Required for maturation of ribosomal RNAs and formation of the large ribosomal subunit
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1413 |
| AarI | CACCTGC | 1 cut(s) | 285 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 836 |
| Acc36I | ACCTGC | 1 cut(s) | 285 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 297 |
| AciI | CCGC | 3 cut(s) | 172, 1498, 1553 |
| AcsI | RAATTY | 4 cut(s) | 144, 205, 524, 1159 |
| AcyI | GRCGYC | 1 cut(s) | 622 |
| AfaI | GTAC | 3 cut(s) | 254, 679, 816 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 240, 297, 716, 1493 |
| AflIII | ACRYGT | 2 cut(s) | 1086, 1540 |
| AgsI | TTSAA | 8 cut(s) | 152, 215, 1016, 1121, 1277, 1286, 1294, 1439 |
| AjnI | CCWGG | 3 cut(s) | 644, 778, 825 |
| AluBI | AGCT | 5 cut(s) | 223, 542, 803, 920, 1038 |
| AluI | AGCT | 5 cut(s) | 223, 542, 803, 920, 1038 |
| Alw21I | GWGCWC | 1 cut(s) | 724 |
| Alw26I | GTCTC | 2 cut(s) | 1371, 1424 |
| Ama87I | CYCGRG | 1 cut(s) | 723 |
| AoxI | GGCC | 2 cut(s) | 878, 1027 |
| ApeKI | GCWGC | 3 cut(s) | 768, 803, 971 |
| ApoI | RAATTY | 4 cut(s) | 144, 205, 524, 1159 |
| AseI | ATTAAT | 1 cut(s) | 1311 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 9 |
| AspLEI | GCGC | 1 cut(s) | 128 |
| AspS9I | GGNCC | 4 cut(s) | 300, 879, 1028, 1316 |
| AsuHPI | GGTGA | 2 cut(s) | 1262, 1358 |
| AsuII | TTCGAA | 2 cut(s) | 25, 1137 |
| AvaI | CYCGRG | 1 cut(s) | 723 |
| AvaII | GGWCC | 2 cut(s) | 300, 1316 |
| AxyI | CCTNAGG | 2 cut(s) | 105, 627 |
| BanII | GRGCYC | 1 cut(s) | 1071 |
| Bbv12I | GWGCWC | 1 cut(s) | 724 |
| BbvI | GCAGC | 3 cut(s) | 780, 790, 958 |
| BccI | CCATC | 4 cut(s) | 745, 950, 1291, 1495 |
| BciT130I | CCWGG | 3 cut(s) | 646, 780, 827 |
| BclI | TGATCA | 2 cut(s) | 597, 907 |
| BcoDI | GTCTC | 2 cut(s) | 1371, 1424 |
| BfaI | CTAG | 3 cut(s) | 921, 1035, 1151 |
| BfmI | CTRYAG | 3 cut(s) | 766, 1039, 1192 |
| BfuAI | ACCTGC | 1 cut(s) | 285 |
| BisI | GCNGC | 3 cut(s) | 769, 804, 972 |
| BlsI | GCNGC | 3 cut(s) | 770, 805, 973 |
| BmcAI | AGTACT | 1 cut(s) | 679 |
| Bme1390I | CCNGG | 3 cut(s) | 646, 780, 827 |
| Bme18I | GGWCC | 2 cut(s) | 300, 1316 |
| BmeT110I | CYCGRG | 1 cut(s) | 723 |
| BmgT120I | GGNCC | 4 cut(s) | 300, 879, 1028, 1316 |
| BmrFI | CCNGG | 3 cut(s) | 646, 780, 827 |
| BmrI | ACTGGG | 1 cut(s) | 1065 |
| BmsI | GCATC | 4 cut(s) | 720, 995, 1458, 1542 |
| BmuI | ACTGGG | 1 cut(s) | 1065 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 717, 749 |
| BpmI | CTGGAG | 1 cut(s) | 56 |
| Bpu14I | TTCGAA | 2 cut(s) | 25, 1137 |
