FvH4_2g13532

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000142
Physical Location & Seq
Forward (+)
11836955 .. 11837747
793 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g13532.t1

Sequence Viewer

Length: 585 bp
ATGCAGCACAAAAGGCAAAAACAATATCATCATAATCGTGAAGAAGATGAGACCCAGCAGGGGAGCAAGAGCAGAAATCGCATACCAAGTAGGAAGTGGTCGGAGAAACTCCCGCAAGATATCATGCAATCGATTCTACAGCGATTATGCACATCAGACTACCTTCGGTGTCGTGCAGTATGTCATTCTTGGCGATCTGCAGCTCTTGACTTAGCAATGTCGGCGCGCAAACTTGGTTGTCATCTGGCTCCTCAACTACCATGGCTTCTGTTCCCTACTCGTCGTGCCAGAGTCATGCTTCCATCAGAAACCACCATTCCAGATCACCGTCGTTCCTTGTTGAACAAAGTTGTAGCCTCTTCAGTAACGACAAGCCCGAATTGCCTTGTCGCTTTTCATTGTTACGGTTGTGGAAGGTTGGCTTTCTGTAAACCCACAGACAAATCATGGACCCTCATTGAGGTCGAGGCCGAGGCCGATCAGCTAGACGTCGATTATGTTCATGATGTAGAATTAATCGACAGGAAAATATATGCCCTCACTAAGAACGCATCAGAATTTTTGTTTGTGTTTGCATCGAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295

Protein Analysis

195

Amino Acids

22.39

Weight (kDa)

9.07

Isoelectric Point (pI)

