RLG00000021449

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000142
Physical Location & Seq
Reverse (-)
75548011 .. 75549252
1242 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021449

Sequence Viewer

Length: 360 bp
ATGCAATCGATTCTACAGCGGCTATGCATAATAGATTACCTCCTGTGTCGTGAGGTATCTCGTTCTTGGCGAGCTTCTGTTGATAGGGCAATTGTGGTGATAGAGTCATGCTCCCATGCATCACAGACGTCCACAAATTCACACTACGAGAACAATCTTCCTAATTTCTTCGGGAAAGTGGTGGCATCCTCAATACCAACAAAGCAGCACTGTATTGTGGTTAGCCTTTGCTTTGATAAGAAATTGTATTTGTGTAGGCACATTGACAAGTCTTGGACCCTCATTGAGACCGGCTATTGCGATGATATAGAGATAGTCGATGACAAATTATATGCTGCAACGAAGACAGGATCCGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295

Protein Analysis

120

Amino Acids

13.52

Weight (kDa)

6.4

Isoelectric Point (pI)

43.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 53 - 117 1.2e-08 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000142)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g13532 FvH4_2g28870 FvH4_3g30930 FvH4_3g37281 FvH4_3g37300 FvH4_3g37301 FvH4_3g37301 FvH4_4g23711 FvH4_5g02330 FvH4_6g16960 FvH4_6g16971 FvH4_6g21291 FvH4_6g21331 FvH4_6g22212 FvH4_6g22332 FvH4_6g36920 FvH4_6g37552 FvH4_6g37591 FvH4_6g39721 FvH4_6g46020 FvH4_6g47001 FvH4_6g47002 FvH4_6g47130 FvH4_6g47140 FvH4_6g47161 FvH4_6g47170 FvH4_6g47190 FvH4_6g49120 FvH4_6g49280 FvH4_6g49281 FvH4_6g49640 FvH4_6g49640
malus_domestica MD03G1085600.v1.1 MD09G1027500.v1.1 MD09G1050100.v1.1
prunus_persica Prupe.1G004300_v2.0.a1 Prupe.1G016300_v2.0.a1 Prupe.1G019300_v2.0.a1 Prupe.3G239100_v2.0.a1 Prupe.3G291200_v2.0.a1
pyrus_communis pycom08g02840 pycom08g02860 pycom08g02870
rosa_chinensis RchiOBHm_Chr2g0153831 RchiOBHm_Chr2g0166171 RchiOBHm_Chr2g0166201 RchiOBHm_Chr3g0476221 RchiOBHm_Chr4g0430971 RchiOBHm_Chr5g0067081 RchiOBHm_Chr7g0197821
rosa_laevigata RLG00000001284 RLG00000006996 RLG00000012710 RLG00000019299 RLG00000020285 RLG00000020444 RLG00000020466 RLG00000020591 RLG00000020665 RLG00000020730 RLG00000020732 RLG00000021449 RLG00000021592 RLG00000021593 RLG00000021594 RLG00000021596 RLG00000021597 RLG00000021801 RLG00000023534 RLG00000023538 RLG00000023693 RLG00000023694 RLG00000023699 RLG00000024196 RLG00000024214 RLG00000024220 RLG00000024244 RLG00000024608 RLG00000024612 RLG00000035157 RLG00000035906