| BpuEI | CTTGAG | 2 cut(s) | 1085, 1232 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1120 |
| BsaHI | GRCGYC | 1 cut(s) | 622 |
| BsaI | GGTCTC | 1 cut(s) | 1424 |
| BsaJI | CCNNGG | 5 cut(s) | 452, 644, 1229, 1342, 1610 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 240, 297, 716, 1493 |
| Bse1I | ACTGG | 2 cut(s) | 1071, 1076 |
| Bse21I | CCTNAGG | 2 cut(s) | 105, 627 |
| Bse3DI | GCAATG | 2 cut(s) | 778, 1030 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1120 |
| BseBI | CCWGG | 3 cut(s) | 646, 780, 827 |
| BseDI | CCNNGG | 5 cut(s) | 452, 644, 1229, 1342, 1610 |
| BseGI | GGATG | 3 cut(s) | 13, 472, 986 |
| BseJI | GATNNNNATC | 1 cut(s) | 1120 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 240, 297, 716, 1493 |
| BseMI | GCAATG | 2 cut(s) | 778, 1030 |
| BseMII | CTCAG | 5 cut(s) | 96, 151, 618, 1334, 1435 |
| BseNI | ACTGG | 2 cut(s) | 1071, 1076 |
| BseRI | GAGGAG | 2 cut(s) | 122, 1187 |
| BseXI | GCAGC | 3 cut(s) | 780, 790, 958 |
| BsgI | GTGCAG | 1 cut(s) | 396 |
| BshFI | GGCC | 2 cut(s) | 880, 1029 |
| BsiHKAI | GWGCWC | 1 cut(s) | 724 |
| BsiHKCI | CYCGRG | 1 cut(s) | 723 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 240, 297, 716, 1493 |
| BsmAI | GTCTC | 2 cut(s) | 1371, 1424 |
| BsmI | GAATGC | 1 cut(s) | 281 |
| BsnI | GGCC | 2 cut(s) | 880, 1029 |
| Bso31I | GGTCTC | 1 cut(s) | 1424 |
| BsoBI | CYCGRG | 1 cut(s) | 723 |
| Bsp119I | TTCGAA | 2 cut(s) | 25, 1137 |
| Bsp1286I | GDGCHC | 2 cut(s) | 724, 1071 |
| Bsp143I | GATC | 2 cut(s) | 597, 907 |
| BspACI | CCGC | 3 cut(s) | 172, 1498, 1553 |
| BspANI | GGCC | 2 cut(s) | 880, 1029 |
| BspCNI | CTCAG | 5 cut(s) | 97, 150, 619, 1333, 1434 |
| BspMAI | CTGCAG | 2 cut(s) | 770, 1196 |
| BspMI | ACCTGC | 1 cut(s) | 285 |
| BspT104I | TTCGAA | 2 cut(s) | 25, 1137 |
| BspTNI | GGTCTC | 1 cut(s) | 1424 |
| BsrDI | GCAATG | 2 cut(s) | 778, 1030 |
| BsrI | ACTGG | 2 cut(s) | 1071, 1076 |
| BssECI | CCNNGG | 5 cut(s) | 452, 644, 1229, 1342, 1610 |
| BssMI | GATC | 2 cut(s) | 597, 907 |
| BssNI | GRCGYC | 1 cut(s) | 622 |
| BssT1I | CCWWGG | 2 cut(s) | 1229, 1610 |
| Bst2UI | CCWGG | 3 cut(s) | 646, 780, 827 |
| Bst4CI | ACNGT | 2 cut(s) | 345, 833 |
| Bst6I | CTCTTC | 1 cut(s) | 554 |
| BstACI | GRCGYC | 1 cut(s) | 622 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 54 |
| BstBI | TTCGAA | 2 cut(s) | 25, 1137 |
| BstC8I | GCNNGC | 2 cut(s) | 221, 396 |
| BstDEI | CTNAG | 6 cut(s) | 105, 137, 503, 627, 1320, 1421 |
| BstDSI | CCRYGG | 1 cut(s) | 452 |
| BstF5I | GGATG | 3 cut(s) | 13, 472, 986 |
