Rorug02G0534600

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000142
Physical Location & Seq
Forward (+)
66379689 .. 66381899
2211 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0534600.1

Sequence Viewer

Length: 1482 bp
ATGCCCAAACCCCTAACTCCCTCCCGCCTCAAGGCCCTCCTCTTATTCCTCTCCCTCTTCCTCAACCTCTACCTCCTCCTCTCCCCCCACGCGCCTCAGTTCCTCCTCACGCGCTACTCCTCCTCTTCCTCCTCATCCTCACCCTCACCCACCACGCGCCGCCACCTCCTCTTCGCCATCGCGTCCTCCTCACGCTCCTGGACCCGCCGCAAGCCCTACCTCCGCCTCTGGCACTCCCCCGCCTCCACCCGCGCCTTCGCCTTCCTCGACCGCCCCGCCGACCCCGCCGACGACGACGGCGGCGCCGCGCCTGTGATCGTCTCCGGCGACACCTCCCGGTTCCCGTACACTCTCCGCGGCGGGCTCCGGTCCGCGATCCGCGTGGCGCGCGTGGTCAAGGAGATCGTCGATCGCGGCGAGGCGGACGTCCGGTGGTACGTGTTCGGCGACGACGACACGGTGTTCTTCGTCGACAATTTGGTGAAGACGCTGTCAAAGTACGATCACGATCGGTGGTTCTACATCGGGAGCAACTCGGAGAGCTACATGCAGAATGTCAAGTACGGGTTCGACATGGCGTTTGGCGGCGGCGGATTCGCCATCAGCCATTCTCTGGCTAAGGTTTTGGCTAGGGTTTTCGACTCGTGCTTGATGAGGTACGGCCACTTGTATGGAAGTGATTCCCGCGTTTTTTCGTGCGTGGCGGAGCTCGGCATTGGGTTAACCCATGAACCTGGGTTTCATCAGGTTGATATGCGGGGTAATTTGTTTGGAATGCTATCTGCACATCCATTGTCACCTTTGGTTTCGCTTCACCATTTGGATGCTGCAGATCCGATCTTCCCCAACATGAACAACACTCAAGCTTTGGAACATCTTTTTGAAGCTGTGAATGCTGATCCTGCCAGGATATTGCAGCAAACTGTATGCTATGACCGCACACATTCATTGACTGTTTCGGTTGCATGGGGTTATGCCATACAGGTGTATGATGGCAATGAACTTCTTCCAGATCTCCTTTCACTGCAGAAAACTTTTATGCCATGGAGGAGGAGCCGTAGTGTTGAGGCAAGTCAGTACATGTTCAAAATGAGAGACTATCCTAGAGATGCATGTAAAAGACCCCCTATATTTTATTTGGAAAGCCTCGTCTCTAATAACCATGGCATCTGGAGCAACTACACCAGGCACAATGTGGAAAACTGCTCCAAAGCAAATGCAATAAAGAACCTGGAACATATCAGAGTAATTTCACAGAAGCTAGACCTTGATGTTGAAGAGATGAAGGCTCCACGTCGTCAGTGCTGCGACATTTTGCAATCTTACATGCAGAATTCAGAGAATTTGAAGCAATTCTGGTTTACTGTGGAATCTTTTAAAGTGCTTGAGATTCCTAAAGAAGAGGACGATATGCTGGGAGATAACCATTTGCCTTCAAATCAACACATGGAGCAAAATTGGAAAATTAATGATGTTGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295

Protein Analysis

493

Amino Acids

56.22

Weight (kDa)

7.01

Isoelectric Point (pI)

55.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fringe PF02434 51 - 250 8.8e-15 Fringe-like
DUF604 PF04646 184 - 436 3.6e-103 Protein of unknown function, DUF604
