Rorug02G0416200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000142
Physical Location & Seq
Reverse (-)
53385180 .. 53389486
4307 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0416200.1

Sequence Viewer

Length: 2559 bp
ATGTGTCTGTCTGTCCAAGGCTTCATTAGCATAGACTGTGGCCTACCAGAAAATTCAAGCTATACTGAAAAAGCAACCGGCATCTATTACATTTCAGATGCGATATTCACAGACACCGGTGAAAGTAAATTGGTATTGCATGACAACAGAGATAGCTATCAACATCCTTACTTGTCTTTTAGGAGTTTTCCCAATGGAACCAGGAACTGCTACAAAATAAGCGTCGCAAACGGCACCAAATATTTGATCAGAGCCAGTTTCTACTATGGGAATTATGACGGTCAAAATGAATTGCCAGAATTCGACCTACATCTTGGGCCTAATTACTGGGATACAGTGAGGTTGAAGGATTCAGACACTACAACACACAAGGAGCTCATACATTACACACTTCGGAACTATATACATGTTTGTCTGGTGAAGTCAAACAAAGGAATTCCATTTATATCAGCAATAGAGCTAAGGCCTTTACCTAATGTGATTTATGAAGTAAAGGGCTACTCCTTGGCACTAATCTGGCGATATGACACTGGTCAAACTGCTAAAGAGTACAGGTATCCAAGTGATCTTCACGATCGTCTATGGTATCCCTCTGACCGCGATGATTGGACGCAATTAAATACCTCATTATGCAACACTACCGAGGACAATAGTTACCAAGTTCCATCTATTGTCATGTGTACTGCTGCCACACCCAAAAATGCAGATGATTCCTTGAATATTTTCTGGCTACCTTCTGATAGCAATGCACAGTATCAAATTTACATGCACTTTGCAGAAGTAGAAAAGCTCCAAGCGAACAAGTCTAGACAATTCAGTATTACTTTTAATGGAAAGCTCTTTTATGGGCCCTTTTCTCCTCAATACTTGTCCTTCGATACTATTTATAGCATAGCGGCTTGGAGTCCAACAGGACAATATATAAAGTTTTCCATCTTGAAGAATGAGAAATCTACCCTTCCACCTATCCTCAATGCTTATGAAATATATATGGTCAAGCCAGCTCCCCAATCAGAGACAAAACACGACGACATTGACGCGATCACAAACATCAAGTCAACTTATAAAATTACAAGAAACTGGCAAGGAGATCCATGTGCCCCCCAAAACTACTCTTGGGAAGGCTTGAAGTGTAGCTTTGGGGGGCCTGGAAACTTCCCAAGAATAATATCCTTGGACTTGTCCTCAAGTGGAATAACAGGGGAGATATCTCTTTCTATATCCAATCTCACAATGATAAAGACTTTAGATTTATCAAACAACAACTTGAGAGGATCAATTCCAGAGTTTTTGTCTCAGTTACCAGAATTAGAAGTTTTAAATTTGGAGAAGAACAACTTCACTGGTTTAGTTCCATCAAGACTCATTGAGAGAAGGAACAGCGGCTCACTACTGTTAAGTTTGTGTGAAAATCCATATCTATCCATACAAGTTTCTTGCAAGAAGAAGAAGAAGAAGCACAATGTTGTCCTAGTCGTAGTGCCAATTCTTGTAATTGTCATCCTTTTATTGACTATAGGAGCTGTCCTATTGGGCCTTAAAGTGAAAAGAAAACCTGAGATCCAGAATTCTATTCAGAGCTGGTCATTGGAGTCAGAGGATCGACGCTTTACATACTCAGAGCTTGTGAAGATAACTAATAATTTTGCATCAATCATCGGAAGAGGCGGATTCGGAAGAGTCTACCATGGCACTCTGAAAAATGATATTCAAGTCGCTGTGAAGTTACTGACTTCTTCTTCAAGGCAAGGCTCAAAAGAATTCCAAAATGAGGTTAAACTATTGATGACAGCTCATCATCGAAACTTGGTTTCTCTCATTGGTTACTGCGACGAAGGTGACACTATGGCACTTGTTTTTGAGTATGTAGCCAATGGAAATTTGCAGCAACATTTATCAGATCTAACATTGGACGTTTTGACTTGGAAGGAAAGACTTCAAATTGCCGTGGATGCAGCACGAGGGTTGGATTATCTACATAATGGTTGCAAGCCACCTATAGTCCATAGAGATCTGAAGGCTTCCAATATCTTGTTAAATGAAAAGTTGCAAGCCAAGATAGCTGATTTTGGTCTTTCTAAAGTTCTTGCAACTGAAAGCGCCACTCATATATCAACTGATGCCAAAGGAACATTCGGGTACCTTGATGCTGAATATTGCAATACAGGACAGCTCAACAAAAAGAGTGATGTATACAGCTTTGGGATTGTGTTGCTAGAGCTAATAACCGGTAGAGCACCCATAATAAGAGATGTAGAACCTTTTCCTATTCACATATGTCAATGGGTGACTCCTATATATGAGAACATGGAAATTGAAAAGATAGTTGATTCAAAAATACAGGGTACCTACAACAATTTTTCTGCTCAGAAAGCTATAGAAATCGCCATGGCATGTGTGCGTTCATCAGCAATCCAAAGGCCAGAAATAAGCTCTGTATATGTTGAATTGAAGGGATGTTTGGAAATTGAGATGACTTCTGATCAAGTAACAACAGTTACAGAGATCGATGAGAGCGATGATGATACAAATTCGAGCAGTTCAGTTCTTGTCACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

852

Amino Acids

95.95

Weight (kDa)

5.97

Isoelectric Point (pI)

36.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Malectin_like PF12819 11 - 332 2.3e-112 Malectin-like domain
LRR_8 PF13855 391 - 447 1e-05 Leucine rich repeat
LRR_4 PF12799 413 - 446 1e-05 Leucine Rich repeats (2 copies)
PK_Tyr_Ser-Thr PF07714 550 - 816 4.5e-52 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 551 - 812 1.1e-43 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000142)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g13532 FvH4_2g28870 FvH4_3g30930 FvH4_3g37281 FvH4_3g37300 FvH4_3g37301 FvH4_3g37301 FvH4_4g23711 FvH4_5g02330 FvH4_6g16960 FvH4_6g16971 FvH4_6g21291 FvH4_6g21331 FvH4_6g22212 FvH4_6g22332 FvH4_6g36920 FvH4_6g37552 FvH4_6g37591 FvH4_6g39721 FvH4_6g46020 FvH4_6g47001 FvH4_6g47002 FvH4_6g47130 FvH4_6g47140 FvH4_6g47161 FvH4_6g47170 FvH4_6g47190 FvH4_6g49120 FvH4_6g49280 FvH4_6g49281 FvH4_6g49640 FvH4_6g49640
malus_domestica MD03G1085600.v1.1 MD09G1027500.v1.1 MD09G1050100.v1.1
prunus_persica Prupe.1G004300_v2.0.a1 Prupe.1G016300_v2.0.a1 Prupe.1G019300_v2.0.a1 Prupe.3G239100_v2.0.a1 Prupe.3G291200_v2.0.a1
pyrus_communis pycom08g02840 pycom08g02860 pycom08g02870
rosa_chinensis RchiOBHm_Chr2g0153831 RchiOBHm_Chr2g0166171 RchiOBHm_Chr2g0166201 RchiOBHm_Chr3g0476221 RchiOBHm_Chr4g0430971 RchiOBHm_Chr5g0067081 RchiOBHm_Chr7g0197821
rosa_laevigata RLG00000001284 RLG00000006996 RLG00000012710 RLG00000019299 RLG00000020285 RLG00000020444 RLG00000020466 RLG00000020591 RLG00000020665 RLG00000020730 RLG00000020732 RLG00000021449 RLG00000021592 RLG00000021593 RLG00000021594 RLG00000021596 RLG00000021597 RLG00000021801 RLG00000023534 RLG00000023538 RLG00000023693 RLG00000023694 RLG00000023699 RLG00000024196 RLG00000024214 RLG00000024220 RLG00000024244 RLG00000024608 RLG00000024612 RLG00000035157 RLG00000035906
