Required for maturation of ribosomal RNAs and formation of the large ribosomal subunit
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 228, 960 |
| AarI | CACCTGC | 1 cut(s) | 771 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 1010 |
| AatII | GACGTC | 1 cut(s) | 368 |
| Acc36I | ACCTGC | 1 cut(s) | 771 |
| AccI | GTMKAC | 1 cut(s) | 258 |
| AccII | CGCG | 4 cut(s) | 485, 555, 841, 843 |
| AciI | CCGC | 6 cut(s) | 483, 485, 555, 839, 843, 1023 |
| AclI | AACGTT | 1 cut(s) | 637 |
| AclWI | GGATC | 3 cut(s) | 491, 746, 1143 |
| AcsI | RAATTY | 3 cut(s) | 376, 656, 1100 |
| AcuI | CTGAAG | 2 cut(s) | 477, 1020 |
| AcyI | GRCGYC | 2 cut(s) | 16, 365 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 431, 539 |
| AgsI | TTSAA | 8 cut(s) | 105, 161, 190, 405, 421, 569, 1054, 1126 |
| AhlI | ACTAGT | 1 cut(s) | 715 |
| AjnI | CCWGG | 2 cut(s) | 598, 1144 |
| AluBI | AGCT | 3 cut(s) | 448, 457, 480 |
| AluI | AGCT | 3 cut(s) | 448, 457, 480 |
| Alw21I | GWGCWC | 2 cut(s) | 482, 906 |
| Alw26I | GTCTC | 1 cut(s) | 977 |
| AlwI | GGATC | 3 cut(s) | 491, 746, 1143 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 218 |
| Ama87I | CYCGRG | 1 cut(s) | 325 |
| AoxI | GGCC | 3 cut(s) | 486, 596, 911 |
| ApeKI | GCWGC | 1 cut(s) | 116 |
| ApoI | RAATTY | 3 cut(s) | 376, 656, 1100 |
| AseI | ATTAAT | 1 cut(s) | 336 |
| AspLEI | GCGC | 1 cut(s) | 843 |
| AspS9I | GGNCC | 3 cut(s) | 42, 870, 912 |
| AsuC2I | CCSGG | 2 cut(s) | 574, 915 |
| AsuHPI | GGTGA | 3 cut(s) | 377, 893, 1099 |
| AsuII | TTCGAA | 1 cut(s) | 274 |
| AvaI | CYCGRG | 1 cut(s) | 325 |
| AvaII | GGWCC | 2 cut(s) | 42, 870 |
| BanII | GRGCYC | 2 cut(s) | 332, 482 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 159, 191 |
| BbsI | GAAGAC | 5 cut(s) | 19, 255, 404, 1008, 1061 |
| Bbv12I | GWGCWC | 2 cut(s) | 482, 906 |
| BbvI | GCAGC | 1 cut(s) | 103 |
| BccI | CCATC | 4 cut(s) | 662, 832, 865, 934 |
| BceAI | ACGGC | 1 cut(s) | 945 |
| BciT130I | CCWGG | 2 cut(s) | 600, 1146 |
| BciVI | GTATCC | 1 cut(s) | 1030 |
| BcnI | CCSGG | 2 cut(s) | 574, 915 |
| BcoDI | GTCTC | 1 cut(s) | 977 |
| BcuI | ACTAGT | 1 cut(s) | 715 |
| BfaI | CTAG | 4 cut(s) | 95, 539, 716, 1016 |
| BfmI | CTRYAG | 1 cut(s) | 235 |
| BfuAI | ACCTGC | 1 cut(s) | 771 |
| BfuI | GTATCC | 1 cut(s) | 1030 |
| BglII | AGATCT | 1 cut(s) | 535 |
