Rroxscaffold_3G00227460

Glycine-rich domain-containing protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000142
Physical Location & Seq
Forward (+)
11594115 .. 11598561
4447 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00227460.1

Sequence Viewer

Length: 651 bp
ATGGACCCTGTCAATGACAATGCTCAAGATGTTCAAAACAAGTGGGCTAAAAGAATCGCTATGCCCACTTTTTGGAGGTGGACGCATCGGGGCTGGCGGGCACGTAACTTGCTCGGTGGAGGCAGTTTGCATCCTTTGTCTTTCGGGGTTGTTGAAGATGTTCCCTTGTTGGCATCCGTAGCTTCATACACTTATGATTCTAGCTCAAGTTGTTTCATGAACAGATCAAGGAAGAGTGGCTGTTATTGCCTTGGATCAGATCAGCTTTCATACTTTCGGATGTTATGTCCGATGACGGCTATGATGGTTTCAGAAACTACCAGTGGAGAGAAGCAATCAGAATTTATTACTCAACAGATCGACGCTGTTAACATTAAGCGCAATGAGAAAGAAAAAAGAGCCAATTTGTTCACTGCCTCGGAAAAAATGAAACTTGAGGGGGATAATGAGAGTTTAAACGTATGGGAGGCCCTGAAAAAAGGTGGCTCATGTGGCCCTTATGAAGCTGGCTTTAACCCCTACAATATGACAGAAGGTAGCCATAGTGGAATAAGATGTGGCAGCTGTGGCGGTGGTTGTGGCAGTTGTGGCCAGTGCCGGGGTGGTCAGTGTGGGGGAGGCAAATGCAGTTTTAGTTGCGGTAGCAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRDP-like

Protein Analysis

216

Amino Acids

23.56

Weight (kDa)

8.55

Isoelectric Point (pI)