rosa_multiflora Rmu_co8282815.1_g000001 Rmu_co8380495.1_g000001 Rmu_co8381111.1_g000001 Rmu_co8460513.1_g000001 Rmu_co8481947.1_g000001 Rmu_co8487937.1_g000001 Rmu_sc0000151.1_g000017 Rmu_sc0001427.1_g000003 Rmu_sc0001427.1_g000006 Rmu_sc0002611.1_g000008 Rmu_sc0003887.1_g000018 Rmu_sc0003887.1_g000019 Rmu_sc0003887.1_g000021 Rmu_sc0004119.1_g000004 Rmu_sc0004329.1_g000003 Rmu_sc0004485.1_g000024 Rmu_sc0005290.1_g000001 Rmu_sc0005609.1_g000006 Rmu_sc0006187.1_g000018 Rmu_sc0006218.1_g000032 Rmu_sc0006218.1_g000033 Rmu_sc0007150.1_g000003 Rmu_sc0007154.1_g000004 Rmu_sc0007154.1_g000005 Rmu_sc0007154.1_g000007 Rmu_sc0008314.1_g000012 Rmu_sc0010073.1_g000023 Rmu_sc0010073.1_g000025 Rmu_sc0011216.1_g000008 Rmu_sc0012445.1_g000005 Rmu_sc0013571.1_g000002 Rmu_sc0018648.1_g000002 Rmu_sc0020961.1_g000002 Rmu_sc0020961.1_g000003 Rmu_sc0023710.1_g000001 Rmu_sc0031310.1_g000008 Rmu_sc0033940.1_g000001 Rmu_sc0035493.1_g000001 Rmu_sc0039285.1_g000001
rosa_roxburghii Rroxscaffold_1G00014000 Rroxscaffold_1G00014020 Rroxscaffold_2G00082760 Rroxscaffold_2G00085090 Rroxscaffold_2G00085100 Rroxscaffold_2G00085140 Rroxscaffold_2G00086670 Rroxscaffold_2G00086680 Rroxscaffold_2G00098190 Rroxscaffold_2G00099270 Rroxscaffold_3G00227460 Rroxscaffold_3G00258190 Rroxscaffold_3G00259780 Rroxscaffold_6G00402940 Rroxscaffold_6G00410200 Rroxscaffold_6G00410210 Rroxscaffold_7G00184320
rosa_rugosa Rorug02G0416100 Rorug02G0416200 Rorug02G0520900 Rorug02G0521200 Rorug02G0521300 Rorug02G0534600 Rorug03G0116300 Rorug03G0162900 Rorug03G0162900 Rorug04G0241400 Rorug05G0380900 Rorug07G0043800
rosa_samantha Rh1AG115300 Rh2BG483700 Rh2BG509900 Rh2BG515700 Rh2BG515800 Rh2BG586500 Rh2BG586600 Rh2BG586700 Rh2BG598800 Rh2BG598900 Rh2BG599100 Rh2BG618400 Rh2BG630500 Rh2CG457400 Rh2CG484900 Rh3AG165300 Rh3AG212900 Rh3AG213300 Rh3BG145800 Rh3BG146100 Rh3BG235300 Rh3BG246500 Rh3BG247000 Rh3BG247100 Rh3CG240300 Rh3CG240800 Rh3CG240900 Rh3CG259600 Rh3DG147000 Rh3DG184800 Rh3DG184900 Rh3DG185000 Rh3DG239500 Rh3DG240100 Rh3DG240200 Rh3DG255800 Rh5BG458500 Rh5CG480400 Rh7BG170400 Rh7BG407900 Rh7CG176900
rosa_wichuraiana Rw2G038990 Rw2G041550 Rw2G047660 Rw2G048940 Rw4G026370 Rw6G008730 Rw7G014600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 131
