Rh3DG239500

Tropinone reductase homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0000142
Physical Location & Seq
Reverse (-)
23040297 .. 23062852
22556 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG239500.1

Sequence Viewer

Length: 2166 bp
ATGGATGACCACAACAGGTGGTCCAAGCATCTTCCTGATGAATTGATTGAAATGATTGTGAAGCGTCTAACCCTAGTCGATTCAATCAGATTCGCTGCTGTGTGTCCTTCTTGGAGAAGTGTATCATCACAATGGTCTCAACGAGTGACCCGACTTCCATGGCTCTTGGATTCGACTGAGATTCTAGACTCAGAAACTGTGAATTATAGGCTCTACAGTCCAAGTGAAGACAGAGTCTACGACCTCAAGTTTCCAAGAGAAAATAATAAGTCGGATGCTTCAGATAGTTATGGGATACGTTGCTTGGGCTCTATTAATGGCTGGTTGATAATGTCTGAATGGACATTGAATAATATTTCGTATGATGATAAGTCCATCAATTATTTCTTGAACCCAATGACAGGTGATCGTATTAAGCTGCCCCAGCAGACCGAATTCATCCCAAAGACATCCGCAGCCTCTTCAGATCCAAGATGTTCTGATTGCATTGTTGCAATTGGAGAAGGGTCGAATTTGGTATTTTGCAGGCCAAGTAGTCATGAATTATGCCCTTGGGAAGGGCACCAAGATTGTCATGTACAAAAAGTTAAATTTCATGAGGATGGCAAGTTATACGTAATAGGATTGGGTGAAACGTGCTGTATACTTGTTGTTCTTGTTCTTGTTTTAGATCCCTTAAATCAATGTCAAGGCAAAGGCAGGTGTGAGAAAATTATTGCTTATAGGGAAATGACAATATCAGTTCCAGGGGATCCGTACAACGAAACCGCGCTGTCCTGGTCTCCATCATTTTTCTGGTCCTCCAAAGGTGAGTTTTTATTGTTTTGGAGGTTTCGCAGAGGCGACTTTCCATTTCGAATGTTCAGGATAGATGATAAAAGTGGTTATAATCCTATTTGGGTGAAATTGGAGAAGAATGATATTGCTGAAGACGAAGTAGTCTTTCTATCCTACTTTCACAGCACCTCAATGTCAAAGTCGAAGTCATTTATGACAACCATAGACGAGGCTTTTCTTGGACTTCATTGCGGAAATTTTTTCATCGACGATCCCGATCGTTCGTTGACTAGTGAATTGAATATTTTAGATTGGGTATGGTACATAATTTTGGGGGAACCTTTCTTCTTGGTTATTCATGAAGGTGAAACTTTAGCTGAAGTGATAGCATGGATACAAAAGAAATTGCAGGTCCCTGATGAAGAGTTCGCAAAGTGGAAGTTTGCATTCTTGTTATTGGGTCGTCCCGAGTACCTTCAGGACTCCGATGTAGTTTCCAGTCGTATTCAGACAAGAGTTGTTTATGGTGCTTGGGAGCAGTACCTGGGGTTAGAGTATGCTGATAGTGCTCCTAAGAAGCCATGCAGTAAATCAGAAGCAACCATGTCTGGGTTCAAAAGCCAAAGGTGGTCCCTTAAGGGTATGACTGCACTCGTCACCGGTGGAACTAGAGGCATTGGATATGCCATTGTCGAAGAACTAGCAGGACTTGGGGCAACAGTGCATACCTGTGCTCGTAACAAGGAAATGATCGATGAGAGGGTTCGAGAGTGGAAGATTAAGGGATTTACCGTGAGTGGTTCAGTTTATGACCTCACATCTAAATCTCAAAGGGAGGAGCTCATAAAGACTGTCTCATCTGTTTTTGATGGCAAGCTTAGCATCCTTGTAAATAATGCTGCAATAGTCACACTCAGAGGAGCTACAGATTATAGTTTAGAAGATTTCTCAAGTATGATGAGCAGCAATGTTGAATCTCCCTACCATCTTTGTCAACTAGCACATCCTCTTCTGAAGGCATCAGGAAATGGAAGCATTATATTTGTCTCCTCTATTGCTGGTATAATAGCTCTGCCTAAACTCTCCGCCTATGCAGCAACCAAAGGAGCTATCATCCAGATTTCAAAGAATTTGGCATGTGAATGGGCAAAAGACAACATTCGAACAAATACTGTAGCACCATGGGCAGTCAACACTGGGGCAGCAGCAGTTGAACCGGATGAGCATCGTGATGATTTCAGAAGGCTAATAGCTCGAACTCCCATTCGTCGCCTTGCAGACCCTAATGAAATTTCTTCTCTGGTGACTTTTCTTTGCCTCCCAGCTGCTTCTTACATCAATGGACAAGTTGTTAGCATTGATGGAGGATTTACAGTTGGTGGTTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00960 ko01100 ko01110 map00960 map01100 map01110
Pfam Domains