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000142)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g13532 FvH4_2g28870 FvH4_3g30930 FvH4_3g37281 FvH4_3g37300 FvH4_3g37301 FvH4_3g37301 FvH4_4g23711 FvH4_5g02330 FvH4_6g16960 FvH4_6g16971 FvH4_6g21291 FvH4_6g21331 FvH4_6g22212 FvH4_6g22332 FvH4_6g36920 FvH4_6g37552 FvH4_6g37591 FvH4_6g39721 FvH4_6g46020 FvH4_6g47001 FvH4_6g47002 FvH4_6g47130 FvH4_6g47140 FvH4_6g47161 FvH4_6g47170 FvH4_6g47190 FvH4_6g49120 FvH4_6g49280 FvH4_6g49281 FvH4_6g49640 FvH4_6g49640
malus_domestica MD03G1085600.v1.1 MD09G1027500.v1.1 MD09G1050100.v1.1
prunus_persica Prupe.1G004300_v2.0.a1 Prupe.1G016300_v2.0.a1 Prupe.1G019300_v2.0.a1 Prupe.3G239100_v2.0.a1 Prupe.3G291200_v2.0.a1
pyrus_communis pycom08g02840 pycom08g02860 pycom08g02870
rosa_chinensis RchiOBHm_Chr2g0153831 RchiOBHm_Chr2g0166171 RchiOBHm_Chr2g0166201 RchiOBHm_Chr3g0476221 RchiOBHm_Chr4g0430971 RchiOBHm_Chr5g0067081 RchiOBHm_Chr7g0197821
rosa_laevigata RLG00000001284 RLG00000006996 RLG00000012710 RLG00000019299 RLG00000020285 RLG00000020444 RLG00000020466 RLG00000020591 RLG00000020665 RLG00000020730 RLG00000020732 RLG00000021449 RLG00000021592 RLG00000021593 RLG00000021594 RLG00000021596 RLG00000021597 RLG00000021801 RLG00000023534 RLG00000023538 RLG00000023693 RLG00000023694 RLG00000023699 RLG00000024196 RLG00000024214 RLG00000024220 RLG00000024244 RLG00000024608 RLG00000024612 RLG00000035157 RLG00000035906
rosa_multiflora Rmu_co8282815.1_g000001 Rmu_co8380495.1_g000001 Rmu_co8381111.1_g000001 Rmu_co8460513.1_g000001 Rmu_co8481947.1_g000001 Rmu_co8487937.1_g000001 Rmu_sc0000151.1_g000017 Rmu_sc0001427.1_g000003 Rmu_sc0001427.1_g000006 Rmu_sc0002611.1_g000008 Rmu_sc0003887.1_g000018 Rmu_sc0003887.1_g000019 Rmu_sc0003887.1_g000021 Rmu_sc0004119.1_g000004 Rmu_sc0004329.1_g000003 Rmu_sc0004485.1_g000024 Rmu_sc0005290.1_g000001 Rmu_sc0005609.1_g000006 Rmu_sc0006187.1_g000018 Rmu_sc0006218.1_g000032 Rmu_sc0006218.1_g000033 Rmu_sc0007150.1_g000003 Rmu_sc0007154.1_g000004 Rmu_sc0007154.1_g000005 Rmu_sc0007154.1_g000007 Rmu_sc0008314.1_g000012 Rmu_sc0010073.1_g000023 Rmu_sc0010073.1_g000025 Rmu_sc0011216.1_g000008 Rmu_sc0012445.1_g000005 Rmu_sc0013571.1_g000002 Rmu_sc0018648.1_g000002 Rmu_sc0020961.1_g000002 Rmu_sc0020961.1_g000003 Rmu_sc0023710.1_g000001 Rmu_sc0031310.1_g000008 Rmu_sc0033940.1_g000001 Rmu_sc0035493.1_g000001 Rmu_sc0039285.1_g000001
