, 358, 431, 517, 762
BseLI	CCNNNNNNNGG	4 cut(s)	488, 527, 852, 898
BseMI	GCAATG	1 cut(s)	944
BseNI	ACTGG	2 cut(s)	282, 482
BseRI	GAGGAG	1 cut(s)	129
BseXI	GCAGC	3 cut(s)	50, 175, 238
BseYI	CCCAGC	1 cut(s)	521
Bsh1236I	CGCG	1 cut(s)	528
BshFI	GGCC	2 cut(s)	626, 1022
BsiHKAI	GWGCWC	1 cut(s)	496
BsiHKCI	CYCGRG	1 cut(s)	495
BsiSI	CCGG	3 cut(s)	539, 853, 1201
BslI	CCNNNNNNNGG	4 cut(s)	488, 527, 852, 898
BsnI	GGCC	2 cut(s)	626, 1022
BsoBI	CYCGRG	1 cut(s)	495
Bsp1286I	GDGCHC	1 cut(s)	496
Bsp143I	GATC	8 cut(s)	57, 385, 413, 432, 449, 647, 941, 1003
BspACI	CCGC	7 cut(s)	61, 222, 393, 526, 528, 840, 965
BspANI	GGCC	2 cut(s)	626, 1022
BspFNI	CGCG	1 cut(s)	528
BspHI	TCATGA	1 cut(s)	1051
BspLI	GGNNCC	1 cut(s)	844
BspMAI	CTGCAG	1 cut(s)	165
BspPI	GGATC	3 cut(s)	52, 380, 421
BsrDI	GCAATG	1 cut(s)	944
BsrI	ACTGG	2 cut(s)	282, 482
BssECI	CCNNGG	3 cut(s)	269, 526, 997
BssMI	GATC	8 cut(s)	57, 385, 413, 432, 449, 647, 941, 1003
BssT1I	CCWWGG	2 cut(s)	269, 997
Bst4CI	ACNGT	2 cut(s)	1076, 1189
Bst6I	CTCTTC	2 cut(s)	565, 813
BstAPI	GCANNNNNTGC	1 cut(s)	169
BstC8I	GCNNGC	4 cut(s)	219, 236, 309, 635
BstDSI	CCRYGG	1 cut(s)	526
BstF5I	GGATG	6 cut(s)	256, 311, 358, 431, 517, 762
BstFNI	CGCG	1 cut(s)	528
BstHHI	GCGC	1 cut(s)	724
BstKTI	GATC	8 cut(s)	60, 388, 416, 435, 452, 650, 944, 1006
BstMBI	GATC	8 cut(s)	57, 385, 413, 432, 449, 647, 941, 1003
BstMWI	GCNNNNNNNGC	2 cut(s)	169, 674
BstNSI	RCATGY	3 cut(s)	307, 782, 861
BstSCI	CCNGG	1 cut(s)	537
BstSFI	CTRYAG	1 cut(s)	161
BstUI	CGCG	1 cut(s)	528
BstV1I	GCAGC	3 cut(s)	50, 175, 238
BstV2I	GAAGAC	1 cut(s)	105
BsuRI	GGCC	2 cut(s)	626, 1022
BtgI	CCRYGG	1 cut(s)	526
BtsCI	GGATG	6 cut(s)	256, 311, 358, 431, 517, 762
BtsIMutI	CAGTG	1 cut(s)	639
Cac8I	GCNNGC	4 cut(s)	219, 236, 309, 635
CaiI	CAGNNNCTG	1 cut(s)	169
CciI	TCATGA	1 cut(s)	1051
CfoI	GCGC	1 cut(s)	724
Cfr13I	GGNCC	2 cut(s)	654, 889
Cfr42I	CCGCGG	1 cut(s)	529
Csp6I	GTAC	1 cut(s)	566
CviAII	CATG	7 cut(s)	148, 304, 651, 779, 858, 911, 1052
CviQI	GTAC	1 cut(s)	566
DpnI	GATC	8 cut(s)	59, 387, 415, 434, 451, 649, 943, 1005
DpnII	GATC	8 cut(s)	57, 385, 413, 432, 449, 647, 941, 1003
EaeI	YGGCCR	1 cut(s)	1020
Eam1104I	CTCTTC	2 cut(s)	565, 813
EarI	CTCTTC	2 cut(s)	565, 813
EciI	GGCGGA	1 cut(s)	855
Eco130I	CCWWGG	2 cut(s)	269, 997
Eco47I	GGWCC	2 cut(s)	654, 889
Eco57I	CTGAAG	2 cut(s)	546, 589
Eco88I	CYCGRG	1 cut(s)	495
EcoO109I	RGGNCCY	1 cut(s)	889