52.83

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000142)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g13532 FvH4_2g28870 FvH4_3g30930 FvH4_3g37281 FvH4_3g37300 FvH4_3g37301 FvH4_3g37301 FvH4_4g23711 FvH4_5g02330 FvH4_6g16960 FvH4_6g16971 FvH4_6g21291 FvH4_6g21331 FvH4_6g22212 FvH4_6g22332 FvH4_6g36920 FvH4_6g37552 FvH4_6g37591 FvH4_6g39721 FvH4_6g46020 FvH4_6g47001 FvH4_6g47002 FvH4_6g47130 FvH4_6g47140 FvH4_6g47161 FvH4_6g47170 FvH4_6g47190 FvH4_6g49120 FvH4_6g49280 FvH4_6g49281 FvH4_6g49640 FvH4_6g49640
malus_domestica MD03G1085600.v1.1 MD09G1027500.v1.1 MD09G1050100.v1.1
prunus_persica Prupe.1G004300_v2.0.a1 Prupe.1G016300_v2.0.a1 Prupe.1G019300_v2.0.a1 Prupe.3G239100_v2.0.a1 Prupe.3G291200_v2.0.a1
pyrus_communis pycom08g02840 pycom08g02860 pycom08g02870
rosa_chinensis RchiOBHm_Chr2g0153831 RchiOBHm_Chr2g0166171 RchiOBHm_Chr2g0166201 RchiOBHm_Chr3g0476221 RchiOBHm_Chr4g0430971 RchiOBHm_Chr5g0067081 RchiOBHm_Chr7g0197821
rosa_laevigata RLG00000001284 RLG00000006996 RLG00000012710 RLG00000019299 RLG00000020285 RLG00000020444 RLG00000020466 RLG00000020591 RLG00000020665 RLG00000020730 RLG00000020732 RLG00000021449 RLG00000021592 RLG00000021593 RLG00000021594 RLG00000021596 RLG00000021597 RLG00000021801 RLG00000023534 RLG00000023538 RLG00000023693 RLG00000023694 RLG00000023699 RLG00000024196 RLG00000024214 RLG00000024220 RLG00000024244 RLG00000024608 RLG00000024612 RLG00000035157 RLG00000035906
rosa_multiflora Rmu_co8282815.1_g000001 Rmu_co8380495.1_g000001 Rmu_co8381111.1_g000001 Rmu_co8460513.1_g000001 Rmu_co8481947.1_g000001 Rmu_co8487937.1_g000001 Rmu_sc0000151.1_g000017 Rmu_sc0001427.1_g000003 Rmu_sc0001427.1_g000006 Rmu_sc0002611.1_g000008 Rmu_sc0003887.1_g000018 Rmu_sc0003887.1_g000019 Rmu_sc0003887.1_g000021 Rmu_sc0004119.1_g000004 Rmu_sc0004329.1_g000003 Rmu_sc0004485.1_g000024 Rmu_sc0005290.1_g000001 Rmu_sc0005609.1_g000006 Rmu_sc0006187.1_g000018 Rmu_sc0006218.1_g000032 Rmu_sc0006218.1_g000033 Rmu_sc0007150.1_g000003 Rmu_sc0007154.1_g000004 Rmu_sc0007154.1_g000005 Rmu_sc0007154.1_g000007 Rmu_sc0008314.1_g000012 Rmu_sc0010073.1_g000023 Rmu_sc0010073.1_g000025 Rmu_sc0011216.1_g000008 Rmu_sc0012445.1_g000005 Rmu_sc0013571.1_g000002 Rmu_sc0018648.1_g000002 Rmu_sc0020961.1_g000002 Rmu_sc0020961.1_g000003 Rmu_sc0023710.1_g000001 Rmu_sc0031310.1_g000008 Rmu_sc0033940.1_g000001 Rmu_sc0035493.1_g000001 Rmu_sc0039285.1_g000001
