RLG00000020730

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000142
Physical Location & Seq
Forward (+)
68037084 .. 68038516
1433 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020730

Sequence Viewer

Length: 636 bp
ATGCTCCCATCACATTCCGCAGGTAAAGTCCTTAAAATAAAAGCAGTAGCCTCTTCAATTCCAACGGAAACAAGGTTGCAGCAGCCGCGGCGGCGGTGTTATGTATCTTGCCTTTTCTCTTGCCTTGACTTTGAAAAACAAGCTCAGTTGGCTTTTTGTGCCCCCGGTGATAAATCATGGACCTCCATTGAGGCCAAGGCGGCGGATTGGAATGATAAGGATATAGAAATAGTTGATGGGAAATTATATGCGGTAGGTAGTCACGCATTAGAGTTTTTAATGGTGTTTGACATTGAATTAGATACCAATGGCTGCAGAATTCATACCTGCACAGCGCAATGGTTGGTTACTCATCGCCCCAACTTTGAGAGCGTAGTTGAAGACACAGTTTACCATCGGATTTGTTACCTAGCAAAAGGTTCTGAATCAAATGAGTTGTTTATGATTTTGCATAAGCATCATCGTTACAGGACCAGAGGATTTCAAGTGTTGAAGTTGGAGTATAATGTTACGACTGGTCCTCAGTGGGTAGAGATTGTTGGCCTCGGGTGCCTGCAGAACCATAAGGTAGAGGATCGTAAAGGGCTTTTTGTTAACACGCCGGAAAGAGACCTTTTATCTTGGCCGGGACTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295

Protein Analysis

212

Amino Acids

24.08

Weight (kDa)

7.1

Isoelectric Point (pI)