rosa_multiflora Rmu_co8282815.1_g000001 Rmu_co8380495.1_g000001 Rmu_co8381111.1_g000001 Rmu_co8460513.1_g000001 Rmu_co8481947.1_g000001 Rmu_co8487937.1_g000001 Rmu_sc0000151.1_g000017 Rmu_sc0001427.1_g000003 Rmu_sc0001427.1_g000006 Rmu_sc0002611.1_g000008 Rmu_sc0003887.1_g000018 Rmu_sc0003887.1_g000019 Rmu_sc0003887.1_g000021 Rmu_sc0004119.1_g000004 Rmu_sc0004329.1_g000003 Rmu_sc0004485.1_g000024 Rmu_sc0005290.1_g000001 Rmu_sc0005609.1_g000006 Rmu_sc0006187.1_g000018 Rmu_sc0006218.1_g000032 Rmu_sc0006218.1_g000033 Rmu_sc0007150.1_g000003 Rmu_sc0007154.1_g000004 Rmu_sc0007154.1_g000005 Rmu_sc0007154.1_g000007 Rmu_sc0008314.1_g000012 Rmu_sc0010073.1_g000023 Rmu_sc0010073.1_g000025 Rmu_sc0011216.1_g000008 Rmu_sc0012445.1_g000005 Rmu_sc0013571.1_g000002 Rmu_sc0018648.1_g000002 Rmu_sc0020961.1_g000002 Rmu_sc0020961.1_g000003 Rmu_sc0023710.1_g000001 Rmu_sc0031310.1_g000008 Rmu_sc0033940.1_g000001 Rmu_sc0035493.1_g000001 Rmu_sc0039285.1_g000001
rosa_roxburghii Rroxscaffold_1G00014000 Rroxscaffold_1G00014020 Rroxscaffold_2G00082760 Rroxscaffold_2G00085090 Rroxscaffold_2G00085100 Rroxscaffold_2G00085140 Rroxscaffold_2G00086670 Rroxscaffold_2G00086680 Rroxscaffold_2G00098190 Rroxscaffold_2G00099270 Rroxscaffold_3G00227460 Rroxscaffold_3G00258190 Rroxscaffold_3G00259780 Rroxscaffold_6G00402940 Rroxscaffold_6G00410200 Rroxscaffold_6G00410210 Rroxscaffold_7G00184320
rosa_rugosa Rorug02G0416100 Rorug02G0416200 Rorug02G0520900 Rorug02G0521200 Rorug02G0521300 Rorug02G0534600 Rorug03G0116300 Rorug03G0162900 Rorug03G0162900 Rorug04G0241400 Rorug05G0380900 Rorug07G0043800
rosa_samantha Rh1AG115300 Rh2BG483700 Rh2BG509900 Rh2BG515700 Rh2BG515800 Rh2BG586500 Rh2BG586600 Rh2BG586700 Rh2BG598800 Rh2BG598900 Rh2BG599100 Rh2BG618400 Rh2BG630500 Rh2CG457400 Rh2CG484900 Rh3AG165300 Rh3AG212900 Rh3AG213300 Rh3BG145800 Rh3BG146100 Rh3BG235300 Rh3BG246500 Rh3BG247000 Rh3BG247100 Rh3CG240300 Rh3CG240800 Rh3CG240900 Rh3CG259600 Rh3DG147000 Rh3DG184800 Rh3DG184900 Rh3DG185000 Rh3DG239500 Rh3DG240100 Rh3DG240200 Rh3DG255800 Rh5BG458500 Rh5CG480400 Rh7BG170400 Rh7BG407900 Rh7CG176900
rosa_wichuraiana Rw2G038990 Rw2G041550 Rw2G047660 Rw2G048940 Rw4G026370 Rw6G008730 Rw7G014600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1065