62.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 36 - 70 1.8e-09 F-box-like
F-box PF00646 36 - 70 2.2e-09 F-box domain
Beta-prop_KIB1-4 PF03478 52 - 186 6e-14 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000142)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g13532 FvH4_2g28870 FvH4_3g30930 FvH4_3g37281 FvH4_3g37300 FvH4_3g37301 FvH4_3g37301 FvH4_4g23711 FvH4_5g02330 FvH4_6g16960 FvH4_6g16971 FvH4_6g21291 FvH4_6g21331 FvH4_6g22212 FvH4_6g22332 FvH4_6g36920 FvH4_6g37552 FvH4_6g37591 FvH4_6g39721 FvH4_6g46020 FvH4_6g47001 FvH4_6g47002 FvH4_6g47130 FvH4_6g47140 FvH4_6g47161 FvH4_6g47170 FvH4_6g47190 FvH4_6g49120 FvH4_6g49280 FvH4_6g49281 FvH4_6g49640 FvH4_6g49640
malus_domestica MD03G1085600.v1.1 MD09G1027500.v1.1 MD09G1050100.v1.1
prunus_persica Prupe.1G004300_v2.0.a1 Prupe.1G016300_v2.0.a1 Prupe.1G019300_v2.0.a1 Prupe.3G239100_v2.0.a1 Prupe.3G291200_v2.0.a1
pyrus_communis pycom08g02840 pycom08g02860 pycom08g02870
rosa_chinensis RchiOBHm_Chr2g0153831 RchiOBHm_Chr2g0166171 RchiOBHm_Chr2g0166201 RchiOBHm_Chr3g0476221 RchiOBHm_Chr4g0430971 RchiOBHm_Chr5g0067081 RchiOBHm_Chr7g0197821
rosa_laevigata RLG00000001284 RLG00000006996 RLG00000012710 RLG00000019299 RLG00000020285 RLG00000020444 RLG00000020466 RLG00000020591 RLG00000020665 RLG00000020730 RLG00000020732 RLG00000021449 RLG00000021592 RLG00000021593 RLG00000021594 RLG00000021596 RLG00000021597 RLG00000021801 RLG00000023534 RLG00000023538 RLG00000023693 RLG00000023694 RLG00000023699 RLG00000024196 RLG00000024214 RLG00000024220 RLG00000024244 RLG00000024608 RLG00000024612 RLG00000035157 RLG00000035906
rosa_multiflora Rmu_co8282815.1_g000001 Rmu_co8380495.1_g000001 Rmu_co8381111.1_g000001 Rmu_co8460513.1_g000001 Rmu_co8481947.1_g000001 Rmu_co8487937.1_g000001 Rmu_sc0000151.1_g000017 Rmu_sc0001427.1_g000003 Rmu_sc0001427.1_g000006 Rmu_sc0002611.1_g000008 Rmu_sc0003887.1_g000018 Rmu_sc0003887.1_g000019 Rmu_sc0003887.1_g000021 Rmu_sc0004119.1_g000004 Rmu_sc0004329.1_g000003 Rmu_sc0004485.1_g000024 Rmu_sc0005290.1_g000001 Rmu_sc0005609.1_g000006 Rmu_sc0006187.1_g000018 Rmu_sc0006218.1_g000032 Rmu_sc0006218.1_g000033 Rmu_sc0007150.1_g000003 Rmu_sc0007154.1_g000004 Rmu_sc0007154.1_g000005 Rmu_sc0007154.1_g000007 Rmu_sc0008314.1_g000012 Rmu_sc0010073.1_g000023 Rmu_sc0010073.1_g000025 Rmu_sc0011216.1_g000008 Rmu_sc0012445.1_g000005 Rmu_sc0013571.1_g000002 Rmu_sc0018648.1_g000002 Rmu_sc0020961.1_g000002 Rmu_sc0020961.1_g000003 Rmu_sc0023710.1_g000001 Rmu_sc0031310.1_g000008 Rmu_sc0033940.1_g000001 Rmu_sc0035493.1_g000001 Rmu_sc0039285.1_g000001
