FvH4_6g22212

F-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000142
Physical Location & Seq
Forward (+)
15886828 .. 15888594
1767 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g22212.t1

Sequence Viewer

Length: 1317 bp
ATGTTCTGCAGATGGTCAGACCTTCTCCAAGGCCTAGGCCTCTTCTCTTCCAGTGCATGCGCTGGTCTTTTATCGAGTATGAGTCACGAAAGACAACAGCAGGTGGATGACAACAACAGTAGGTGGTCGAAGCACCTTCCTGATGAATTGATCGAGATGATTGTGAAGCGTCTAACCCTAGTCGATTCAATCAGATTCGCTGCTGTTTGTTCTACTTGGAGAAGTGTATCATCACAATGGTTTCAACGAGTAACCAGACTTCCATGGCTCTTAAAATCGGTTGAGATTCTAGACTCAAAAACTACTGTGAATTATAGTCTCTATAGTGTAAGTGAAGACACAGCCTACGACCTCAAGTTTCCAAGCGAAAGCGCCTCCAGTGACGGGATACATTGCACGGGCTCTGCTAATGGCTGGTTGATAATGTCTGAATATTGTAATGAACCGATAACGTGCACCAATTATTTATTGAACCCAATGACAGGTGATCGTATTAAGCTTCCCCAAGTGACCGGATTCATCCCTCAAGTGTCCACGGCCTCTTCAGATCCAAGATCTCCTGATTGCATTGTTGCAACTAAGAACGGTTTAGGGTTCATCTTTTGCAGGCCAGGTAGTCAAGACTGGAGTTGGACGACAACGACACCGGAGCTTGGTAGGATGGATTCCATGAAATTTCATGATGATGGTAAGTTATACGTAACAGGACGGAAGTATGATGCGTATGATGTGGTCCGAAGGTTTAGCGATTATTATCATGTGCTTGTTGTTTTTGTTCCTGTTATTGGCAAAGACGAGGAAATTACTTGGGACAAGGAAAGGGTGATAAAATTAGCACCCCACGAGCCTTGGACCGGTGTGGAGTCAATAACCGAACCGTGGCTGGAGTTCGCGGGTCCAGAAGTTACTTTGTCCTCCAAAGGTAAGCTTTTGCTAGTTTGGAGGCGAAGGTACATTCACGAGCCCGACCCGCCACACTTTCCATATCATGTGGTAAAGATGGATGATGATGGCTGGCCAGGCTGGGAGAACTCGGTGGATTTTCATGTGGTAAAGAAAGATAAGATTTGGGCAGCGTATGATGCTTCTGAAGACGAACTAGTCTTGATATCTCATGGCTACATCTCAGTGCGAAAGTCCAATAAGTCATTTACGCGGCCAACCATAGACGAGCGCTTTTTTGGACGTCATTGCAGAAAACTGAGCACATTCTGTTGGGAGACTACGGAAGATGGTGGTCTGTTGGATAATATTAAATATGGGCGATGGTTTTCGCCCAACATAGCAGCGCTGCCAAATAATTCAAGGAGATTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box F-box-like

Protein Analysis

439

Amino Acids

50.25

Weight (kDa)

6.08

Isoelectric Point (pI)

51.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 45 - 77 3e-07 F-box domain
Beta-prop_KIB1-4 PF03478 107 - 384 1.3e-23 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000142)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g13532 FvH4_2g28870 FvH4_3g30930 FvH4_3g37281 FvH4_3g37300 FvH4_3g37301 FvH4_3g37301 FvH4_4g23711 FvH4_5g02330 FvH4_6g16960 FvH4_6g16971 FvH4_6g21291 FvH4_6g21331 FvH4_6g22212 FvH4_6g22332 FvH4_6g36920 FvH4_6g37552 FvH4_6g37591 FvH4_6g39721 FvH4_6g46020 FvH4_6g47001 FvH4_6g47002 FvH4_6g47130 FvH4_6g47140 FvH4_6g47161 FvH4_6g47170 FvH4_6g47190 FvH4_6g49120 FvH4_6g49280 FvH4_6g49281 FvH4_6g49640 FvH4_6g49640
