FvH4_6g16971

Protein of unknown function (DUF295)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000142
Physical Location & Seq
Reverse (-)
10842604 .. 10844300
1697 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g16971.t1

Sequence Viewer

Length: 1359 bp
ATGGGTATCAGTTGCAGTAGAGTTGATGGTAGAATTAATGCAACAAGTTTTGAAGATGACACATTGAAAAATAAACTTTGCCGTCAATTGGAATTAGGAGATGGTGAAAGTGCCCGCCATGGAGTAGTCTCTGTCTCGAATAGGTGGTCTGAACATCTTCCGGATGACTTGATCGAGATGTTGCTGAAGCGGTTATCACTTATTGATTCACAAAAGTTCTCTGCTGTGTGTACATCATGGAGAAGAGCGTCGTCAAAATGGTGTCAACGTGCCACCCTTTTTCCATGGATCTTGAAATGGGTTCGGTCTGTGGACTCGTCAACTGTACTCTGTAGTCTCTATAGTCCAAGTGAAGACAGTGTCTATAATTTTACGTTTCCAAAAGCAACTGCGGATCCTTCGTATTATACATCCTTCCTAGGGTGTATTGATGGATATTTGATCATGAGAGATTATAAAATGCAAAAGTCGACTAGCTACACTCCTTATGGATGGAGCATCAACTACTTGTCGAACCCGATTACAGGTGCTCGGATTATGCTGCCACCCTCCAATGAATCATCCATGATATCTGCAGCCTCCTCAAACCCGAAATCTTCTGACTGCATTGTAGCTACAATACTAACACCACTCACATACAGGTTGGCGTTTTGCAGGCCAGGTGACAAATCATGGACTTACCAATCAGGCCCCAAATATGAAATTCGTTTTGATGAGCTTCAATTCCATAAGGATGGGAAGTTGTACGCATTCGCTAAAGGGGGATATGGTGTTCGATATGTAGTTGTTTTTGATCCCCTAGAGGTTTTTTCAAAAGCCAAAAGTCAGATCTACGTAGGCAAGCAATTTGAAATTGGAAGTTTAATTGCAGGACACATGAATCTGTATGGAATTGAGGCTAATCCTTACTTCATGATAGCTTCTGAAGGTGAAATTTTGCTTGTATGGAATCGGCATAATGGTACAGATAAACACTCCCAACTTCGTCTACAGAATCCAGATGCACCATTTTGGGTGTTCAAGTTAGACCTTGGCCCCGAACCTGCATTGACAGAGACTACTCTTGAAAACCAGACAATGTTCGTTAATCCATATTATAGTTTCTTACGCTTGAACAGACATATCTCGATTCCATTGTCCAAGCTAACCAGTTCAGTGTCAGCGGTATTGAAGGACAAGGCGTTTGATGGAGTCGGAGGTGGAAACTGCATATATTATGCAACAAACACGACTGGTCATTTAGTGATTGGCATCTATAACTTAGCTGATGGAAAAACCGACTATTTTTCAGCAGACAATTACATTGATGGAAATAACGATTCGCCGTATGGACTTGGTTGGTTTCCACTAGTCTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box F-box-like

Protein Analysis

453

Amino Acids

50.75

Weight (kDa)

7.06

Isoelectric Point (pI)

