Rroxscaffold_2G00099270

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000142
Physical Location & Seq
Reverse (-)
20899207 .. 20900065
859 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00099270.1

Sequence Viewer

Length: 759 bp
ATGCTACCATCAGAACTCACCATTCCCCCCCAGGATGGTAGTATTGATGGTGGACTATCATTCTTGCTTAAAGTAGTAGCCTCTTCGGTACCAACGAGCCCGCACTGCCTTGTGGCTAGTTATTATTCCTGTGGTCGTTTGGCTTTTTGTAGACCCTCCGATAAATCATGGACCATCATTGACGCTGATGAGGTGGTTTTTGGAGGGTTCGGAAAAGAGATTAGCGGTATCGAAATAGTTGAGGAGAAATTATACGTCTTAAGGATGAATGCACCGAGAGTTTTATTCGTGTTTGACATCCACATAGATGCCAATGGCGCCGGTGCTCATACCTATACTACTGTGAGGTCAGTTATGCATTACCCTTGGCCAGTTCCTTCCCGCCCTACATGGGATGGACGCATGCATGTGAGTAATTCTGAACCAGTGACTTACATATTCAAAGACTGTACATCAAAAGAGTTGTTTATGACTTTTGTAATTAGTAAGAGTTATTACATTATTGACGAGCCGCTGAAGCGTGCGATCGATGTTCCTCGTACTAAGGGATTTCGAGTGTTCAAGCTAGAGCATAATAACTGTCCTCGATGGGTAGAAGTAACCGACCTTGGTAATCGGATCGATATTGTTTGCGGGCATGATCTTGGTGTGTATTCTTTGACAGATGGGAGCATCCAACGTTACACGTTTCCTTTCGCGAAGGATCACTGTTCTCATGAATGGTCTCTGCCTATCTGGTTTACACCAAATCTGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295

Protein Analysis

252

Amino Acids

28.51

Weight (kDa)

6.3

Isoelectric Point (pI)