rosa_roxburghii Rroxscaffold_1G00014000 Rroxscaffold_1G00014020 Rroxscaffold_2G00082760 Rroxscaffold_2G00085090 Rroxscaffold_2G00085100 Rroxscaffold_2G00085140 Rroxscaffold_2G00086670 Rroxscaffold_2G00086680 Rroxscaffold_2G00098190 Rroxscaffold_2G00099270 Rroxscaffold_3G00227460 Rroxscaffold_3G00258190 Rroxscaffold_3G00259780 Rroxscaffold_6G00402940 Rroxscaffold_6G00410200 Rroxscaffold_6G00410210 Rroxscaffold_7G00184320
rosa_rugosa Rorug02G0416100 Rorug02G0416200 Rorug02G0520900 Rorug02G0521200 Rorug02G0521300 Rorug02G0534600 Rorug03G0116300 Rorug03G0162900 Rorug03G0162900 Rorug04G0241400 Rorug05G0380900 Rorug07G0043800
rosa_samantha Rh1AG115300 Rh2BG483700 Rh2BG509900 Rh2BG515700 Rh2BG515800 Rh2BG586500 Rh2BG586600 Rh2BG586700 Rh2BG598800 Rh2BG598900 Rh2BG599100 Rh2BG618400 Rh2BG630500 Rh2CG457400 Rh2CG484900 Rh3AG165300 Rh3AG212900 Rh3AG213300 Rh3BG145800 Rh3BG146100 Rh3BG235300 Rh3BG246500 Rh3BG247000 Rh3BG247100 Rh3CG240300 Rh3CG240800 Rh3CG240900 Rh3CG259600 Rh3DG147000 Rh3DG184800 Rh3DG184900 Rh3DG185000 Rh3DG239500 Rh3DG240100 Rh3DG240200 Rh3DG255800 Rh5BG458500 Rh5CG480400 Rh7BG170400 Rh7BG407900 Rh7CG176900
rosa_wichuraiana Rw2G038990 Rw2G041550 Rw2G047660 Rw2G048940 Rw4G026370 Rw6G008730 Rw7G014600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 72
AciI CCGC 3 cut(s) 97, 570, 639
AclWI GGATC 1 cut(s) 262
AcoI YGGCCR 1 cut(s) 589
AcsI RAATTY 1 cut(s) 341
AfiI CCNNNNNNNGG 2 cut(s) 72, 598
AgsI TTSAA 2 cut(s) 35, 155
AluBI AGCT 5 cut(s) 182, 204, 265, 506, 564
AluI AGCT 5 cut(s) 182, 204, 265, 506, 564
AlwI GGATC 1 cut(s) 262
AoxI GGCC 3 cut(s) 468, 493, 589
ApeKI GCWGC 1 cut(s) 561
ApoI RAATTY 1 cut(s) 341
Asp700I GAANNNNTTC 1 cut(s) 159
AspLEI GCGC 1 cut(s) 381
AspS9I GGNCC 3 cut(s) 4, 469, 494
AsuC2I CCSGG 1 cut(s) 599
AvaII GGWCC 1 cut(s) 4
BaeGI GKGCMC 1 cut(s) 103
BalI TGGCCA 1 cut(s) 591
BbvI GCAGC 1 cut(s) 573
BccI CCATC 1 cut(s) 298
BceAI ACGGC 1 cut(s) 312
BcnI CCSGG 1 cut(s) 599
BfaI CTAG 1 cut(s) 201
BisI GCNGC 1 cut(s) 562
BlsI GCNGC 1 cut(s) 563
Bme1390I CCNGG 1 cut(s) 599
Bme18I GGWCC 1 cut(s) 4
BmgT120I GGNCC 3 cut(s) 4, 469, 494
BmiI GGNNCC 1 cut(s) 6
BmrFI CCNGG 1 cut(s) 599
BmsI GCATC 3 cut(s) 94, 139, 182
BpuEI CTTGAG 3 cut(s) 9, 190, 455
BpuMI CCSGG 1 cut(s) 599
BsaAI YACGTR 1 cut(s) 104
BsaJI CCNNGG 3 cut(s) 250, 417, 598
Bsc4I CCNNNNNNNGG 2 cut(s) 72, 598
Bse1I ACTGG 2 cut(s) 321, 592
Bse3DI GCAATG 1 cut(s) 388
BseDI CCNNGG 3 cut(s) 250, 417, 598
BseGI GGATG 3 cut(s) 130, 173, 285
BseLI CCNNNNNNNGG 2 cut(s) 72, 598
BseMI GCAATG 1 cut(s) 388