rosa_roxburghii Rroxscaffold_1G00014000 Rroxscaffold_1G00014020 Rroxscaffold_2G00082760 Rroxscaffold_2G00085090 Rroxscaffold_2G00085100 Rroxscaffold_2G00085140 Rroxscaffold_2G00086670 Rroxscaffold_2G00086680 Rroxscaffold_2G00098190 Rroxscaffold_2G00099270 Rroxscaffold_3G00227460 Rroxscaffold_3G00258190 Rroxscaffold_3G00259780 Rroxscaffold_6G00402940 Rroxscaffold_6G00410200 Rroxscaffold_6G00410210 Rroxscaffold_7G00184320
rosa_rugosa Rorug02G0416100 Rorug02G0416200 Rorug02G0520900 Rorug02G0521200 Rorug02G0521300 Rorug02G0534600 Rorug03G0116300 Rorug03G0162900 Rorug03G0162900 Rorug04G0241400 Rorug05G0380900 Rorug07G0043800
rosa_samantha Rh1AG115300 Rh2BG483700 Rh2BG509900 Rh2BG515700 Rh2BG515800 Rh2BG586500 Rh2BG586600 Rh2BG586700 Rh2BG598800 Rh2BG598900 Rh2BG599100 Rh2BG618400 Rh2BG630500 Rh2CG457400 Rh2CG484900 Rh3AG165300 Rh3AG212900 Rh3AG213300 Rh3BG145800 Rh3BG146100 Rh3BG235300 Rh3BG246500 Rh3BG247000 Rh3BG247100 Rh3CG240300 Rh3CG240800 Rh3CG240900 Rh3CG259600 Rh3DG147000 Rh3DG184800 Rh3DG184900 Rh3DG185000 Rh3DG239500 Rh3DG240100 Rh3DG240200 Rh3DG255800 Rh5BG458500 Rh5CG480400 Rh7BG170400 Rh7BG407900 Rh7CG176900
rosa_wichuraiana Rw2G038990 Rw2G041550 Rw2G047660 Rw2G048940 Rw4G026370 Rw6G008730 Rw7G014600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 492
AccII CGCG 1 cut(s) 226
AciI CCGC 1 cut(s) 113
AcsI RAATTY 1 cut(s) 557
AcuI CTGAAG 1 cut(s) 345
AcyI GRCGYC 1 cut(s) 489
AfiI CCNNNNNNNGG 2 cut(s) 60, 460
AgsI TTSAA 1 cut(s) 343
AluBI AGCT 2 cut(s) 203, 484
AluI AGCT 2 cut(s) 203, 484
Alw26I GTCTC 1 cut(s) 44
AoxI GGCC 2 cut(s) 468, 474
ApeKI GCWGC 2 cut(s) 4, 200
ApoI RAATTY 1 cut(s) 557
AseI ATTAAT 1 cut(s) 515
AspLEI GCGC 2 cut(s) 226, 228
AspS9I GGNCC 1 cut(s) 450
AsuHPI GGTGA 1 cut(s) 317
AvaII GGWCC 1 cut(s) 450
BbvI GCAGC 2 cut(s) 16, 212
BccI CCATC 1 cut(s) 310
BcoDI GTCTC 1 cut(s) 44
BfaI CTAG 1 cut(s) 485
BfmI CTRYAG 2 cut(s) 137, 198
BisI GCNGC 2 cut(s) 5, 201
BlsI GCNGC 2 cut(s) 6, 202
Bme18I GGWCC 1 cut(s) 450
BmgT120I GGNCC 1 cut(s) 450
BmiI GGNNCC 2 cut(s) 249, 452
BmsI GCATC 1 cut(s) 560
Bsa29I ATCGAT 1 cut(s) 131
BsaHI GRCGYC 1 cut(s) 489
BsaI GGTCTC 1 cut(s) 44
BsaJI CCNNGG 2 cut(s) 260, 471
Bsc4I CCNNNNNNNGG 2 cut(s) 60, 460
Bse3DI GCAATG 1 cut(s) 222
BseCI ATCGAT 1 cut(s) 131
BseDI CCNNGG 2 cut(s) 260, 471
BseLI CCNNNNNNNGG 2 cut(s) 60, 460
BseMI GCAATG 1 cut(s) 222
BsePI GCGCGC 1 cut(s) 224
BseRI GAGGAG 1 cut(s) 240
BseXI GCAGC 2 cut(s) 16, 212
BseYI CCCAGC 1 cut(s) 54
BsgI GTGCAG 1 cut(s) 195