44.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 20 - 207 1e-16 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000142)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g13532 FvH4_2g28870 FvH4_3g30930 FvH4_3g37281 FvH4_3g37300 FvH4_3g37301 FvH4_3g37301 FvH4_4g23711 FvH4_5g02330 FvH4_6g16960 FvH4_6g16971 FvH4_6g21291 FvH4_6g21331 FvH4_6g22212 FvH4_6g22332 FvH4_6g36920 FvH4_6g37552 FvH4_6g37591 FvH4_6g39721 FvH4_6g46020 FvH4_6g47001 FvH4_6g47002 FvH4_6g47130 FvH4_6g47140 FvH4_6g47161 FvH4_6g47170 FvH4_6g47190 FvH4_6g49120 FvH4_6g49280 FvH4_6g49281 FvH4_6g49640 FvH4_6g49640
malus_domestica MD03G1085600.v1.1 MD09G1027500.v1.1 MD09G1050100.v1.1
prunus_persica Prupe.1G004300_v2.0.a1 Prupe.1G016300_v2.0.a1 Prupe.1G019300_v2.0.a1 Prupe.3G239100_v2.0.a1 Prupe.3G291200_v2.0.a1
pyrus_communis pycom08g02840 pycom08g02860 pycom08g02870
rosa_chinensis RchiOBHm_Chr2g0153831 RchiOBHm_Chr2g0166171 RchiOBHm_Chr2g0166201 RchiOBHm_Chr3g0476221 RchiOBHm_Chr4g0430971 RchiOBHm_Chr5g0067081 RchiOBHm_Chr7g0197821
rosa_laevigata RLG00000001284 RLG00000006996 RLG00000012710 RLG00000019299 RLG00000020285 RLG00000020444 RLG00000020466 RLG00000020591 RLG00000020665 RLG00000020730 RLG00000020732 RLG00000021449 RLG00000021592 RLG00000021593 RLG00000021594 RLG00000021596 RLG00000021597 RLG00000021801 RLG00000023534 RLG00000023538 RLG00000023693 RLG00000023694 RLG00000023699 RLG00000024196 RLG00000024214 RLG00000024220 RLG00000024244 RLG00000024608 RLG00000024612 RLG00000035157 RLG00000035906
rosa_multiflora Rmu_co8282815.1_g000001 Rmu_co8380495.1_g000001 Rmu_co8381111.1_g000001 Rmu_co8460513.1_g000001 Rmu_co8481947.1_g000001 Rmu_co8487937.1_g000001 Rmu_sc0000151.1_g000017 Rmu_sc0001427.1_g000003 Rmu_sc0001427.1_g000006 Rmu_sc0002611.1_g000008 Rmu_sc0003887.1_g000018 Rmu_sc0003887.1_g000019 Rmu_sc0003887.1_g000021 Rmu_sc0004119.1_g000004 Rmu_sc0004329.1_g000003 Rmu_sc0004485.1_g000024 Rmu_sc0005290.1_g000001 Rmu_sc0005609.1_g000006 Rmu_sc0006187.1_g000018 Rmu_sc0006218.1_g000032 Rmu_sc0006218.1_g000033 Rmu_sc0007150.1_g000003 Rmu_sc0007154.1_g000004 Rmu_sc0007154.1_g000005 Rmu_sc0007154.1_g000007 Rmu_sc0008314.1_g000012 Rmu_sc0010073.1_g000023 Rmu_sc0010073.1_g000025 Rmu_sc0011216.1_g000008 Rmu_sc0012445.1_g000005 Rmu_sc0013571.1_g000002 Rmu_sc0018648.1_g000002 Rmu_sc0020961.1_g000002 Rmu_sc0020961.1_g000003 Rmu_sc0023710.1_g000001 Rmu_sc0031310.1_g000008 Rmu_sc0033940.1_g000001 Rmu_sc0035493.1_g000001 Rmu_sc0039285.1_g000001