Protein Families
adh_short adh_short_C2

Protein Analysis

721

Amino Acids

81.13

Weight (kDa)

5.62

Isoelectric Point (pI)

35.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 10 - 42 5.9e-07 F-box domain
Beta-prop_KIB1-4 PF03478 71 - 328 2.6e-19 KIB1-4 beta-propeller
USP7_C2 PF14533 371 - 449 5.9e-13 Ubiquitin-specific protease C-terminal
adh_short PF00106 475 - 662 1.4e-45 short chain dehydrogenase
KR PF08659 475 - 627 3.4e-11 KR domain
adh_short_C2 PF13561 483 - 717 1.7e-52 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000142)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g13532 FvH4_2g28870 FvH4_3g30930 FvH4_3g37281 FvH4_3g37300 FvH4_3g37301 FvH4_3g37301 FvH4_4g23711 FvH4_5g02330 FvH4_6g16960 FvH4_6g16971 FvH4_6g21291 FvH4_6g21331 FvH4_6g22212 FvH4_6g22332 FvH4_6g36920 FvH4_6g37552 FvH4_6g37591 FvH4_6g39721 FvH4_6g46020 FvH4_6g47001 FvH4_6g47002 FvH4_6g47130 FvH4_6g47140 FvH4_6g47161 FvH4_6g47170 FvH4_6g47190 FvH4_6g49120 FvH4_6g49280 FvH4_6g49281 FvH4_6g49640 FvH4_6g49640
malus_domestica MD03G1085600.v1.1 MD09G1027500.v1.1 MD09G1050100.v1.1
prunus_persica Prupe.1G004300_v2.0.a1 Prupe.1G016300_v2.0.a1 Prupe.1G019300_v2.0.a1 Prupe.3G239100_v2.0.a1 Prupe.3G291200_v2.0.a1
pyrus_communis pycom08g02840 pycom08g02860 pycom08g02870
rosa_chinensis RchiOBHm_Chr2g0153831 RchiOBHm_Chr2g0166171 RchiOBHm_Chr2g0166201 RchiOBHm_Chr3g0476221 RchiOBHm_Chr4g0430971 RchiOBHm_Chr5g0067081 RchiOBHm_Chr7g0197821
rosa_laevigata RLG00000001284 RLG00000006996 RLG00000012710 RLG00000019299 RLG00000020285 RLG00000020444 RLG00000020466 RLG00000020591 RLG00000020665 RLG00000020730 RLG00000020732 RLG00000021449 RLG00000021592 RLG00000021593 RLG00000021594 RLG00000021596 RLG00000021597 RLG00000021801 RLG00000023534 RLG00000023538 RLG00000023693 RLG00000023694 RLG00000023699 RLG00000024196 RLG00000024214 RLG00000024220 RLG00000024244 RLG00000024608 RLG00000024612 RLG00000035157 RLG00000035906
rosa_multiflora Rmu_co8282815.1_g000001 Rmu_co8380495.1_g000001 Rmu_co8381111.1_g000001 Rmu_co8460513.1_g000001 Rmu_co8481947.1_g000001 Rmu_co8487937.1_g000001 Rmu_sc0000151.1_g000017 Rmu_sc0001427.1_g000003 Rmu_sc0001427.1_g000006 Rmu_sc0002611.1_g000008 Rmu_sc0003887.1_g000018 Rmu_sc0003887.1_g000019 Rmu_sc0003887.1_g000021 Rmu_sc0004119.1_g000004 Rmu_sc0004329.1_g000003 Rmu_sc0004485.1_g000024 Rmu_sc0005290.1_g000001 Rmu_sc0005609.1_g000006 Rmu_sc0006187.1_g000018 Rmu_sc0006218.1_g000032 Rmu_sc0006218.1_g000033 Rmu_sc0007150.1_g000003 Rmu_sc0007154.1_g000004 Rmu_sc0007154.1_g000005 Rmu_sc0007154.1_g000007 Rmu_sc0008314.1_g000012 Rmu_sc0010073.1_g000023 Rmu_sc0010073.1_g000025 Rmu_sc0011216.1_g000008 Rmu_sc0012445.1_g000005 Rmu_sc0013571.1_g000002 Rmu_sc0018648.1_g000002 Rmu_sc0020961.1_g000002 Rmu_sc0020961.1_g000003 Rmu_sc0023710.1_g000001 Rmu_sc0031310.1_g000008 Rmu_sc0033940.1_g000001 Rmu_sc0035493.1_g000001 Rmu_sc0039285.1_g000001