AciI CCGC 1 cut(s) 19
AclWI GGATC 2 cut(s) 345, 358
AcsI RAATTY 1 cut(s) 136
AcyI GRCGYC 1 cut(s) 128
AluBI AGCT 1 cut(s) 74
AluI AGCT 1 cut(s) 74
Alw26I GTCTC 1 cut(s) 281
AlwI GGATC 2 cut(s) 345, 358
ApeKI GCWGC 2 cut(s) 205, 335
ApoI RAATTY 1 cut(s) 136
AspS9I GGNCC 1 cut(s) 276
AsuHPI GGTGA 1 cut(s) 109
AvaII GGWCC 1 cut(s) 276
BamHI GGATCC 1 cut(s) 350
BbsI GAAGAC 1 cut(s) 350
BbvI GCAGC 2 cut(s) 217, 322
BcoDI GTCTC 1 cut(s) 281
BfmI CTRYAG 1 cut(s) 14
BisI GCNGC 3 cut(s) 20, 206, 336
BlsI GCNGC 3 cut(s) 21, 207, 337
Bme18I GGWCC 1 cut(s) 276
BmgT120I GGNCC 1 cut(s) 276
BmiI GGNNCC 2 cut(s) 278, 352
BmsI GCATC 2 cut(s) 128, 194
BpiI GAAGAC 1 cut(s) 350
BplI GAGNNNNNCTC 2 cut(s) 95, 127
Bsa29I ATCGAT 1 cut(s) 8
BsaHI GRCGYC 1 cut(s) 128
BsaI GGTCTC 1 cut(s) 281
Bse118I RCCGGY 1 cut(s) 290
BseCI ATCGAT 1 cut(s) 8
BseGI GGATG 1 cut(s) 185
BseXI GCAGC 2 cut(s) 217, 322
BshVI ATCGAT 1 cut(s) 8
BsiSI CCGG 1 cut(s) 291
BsmAI GTCTC 1 cut(s) 281
Bso31I GGTCTC 1 cut(s) 281
Bsp143I GATC 1 cut(s) 350
BspACI CCGC 1 cut(s) 19
BspDI ATCGAT 1 cut(s) 8
BspLI GGNNCC 2 cut(s) 278, 352
BspPI GGATC 2 cut(s) 345, 358
BspTNI GGTCTC 1 cut(s) 281
BsrFI RCCGGY 1 cut(s) 290
BssAI RCCGGY 1 cut(s) 290
BssMI GATC 1 cut(s) 350
BssNI GRCGYC 1 cut(s) 128
Bst4CI ACNGT 1 cut(s) 212
BstACI GRCGYC 1 cut(s) 128
BstC8I GCNNGC 1 cut(s) 72
BstF5I GGATG 1 cut(s) 185
BstKTI GATC 1 cut(s) 353
BstMAI GTCTC 1 cut(s) 281
BstMBI GATC 1 cut(s) 350
BstSFI CTRYAG 1 cut(s) 14
BstV1I GCAGC 2 cut(s) 217, 322
BstV2I GAAGAC 1 cut(s) 350
BstX2I RGATCY 1 cut(s) 350
BstYI RGATCY 1 cut(s) 350
Bsu15I ATCGAT 1 cut(s) 8
BsuTUI ATCGAT 1 cut(s) 8
BtgZI GCGATG 1 cut(s) 315
BtsCI GGATG 1 cut(s) 185
BtsIMutI CAGTG 1 cut(s) 208
Cac8I GCNNGC 1 cut(s) 72
Cfr10I RCCGGY 1 cut(s) 290
Cfr13I GGNCC 1 cut(s) 276
ClaI ATCGAT 1 cut(s) 8
CviAII CATG 2 cut(s) 108, 116
CviJI RGCY 4 cut(s) 22, 74, 225, 294
CviKI_1 RGCY 4 cut(s) 22, 74, 225, 294
DpnI GATC 1 cut(s) 352
DpnII GATC 1 cut(s) 350
Eco31I GGTCTC 1 cut(s) 281
Eco47I GGWCC 1 cut(s) 276
EcoT22I ATGCAT 2 cut(s) 29, 121
FaeI CATG 2 cut(s) 111, 119
FaiI YATR 7 cut(s) 25, 29, 109, 117, 308, 331, 333
FatI CATG 2 cut(s) 107, 115