Acc65I GGTACC 2 cut(s) 2139, 2345
AccB1I GGYRCC 3 cut(s) 233, 2139, 2345
AccI GTMKAC 2 cut(s) 1683, 2193
AccII CGCG 2 cut(s) 600, 1040
AciI CCGC 4 cut(s) 598, 896, 1383, 1668
AclWI GGATC 4 cut(s) 1085, 1282, 1555, 1608
AcsI RAATTY 9 cut(s) 52, 299, 435, 759, 1321, 1567, 1760, 1879, 2530
AcuI CTGAAG 1 cut(s) 2036
AfaI GTAC 4 cut(s) 551, 682, 2141, 2347
AfiI CCNNNNNNNGG 1 cut(s) 1771
AflIII ACRYGT 1 cut(s) 406
AgeI ACCGGT 2 cut(s) 116, 2228
AjnI CCWGG 2 cut(s) 200, 1147
AjuI GAANNNNNNNTTGG 4 cut(s) 2117, 2149, 2444, 2476
Alw21I GWGCWC 2 cut(s) 378, 2239
Alw26I GTCTC 2 cut(s) 1010, 1299
AlwI GGATC 4 cut(s) 1085, 1282, 1555, 1608
AlwNI CAGNNNCTG 1 cut(s) 207
AoxI GGCC 7 cut(s) 40, 317, 464, 848, 1145, 1534, 2420
ApaI GGGCCC 1 cut(s) 852
ApeKI GCWGC 3 cut(s) 686, 1885, 1955
ApoI RAATTY 9 cut(s) 52, 299, 435, 759, 1321, 1567, 1760, 1879, 2530
ArsI GACNNNNNNTTYG 4 cut(s) 207, 239, 1040, 1072
AsiGI ACCGGT 2 cut(s) 116, 2228
Asp700I GAANNNNTTC 4 cut(s) 722, 1337, 1935, 2262
Asp718I GGTACC 2 cut(s) 2139, 2345
AspLEI GCGC 1 cut(s) 2102
AspS9I GGNCC 5 cut(s) 317, 848, 849, 1145, 1534
AsuHPI GGTGA 5 cut(s) 131, 430, 1850, 2299, 2545
BaeGI GKGCMC 2 cut(s) 852, 1102
BanI GGYRCC 3 cut(s) 233, 2139, 2345
BanII GRGCYC 2 cut(s) 378, 852
BauI CACGAG 1 cut(s) 1959
Bbv12I GWGCWC 2 cut(s) 378, 2239
BbvI GCAGC 3 cut(s) 673, 1897, 1967
BccI CCATC 3 cut(s) 673, 941, 1363
BceAI ACGGC 2 cut(s) 247, 1931
BciT130I CCWGG 2 cut(s) 202, 1149
BciVI GTATCC 3 cut(s) 325, 567, 597
BclI TGATCA 2 cut(s) 246, 2482
BcoDI GTCTC 2 cut(s) 1010, 1299
BfaI CTAG 3 cut(s) 807, 1472, 2216
BfmI CTRYAG 3 cut(s) 1515, 1998, 2376
BfoI RGCGCY 1 cut(s) 2103
BfuI GTATCC 3 cut(s) 325, 567, 597
BglII AGATCT 2 cut(s) 1900, 2011
BisI GCNGC 5 cut(s) 687, 897, 1384, 1886, 1956
BlsI GCNGC 5 cut(s) 688, 898, 1385, 1887, 1957
Bme1390I CCNGG 2 cut(s) 202, 1149
BmgT120I GGNCC 5 cut(s) 317, 848, 849, 1145, 1534
BmiI GGNNCC 6 cut(s) 199, 235, 850, 1146, 2141, 2347
BmrFI CCNGG 2 cut(s) 202, 1149
BmrI ACTGGG 1 cut(s) 337
BmsI GCATC 6 cut(s) 88, 90, 1658, 1942, 2110, 2137
BmuI ACTGGG 1 cut(s) 337
BoxI GACNNNNGTC 1 cut(s) 531
Bpu10I CCTNAGC 1 cut(s) 461
BpuEI CTTGAG 2 cut(s) 1171, 1288
Bsa29I ATCGAT 1 cut(s) 2508
BsaBI GATNNNNATC 1 cut(s) 156
BsaJI CCNNGG 7 cut(s) 16, 504, 642, 1173, 1687, 1947, 2388
BsaWI WCCGGW 2 cut(s) 116, 2228