37.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 52 - 85 9.8e-07 F-box domain
Beta-prop_KIB1-4 PF03478 113 - 367 1.8e-23 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000142)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g13532 FvH4_2g28870 FvH4_3g30930 FvH4_3g37281 FvH4_3g37300 FvH4_3g37301 FvH4_3g37301 FvH4_4g23711 FvH4_5g02330 FvH4_6g16960 FvH4_6g16971 FvH4_6g21291 FvH4_6g21331 FvH4_6g22212 FvH4_6g22332 FvH4_6g36920 FvH4_6g37552 FvH4_6g37591 FvH4_6g39721 FvH4_6g46020 FvH4_6g47001 FvH4_6g47002 FvH4_6g47130 FvH4_6g47140 FvH4_6g47161 FvH4_6g47170 FvH4_6g47190 FvH4_6g49120 FvH4_6g49280 FvH4_6g49281 FvH4_6g49640 FvH4_6g49640
malus_domestica MD03G1085600.v1.1 MD09G1027500.v1.1 MD09G1050100.v1.1
prunus_persica Prupe.1G004300_v2.0.a1 Prupe.1G016300_v2.0.a1 Prupe.1G019300_v2.0.a1 Prupe.3G239100_v2.0.a1 Prupe.3G291200_v2.0.a1
pyrus_communis pycom08g02840 pycom08g02860 pycom08g02870
rosa_chinensis RchiOBHm_Chr2g0153831 RchiOBHm_Chr2g0166171 RchiOBHm_Chr2g0166201 RchiOBHm_Chr3g0476221 RchiOBHm_Chr4g0430971 RchiOBHm_Chr5g0067081 RchiOBHm_Chr7g0197821
rosa_laevigata RLG00000001284 RLG00000006996 RLG00000012710 RLG00000019299 RLG00000020285 RLG00000020444 RLG00000020466 RLG00000020591 RLG00000020665 RLG00000020730 RLG00000020732 RLG00000021449 RLG00000021592 RLG00000021593 RLG00000021594 RLG00000021596 RLG00000021597 RLG00000021801 RLG00000023534 RLG00000023538 RLG00000023693 RLG00000023694 RLG00000023699 RLG00000024196 RLG00000024214 RLG00000024220 RLG00000024244 RLG00000024608 RLG00000024612 RLG00000035157 RLG00000035906
rosa_multiflora Rmu_co8282815.1_g000001 Rmu_co8380495.1_g000001 Rmu_co8381111.1_g000001 Rmu_co8460513.1_g000001 Rmu_co8481947.1_g000001 Rmu_co8487937.1_g000001 Rmu_sc0000151.1_g000017 Rmu_sc0001427.1_g000003 Rmu_sc0001427.1_g000006 Rmu_sc0002611.1_g000008 Rmu_sc0003887.1_g000018 Rmu_sc0003887.1_g000019 Rmu_sc0003887.1_g000021 Rmu_sc0004119.1_g000004 Rmu_sc0004329.1_g000003 Rmu_sc0004485.1_g000024 Rmu_sc0005290.1_g000001 Rmu_sc0005609.1_g000006 Rmu_sc0006187.1_g000018 Rmu_sc0006218.1_g000032 Rmu_sc0006218.1_g000033 Rmu_sc0007150.1_g000003 Rmu_sc0007154.1_g000004 Rmu_sc0007154.1_g000005 Rmu_sc0007154.1_g000007 Rmu_sc0008314.1_g000012 Rmu_sc0010073.1_g000023 Rmu_sc0010073.1_g000025 Rmu_sc0011216.1_g000008 Rmu_sc0012445.1_g000005 Rmu_sc0013571.1_g000002 Rmu_sc0018648.1_g000002 Rmu_sc0020961.1_g000002 Rmu_sc0020961.1_g000003 Rmu_sc0023710.1_g000001 Rmu_sc0031310.1_g000008 Rmu_sc0033940.1_g000001 Rmu_sc0035493.1_g000001 Rmu_sc0039285.1_g000001