malus_domestica MD03G1085600.v1.1 MD09G1027500.v1.1 MD09G1050100.v1.1
prunus_persica Prupe.1G004300_v2.0.a1 Prupe.1G016300_v2.0.a1 Prupe.1G019300_v2.0.a1 Prupe.3G239100_v2.0.a1 Prupe.3G291200_v2.0.a1
pyrus_communis pycom08g02840 pycom08g02860 pycom08g02870
rosa_chinensis RchiOBHm_Chr2g0153831 RchiOBHm_Chr2g0166171 RchiOBHm_Chr2g0166201 RchiOBHm_Chr3g0476221 RchiOBHm_Chr4g0430971 RchiOBHm_Chr5g0067081 RchiOBHm_Chr7g0197821
rosa_laevigata RLG00000001284 RLG00000006996 RLG00000012710 RLG00000019299 RLG00000020285 RLG00000020444 RLG00000020466 RLG00000020591 RLG00000020665 RLG00000020730 RLG00000020732 RLG00000021449 RLG00000021592 RLG00000021593 RLG00000021594 RLG00000021596 RLG00000021597 RLG00000021801 RLG00000023534 RLG00000023538 RLG00000023693 RLG00000023694 RLG00000023699 RLG00000024196 RLG00000024214 RLG00000024220 RLG00000024244 RLG00000024608 RLG00000024612 RLG00000035157 RLG00000035906
rosa_multiflora Rmu_co8282815.1_g000001 Rmu_co8380495.1_g000001 Rmu_co8381111.1_g000001 Rmu_co8460513.1_g000001 Rmu_co8481947.1_g000001 Rmu_co8487937.1_g000001 Rmu_sc0000151.1_g000017 Rmu_sc0001427.1_g000003 Rmu_sc0001427.1_g000006 Rmu_sc0002611.1_g000008 Rmu_sc0003887.1_g000018 Rmu_sc0003887.1_g000019 Rmu_sc0003887.1_g000021 Rmu_sc0004119.1_g000004 Rmu_sc0004329.1_g000003 Rmu_sc0004485.1_g000024 Rmu_sc0005290.1_g000001 Rmu_sc0005609.1_g000006 Rmu_sc0006187.1_g000018 Rmu_sc0006218.1_g000032 Rmu_sc0006218.1_g000033 Rmu_sc0007150.1_g000003 Rmu_sc0007154.1_g000004 Rmu_sc0007154.1_g000005 Rmu_sc0007154.1_g000007 Rmu_sc0008314.1_g000012 Rmu_sc0010073.1_g000023 Rmu_sc0010073.1_g000025 Rmu_sc0011216.1_g000008 Rmu_sc0012445.1_g000005 Rmu_sc0013571.1_g000002 Rmu_sc0018648.1_g000002 Rmu_sc0020961.1_g000002 Rmu_sc0020961.1_g000003 Rmu_sc0023710.1_g000001 Rmu_sc0031310.1_g000008 Rmu_sc0033940.1_g000001 Rmu_sc0035493.1_g000001 Rmu_sc0039285.1_g000001