37.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 43 - 183 2.2e-13 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000142)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g13532 FvH4_2g28870 FvH4_3g30930 FvH4_3g37281 FvH4_3g37300 FvH4_3g37301 FvH4_3g37301 FvH4_4g23711 FvH4_5g02330 FvH4_6g16960 FvH4_6g16971 FvH4_6g21291 FvH4_6g21331 FvH4_6g22212 FvH4_6g22332 FvH4_6g36920 FvH4_6g37552 FvH4_6g37591 FvH4_6g39721 FvH4_6g46020 FvH4_6g47001 FvH4_6g47002 FvH4_6g47130 FvH4_6g47140 FvH4_6g47161 FvH4_6g47170 FvH4_6g47190 FvH4_6g49120 FvH4_6g49280 FvH4_6g49281 FvH4_6g49640 FvH4_6g49640
malus_domestica MD03G1085600.v1.1 MD09G1027500.v1.1 MD09G1050100.v1.1
prunus_persica Prupe.1G004300_v2.0.a1 Prupe.1G016300_v2.0.a1 Prupe.1G019300_v2.0.a1 Prupe.3G239100_v2.0.a1 Prupe.3G291200_v2.0.a1
pyrus_communis pycom08g02840 pycom08g02860 pycom08g02870
rosa_chinensis RchiOBHm_Chr2g0153831 RchiOBHm_Chr2g0166171 RchiOBHm_Chr2g0166201 RchiOBHm_Chr3g0476221 RchiOBHm_Chr4g0430971 RchiOBHm_Chr5g0067081 RchiOBHm_Chr7g0197821
rosa_laevigata RLG00000001284 RLG00000006996 RLG00000012710 RLG00000019299 RLG00000020285 RLG00000020444 RLG00000020466 RLG00000020591 RLG00000020665 RLG00000020730 RLG00000020732 RLG00000021449 RLG00000021592 RLG00000021593 RLG00000021594 RLG00000021596 RLG00000021597 RLG00000021801 RLG00000023534 RLG00000023538 RLG00000023693 RLG00000023694 RLG00000023699 RLG00000024196 RLG00000024214 RLG00000024220 RLG00000024244 RLG00000024608 RLG00000024612 RLG00000035157 RLG00000035906
rosa_multiflora Rmu_co8282815.1_g000001 Rmu_co8380495.1_g000001 Rmu_co8381111.1_g000001 Rmu_co8460513.1_g000001 Rmu_co8481947.1_g000001 Rmu_co8487937.1_g000001 Rmu_sc0000151.1_g000017 Rmu_sc0001427.1_g000003 Rmu_sc0001427.1_g000006 Rmu_sc0002611.1_g000008 Rmu_sc0003887.1_g000018 Rmu_sc0003887.1_g000019 Rmu_sc0003887.1_g000021 Rmu_sc0004119.1_g000004 Rmu_sc0004329.1_g000003 Rmu_sc0004485.1_g000024 Rmu_sc0005290.1_g000001 Rmu_sc0005609.1_g000006 Rmu_sc0006187.1_g000018 Rmu_sc0006218.1_g000032 Rmu_sc0006218.1_g000033 Rmu_sc0007150.1_g000003 Rmu_sc0007154.1_g000004 Rmu_sc0007154.1_g000005 Rmu_sc0007154.1_g000007 Rmu_sc0008314.1_g000012 Rmu_sc0010073.1_g000023 Rmu_sc0010073.1_g000025 Rmu_sc0011216.1_g000008 Rmu_sc0012445.1_g000005 Rmu_sc0013571.1_g000002 Rmu_sc0018648.1_g000002 Rmu_sc0020961.1_g000002 Rmu_sc0020961.1_g000003 Rmu_sc0023710.1_g000001 Rmu_sc0031310.1_g000008 Rmu_sc0033940.1_g000001 Rmu_sc0035493.1_g000001 Rmu_sc0039285.1_g000001
