Required for maturation of ribosomal RNAs and formation of the large ribosomal subunit
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 457 |
| AclWI | GGATC | 1 cut(s) | 848 |
| AcsI | RAATTY | 3 cut(s) | 67, 145, 780 |
| AcyI | GRCGYC | 1 cut(s) | 243 |
| AfaI | GTAC | 3 cut(s) | 300, 437, 819 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 337 |
| AflIII | ACRYGT | 1 cut(s) | 707 |
| AgsI | TTSAA | 6 cut(s) | 327, 742, 752, 898, 915, 1060 |
| AjnI | CCWGG | 4 cut(s) | 265, 399, 446, 654 |
| AluBI | AGCT | 3 cut(s) | 163, 424, 541 |
| AluI | AGCT | 3 cut(s) | 163, 424, 541 |
| Alw26I | GTCTC | 1 cut(s) | 1045 |
| AlwI | GGATC | 1 cut(s) | 848 |
| AoxI | GGCC | 2 cut(s) | 499, 648 |
| ApeKI | GCWGC | 3 cut(s) | 389, 424, 592 |
| ApoI | RAATTY | 3 cut(s) | 67, 145, 780 |
| AseI | ATTAAT | 1 cut(s) | 932 |
| AspS9I | GGNCC | 4 cut(s) | 397, 500, 649, 937 |
| AsuHPI | GGTGA | 1 cut(s) | 979 |
| AvaII | GGWCC | 2 cut(s) | 397, 937 |
| BanII | GRGCYC | 1 cut(s) | 692 |
| BbvI | GCAGC | 3 cut(s) | 401, 411, 579 |
| BccI | CCATC | 5 cut(s) | 112, 202, 366, 727, 912 |
| BciT130I | CCWGG | 4 cut(s) | 267, 401, 448, 656 |
| BclI | TGATCA | 2 cut(s) | 218, 528 |
| BcoDI | GTCTC | 1 cut(s) | 1045 |
| BfaI | CTAG | 2 cut(s) | 542, 772 |
| BfmI | CTRYAG | 3 cut(s) | 387, 660, 813 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 656 |
| BisI | GCNGC | 3 cut(s) | 390, 425, 593 |
| BlsI | GCNGC | 3 cut(s) | 391, 426, 594 |
| BmcAI | AGTACT | 2 cut(s) | 300, 819 |
| Bme1390I | CCNGG | 4 cut(s) | 267, 401, 448, 656 |
| Bme18I | GGWCC | 2 cut(s) | 397, 937 |
| BmgT120I | GGNCC | 4 cut(s) | 397, 500, 649, 937 |
| BmrFI | CCNGG | 4 cut(s) | 267, 401, 448, 656 |
| BmrI | ACTGGG | 1 cut(s) | 686 |
| BmsI | GCATC | 1 cut(s) | 616 |
| BmuI | ACTGGG | 1 cut(s) | 686 |
| BpmI | CTGGAG | 1 cut(s) | 853 |
| BpuEI | CTTGAG | 1 cut(s) | 706 |
| BsaHI | GRCGYC | 1 cut(s) | 243 |
| BsaI | GGTCTC | 1 cut(s) | 1045 |
| BsaJI | CCNNGG | 4 cut(s) | 73, 265, 850, 963 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 337 |
| Bse1I | ACTGG | 3 cut(s) | 296, 692, 697 |
| Bse3DI | GCAATG | 2 cut(s) | 399, 651 |
| BseBI | CCWGG | 4 cut(s) | 267, 401, 448, 656 |
| BseDI | CCNNGG | 4 cut(s) | 73, 265, 850, 963 |
| BseGI | GGATG | 2 cut(s) | 93, 607 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 337 |
| BseMI | GCAATG | 2 cut(s) | 399, 651 |
| BseMII | CTCAG | 2 cut(s) | 239, 955 |
| BseNI | ACTGG | 3 cut(s) | 296, 692, 697 |
| BseRI | GAGGAG | 2 cut(s) | 808, 850 |
| BseXI | GCAGC | 3 cut(s) | 401, 411, 579 |