rosa_roxburghii Rroxscaffold_1G00014000 Rroxscaffold_1G00014020 Rroxscaffold_2G00082760 Rroxscaffold_2G00085090 Rroxscaffold_2G00085100 Rroxscaffold_2G00085140 Rroxscaffold_2G00086670 Rroxscaffold_2G00086680 Rroxscaffold_2G00098190 Rroxscaffold_2G00099270 Rroxscaffold_3G00227460 Rroxscaffold_3G00258190 Rroxscaffold_3G00259780 Rroxscaffold_6G00402940 Rroxscaffold_6G00410200 Rroxscaffold_6G00410210 Rroxscaffold_7G00184320
rosa_rugosa Rorug02G0416100 Rorug02G0416200 Rorug02G0520900 Rorug02G0521200 Rorug02G0521300 Rorug02G0534600 Rorug03G0116300 Rorug03G0162900 Rorug03G0162900 Rorug04G0241400 Rorug05G0380900 Rorug07G0043800
rosa_samantha Rh1AG115300 Rh2BG483700 Rh2BG509900 Rh2BG515700 Rh2BG515800 Rh2BG586500 Rh2BG586600 Rh2BG586700 Rh2BG598800 Rh2BG598900 Rh2BG599100 Rh2BG618400 Rh2BG630500 Rh2CG457400 Rh2CG484900 Rh3AG165300 Rh3AG212900 Rh3AG213300 Rh3BG145800 Rh3BG146100 Rh3BG235300 Rh3BG246500 Rh3BG247000 Rh3BG247100 Rh3CG240300 Rh3CG240800 Rh3CG240900 Rh3CG259600 Rh3DG147000 Rh3DG184800 Rh3DG184900 Rh3DG185000 Rh3DG239500 Rh3DG240100 Rh3DG240200 Rh3DG255800 Rh5BG458500 Rh5CG480400 Rh7BG170400 Rh7BG407900 Rh7CG176900
rosa_wichuraiana Rw2G038990 Rw2G041550 Rw2G047660 Rw2G048940 Rw4G026370 Rw6G008730 Rw7G014600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 429
AccB1I GGYRCC 1 cut(s) 302
AccB7I CCANNNNNTGG 1 cut(s) 613
AccI GTMKAC 1 cut(s) 471
AclWI GGATC 3 cut(s) 370, 827, 893
AcoI YGGCCR 1 cut(s) 661
AcsI RAATTY 2 cut(s) 1333, 1342
AcyI GRCGYC 2 cut(s) 303, 426
AdeI CACNNNGTG 1 cut(s) 1195
AfaI GTAC 6 cut(s) 347, 437, 500, 563, 659, 1079
AfiI CCNNNNNNNGG 2 cut(s) 31, 613
AflIII ACRYGT 2 cut(s) 438, 1080
AgsI TTSAA 6 cut(s) 884, 1087, 1277, 1348, 1437, 1478
AjiI CACGTC 1 cut(s) 1295
AjnI CCWGG 5 cut(s) 197, 733, 905, 1184, 1230
AluBI AGCT 5 cut(s) 543, 709, 866, 887, 1261
AluI AGCT 5 cut(s) 543, 709, 866, 887, 1261
Alw21I GWGCWC 1 cut(s) 711
Alw26I GTCTC 3 cut(s) 325, 1089, 1156
AlwI GGATC 3 cut(s) 370, 827, 893