rosa_roxburghii Rroxscaffold_1G00014000 Rroxscaffold_1G00014020 Rroxscaffold_2G00082760 Rroxscaffold_2G00085090 Rroxscaffold_2G00085100 Rroxscaffold_2G00085140 Rroxscaffold_2G00086670 Rroxscaffold_2G00086680 Rroxscaffold_2G00098190 Rroxscaffold_2G00099270 Rroxscaffold_3G00227460 Rroxscaffold_3G00258190 Rroxscaffold_3G00259780 Rroxscaffold_6G00402940 Rroxscaffold_6G00410200 Rroxscaffold_6G00410210 Rroxscaffold_7G00184320
rosa_rugosa Rorug02G0416100 Rorug02G0416200 Rorug02G0520900 Rorug02G0521200 Rorug02G0521300 Rorug02G0534600 Rorug03G0116300 Rorug03G0162900 Rorug03G0162900 Rorug04G0241400 Rorug05G0380900 Rorug07G0043800
rosa_samantha Rh1AG115300 Rh2BG483700 Rh2BG509900 Rh2BG515700 Rh2BG515800 Rh2BG586500 Rh2BG586600 Rh2BG586700 Rh2BG598800 Rh2BG598900 Rh2BG599100 Rh2BG618400 Rh2BG630500 Rh2CG457400 Rh2CG484900 Rh3AG165300 Rh3AG212900 Rh3AG213300 Rh3BG145800 Rh3BG146100 Rh3BG235300 Rh3BG246500 Rh3BG247000 Rh3BG247100 Rh3CG240300 Rh3CG240800 Rh3CG240900 Rh3CG259600 Rh3DG147000 Rh3DG184800 Rh3DG184900 Rh3DG185000 Rh3DG239500 Rh3DG240100 Rh3DG240200 Rh3DG255800 Rh5BG458500 Rh5CG480400 Rh7BG170400 Rh7BG407900 Rh7CG176900
rosa_wichuraiana Rw2G038990 Rw2G041550 Rw2G047660 Rw2G048940 Rw4G026370 Rw6G008730 Rw7G014600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 888
AarI CACCTGC 1 cut(s) 690
AasI GACNNNNNNGTC 1 cut(s) 938
Acc36I ACCTGC 2 cut(s) 690, 1177
AccB1I GGYRCC 1 cut(s) 561
AccI GTMKAC 2 cut(s) 237, 643
AccII CGCG 1 cut(s) 770
AciI CCGC 4 cut(s) 453, 768, 1029, 1863
AclWI GGATC 5 cut(s) 461, 665, 746, 759, 1043
AcsI RAATTY 6 cut(s) 434, 511, 590, 1033, 1906, 2067
AcuI CTGAAG 6 cut(s) 264, 447, 948, 1176, 1238, 1811
AfaI GTAC 5 cut(s) 579, 758, 1100, 1250, 1319
AfiI CCNNNNNNNGG 3 cut(s) 401, 557, 1386
AflII CTTAAG 1 cut(s) 1412
AgeI ACCGGT 1 cut(s) 1436
AgsI TTSAA 9 cut(s) 50, 84, 349, 391, 1078, 1393, 1751, 1902, 1991
AhlI ACTAGT 1 cut(s) 1067
AjnI CCWGG 3 cut(s) 745, 776, 1320
AluBI AGCT 9 cut(s) 418, 1154, 1618, 1654, 1700, 1847, 1886, 2030, 2102
AluI AGCT 9 cut(s) 418, 1154, 1618, 1654, 1700, 1847, 1886, 2030, 2102
Alw21I GWGCWC 3 cut(s) 1348, 1513, 1620
Alw26I GTCTC 4 cut(s) 141, 786, 1636, 1828
AlwI GGATC 5 cut(s) 461, 665, 746, 759, 1043