Bsc4I CCNNNNNNNGG 1 cut(s) 1771
Bse118I RCCGGY 3 cut(s) 77, 116, 2228
Bse1I ACTGG 5 cut(s) 255, 332, 535, 1085, 1348
Bse3DI GCAATG 1 cut(s) 751
Bse8I GATNNNNATC 1 cut(s) 156
BseBI CCWGG 2 cut(s) 202, 1149
BseCI ATCGAT 1 cut(s) 2508
BseDI CCNNGG 7 cut(s) 16, 504, 642, 1173, 1687, 1947, 2388
BseGI GGATG 4 cut(s) 163, 1500, 1957, 2462
BseJI GATNNNNATC 1 cut(s) 156
BseLI CCNNNNNNNGG 1 cut(s) 1771
BseMI GCAATG 1 cut(s) 751
BseMII CTCAG 4 cut(s) 1310, 1548, 1632, 2381
BseNI ACTGG 5 cut(s) 255, 332, 535, 1085, 1348
BseRI GAGGAG 1 cut(s) 849
BseSI GKGCMC 2 cut(s) 852, 1102
BseXI GCAGC 3 cut(s) 673, 1897, 1967
Bsh1236I CGCG 2 cut(s) 600, 1040
Bsh1285I CGRYCG 1 cut(s) 577
BshFI GGCC 7 cut(s) 42, 319, 466, 850, 1147, 1536, 2422
BshNI GGYRCC 3 cut(s) 233, 2139, 2345
BshTI ACCGGT 2 cut(s) 116, 2228
BshVI ATCGAT 1 cut(s) 2508
BsiEI CGRYCG 1 cut(s) 577
BsiHKAI GWGCWC 2 cut(s) 378, 2239
BsiSI CCGG 3 cut(s) 78, 117, 2229
BslI CCNNNNNNNGG 1 cut(s) 1771
BsmAI GTCTC 2 cut(s) 1010, 1299
BsnI GGCC 7 cut(s) 42, 319, 466, 850, 1147, 1536, 2422
Bsp120I GGGCCC 1 cut(s) 848
Bsp1286I GDGCHC 4 cut(s) 378, 852, 1102, 2239
Bsp19I CCATGG 2 cut(s) 1687, 2388
BspACI CCGC 4 cut(s) 598, 896, 1383, 1668
BspANI GGCC 7 cut(s) 42, 319, 466, 850, 1147, 1536, 2422
BspCNI CTCAG 4 cut(s) 1309, 1549, 1631, 2380
BspDI ATCGAT 1 cut(s) 2508
BspFNI CGCG 2 cut(s) 600, 1040
BspLI GGNNCC 6 cut(s) 199, 235, 850, 1146, 2141, 2347
BspPI GGATC 4 cut(s) 1085, 1282, 1555, 1608
BspT107I GGYRCC 3 cut(s) 233, 2139, 2345
BsrDI GCAATG 1 cut(s) 751
BsrFI RCCGGY 3 cut(s) 77, 116, 2228
BsrI ACTGG 5 cut(s) 255, 332, 535, 1085, 1348
BssAI RCCGGY 3 cut(s) 77, 116, 2228
BssECI CCNNGG 7 cut(s) 16, 504, 642, 1173, 1687, 1947, 2388
BssNAI GTATAC 1 cut(s) 2194
BssSI CACGAG 1 cut(s) 1959
BssT1I CCWWGG 5 cut(s) 16, 504, 1173, 1687, 2388
Bst1107I GTATAC 1 cut(s) 2194
Bst2BI CACGAG 1 cut(s) 1959
Bst2UI CCWGG 2 cut(s) 202, 1149
Bst4CI ACNGT 6 cut(s) 38, 281, 337, 753, 1395, 2497
Bst6I CTCTTC 2 cut(s) 1657, 1672
BstC8I GCNNGC 3 cut(s) 1002, 1991, 2052
BstDEI CTNAG 5 cut(s) 461, 1296, 1557, 1618, 2367
BstDSI CCRYGG 3 cut(s) 1687, 1947, 2388
BstF5I GGATG 4 cut(s) 163, 1500, 1957, 2462
BstFNI CGCG 2 cut(s) 600, 1040
BstH2I RGCGCY 1 cut(s) 2103
BstHHI GCGC 1 cut(s) 2102
BstMAI GTCTC 2 cut(s) 1010, 1299
BstMCI CGRYCG 1 cut(s) 577
BstMWI GCNNNNNNNGC 4 cut(s) 27, 1952, 2060, 2372