| BisI | GCNGC | 4 cut(s) | 117, 486, 839, 844 |
| BlsI | GCNGC | 4 cut(s) | 118, 487, 840, 845 |
| Bme1390I | CCNGG | 4 cut(s) | 574, 600, 915, 1146 |
| Bme18I | GGWCC | 2 cut(s) | 42, 870 |
| BmeT110I | CYCGRG | 1 cut(s) | 325 |
| BmgT120I | GGNCC | 3 cut(s) | 42, 870, 912 |
| BmrFI | CCNGG | 4 cut(s) | 574, 600, 915, 1146 |
| BmsI | GCATC | 2 cut(s) | 58, 286 |
| BpiI | GAAGAC | 5 cut(s) | 19, 255, 404, 1008, 1061 |
| BpmI | CTGGAG | 1 cut(s) | 516 |
| Bpu14I | TTCGAA | 1 cut(s) | 274 |
| BpuEI | CTTGAG | 2 cut(s) | 89, 251 |
| BpuMI | CCSGG | 2 cut(s) | 574, 915 |
| Bsa29I | ATCGAT | 1 cut(s) | 194 |
| BsaAI | YACGTR | 2 cut(s) | 305, 682 |
| BsaBI | GATNNNNATC | 1 cut(s) | 702 |
| BsaHI | GRCGYC | 2 cut(s) | 16, 365 |
| BsaJI | CCNNGG | 4 cut(s) | 179, 473, 483, 1145 |
| BsaWI | WCCGGW | 1 cut(s) | 461 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 431, 539 |
| Bse1I | ACTGG | 1 cut(s) | 897 |
| Bse8I | GATNNNNATC | 1 cut(s) | 702 |
| BseBI | CCWGG | 2 cut(s) | 600, 1146 |
| BseCI | ATCGAT | 1 cut(s) | 194 |
| BseDI | CCNNGG | 4 cut(s) | 179, 473, 483, 1145 |
| BseGI | GGATG | 6 cut(s) | 31, 301, 468, 582, 673, 949 |
| BseJI | GATNNNNATC | 1 cut(s) | 702 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 431, 539 |
| BseMII | CTCAG | 1 cut(s) | 225 |
| BseNI | ACTGG | 1 cut(s) | 897 |
| BseXI | GCAGC | 1 cut(s) | 103 |
| BseYI | CCCAGC | 1 cut(s) | 453 |
| Bsh1236I | CGCG | 4 cut(s) | 485, 555, 841, 843 |
| BshFI | GGCC | 3 cut(s) | 488, 598, 913 |
| BshVI | ATCGAT | 1 cut(s) | 194 |
| BsiHKAI | GWGCWC | 2 cut(s) | 482, 906 |
| BsiHKCI | CYCGRG | 1 cut(s) | 325 |
| BsiSI | CCGG | 3 cut(s) | 462, 573, 915 |
| BslFI | GGGAC | 1 cut(s) | 865 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 431, 539 |
| BsmAI | GTCTC | 1 cut(s) | 977 |
| BsmBI | CGTCTC | 1 cut(s) | 977 |
| BsmFI | GGGAC | 1 cut(s) | 865 |
| BsnI | GGCC | 3 cut(s) | 488, 598, 913 |
| BsoBI | CYCGRG | 1 cut(s) | 325 |
| Bsp119I | TTCGAA | 1 cut(s) | 274 |
| Bsp1286I | GDGCHC | 3 cut(s) | 332, 482, 906 |
| Bsp143I | GATC | 5 cut(s) | 496, 535, 550, 751, 1135 |
| Bsp19I | CCATGG | 2 cut(s) | 179, 473 |
| BspACI | CCGC | 6 cut(s) | 483, 485, 555, 839, 843, 1023 |
| BspANI | GGCC | 3 cut(s) | 488, 598, 913 |
| BspCNI | CTCAG | 1 cut(s) | 224 |
| BspDI | ATCGAT | 1 cut(s) | 194 |
| BspFNI | CGCG | 4 cut(s) | 485, 555, 841, 843 |
| BspHI | TCATGA | 2 cut(s) | 607, 661 |