AlwNI CAGNNNCTG 2 cut(s) 197, 1321
Ama87I CYCGRG 1 cut(s) 1244
AoxI GGCC 1 cut(s) 527
ApeKI GCWGC 9 cut(s) 95, 418, 455, 1676, 1740, 1871, 1979, 1982, 2102
ApoI RAATTY 6 cut(s) 434, 511, 590, 1033, 1906, 2067
ArsI GACNNNNNNTTYG 2 cut(s) 968, 1000
AseI ATTAAT 1 cut(s) 315
AsiGI ACCGGT 1 cut(s) 1436
AspLEI GCGC 1 cut(s) 772
AspS9I GGNCC 4 cut(s) 21, 798, 1189, 1407
AsuHPI GGTGA 7 cut(s) 416, 641, 821, 913, 1154, 1426, 2092
AsuII TTCGAA 2 cut(s) 856, 1939
AvaI CYCGRG 1 cut(s) 1244
AvaII GGWCC 4 cut(s) 21, 798, 1189, 1407
BaeGI GKGCMC 1 cut(s) 564
BamHI GGATCC 1 cut(s) 751
BanI GGYRCC 1 cut(s) 561
BanII GRGCYC 2 cut(s) 311, 1620
BbsI GAAGAC 2 cut(s) 234, 936
Bbv12I GWGCWC 3 cut(s) 1348, 1513, 1620
BbvI GCAGC 9 cut(s) 82, 405, 467, 1663, 1752, 1883, 1991, 1994, 2089
BccI CCATC 6 cut(s) 383, 596, 793, 1640, 1770, 2132
BciT130I CCWGG 3 cut(s) 747, 778, 1322
BciVI GTATCC 2 cut(s) 288, 1164
BcoDI GTCTC 4 cut(s) 141, 786, 1636, 1828
BcuI ACTAGT 1 cut(s) 1067
BfaI CTAG 7 cut(s) 74, 185, 1068, 1446, 1478, 1775, 2164
BfmI CTRYAG 3 cut(s) 214, 1701, 1950
BfrI CTTAAG 1 cut(s) 1412
BfuAI ACCTGC 2 cut(s) 690, 1177
BfuI GTATCC 2 cut(s) 288, 1164
BisI GCNGC 9 cut(s) 96, 419, 456, 1677, 1741, 1872, 1980, 1983, 2103
BlpI GCTNAGC 1 cut(s) 1655
BlsI GCNGC 9 cut(s) 97, 420, 457, 1678, 1742, 1873, 1981, 1984, 2104
Bme1390I CCNGG 3 cut(s) 747, 778, 1322
Bme18I GGWCC 4 cut(s) 21, 798, 1189, 1407
BmeT110I CYCGRG 1 cut(s) 1244
BmgT120I GGNCC 4 cut(s) 21, 798, 1189, 1407
BmiI GGNNCC 5 cut(s) 563, 753, 1116, 1191, 1409
BmrFI CCNGG 3 cut(s) 747, 778, 1322
BmrI ACTGGG 1 cut(s) 1983
BmsI GCATC 5 cut(s) 37, 265, 1668, 1805, 2011
BmuI ACTGGG 1 cut(s) 1983
BpiI GAAGAC 2 cut(s) 234, 936
Bpu1102I GCTNAGC 1 cut(s) 1655
Bpu14I TTCGAA 2 cut(s) 856, 1939
BpuEI CTTGAG 2 cut(s) 230, 1711
Bsa29I ATCGAT 1 cut(s) 1530
BsaAI YACGTR 1 cut(s) 616
BsaBI GATNNNNATC 2 cut(s) 1053, 2001
BsaI GGTCTC 2 cut(s) 141, 786
BsaJI CCNNGG 5 cut(s) 158, 551, 746, 1321, 1958
BsaWI WCCGGW 2 cut(s) 1436, 1993
Bsc4I CCNNNNNNNGG 3 cut(s) 401, 557, 1386
Bse118I RCCGGY 1 cut(s) 1436
Bse1I ACTGG 2 cut(s) 1275, 1978
Bse3DI GCAATG 2 cut(s) 1024, 1750
Bse8I GATNNNNATC 2 cut(s) 1053, 2001
BseBI CCWGG 3 cut(s) 747, 778, 1322
BseCI ATCGAT 1 cut(s) 1530
BseDI CCNNGG 5 cut(s) 158, 551, 746, 1321, 1958
BseGI GGATG 9 cut(s) 10, 280, 438, 449, 607, 1659, 1780, 1890, 2002
BseJI GATNNNNATC 2 cut(s) 1053, 2001
BseLI CCNNNNNNNGG 3 cut(s) 401, 557, 1386
BseMI GCAATG 2 cut(s) 1024, 1750
BseMII CTCAG 3 cut(s) 168, 204, 1705
BseNI ACTGG 2 cut(s) 1275, 1978
BseRI GAGGAG 3 cut(s) 1628, 1710, 1816