rosa_roxburghii Rroxscaffold_1G00014000 Rroxscaffold_1G00014020 Rroxscaffold_2G00082760 Rroxscaffold_2G00085090 Rroxscaffold_2G00085100 Rroxscaffold_2G00085140 Rroxscaffold_2G00086670 Rroxscaffold_2G00086680 Rroxscaffold_2G00098190 Rroxscaffold_2G00099270 Rroxscaffold_3G00227460 Rroxscaffold_3G00258190 Rroxscaffold_3G00259780 Rroxscaffold_6G00402940 Rroxscaffold_6G00410200 Rroxscaffold_6G00410210 Rroxscaffold_7G00184320
rosa_rugosa Rorug02G0416100 Rorug02G0416200 Rorug02G0520900 Rorug02G0521200 Rorug02G0521300 Rorug02G0534600 Rorug03G0116300 Rorug03G0162900 Rorug03G0162900 Rorug04G0241400 Rorug05G0380900 Rorug07G0043800
rosa_samantha Rh1AG115300 Rh2BG483700 Rh2BG509900 Rh2BG515700 Rh2BG515800 Rh2BG586500 Rh2BG586600 Rh2BG586700 Rh2BG598800 Rh2BG598900 Rh2BG599100 Rh2BG618400 Rh2BG630500 Rh2CG457400 Rh2CG484900 Rh3AG165300 Rh3AG212900 Rh3AG213300 Rh3BG145800 Rh3BG146100 Rh3BG235300 Rh3BG246500 Rh3BG247000 Rh3BG247100 Rh3CG240300 Rh3CG240800 Rh3CG240900 Rh3CG259600 Rh3DG147000 Rh3DG184800 Rh3DG184900 Rh3DG185000 Rh3DG239500 Rh3DG240100 Rh3DG240200 Rh3DG255800 Rh5BG458500 Rh5CG480400 Rh7BG170400 Rh7BG407900 Rh7CG176900
rosa_wichuraiana Rw2G038990 Rw2G041550 Rw2G047660 Rw2G048940 Rw4G026370 Rw6G008730 Rw7G014600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 88
AccB1I GGYRCC 2 cut(s) 88, 317
AccI GTMKAC 1 cut(s) 151
AccII CGCG 1 cut(s) 698
AciI CCGC 5 cut(s) 101, 225, 382, 512, 633
AclI AACGTT 1 cut(s) 679
AclWI GGATC 2 cut(s) 626, 711
AcoI YGGCCR 1 cut(s) 368
AcuI CTGAAG 1 cut(s) 536
AcyI GRCGYC 1 cut(s) 318
AfaI GTAC 3 cut(s) 90, 451, 541
AfiI CCNNNNNNNGG 2 cut(s) 35, 391
AflII CTTAAG 1 cut(s) 259
AflIII ACRYGT 1 cut(s) 684
AgsI TTSAA 2 cut(s) 442, 562
AjnI CCWGG 1 cut(s) 30
AluBI AGCT 1 cut(s) 565
AluI AGCT 1 cut(s) 565
Alw21I GWGCWC 1 cut(s) 328
Alw26I GTCTC 1 cut(s) 729
AlwI GGATC 2 cut(s) 626, 711
AoxI GGCC 1 cut(s) 368
Asp718I GGTACC 1 cut(s) 88
AspLEI GCGC 1 cut(s) 320
AspS9I GGNCC 1 cut(s) 171
AsuHPI GGTGA 1 cut(s) 10
AvaII GGWCC 1 cut(s) 171
BalI TGGCCA 1 cut(s) 370
BanI GGYRCC 2 cut(s) 88, 317
BanII GRGCYC 1 cut(s) 101
Bbv12I GWGCWC 1 cut(s) 328
BccI CCATC 7 cut(s) 16, 29, 41, 182, 389, 582, 659
BciT130I CCWGG 1 cut(s) 32
BcoDI GTCTC 1 cut(s) 729
BfaI CTAG 2 cut(s) 117, 566
BfoI RGCGCY 1 cut(s) 321
BfrI CTTAAG 1 cut(s) 259
BisI GCNGC 1 cut(s) 512
BlsI GCNGC 1 cut(s) 513
Bme1390I CCNGG 1 cut(s) 32
Bme18I GGWCC 1 cut(s) 171
BmgT120I GGNCC 1 cut(s) 171
BmiI GGNNCC 2 cut(s) 90, 319
BmrFI CCNGG 1 cut(s) 32
BmsI GCATC 2 cut(s) 298, 681