AoxI GGCC 2 cut(s) 33, 661
ApeKI GCWGC 3 cut(s) 827, 916, 1305
ApoI RAATTY 2 cut(s) 1333, 1342
ArsI GACNNNNNNTTYG 2 cut(s) 563, 595
AseI ATTAAT 1 cut(s) 1467
Asp700I GAANNNNTTC 3 cut(s) 679, 1005, 1352
AspLEI GCGC 8 cut(s) 94, 114, 159, 254, 305, 310, 388, 390
AspS9I GGNCC 3 cut(s) 34, 201, 369
AsuC2I CCSGG 1 cut(s) 337
AsuHPI GGTGA 5 cut(s) 132, 138, 493, 789, 806
AvaII GGWCC 2 cut(s) 201, 369
BanI GGYRCC 1 cut(s) 302
BanII GRGCYC 2 cut(s) 366, 711
BauI CACGAG 1 cut(s) 643
BbsI GAAGAC 1 cut(s) 491
Bbv12I GWGCWC 1 cut(s) 711
BbvI GCAGC 3 cut(s) 814, 928, 1292
BccI CCATC 3 cut(s) 185, 608, 986
BceAI ACGGC 3 cut(s) 313, 676, 1041
BciT130I CCWGG 5 cut(s) 199, 735, 907, 1186, 1232
BcnI CCSGG 1 cut(s) 337
BcoDI GTCTC 3 cut(s) 325, 1089, 1156
BfaI CTAG 3 cut(s) 630, 1104, 1262
BfmI CTRYAG 2 cut(s) 828, 1025
BfoI RGCGCY 1 cut(s) 306
BglII AGATCT 1 cut(s) 1012
Bme1390I CCNGG 6 cut(s) 199, 337, 735, 907, 1186, 1232
Bme18I GGWCC 2 cut(s) 201, 369
BmgBI CACGTC 1 cut(s) 1295
BmgT120I GGNCC 3 cut(s) 34, 201, 369
BmiI GGNNCC 6 cut(s) 203, 304, 341, 365, 1055, 1290
BmrFI CCNGG 6 cut(s) 199, 337, 735, 907, 1186, 1232
BmsI GCATC 3 cut(s) 814, 1099, 1176
BpiI GAAGAC 1 cut(s) 491
BpmI CTGGAG 1 cut(s) 1192
Bpu10I CCTNAGC 1 cut(s) 618
BpuEI CTTGAG 3 cut(s) 14, 846, 1406
BpuMI CCSGG 1 cut(s) 337
BsaAI YACGTR 1 cut(s) 439
BsaBI GATNNNNATC 1 cut(s) 507
BsaHI GRCGYC 2 cut(s) 303, 426
BsaJI CCNNGG 4 cut(s) 355, 734, 1043, 1162
BsaWI WCCGGW 2 cut(s) 366, 429
BsaXI ACNNNNNCTCC 4 cut(s) 1042, 1045, 1072, 1075
Bsc4I CCNNNNNNNGG 2 cut(s) 31, 613
Bse3DI GCAATG 1 cut(s) 1003
Bse8I GATNNNNATC 1 cut(s) 507
BseBI CCWGG 5 cut(s) 199, 735, 907, 1186, 1232
BseDI CCNNGG 4 cut(s) 355, 734, 1043, 1162
BseGI GGATG 3 cut(s) 134, 787, 829
BseJI GATNNNNATC 1 cut(s) 507
BseLI CCNNNNNNNGG 2 cut(s) 31, 613
BseMI GCAATG 1 cut(s) 1003
BseMII CTCAG 1 cut(s) 110
BsePI GCGCGC 1 cut(s) 386
BseXI GCAGC 3 cut(s) 814, 928, 1292
BseYI CCCAGC 1 cut(s) 1414
BsgI GTGCAG 1 cut(s) 768
Bsh1285I CGRYCG 3 cut(s) 271, 412, 511
BshFI GGCC 2 cut(s) 35, 663
BshNI GGYRCC 1 cut(s) 302
BsiEI CGRYCG 3 cut(s) 271, 412, 511
BsiHKAI GWGCWC 1 cut(s) 711