BseNI ACTGG 2 cut(s) 321, 592
BseSI GKGCMC 1 cut(s) 103
BseXI GCAGC 1 cut(s) 573
BshFI GGCC 3 cut(s) 470, 495, 591
BsiSI CCGG 1 cut(s) 598
BslI CCNNNNNNNGG 2 cut(s) 72, 598
BsnI GGCC 3 cut(s) 470, 495, 591
Bsp1286I GDGCHC 1 cut(s) 103
Bsp143I GATC 4 cut(s) 224, 254, 259, 357
BspACI CCGC 3 cut(s) 97, 570, 639
BspANI GGCC 3 cut(s) 470, 495, 591
BspHI TCATGA 1 cut(s) 216
BspLI GGNNCC 1 cut(s) 6
BspPI GGATC 1 cut(s) 262
BsrDI GCAATG 1 cut(s) 388
BsrI ACTGG 2 cut(s) 321, 592
BssECI CCNNGG 3 cut(s) 250, 417, 598
BssMI GATC 4 cut(s) 224, 254, 259, 357
BssT1I CCWWGG 1 cut(s) 250
Bst6I CTCTTC 1 cut(s) 227
BstBAI YACGTR 1 cut(s) 104
BstC8I GCNNGC 3 cut(s) 95, 99, 508
BstF5I GGATG 3 cut(s) 130, 173, 285
BstHHI GCGC 1 cut(s) 381
BstKTI GATC 4 cut(s) 227, 257, 262, 360
BstMBI GATC 4 cut(s) 224, 254, 259, 357
BstMWI GCNNNNNNNGC 5 cut(s) 179, 246, 492, 567, 588
BstSCI CCNGG 1 cut(s) 597
BstSLI GKGCMC 1 cut(s) 103
BstV1I GCAGC 1 cut(s) 573
BsuRI GGCC 3 cut(s) 470, 495, 591
BtsCI GGATG 3 cut(s) 130, 173, 285
BtsI GCAGTG 1 cut(s) 411
BtsIMutI CAGTG 4 cut(s) 328, 411, 599, 614
Cac8I GCNNGC 3 cut(s) 95, 99, 508
CciI TCATGA 1 cut(s) 216
CfoI GCGC 1 cut(s) 381
Cfr13I GGNCC 3 cut(s) 4, 469, 494
CseI GACGC 2 cut(s) 91, 371
CspCI CAANNNNNGTGG 2 cut(s) 23, 58
CviAII CATG 2 cut(s) 217, 489
DpnI GATC 4 cut(s) 226, 256, 261, 359
DpnII GATC 4 cut(s) 224, 254, 259, 357
DraI TTTAAA 1 cut(s) 456
EaeI YGGCCR 1 cut(s) 589
Eam1104I CTCTTC 1 cut(s) 227
EarI CTCTTC 1 cut(s) 227
Eco130I CCWWGG 1 cut(s) 250
Eco47I GGWCC 1 cut(s) 4
EcoO109I RGGNCCY 1 cut(s) 469
EcoT14I CCWWGG 1 cut(s) 250
ErhI CCWWGG 1 cut(s) 250
FaeI CATG 2 cut(s) 220, 492
FatI CATG 2 cut(s) 216, 488
FauI CCCGC 1 cut(s) 90
Fnu4HI GCNGC 1 cut(s) 562
FokI GGATG 3 cut(s) 117, 160, 292
Fsp4HI GCNGC 1 cut(s) 562
FspBI CTAG 1 cut(s) 201
GlaI GCGC 1 cut(s) 380
GluI GCNGC 1 cut(s) 562
HaeIII GGCC 3 cut(s) 470, 495, 591
HapII CCGG 1 cut(s) 598
HgaI GACGC 2 cut(s) 91, 371
HhaI GCGC 1 cut(s) 381
Hin1II CATG 2 cut(s) 220, 492
Hin6I GCGC 1 cut(s) 379
HinP1I GCGC 1 cut(s) 379
HincII GTYRAC 1 cut(s) 370
HindII GTYRAC 1 cut(s) 370
HinfI GANTC 2 cut(s) 54, 197
HpaI GTTAAC 1 cut(s) 370
HpaII CCGG 1 cut(s) 598
Hpy166II GTNNAC 3 cut(s) 81, 370, 411
Hpy188I TCNGA 6 cut(s) 259, 279, 291, 313, 340, 421
Hpy188III TCNNGA 2 cut(s) 26, 217
Hpy8I GTNNAC 3 cut(s) 81, 370, 411
Hpy99I CGWCG 1 cut(s) 365
HpyAV CCTTC 1 cut(s) 527
HpyCH4IV ACGT 2 cut(s) 103, 459
HpyCH4V TGCA 2 cut(s) 130, 627
