RLG00000023694

F-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0000142
Physical Location & Seq
Reverse (-)
27159572 .. 27160717
1146 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023694

Sequence Viewer

Length: 1146 bp
ATGGATGACTACAACAGGTGGTCCAAGCATCTTCCTGATGAATTGCTTGAGATGATTGTGAAGCGTCTAACCCTAGTCGATTCAATCAGATTCGCTGCTGTGTGTCCTTCTTGGAGAAGTGTATCGTCACAATGGTTTCAACGAGTAACCCGACTTCCATGGCTCTTGAAATCGATTAAGTTTCTAGACTCAGAATCTGTGAATTATAATATCTACAGTCCAAGTGAAGACACAGTCTACGACCTCAAGTTTCGAAGAGAAAATAATAAGTTGGATGCTTCAAGTAGTAATAAGATACGTTGCTCGGGCTCTATTAATGGCTGGTTGATAATGTCTGAATGGACGTCGAAGAAGAATTTGATTCACCCTGCTAAGACGACTTCAATCAATTATTTCTTGAACCCAATGACAGGTAATCGTATTAAGCTTCCCCAGCTGACCGGAATCATCCCATGGAGCTCCGCGGCCTCTTCAGATCCAAGATGTTCTGATTGCATTGTTGCAATCTCTCCAGATCTAGGACGTTCTGATCGCGATATTATAGTTCAAGCCGGGATGAAGTTGGTGTTTTGCAGGCCAGGTAGTCATGAATCATGCCCTTGGGAAGGGCAAAAACGTTATATAGTTTACGTTAAATTTCATGAGGATGGCAAGTTATACGTATTAGAACTGGCTGATACGCATCATTATATACTTGTTGTTCTTGTTCTTGTTTCTGTTCCTGTTTTAGATCCGTTAAATCAATGTCAAGGCAAAGGCAGGTGTGAGAAAATTATTGCTTATAGGGAAAAGACAATATTAGCTCCAATGCCATCAGGGGATTCGCCGTGCGGCATTGGGAGACCATCATTTTTCTGGTCCTCCAAAGGTGAGTTTTTACTGGTTTGGGGGCGCGATTGGCCCGGCAGGAAATTTCGTGCGTTAAAGATGGATGATAAAAGTGGTTATAATCCTGTTTGGGTGGACTTGGAGACGAATGATGTTGCTGAAGACGAAGTAGTCTTAGTATCCGACTCACACACGACACACAGCACCTCAATGTCAAAGTTGAAGACATTTATGACAACCATAGACGAGGCTTTTCTTGGACTTTGTTGCCTAAAATTCTGCACCTGCCATGGTTCGTTGAATTGTGAGAATCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box F-box-like

Protein Analysis

382

Amino Acids

43.32

Weight (kDa)

8.13

Isoelectric Point (pI)

37.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 10 - 42 1.3e-07 F-box domain
Beta-prop_KIB1-4 PF03478 72 - 349 2.6e-16 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000142)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g13532 FvH4_2g28870 FvH4_3g30930 FvH4_3g37281 FvH4_3g37300 FvH4_3g37301 FvH4_3g37301 FvH4_4g23711 FvH4_5g02330 FvH4_6g16960 FvH4_6g16971 FvH4_6g21291 FvH4_6g21331 FvH4_6g22212 FvH4_6g22332 FvH4_6g36920 FvH4_6g37552 FvH4_6g37591 FvH4_6g39721 FvH4_6g46020 FvH4_6g47001 FvH4_6g47002 FvH4_6g47130 FvH4_6g47140 FvH4_6g47161 FvH4_6g47170 FvH4_6g47190 FvH4_6g49120 FvH4_6g49280 FvH4_6g49281 FvH4_6g49640 FvH4_6g49640
malus_domestica MD03G1085600.v1.1 MD09G1027500.v1.1 MD09G1050100.v1.1