BsiSI CCGG 4 cut(s) 324, 337, 367, 430
BslI CCNNNNNNNGG 2 cut(s) 31, 613
BsmAI GTCTC 3 cut(s) 325, 1089, 1156
BsmBI CGTCTC 2 cut(s) 325, 1156
BsmI GAATGC 2 cut(s) 780, 898
BsnI GGCC 2 cut(s) 35, 663
Bsp1286I GDGCHC 2 cut(s) 366, 711
Bsp19I CCATGG 2 cut(s) 1043, 1162
BspANI GGCC 2 cut(s) 35, 663
BspCNI CTCAG 1 cut(s) 109
BspLI GGNNCC 6 cut(s) 203, 304, 341, 365, 1055, 1290
BspMAI CTGCAG 2 cut(s) 832, 1029
BspPI GGATC 3 cut(s) 370, 827, 893
BspT107I GGYRCC 1 cut(s) 302
BsrDI GCAATG 1 cut(s) 1003
BssECI CCNNGG 4 cut(s) 355, 734, 1043, 1162
BssHII GCGCGC 1 cut(s) 386
BssNI GRCGYC 2 cut(s) 303, 426
BssSI CACGAG 1 cut(s) 643
BssT1I CCWWGG 2 cut(s) 1043, 1162
Bst2BI CACGAG 1 cut(s) 643
Bst2UI CCWGG 5 cut(s) 199, 735, 907, 1186, 1232
Bst4CI ACNGT 4 cut(s) 460, 925, 955, 1366
Bst6I CTCTTC 5 cut(s) 62, 130, 176, 1272, 1395
BstACI GRCGYC 2 cut(s) 303, 426
BstBAI YACGTR 1 cut(s) 439
BstC8I GCNNGC 3 cut(s) 212, 362, 388
BstDEI CTNAG 2 cut(s) 96, 618
BstDSI CCRYGG 3 cut(s) 355, 1043, 1162
BstF5I GGATG 3 cut(s) 134, 787, 829
BstH2I RGCGCY 1 cut(s) 306
BstHHI GCGC 8 cut(s) 94, 114, 159, 254, 305, 310, 388, 390
BstMAI GTCTC 3 cut(s) 325, 1089, 1156
BstMCI CGRYCG 3 cut(s) 271, 412, 511
BstMWI GCNNNNNNNGC 6 cut(s) 284, 387, 893, 902, 936, 1173
BstNI CCWGG 5 cut(s) 199, 735, 907, 1186, 1232
BstNSI RCATGY 4 cut(s) 550, 1084, 1116, 1330
BstSCI CCNGG 6 cut(s) 197, 335, 733, 905, 1184, 1230
BstSFI CTRYAG 2 cut(s) 828, 1025
BstV1I GCAGC 3 cut(s) 814, 928, 1292
BstV2I GAAGAC 1 cut(s) 491
BstX2I RGATCY 2 cut(s) 832, 1012
BstXI CCANNNNNNTGG 2 cut(s) 671, 734
BstYI RGATCY 2 cut(s) 832, 1012
BsuRI GGCC 2 cut(s) 35, 663
BtgI CCRYGG 3 cut(s) 355, 1043, 1162
BtgZI GCGATG 1 cut(s) 163
BtrI CACGTC 1 cut(s) 1295
BtsCI GGATG 3 cut(s) 134, 787, 829
BtsI GCAGTG 1 cut(s) 1022
BtsIMutI CAGTG 2 cut(s) 1022, 1307
Cac8I GCNNGC 3 cut(s) 212, 362, 388
CfoI GCGC 8 cut(s) 94, 114, 159, 254, 305, 310, 388, 390
Cfr13I GGNCC 3 cut(s) 34, 201, 369
Cfr42I CCGCGG 1 cut(s) 358
CpoI CGGWCCG 1 cut(s) 369
CseI GACGC 2 cut(s) 171, 496
Csp6I GTAC 6 cut(s) 346, 436, 499, 562, 658, 1078
CspI CGGWCCG 1 cut(s) 369
CviQI GTAC 6 cut(s) 346, 436, 499, 562, 658, 1078