BseSI GKGCMC 1 cut(s) 564
BseXI GCAGC 9 cut(s) 82, 405, 467, 1663, 1752, 1883, 1991, 1994, 2089
BseYI CCCAGC 2 cut(s) 423, 2098
BsgI GTGCAG 1 cut(s) 1410
Bsh1236I CGCG 1 cut(s) 770
Bsh1285I CGRYCG 1 cut(s) 1057
BshFI GGCC 1 cut(s) 529
BshNI GGYRCC 1 cut(s) 561
BshTI ACCGGT 1 cut(s) 1436
BshVI ATCGAT 1 cut(s) 1530
BsiEI CGRYCG 1 cut(s) 1057
BsiHKAI GWGCWC 3 cut(s) 1348, 1513, 1620
BsiHKCI CYCGRG 1 cut(s) 1244
BsiSI CCGG 2 cut(s) 1437, 1994
BslFI GGGAC 3 cut(s) 1175, 1227, 1393
BslI CCNNNNNNNGG 3 cut(s) 401, 557, 1386
BsmAI GTCTC 4 cut(s) 141, 786, 1636, 1828
BsmFI GGGAC 3 cut(s) 1175, 1227, 1393
BsmI GAATGC 1 cut(s) 1223
BsnI GGCC 1 cut(s) 529
Bso31I GGTCTC 2 cut(s) 141, 786
BsoBI CYCGRG 1 cut(s) 1244
Bsp119I TTCGAA 2 cut(s) 856, 1939
Bsp1286I GDGCHC 5 cut(s) 311, 564, 1348, 1513, 1620
Bsp1407I TGTACA 1 cut(s) 577
Bsp143I GATC 7 cut(s) 406, 466, 670, 751, 1048, 1054, 1527
Bsp1720I GCTNAGC 1 cut(s) 1655
Bsp19I CCATGG 2 cut(s) 158, 1958
BspACI CCGC 4 cut(s) 453, 768, 1029, 1863
BspANI GGCC 1 cut(s) 529
BspCNI CTCAG 3 cut(s) 169, 203, 1704
BspDI ATCGAT 1 cut(s) 1530
BspFNI CGCG 1 cut(s) 770
BspHI TCATGA 3 cut(s) 538, 595, 1135
BspLI GGNNCC 5 cut(s) 563, 753, 1116, 1191, 1409
BspMI ACCTGC 2 cut(s) 690, 1177
BspPI GGATC 5 cut(s) 461, 665, 746, 759, 1043
BspT104I TTCGAA 2 cut(s) 856, 1939
BspT107I GGYRCC 1 cut(s) 561
BspTI CTTAAG 1 cut(s) 1412
BspTNI GGTCTC 2 cut(s) 141, 786
BsrDI GCAATG 2 cut(s) 1024, 1750
BsrFI RCCGGY 1 cut(s) 1436
BsrGI TGTACA 1 cut(s) 577
BsrI ACTGG 2 cut(s) 1275, 1978
BssAI RCCGGY 1 cut(s) 1436
BssECI CCNNGG 5 cut(s) 158, 551, 746, 1321, 1958
BssMI GATC 7 cut(s) 406, 466, 670, 751, 1048, 1054, 1527
BssNAI GTATAC 1 cut(s) 644
BssT1I CCWWGG 3 cut(s) 158, 551, 1958
Bst1107I GTATAC 1 cut(s) 644
Bst2UI CCWGG 3 cut(s) 747, 778, 1322
Bst4CI ACNGT 7 cut(s) 199, 218, 1498, 1570, 1630, 1951, 2152
Bst6I CTCTTC 3 cut(s) 466, 1194, 1791
BstAFI CTTAAG 1 cut(s) 1412
BstAUI TGTACA 1 cut(s) 577
BstBAI YACGTR 1 cut(s) 616
BstBI TTCGAA 2 cut(s) 856, 1939
BstC8I GCNNGC 2 cut(s) 527, 1652
BstDEI CTNAG 5 cut(s) 177, 190, 1350, 1655, 1691
BstDSI CCRYGG 2 cut(s) 158, 1958
BstENI CCTNNNNNAGG 1 cut(s) 555
BstF5I GGATG 9 cut(s) 10, 280, 438, 449, 607, 1659, 1780, 1890, 2002
BstFNI CGCG 1 cut(s) 770
BstHHI GCGC 1 cut(s) 772
BstKTI GATC 7 cut(s) 409, 469, 673, 754, 1051, 1057, 1530
BstMAI GTCTC 4 cut(s) 141, 786, 1636, 1828
BstMBI GATC 7 cut(s) 406, 466, 670, 751, 1048, 1054, 1527
BstMCI CGRYCG 1 cut(s) 1057
BstMWI GCNNNNNNNGC 5 cut(s) 424, 1343, 1656, 1871, 1961
BstNI CCWGG 3 cut(s) 747, 778, 1322