prunus_persica Prupe.1G004300_v2.0.a1 Prupe.1G016300_v2.0.a1 Prupe.1G019300_v2.0.a1 Prupe.3G239100_v2.0.a1 Prupe.3G291200_v2.0.a1
pyrus_communis pycom08g02840 pycom08g02860 pycom08g02870
rosa_chinensis RchiOBHm_Chr2g0153831 RchiOBHm_Chr2g0166171 RchiOBHm_Chr2g0166201 RchiOBHm_Chr3g0476221 RchiOBHm_Chr4g0430971 RchiOBHm_Chr5g0067081 RchiOBHm_Chr7g0197821
rosa_laevigata RLG00000001284 RLG00000006996 RLG00000012710 RLG00000019299 RLG00000020285 RLG00000020444 RLG00000020466 RLG00000020591 RLG00000020665 RLG00000020730 RLG00000020732 RLG00000021449 RLG00000021592 RLG00000021593 RLG00000021594 RLG00000021596 RLG00000021597 RLG00000021801 RLG00000023534 RLG00000023538 RLG00000023693 RLG00000023694 RLG00000023699 RLG00000024196 RLG00000024214 RLG00000024220 RLG00000024244 RLG00000024608 RLG00000024612 RLG00000035157 RLG00000035906
rosa_multiflora Rmu_co8282815.1_g000001 Rmu_co8380495.1_g000001 Rmu_co8381111.1_g000001 Rmu_co8460513.1_g000001 Rmu_co8481947.1_g000001 Rmu_co8487937.1_g000001 Rmu_sc0000151.1_g000017 Rmu_sc0001427.1_g000003 Rmu_sc0001427.1_g000006 Rmu_sc0002611.1_g000008 Rmu_sc0003887.1_g000018 Rmu_sc0003887.1_g000019 Rmu_sc0003887.1_g000021 Rmu_sc0004119.1_g000004 Rmu_sc0004329.1_g000003 Rmu_sc0004485.1_g000024 Rmu_sc0005290.1_g000001 Rmu_sc0005609.1_g000006 Rmu_sc0006187.1_g000018 Rmu_sc0006218.1_g000032 Rmu_sc0006218.1_g000033 Rmu_sc0007150.1_g000003 Rmu_sc0007154.1_g000004 Rmu_sc0007154.1_g000005 Rmu_sc0007154.1_g000007 Rmu_sc0008314.1_g000012 Rmu_sc0010073.1_g000023 Rmu_sc0010073.1_g000025 Rmu_sc0011216.1_g000008 Rmu_sc0012445.1_g000005 Rmu_sc0013571.1_g000002 Rmu_sc0018648.1_g000002 Rmu_sc0020961.1_g000002 Rmu_sc0020961.1_g000003 Rmu_sc0023710.1_g000001 Rmu_sc0031310.1_g000008 Rmu_sc0033940.1_g000001 Rmu_sc0035493.1_g000001 Rmu_sc0039285.1_g000001
rosa_roxburghii Rroxscaffold_1G00014000 Rroxscaffold_1G00014020 Rroxscaffold_2G00082760 Rroxscaffold_2G00085090 Rroxscaffold_2G00085100 Rroxscaffold_2G00085140 Rroxscaffold_2G00086670 Rroxscaffold_2G00086680 Rroxscaffold_2G00098190 Rroxscaffold_2G00099270 Rroxscaffold_3G00227460 Rroxscaffold_3G00258190 Rroxscaffold_3G00259780 Rroxscaffold_6G00402940 Rroxscaffold_6G00410200 Rroxscaffold_6G00410210 Rroxscaffold_7G00184320
rosa_rugosa Rorug02G0416100 Rorug02G0416200 Rorug02G0520900 Rorug02G0521200 Rorug02G0521300 Rorug02G0534600 Rorug03G0116300 Rorug03G0162900 Rorug03G0162900 Rorug04G0241400 Rorug05G0380900 Rorug07G0043800