rosa_roxburghii Rroxscaffold_1G00014000 Rroxscaffold_1G00014020 Rroxscaffold_2G00082760 Rroxscaffold_2G00085090 Rroxscaffold_2G00085100 Rroxscaffold_2G00085140 Rroxscaffold_2G00086670 Rroxscaffold_2G00086680 Rroxscaffold_2G00098190 Rroxscaffold_2G00099270 Rroxscaffold_3G00227460 Rroxscaffold_3G00258190 Rroxscaffold_3G00259780 Rroxscaffold_6G00402940 Rroxscaffold_6G00410200 Rroxscaffold_6G00410210 Rroxscaffold_7G00184320
rosa_rugosa Rorug02G0416100 Rorug02G0416200 Rorug02G0520900 Rorug02G0521200 Rorug02G0521300 Rorug02G0534600 Rorug03G0116300 Rorug03G0162900 Rorug03G0162900 Rorug04G0241400 Rorug05G0380900 Rorug07G0043800
rosa_samantha Rh1AG115300 Rh2BG483700 Rh2BG509900 Rh2BG515700 Rh2BG515800 Rh2BG586500 Rh2BG586600 Rh2BG586700 Rh2BG598800 Rh2BG598900 Rh2BG599100 Rh2BG618400 Rh2BG630500 Rh2CG457400 Rh2CG484900 Rh3AG165300 Rh3AG212900 Rh3AG213300 Rh3BG145800 Rh3BG146100 Rh3BG235300 Rh3BG246500 Rh3BG247000 Rh3BG247100 Rh3CG240300 Rh3CG240800 Rh3CG240900 Rh3CG259600 Rh3DG147000 Rh3DG184800 Rh3DG184900 Rh3DG185000 Rh3DG239500 Rh3DG240100 Rh3DG240200 Rh3DG255800 Rh5BG458500 Rh5CG480400 Rh7BG170400 Rh7BG407900 Rh7CG176900
rosa_wichuraiana Rw2G038990 Rw2G041550 Rw2G047660 Rw2G048940 Rw4G026370 Rw6G008730 Rw7G014600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 91
AasI GACNNNNNNGTC 1 cut(s) 1100
AatII GACGTC 1 cut(s) 1189
Acc36I ACCTGC 1 cut(s) 91
AccII CGCG 2 cut(s) 893, 1156
AciI CCGC 3 cut(s) 893, 971, 1156
AclWI GGATC 1 cut(s) 542
AcoI YGGCCR 2 cut(s) 1016, 1157
AcsI RAATTY 1 cut(s) 674
AcuI CTGAAG 2 cut(s) 528, 1110
AcyI GRCGYC 1 cut(s) 1186
AfaI GTAC 1 cut(s) 953
AfeI AGCGCT 2 cut(s) 1175, 1290
AfiI CCNNNNNNNGG 6 cut(s) 384, 482, 653, 785, 854, 879
AgeI ACCGGT 1 cut(s) 854
AgsI TTSAA 4 cut(s) 189, 245, 472, 1305
AhlI ACTAGT 1 cut(s) 1099
AjnI CCWGG 2 cut(s) 610, 1018
AluBI AGCT 3 cut(s) 499, 652, 928
AluI AGCT 3 cut(s) 499, 652, 928
Alw21I GWGCWC 2 cut(s) 458, 1208
Alw26I GTCTC 2 cut(s) 323, 1214
Alw44I GTGCAC 1 cut(s) 454
AlwI GGATC 1 cut(s) 542
Aor51HI AGCGCT 2 cut(s) 1175, 1290
AoxI GGCC 6 cut(s) 31, 37, 537, 608, 1016, 1157
ApaLI GTGCAC 1 cut(s) 454
ApeKI GCWGC 4 cut(s) 200, 1073, 1286, 1291