rosa_roxburghii Rroxscaffold_1G00014000 Rroxscaffold_1G00014020 Rroxscaffold_2G00082760 Rroxscaffold_2G00085090 Rroxscaffold_2G00085100 Rroxscaffold_2G00085140 Rroxscaffold_2G00086670 Rroxscaffold_2G00086680 Rroxscaffold_2G00098190 Rroxscaffold_2G00099270 Rroxscaffold_3G00227460 Rroxscaffold_3G00258190 Rroxscaffold_3G00259780 Rroxscaffold_6G00402940 Rroxscaffold_6G00410200 Rroxscaffold_6G00410210 Rroxscaffold_7G00184320
rosa_rugosa Rorug02G0416100 Rorug02G0416200 Rorug02G0520900 Rorug02G0521200 Rorug02G0521300 Rorug02G0534600 Rorug03G0116300 Rorug03G0162900 Rorug03G0162900 Rorug04G0241400 Rorug05G0380900 Rorug07G0043800
rosa_samantha Rh1AG115300 Rh2BG483700 Rh2BG509900 Rh2BG515700 Rh2BG515800 Rh2BG586500 Rh2BG586600 Rh2BG586700 Rh2BG598800 Rh2BG598900 Rh2BG599100 Rh2BG618400 Rh2BG630500 Rh2CG457400 Rh2CG484900 Rh3AG165300 Rh3AG212900 Rh3AG213300 Rh3BG145800 Rh3BG146100 Rh3BG235300 Rh3BG246500 Rh3BG247000 Rh3BG247100 Rh3CG240300 Rh3CG240800 Rh3CG240900 Rh3CG259600 Rh3DG147000 Rh3DG184800 Rh3DG184900 Rh3DG185000 Rh3DG239500 Rh3DG240100 Rh3DG240200 Rh3DG255800 Rh5BG458500 Rh5CG480400 Rh7BG170400 Rh7BG407900 Rh7CG176900
rosa_wichuraiana Rw2G038990 Rw2G041550 Rw2G047660 Rw2G048940 Rw4G026370 Rw6G008730 Rw7G014600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 11, 335
AccB1I GGYRCC 1 cut(s) 549
AccII CGCG 1 cut(s) 88
AciI CCGC 8 cut(s) 18, 86, 88, 91, 94, 200, 203, 251
AclWI GGATC 1 cut(s) 582
AcoI YGGCCR 1 cut(s) 623
AcsI RAATTY 1 cut(s) 318
AgsI TTSAA 6 cut(s) 57, 134, 296, 380, 485, 493
AluBI AGCT 1 cut(s) 143
AluI AGCT 1 cut(s) 143
Alw26I GTCTC 1 cut(s) 603
AlwI GGATC 1 cut(s) 582
Ama87I CYCGRG 1 cut(s) 545
AoxI GGCC 3 cut(s) 192, 541, 623
ApeKI GCWGC 3 cut(s) 79, 82, 312
ApoI RAATTY 1 cut(s) 318
AspLEI GCGC 1 cut(s) 337
AspS9I GGNCC 3 cut(s) 180, 471, 518
AsuC2I CCSGG 2 cut(s) 165, 627
AsuHPI GGTGA 1 cut(s) 179
AvaI CYCGRG 1 cut(s) 545
AvaII GGWCC 3 cut(s) 180, 471, 518
BaeGI GKGCMC 1 cut(s) 163
BanI GGYRCC 1 cut(s) 549
BbsI GAAGAC 1 cut(s) 387
BbvI GCAGC 3 cut(s) 91, 94, 299
BccI CCATC 3 cut(s) 16, 230, 402
BcnI CCSGG 2 cut(s) 165, 627
BcoDI GTCTC 1 cut(s) 603
BfaI CTAG 1 cut(s) 410
BfmI CTRYAG 2 cut(s) 313, 554
BfuAI ACCTGC 2 cut(s) 11, 335
BglI GCCNNNNNGGC 2 cut(s) 91, 200
BisI GCNGC 7 cut(s) 80, 83, 86, 89, 92, 201, 313
BlsI GCNGC 7 cut(s) 81, 84, 87, 90, 93, 202, 314
Bme1390I CCNGG 2 cut(s) 165, 627
Bme18I GGWCC 3 cut(s) 180, 471, 518
BmeT110I CYCGRG 1 cut(s) 545
BmgT120I GGNCC 3 cut(s) 180, 471, 518
BmiI GGNNCC 1 cut(s) 551
BmrFI CCNGG 2 cut(s) 165, 627
BmsI GCATC 1 cut(s) 466
BpiI GAAGAC 1 cut(s) 387
BpuMI CCSGG 2 cut(s) 165, 627
BsaI GGTCTC 1 cut(s) 603
BsaJI CCNNGG 4 cut(s) 86, 163, 195, 544
Bse1I ACTGG 1 cut(s) 520
Bse3DI GCAATG 1 cut(s) 344