EcoT14I	CCWWGG	2 cut(s)	269, 997
ErhI	CCWWGG	2 cut(s)	269, 997
FaeI	CATG	7 cut(s)	151, 307, 654, 782, 861, 914, 1055
FatI	CATG	7 cut(s)	147, 303, 650, 778, 857, 910, 1051
FauI	CCCGC	1 cut(s)	400
FauNDI	CATATG	1 cut(s)	454
FbaI	TGATCA	1 cut(s)	432
Fnu4HI	GCNGC	6 cut(s)	64, 164, 222, 252, 526, 529
FokI	GGATG	6 cut(s)	243, 298, 345, 438, 504, 749
Fsp4HI	GCNGC	6 cut(s)	64, 164, 222, 252, 526, 529
FspBI	CTAG	8 cut(s)	78, 288, 293, 444, 725, 1169, 1236, 1264
GlaI	GCGC	1 cut(s)	723
GluI	GCNGC	6 cut(s)	64, 164, 222, 252, 526, 529
GsaI	CCCAGC	1 cut(s)	525
HaeIII	GGCC	2 cut(s)	626, 1022
HapII	CCGG	3 cut(s)	539, 853, 1201
HhaI	GCGC	1 cut(s)	724
Hin1II	CATG	7 cut(s)	151, 307, 654, 782, 861, 914, 1055
Hin6I	GCGC	1 cut(s)	722
HinP1I	GCGC	1 cut(s)	722
HincII	GTYRAC	1 cut(s)	12
HindII	GTYRAC	1 cut(s)	12
HindIII	AAGCTT	1 cut(s)	236
HinfI	GANTC	3 cut(s)	157, 478, 881
HpaII	CCGG	3 cut(s)	539, 853, 1201
HphI	GGTGA	2 cut(s)	803, 1013
Hpy166II	GTNNAC	2 cut(s)	12, 1248
Hpy188I	TCNGA	5 cut(s)	181, 189, 363, 608, 930
Hpy188III	TCNNGA	7 cut(s)	30, 50, 197, 288, 475, 680, 1052
Hpy8I	GTNNAC	2 cut(s)	12, 1248
HpyAV	CCTTC	4 cut(s)	98, 104, 608, 608
HpyCH4III	ACNGT	2 cut(s)	1076, 1189
HpyCH4V	TGCA	7 cut(s)	66, 151, 163, 311, 633, 949, 1112
HpyF10VI	GCNNNNNNNGC	2 cut(s)	169, 674
Hsp92II	CATG	7 cut(s)	151, 307, 654, 782, 861, 914, 1055
HspAI	GCGC	1 cut(s)	722
Ksp22I	TGATCA	1 cut(s)	432
KspI	CCGCGG	1 cut(s)	529
Kzo9I	GATC	8 cut(s)	57, 385, 413, 432, 449, 647, 941, 1003
LmnI	GCTCC	2 cut(s)	510, 1146
Lsp1109I	GCAGC	3 cut(s)	50, 175, 238
LweI	GCATC	2 cut(s)	320, 1181
MaeI	CTAG	8 cut(s)	78, 288, 293, 444, 725, 1169, 1236, 1264
MaeIII	GTNAC	4 cut(s)	277, 802, 860, 1189
MalI	GATC	8 cut(s)	59, 387, 415, 434, 451, 649, 943, 1005
MboI	GATC	8 cut(s)	57, 385, 413, 432, 449, 647, 941, 1003
MboII	GAAGA	7 cut(s)	16, 64, 110, 460, 463, 552, 800
MfeI	CAATTG	1 cut(s)	225
MhlI	GDGCHC	1 cut(s)	496
MlsI	TGGCCA	1 cut(s)	1022
MluCI	AATT	7 cut(s)	190, 225, 366, 397, 683, 713, 1037
MluNI	TGGCCA	1 cut(s)	1022
MmeI	TCCRAC	1 cut(s)	159
MnlI	CCTC	9 cut(s)	107, 110, 193, 270, 399, 415, 456, 568, 1221
Mox20I	TGGCCA	1 cut(s)	1022
MscI	TGGCCA	1 cut(s)	1022
MseI	TTAA	5 cut(s)	501, 663, 822, 956, 990
Msp20I	TGGCCA	1 cut(s)	1022
MspA1I	CMGCKG	2 cut(s)	63, 528
MspI	CCGG	3 cut(s)	539, 853, 1201
MspR9I	CCNGG	1 cut(s)	539
MunI	CAATTG	1 cut(s)	225
MvnI	CGCG	1 cut(s)	528