rosa_roxburghii Rroxscaffold_1G00014000 Rroxscaffold_1G00014020 Rroxscaffold_2G00082760 Rroxscaffold_2G00085090 Rroxscaffold_2G00085100 Rroxscaffold_2G00085140 Rroxscaffold_2G00086670 Rroxscaffold_2G00086680 Rroxscaffold_2G00098190 Rroxscaffold_2G00099270 Rroxscaffold_3G00227460 Rroxscaffold_3G00258190 Rroxscaffold_3G00259780 Rroxscaffold_6G00402940 Rroxscaffold_6G00410200 Rroxscaffold_6G00410210 Rroxscaffold_7G00184320
rosa_rugosa Rorug02G0416100 Rorug02G0416200 Rorug02G0520900 Rorug02G0521200 Rorug02G0521300 Rorug02G0534600 Rorug03G0116300 Rorug03G0162900 Rorug03G0162900 Rorug04G0241400 Rorug05G0380900 Rorug07G0043800
rosa_samantha Rh1AG115300 Rh2BG483700 Rh2BG509900 Rh2BG515700 Rh2BG515800 Rh2BG586500 Rh2BG586600 Rh2BG586700 Rh2BG598800 Rh2BG598900 Rh2BG599100 Rh2BG618400 Rh2BG630500 Rh2CG457400 Rh2CG484900 Rh3AG165300 Rh3AG212900 Rh3AG213300 Rh3BG145800 Rh3BG146100 Rh3BG235300 Rh3BG246500 Rh3BG247000 Rh3BG247100 Rh3CG240300 Rh3CG240800 Rh3CG240900 Rh3CG259600 Rh3DG147000 Rh3DG184800 Rh3DG184900 Rh3DG185000 Rh3DG239500 Rh3DG240100 Rh3DG240200 Rh3DG255800 Rh5BG458500 Rh5CG480400 Rh7BG170400 Rh7BG407900 Rh7CG176900
rosa_wichuraiana Rw2G038990 Rw2G041550 Rw2G047660 Rw2G048940 Rw4G026370 Rw6G008730 Rw7G014600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 456
Acc36I ACCTGC 1 cut(s) 1051
AccI GTMKAC 2 cut(s) 470, 988
AccIII TCCGGA 1 cut(s) 160
AciI CCGC 4 cut(s) 115, 190, 392, 1163
AclWI GGATC 4 cut(s) 296, 389, 402, 788
AcsI RAATTY 2 cut(s) 702, 933
AcuI CTGAAG 2 cut(s) 206, 945
AfaI GTAC 4 cut(s) 232, 327, 746, 964
AfiI CCNNNNNNNGG 3 cut(s) 88, 420, 524
AhlI ACTAGT 1 cut(s) 1348
AjnI CCWGG 1 cut(s) 658
AloI GAACNNNNNNTCC 2 cut(s) 756, 788
AluBI AGCT 6 cut(s) 477, 614, 718, 920, 1144, 1265
AluI AGCT 6 cut(s) 477, 614, 718, 920, 1144, 1265
Alw21I GWGCWC 1 cut(s) 532
Alw26I GTCTC 4 cut(s) 133, 139, 341, 1049
AlwI GGATC 4 cut(s) 296, 389, 402, 788
Aor13HI TCCGGA 1 cut(s) 160
AoxI GGCC 3 cut(s) 656, 688, 1033
ApeKI GCWGC 2 cut(s) 541, 575
ApoI RAATTY 2 cut(s) 702, 933
ArsI GACNNNNNNTTYG 4 cut(s) 131, 163, 1166, 1198
AseI ATTAAT 1 cut(s) 36
Asp700I GAANNNNTTC 1 cut(s) 156
AspA2I CCTAGG 1 cut(s) 418
AspS9I GGNCC 2 cut(s) 689, 1034
AsuHPI GGTGA 3 cut(s) 116, 674, 941
AvrII CCTAGG 1 cut(s) 418
BaeGI GKGCMC 1 cut(s) 115
BamHI GGATCC 1 cut(s) 394
BbsI GAAGAC 1 cut(s) 360
Bbv12I GWGCWC 1 cut(s) 532
BbvI GCAGC 2 cut(s) 528, 587
BccI CCATC 8 cut(s) 20, 95, 425, 486, 728, 1181, 1262, 1301
BceAI ACGGC 2 cut(s) 66, 1309
BciT130I CCWGG 1 cut(s) 660
BclI TGATCA 1 cut(s) 441
BcoDI GTCTC 4 cut(s) 133, 139, 341, 1049
BcuI ACTAGT 1 cut(s) 1348
BfaI CTAG 4 cut(s) 419, 474, 800, 1349
BfmI CTRYAG 4 cut(s) 331, 340, 573, 989