DdeI CTNAG 2 cut(s) 96, 618
DinI GGCGCC 1 cut(s) 304
DraI TTTAAA 1 cut(s) 1378
DraIII CACNNNGTG 1 cut(s) 1195
EaeI YGGCCR 1 cut(s) 661
Eam1104I CTCTTC 5 cut(s) 62, 130, 176, 1272, 1395
EarI CTCTTC 5 cut(s) 62, 130, 176, 1272, 1395
EciI GGCGGA 4 cut(s) 212, 437, 606, 719
Ecl136II GAGCTC 1 cut(s) 709
Eco130I CCWWGG 2 cut(s) 1043, 1162
Eco24I GRGCYC 2 cut(s) 366, 711
Eco47I GGWCC 2 cut(s) 201, 369
Eco53kI GAGCTC 1 cut(s) 709
EcoICRI GAGCTC 1 cut(s) 709
EcoO109I RGGNCCY 1 cut(s) 34
EcoRI GAATTC 1 cut(s) 1333
EcoRII CCWGG 5 cut(s) 197, 733, 905, 1184, 1230
EcoT14I CCWWGG 2 cut(s) 1043, 1162
EcoT22I ATGCAT 1 cut(s) 1114
EcoT38I GRGCYC 2 cut(s) 366, 711
EgeI GGCGCC 1 cut(s) 304
EheI GGCGCC 1 cut(s) 304
ErhI CCWWGG 2 cut(s) 1043, 1162
Esp3I CGTCTC 2 cut(s) 325, 1156
FauI CCCGC 9 cut(s) 32, 212, 247, 257, 283, 292, 353, 692, 750
FblI GTMKAC 1 cut(s) 471
FokI GGATG 3 cut(s) 121, 774, 836
FriOI GRGCYC 2 cut(s) 366, 711
FspBI CTAG 3 cut(s) 630, 1104, 1262
GlaI GCGC 8 cut(s) 93, 113, 158, 253, 304, 309, 387, 389
GsaI CCCAGC 1 cut(s) 1418
GsuI CTGGAG 1 cut(s) 1192
HaeII RGCGCY 1 cut(s) 306
HaeIII GGCC 2 cut(s) 35, 663
HapII CCGG 4 cut(s) 324, 337, 367, 430
HgaI GACGC 2 cut(s) 171, 496
HhaI GCGC 8 cut(s) 94, 114, 159, 254, 305, 310, 388, 390
Hin1I GRCGYC 2 cut(s) 303, 426
Hin6I GCGC 8 cut(s) 92, 112, 157, 252, 303, 308, 386, 388
HinP1I GCGC 8 cut(s) 92, 112, 157, 252, 303, 308, 386, 388
HincII GTYRAC 2 cut(s) 472, 723
HindII GTYRAC 2 cut(s) 472, 723
HindIII AAGCTT 1 cut(s) 864
HinfI GANTC 5 cut(s) 594, 641, 680, 1370, 1390
HpaI GTTAAC 1 cut(s) 723
HpaII CCGG 4 cut(s) 324, 337, 367, 430
HphI GGTGA 5 cut(s) 132, 138, 493, 789, 806
Hpy166II GTNNAC 4 cut(s) 348, 472, 723, 1362
Hpy188I TCNGA 4 cut(s) 538, 837, 1244, 1339
Hpy188III TCNNGA 4 cut(s) 506, 526, 1010, 1171
Hpy8I GTNNAC 4 cut(s) 348, 472, 723, 1362
Hpy99I CGWCG 8 cut(s) 293, 296, 299, 410, 452, 455, 473, 1299
HpyAV CCTTC 4 cut(s) 265, 271, 1279, 1443
HpyCH4III ACNGT 4 cut(s) 460, 925, 955, 1366
HpyCH4IV ACGT 3 cut(s) 426, 438, 1294
HpyF10VI GCNNNNNNNGC 6 cut(s) 284, 387, 893, 902, 936, 1173
HpyF3I CTNAG 2 cut(s) 96, 618