BseDI CCNNGG 4 cut(s) 86, 163, 195, 544
BseMI GCAATG 1 cut(s) 344
BseMII CTCAG 2 cut(s) 158, 536
BseNI ACTGG 1 cut(s) 520
BseSI GKGCMC 1 cut(s) 163
BseXI GCAGC 3 cut(s) 91, 94, 299
BsgI GTGCAG 1 cut(s) 313
Bsh1236I CGCG 1 cut(s) 88
BshFI GGCC 3 cut(s) 194, 543, 625
BshNI GGYRCC 1 cut(s) 549
BsiHKCI CYCGRG 1 cut(s) 545
BsiSI CCGG 3 cut(s) 165, 602, 626
BsmAI GTCTC 1 cut(s) 603
BsnI GGCC 3 cut(s) 194, 543, 625
Bso31I GGTCTC 1 cut(s) 603
BsoBI CYCGRG 1 cut(s) 545
Bsp1286I GDGCHC 1 cut(s) 163
Bsp143I GATC 1 cut(s) 574
BspACI CCGC 8 cut(s) 18, 86, 88, 91, 94, 200, 203, 251
BspANI GGCC 3 cut(s) 194, 543, 625
BspCNI CTCAG 2 cut(s) 157, 535
BspFNI CGCG 1 cut(s) 88
BspLI GGNNCC 1 cut(s) 551
BspMAI CTGCAG 2 cut(s) 317, 558
BspMI ACCTGC 2 cut(s) 11, 335
BspPI GGATC 1 cut(s) 582
BspT107I GGYRCC 1 cut(s) 549
BspTNI GGTCTC 1 cut(s) 603
BsrDI GCAATG 1 cut(s) 344
BsrI ACTGG 1 cut(s) 520
BssECI CCNNGG 4 cut(s) 86, 163, 195, 544
BssMI GATC 1 cut(s) 574
BssT1I CCWWGG 1 cut(s) 195
Bst4CI ACNGT 2 cut(s) 388, 633
Bst6I CTCTTC 1 cut(s) 58
BstC8I GCNNGC 1 cut(s) 554
BstDEI CTNAG 2 cut(s) 144, 522
BstDSI CCRYGG 1 cut(s) 86
BstFNI CGCG 1 cut(s) 88
BstHHI GCGC 1 cut(s) 337
BstKTI GATC 1 cut(s) 577
BstMAI GTCTC 1 cut(s) 603
BstMBI GATC 1 cut(s) 574
BstMWI GCNNNNNNNGC 7 cut(s) 85, 88, 91, 149, 158, 200, 549
BstSCI CCNGG 2 cut(s) 163, 625
BstSFI CTRYAG 2 cut(s) 313, 554
BstSLI GKGCMC 1 cut(s) 163
BstUI CGCG 1 cut(s) 88
BstV1I GCAGC 3 cut(s) 91, 94, 299
BstV2I GAAGAC 1 cut(s) 387
BsuRI GGCC 3 cut(s) 194, 543, 625
BtgI CCRYGG 1 cut(s) 86
BtgZI GCGATG 1 cut(s) 338
BtsIMutI CAGTG 1 cut(s) 530
BveI ACCTGC 2 cut(s) 11, 335
Cac8I GCNNGC 1 cut(s) 554
CfoI GCGC 1 cut(s) 337
Cfr13I GGNCC 3 cut(s) 180, 471, 518
Cfr42I CCGCGG 1 cut(s) 89
CviAII CATG 1 cut(s) 177
CviJI RGCY 9 cut(s) 50, 85, 143, 152, 194, 312, 543, 586, 625
CviKI_1 RGCY 9 cut(s) 50, 85, 143, 152, 194, 312, 543, 586, 625
DdeI CTNAG 2 cut(s) 144, 522
DpnI GATC 1 cut(s) 576
DpnII GATC 1 cut(s) 574
EaeI YGGCCR 1 cut(s) 623
Eam1104I CTCTTC 1 cut(s) 58
EarI CTCTTC 1 cut(s) 58
EciI GGCGGA 1 cut(s) 218
Eco130I CCWWGG 1 cut(s) 195
Eco31I GGTCTC 1 cut(s) 603
Eco47I GGWCC 3 cut(s) 180, 471, 518
Eco88I CYCGRG 1 cut(s) 545
EcoRI GAATTC 1 cut(s) 318
EcoT14I CCWWGG 1 cut(s) 195
ErhI CCWWGG 1 cut(s) 195
FaeI CATG 1 cut(s) 180
FatI CATG 1 cut(s) 176
Fnu4HI GCNGC 7 cut(s) 80, 83, 86, 89, 92, 201, 313
Fsp4HI GCNGC 7 cut(s) 80, 83, 86, 89, 92, 201, 313
FspBI CTAG 1 cut(s) 410
GlaI GCGC 1 cut(s) 336
GluI GCNGC 7 cut(s) 80, 83, 86, 89, 92, 201, 313
HaeIII GGCC 3 cut(s) 194, 543, 625
HapII CCGG 3 cut(s) 165, 602, 626
HhaI GCGC 1 cut(s) 337
Hin1II CATG 1 cut(s) 180