rosa_samantha Rh1AG115300 Rh2BG483700 Rh2BG509900 Rh2BG515700 Rh2BG515800 Rh2BG586500 Rh2BG586600 Rh2BG586700 Rh2BG598800 Rh2BG598900 Rh2BG599100 Rh2BG618400 Rh2BG630500 Rh2CG457400 Rh2CG484900 Rh3AG165300 Rh3AG212900 Rh3AG213300 Rh3BG145800 Rh3BG146100 Rh3BG235300 Rh3BG246500 Rh3BG247000 Rh3BG247100 Rh3CG240300 Rh3CG240800 Rh3CG240900 Rh3CG259600 Rh3DG147000 Rh3DG184800 Rh3DG184900 Rh3DG185000 Rh3DG239500 Rh3DG240100 Rh3DG240200 Rh3DG255800 Rh5BG458500 Rh5CG480400 Rh7BG170400 Rh7BG407900 Rh7CG176900
rosa_wichuraiana Rw2G038990 Rw2G041550 Rw2G047660 Rw2G048940 Rw4G026370 Rw6G008730 Rw7G014600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 207, 948
AarI CACCTGC 2 cut(s) 750, 1121
AasI GACNNNNNNGTC 1 cut(s) 998
AatII GACGTC 1 cut(s) 347
Acc36I ACCTGC 2 cut(s) 750, 1121
AccI GTMKAC 1 cut(s) 237
AccII CGCG 3 cut(s) 464, 534, 894
AciI CCGC 3 cut(s) 462, 464, 831
AclI AACGTT 1 cut(s) 616
AclWI GGATC 2 cut(s) 470, 725
AcsI RAATTY 4 cut(s) 355, 635, 911, 1103
AcuI CTGAAG 2 cut(s) 456, 1008
AcyI GRCGYC 1 cut(s) 344
AfiI CCNNNNNNNGG 3 cut(s) 410, 518, 605
AgsI TTSAA 9 cut(s) 84, 140, 169, 282, 384, 400, 548, 1051, 1129
AjnI CCWGG 1 cut(s) 577
AluBI AGCT 4 cut(s) 427, 436, 459, 803
AluI AGCT 4 cut(s) 427, 436, 459, 803
Alw21I GWGCWC 1 cut(s) 461
Alw26I GTCTC 2 cut(s) 835, 965
AlwI GGATC 2 cut(s) 470, 725
AlwNI CAGNNNCTG 1 cut(s) 197
Ama87I CYCGRG 1 cut(s) 304
AoxI GGCC 3 cut(s) 465, 575, 899
ApeKI GCWGC 1 cut(s) 95
ApoI RAATTY 4 cut(s) 355, 635, 911, 1103
AseI ATTAAT 1 cut(s) 315
AspLEI GCGC 1 cut(s) 894
AspS9I GGNCC 3 cut(s) 21, 858, 900
AsuC2I CCSGG 2 cut(s) 553, 903
AsuHPI GGTGA 2 cut(s) 356, 881
AsuII TTCGAA 1 cut(s) 253
AvaI CYCGRG 1 cut(s) 304
AvaII GGWCC 2 cut(s) 21, 858
BanII GRGCYC 2 cut(s) 311, 461
BarI GAAGNNNNNNTAC 2 cut(s) 138, 170
BbsI GAAGAC 3 cut(s) 234, 996, 1058
Bbv12I GWGCWC 1 cut(s) 461
BbvI GCAGC 1 cut(s) 82
BccI CCATC 4 cut(s) 641, 820, 853, 922
BceAI ACGGC 1 cut(s) 811
BciT130I CCWGG 1 cut(s) 579
BciVI GTATCC 1 cut(s) 1018
BcnI CCSGG 2 cut(s) 553, 903
BcoDI GTCTC 2 cut(s) 835, 965
BfaI CTAG 3 cut(s) 74, 185, 518
BfmI CTRYAG 1 cut(s) 214
BfuAI ACCTGC 2 cut(s) 750, 1121
BfuI GTATCC 1 cut(s) 1018
BglII AGATCT 1 cut(s) 514
