Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb6

Orthogroup ID

OG0000142

Physical Location & Seq

Genomic Coordinates Reverse (-)

36960158 .. 36961764

Gene Structure 1607 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_6g49280.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1137 bp

ATGTTGAGCAGATGGAGCCTTTCAAGAAAGTTGATATGGTGTCGTGATTTGATACGCCGCAAGTCTGATACATTCTTCACTAGAAAACGATACAAGTCGGAAGAAGAACTCAATGAGGGCAGGTGCTTCATGCAGGAACTCCCAGATGAGGTCATGCAACTGATTCTGCAGAGACTGCCCCTATTAGATTATCTGAATTGTCGTGACGTAAGTCGTTGTTGGCGAGCCGCAATTGACAGCAAGCGTTGGCTTCCTGCCGCTCATCATCGACTCCCAAGGCTTGTGACCAGCTCTACTTTAGGAAGATGCATGCAGGCATCCTACATCAAAACAGATGATGACGACGACGACTTGCGTATTGTATGCAATCCGAATATTTGGAATCCTGACGCTCCATACTGTTATTGTGCTGGATCAATCGAGGGATGGCTCATCATAACACTTGAGAGAACATTTGTCAAATTCTTCTTCTGGAATCCAGTATCAGGTGCTCGAGTTAAGCTCCCATCCCGCCGCATGTCGATCTTGCCGCCAGGAAAAGTGGTAGCCTCTTCGGTACCAACGTCGACAACAACAAAGTGTTATGTGAGTTGCCTTCACTTTAAACAAGGATGTTGTTTGGCCTTCTGCACCCCCTCTGATAAAACATGGACCGTGATTGAGAAAGGTCAAAAATTTTGGGATATAGAAATACTTGATGGGAAACTATACGCGGTAGGAAGCCATGCATTAGAGTTTGTGATGGTGTTTGACATCCATTTAGACACCCATACCTACACATCTCAAAGGTTGGTTACTCTTCACCCCAACATTAAAAACATATATGTTGATGACGAAACCCTCTACCAGACATTTTACTTGGCAAAATGTTGTGAATCAAAGGACCTGTTTATGGTTTTGTATCAAAATCAAAGCAACGGATTCGAAGGATTGTTTCAAGTGTTGAAGTTAGAGTATAATAATGTTACCACCCGTCATCAGTGGGGAAATAGATACACCTCCGGTGTGTTTTCCTTGACAGATAGGAGCTTTAAGCCTATGTCTCTACCCGAGGAGACTAAAGTTAATAAGAAATTACCACCCATTACAGAATGGGGGAAGGGTGATGAACTGCTATTTACTAAGCCTGAGCAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000463 GO:0000470 GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0006139 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0010467 GO:0010468 GO:0016070 GO:0016072 GO:0019219 GO:0019222 GO:0022613 GO:0030684 GO:0030686 GO:0031323 GO:0031974 GO:0031981 GO:0032991 GO:0034470 GO:0034641 GO:0034660 GO:0042254 GO:0042273 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044087 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044452 GO:0044464 GO:0046483 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0070013 GO:0070545 GO:0071704 GO:0071840 GO:0080090 GO:0090069 GO:0090304 GO:0097159 GO:1901360 GO:1901363 GO:1990904 GO:2000232

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

BRCT_2 Pescadillo_N

Protein Analysis

379

Amino Acids

44.06

Weight (kDa)

8.63

Isoelectric Point (pI)

48.06

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
F-box	PF00646	45 - 82	7.7e-10	F-box domain
F-box-like	PF12937	45 - 80	8.1e-08	F-box-like
Beta-prop_KIB1-4	PF03478	129 - 329	2.9e-23	KIB1-4 beta-propeller