Bsh1236I CGCG 1 cut(s) 226
BshFI GGCC 2 cut(s) 470, 476
BshVI ATCGAT 1 cut(s) 131
BslI CCNNNNNNNGG 2 cut(s) 60, 460
BsmAI GTCTC 1 cut(s) 44
BsnI GGCC 2 cut(s) 470, 476
Bso31I GGTCTC 1 cut(s) 44
Bsp143I GATC 3 cut(s) 194, 322, 478
Bsp19I CCATGG 1 cut(s) 260
BspACI CCGC 1 cut(s) 113
BspANI GGCC 2 cut(s) 470, 476
BspDI ATCGAT 1 cut(s) 131
BspFNI CGCG 1 cut(s) 226
BspHI TCATGA 1 cut(s) 502
BspLI GGNNCC 2 cut(s) 249, 452
BspMAI CTGCAG 1 cut(s) 202
BspTNI GGTCTC 1 cut(s) 44
BsrDI GCAATG 1 cut(s) 222
BssECI CCNNGG 2 cut(s) 260, 471
BssHII GCGCGC 1 cut(s) 224
BssMI GATC 3 cut(s) 194, 322, 478
BssNI GRCGYC 1 cut(s) 489
BssT1I CCWWGG 1 cut(s) 260
Bst4CI ACNGT 2 cut(s) 329, 407
Bst6I CTCTTC 1 cut(s) 364
BstACI GRCGYC 1 cut(s) 489
BstC8I GCNNGC 1 cut(s) 226
BstDEI CTNAG 2 cut(s) 211, 543
BstDSI CCRYGG 1 cut(s) 260
BstENI CCTNNNNNAGG 1 cut(s) 458
BstFNI CGCG 1 cut(s) 226
BstHHI GCGC 2 cut(s) 226, 228
BstKTI GATC 3 cut(s) 197, 325, 481
BstMAI GTCTC 1 cut(s) 44
BstMBI GATC 3 cut(s) 194, 322, 478
BstMWI GCNNNNNNNGC 4 cut(s) 13, 78, 221, 381
BstSFI CTRYAG 2 cut(s) 137, 198
BstUI CGCG 1 cut(s) 226
BstV1I GCAGC 2 cut(s) 16, 212
Bsu15I ATCGAT 1 cut(s) 131
BsuRI GGCC 2 cut(s) 470, 476
BsuTUI ATCGAT 1 cut(s) 131
BtgI CCRYGG 1 cut(s) 260
Cac8I GCNNGC 1 cut(s) 226
CciI TCATGA 1 cut(s) 502
CfoI GCGC 2 cut(s) 226, 228
Cfr13I GGNCC 1 cut(s) 450
ClaI ATCGAT 1 cut(s) 131
CviAII CATG 5 cut(s) 124, 261, 295, 447, 503
CviJI RGCY 9 cut(s) 203, 248, 265, 356, 375, 422, 470, 476, 484
CviKI_1 RGCY 9 cut(s) 203, 248, 265, 356, 375, 422, 470, 476, 484
DdeI CTNAG 2 cut(s) 211, 543
DpnI GATC 3 cut(s) 196, 324, 480
DpnII GATC 3 cut(s) 194, 322, 478
Eam1104I CTCTTC 1 cut(s) 364
EarI CTCTTC 1 cut(s) 364
Eco130I CCWWGG 1 cut(s) 260
Eco31I GGTCTC 1 cut(s) 44
Eco32I GATATC 1 cut(s) 121
Eco47I GGWCC 1 cut(s) 450
Eco57I CTGAAG 1 cut(s) 345
EcoNI CCTNNNNNAGG 1 cut(s) 458
EcoRV GATATC 1 cut(s) 121
EcoT14I CCWWGG 1 cut(s) 260
ErhI CCWWGG 1 cut(s) 260
FaeI CATG 5 cut(s) 127, 264, 298, 450, 506
FalI AAGNNNNNCTT 2 cut(s) 406, 438
FatI CATG 5 cut(s) 123, 260, 294, 446, 502
FauI CCCGC 1 cut(s) 120
Fnu4HI GCNGC 2 cut(s) 5, 201
Fsp4HI GCNGC 2 cut(s) 5, 201
FspBI CTAG 1 cut(s) 485
GlaI GCGC 2 cut(s) 225, 227
GluI GCNGC 2 cut(s) 5, 201
GsaI CCCAGC 1 cut(s) 58
HaeIII GGCC 2 cut(s) 470, 476
HhaI GCGC 2 cut(s) 226, 228
Hin1I GRCGYC 1 cut(s) 489
Hin1II CATG 5 cut(s) 127, 264, 298, 450, 506
Hin6I GCGC 2 cut(s) 224, 226
HinP1I GCGC 2 cut(s) 224, 226
HinfI GANTC 2 cut(s) 133, 291
HphI GGTGA 1 cut(s) 317
Hpy166II GTNNAC 1 cut(s) 431