BisI GCNGC 3 cut(s) 96, 465, 832
BlsI GCNGC 3 cut(s) 97, 466, 833
Bme1390I CCNGG 3 cut(s) 553, 579, 903
Bme18I GGWCC 2 cut(s) 21, 858
BmeT110I CYCGRG 1 cut(s) 304
BmgT120I GGNCC 3 cut(s) 21, 858, 900
BmrFI CCNGG 3 cut(s) 553, 579, 903
BmsI GCATC 3 cut(s) 37, 265, 691
BpiI GAAGAC 3 cut(s) 234, 996, 1058
BpmI CTGGAG 1 cut(s) 495
Bpu14I TTCGAA 1 cut(s) 253
BpuEI CTTGAG 2 cut(s) 68, 230
BpuMI CCSGG 2 cut(s) 553, 903
Bsa29I ATCGAT 1 cut(s) 173
BsaAI YACGTR 1 cut(s) 661
BsaBI GATNNNNATC 1 cut(s) 681
BsaHI GRCGYC 1 cut(s) 344
BsaI GGTCTC 1 cut(s) 835
BsaJI CCNNGG 5 cut(s) 158, 452, 462, 599, 1117
BsaWI WCCGGW 1 cut(s) 440
Bsc4I CCNNNNNNNGG 3 cut(s) 410, 518, 605
Bse1I ACTGG 2 cut(s) 675, 885
Bse8I GATNNNNATC 1 cut(s) 681
BseBI CCWGG 1 cut(s) 579
BseCI ATCGAT 1 cut(s) 173
BseDI CCNNGG 5 cut(s) 158, 452, 462, 599, 1117
BseGI GGATG 6 cut(s) 10, 280, 447, 561, 652, 937
BseJI GATNNNNATC 1 cut(s) 681
BseLI CCNNNNNNNGG 3 cut(s) 410, 518, 605
BseMII CTCAG 1 cut(s) 204
BseNI ACTGG 2 cut(s) 675, 885
BseXI GCAGC 1 cut(s) 82
BseYI CCCAGC 1 cut(s) 432
BsgI GTGCAG 1 cut(s) 1093
Bsh1236I CGCG 3 cut(s) 464, 534, 894
BshFI GGCC 3 cut(s) 467, 577, 901
BshVI ATCGAT 1 cut(s) 173
BsiHKAI GWGCWC 1 cut(s) 461
BsiHKCI CYCGRG 1 cut(s) 304
BsiSI CCGG 3 cut(s) 441, 552, 903
BslI CCNNNNNNNGG 3 cut(s) 410, 518, 605
BsmAI GTCTC 2 cut(s) 835, 965
BsmBI CGTCTC 1 cut(s) 965
BsnI GGCC 3 cut(s) 467, 577, 901
Bso31I GGTCTC 1 cut(s) 835
BsoBI CYCGRG 1 cut(s) 304
Bsp119I TTCGAA 1 cut(s) 253
Bsp1286I GDGCHC 2 cut(s) 311, 461
Bsp143I GATC 4 cut(s) 475, 514, 529, 730
Bsp19I CCATGG 3 cut(s) 158, 452, 1117
Bsp68I TCGCGA 1 cut(s) 534
BspACI CCGC 3 cut(s) 462, 464, 831
BspANI GGCC 3 cut(s) 467, 577, 901
BspCNI CTCAG 1 cut(s) 203
BspDI ATCGAT 1 cut(s) 173
BspFNI CGCG 3 cut(s) 464, 534, 894
BspHI TCATGA 2 cut(s) 586, 640
BspMI ACCTGC 2 cut(s) 750, 1121
BspPI GGATC 2 cut(s) 470, 725
BspT104I TTCGAA 1 cut(s) 253
BspTNI GGTCTC 1 cut(s) 835
BsrI ACTGG 2 cut(s) 675, 885
BssECI CCNNGG 5 cut(s) 158, 452, 462, 599, 1117
BssMI GATC 4 cut(s) 475, 514, 529, 730
BssNI GRCGYC 1 cut(s) 344
BssT1I CCWWGG 4 cut(s) 158, 452, 599, 1117
Bst2UI CCWGG 1 cut(s) 579
Bst4CI ACNGT 2 cut(s) 218, 235
Bst6I CTCTTC 2 cut(s) 250, 475
BstACI GRCGYC 1 cut(s) 344
BstBAI YACGTR 1 cut(s) 661
BstBI TTCGAA 1 cut(s) 253
BstC8I GCNNGC 1 cut(s) 575