| BstHHI | GCGC | 1 cut(s) | 128 |
| BstKTI | GATC | 2 cut(s) | 600, 910 |
| BstMAI | GTCTC | 2 cut(s) | 1371, 1424 |
| BstMBI | GATC | 2 cut(s) | 597, 907 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 54, 1035, 1060 |
| BstNI | CCWGG | 3 cut(s) | 646, 780, 827 |
| BstNSI | RCATGY | 3 cut(s) | 126, 1090, 1238 |
| BstSCI | CCNGG | 3 cut(s) | 644, 778, 825 |
| BstSFI | CTRYAG | 3 cut(s) | 766, 1039, 1192 |
| BstV1I | GCAGC | 3 cut(s) | 780, 790, 958 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1611 |
| Bsu36I | CCTNAGG | 2 cut(s) | 105, 627 |
| BsuRI | GGCC | 2 cut(s) | 880, 1029 |
| BtgI | CCRYGG | 1 cut(s) | 452 |
| BtsCI | GGATG | 3 cut(s) | 13, 472, 986 |
| BtsI | GCAGTG | 1 cut(s) | 763 |
| BtsIMutI | CAGTG | 3 cut(s) | 763, 1328, 1429 |
| BveI | ACCTGC | 1 cut(s) | 285 |
| Cac8I | GCNNGC | 2 cut(s) | 221, 396 |
| CfoI | GCGC | 1 cut(s) | 128 |
| Cfr13I | GGNCC | 4 cut(s) | 300, 879, 1028, 1316 |
| CseI | GACGC | 1 cut(s) | 611 |
| Csp6I | GTAC | 3 cut(s) | 253, 678, 815 |
| CviAII | CATG | 9 cut(s) | 123, 325, 390, 601, 759, 894, 1060, 1087, 1235 |
| CviQI | GTAC | 3 cut(s) | 253, 678, 815 |
| DdeI | CTNAG | 6 cut(s) | 105, 137, 503, 627, 1320, 1421 |
| DpnI | GATC | 2 cut(s) | 599, 909 |
| DpnII | GATC | 2 cut(s) | 597, 907 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 836 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 836 |
| Eam1104I | CTCTTC | 1 cut(s) | 554 |
| EarI | CTCTTC | 1 cut(s) | 554 |
| EciI | GGCGGA | 1 cut(s) | 1513 |
| Eco130I | CCWWGG | 2 cut(s) | 1229, 1610 |
| Eco24I | GRGCYC | 1 cut(s) | 1071 |
| Eco31I | GGTCTC | 1 cut(s) | 1424 |
| Eco32I | GATATC | 1 cut(s) | 322 |
| Eco47I | GGWCC | 2 cut(s) | 300, 1316 |
| Eco81I | CCTNAGG | 2 cut(s) | 105, 627 |
| Eco88I | CYCGRG | 1 cut(s) | 723 |
| EcoRII | CCWGG | 3 cut(s) | 644, 778, 825 |
| EcoRV | GATATC | 1 cut(s) | 322 |
| EcoT14I | CCWWGG | 2 cut(s) | 1229, 1610 |
| EcoT38I | GRGCYC | 1 cut(s) | 1071 |
| ErhI | CCWWGG | 2 cut(s) | 1229, 1610 |
| FaeI | CATG | 9 cut(s) | 126, 328, 393, 604, 762, 897, 1063, 1090, 1238 |
| FatI | CATG | 9 cut(s) | 122, 324, 389, 600, 758, 893, 1059, 1086, 1234 |
| FbaI | TGATCA | 2 cut(s) | 597, 907 |
| Fnu4HI | GCNGC | 3 cut(s) | 769, 804, 972 |
| FokI | GGATG | 2 cut(s) | 459, 973 |
| FriOI | GRGCYC | 1 cut(s) | 1071 |
| Fsp4HI | GCNGC | 3 cut(s) | 769, 804, 972 |
| FspBI | CTAG | 3 cut(s) | 921, 1035, 1151 |
| GlaI | GCGC | 1 cut(s) | 127 |
| GluI | GCNGC | 3 cut(s) | 769, 804, 972 |
| GsuI | CTGGAG | 1 cut(s) | 56 |
| HaeIII | GGCC | 2 cut(s) | 880, 1029 |