| BspMI | ACCTGC | 1 cut(s) | 771 |
| BspPI | GGATC | 3 cut(s) | 491, 746, 1143 |
| BspT104I | TTCGAA | 1 cut(s) | 274 |
| BsrI | ACTGG | 1 cut(s) | 897 |
| BssECI | CCNNGG | 4 cut(s) | 179, 473, 483, 1145 |
| BssMI | GATC | 5 cut(s) | 496, 535, 550, 751, 1135 |
| BssNI | GRCGYC | 2 cut(s) | 16, 365 |
| BssT1I | CCWWGG | 2 cut(s) | 179, 473 |
| Bst2UI | CCWGG | 2 cut(s) | 600, 1146 |
| Bst4CI | ACNGT | 3 cut(s) | 239, 256, 693 |
| Bst6I | CTCTTC | 2 cut(s) | 271, 496 |
| BstACI | GRCGYC | 2 cut(s) | 16, 365 |
| BstBAI | YACGTR | 2 cut(s) | 305, 682 |
| BstBI | TTCGAA | 1 cut(s) | 274 |
| BstC8I | GCNNGC | 1 cut(s) | 596 |
| BstDEI | CTNAG | 2 cut(s) | 211, 393 |
| BstDSI | CCRYGG | 3 cut(s) | 179, 473, 483 |
| BstF5I | GGATG | 6 cut(s) | 31, 301, 468, 582, 673, 949 |
| BstFNI | CGCG | 4 cut(s) | 485, 555, 841, 843 |
| BstHHI | GCGC | 1 cut(s) | 843 |
| BstKTI | GATC | 5 cut(s) | 499, 538, 553, 754, 1138 |
| BstMAI | GTCTC | 1 cut(s) | 977 |
| BstMBI | GATC | 5 cut(s) | 496, 535, 550, 751, 1135 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 454, 910 |
| BstNI | CCWGG | 2 cut(s) | 600, 1146 |
| BstSCI | CCNGG | 4 cut(s) | 572, 598, 913, 1144 |
| BstSFI | CTRYAG | 1 cut(s) | 235 |
| BstSNI | TACGTA | 1 cut(s) | 682 |
| BstUI | CGCG | 4 cut(s) | 485, 555, 841, 843 |
| BstV1I | GCAGC | 1 cut(s) | 103 |
| BstV2I | GAAGAC | 5 cut(s) | 19, 255, 404, 1008, 1061 |
| BstX2I | RGATCY | 4 cut(s) | 496, 535, 751, 1135 |
| BstYI | RGATCY | 4 cut(s) | 496, 535, 751, 1135 |
| Bsu15I | ATCGAT | 1 cut(s) | 194 |
| BsuI | GTATCC | 1 cut(s) | 1030 |
| BsuRI | GGCC | 3 cut(s) | 488, 598, 913 |
| BsuTUI | ATCGAT | 1 cut(s) | 194 |
| BtgI | CCRYGG | 3 cut(s) | 179, 473, 483 |
| BtsCI | GGATG | 6 cut(s) | 31, 301, 468, 582, 673, 949 |
| BveI | ACCTGC | 1 cut(s) | 771 |
| Cac8I | GCNNGC | 1 cut(s) | 596 |
| CaiI | CAGNNNCTG | 1 cut(s) | 218 |
| CciI | TCATGA | 2 cut(s) | 607, 661 |
| CfoI | GCGC | 1 cut(s) | 843 |
| Cfr13I | GGNCC | 3 cut(s) | 42, 870, 912 |
| Cfr42I | CCGCGG | 1 cut(s) | 486 |
| ClaI | ATCGAT | 1 cut(s) | 194 |
| CseI | GACGC | 2 cut(s) | 24, 74 |
| CviAII | CATG | 6 cut(s) | 180, 474, 608, 615, 662, 1115 |
| DdeI | CTNAG | 2 cut(s) | 211, 393 |
| DpnI | GATC | 5 cut(s) | 498, 537, 552, 753, 1137 |
| DpnII | GATC | 5 cut(s) | 496, 535, 550, 751, 1135 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 1010 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 1010 |