Bsa29I ATCGAT 2 cut(s) 528, 621
BsaHI GRCGYC 1 cut(s) 318
BsaI GGTCTC 1 cut(s) 729
BsaJI CCNNGG 3 cut(s) 30, 365, 607
Bsc4I CCNNNNNNNGG 2 cut(s) 35, 391
Bse118I RCCGGY 1 cut(s) 320
Bse1I ACTGG 2 cut(s) 371, 425
BseBI CCWGG 1 cut(s) 32
BseCI ATCGAT 2 cut(s) 528, 621
BseDI CCNNGG 3 cut(s) 30, 365, 607
BseGI GGATG 5 cut(s) 40, 270, 297, 400, 672
BseLI CCNNNNNNNGG 2 cut(s) 35, 391
BseNI ACTGG 2 cut(s) 371, 425
BseRI GAGGAG 1 cut(s) 257
Bsh1236I CGCG 1 cut(s) 698
Bsh1285I CGRYCG 1 cut(s) 528
BshFI GGCC 1 cut(s) 370
BshNI GGYRCC 2 cut(s) 88, 317
BshVI ATCGAT 2 cut(s) 528, 621
BsiEI CGRYCG 1 cut(s) 528
BsiHKAI GWGCWC 1 cut(s) 328
BsiSI CCGG 1 cut(s) 321
BslI CCNNNNNNNGG 2 cut(s) 35, 391
BsmAI GTCTC 1 cut(s) 729
BsmI GAATGC 1 cut(s) 274
BsnI GGCC 1 cut(s) 370
Bso31I GGTCTC 1 cut(s) 729
Bsp1286I GDGCHC 2 cut(s) 101, 328
Bsp1407I TGTACA 1 cut(s) 449
Bsp143I GATC 4 cut(s) 525, 618, 640, 703
Bsp68I TCGCGA 1 cut(s) 698
BspACI CCGC 5 cut(s) 101, 225, 382, 512, 633
BspANI GGCC 1 cut(s) 370
BspDI ATCGAT 2 cut(s) 528, 621
BspFNI CGCG 1 cut(s) 698
BspHI TCATGA 1 cut(s) 715
BspLI GGNNCC 2 cut(s) 90, 319
BspPI GGATC 2 cut(s) 626, 711
BspT107I GGYRCC 2 cut(s) 88, 317
BspTI CTTAAG 1 cut(s) 259
BspTNI GGTCTC 1 cut(s) 729
BsrFI RCCGGY 1 cut(s) 320
BsrGI TGTACA 1 cut(s) 449
BsrI ACTGG 2 cut(s) 371, 425
BssAI RCCGGY 1 cut(s) 320
BssECI CCNNGG 3 cut(s) 30, 365, 607
BssMI GATC 4 cut(s) 525, 618, 640, 703
BssNI GRCGYC 1 cut(s) 318
BssT1I CCWWGG 2 cut(s) 365, 607
Bst2UI CCWGG 1 cut(s) 32
Bst4CI ACNGT 4 cut(s) 343, 449, 581, 710
Bst6I CTCTTC 1 cut(s) 88
BstACI GRCGYC 1 cut(s) 318
BstAFI CTTAAG 1 cut(s) 259
BstAUI TGTACA 1 cut(s) 449
BstC8I GCNNGC 4 cut(s) 101, 404, 522, 635
BstDEI CTNAG 1 cut(s) 543
BstF5I GGATG 5 cut(s) 40, 270, 297, 400, 672
BstFNI CGCG 1 cut(s) 698
BstH2I RGCGCY 1 cut(s) 321
BstHHI GCGC 1 cut(s) 320
BstKTI GATC 4 cut(s) 528, 621, 643, 706
BstMAI GTCTC 1 cut(s) 729
BstMBI GATC 4 cut(s) 525, 618, 640, 703
BstMCI CGRYCG 1 cut(s) 528
BstMWI GCNNNNNNNGC 3 cut(s) 105, 317, 517
BstNI CCWGG 1 cut(s) 32
BstNSI RCATGY 2 cut(s) 406, 410
BstSCI CCNGG 1 cut(s) 30
BstUI CGCG 1 cut(s) 698
Bsu15I ATCGAT 2 cut(s) 528, 621
BsuRI GGCC 1 cut(s) 370
BsuTUI ATCGAT 2 cut(s) 528, 621
BtsCI GGATG 5 cut(s) 40, 270, 297, 400, 672
BtsI GCAGTG 1 cut(s) 103
BtsIMutI CAGTG 3 cut(s) 103, 432, 706
BtuMI TCGCGA 1 cut(s) 698
Cac8I GCNNGC 4 cut(s) 101, 404, 522, 635
CciI TCATGA 1 cut(s) 715
CfoI GCGC 1 cut(s) 320
Cfr10I RCCGGY 1 cut(s) 320
Cfr13I GGNCC 1 cut(s) 171
ClaI ATCGAT 2 cut(s) 528, 621