HpySE526I ACGT 3 cut(s) 426, 438, 1294
Hsp92I GRCGYC 2 cut(s) 303, 426
HspAI GCGC 8 cut(s) 92, 112, 157, 252, 303, 308, 386, 388
KasI GGCGCC 1 cut(s) 302
KspAI GTTAAC 1 cut(s) 723
KspI CCGCGG 1 cut(s) 358
LmnI GCTCC 9 cut(s) 200, 369, 528, 706, 1053, 1173, 1211, 1294, 1450
Lsp1109I GCAGC 3 cut(s) 814, 928, 1292
LweI GCATC 3 cut(s) 814, 1099, 1176
MaeI CTAG 3 cut(s) 630, 1104, 1262
MaeII ACGT 3 cut(s) 426, 438, 1294
MaeIII GTNAC 1 cut(s) 795
MboII GAAGA 9 cut(s) 49, 117, 163, 457, 496, 832, 998, 1289, 1412
MflI RGATCY 2 cut(s) 832, 1012
MhlI GDGCHC 2 cut(s) 366, 711
MluCI AATT 8 cut(s) 475, 763, 1248, 1333, 1342, 1352, 1456, 1464
Mly113I GGCGCC 1 cut(s) 303
MlyI GAGTC 1 cut(s) 635
Mph1103I ATGCAT 1 cut(s) 1114
MroXI GAANNNNTTC 3 cut(s) 679, 1005, 1352
MseI TTAA 3 cut(s) 722, 1377, 1467
MslI CAYNNNNRTG 3 cut(s) 669, 822, 983
MspA1I CMGCKG 1 cut(s) 357
MspI CCGG 4 cut(s) 324, 337, 367, 430
MspR9I CCNGG 6 cut(s) 199, 337, 735, 907, 1186, 1232
MteI GCGCNGCGC 1 cut(s) 306
Mva1269I GAATGC 2 cut(s) 780, 898
MvaI CCWGG 5 cut(s) 199, 735, 907, 1186, 1232
MwoI GCNNNNNNNGC 6 cut(s) 284, 387, 893, 902, 936, 1173
NarI GGCGCC 1 cut(s) 303
NciI CCSGG 1 cut(s) 337
NcoI CCATGG 2 cut(s) 1043, 1162
NlaIV GGNNCC 6 cut(s) 203, 304, 341, 365, 1055, 1290
NmeAIII GCCGAG 1 cut(s) 690
NmuCI GTSAC 1 cut(s) 795
NsiI ATGCAT 1 cut(s) 1114
NspI RCATGY 4 cut(s) 550, 1084, 1116, 1330
PauI GCGCGC 1 cut(s) 386
PciI ACATGT 1 cut(s) 1080
PcsI WCGNNNNNNNCGW 5 cut(s) 264, 324, 414, 444, 450
PctI GAATGC 2 cut(s) 780, 898
PdmI GAANNNNTTC 3 cut(s) 679, 1005, 1352
PfeI GAWTC 4 cut(s) 594, 680, 1370, 1390
PflFI GACNNNGTC 1 cut(s) 490
PflMI CCANNNNNTGG 1 cut(s) 613
PfoI TCCNGGA 1 cut(s) 197
Ple19I CGATCG 2 cut(s) 412, 511
PleI GAGTC 1 cut(s) 635
PluTI GGCGCC 1 cut(s) 306
PpsI GAGTC 1 cut(s) 635
Ppu21I YACGTR 1 cut(s) 439
PscI ACATGT 1 cut(s) 1080
PshBI ATTAAT 1 cut(s) 1467
Psp124BI GAGCTC 1 cut(s) 711
Psp6I CCWGG 5 cut(s) 197, 733, 905, 1184, 1230
PspFI CCCAGC 1 cut(s) 1414
PspGI CCWGG 5 cut(s) 197, 733, 905, 1184, 1230
PspN4I GGNNCC 6 cut(s) 203, 304, 341, 365, 1055, 1290
PspPI GGNCC 3 cut(s) 34, 201, 369
PstI CTGCAG 2 cut(s) 832, 1029