HpyF10VI GCNNNNNNNGC 5 cut(s) 179, 246, 492, 567, 588
HpySE526I ACGT 2 cut(s) 103, 459
Hsp92II CATG 2 cut(s) 220, 492
HspAI GCGC 1 cut(s) 379
KspAI GTTAAC 1 cut(s) 370
Kzo9I GATC 4 cut(s) 224, 254, 259, 357
LpnPI CCDG 7 cut(s) 21, 79, 334, 485, 492, 605, 611
Lsp1109I GCAGC 1 cut(s) 573
LweI GCATC 3 cut(s) 94, 139, 182
MaeI CTAG 1 cut(s) 201
MaeII ACGT 2 cut(s) 103, 459
MaeIII GTNAC 1 cut(s) 104
MalI GATC 4 cut(s) 226, 256, 261, 359
MboI GATC 4 cut(s) 224, 254, 259, 357
MboII GAAGA 2 cut(s) 167, 244
MhlI GDGCHC 1 cut(s) 103
MlsI TGGCCA 1 cut(s) 591
MluCI AATT 2 cut(s) 341, 403
MluNI TGGCCA 1 cut(s) 591
MnlI CCTC 6 cut(s) 69, 113, 427, 430, 460, 611
Mox20I TGGCCA 1 cut(s) 591
MroXI GAANNNNTTC 1 cut(s) 159
MscI TGGCCA 1 cut(s) 591
MseI TTAA 4 cut(s) 369, 375, 455, 513
Msp20I TGGCCA 1 cut(s) 591
MspA1I CMGCKG 1 cut(s) 564
MspI CCGG 1 cut(s) 598
MspR9I CCNGG 1 cut(s) 599
MssI GTTTAAAC 1 cut(s) 456
MwoI GCNNNNNNNGC 5 cut(s) 179, 246, 492, 567, 588
NciI CCSGG 1 cut(s) 599
NdeII GATC 4 cut(s) 224, 254, 259, 357
NlaIII CATG 2 cut(s) 220, 492
NlaIV GGNNCC 1 cut(s) 6
PagI TCATGA 1 cut(s) 216
PdmI GAANNNNTTC 1 cut(s) 159
PfeI GAWTC 2 cut(s) 54, 197
PflFI GACNNNGTC 1 cut(s) 8
PflMI CCANNNNNTGG 1 cut(s) 72
PkrI GCNGC 1 cut(s) 563
PmeI GTTTAAAC 1 cut(s) 456
Ppu21I YACGTR 1 cut(s) 104
PspN4I GGNNCC 1 cut(s) 6
PspPI GGNCC 3 cut(s) 4, 469, 494
PsyI GACNNNGTC 1 cut(s) 8
PvuII CAGCTG 1 cut(s) 564
SaqAI TTAA 4 cut(s) 369, 375, 455, 513
SatI GCNGC 1 cut(s) 562
Sau3AI GATC 4 cut(s) 224, 254, 259, 357
Sau96I GGNCC 3 cut(s) 4, 469, 494
ScrFI CCNGG 1 cut(s) 599
SduI GDGCHC 1 cut(s) 103
SfaNI GCATC 3 cut(s) 94, 139, 182
SinI GGWCC 1 cut(s) 4
SmlI CTYRAG 3 cut(s) 24, 205, 434
SmoI CTYRAG 3 cut(s) 24, 205, 434
Sse9I AATT 2 cut(s) 341, 403
SsiI CCGC 3 cut(s) 97, 570, 639
SspMI CTAG 1 cut(s) 201
StyD4I CCNGG 1 cut(s) 597
StyI CCWWGG 1 cut(s) 250
TaiI ACGT 2 cut(s) 106, 462
TaqI TCGA 1 cut(s) 360
TasI AATT 2 cut(s) 341, 403
TfiI GAWTC 2 cut(s) 54, 197
Tru1I TTAA 4 cut(s) 369, 375, 455, 513
Tru9I TTAA 4 cut(s) 369, 375, 455, 513
TscAI CASTG 4 cut(s) 328, 418, 599, 614
TseI GCWGC 1 cut(s) 561
TspDTI ATGAA 6 cut(s) 174, 205, 233, 258, 443, 516
TspGWI ACGGA 1 cut(s) 166
TspRI CASTG 4 cut(s) 328, 418, 599, 614
Tth111I GACNNNGTC 1 cut(s) 8
Van91I CCANNNNNTGG 1 cut(s) 72
VpaK11BI GGWCC 1 cut(s) 4
XapI RAATTY 1 cut(s) 341
XcmI CCANNNNNNNNNTGG 1 cut(s) 599
XmnI GAANNNNTTC 1 cut(s) 159
XspI CTAG 1 cut(s) 201
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.