BstDEI CTNAG 3 cut(s) 190, 372, 1003
BstDSI CCRYGG 4 cut(s) 158, 452, 462, 1117
BstENI CCTNNNNNAGG 1 cut(s) 603
BstF5I GGATG 6 cut(s) 10, 280, 447, 561, 652, 937
BstFNI CGCG 3 cut(s) 464, 534, 894
BstHHI GCGC 1 cut(s) 894
BstKTI GATC 4 cut(s) 478, 517, 532, 733
BstMAI GTCTC 2 cut(s) 835, 965
BstMBI GATC 4 cut(s) 475, 514, 529, 730
BstMWI GCNNNNNNNGC 2 cut(s) 433, 898
BstNI CCWGG 1 cut(s) 579
BstSCI CCNGG 3 cut(s) 551, 577, 901
BstSFI CTRYAG 1 cut(s) 214
BstSNI TACGTA 1 cut(s) 661
BstUI CGCG 3 cut(s) 464, 534, 894
BstV1I GCAGC 1 cut(s) 82
BstV2I GAAGAC 3 cut(s) 234, 996, 1058
BstX2I RGATCY 3 cut(s) 475, 514, 730
BstYI RGATCY 3 cut(s) 475, 514, 730
Bsu15I ATCGAT 1 cut(s) 173
BsuI GTATCC 1 cut(s) 1018
BsuRI GGCC 3 cut(s) 467, 577, 901
BsuTUI ATCGAT 1 cut(s) 173
BtgI CCRYGG 4 cut(s) 158, 452, 462, 1117
BtsCI GGATG 6 cut(s) 10, 280, 447, 561, 652, 937
BtuMI TCGCGA 1 cut(s) 534
BveI ACCTGC 2 cut(s) 750, 1121
Cac8I GCNNGC 1 cut(s) 575
CaiI CAGNNNCTG 1 cut(s) 197
CciI TCATGA 2 cut(s) 586, 640
CfoI GCGC 1 cut(s) 894
Cfr13I GGNCC 3 cut(s) 21, 858, 900
Cfr42I CCGCGG 1 cut(s) 465
ClaI ATCGAT 1 cut(s) 173
CseI GACGC 1 cut(s) 53
CviAII CATG 6 cut(s) 159, 453, 587, 594, 641, 1118
DdeI CTNAG 3 cut(s) 190, 372, 1003
DpnI GATC 4 cut(s) 477, 516, 531, 732
DpnII GATC 4 cut(s) 475, 514, 529, 730
DrdI GACNNNNNNGTC 1 cut(s) 998
DseDI GACNNNNNNGTC 1 cut(s) 998
Eam1104I CTCTTC 2 cut(s) 250, 475
EarI CTCTTC 2 cut(s) 250, 475
Ecl136II GAGCTC 1 cut(s) 459
Eco105I TACGTA 1 cut(s) 661
Eco130I CCWWGG 4 cut(s) 158, 452, 599, 1117
Eco24I GRGCYC 2 cut(s) 311, 461
Eco31I GGTCTC 1 cut(s) 835
Eco47I GGWCC 2 cut(s) 21, 858
Eco53kI GAGCTC 1 cut(s) 459
Eco57I CTGAAG 2 cut(s) 456, 1008
Eco88I CYCGRG 1 cut(s) 304
EcoICRI GAGCTC 1 cut(s) 459
EcoNI CCTNNNNNAGG 1 cut(s) 603
EcoRII CCWGG 1 cut(s) 577
EcoT14I CCWWGG 4 cut(s) 158, 452, 599, 1117
EcoT38I GRGCYC 2 cut(s) 311, 461
ErhI CCWWGG 4 cut(s) 158, 452, 599, 1117
Esp3I CGTCTC 1 cut(s) 965
FaeI CATG 6 cut(s) 162, 456, 590, 597, 644, 1121
FatI CATG 6 cut(s) 158, 452, 586, 593, 640, 1117
FblI GTMKAC 1 cut(s) 237
Fnu4HI GCNGC 3 cut(s) 96, 465, 832
FokI GGATG 6 cut(s) 17, 287, 434, 568, 659, 944
FriOI GRGCYC 2 cut(s) 311, 461
Fsp4HI GCNGC 3 cut(s) 96, 465, 832
FspBI CTAG 3 cut(s) 74, 185, 518
GlaI GCGC 1 cut(s) 893
GluI GCNGC 3 cut(s) 96, 465, 832