| BshFI | GGCC | 2 cut(s) | 501, 650 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 337 |
| BsmAI | GTCTC | 1 cut(s) | 1045 |
| BsnI | GGCC | 2 cut(s) | 501, 650 |
| Bso31I | GGTCTC | 1 cut(s) | 1045 |
| Bsp1286I | GDGCHC | 1 cut(s) | 692 |
| Bsp143I | GATC | 5 cut(s) | 218, 528, 840, 860, 1007 |
| Bsp19I | CCATGG | 1 cut(s) | 73 |
| BspANI | GGCC | 2 cut(s) | 501, 650 |
| BspCNI | CTCAG | 2 cut(s) | 240, 954 |
| BspMAI | CTGCAG | 2 cut(s) | 391, 817 |
| BspPI | GGATC | 1 cut(s) | 848 |
| BspTNI | GGTCTC | 1 cut(s) | 1045 |
| BsrDI | GCAATG | 2 cut(s) | 399, 651 |
| BsrI | ACTGG | 3 cut(s) | 296, 692, 697 |
| BssECI | CCNNGG | 4 cut(s) | 73, 265, 850, 963 |
| BssMI | GATC | 5 cut(s) | 218, 528, 840, 860, 1007 |
| BssNI | GRCGYC | 1 cut(s) | 243 |
| BssT1I | CCWWGG | 2 cut(s) | 73, 850 |
| Bst2UI | CCWGG | 4 cut(s) | 267, 401, 448, 656 |
| Bst4CI | ACNGT | 3 cut(s) | 24, 454, 1069 |
| Bst6I | CTCTTC | 1 cut(s) | 175 |
| BstACI | GRCGYC | 1 cut(s) | 243 |
| BstC8I | GCNNGC | 2 cut(s) | 17, 42 |
| BstDEI | CTNAG | 3 cut(s) | 124, 248, 941 |
| BstDSI | CCRYGG | 1 cut(s) | 73 |
| BstEII | GGTNACC | 1 cut(s) | 967 |
| BstF5I | GGATG | 2 cut(s) | 93, 607 |
| BstKTI | GATC | 5 cut(s) | 221, 531, 843, 863, 1010 |
| BstMAI | GTCTC | 1 cut(s) | 1045 |
| BstMBI | GATC | 5 cut(s) | 218, 528, 840, 860, 1007 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 656 |
| BstNI | CCWGG | 4 cut(s) | 267, 401, 448, 656 |
| BstNSI | RCATGY | 2 cut(s) | 711, 859 |
| BstPI | GGTNACC | 1 cut(s) | 967 |
| BstSCI | CCNGG | 4 cut(s) | 265, 399, 446, 654 |
| BstSFI | CTRYAG | 3 cut(s) | 387, 660, 813 |
| BstV1I | GCAGC | 3 cut(s) | 401, 411, 579 |
| BsuRI | GGCC | 2 cut(s) | 501, 650 |
| BtgI | CCRYGG | 1 cut(s) | 73 |
| BtsCI | GGATG | 2 cut(s) | 93, 607 |
| BtsI | GCAGTG | 1 cut(s) | 384 |
| BtsIMutI | CAGTG | 3 cut(s) | 20, 384, 949 |
| Cac8I | GCNNGC | 2 cut(s) | 17, 42 |
| Cfr13I | GGNCC | 4 cut(s) | 397, 500, 649, 937 |
| CseI | GACGC | 1 cut(s) | 232 |
| Csp6I | GTAC | 3 cut(s) | 299, 436, 818 |
| CviAII | CATG | 8 cut(s) | 74, 81, 222, 380, 515, 681, 708, 856 |
| CviQI | GTAC | 3 cut(s) | 299, 436, 818 |
| DdeI | CTNAG | 3 cut(s) | 124, 248, 941 |
| DpnI | GATC | 5 cut(s) | 220, 530, 842, 862, 1009 |
| DpnII | GATC | 5 cut(s) | 218, 528, 840, 860, 1007 |
| DraI | TTTAAA | 1 cut(s) | 416 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 457 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 457 |
| Eam1104I | CTCTTC | 1 cut(s) | 175 |
| EarI | CTCTTC | 1 cut(s) | 175 |