BstNSI RCATGY 1 cut(s) 1917
BstSCI CCNGG 3 cut(s) 745, 776, 1320
BstSFI CTRYAG 3 cut(s) 214, 1701, 1950
BstSLI GKGCMC 1 cut(s) 564
BstSNI TACGTA 1 cut(s) 616
BstUI CGCG 1 cut(s) 770
BstV1I GCAGC 9 cut(s) 82, 405, 467, 1663, 1752, 1883, 1991, 1994, 2089
BstV2I GAAGAC 2 cut(s) 234, 936
BstX2I RGATCY 3 cut(s) 466, 670, 751
BstYI RGATCY 3 cut(s) 466, 670, 751
BstZ17I GTATAC 1 cut(s) 644
Bsu15I ATCGAT 1 cut(s) 1530
BsuI GTATCC 2 cut(s) 288, 1164
BsuRI GGCC 1 cut(s) 529
BsuTUI ATCGAT 1 cut(s) 1530
BtgI CCRYGG 2 cut(s) 158, 1958
BtsCI GGATG 9 cut(s) 10, 280, 438, 449, 607, 1659, 1780, 1890, 2002
BtsIMutI CAGTG 2 cut(s) 1503, 1971
BveI ACCTGC 2 cut(s) 690, 1177
Cac8I GCNNGC 2 cut(s) 527, 1652
CaiI CAGNNNCTG 2 cut(s) 197, 1321
CciI TCATGA 3 cut(s) 538, 595, 1135
CfoI GCGC 1 cut(s) 772
Cfr10I RCCGGY 1 cut(s) 1436
Cfr13I GGNCC 4 cut(s) 21, 798, 1189, 1407
ClaI ATCGAT 1 cut(s) 1530
CseI GACGC 1 cut(s) 53
Csp6I GTAC 5 cut(s) 578, 757, 1099, 1249, 1318
CspAI ACCGGT 1 cut(s) 1436
CviQI GTAC 5 cut(s) 578, 757, 1099, 1249, 1318
DdeI CTNAG 5 cut(s) 177, 190, 1350, 1655, 1691
DpnI GATC 7 cut(s) 408, 468, 672, 753, 1050, 1056, 1529
DpnII GATC 7 cut(s) 406, 466, 670, 751, 1048, 1054, 1527
DrdI GACNNNNNNGTC 1 cut(s) 938
DseDI GACNNNNNNGTC 1 cut(s) 938
Eam1104I CTCTTC 3 cut(s) 466, 1194, 1791
EarI CTCTTC 3 cut(s) 466, 1194, 1791
EciI GGCGGA 1 cut(s) 1852
Ecl136II GAGCTC 1 cut(s) 1618
Eco105I TACGTA 1 cut(s) 616
Eco130I CCWWGG 3 cut(s) 158, 551, 1958
Eco24I GRGCYC 2 cut(s) 311, 1620
Eco31I GGTCTC 2 cut(s) 141, 786
Eco47I GGWCC 4 cut(s) 21, 798, 1189, 1407
Eco53kI GAGCTC 1 cut(s) 1618
Eco57I CTGAAG 6 cut(s) 264, 447, 948, 1176, 1238, 1811
Eco88I CYCGRG 1 cut(s) 1244
EcoICRI GAGCTC 1 cut(s) 1618
EcoNI CCTNNNNNAGG 1 cut(s) 555
EcoO109I RGGNCCY 1 cut(s) 1189
EcoRI GAATTC 1 cut(s) 434
EcoRII CCWGG 3 cut(s) 745, 776, 1320
EcoT14I CCWWGG 3 cut(s) 158, 551, 1958
EcoT38I GRGCYC 2 cut(s) 311, 1620
ErhI CCWWGG 3 cut(s) 158, 551, 1958
FaqI GGGAC 3 cut(s) 1175, 1227, 1393
FblI GTMKAC 2 cut(s) 237, 643
Fnu4HI GCNGC 9 cut(s) 96, 419, 456, 1677, 1741, 1872, 1980, 1983, 2103
FokI GGATG 9 cut(s) 17, 287, 425, 436, 614, 1646, 1767, 1877, 2009
FriOI GRGCYC 2 cut(s) 311, 1620
Fsp4HI GCNGC 9 cut(s) 96, 419, 456, 1677, 1741, 1872, 1980, 1983, 2103
FspBI CTAG 7 cut(s) 74, 185, 1068, 1446, 1478, 1775, 2164
GlaI GCGC 1 cut(s) 771
GluI GCNGC 9 cut(s) 96, 419, 456, 1677, 1741, 1872, 1980, 1983, 2103
GsaI CCCAGC 2 cut(s) 427, 2102
HaeIII GGCC 1 cut(s) 529
HapII CCGG 2 cut(s) 1437, 1994
HgaI GACGC 1 cut(s) 53
HhaI GCGC 1 cut(s) 772
Hin6I GCGC 1 cut(s) 770