BstNI CCWGG 2 cut(s) 202, 1149
BstNSI RCATGY 3 cut(s) 410, 769, 2397
BstPAI GACNNNNGTC 1 cut(s) 531
BstSCI CCNGG 2 cut(s) 200, 1147
BstSFI CTRYAG 3 cut(s) 1515, 1998, 2376
BstSLI GKGCMC 2 cut(s) 852, 1102
BstUI CGCG 2 cut(s) 600, 1040
BstV1I GCAGC 3 cut(s) 673, 1897, 1967
BstX2I RGATCY 4 cut(s) 1090, 1560, 1900, 2011
BstYI RGATCY 4 cut(s) 1090, 1560, 1900, 2011
BstZ17I GTATAC 1 cut(s) 2194
Bsu15I ATCGAT 1 cut(s) 2508
BsuI GTATCC 3 cut(s) 325, 567, 597
BsuRI GGCC 7 cut(s) 42, 319, 466, 850, 1147, 1536, 2422
BsuTUI ATCGAT 1 cut(s) 2508
BtgI CCRYGG 3 cut(s) 1687, 1947, 2388
BtgZI GCGATG 2 cut(s) 615, 2532
BtsCI GGATG 4 cut(s) 163, 1500, 1957, 2462
BtsIMutI CAGTG 3 cut(s) 342, 528, 1341
Cac8I GCNNGC 3 cut(s) 1002, 1991, 2052
CaiI CAGNNNCTG 1 cut(s) 207
CfoI GCGC 1 cut(s) 2102
Cfr10I RCCGGY 3 cut(s) 77, 116, 2228
Cfr13I GGNCC 5 cut(s) 317, 848, 849, 1145, 1534
ClaI ATCGAT 1 cut(s) 2508
CseI GACGC 4 cut(s) 211, 619, 1046, 1614
Csp6I GTAC 4 cut(s) 550, 681, 2140, 2346
CspAI ACCGGT 2 cut(s) 116, 2228
CviAII CATG 9 cut(s) 140, 407, 676, 766, 1095, 1688, 2308, 2389, 2394
CviQI GTAC 4 cut(s) 550, 681, 2140, 2346
DdeI CTNAG 5 cut(s) 461, 1296, 1557, 1618, 2367
DraI TTTAAA 1 cut(s) 1320
Eam1104I CTCTTC 2 cut(s) 1657, 1672
EarI CTCTTC 2 cut(s) 1657, 1672
EciI GGCGGA 1 cut(s) 1683
Ecl136II GAGCTC 1 cut(s) 376
Eco130I CCWWGG 5 cut(s) 16, 504, 1173, 1687, 2388
Eco147I AGGCCT 1 cut(s) 466
Eco24I GRGCYC 2 cut(s) 378, 852
Eco32I GATATC 1 cut(s) 1209
Eco53kI GAGCTC 1 cut(s) 376
Eco57I CTGAAG 1 cut(s) 2036
EcoICRI GAGCTC 1 cut(s) 376
EcoO109I RGGNCCY 2 cut(s) 849, 1145
EcoRI GAATTC 4 cut(s) 299, 435, 1567, 1760
EcoRII CCWGG 2 cut(s) 200, 1147
EcoRV GATATC 1 cut(s) 1209
EcoT14I CCWWGG 5 cut(s) 16, 504, 1173, 1687, 2388
EcoT38I GRGCYC 2 cut(s) 378, 852
ErhI CCWWGG 5 cut(s) 16, 504, 1173, 1687, 2388
FaeI CATG 9 cut(s) 143, 410, 679, 769, 1098, 1691, 2311, 2392, 2397
FalI AAGNNNNNCTT 4 cut(s) 1121, 1153, 1322, 1354
FatI CATG 9 cut(s) 139, 406, 675, 765, 1094, 1687, 2307, 2388, 2393
FauNDI CATATG 1 cut(s) 2276
FbaI TGATCA 2 cut(s) 246, 2482
FblI GTMKAC 2 cut(s) 1683, 2193
Fnu4HI GCNGC 5 cut(s) 687, 897, 1384, 1886, 1956
FokI GGATG 4 cut(s) 150, 1487, 1964, 2469
FriOI GRGCYC 2 cut(s) 378, 852
Fsp4HI GCNGC 5 cut(s) 687, 897, 1384, 1886, 1956
FspBI CTAG 3 cut(s) 807, 1472, 2216