| Eam1104I | CTCTTC | 2 cut(s) | 271, 496 |
| EarI | CTCTTC | 2 cut(s) | 271, 496 |
| EciI | GGCGGA | 1 cut(s) | 1012 |
| Ecl136II | GAGCTC | 1 cut(s) | 480 |
| Eco105I | TACGTA | 1 cut(s) | 682 |
| Eco130I | CCWWGG | 2 cut(s) | 179, 473 |
| Eco24I | GRGCYC | 2 cut(s) | 332, 482 |
| Eco47I | GGWCC | 2 cut(s) | 42, 870 |
| Eco53kI | GAGCTC | 1 cut(s) | 480 |
| Eco57I | CTGAAG | 2 cut(s) | 477, 1020 |
| Eco88I | CYCGRG | 1 cut(s) | 325 |
| EcoICRI | GAGCTC | 1 cut(s) | 480 |
| EcoRII | CCWGG | 2 cut(s) | 598, 1144 |
| EcoT14I | CCWWGG | 2 cut(s) | 179, 473 |
| EcoT38I | GRGCYC | 2 cut(s) | 332, 482 |
| ErhI | CCWWGG | 2 cut(s) | 179, 473 |
| Esp3I | CGTCTC | 1 cut(s) | 977 |
| FaeI | CATG | 6 cut(s) | 183, 477, 611, 618, 665, 1118 |
| FaqI | GGGAC | 1 cut(s) | 865 |
| FatI | CATG | 6 cut(s) | 179, 473, 607, 614, 661, 1114 |
| FblI | GTMKAC | 1 cut(s) | 258 |
| Fnu4HI | GCNGC | 4 cut(s) | 117, 486, 839, 844 |
| FokI | GGATG | 6 cut(s) | 38, 308, 455, 589, 680, 956 |
| FriOI | GRGCYC | 2 cut(s) | 332, 482 |
| Fsp4HI | GCNGC | 4 cut(s) | 117, 486, 839, 844 |
| FspBI | CTAG | 4 cut(s) | 95, 539, 716, 1016 |
| GlaI | GCGC | 1 cut(s) | 842 |
| GluI | GCNGC | 4 cut(s) | 117, 486, 839, 844 |
| GsaI | CCCAGC | 1 cut(s) | 457 |
| GsuI | CTGGAG | 1 cut(s) | 516 |
| HaeIII | GGCC | 3 cut(s) | 488, 598, 913 |
| HapII | CCGG | 3 cut(s) | 462, 573, 915 |
| HgaI | GACGC | 2 cut(s) | 24, 74 |
| HhaI | GCGC | 1 cut(s) | 843 |
| Hin1I | GRCGYC | 2 cut(s) | 16, 365 |
| Hin1II | CATG | 6 cut(s) | 183, 477, 611, 618, 665, 1118 |
| Hin6I | GCGC | 1 cut(s) | 841 |
| HinP1I | GCGC | 1 cut(s) | 841 |
| HindIII | AAGCTT | 1 cut(s) | 446 |
| HinfI | GANTC | 9 cut(s) | 101, 111, 215, 382, 421, 465, 611, 833, 1118 |
| HpaII | CCGG | 3 cut(s) | 462, 573, 915 |
| HphI | GGTGA | 3 cut(s) | 377, 893, 1099 |
| Hpy166II | GTNNAC | 3 cut(s) | 259, 976, 1158 |
| Hpy188I | TCNGA | 7 cut(s) | 110, 214, 358, 496, 511, 550, 697 |
| Hpy188III | TCNNGA | 7 cut(s) | 9, 56, 187, 418, 533, 608, 662 |
| Hpy8I | GTNNAC | 3 cut(s) | 259, 976, 1158 |
| Hpy99I | CGWCG | 1 cut(s) | 370 |
| HpyAV | CCTTC | 2 cut(s) | 138, 620 |
| HpyCH4III | ACNGT | 3 cut(s) | 239, 256, 693 |
| HpyCH4IV | ACGT | 6 cut(s) | 304, 365, 544, 637, 651, 681 |