PsuI RGATCY 2 cut(s) 832, 1012
PsyI GACNNNGTC 1 cut(s) 490
PteI GCGCGC 1 cut(s) 386
PvuI CGATCG 2 cut(s) 412, 511
RsaI GTAC 6 cut(s) 347, 437, 500, 563, 659, 1079
RsaNI GTAC 6 cut(s) 346, 436, 499, 562, 658, 1078
RseI CAYNNNNRTG 3 cut(s) 669, 822, 983
Rsr2I CGGWCCG 1 cut(s) 369
RsrII CGGWCCG 1 cut(s) 369
SacI GAGCTC 1 cut(s) 711
SacII CCGCGG 1 cut(s) 358
SalI GTCGAC 1 cut(s) 470
SaqAI TTAA 3 cut(s) 722, 1377, 1467
Sau96I GGNCC 3 cut(s) 34, 201, 369
SchI GAGTC 1 cut(s) 635
ScrFI CCNGG 6 cut(s) 199, 337, 735, 907, 1186, 1232
SduI GDGCHC 2 cut(s) 366, 711
SfaNI GCATC 3 cut(s) 814, 1099, 1176
SfcI CTRYAG 2 cut(s) 828, 1025
SfoI GGCGCC 1 cut(s) 304
Sfr303I CCGCGG 1 cut(s) 358
SgrBI CCGCGG 1 cut(s) 358
SinI GGWCC 2 cut(s) 201, 369
SmiMI CAYNNNNRTG 3 cut(s) 669, 822, 983
SmlI CTYRAG 3 cut(s) 29, 861, 1385
SmoI CTYRAG 3 cut(s) 29, 861, 1385
Sse9I AATT 8 cut(s) 475, 763, 1248, 1333, 1342, 1352, 1456, 1464
SspDI GGCGCC 1 cut(s) 302
SspMI CTAG 3 cut(s) 630, 1104, 1262
SstI GAGCTC 1 cut(s) 711
StyD4I CCNGG 6 cut(s) 197, 335, 733, 905, 1184, 1230
StyI CCWWGG 2 cut(s) 1043, 1162
TaaI ACNGT 4 cut(s) 460, 925, 955, 1366
TaiI ACGT 3 cut(s) 429, 441, 1297
TaqI TCGA 5 cut(s) 267, 408, 471, 570, 639
TasI AATT 8 cut(s) 475, 763, 1248, 1333, 1342, 1352, 1456, 1464
TatI WGTACW 1 cut(s) 1077
TauI GCSGC 8 cut(s) 162, 210, 303, 308, 360, 417, 588, 591
TfiI GAWTC 4 cut(s) 594, 680, 1370, 1390
Tru1I TTAA 3 cut(s) 722, 1377, 1467
Tru9I TTAA 3 cut(s) 722, 1377, 1467
TscAI CASTG 2 cut(s) 1029, 1307
TseFI GTSAC 1 cut(s) 795
TseI GCWGC 3 cut(s) 827, 916, 1305
Tsp45I GTSAC 1 cut(s) 795
TspDTI ATGAA 6 cut(s) 731, 744, 866, 936, 1014, 1298
TspRI CASTG 2 cut(s) 1029, 1307
Tth111I GACNNNGTC 1 cut(s) 490
Van91I CCANNNNNTGG 1 cut(s) 613
VpaK11BI GGWCC 2 cut(s) 201, 369
VspI ATTAAT 1 cut(s) 1467
XapI RAATTY 2 cut(s) 1333, 1342
XceI RCATGY 4 cut(s) 550, 1084, 1116, 1330
XcmI CCANNNNNNNNNTGG 1 cut(s) 1192
XmiI GTMKAC 1 cut(s) 471
XmnI GAANNNNTTC 3 cut(s) 679, 1005, 1352
XspI CTAG 3 cut(s) 630, 1104, 1262
ZraI GACGTC 1 cut(s) 427
Zsp2I ATGCAT 1 cut(s) 1114
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.