MwoI	GCNNNNNNNGC	2 cut(s)	169, 674
NciI	CCSGG	1 cut(s)	539
NdeI	CATATG	1 cut(s)	454
NdeII	GATC	8 cut(s)	57, 385, 413, 432, 449, 647, 941, 1003
NlaIII	CATG	7 cut(s)	151, 307, 654, 782, 861, 914, 1055
NlaIV	GGNNCC	1 cut(s)	844
NmuCI	GTSAC	2 cut(s)	277, 1189
NspI	RCATGY	3 cut(s)	307, 782, 861
PaeR7I	CTCGAG	1 cut(s)	495
PagI	TCATGA	1 cut(s)	1051
PciI	ACATGT	1 cut(s)	857
PfeI	GAWTC	3 cut(s)	157, 478, 881
PfoI	TCCNGGA	1 cut(s)	537
PkrI	GCNGC	6 cut(s)	65, 165, 223, 253, 527, 530
PpuMI	RGGWCCY	1 cut(s)	889
PscI	ACATGT	1 cut(s)	857
PshBI	ATTAAT	1 cut(s)	990
Psp5II	RGGWCCY	1 cut(s)	889
PspFI	CCCAGC	1 cut(s)	521
PspN4I	GGNNCC	1 cut(s)	844
PspPI	GGNCC	2 cut(s)	654, 889
PspPPI	RGGWCCY	1 cut(s)	889
PspXI	VCTCGAGB	1 cut(s)	495
PstI	CTGCAG	1 cut(s)	165
PstNI	CAGNNNCTG	1 cut(s)	169
RsaI	GTAC	1 cut(s)	567
RsaNI	GTAC	1 cut(s)	566
SacII	CCGCGG	1 cut(s)	529
SaqAI	TTAA	5 cut(s)	501, 663, 822, 956, 990
SatI	GCNGC	6 cut(s)	64, 164, 222, 252, 526, 529
Sau3AI	GATC	8 cut(s)	57, 385, 413, 432, 449, 647, 941, 1003
Sau96I	GGNCC	2 cut(s)	654, 889
ScrFI	CCNGG	1 cut(s)	539
SduI	GDGCHC	1 cut(s)	496
SfaNI	GCATC	2 cut(s)	320, 1181
SfcI	CTRYAG	1 cut(s)	161
Sfr274I	CTCGAG	1 cut(s)	495
Sfr303I	CCGCGG	1 cut(s)	529
SgrBI	CCGCGG	1 cut(s)	529
SinI	GGWCC	2 cut(s)	654, 889
SlaI	CTCGAG	1 cut(s)	495
SmlI	CTYRAG	1 cut(s)	495
SmoI	CTYRAG	1 cut(s)	495
SpeI	ACTAGT	1 cut(s)	443
Sse9I	AATT	7 cut(s)	190, 225, 366, 397, 683, 713, 1037
SsiI	CCGC	7 cut(s)	61, 222, 393, 526, 528, 840, 965
SspMI	CTAG	8 cut(s)	78, 288, 293, 444, 725, 1169, 1236, 1264
StyD4I	CCNGG	1 cut(s)	537
StyI	CCWWGG	2 cut(s)	269, 997
TaaI	ACNGT	2 cut(s)	1076, 1189
TaqI	TCGA	2 cut(s)	126, 496
TasI	AATT	7 cut(s)	190, 225, 366, 397, 683, 713, 1037
TauI	GCSGC	3 cut(s)	224, 528, 531
TfiI	GAWTC	3 cut(s)	157, 478, 881
Tru1I	TTAA	5 cut(s)	501, 663, 822, 956, 990
Tru9I	TTAA	5 cut(s)	501, 663, 822, 956, 990
TscAI	CASTG	1 cut(s)	646
TseFI	GTSAC	2 cut(s)	277, 1189
TseI	GCWGC	3 cut(s)	63, 163, 251
Tsp45I	GTSAC	2 cut(s)	277, 1189
TspDTI	ATGAA	1 cut(s)	17
TspGWI	ACGGA	2 cut(s)	34, 1108
TspRI	CASTG	1 cut(s)	646
VpaK11BI	GGWCC	2 cut(s)	654, 889
VspI	ATTAAT	1 cut(s)	990
XapI	RAATTY	3 cut(s)	366, 683, 1037
XbaI	TCTAGA	1 cut(s)	287
XceI	RCATGY	3 cut(s)	307, 782, 861
XhoI	CTCGAG	1 cut(s)	495
XspI	CTAG	8 cut(s)	78, 288, 293, 444, 725, 1169, 1236, 1264

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.