BfuAI ACCTGC 1 cut(s) 1051
BglII AGATCT 1 cut(s) 828
BisI GCNGC 2 cut(s) 542, 576
BlnI CCTAGG 1 cut(s) 418
BlsI GCNGC 2 cut(s) 543, 577
Bme1390I CCNGG 1 cut(s) 660
BmgT120I GGNCC 2 cut(s) 689, 1034
BmiI GGNNCC 3 cut(s) 396, 691, 1036
BmrFI CCNGG 1 cut(s) 660
BmsI GCATC 3 cut(s) 507, 991, 1260
BpiI GAAGAC 1 cut(s) 360
BsaAI YACGTR 1 cut(s) 835
BsaBI GATNNNNATC 1 cut(s) 1250
BsaJI CCNNGG 4 cut(s) 118, 284, 418, 1030
BsaWI WCCGGW 1 cut(s) 160
Bsc4I CCNNNNNNNGG 3 cut(s) 88, 420, 524
Bse1I ACTGG 2 cut(s) 1149, 1237
Bse8I GATNNNNATC 1 cut(s) 1250
BseAI TCCGGA 1 cut(s) 160
BseBI CCWGG 1 cut(s) 660
BseDI CCNNGG 4 cut(s) 118, 284, 418, 1030
BseGI GGATG 5 cut(s) 169, 410, 497, 560, 739
BseJI GATNNNNATC 1 cut(s) 1250
BseLI CCNNNNNNNGG 3 cut(s) 88, 420, 524
BseNI ACTGG 2 cut(s) 1149, 1237
BseRI GAGGAG 1 cut(s) 571
BseSI GKGCMC 1 cut(s) 115
BseXI GCAGC 2 cut(s) 528, 587
BshFI GGCC 3 cut(s) 658, 690, 1035
BsiHKAI GWGCWC 1 cut(s) 532
BsiSI CCGG 1 cut(s) 161
BslI CCNNNNNNNGG 3 cut(s) 88, 420, 524
BsmAI GTCTC 4 cut(s) 133, 139, 341, 1049
BsmI GAATGC 1 cut(s) 749
BsnI GGCC 3 cut(s) 658, 690, 1035
Bsp1286I GDGCHC 2 cut(s) 115, 532
Bsp13I TCCGGA 1 cut(s) 160
Bsp1407I TGTACA 1 cut(s) 230
Bsp143I GATC 6 cut(s) 171, 288, 394, 441, 793, 828
Bsp19I CCATGG 2 cut(s) 118, 284
BspACI CCGC 4 cut(s) 115, 190, 392, 1163
BspANI GGCC 3 cut(s) 658, 690, 1035
BspEI TCCGGA 1 cut(s) 160
BspHI TCATGA 2 cut(s) 444, 912
BspLI GGNNCC 3 cut(s) 396, 691, 1036
BspMAI CTGCAG 1 cut(s) 577
BspMI ACCTGC 1 cut(s) 1051
BspPI GGATC 4 cut(s) 296, 389, 402, 788
BspQI GCTCTTC 1 cut(s) 238
BsrGI TGTACA 1 cut(s) 230
BsrI ACTGG 2 cut(s) 1149, 1237
BssECI CCNNGG 4 cut(s) 118, 284, 418, 1030
BssMI GATC 6 cut(s) 171, 288, 394, 441, 793, 828
BssT1I CCWWGG 4 cut(s) 118, 284, 418, 1030
Bst2UI CCWGG 1 cut(s) 660
Bst4CI ACNGT 2 cut(s) 325, 359
Bst6I CTCTTC 1 cut(s) 238
BstAUI TGTACA 1 cut(s) 230
BstBAI YACGTR 1 cut(s) 835
BstC8I GCNNGC 3 cut(s) 115, 656, 842
BstDEI CTNAG 1 cut(s) 1261
BstDSI CCRYGG 2 cut(s) 118, 284
BstF5I GGATG 5 cut(s) 169, 410, 497, 560, 739
BstKTI GATC 6 cut(s) 174, 291, 397, 444, 796, 831
BstMAI GTCTC 4 cut(s) 133, 139, 341, 1049
BstMBI GATC 6 cut(s) 171, 288, 394, 441, 793, 828
BstNI CCWGG 1 cut(s) 660
BstSCI CCNGG 1 cut(s) 658
BstSFI CTRYAG 4 cut(s) 331, 340, 573, 989
BstSLI GKGCMC 1 cut(s) 115
BstSNI TACGTA 1 cut(s) 835
BstV1I GCAGC 2 cut(s) 528, 587
BstV2I GAAGAC 1 cut(s) 360
BstX2I RGATCY 3 cut(s) 288, 394, 828
BstXI CCANNNNNNTGG 1 cut(s) 734
BstYI RGATCY 3 cut(s) 288, 394, 828
BsuRI GGCC 3 cut(s) 658, 690, 1035
BtgI CCRYGG 2 cut(s) 118, 284
BtsCI GGATG 5 cut(s) 169, 410, 497, 560, 739