| Eco130I | CCWWGG | 2 cut(s) | 73, 850 |
| Eco24I | GRGCYC | 1 cut(s) | 692 |
| Eco31I | GGTCTC | 1 cut(s) | 1045 |
| Eco47I | GGWCC | 2 cut(s) | 397, 937 |
| Eco91I | GGTNACC | 1 cut(s) | 967 |
| EcoO65I | GGTNACC | 1 cut(s) | 967 |
| EcoRII | CCWGG | 4 cut(s) | 265, 399, 446, 654 |
| EcoT14I | CCWWGG | 2 cut(s) | 73, 850 |
| EcoT38I | GRGCYC | 1 cut(s) | 692 |
| ErhI | CCWWGG | 2 cut(s) | 73, 850 |
| FaeI | CATG | 8 cut(s) | 77, 84, 225, 383, 518, 684, 711, 859 |
| FatI | CATG | 8 cut(s) | 73, 80, 221, 379, 514, 680, 707, 855 |
| FbaI | TGATCA | 2 cut(s) | 218, 528 |
| Fnu4HI | GCNGC | 3 cut(s) | 390, 425, 593 |
| FokI | GGATG | 2 cut(s) | 80, 594 |
| FriOI | GRGCYC | 1 cut(s) | 692 |
| Fsp4HI | GCNGC | 3 cut(s) | 390, 425, 593 |
| FspBI | CTAG | 2 cut(s) | 542, 772 |
| GluI | GCNGC | 3 cut(s) | 390, 425, 593 |
| GsuI | CTGGAG | 1 cut(s) | 853 |
| HaeIII | GGCC | 2 cut(s) | 501, 650 |
| HgaI | GACGC | 1 cut(s) | 232 |
| Hin1I | GRCGYC | 1 cut(s) | 243 |
| Hin1II | CATG | 8 cut(s) | 77, 84, 225, 383, 518, 684, 711, 859 |
| HinfI | GANTC | 4 cut(s) | 327, 519, 742, 894 |
| HphI | GGTGA | 1 cut(s) | 979 |
| Hpy166II | GTNNAC | 1 cut(s) | 351 |
| Hpy188I | TCNGA | 1 cut(s) | 792 |
| Hpy188III | TCNNGA | 5 cut(s) | 64, 247, 832, 915, 958 |
| Hpy8I | GTNNAC | 1 cut(s) | 351 |
| HpyAV | CCTTC | 4 cut(s) | 108, 364, 385, 892 |
| HpyCH4III | ACNGT | 3 cut(s) | 24, 454, 1069 |
| HpyCH4V | TGCA | 6 cut(s) | 155, 389, 410, 473, 595, 815 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 656 |
| HpyF3I | CTNAG | 3 cut(s) | 124, 248, 941 |
| Hsp92I | GRCGYC | 1 cut(s) | 243 |
| Hsp92II | CATG | 8 cut(s) | 77, 84, 225, 383, 518, 684, 711, 859 |
| Ksp22I | TGATCA | 2 cut(s) | 218, 528 |
| Kzo9I | GATC | 5 cut(s) | 218, 528, 840, 860, 1007 |
| LmnI | GCTCC | 1 cut(s) | 168 |
| Lsp1109I | GCAGC | 3 cut(s) | 401, 411, 579 |
| LweI | GCATC | 1 cut(s) | 616 |
| MaeI | CTAG | 2 cut(s) | 542, 772 |
| MaeIII | GTNAC | 3 cut(s) | 382, 967, 997 |
| MalI | GATC | 5 cut(s) | 220, 530, 842, 862, 1009 |
| MboI | GATC | 5 cut(s) | 218, 528, 840, 860, 1007 |
| MboII | GAAGA | 3 cut(s) | 192, 309, 939 |
| MfeI | CAATTG | 2 cut(s) | 35, 203 |
| MhlI | GDGCHC | 1 cut(s) | 692 |
| MluCI | AATT | 9 cut(s) | 35, 67, 114, 145, 203, 510, 572, 780, 1020 |
| MlyI | GAGTC | 1 cut(s) | 513 |
| MseI | TTAA | 5 cut(s) | 415, 619, 716, 932, 1028 |
| MspR9I | CCNGG | 4 cut(s) | 267, 401, 448, 656 |
| MunI | CAATTG | 2 cut(s) | 35, 203 |
| MvaI | CCWGG | 4 cut(s) | 267, 401, 448, 656 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 656 |