ApoI RAATTY 1 cut(s) 674
AsiGI ACCGGT 1 cut(s) 854
AspA2I CCTAGG 1 cut(s) 34
AspLEI GCGC 4 cut(s) 62, 374, 1176, 1291
AspS9I GGNCC 3 cut(s) 733, 852, 896
AsuHPI GGTGA 2 cut(s) 497, 835
AvaII GGWCC 3 cut(s) 733, 852, 896
AvrII CCTAGG 1 cut(s) 34
BaeGI GKGCMC 1 cut(s) 458
BalI TGGCCA 1 cut(s) 1018
BanII GRGCYC 2 cut(s) 404, 966
BarI GAAGNNNNNNTAC 2 cut(s) 243, 275
BauI CACGAG 2 cut(s) 842, 959
BbsI GAAGAC 2 cut(s) 342, 1098
Bbv12I GWGCWC 2 cut(s) 458, 1208
BbvI GCAGC 4 cut(s) 187, 1085, 1278, 1298
BccI CCATC 7 cut(s) 6, 655, 680, 994, 1004, 1226, 1260
BceAI ACGGC 1 cut(s) 552
BciT130I CCWGG 2 cut(s) 612, 1020
BciVI GTATCC 1 cut(s) 381
BcoDI GTCTC 2 cut(s) 323, 1214
BcuI ACTAGT 1 cut(s) 1099
BfaI CTAG 6 cut(s) 35, 179, 290, 935, 1100, 1315
BfmI CTRYAG 2 cut(s) 7, 322
BfoI RGCGCY 3 cut(s) 375, 1177, 1292
BfuAI ACCTGC 1 cut(s) 91
BfuI GTATCC 1 cut(s) 381
BglII AGATCT 1 cut(s) 554
BisI GCNGC 5 cut(s) 201, 1074, 1157, 1287, 1292
BlnI CCTAGG 1 cut(s) 34
BlsI GCNGC 5 cut(s) 202, 1075, 1158, 1288, 1293
Bme1390I CCNGG 2 cut(s) 612, 1020
Bme18I GGWCC 3 cut(s) 733, 852, 896
BmgT120I GGNCC 3 cut(s) 733, 852, 896
BmiI GGNNCC 1 cut(s) 897
BmrFI CCNGG 2 cut(s) 612, 1020
BmsI GCATC 2 cut(s) 709, 1072
BpiI GAAGAC 2 cut(s) 342, 1098
BpmI CTGGAG 3 cut(s) 361, 646, 905
BpuEI CTTGAG 2 cut(s) 338, 510
BsaAI YACGTR 1 cut(s) 700
BsaBI GATNNNNATC 1 cut(s) 753
BsaHI GRCGYC 1 cut(s) 1186
BsaJI CCNNGG 6 cut(s) 28, 34, 263, 534, 848, 878
BsaWI WCCGGW 3 cut(s) 512, 646, 854
Bsc4I CCNNNNNNNGG 6 cut(s) 384, 482, 653, 785, 854, 879
Bse118I RCCGGY 1 cut(s) 854
Bse1I ACTGG 3 cut(s) 51, 378, 629
Bse3DI GCAATG 2 cut(s) 391, 1189
Bse8I GATNNNNATC 1 cut(s) 753
BseBI CCWGG 2 cut(s) 612, 1020
BseDI CCNNGG 6 cut(s) 28, 34, 263, 534, 848, 878
BseGI GGATG 4 cut(s) 112, 519, 666, 1009
BseJI GATNNNNATC 1 cut(s) 753
BseLI CCNNNNNNNGG 6 cut(s) 384, 482, 653, 785, 854, 879
BseMI GCAATG 2 cut(s) 391, 1189
BseMII CTCAG 2 cut(s) 1140, 1193
BseNI ACTGG 3 cut(s) 51, 378, 629
BseSI GKGCMC 1 cut(s) 458
BseXI GCAGC 4 cut(s) 187, 1085, 1278, 1298
BseYI CCCAGC 1 cut(s) 1023
Bsh1236I CGCG 2 cut(s) 893, 1156