BtsIMutI CAGTG 2 cut(s) 364, 1161
BveI ACCTGC 1 cut(s) 1051
Cac8I GCNNGC 3 cut(s) 115, 656, 842
CciI TCATGA 2 cut(s) 444, 912
Cfr13I GGNCC 2 cut(s) 689, 1034
CseI GACGC 1 cut(s) 237
Csp6I GTAC 4 cut(s) 231, 326, 745, 963
CviAII CATG 8 cut(s) 119, 237, 285, 445, 565, 672, 877, 913
CviQI GTAC 4 cut(s) 231, 326, 745, 963
DdeI CTNAG 1 cut(s) 1261
DpnI GATC 6 cut(s) 173, 290, 396, 443, 795, 830
DpnII GATC 6 cut(s) 171, 288, 394, 441, 793, 828
Eam1104I CTCTTC 1 cut(s) 238
EarI CTCTTC 1 cut(s) 238
Eco105I TACGTA 1 cut(s) 835
Eco130I CCWWGG 4 cut(s) 118, 284, 418, 1030
Eco32I GATATC 1 cut(s) 570
Eco57I CTGAAG 2 cut(s) 206, 945
EcoO109I RGGNCCY 1 cut(s) 689
EcoRII CCWGG 1 cut(s) 658
EcoRV GATATC 1 cut(s) 570
EcoT14I CCWWGG 4 cut(s) 118, 284, 418, 1030
ErhI CCWWGG 4 cut(s) 118, 284, 418, 1030
FaeI CATG 8 cut(s) 122, 240, 288, 448, 568, 675, 880, 916
FatI CATG 8 cut(s) 118, 236, 284, 444, 564, 671, 876, 912
FauI CCCGC 1 cut(s) 122
FbaI TGATCA 1 cut(s) 441
FblI GTMKAC 2 cut(s) 470, 988
Fnu4HI GCNGC 2 cut(s) 542, 576
FokI GGATG 5 cut(s) 176, 397, 504, 547, 746
Fsp4HI GCNGC 2 cut(s) 542, 576
FspBI CTAG 4 cut(s) 419, 474, 800, 1349
GluI GCNGC 2 cut(s) 542, 576
HaeIII GGCC 3 cut(s) 658, 690, 1035
HapII CCGG 1 cut(s) 161
HgaI GACGC 1 cut(s) 237
Hin1II CATG 8 cut(s) 122, 240, 288, 448, 568, 675, 880, 916
HincII GTYRAC 3 cut(s) 266, 321, 471
HindII GTYRAC 3 cut(s) 266, 321, 471
HinfI GANTC 9 cut(s) 206, 314, 557, 880, 949, 994, 1129, 1191, 1319
HpaII CCGG 1 cut(s) 161
HphI GGTGA 3 cut(s) 116, 674, 941
Hpy166II GTNNAC 6 cut(s) 231, 266, 313, 321, 471, 989
Hpy188I TCNGA 6 cut(s) 151, 534, 601, 828, 925, 1196
Hpy188III TCNNGA 9 cut(s) 136, 161, 175, 292, 445, 913, 998, 1064, 1126
Hpy8I GTNNAC 6 cut(s) 231, 266, 313, 321, 471, 989
Hpy99I CGWCG 1 cut(s) 253
HpyAV CCTTC 4 cut(s) 408, 424, 920, 1165
HpyCH4III ACNGT 2 cut(s) 325, 359
HpyCH4IV ACGT 3 cut(s) 268, 374, 834
HpyF3I CTNAG 1 cut(s) 1261
HpySE526I ACGT 3 cut(s) 268, 374, 834
Hsp92II CATG 8 cut(s) 122, 240, 288, 448, 568, 675, 880, 916
Kpn2I TCCGGA 1 cut(s) 160
Ksp22I TGATCA 1 cut(s) 441
Kzo9I GATC 6 cut(s) 171, 288, 394, 441, 793, 828
LguI GCTCTTC 1 cut(s) 238
LmnI GCTCC 1 cut(s) 495
Lsp1109I GCAGC 2 cut(s) 528, 587
LweI GCATC 3 cut(s) 507, 991, 1260
MaeI CTAG 4 cut(s) 419, 474, 800, 1349
MaeII ACGT 3 cut(s) 268, 374, 834
MaeIII GTNAC 1 cut(s) 662
MalI GATC 6 cut(s) 173, 290, 396, 443, 795, 830
MboI GATC 6 cut(s) 171, 288, 394, 441, 793, 828
MboII GAAGA 5 cut(s) 65, 149, 255, 365, 588
MfeI CAATTG 1 cut(s) 86
MflI RGATCY 3 cut(s) 288, 394, 828
MhlI GDGCHC 2 cut(s) 115, 532
MlyI GAGTC 2 cut(s) 308, 1200