| NcoI | CCATGG | 1 cut(s) | 73 |
| NdeII | GATC | 5 cut(s) | 218, 528, 840, 860, 1007 |
| NlaIII | CATG | 8 cut(s) | 77, 84, 225, 383, 518, 684, 711, 859 |
| NmuCI | GTSAC | 2 cut(s) | 382, 967 |
| NspI | RCATGY | 2 cut(s) | 711, 859 |
| PciI | ACATGT | 1 cut(s) | 707 |
| PfeI | GAWTC | 3 cut(s) | 327, 742, 894 |
| PkrI | GCNGC | 3 cut(s) | 391, 426, 594 |
| PleI | GAGTC | 1 cut(s) | 513 |
| PpsI | GAGTC | 1 cut(s) | 513 |
| PscI | ACATGT | 1 cut(s) | 707 |
| PshBI | ATTAAT | 1 cut(s) | 932 |
| Psp6I | CCWGG | 4 cut(s) | 265, 399, 446, 654 |
| PspEI | GGTNACC | 1 cut(s) | 967 |
| PspGI | CCWGG | 4 cut(s) | 265, 399, 446, 654 |
| PspPI | GGNCC | 4 cut(s) | 397, 500, 649, 937 |
| PstI | CTGCAG | 2 cut(s) | 391, 817 |
| RsaI | GTAC | 3 cut(s) | 300, 437, 819 |
| RsaNI | GTAC | 3 cut(s) | 299, 436, 818 |
| SaqAI | TTAA | 5 cut(s) | 415, 619, 716, 932, 1028 |
| SatI | GCNGC | 3 cut(s) | 390, 425, 593 |
| Sau3AI | GATC | 5 cut(s) | 218, 528, 840, 860, 1007 |
| Sau96I | GGNCC | 4 cut(s) | 397, 500, 649, 937 |
| ScaI | AGTACT | 2 cut(s) | 300, 819 |
| SchI | GAGTC | 1 cut(s) | 513 |
| ScrFI | CCNGG | 4 cut(s) | 267, 401, 448, 656 |
| SduI | GDGCHC | 1 cut(s) | 692 |
| SfaNI | GCATC | 1 cut(s) | 616 |
| SfcI | CTRYAG | 3 cut(s) | 387, 660, 813 |
| SinI | GGWCC | 2 cut(s) | 397, 937 |
| SmlI | CTYRAG | 1 cut(s) | 685 |
| SmoI | CTYRAG | 1 cut(s) | 685 |
| Sse9I | AATT | 9 cut(s) | 35, 67, 114, 145, 203, 510, 572, 780, 1020 |
| SspI | AATATT | 1 cut(s) | 720 |
| SspMI | CTAG | 2 cut(s) | 542, 772 |
| StyD4I | CCNGG | 4 cut(s) | 265, 399, 446, 654 |
| StyI | CCWWGG | 2 cut(s) | 73, 850 |
| TaaI | ACNGT | 3 cut(s) | 24, 454, 1069 |
| TaqI | TCGA | 2 cut(s) | 345, 906 |
| TasI | AATT | 9 cut(s) | 35, 67, 114, 145, 203, 510, 572, 780, 1020 |
| TatI | WGTACW | 2 cut(s) | 298, 817 |
| TfiI | GAWTC | 3 cut(s) | 327, 742, 894 |
| Tru1I | TTAA | 5 cut(s) | 415, 619, 716, 932, 1028 |
| Tru9I | TTAA | 5 cut(s) | 415, 619, 716, 932, 1028 |
| TscAI | CASTG | 3 cut(s) | 27, 391, 949 |
| TseFI | GTSAC | 2 cut(s) | 382, 967 |
| TseI | GCWGC | 3 cut(s) | 389, 424, 592 |
| Tsp45I | GTSAC | 2 cut(s) | 382, 967 |
| TspDTI | ATGAA | 3 cut(s) | 193, 274, 503 |
| TspGWI | ACGGA | 1 cut(s) | 616 |
| TspRI | CASTG | 3 cut(s) | 27, 391, 949 |
| VpaK11BI | GGWCC | 2 cut(s) | 397, 937 |
| VspI | ATTAAT | 1 cut(s) | 932 |
| XapI | RAATTY | 3 cut(s) | 67, 145, 780 |
| XceI | RCATGY | 2 cut(s) | 711, 859 |
| XcmI | CCANNNNNNNNNTGG | 3 cut(s) | 27, 618, 643 |
| XspI | CTAG | 2 cut(s) | 542, 772 |
| ZrmI | AGTACT | 2 cut(s) | 300, 819 |