HinP1I GCGC 1 cut(s) 770
HincII GTYRAC 3 cut(s) 1065, 1772, 1969
HindII GTYRAC 3 cut(s) 1065, 1772, 1969
HindIII AAGCTT 1 cut(s) 1652
HinfI GANTC 8 cut(s) 80, 90, 170, 181, 188, 234, 1259, 1751
HpaII CCGG 2 cut(s) 1437, 1994
HphI GGTGA 7 cut(s) 416, 641, 821, 913, 1154, 1426, 2092
Hpy166II GTNNAC 5 cut(s) 238, 644, 1065, 1772, 1969
Hpy8I GTNNAC 5 cut(s) 238, 644, 1065, 1772, 1969
Hpy99I CGWCG 2 cut(s) 1049, 2049
HpyAV CCTTC 7 cut(s) 117, 497, 551, 1133, 1262, 1786, 2013
HpyCH4III ACNGT 7 cut(s) 199, 218, 1498, 1570, 1630, 1951, 2152
HpyCH4IV ACGT 3 cut(s) 298, 615, 635
HpyF10VI GCNNNNNNNGC 5 cut(s) 424, 1343, 1656, 1871, 1961
HpyF3I CTNAG 5 cut(s) 177, 190, 1350, 1655, 1691
HpySE526I ACGT 3 cut(s) 298, 615, 635
HspAI GCGC 1 cut(s) 770
Kzo9I GATC 7 cut(s) 406, 466, 670, 751, 1048, 1054, 1527
LmnI GCTCC 5 cut(s) 1312, 1351, 1615, 1697, 1883
Lsp1109I GCAGC 9 cut(s) 82, 405, 467, 1663, 1752, 1883, 1991, 1994, 2089
LweI GCATC 5 cut(s) 37, 265, 1668, 1805, 2011
MaeI CTAG 7 cut(s) 74, 185, 1068, 1446, 1478, 1775, 2164
MaeII ACGT 3 cut(s) 298, 615, 635
MaeIII GTNAC 5 cut(s) 145, 1432, 1514, 1684, 2080
MalI GATC 7 cut(s) 408, 468, 672, 753, 1050, 1056, 1529
MboI GATC 7 cut(s) 406, 466, 670, 751, 1048, 1054, 1527
MfeI CAATTG 1 cut(s) 495
MflI RGATCY 3 cut(s) 466, 670, 751
MhlI GDGCHC 5 cut(s) 311, 564, 1348, 1513, 1620
MlyI GAGTC 3 cut(s) 182, 243, 1253
MmeI TCCRAC 1 cut(s) 252
MseI TTAA 6 cut(s) 315, 414, 588, 677, 1413, 1557
MslI CAYNNNNRTG 7 cut(s) 130, 600, 968, 1140, 1506, 1918, 2007
MspA1I CMGCKG 1 cut(s) 2102
MspCI CTTAAG 1 cut(s) 1412
MspI CCGG 2 cut(s) 1437, 1994
MspR9I CCNGG 3 cut(s) 747, 778, 1322
MunI CAATTG 1 cut(s) 495
Mva1269I GAATGC 1 cut(s) 1223
MvaI CCWGG 3 cut(s) 747, 778, 1322
MvnI CGCG 1 cut(s) 770
MwoI GCNNNNNNNGC 5 cut(s) 424, 1343, 1656, 1871, 1961
NcoI CCATGG 2 cut(s) 158, 1958
NdeII GATC 7 cut(s) 406, 466, 670, 751, 1048, 1054, 1527
NlaIV GGNNCC 5 cut(s) 563, 753, 1116, 1191, 1409
NmuCI GTSAC 4 cut(s) 145, 1432, 1684, 2080
NspI RCATGY 1 cut(s) 1917
NspV TTCGAA 2 cut(s) 856, 1939
PagI TCATGA 3 cut(s) 538, 595, 1135
PaqCI CACCTGC 1 cut(s) 690
PcsI WCGNNNNNNNCGW 2 cut(s) 148, 1050
PctI GAATGC 1 cut(s) 1223
PfeI GAWTC 5 cut(s) 80, 90, 170, 181, 1751
PflFI GACNNNGTC 1 cut(s) 233
PinAI ACCGGT 1 cut(s) 1436
PkrI GCNGC 9 cut(s) 97, 420, 457, 1678, 1742, 1873, 1981, 1984, 2104
Ple19I CGATCG 1 cut(s) 1057
PleI GAGTC 3 cut(s) 182, 242, 1253
PpsI GAGTC 3 cut(s) 182, 242, 1253
Ppu21I YACGTR 1 cut(s) 616
PpuMI RGGWCCY 1 cut(s) 1189
PshBI ATTAAT 1 cut(s) 315
PsiI TTATAA 1 cut(s) 888