BshFI GGCC 6 cut(s) 33, 39, 539, 610, 1018, 1159
BshTI ACCGGT 1 cut(s) 854
BsiHKAI GWGCWC 2 cut(s) 458, 1208
BsiSI CCGG 3 cut(s) 513, 647, 855
BslFI GGGAC 1 cut(s) 824
BslI CCNNNNNNNGG 6 cut(s) 384, 482, 653, 785, 854, 879
BsmAI GTCTC 2 cut(s) 323, 1214
BsmFI GGGAC 1 cut(s) 824
BsnI GGCC 6 cut(s) 33, 39, 539, 610, 1018, 1159
Bsp1286I GDGCHC 4 cut(s) 404, 458, 966, 1208
Bsp143I GATC 4 cut(s) 150, 487, 547, 554
Bsp19I CCATGG 1 cut(s) 263
BspACI CCGC 3 cut(s) 893, 971, 1156
BspANI GGCC 6 cut(s) 33, 39, 539, 610, 1018, 1159
BspCNI CTCAG 2 cut(s) 1139, 1194
BspFNI CGCG 2 cut(s) 893, 1156
BspHI TCATGA 1 cut(s) 679
BspLI GGNNCC 1 cut(s) 897
BspMAI CTGCAG 1 cut(s) 11
BspMI ACCTGC 1 cut(s) 91
BspPI GGATC 1 cut(s) 542
BsrDI GCAATG 2 cut(s) 391, 1189
BsrFI RCCGGY 1 cut(s) 854
BsrI ACTGG 3 cut(s) 51, 378, 629
BssAI RCCGGY 1 cut(s) 854
BssECI CCNNGG 6 cut(s) 28, 34, 263, 534, 848, 878
BssMI GATC 4 cut(s) 150, 487, 547, 554
BssNI GRCGYC 1 cut(s) 1186
BssSI CACGAG 2 cut(s) 842, 959
BssT1I CCWWGG 4 cut(s) 28, 34, 263, 848
Bst2BI CACGAG 2 cut(s) 842, 959
Bst2UI CCWGG 2 cut(s) 612, 1020
Bst4CI ACNGT 4 cut(s) 119, 307, 587, 879
Bst6I CTCTTC 3 cut(s) 47, 52, 547
BstACI GRCGYC 1 cut(s) 1186
BstBAI YACGTR 1 cut(s) 700
BstC8I GCNNGC 3 cut(s) 58, 608, 1016
BstDEI CTNAG 3 cut(s) 579, 1126, 1202
BstDSI CCRYGG 3 cut(s) 263, 534, 878
BstF5I GGATG 4 cut(s) 112, 519, 666, 1009
BstFNI CGCG 2 cut(s) 893, 1156
BstH2I RGCGCY 3 cut(s) 375, 1177, 1292
BstHHI GCGC 4 cut(s) 62, 374, 1176, 1291
BstKTI GATC 4 cut(s) 153, 490, 550, 557
BstMAI GTCTC 2 cut(s) 323, 1214
BstMBI GATC 4 cut(s) 150, 487, 547, 554
BstMWI GCNNNNNNNGC 3 cut(s) 970, 1020, 1082
BstNI CCWGG 2 cut(s) 612, 1020
BstNSI RCATGY 1 cut(s) 60
BstSCI CCNGG 2 cut(s) 610, 1018
BstSFI CTRYAG 2 cut(s) 7, 322
BstSLI GKGCMC 1 cut(s) 458
BstSNI TACGTA 1 cut(s) 700
BstUI CGCG 2 cut(s) 893, 1156
BstV1I GCAGC 4 cut(s) 187, 1085, 1278, 1298
BstV2I GAAGAC 2 cut(s) 342, 1098
BstX2I RGATCY 2 cut(s) 547, 554
BstYI RGATCY 2 cut(s) 547, 554
BsuI GTATCC 1 cut(s) 381
BsuRI GGCC 6 cut(s) 33, 39, 539, 610, 1018, 1159
BtgI CCRYGG 3 cut(s) 263, 534, 878
BtgZI GCGATG 1 cut(s) 1279