GlaI GCGC 1 cut(s) 2101
GluI GCNGC 5 cut(s) 687, 897, 1384, 1886, 1956
HaeII RGCGCY 1 cut(s) 2103
HaeIII GGCC 7 cut(s) 42, 319, 466, 850, 1147, 1536, 2422
HapII CCGG 3 cut(s) 78, 117, 2229
HgaI GACGC 4 cut(s) 211, 619, 1046, 1614
HhaI GCGC 1 cut(s) 2102
Hin1II CATG 9 cut(s) 143, 410, 679, 769, 1098, 1691, 2311, 2392, 2397
Hin6I GCGC 1 cut(s) 2100
HinP1I GCGC 1 cut(s) 2100
HincII GTYRAC 1 cut(s) 1059
HindII GTYRAC 1 cut(s) 1059
HinfI GANTC 9 cut(s) 350, 710, 904, 1362, 1592, 1671, 1680, 2290, 2330
HpaII CCGG 3 cut(s) 78, 117, 2229
HphI GGTGA 5 cut(s) 131, 430, 1850, 2299, 2545
Hpy166II GTNNAC 4 cut(s) 681, 1059, 1684, 2194
Hpy188III TCNNGA 6 cut(s) 572, 807, 937, 1283, 1359, 1564
Hpy8I GTNNAC 4 cut(s) 681, 1059, 1684, 2194
Hpy99I CGWCG 4 cut(s) 227, 1031, 1608, 1835
HpyCH4III ACNGT 6 cut(s) 38, 281, 337, 753, 1395, 2497
HpyCH4IV ACGT 1 cut(s) 1914
HpyF10VI GCNNNNNNNGC 4 cut(s) 27, 1952, 2060, 2372
HpyF3I CTNAG 5 cut(s) 461, 1296, 1557, 1618, 2367
HpySE526I ACGT 1 cut(s) 1914
Hsp92II CATG 9 cut(s) 143, 410, 679, 769, 1098, 1691, 2311, 2392, 2397
HspAI GCGC 1 cut(s) 2100
KpnI GGTACC 2 cut(s) 2143, 2349
Ksp22I TGATCA 2 cut(s) 246, 2482
LmnI GCTCC 4 cut(s) 373, 795, 1009, 1520
Lsp1109I GCAGC 3 cut(s) 673, 1897, 1967
LweI GCATC 6 cut(s) 88, 90, 1658, 1942, 2110, 2137
MaeI CTAG 3 cut(s) 807, 1472, 2216
MaeII ACGT 1 cut(s) 1914
MaeIII GTNAC 9 cut(s) 653, 1299, 1725, 1823, 1838, 2287, 2488, 2497, 2551
MflI RGATCY 4 cut(s) 1090, 1560, 1900, 2011
MhlI GDGCHC 4 cut(s) 378, 852, 1102, 2239
MlyI GAGTC 5 cut(s) 913, 1356, 1601, 1689, 2284
MmeI TCCRAC 2 cut(s) 932, 1947
MroXI GAANNNNTTC 4 cut(s) 722, 1337, 1935, 2262
MseI TTAA 7 cut(s) 617, 828, 1319, 1397, 1539, 1776, 2036
MspA1I CMGCKG 1 cut(s) 1383
MspI CCGG 3 cut(s) 78, 117, 2229
MspR9I CCNGG 2 cut(s) 202, 1149
MvaI CCWGG 2 cut(s) 202, 1149
MvnI CGCG 2 cut(s) 600, 1040
MwoI GCNNNNNNNGC 4 cut(s) 27, 1952, 2060, 2372
NcoI CCATGG 2 cut(s) 1687, 2388
NdeI CATATG 1 cut(s) 2276
NlaIII CATG 9 cut(s) 143, 410, 679, 769, 1098, 1691, 2311, 2392, 2397
NlaIV GGNNCC 6 cut(s) 199, 235, 850, 1146, 2141, 2347
NmuCI GTSAC 3 cut(s) 1838, 2287, 2551
NspI RCATGY 3 cut(s) 410, 769, 2397
PceI AGGCCT 1 cut(s) 466
PciI ACATGT 1 cut(s) 406
PcsI WCGNNNNNNNCGW 1 cut(s) 2514
PdmI GAANNNNTTC 4 cut(s) 722, 1337, 1935, 2262
PfeI GAWTC 4 cut(s) 350, 710, 1671, 2330