GsaI CCCAGC 1 cut(s) 436
GsuI CTGGAG 1 cut(s) 495
HaeIII GGCC 3 cut(s) 467, 577, 901
HapII CCGG 3 cut(s) 441, 552, 903
HgaI GACGC 1 cut(s) 53
HhaI GCGC 1 cut(s) 894
Hin1I GRCGYC 1 cut(s) 344
Hin1II CATG 6 cut(s) 162, 456, 590, 597, 644, 1121
Hin6I GCGC 1 cut(s) 892
HinP1I GCGC 1 cut(s) 892
HindIII AAGCTT 1 cut(s) 425
HpaII CCGG 3 cut(s) 441, 552, 903
HphI GGTGA 2 cut(s) 356, 881
Hpy166II GTNNAC 3 cut(s) 238, 628, 964
Hpy188I TCNGA 7 cut(s) 89, 193, 337, 475, 490, 529, 1012
Hpy188III TCNNGA 8 cut(s) 35, 166, 185, 397, 512, 533, 587, 641
Hpy8I GTNNAC 3 cut(s) 238, 628, 964
Hpy99I CGWCG 1 cut(s) 349
HpyAV CCTTC 2 cut(s) 117, 599
HpyCH4III ACNGT 2 cut(s) 218, 235
HpyCH4IV ACGT 6 cut(s) 298, 344, 523, 616, 630, 660
HpyCH4V TGCA 4 cut(s) 495, 503, 573, 1110
HpyF10VI GCNNNNNNNGC 2 cut(s) 433, 898
HpyF3I CTNAG 3 cut(s) 190, 372, 1003
HpySE526I ACGT 6 cut(s) 298, 344, 523, 616, 630, 660
Hsp92I GRCGYC 1 cut(s) 344
Hsp92II CATG 6 cut(s) 162, 456, 590, 597, 644, 1121
HspAI GCGC 1 cut(s) 892
KspI CCGCGG 1 cut(s) 465
Kzo9I GATC 4 cut(s) 475, 514, 529, 730
LmnI GCTCC 3 cut(s) 456, 464, 808
Lsp1109I GCAGC 1 cut(s) 82
LweI GCATC 3 cut(s) 37, 265, 691
MaeI CTAG 3 cut(s) 74, 185, 518
MaeII ACGT 6 cut(s) 298, 344, 523, 616, 630, 660
MaeIII GTNAC 2 cut(s) 126, 145
MalI GATC 4 cut(s) 477, 516, 531, 732
MboI GATC 4 cut(s) 475, 514, 529, 730
MboII GAAGA 8 cut(s) 23, 239, 267, 361, 364, 462, 1001, 1063
MflI RGATCY 3 cut(s) 475, 514, 730
MhlI GDGCHC 2 cut(s) 311, 461
MluCI AATT 9 cut(s) 41, 202, 355, 388, 635, 771, 911, 1103, 1129
MlyI GAGTC 2 cut(s) 182, 1007
MmeI TCCRAC 2 cut(s) 252, 1035
MnlI CCTC 6 cut(s) 254, 478, 637, 871, 1045, 1069
MseI TTAA 6 cut(s) 177, 315, 423, 633, 737, 923
MslI CAYNNNNRTG 2 cut(s) 645, 1037
MspA1I CMGCKG 2 cut(s) 436, 464
MspI CCGG 3 cut(s) 441, 552, 903
MspR9I CCNGG 3 cut(s) 553, 579, 903
MvaI CCWGG 1 cut(s) 579
MvnI CGCG 3 cut(s) 464, 534, 894
MwoI GCNNNNNNNGC 2 cut(s) 433, 898
NciI CCSGG 2 cut(s) 553, 903
NcoI CCATGG 3 cut(s) 158, 452, 1117
NdeII GATC 4 cut(s) 475, 514, 529, 730
NlaIII CATG 6 cut(s) 162, 456, 590, 597, 644, 1121
NmuCI GTSAC 1 cut(s) 126
NruI TCGCGA 1 cut(s) 534
NspV TTCGAA 1 cut(s) 253
PagI TCATGA 2 cut(s) 586, 640
PaqCI CACCTGC 2 cut(s) 750, 1121
PcsI WCGNNNNNNNCGW 1 cut(s) 148