BtsCI GGATG 4 cut(s) 112, 519, 666, 1009
BtsIMutI CAGTG 3 cut(s) 58, 385, 1134
BveI ACCTGC 1 cut(s) 91
Cac8I GCNNGC 3 cut(s) 58, 608, 1016
CciI TCATGA 1 cut(s) 679
CfoI GCGC 4 cut(s) 62, 374, 1176, 1291
Cfr10I RCCGGY 1 cut(s) 854
Cfr13I GGNCC 3 cut(s) 733, 852, 896
CseI GACGC 1 cut(s) 158
Csp6I GTAC 1 cut(s) 952
CspAI ACCGGT 1 cut(s) 854
CspCI CAANNNNNGTGG 2 cut(s) 830, 865
CviAII CATG 8 cut(s) 57, 264, 670, 680, 758, 989, 1046, 1115
CviQI GTAC 1 cut(s) 952
DdeI CTNAG 3 cut(s) 579, 1126, 1202
DpnI GATC 4 cut(s) 152, 489, 549, 556
DpnII GATC 4 cut(s) 150, 487, 547, 554
DrdI GACNNNNNNGTC 1 cut(s) 1100
DseDI GACNNNNNNGTC 1 cut(s) 1100
EaeI YGGCCR 2 cut(s) 1016, 1157
Eam1104I CTCTTC 3 cut(s) 47, 52, 547
EarI CTCTTC 3 cut(s) 47, 52, 547
Eco105I TACGTA 1 cut(s) 700
Eco130I CCWWGG 4 cut(s) 28, 34, 263, 848
Eco147I AGGCCT 2 cut(s) 33, 39
Eco24I GRGCYC 2 cut(s) 404, 966
Eco32I GATATC 1 cut(s) 1110
Eco47I GGWCC 3 cut(s) 733, 852, 896
Eco47III AGCGCT 2 cut(s) 1175, 1290
Eco57I CTGAAG 2 cut(s) 528, 1110
EcoRII CCWGG 2 cut(s) 610, 1018
EcoRV GATATC 1 cut(s) 1110
EcoT14I CCWWGG 4 cut(s) 28, 34, 263, 848
EcoT38I GRGCYC 2 cut(s) 404, 966
ErhI CCWWGG 4 cut(s) 28, 34, 263, 848
FaeI CATG 8 cut(s) 60, 267, 673, 683, 761, 992, 1049, 1118
FalI AAGNNNNNCTT 2 cut(s) 912, 944
FaqI GGGAC 1 cut(s) 824
FatI CATG 8 cut(s) 56, 263, 669, 679, 757, 988, 1045, 1114
FauI CCCGC 2 cut(s) 886, 978
Fnu4HI GCNGC 5 cut(s) 201, 1074, 1157, 1287, 1292
FokI GGATG 4 cut(s) 119, 506, 673, 1016
FriOI GRGCYC 2 cut(s) 404, 966
Fsp4HI GCNGC 5 cut(s) 201, 1074, 1157, 1287, 1292
FspBI CTAG 6 cut(s) 35, 179, 290, 935, 1100, 1315
GlaI GCGC 4 cut(s) 61, 373, 1175, 1290
GluI GCNGC 5 cut(s) 201, 1074, 1157, 1287, 1292
GsaI CCCAGC 1 cut(s) 1027
GsuI CTGGAG 3 cut(s) 361, 646, 905
HaeII RGCGCY 3 cut(s) 375, 1177, 1292
HaeIII GGCC 6 cut(s) 33, 39, 539, 610, 1018, 1159
HapII CCGG 3 cut(s) 513, 647, 855
HgaI GACGC 1 cut(s) 158
HhaI GCGC 4 cut(s) 62, 374, 1176, 1291
Hin1I GRCGYC 1 cut(s) 1186
Hin1II CATG 8 cut(s) 60, 267, 673, 683, 761, 992, 1049, 1118
Hin6I GCGC 4 cut(s) 60, 372, 1174, 1289
HinP1I GCGC 4 cut(s) 60, 372, 1174, 1289
HindIII AAGCTT 2 cut(s) 497, 926