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000142)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g13532 FvH4_2g28870 FvH4_3g30930 FvH4_3g37281 FvH4_3g37300 FvH4_3g37301 FvH4_3g37301 FvH4_4g23711 FvH4_5g02330 FvH4_6g16960 FvH4_6g16971 FvH4_6g21291 FvH4_6g21331 FvH4_6g22212 FvH4_6g22332 FvH4_6g36920 FvH4_6g37552 FvH4_6g37591 FvH4_6g39721 FvH4_6g46020 FvH4_6g47001 FvH4_6g47002 FvH4_6g47130 FvH4_6g47140 FvH4_6g47161 FvH4_6g47170 FvH4_6g47190 FvH4_6g49120 FvH4_6g49280 FvH4_6g49281 FvH4_6g49640 FvH4_6g49640
malus_domestica	MD03G1085600.v1.1 MD09G1027500.v1.1 MD09G1050100.v1.1
prunus_persica	Prupe.1G004300_v2.0.a1 Prupe.1G016300_v2.0.a1 Prupe.1G019300_v2.0.a1 Prupe.3G239100_v2.0.a1 Prupe.3G291200_v2.0.a1
pyrus_communis	pycom08g02840 pycom08g02860 pycom08g02870
rosa_chinensis	RchiOBHm_Chr2g0153831 RchiOBHm_Chr2g0166171 RchiOBHm_Chr2g0166201 RchiOBHm_Chr3g0476221 RchiOBHm_Chr4g0430971 RchiOBHm_Chr5g0067081 RchiOBHm_Chr7g0197821
rosa_laevigata	RLG00000001284 RLG00000006996 RLG00000012710 RLG00000019299 RLG00000020285 RLG00000020444 RLG00000020466 RLG00000020591 RLG00000020665 RLG00000020730 RLG00000020732 RLG00000021449 RLG00000021592 RLG00000021593 RLG00000021594 RLG00000021596 RLG00000021597 RLG00000021801 RLG00000023534 RLG00000023538 RLG00000023693 RLG00000023694 RLG00000023699 RLG00000024196 RLG00000024214 RLG00000024220 RLG00000024244 RLG00000024608 RLG00000024612 RLG00000035157 RLG00000035906
rosa_multiflora	Rmu_co8282815.1_g000001 Rmu_co8380495.1_g000001 Rmu_co8381111.1_g000001 Rmu_co8460513.1_g000001 Rmu_co8481947.1_g000001 Rmu_co8487937.1_g000001 Rmu_sc0000151.1_g000017 Rmu_sc0001427.1_g000003 Rmu_sc0001427.1_g000006 Rmu_sc0002611.1_g000008 Rmu_sc0003887.1_g000018 Rmu_sc0003887.1_g000019 Rmu_sc0003887.1_g000021 Rmu_sc0004119.1_g000004 Rmu_sc0004329.1_g000003 Rmu_sc0004485.1_g000024 Rmu_sc0005290.1_g000001 Rmu_sc0005609.1_g000006 Rmu_sc0006187.1_g000018 Rmu_sc0006218.1_g000032 Rmu_sc0006218.1_g000033 Rmu_sc0007150.1_g000003 Rmu_sc0007154.1_g000004 Rmu_sc0007154.1_g000005 Rmu_sc0007154.1_g000007 Rmu_sc0008314.1_g000012 Rmu_sc0010073.1_g000023 Rmu_sc0010073.1_g000025 Rmu_sc0011216.1_g000008 Rmu_sc0012445.1_g000005 Rmu_sc0013571.1_g000002 Rmu_sc0018648.1_g000002 Rmu_sc0020961.1_g000002 Rmu_sc0020961.1_g000003 Rmu_sc0023710.1_g000001 Rmu_sc0031310.1_g000008 Rmu_sc0033940.1_g000001 Rmu_sc0035493.1_g000001 Rmu_sc0039285.1_g000001
rosa_roxburghii	Rroxscaffold_1G00014000 Rroxscaffold_1G00014020 Rroxscaffold_2G00082760 Rroxscaffold_2G00085090 Rroxscaffold_2G00085100 Rroxscaffold_2G00085140 Rroxscaffold_2G00086670 Rroxscaffold_2G00086680 Rroxscaffold_2G00098190 Rroxscaffold_2G00099270 Rroxscaffold_3G00227460 Rroxscaffold_3G00258190 Rroxscaffold_3G00259780 Rroxscaffold_6G00402940 Rroxscaffold_6G00410200 Rroxscaffold_6G00410210 Rroxscaffold_7G00184320
rosa_rugosa	Rorug02G0416100 Rorug02G0416200 Rorug02G0520900 Rorug02G0521200 Rorug02G0521300 Rorug02G0534600 Rorug03G0116300 Rorug03G0162900 Rorug03G0162900 Rorug04G0241400 Rorug05G0380900 Rorug07G0043800
rosa_samantha	Rh1AG115300 Rh2BG483700 Rh2BG509900 Rh2BG515700 Rh2BG515800 Rh2BG586500 Rh2BG586600 Rh2BG586700 Rh2BG598800 Rh2BG598900 Rh2BG599100 Rh2BG618400 Rh2BG630500 Rh2CG457400 Rh2CG484900 Rh3AG165300 Rh3AG212900 Rh3AG213300 Rh3BG145800 Rh3BG146100 Rh3BG235300 Rh3BG246500 Rh3BG247000 Rh3BG247100 Rh3CG240300 Rh3CG240800 Rh3CG240900 Rh3CG259600 Rh3DG147000 Rh3DG184800 Rh3DG184900 Rh3DG185000 Rh3DG239500 Rh3DG240100 Rh3DG240200 Rh3DG255800 Rh5BG458500 Rh5CG480400 Rh7BG170400 Rh7BG407900 Rh7CG176900
rosa_wichuraiana	Rw2G038990 Rw2G041550 Rw2G047660 Rw2G048940 Rw4G026370 Rw6G008730 Rw7G014600