PinAI ACCGGT 2 cut(s) 116, 2228
PkrI GCNGC 5 cut(s) 688, 898, 1385, 1887, 1957
Ple19I CGATCG 1 cut(s) 577
PleI GAGTC 5 cut(s) 912, 1356, 1600, 1688, 2284
PpsI GAGTC 5 cut(s) 912, 1356, 1600, 1688, 2284
PscI ACATGT 1 cut(s) 406
PshAI GACNNNNGTC 1 cut(s) 531
PsiI TTATAA 1 cut(s) 1065
Psp124BI GAGCTC 1 cut(s) 378
Psp6I CCWGG 2 cut(s) 200, 1147
PspGI CCWGG 2 cut(s) 200, 1147
PspN4I GGNNCC 6 cut(s) 199, 235, 850, 1146, 2141, 2347
PspOMI GGGCCC 1 cut(s) 848
PspPI GGNCC 5 cut(s) 317, 848, 849, 1145, 1534
PstNI CAGNNNCTG 1 cut(s) 207
PsuI RGATCY 4 cut(s) 1090, 1560, 1900, 2011
PvuI CGATCG 1 cut(s) 577
RsaI GTAC 4 cut(s) 551, 682, 2141, 2347
RsaNI GTAC 4 cut(s) 550, 681, 2140, 2346
SacI GAGCTC 1 cut(s) 378
SaqAI TTAA 7 cut(s) 617, 828, 1319, 1397, 1539, 1776, 2036
SatI GCNGC 5 cut(s) 687, 897, 1384, 1886, 1956
Sau96I GGNCC 5 cut(s) 317, 848, 849, 1145, 1534
SchI GAGTC 5 cut(s) 913, 1356, 1601, 1689, 2284
ScrFI CCNGG 2 cut(s) 202, 1149
SduI GDGCHC 4 cut(s) 378, 852, 1102, 2239
SfaNI GCATC 6 cut(s) 88, 90, 1658, 1942, 2110, 2137
SfcI CTRYAG 3 cut(s) 1515, 1998, 2376
SgrAI CRCCGGYG 1 cut(s) 116
SmlI CTYRAG 2 cut(s) 1186, 1267
SmoI CTYRAG 2 cut(s) 1186, 1267
SseBI AGGCCT 1 cut(s) 466
SsiI CCGC 4 cut(s) 598, 896, 1383, 1668
SspI AATATT 3 cut(s) 242, 721, 2156
SspMI CTAG 3 cut(s) 807, 1472, 2216
SstI GAGCTC 1 cut(s) 378
StuI AGGCCT 1 cut(s) 466
StyD4I CCNGG 2 cut(s) 200, 1147
StyI CCWWGG 5 cut(s) 16, 504, 1173, 1687, 2388
TaaI ACNGT 6 cut(s) 38, 281, 337, 753, 1395, 2497
TaiI ACGT 1 cut(s) 1917
TaqI TCGA 6 cut(s) 303, 876, 1603, 1801, 2508, 2534
TatI WGTACW 2 cut(s) 549, 680
TauI GCSGC 2 cut(s) 899, 1386
TfiI GAWTC 4 cut(s) 350, 710, 1671, 2330
Tru1I TTAA 7 cut(s) 617, 828, 1319, 1397, 1539, 1776, 2036
Tru9I TTAA 7 cut(s) 617, 828, 1319, 1397, 1539, 1776, 2036
TscAI CASTG 3 cut(s) 342, 535, 1348
TseFI GTSAC 3 cut(s) 1838, 2287, 2551
TseI GCWGC 3 cut(s) 686, 1885, 1955
Tsp45I GTSAC 3 cut(s) 1838, 2287, 2551
TspDTI ATGAA 6 cut(s) 13, 303, 501, 996, 2055, 2394
TspRI CASTG 3 cut(s) 342, 535, 1348
XapI RAATTY 9 cut(s) 52, 299, 435, 759, 1321, 1567, 1760, 1879, 2530
XbaI TCTAGA 1 cut(s) 806
XceI RCATGY 3 cut(s) 410, 769, 2397
XmiI GTMKAC 2 cut(s) 1683, 2193
XmnI GAANNNNTTC 4 cut(s) 722, 1337, 1935, 2262
XspI CTAG 3 cut(s) 807, 1472, 2216
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.