HinfI GANTC 9 cut(s) 82, 185, 195, 286, 293, 516, 665, 863, 1311
HpaII CCGG 3 cut(s) 513, 647, 855
HphI GGTGA 2 cut(s) 497, 835
Hpy166II GTNNAC 2 cut(s) 456, 534
Hpy188I TCNGA 6 cut(s) 19, 194, 430, 547, 737, 1090
Hpy8I GTNNAC 2 cut(s) 456, 534
HpyAV CCTTC 4 cut(s) 32, 146, 732, 942
HpyCH4III ACNGT 4 cut(s) 119, 307, 587, 879
HpyCH4IV ACGT 3 cut(s) 452, 699, 1186
HpyCH4V TGCA 8 cut(s) 9, 56, 396, 456, 567, 575, 606, 1194
HpyF10VI GCNNNNNNNGC 3 cut(s) 970, 1020, 1082
HpyF3I CTNAG 3 cut(s) 579, 1126, 1202
HpySE526I ACGT 3 cut(s) 452, 699, 1186
Hsp92I GRCGYC 1 cut(s) 1186
Hsp92II CATG 8 cut(s) 60, 267, 673, 683, 761, 992, 1049, 1118
HspAI GCGC 4 cut(s) 60, 372, 1174, 1289
Kzo9I GATC 4 cut(s) 150, 487, 547, 554
LmnI GCTCC 1 cut(s) 649
Lsp1109I GCAGC 4 cut(s) 187, 1085, 1278, 1298
LweI GCATC 2 cut(s) 709, 1072
MaeI CTAG 6 cut(s) 35, 179, 290, 935, 1100, 1315
MaeII ACGT 3 cut(s) 452, 699, 1186
MaeIII GTNAC 6 cut(s) 83, 250, 380, 508, 700, 904
MalI GATC 4 cut(s) 152, 489, 549, 556
MboI GATC 4 cut(s) 150, 487, 547, 554
MboII GAAGA 6 cut(s) 34, 39, 347, 534, 1103, 1241
MflI RGATCY 2 cut(s) 547, 554
MhlI GDGCHC 4 cut(s) 404, 458, 966, 1208
MlsI TGGCCA 1 cut(s) 1018
MluCI AATT 7 cut(s) 146, 310, 460, 674, 801, 830, 1300
MluNI TGGCCA 1 cut(s) 1018
MlyI GAGTC 3 cut(s) 91, 287, 872
MmeI TCCRAC 2 cut(s) 611, 1224
MnlI CCTC 8 cut(s) 50, 362, 385, 534, 550, 790, 925, 936
Mox20I TGGCCA 1 cut(s) 1018
MscI TGGCCA 1 cut(s) 1018
MseI TTAA 3 cut(s) 272, 495, 1254
MslI CAYNNNNRTG 3 cut(s) 235, 684, 1127
Msp20I TGGCCA 1 cut(s) 1018
MspI CCGG 3 cut(s) 513, 647, 855
MspR9I CCNGG 2 cut(s) 612, 1020
MvaI CCWGG 2 cut(s) 612, 1020
MvnI CGCG 2 cut(s) 893, 1156
MwoI GCNNNNNNNGC 3 cut(s) 970, 1020, 1082
NcoI CCATGG 1 cut(s) 263
NdeII GATC 4 cut(s) 150, 487, 547, 554
NlaIII CATG 8 cut(s) 60, 267, 673, 683, 761, 992, 1049, 1118
NlaIV GGNNCC 1 cut(s) 897
NmuCI GTSAC 3 cut(s) 83, 380, 508
NspI RCATGY 1 cut(s) 60
PaeI GCATGC 1 cut(s) 60
PagI TCATGA 1 cut(s) 679
PaqCI CACCTGC 1 cut(s) 91
PceI AGGCCT 2 cut(s) 33, 39
PfeI GAWTC 6 cut(s) 185, 195, 286, 516, 665, 1311
PinAI ACCGGT 1 cut(s) 854