| HpyCH4V | TGCA | 3 cut(s) | 516, 524, 594 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 454, 910 |
| HpyF3I | CTNAG | 2 cut(s) | 211, 393 |
| HpySE526I | ACGT | 6 cut(s) | 304, 365, 544, 637, 651, 681 |
| Hsp92I | GRCGYC | 2 cut(s) | 16, 365 |
| Hsp92II | CATG | 6 cut(s) | 183, 477, 611, 618, 665, 1118 |
| HspAI | GCGC | 1 cut(s) | 841 |
| KspI | CCGCGG | 1 cut(s) | 486 |
| Kzo9I | GATC | 5 cut(s) | 496, 535, 550, 751, 1135 |
| LmnI | GCTCC | 3 cut(s) | 477, 485, 901 |
| Lsp1109I | GCAGC | 1 cut(s) | 103 |
| LweI | GCATC | 2 cut(s) | 58, 286 |
| MaeI | CTAG | 4 cut(s) | 95, 539, 716, 1016 |
| MaeII | ACGT | 6 cut(s) | 304, 365, 544, 637, 651, 681 |
| MaeIII | GTNAC | 2 cut(s) | 147, 166 |
| MalI | GATC | 5 cut(s) | 498, 537, 552, 753, 1137 |
| MboI | GATC | 5 cut(s) | 496, 535, 550, 751, 1135 |
| MflI | RGATCY | 4 cut(s) | 496, 535, 751, 1135 |
| MhlI | GDGCHC | 3 cut(s) | 332, 482, 906 |
| MluCI | AATT | 8 cut(s) | 62, 223, 376, 409, 645, 656, 923, 1100 |
| MmeI | TCCRAC | 1 cut(s) | 273 |
| MnlI | CCTC | 8 cut(s) | 275, 499, 658, 883, 1036, 1048, 1072, 1126 |
| MseI | TTAA | 7 cut(s) | 198, 336, 444, 654, 758, 926, 935 |
| MslI | CAYNNNNRTG | 2 cut(s) | 666, 1040 |
| MspA1I | CMGCKG | 2 cut(s) | 457, 485 |
| MspI | CCGG | 3 cut(s) | 462, 573, 915 |
| MspR9I | CCNGG | 4 cut(s) | 574, 600, 915, 1146 |
| MvaI | CCWGG | 2 cut(s) | 600, 1146 |
| MvnI | CGCG | 4 cut(s) | 485, 555, 841, 843 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 454, 910 |
| NciI | CCSGG | 2 cut(s) | 574, 915 |
| NcoI | CCATGG | 2 cut(s) | 179, 473 |
| NdeII | GATC | 5 cut(s) | 496, 535, 550, 751, 1135 |
| NlaIII | CATG | 6 cut(s) | 183, 477, 611, 618, 665, 1118 |
| NmuCI | GTSAC | 1 cut(s) | 147 |
| NspV | TTCGAA | 1 cut(s) | 274 |
| PagI | TCATGA | 2 cut(s) | 607, 661 |
| PaqCI | CACCTGC | 1 cut(s) | 771 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 169 |
| PfeI | GAWTC | 9 cut(s) | 101, 111, 215, 382, 421, 465, 611, 833, 1118 |
| PflFI | GACNNNGTC | 1 cut(s) | 254 |
| PkrI | GCNGC | 4 cut(s) | 118, 487, 840, 845 |
| Ppu21I | YACGTR | 2 cut(s) | 305, 682 |
| PshBI | ATTAAT | 1 cut(s) | 336 |
| PsiI | TTATAA | 2 cut(s) | 228, 960 |
| Psp124BI | GAGCTC | 1 cut(s) | 482 |
| Psp1406I | AACGTT | 1 cut(s) | 637 |
| Psp6I | CCWGG | 2 cut(s) | 598, 1144 |
| PspFI | CCCAGC | 1 cut(s) | 453 |
| PspGI | CCWGG | 2 cut(s) | 598, 1144 |