PfeI GAWTC 8 cut(s) 80, 90, 194, 361, 444, 590, 821, 1138
PflFI GACNNNGTC 1 cut(s) 233
PkrI GCNGC 3 cut(s) 97, 466, 833
PleI GAGTC 2 cut(s) 182, 1007
PpsI GAGTC 2 cut(s) 182, 1007
Ppu21I YACGTR 1 cut(s) 661
PshBI ATTAAT 1 cut(s) 315
PsiI TTATAA 2 cut(s) 207, 948
Psp124BI GAGCTC 1 cut(s) 461
Psp1406I AACGTT 1 cut(s) 616
Psp6I CCWGG 1 cut(s) 577
PspFI CCCAGC 1 cut(s) 432
PspGI CCWGG 1 cut(s) 577
PspPI GGNCC 3 cut(s) 21, 858, 900
PstNI CAGNNNCTG 1 cut(s) 197
PsuI RGATCY 3 cut(s) 475, 514, 730
PsyI GACNNNGTC 1 cut(s) 233
PvuII CAGCTG 1 cut(s) 436
RruI TCGCGA 1 cut(s) 534
RseI CAYNNNNRTG 2 cut(s) 645, 1037
SacI GAGCTC 1 cut(s) 461
SacII CCGCGG 1 cut(s) 465
SaqAI TTAA 6 cut(s) 177, 315, 423, 633, 737, 923
SatI GCNGC 3 cut(s) 96, 465, 832
Sau3AI GATC 4 cut(s) 475, 514, 529, 730
Sau96I GGNCC 3 cut(s) 21, 858, 900
SchI GAGTC 2 cut(s) 182, 1007
ScrFI CCNGG 3 cut(s) 553, 579, 903
SduI GDGCHC 2 cut(s) 311, 461
SfaNI GCATC 3 cut(s) 37, 265, 691
SfcI CTRYAG 1 cut(s) 214
Sfr303I CCGCGG 1 cut(s) 465
SfuI TTCGAA 1 cut(s) 253
SgrBI CCGCGG 1 cut(s) 465
SinI GGWCC 2 cut(s) 21, 858
SmiMI CAYNNNNRTG 2 cut(s) 645, 1037
SmlI CTYRAG 2 cut(s) 47, 245
SmoI CTYRAG 2 cut(s) 47, 245
SnaBI TACGTA 1 cut(s) 661
Sse9I AATT 9 cut(s) 41, 202, 355, 388, 635, 771, 911, 1103, 1129
SsiI CCGC 3 cut(s) 462, 464, 831
SspI AATATT 1 cut(s) 798
SspMI CTAG 3 cut(s) 74, 185, 518
SstI GAGCTC 1 cut(s) 461
StyD4I CCNGG 3 cut(s) 551, 577, 901
StyI CCWWGG 4 cut(s) 158, 452, 599, 1117
TaaI ACNGT 2 cut(s) 218, 235
TaiI ACGT 6 cut(s) 301, 347, 526, 619, 633, 663
TaqI TCGA 4 cut(s) 78, 173, 253, 347
TasI AATT 9 cut(s) 41, 202, 355, 388, 635, 771, 911, 1103, 1129
TauI GCSGC 2 cut(s) 467, 834
TfiI GAWTC 8 cut(s) 80, 90, 194, 361, 444, 590, 821, 1138
Tru1I TTAA 6 cut(s) 177, 315, 423, 633, 737, 923
Tru9I TTAA 6 cut(s) 177, 315, 423, 633, 737, 923
TseFI GTSAC 1 cut(s) 126
TseI GCWGC 1 cut(s) 95
Tsp45I GTSAC 1 cut(s) 126
TspDTI ATGAA 4 cut(s) 54, 572, 603, 629
TspGWI ACGGA 1 cut(s) 723
Tth111I GACNNNGTC 1 cut(s) 233
VpaK11BI GGWCC 2 cut(s) 21, 858
VspI ATTAAT 1 cut(s) 315
XagI CCTNNNNNAGG 1 cut(s) 603
XapI RAATTY 4 cut(s) 355, 635, 911, 1103
XbaI TCTAGA 1 cut(s) 184
XcmI CCANNNNNNNNNTGG 1 cut(s) 852
XmiI GTMKAC 1 cut(s) 237
XspI CTAG 3 cut(s) 74, 185, 518
ZraI GACGTC 1 cut(s) 345
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.