Hin6I GCGC 1 cut(s) 335
HinP1I GCGC 1 cut(s) 335
HincII GTYRAC 1 cut(s) 595
HindII GTYRAC 1 cut(s) 595
HinfI GANTC 1 cut(s) 425
HpaI GTTAAC 1 cut(s) 595
HpaII CCGG 3 cut(s) 165, 602, 626
HphI GGTGA 1 cut(s) 179
Hpy166II GTNNAC 2 cut(s) 391, 595
Hpy188I TCNGA 2 cut(s) 399, 424
Hpy8I GTNNAC 2 cut(s) 391, 595
HpyCH4III ACNGT 2 cut(s) 388, 633
HpyCH4V TGCA 5 cut(s) 79, 315, 330, 451, 556
HpyF10VI GCNNNNNNNGC 7 cut(s) 85, 88, 91, 149, 158, 200, 549
HpyF3I CTNAG 2 cut(s) 144, 522
Hsp92II CATG 1 cut(s) 180
HspAI GCGC 1 cut(s) 335
KspAI GTTAAC 1 cut(s) 595
KspI CCGCGG 1 cut(s) 89
Kzo9I GATC 1 cut(s) 574
LmnI GCTCC 1 cut(s) 9
LpnPI CCDG 8 cut(s) 6, 178, 340, 454, 487, 501, 566, 615
Lsp1109I GCAGC 3 cut(s) 91, 94, 299
LweI GCATC 1 cut(s) 466
MaeI CTAG 1 cut(s) 410
MaeIII GTNAC 5 cut(s) 260, 346, 404, 464, 508
MalI GATC 1 cut(s) 576
MboI GATC 1 cut(s) 574
MboII GAAGA 2 cut(s) 45, 392
MhlI GDGCHC 1 cut(s) 163
MluCI AATT 4 cut(s) 57, 242, 296, 318
MmeI TCCRAC 2 cut(s) 86, 477
MnlI CCTC 7 cut(s) 61, 184, 193, 470, 531, 554, 565
MseI TTAA 3 cut(s) 33, 278, 594
MspA1I CMGCKG 1 cut(s) 88
MspI CCGG 3 cut(s) 165, 602, 626
MspR9I CCNGG 2 cut(s) 165, 627
MvnI CGCG 1 cut(s) 88
MwoI GCNNNNNNNGC 7 cut(s) 85, 88, 91, 149, 158, 200, 549
NciI CCSGG 2 cut(s) 165, 627
NdeII GATC 1 cut(s) 574
NlaIII CATG 1 cut(s) 180
NlaIV GGNNCC 1 cut(s) 551
NmuCI GTSAC 1 cut(s) 260
PfeI GAWTC 1 cut(s) 425
PkrI GCNGC 7 cut(s) 81, 84, 87, 90, 93, 202, 314
PspN4I GGNNCC 1 cut(s) 551
PspPI GGNCC 3 cut(s) 180, 471, 518
PstI CTGCAG 2 cut(s) 317, 558
SacII CCGCGG 1 cut(s) 89
SaqAI TTAA 3 cut(s) 33, 278, 594
SatI GCNGC 7 cut(s) 80, 83, 86, 89, 92, 201, 313
Sau3AI GATC 1 cut(s) 574
Sau96I GGNCC 3 cut(s) 180, 471, 518
ScrFI CCNGG 2 cut(s) 165, 627
SduI GDGCHC 1 cut(s) 163
SfaNI GCATC 1 cut(s) 466
SfcI CTRYAG 2 cut(s) 313, 554
Sfr303I CCGCGG 1 cut(s) 89
SgrBI CCGCGG 1 cut(s) 89
SinI GGWCC 3 cut(s) 180, 471, 518
Sse9I AATT 4 cut(s) 57, 242, 296, 318
SsiI CCGC 8 cut(s) 18, 86, 88, 91, 94, 200, 203, 251
SspMI CTAG 1 cut(s) 410
StyD4I CCNGG 2 cut(s) 163, 625
StyI CCWWGG 1 cut(s) 195
TaaI ACNGT 2 cut(s) 388, 633
TasI AATT 4 cut(s) 57, 242, 296, 318
TauI GCSGC 4 cut(s) 88, 91, 94, 203
TfiI GAWTC 1 cut(s) 425
Tru1I TTAA 3 cut(s) 33, 278, 594
Tru9I TTAA 3 cut(s) 33, 278, 594
TscAI CASTG 1 cut(s) 530
TseFI GTSAC 1 cut(s) 260
TseI GCWGC 3 cut(s) 79, 82, 312
Tsp45I GTSAC 1 cut(s) 260
TspDTI ATGAA 1 cut(s) 311
TspGWI ACGGA 1 cut(s) 80
TspRI CASTG 1 cut(s) 530
VpaK11BI GGWCC 3 cut(s) 180, 471, 518
XapI RAATTY 1 cut(s) 318
XspI CTAG 1 cut(s) 410
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.