| HgaI | GACGC | 1 cut(s) | 611 |
| HhaI | GCGC | 1 cut(s) | 128 |
| Hin1I | GRCGYC | 1 cut(s) | 622 |
| Hin1II | CATG | 9 cut(s) | 126, 328, 393, 604, 762, 897, 1063, 1090, 1238 |
| Hin6I | GCGC | 1 cut(s) | 126 |
| HinP1I | GCGC | 1 cut(s) | 126 |
| HinfI | GANTC | 5 cut(s) | 100, 898, 1121, 1273, 1333 |
| HphI | GGTGA | 2 cut(s) | 1262, 1358 |
| Hpy166II | GTNNAC | 3 cut(s) | 255, 1265, 1599 |
| Hpy188I | TCNGA | 6 cut(s) | 140, 274, 358, 672, 1171, 1492 |
| Hpy8I | GTNNAC | 3 cut(s) | 255, 1265, 1599 |
| HpyAV | CCTTC | 4 cut(s) | 234, 487, 764, 1487 |
| HpyCH4III | ACNGT | 2 cut(s) | 345, 833 |
| HpyCH4IV | ACGT | 2 cut(s) | 188, 1540 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 54, 1035, 1060 |
| HpyF3I | CTNAG | 6 cut(s) | 105, 137, 503, 627, 1320, 1421 |
| HpySE526I | ACGT | 2 cut(s) | 188, 1540 |
| Hsp92I | GRCGYC | 1 cut(s) | 622 |
| Hsp92II | CATG | 9 cut(s) | 126, 328, 393, 604, 762, 897, 1063, 1090, 1238 |
| HspAI | GCGC | 1 cut(s) | 126 |
| Ksp22I | TGATCA | 2 cut(s) | 597, 907 |
| Kzo9I | GATC | 2 cut(s) | 597, 907 |
| LmnI | GCTCC | 4 cut(s) | 37, 547, 738, 1530 |
| Lsp1109I | GCAGC | 3 cut(s) | 780, 790, 958 |
| LweI | GCATC | 4 cut(s) | 720, 995, 1458, 1542 |
| MaeI | CTAG | 3 cut(s) | 921, 1035, 1151 |
| MaeII | ACGT | 2 cut(s) | 188, 1540 |
| MaeIII | GTNAC | 5 cut(s) | 75, 761, 1346, 1376, 1543 |
| MalI | GATC | 2 cut(s) | 599, 909 |
| MboI | GATC | 2 cut(s) | 597, 907 |
| MboII | GAAGA | 8 cut(s) | 127, 153, 293, 296, 329, 571, 691, 1318 |
| MfeI | CAATTG | 2 cut(s) | 1399, 1574 |
| MhlI | GDGCHC | 2 cut(s) | 724, 1071 |
| MlyI | GAGTC | 1 cut(s) | 892 |
| MroXI | GAANNNNTTC | 1 cut(s) | 9 |
| MseI | TTAA | 5 cut(s) | 704, 998, 1311, 1407, 1478 |
| MspR9I | CCNGG | 3 cut(s) | 646, 780, 827 |
| MunI | CAATTG | 2 cut(s) | 1399, 1574 |
| Mva1269I | GAATGC | 1 cut(s) | 281 |
| MvaI | CCWGG | 3 cut(s) | 646, 780, 827 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 54, 1035, 1060 |
| NdeII | GATC | 2 cut(s) | 597, 907 |
| NlaIII | CATG | 9 cut(s) | 126, 328, 393, 604, 762, 897, 1063, 1090, 1238 |
| NmuCI | GTSAC | 3 cut(s) | 761, 1346, 1543 |
| NspI | RCATGY | 3 cut(s) | 126, 1090, 1238 |
| NspV | TTCGAA | 2 cut(s) | 25, 1137 |
| PaeR7I | CTCGAG | 1 cut(s) | 723 |
| PaqCI | CACCTGC | 1 cut(s) | 285 |
| PciI | ACATGT | 1 cut(s) | 1086 |
| PctI | GAATGC | 1 cut(s) | 281 |
| PdmI | GAANNNNTTC | 1 cut(s) | 9 |
| PfeI | GAWTC | 4 cut(s) | 100, 1121, 1273, 1333 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 297 |
| PkrI | GCNGC | 3 cut(s) | 770, 805, 973 |