| PspPI | GGNCC | 3 cut(s) | 42, 870, 912 |
| PstNI | CAGNNNCTG | 1 cut(s) | 218 |
| PsuI | RGATCY | 4 cut(s) | 496, 535, 751, 1135 |
| PsyI | GACNNNGTC | 1 cut(s) | 254 |
| PvuII | CAGCTG | 1 cut(s) | 457 |
| RseI | CAYNNNNRTG | 2 cut(s) | 666, 1040 |
| SacI | GAGCTC | 1 cut(s) | 482 |
| SacII | CCGCGG | 1 cut(s) | 486 |
| SaqAI | TTAA | 7 cut(s) | 198, 336, 444, 654, 758, 926, 935 |
| SatI | GCNGC | 4 cut(s) | 117, 486, 839, 844 |
| Sau3AI | GATC | 5 cut(s) | 496, 535, 550, 751, 1135 |
| Sau96I | GGNCC | 3 cut(s) | 42, 870, 912 |
| ScrFI | CCNGG | 4 cut(s) | 574, 600, 915, 1146 |
| SduI | GDGCHC | 3 cut(s) | 332, 482, 906 |
| SfaNI | GCATC | 2 cut(s) | 58, 286 |
| SfcI | CTRYAG | 1 cut(s) | 235 |
| Sfr303I | CCGCGG | 1 cut(s) | 486 |
| SfuI | TTCGAA | 1 cut(s) | 274 |
| SgrBI | CCGCGG | 1 cut(s) | 486 |
| SinI | GGWCC | 2 cut(s) | 42, 870 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 666, 1040 |
| SmlI | CTYRAG | 2 cut(s) | 68, 266 |
| SmoI | CTYRAG | 2 cut(s) | 68, 266 |
| SnaBI | TACGTA | 1 cut(s) | 682 |
| SpeI | ACTAGT | 1 cut(s) | 715 |
| Sse9I | AATT | 8 cut(s) | 62, 223, 376, 409, 645, 656, 923, 1100 |
| SsiI | CCGC | 6 cut(s) | 483, 485, 555, 839, 843, 1023 |
| SspMI | CTAG | 4 cut(s) | 95, 539, 716, 1016 |
| SstI | GAGCTC | 1 cut(s) | 482 |
| StyD4I | CCNGG | 4 cut(s) | 572, 598, 913, 1144 |
| StyI | CCWWGG | 2 cut(s) | 179, 473 |
| TaaI | ACNGT | 3 cut(s) | 239, 256, 693 |
| TaiI | ACGT | 6 cut(s) | 307, 368, 547, 640, 654, 684 |
| TaqI | TCGA | 4 cut(s) | 99, 194, 274, 368 |
| TasI | AATT | 8 cut(s) | 62, 223, 376, 409, 645, 656, 923, 1100 |
| TauI | GCSGC | 3 cut(s) | 488, 841, 846 |
| TfiI | GAWTC | 9 cut(s) | 101, 111, 215, 382, 421, 465, 611, 833, 1118 |
| Tru1I | TTAA | 7 cut(s) | 198, 336, 444, 654, 758, 926, 935 |
| Tru9I | TTAA | 7 cut(s) | 198, 336, 444, 654, 758, 926, 935 |
| TseFI | GTSAC | 1 cut(s) | 147 |
| TseI | GCWGC | 1 cut(s) | 116 |
| Tsp45I | GTSAC | 1 cut(s) | 147 |
| TspDTI | ATGAA | 4 cut(s) | 75, 593, 624, 650 |
| TspGWI | ACGGA | 1 cut(s) | 744 |
| Tth111I | GACNNNGTC | 1 cut(s) | 254 |
| VpaK11BI | GGWCC | 2 cut(s) | 42, 870 |
| VspI | ATTAAT | 1 cut(s) | 336 |
| XapI | RAATTY | 3 cut(s) | 376, 656, 1100 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 864 |
| XmiI | GTMKAC | 1 cut(s) | 258 |
| XspI | CTAG | 4 cut(s) | 95, 539, 716, 1016 |
| ZraI | GACGTC | 1 cut(s) | 366 |