CseI GACGC 2 cut(s) 191, 408
Csp6I GTAC 3 cut(s) 89, 450, 540
CviAII CATG 6 cut(s) 168, 390, 403, 407, 638, 716
CviJI RGCY 7 cut(s) 80, 99, 116, 143, 370, 511, 565
CviKI_1 RGCY 7 cut(s) 80, 99, 116, 143, 370, 511, 565
CviQI GTAC 3 cut(s) 89, 450, 540
DdeI CTNAG 1 cut(s) 543
DinI GGCGCC 1 cut(s) 319
DpnI GATC 4 cut(s) 527, 620, 642, 705
DpnII GATC 4 cut(s) 525, 618, 640, 703
EaeI YGGCCR 1 cut(s) 368
Eam1104I CTCTTC 1 cut(s) 88
EarI CTCTTC 1 cut(s) 88
Eco130I CCWWGG 2 cut(s) 365, 607
Eco24I GRGCYC 1 cut(s) 101
Eco31I GGTCTC 1 cut(s) 729
Eco47I GGWCC 1 cut(s) 171
Eco57I CTGAAG 1 cut(s) 536
EcoRII CCWGG 1 cut(s) 30
EcoT14I CCWWGG 2 cut(s) 365, 607
EcoT22I ATGCAT 2 cut(s) 360, 408
EcoT38I GRGCYC 1 cut(s) 101
EgeI GGCGCC 1 cut(s) 319
EheI GGCGCC 1 cut(s) 319
ErhI CCWWGG 2 cut(s) 365, 607
FaeI CATG 6 cut(s) 171, 393, 406, 410, 641, 719
FatI CATG 6 cut(s) 167, 389, 402, 406, 637, 715
FauI CCCGC 3 cut(s) 108, 389, 626
FblI GTMKAC 1 cut(s) 151
Fnu4HI GCNGC 1 cut(s) 512
FokI GGATG 5 cut(s) 47, 277, 284, 407, 659
FriOI GRGCYC 1 cut(s) 101
Fsp4HI GCNGC 1 cut(s) 512
FspBI CTAG 2 cut(s) 117, 566
GlaI GCGC 1 cut(s) 319
GluI GCNGC 1 cut(s) 512
HaeII RGCGCY 1 cut(s) 321
HaeIII GGCC 1 cut(s) 370
HapII CCGG 1 cut(s) 321
HgaI GACGC 2 cut(s) 191, 408
HhaI GCGC 1 cut(s) 320
Hin1I GRCGYC 1 cut(s) 318
Hin1II CATG 6 cut(s) 171, 393, 406, 410, 641, 719
Hin6I GCGC 1 cut(s) 318
HinP1I GCGC 1 cut(s) 318
HpaII CCGG 1 cut(s) 321
HphI GGTGA 1 cut(s) 10
Hpy166II GTNNAC 3 cut(s) 53, 152, 741
Hpy188I TCNGA 6 cut(s) 13, 160, 212, 421, 618, 753
Hpy188III TCNNGA 2 cut(s) 697, 716
Hpy8I GTNNAC 3 cut(s) 53, 152, 741
HpyAV CCTTC 2 cut(s) 387, 694
HpyCH4III ACNGT 4 cut(s) 343, 449, 581, 710
HpyCH4IV ACGT 3 cut(s) 255, 679, 686
HpyCH4V TGCA 3 cut(s) 272, 358, 406
HpyF10VI GCNNNNNNNGC 3 cut(s) 105, 317, 517
HpyF3I CTNAG 1 cut(s) 543
HpySE526I ACGT 3 cut(s) 255, 679, 686
Hsp92I GRCGYC 1 cut(s) 318
Hsp92II CATG 6 cut(s) 171, 393, 406, 410, 641, 719
HspAI GCGC 1 cut(s) 318
KasI GGCGCC 1 cut(s) 317
KpnI GGTACC 1 cut(s) 92
Kzo9I GATC 4 cut(s) 525, 618, 640, 703
LmnI GCTCC 1 cut(s) 669
LpnPI CCDG 7 cut(s) 17, 44, 142, 334, 384, 438, 721
LweI GCATC 2 cut(s) 298, 681
MaeI CTAG 2 cut(s) 117, 566
MaeII ACGT 3 cut(s) 255, 679, 686
MaeIII GTNAC 3 cut(s) 427, 598, 680
MalI GATC 4 cut(s) 527, 620, 642, 705
MboI GATC 4 cut(s) 525, 618, 640, 703
MboII GAAGA 1 cut(s) 75
MhlI GDGCHC 2 cut(s) 101, 328
MlsI TGGCCA 1 cut(s) 370
MluCI AATT 3 cut(s) 248, 415, 480
MluNI TGGCCA 1 cut(s) 370