| PleI | GAGTC | 1 cut(s) | 892 |
| PpsI | GAGTC | 1 cut(s) | 892 |
| PscI | ACATGT | 1 cut(s) | 1086 |
| PshBI | ATTAAT | 1 cut(s) | 1311 |
| PsiI | TTATAA | 1 cut(s) | 1413 |
| Psp6I | CCWGG | 3 cut(s) | 644, 778, 825 |
| PspGI | CCWGG | 3 cut(s) | 644, 778, 825 |
| PspPI | GGNCC | 4 cut(s) | 300, 879, 1028, 1316 |
| PspXI | VCTCGAGB | 1 cut(s) | 723 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 996, 1028 |
| PstI | CTGCAG | 2 cut(s) | 770, 1196 |
| RsaI | GTAC | 3 cut(s) | 254, 679, 816 |
| RsaNI | GTAC | 3 cut(s) | 253, 678, 815 |
| SaqAI | TTAA | 5 cut(s) | 704, 998, 1311, 1407, 1478 |
| SatI | GCNGC | 3 cut(s) | 769, 804, 972 |
| Sau3AI | GATC | 2 cut(s) | 597, 907 |
| Sau96I | GGNCC | 4 cut(s) | 300, 879, 1028, 1316 |
| ScaI | AGTACT | 1 cut(s) | 679 |
| SchI | GAGTC | 1 cut(s) | 892 |
| ScrFI | CCNGG | 3 cut(s) | 646, 780, 827 |
| SduI | GDGCHC | 2 cut(s) | 724, 1071 |
| SfaNI | GCATC | 4 cut(s) | 720, 995, 1458, 1542 |
| SfcI | CTRYAG | 3 cut(s) | 766, 1039, 1192 |
| Sfr274I | CTCGAG | 1 cut(s) | 723 |
| SfuI | TTCGAA | 2 cut(s) | 25, 1137 |
| SinI | GGWCC | 2 cut(s) | 300, 1316 |
| SlaI | CTCGAG | 1 cut(s) | 723 |
| SmlI | CTYRAG | 3 cut(s) | 723, 1064, 1211 |
| SmoI | CTYRAG | 3 cut(s) | 723, 1064, 1211 |
| SsiI | CCGC | 3 cut(s) | 172, 1498, 1553 |
| SspMI | CTAG | 3 cut(s) | 921, 1035, 1151 |
| StyD4I | CCNGG | 3 cut(s) | 644, 778, 825 |
| StyI | CCWWGG | 2 cut(s) | 1229, 1610 |
| TaaI | ACNGT | 2 cut(s) | 345, 833 |
| TaiI | ACGT | 2 cut(s) | 191, 1543 |
| TaqI | TCGA | 4 cut(s) | 25, 724, 1137, 1585 |
| TatI | WGTACW | 2 cut(s) | 252, 677 |
| TfiI | GAWTC | 4 cut(s) | 100, 1121, 1273, 1333 |
| Tru1I | TTAA | 5 cut(s) | 704, 998, 1311, 1407, 1478 |
| Tru9I | TTAA | 5 cut(s) | 704, 998, 1311, 1407, 1478 |
| TscAI | CASTG | 3 cut(s) | 770, 1328, 1429 |
| TseFI | GTSAC | 3 cut(s) | 761, 1346, 1543 |
| TseI | GCWGC | 3 cut(s) | 768, 803, 971 |
| Tsp45I | GTSAC | 3 cut(s) | 761, 1346, 1543 |
| TspDTI | ATGAA | 5 cut(s) | 321, 378, 572, 653, 789 |
| TspGWI | ACGGA | 3 cut(s) | 6, 441, 995 |
| TspRI | CASTG | 3 cut(s) | 770, 1328, 1429 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 297 |
| VpaK11BI | GGWCC | 2 cut(s) | 300, 1316 |
| VspI | ATTAAT | 1 cut(s) | 1311 |
| XapI | RAATTY | 4 cut(s) | 144, 205, 524, 1159 |
| XceI | RCATGY | 3 cut(s) | 126, 1090, 1238 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 406, 997 |
| XhoI | CTCGAG | 1 cut(s) | 723 |
| XmnI | GAANNNNTTC | 1 cut(s) | 9 |
| XspI | CTAG | 3 cut(s) | 921, 1035, 1151 |
| ZrmI | AGTACT | 1 cut(s) | 679 |