Psp124BI GAGCTC 1 cut(s) 1620
Psp5II RGGWCCY 1 cut(s) 1189
Psp6I CCWGG 3 cut(s) 745, 776, 1320
PspFI CCCAGC 2 cut(s) 423, 2098
PspGI CCWGG 3 cut(s) 745, 776, 1320
PspN4I GGNNCC 5 cut(s) 563, 753, 1116, 1191, 1409
PspPI GGNCC 4 cut(s) 21, 798, 1189, 1407
PspPPI RGGWCCY 1 cut(s) 1189
PstNI CAGNNNCTG 2 cut(s) 197, 1321
PsuI RGATCY 3 cut(s) 466, 670, 751
PsyI GACNNNGTC 1 cut(s) 233
PvuI CGATCG 1 cut(s) 1057
PvuII CAGCTG 1 cut(s) 2102
RsaI GTAC 5 cut(s) 579, 758, 1100, 1250, 1319
RsaNI GTAC 5 cut(s) 578, 757, 1099, 1249, 1318
RseI CAYNNNNRTG 7 cut(s) 130, 600, 968, 1140, 1506, 1918, 2007
SacI GAGCTC 1 cut(s) 1620
SaqAI TTAA 6 cut(s) 315, 414, 588, 677, 1413, 1557
SatI GCNGC 9 cut(s) 96, 419, 456, 1677, 1741, 1872, 1980, 1983, 2103
Sau3AI GATC 7 cut(s) 406, 466, 670, 751, 1048, 1054, 1527
Sau96I GGNCC 4 cut(s) 21, 798, 1189, 1407
SchI GAGTC 3 cut(s) 182, 243, 1253
ScrFI CCNGG 3 cut(s) 747, 778, 1322
SduI GDGCHC 5 cut(s) 311, 564, 1348, 1513, 1620
SfaNI GCATC 5 cut(s) 37, 265, 1668, 1805, 2011
SfcI CTRYAG 3 cut(s) 214, 1701, 1950
SfuI TTCGAA 2 cut(s) 856, 1939
SgrAI CRCCGGYG 1 cut(s) 1436
SinI GGWCC 4 cut(s) 21, 798, 1189, 1407
SmiMI CAYNNNNRTG 7 cut(s) 130, 600, 968, 1140, 1506, 1918, 2007
SmlI CTYRAG 3 cut(s) 245, 1412, 1726
SmoI CTYRAG 3 cut(s) 245, 1412, 1726
SnaBI TACGTA 1 cut(s) 616
SpeI ACTAGT 1 cut(s) 1067
SsiI CCGC 4 cut(s) 453, 768, 1029, 1863
SspI AATATT 2 cut(s) 355, 1081
SspMI CTAG 7 cut(s) 74, 185, 1068, 1446, 1478, 1775, 2164
SstI GAGCTC 1 cut(s) 1620
StyD4I CCNGG 3 cut(s) 745, 776, 1320
StyI CCWWGG 3 cut(s) 158, 551, 1958
TaaI ACNGT 7 cut(s) 199, 218, 1498, 1570, 1630, 1951, 2152
TaiI ACGT 3 cut(s) 301, 618, 638
TaqII GACCGA 1 cut(s) 446
TatI WGTACW 1 cut(s) 577
TfiI GAWTC 5 cut(s) 80, 90, 170, 181, 1751
Tru1I TTAA 6 cut(s) 315, 414, 588, 677, 1413, 1557
Tru9I TTAA 6 cut(s) 315, 414, 588, 677, 1413, 1557
TscAI CASTG 2 cut(s) 1503, 1978
TseFI GTSAC 4 cut(s) 145, 1432, 1684, 2080
TseI GCWGC 9 cut(s) 95, 418, 455, 1676, 1740, 1871, 1979, 1982, 2102
Tsp45I GTSAC 4 cut(s) 145, 1432, 1684, 2080
TspGWI ACGGA 1 cut(s) 744
TspRI CASTG 2 cut(s) 1503, 1978
Tth111I GACNNNGTC 1 cut(s) 233
Vha464I CTTAAG 1 cut(s) 1412
VpaK11BI GGWCC 4 cut(s) 21, 798, 1189, 1407
VspI ATTAAT 1 cut(s) 315
XagI CCTNNNNNAGG 1 cut(s) 555
XapI RAATTY 6 cut(s) 434, 511, 590, 1033, 1906, 2067
XbaI TCTAGA 1 cut(s) 184
XceI RCATGY 1 cut(s) 1917
XcmI CCANNNNNNNNNTGG 1 cut(s) 792
XmiI GTMKAC 2 cut(s) 237, 643
XspI CTAG 7 cut(s) 74, 185, 1068, 1446, 1478, 1775, 2164
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.