Mly113I GGCGCC 1 cut(s) 318
MmeI TCCRAC 1 cut(s) 700
MnlI CCTC 8 cut(s) 91, 166, 184, 197, 235, 339, 546, 594
Mox20I TGGCCA 1 cut(s) 370
Mph1103I ATGCAT 2 cut(s) 360, 408
MscI TGGCCA 1 cut(s) 370
MseI TTAA 2 cut(s) 69, 260
MslI CAYNNNNRTG 2 cut(s) 306, 407
Msp20I TGGCCA 1 cut(s) 370
MspA1I CMGCKG 1 cut(s) 514
MspCI CTTAAG 1 cut(s) 259
MspI CCGG 1 cut(s) 321
MspR9I CCNGG 1 cut(s) 32
Mva1269I GAATGC 1 cut(s) 274
MvaI CCWGG 1 cut(s) 32
MvnI CGCG 1 cut(s) 698
MwoI GCNNNNNNNGC 3 cut(s) 105, 317, 517
NarI GGCGCC 1 cut(s) 318
NdeII GATC 4 cut(s) 525, 618, 640, 703
NlaIII CATG 6 cut(s) 171, 393, 406, 410, 641, 719
NlaIV GGNNCC 2 cut(s) 90, 319
NmuCI GTSAC 1 cut(s) 427
NruI TCGCGA 1 cut(s) 698
NsiI ATGCAT 2 cut(s) 360, 408
NspI RCATGY 2 cut(s) 406, 410
PaeI GCATGC 1 cut(s) 406
PagI TCATGA 1 cut(s) 715
PcsI WCGNNNNNNNCGW 1 cut(s) 92
PctI GAATGC 1 cut(s) 274
PkrI GCNGC 1 cut(s) 513
Ple19I CGATCG 1 cut(s) 528
PluTI GGCGCC 1 cut(s) 321
Psp1406I AACGTT 1 cut(s) 679
Psp6I CCWGG 1 cut(s) 30
PspGI CCWGG 1 cut(s) 30
PspN4I GGNNCC 2 cut(s) 90, 319
PspPI GGNCC 1 cut(s) 171
PvuI CGATCG 1 cut(s) 528
RruI TCGCGA 1 cut(s) 698
RsaI GTAC 3 cut(s) 90, 451, 541
RsaNI GTAC 3 cut(s) 89, 450, 540
RseI CAYNNNNRTG 2 cut(s) 306, 407
SaqAI TTAA 2 cut(s) 69, 260
SatI GCNGC 1 cut(s) 512
Sau3AI GATC 4 cut(s) 525, 618, 640, 703
Sau96I GGNCC 1 cut(s) 171
ScrFI CCNGG 1 cut(s) 32
SduI GDGCHC 2 cut(s) 101, 328
SetI ASST 8 cut(s) 195, 258, 335, 350, 567, 609, 682, 689
SfaNI GCATC 2 cut(s) 298, 681
SfoI GGCGCC 1 cut(s) 319
SgrAI CRCCGGYG 1 cut(s) 320
SinI GGWCC 1 cut(s) 171
SmiMI CAYNNNNRTG 2 cut(s) 306, 407
SmlI CTYRAG 1 cut(s) 259
SmoI CTYRAG 1 cut(s) 259
SphI GCATGC 1 cut(s) 406
Sse9I AATT 3 cut(s) 248, 415, 480
SsiI CCGC 5 cut(s) 101, 225, 382, 512, 633
SspDI GGCGCC 1 cut(s) 317
SspMI CTAG 2 cut(s) 117, 566
StyD4I CCNGG 1 cut(s) 30
StyI CCWWGG 2 cut(s) 365, 607
TaaI ACNGT 4 cut(s) 343, 449, 581, 710
TaiI ACGT 3 cut(s) 258, 682, 689
TaqI TCGA 5 cut(s) 231, 528, 553, 586, 621
TasI AATT 3 cut(s) 248, 415, 480
TatI WGTACW 1 cut(s) 449
TauI GCSGC 1 cut(s) 514
Tru1I TTAA 2 cut(s) 69, 260
Tru9I TTAA 2 cut(s) 69, 260
TscAI CASTG 3 cut(s) 110, 432, 713
TseFI GTSAC 1 cut(s) 427
Tsp45I GTSAC 1 cut(s) 427
TspDTI ATGAA 2 cut(s) 281, 732
TspRI CASTG 3 cut(s) 110, 432, 713
Vha464I CTTAAG 1 cut(s) 259
VpaK11BI GGWCC 1 cut(s) 171
XceI RCATGY 2 cut(s) 406, 410
XmiI GTMKAC 1 cut(s) 151
XspI CTAG 2 cut(s) 117, 566
Zsp2I ATGCAT 2 cut(s) 360, 408
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.