Fnu4HI GCNGC 3 cut(s) 20, 206, 336
FokI GGATG 1 cut(s) 172
Fsp4HI GCNGC 3 cut(s) 20, 206, 336
GluI GCNGC 3 cut(s) 20, 206, 336
HapII CCGG 1 cut(s) 291
Hin1I GRCGYC 1 cut(s) 128
Hin1II CATG 2 cut(s) 111, 119
HinfI GANTC 2 cut(s) 10, 104
HpaII CCGG 1 cut(s) 291
HphI GGTGA 1 cut(s) 109
Hpy166II GTNNAC 1 cut(s) 132
Hpy188I TCNGA 1 cut(s) 355
Hpy188III TCNNGA 2 cut(s) 50, 172
Hpy8I GTNNAC 1 cut(s) 132
HpyCH4III ACNGT 1 cut(s) 212
HpyCH4IV ACGT 1 cut(s) 128
HpyCH4V TGCA 4 cut(s) 4, 27, 119, 338
HpySE526I ACGT 1 cut(s) 128
Hsp92I GRCGYC 1 cut(s) 128
Hsp92II CATG 2 cut(s) 111, 119
Kzo9I GATC 1 cut(s) 350
LmnI GCTCC 1 cut(s) 116
LpnPI CCDG 3 cut(s) 56, 304, 333
Lsp1109I GCAGC 2 cut(s) 217, 322
LweI GCATC 2 cut(s) 128, 194
MaeII ACGT 1 cut(s) 128
MalI GATC 1 cut(s) 352
MboI GATC 1 cut(s) 350
MboII GAAGA 3 cut(s) 149, 160, 355
MfeI CAATTG 1 cut(s) 90
MflI RGATCY 1 cut(s) 350
MluCI AATT 5 cut(s) 90, 136, 163, 242, 326
MlyI GAGTC 1 cut(s) 113
MnlI CCTC 4 cut(s) 46, 50, 199, 290
Mph1103I ATGCAT 2 cut(s) 29, 121
MseI TTAA 1 cut(s) 358
MspA1I CMGCKG 1 cut(s) 19
MspI CCGG 1 cut(s) 291
MunI CAATTG 1 cut(s) 90
NdeII GATC 1 cut(s) 350
NlaIII CATG 2 cut(s) 111, 119
NlaIV GGNNCC 2 cut(s) 278, 352
NsiI ATGCAT 2 cut(s) 29, 121
PcsI WCGNNNNNNNCGW 1 cut(s) 67
PfeI GAWTC 1 cut(s) 10
PkrI GCNGC 3 cut(s) 21, 207, 337
PleI GAGTC 1 cut(s) 112
PpsI GAGTC 1 cut(s) 112
PspN4I GGNNCC 2 cut(s) 278, 352
PspPI GGNCC 1 cut(s) 276
PsuI RGATCY 1 cut(s) 350
SaqAI TTAA 1 cut(s) 358
SatI GCNGC 3 cut(s) 20, 206, 336
Sau3AI GATC 1 cut(s) 350
Sau96I GGNCC 1 cut(s) 276
SchI GAGTC 1 cut(s) 113
SetI ASST 4 cut(s) 42, 57, 76, 131
SfaNI GCATC 2 cut(s) 128, 194
SfcI CTRYAG 1 cut(s) 14
SinI GGWCC 1 cut(s) 276
Sse9I AATT 5 cut(s) 90, 136, 163, 242, 326
SsiI CCGC 1 cut(s) 19
TaaI ACNGT 1 cut(s) 212
TaiI ACGT 1 cut(s) 131
TaqI TCGA 2 cut(s) 8, 318
TasI AATT 5 cut(s) 90, 136, 163, 242, 326
TauI GCSGC 1 cut(s) 22
TfiI GAWTC 1 cut(s) 10
Tru1I TTAA 1 cut(s) 358
Tru9I TTAA 1 cut(s) 358
TscAI CASTG 1 cut(s) 215
TseI GCWGC 2 cut(s) 205, 335
TspRI CASTG 1 cut(s) 215
VpaK11BI GGWCC 1 cut(s) 276
XapI RAATTY 1 cut(s) 136
ZraI GACGTC 1 cut(s) 129
Zsp2I ATGCAT 2 cut(s) 29, 121
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.