PkrI GCNGC 5 cut(s) 202, 1075, 1158, 1288, 1293
PleI GAGTC 3 cut(s) 90, 287, 871
PpsI GAGTC 3 cut(s) 90, 287, 871
Ppu21I YACGTR 1 cut(s) 700
Psp6I CCWGG 2 cut(s) 610, 1018
PspFI CCCAGC 1 cut(s) 1023
PspGI CCWGG 2 cut(s) 610, 1018
PspN4I GGNNCC 1 cut(s) 897
PspPI GGNCC 3 cut(s) 733, 852, 896
PstI CTGCAG 1 cut(s) 11
PsuI RGATCY 2 cut(s) 547, 554
RsaI GTAC 1 cut(s) 953
RsaNI GTAC 1 cut(s) 952
RseI CAYNNNNRTG 3 cut(s) 235, 684, 1127
SaqAI TTAA 3 cut(s) 272, 495, 1254
SatI GCNGC 5 cut(s) 201, 1074, 1157, 1287, 1292
Sau3AI GATC 4 cut(s) 150, 487, 547, 554
Sau96I GGNCC 3 cut(s) 733, 852, 896
SchI GAGTC 3 cut(s) 91, 287, 872
ScrFI CCNGG 2 cut(s) 612, 1020
SduI GDGCHC 4 cut(s) 404, 458, 966, 1208
SfaNI GCATC 2 cut(s) 709, 1072
SfcI CTRYAG 2 cut(s) 7, 322
SinI GGWCC 3 cut(s) 733, 852, 896
SmiMI CAYNNNNRTG 3 cut(s) 235, 684, 1127
SmlI CTYRAG 2 cut(s) 353, 525
SmoI CTYRAG 2 cut(s) 353, 525
SnaBI TACGTA 1 cut(s) 700
SpeI ACTAGT 1 cut(s) 1099
SphI GCATGC 1 cut(s) 60
Sse9I AATT 7 cut(s) 146, 310, 460, 674, 801, 830, 1300
SseBI AGGCCT 2 cut(s) 33, 39
SsiI CCGC 3 cut(s) 893, 971, 1156
SspI AATATT 2 cut(s) 434, 1252
SspMI CTAG 6 cut(s) 35, 179, 290, 935, 1100, 1315
StuI AGGCCT 2 cut(s) 33, 39
StyD4I CCNGG 2 cut(s) 610, 1018
StyI CCWWGG 4 cut(s) 28, 34, 263, 848
TaaI ACNGT 4 cut(s) 119, 307, 587, 879
TaiI ACGT 3 cut(s) 455, 702, 1189
TaqI TCGA 4 cut(s) 74, 128, 153, 183
TasI AATT 7 cut(s) 146, 310, 460, 674, 801, 830, 1300
TauI GCSGC 1 cut(s) 1159
TfiI GAWTC 6 cut(s) 185, 195, 286, 516, 665, 1311
Tru1I TTAA 3 cut(s) 272, 495, 1254
Tru9I TTAA 3 cut(s) 272, 495, 1254
TscAI CASTG 3 cut(s) 58, 385, 1134
TseFI GTSAC 3 cut(s) 83, 380, 508
TseI GCWGC 4 cut(s) 200, 1073, 1286, 1291
Tsp45I GTSAC 3 cut(s) 83, 380, 508
TspDTI ATGAA 7 cut(s) 159, 456, 508, 586, 668, 686, 1034
TspGWI ACGGA 2 cut(s) 724, 1241
TspRI CASTG 3 cut(s) 58, 385, 1134
VneI GTGCAC 1 cut(s) 454
VpaK11BI GGWCC 3 cut(s) 733, 852, 896
XapI RAATTY 1 cut(s) 674
XbaI TCTAGA 1 cut(s) 289
XceI RCATGY 1 cut(s) 60
XmaJI CCTAGG 1 cut(s) 34
XspI CTAG 6 cut(s) 35, 179, 290, 935, 1100, 1315
ZraI GACGTC 1 cut(s) 1187
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.