Hpy188I TCNGA 4 cut(s) 103, 157, 307, 556
Hpy188III TCNNGA 4 cut(s) 38, 206, 320, 503
Hpy8I GTNNAC 1 cut(s) 431
Hpy99I CGWCG 3 cut(s) 285, 333, 494
HpyAV CCTTC 2 cut(s) 173, 408
HpyCH4III ACNGT 2 cut(s) 329, 407
HpyCH4IV ACGT 1 cut(s) 489
HpyCH4V TGCA 6 cut(s) 4, 127, 150, 176, 200, 575
HpyF10VI GCNNNNNNNGC 4 cut(s) 13, 78, 221, 381
HpyF3I CTNAG 2 cut(s) 211, 543
HpySE526I ACGT 1 cut(s) 489
Hsp92I GRCGYC 1 cut(s) 489
Hsp92II CATG 5 cut(s) 127, 264, 298, 450, 506
HspAI GCGC 2 cut(s) 224, 226
Kzo9I GATC 3 cut(s) 194, 322, 478
LmnI GCTCC 2 cut(s) 63, 253
LpnPI CCDG 6 cut(s) 44, 68, 230, 301, 333, 508
Lsp1109I GCAGC 2 cut(s) 16, 212
LweI GCATC 1 cut(s) 560
MaeI CTAG 1 cut(s) 485
MaeII ACGT 1 cut(s) 489
MaeIII GTNAC 2 cut(s) 364, 401
MalI GATC 3 cut(s) 196, 324, 480
MboI GATC 3 cut(s) 194, 322, 478
MboII GAAGA 3 cut(s) 53, 56, 351
MluCI AATT 3 cut(s) 379, 512, 557
MlyI GAGTC 1 cut(s) 300
MmeI TCCRAC 1 cut(s) 81
MnlI CCTC 7 cut(s) 261, 367, 454, 460, 464, 466, 548
MseI TTAA 1 cut(s) 515
MslI CAYNNNNRTG 1 cut(s) 36
MvnI CGCG 1 cut(s) 226
MwoI GCNNNNNNNGC 4 cut(s) 13, 78, 221, 381
NcoI CCATGG 1 cut(s) 260
NdeII GATC 3 cut(s) 194, 322, 478
NlaIII CATG 5 cut(s) 127, 264, 298, 450, 506
NlaIV GGNNCC 2 cut(s) 249, 452
NmeAIII GCCGAG 1 cut(s) 496
PagI TCATGA 1 cut(s) 502
PauI GCGCGC 1 cut(s) 224
PcsI WCGNNNNNNNCGW 1 cut(s) 374
PfeI GAWTC 1 cut(s) 133
PkrI GCNGC 2 cut(s) 6, 202
PleI GAGTC 1 cut(s) 299
PpsI GAGTC 1 cut(s) 299
PshBI ATTAAT 1 cut(s) 515
PspFI CCCAGC 1 cut(s) 54
PspN4I GGNNCC 2 cut(s) 249, 452
PspPI GGNCC 1 cut(s) 450
PstI CTGCAG 1 cut(s) 202
PteI GCGCGC 1 cut(s) 224
RseI CAYNNNNRTG 1 cut(s) 36
SaqAI TTAA 1 cut(s) 515
SatI GCNGC 2 cut(s) 5, 201
Sau3AI GATC 3 cut(s) 194, 322, 478
Sau96I GGNCC 1 cut(s) 450
SchI GAGTC 1 cut(s) 300
SetI ASST 6 cut(s) 165, 205, 419, 465, 486, 492
SfaNI GCATC 1 cut(s) 560
SfcI CTRYAG 2 cut(s) 137, 198
SinI GGWCC 1 cut(s) 450
SmiMI CAYNNNNRTG 1 cut(s) 36
Sse9I AATT 3 cut(s) 379, 512, 557
SsiI CCGC 1 cut(s) 113
SspMI CTAG 1 cut(s) 485
StyI CCWWGG 1 cut(s) 260
TaaI ACNGT 2 cut(s) 329, 407
TaiI ACGT 1 cut(s) 492
TaqI TCGA 5 cut(s) 131, 465, 492, 519, 578
TasI AATT 3 cut(s) 379, 512, 557
TfiI GAWTC 1 cut(s) 133
Tru1I TTAA 1 cut(s) 515
Tru9I TTAA 1 cut(s) 515
TseI GCWGC 2 cut(s) 4, 200
TspDTI ATGAA 2 cut(s) 386, 491
VpaK11BI GGWCC 1 cut(s) 450
VspI ATTAAT 1 cut(s) 515
XagI CCTNNNNNAGG 1 cut(s) 458
XapI RAATTY 1 cut(s) 557
XcmI CCANNNNNNNNNTGG 1 cut(s) 93
XspI CTAG 1 cut(s) 485
ZraI GACGTC 1 cut(s) 490
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.