MmeI TCCRAC 1 cut(s) 1174
MnlI CCTC 6 cut(s) 559, 589, 592, 796, 889, 1190
MroI TCCGGA 1 cut(s) 160
MroXI GAANNNNTTC 1 cut(s) 156
MseI TTAA 4 cut(s) 36, 863, 1086, 1357
MslI CAYNNNNRTG 1 cut(s) 732
MspA1I CMGCKG 1 cut(s) 1163
MspI CCGG 1 cut(s) 161
MspR9I CCNGG 1 cut(s) 660
MunI CAATTG 1 cut(s) 86
Mva1269I GAATGC 1 cut(s) 749
MvaI CCWGG 1 cut(s) 660
NcoI CCATGG 2 cut(s) 118, 284
NdeII GATC 6 cut(s) 171, 288, 394, 441, 793, 828
NlaIII CATG 8 cut(s) 122, 240, 288, 448, 568, 675, 880, 916
NlaIV GGNNCC 3 cut(s) 396, 691, 1036
NmuCI GTSAC 1 cut(s) 662
PagI TCATGA 2 cut(s) 444, 912
PciSI GCTCTTC 1 cut(s) 238
PctI GAATGC 1 cut(s) 749
PdmI GAANNNNTTC 1 cut(s) 156
PfeI GAWTC 7 cut(s) 206, 557, 880, 949, 994, 1129, 1319
PflFI GACNNNGTC 1 cut(s) 359
PkrI GCNGC 2 cut(s) 543, 577
PleI GAGTC 2 cut(s) 308, 1199
PpsI GAGTC 2 cut(s) 308, 1199
Ppu21I YACGTR 1 cut(s) 835
PshBI ATTAAT 1 cut(s) 36
PsiI TTATAA 1 cut(s) 456
Psp6I CCWGG 1 cut(s) 658
PspGI CCWGG 1 cut(s) 658
PspN4I GGNNCC 3 cut(s) 396, 691, 1036
PspPI GGNCC 2 cut(s) 689, 1034
PstI CTGCAG 1 cut(s) 577
PsuI RGATCY 3 cut(s) 288, 394, 828
PsyI GACNNNGTC 1 cut(s) 359
RsaI GTAC 4 cut(s) 232, 327, 746, 964
RsaNI GTAC 4 cut(s) 231, 326, 745, 963
RseI CAYNNNNRTG 1 cut(s) 732
SalI GTCGAC 1 cut(s) 469
SapI GCTCTTC 1 cut(s) 238
SaqAI TTAA 4 cut(s) 36, 863, 1086, 1357
SatI GCNGC 2 cut(s) 542, 576
Sau3AI GATC 6 cut(s) 171, 288, 394, 441, 793, 828
Sau96I GGNCC 2 cut(s) 689, 1034
SchI GAGTC 2 cut(s) 308, 1200
ScrFI CCNGG 1 cut(s) 660
SduI GDGCHC 2 cut(s) 115, 532
SfaNI GCATC 3 cut(s) 507, 991, 1260
SfcI CTRYAG 4 cut(s) 331, 340, 573, 989
SmiMI CAYNNNNRTG 1 cut(s) 732
SnaBI TACGTA 1 cut(s) 835
SpeI ACTAGT 1 cut(s) 1348
SsiI CCGC 4 cut(s) 115, 190, 392, 1163
SspMI CTAG 4 cut(s) 419, 474, 800, 1349
StyD4I CCNGG 1 cut(s) 658
StyI CCWWGG 4 cut(s) 118, 284, 418, 1030
TaaI ACNGT 2 cut(s) 325, 359
TaiI ACGT 3 cut(s) 271, 377, 837
TaqI TCGA 6 cut(s) 137, 174, 470, 512, 775, 1127
TaqII GACCGA 1 cut(s) 294
TatI WGTACW 2 cut(s) 230, 325
TfiI GAWTC 7 cut(s) 206, 557, 880, 949, 994, 1129, 1319
Tru1I TTAA 4 cut(s) 36, 863, 1086, 1357
Tru9I TTAA 4 cut(s) 36, 863, 1086, 1357
TscAI CASTG 2 cut(s) 364, 1161
TseFI GTSAC 1 cut(s) 662
TseI GCWGC 2 cut(s) 541, 575
Tsp45I GTSAC 1 cut(s) 662
TspDTI ATGAA 4 cut(s) 570, 714, 893, 901
TspRI CASTG 2 cut(s) 364, 1161
Tth111I GACNNNGTC 1 cut(s) 359
VspI ATTAAT 1 cut(s) 36
XapI RAATTY 2 cut(s) 702, 933
XmaJI CCTAGG 1 cut(s) 418
XmiI GTMKAC 2 cut(s) 470, 988
XmnI GAANNNNTTC 1 cut(s) 156
XspI CTAG 4 cut(s) 419, 474, 800, 1349
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.