Restriction Enzyme Sites

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Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	111
Acc36I	ACCTGC	1 cut(s)	111
Acc65I	GGTACC	1 cut(s)	556
AccB1I	GGYRCC	1 cut(s)	556
AccBSI	CCGCTC	1 cut(s)	260
AccI	GTMKAC	1 cut(s)	566
AccII	CGCG	1 cut(s)	713
AciI	CCGC	7 cut(s)	58, 228, 258, 511, 514, 530, 713
AclWI	GGATC	1 cut(s)	421
AcsI	RAATTY	2 cut(s)	461, 674
AfaI	GTAC	1 cut(s)	558
AfiI	CCNNNNNNNGG	3 cut(s)	148, 485, 892
AgsI	TTSAA	3 cut(s)	24, 938, 946
AjnI	CCWGG	1 cut(s)	532
AluBI	AGCT	3 cut(s)	291, 502, 1029
AluI	AGCT	3 cut(s)	291, 502, 1029
Alw21I	GWGCWC	1 cut(s)	493
Alw26I	GTCTC	3 cut(s)	166, 1047, 1049
AlwI	GGATC	1 cut(s)	421
AlwNI	CAGNNNCTG	1 cut(s)	175
Ama87I	CYCGRG	2 cut(s)	492, 1049
AoxI	GGCC	1 cut(s)	621
ApoI	RAATTY	2 cut(s)	461, 674
Asp718I	GGTACC	1 cut(s)	556
AspS9I	GGNCC	2 cut(s)	651, 883
AsuHPI	GGTGA	2 cut(s)	794, 1115
AsuII	TTCGAA	1 cut(s)	924
AvaI	CYCGRG	2 cut(s)	492, 1049
AvaII	GGWCC	2 cut(s)	651, 883
BaeI	ACNNNNGTAYC	2 cut(s)	548, 581
BanI	GGYRCC	1 cut(s)	556
Bbv12I	GWGCWC	1 cut(s)	493
BccI	CCATC	5 cut(s)	6, 420, 514, 692, 736
BcgI	CGANNNNNNTGC	2 cut(s)	904, 938
BciT130I	CCWGG	1 cut(s)	534
BcoDI	GTCTC	3 cut(s)	166, 1047, 1049
BfaI	CTAG	1 cut(s)	81
BfmI	CTRYAG	1 cut(s)	167
BfuAI	ACCTGC	1 cut(s)	111
BisI	GCNGC	5 cut(s)	58, 228, 258, 514, 530
BlsI	GCNGC	5 cut(s)	59, 229, 259, 515, 531
Bme1390I	CCNGG	1 cut(s)	534
Bme18I	GGWCC	2 cut(s)	651, 883
BmeT110I	CYCGRG	2 cut(s)	492, 1049
BmgT120I	GGNCC	2 cut(s)	651, 883
BmiI	GGNNCC	2 cut(s)	17, 558
BmrFI	CCNGG	1 cut(s)	534
BmsI	GCATC	2 cut(s)	296, 326
BoxI	GACNNNNGTC	1 cut(s)	210
BplI	GAGNNNNNCTC	2 cut(s)	486, 518
Bpu10I	CCTNAGC	1 cut(s)	1128
Bpu14I	TTCGAA	1 cut(s)	924
BpuEI	CTTGAG	1 cut(s)	464
BsaJI	CCNNGG	2 cut(s)	275, 1050
BsaWI	WCCGGW	1 cut(s)	1001
Bsc4I	CCNNNNNNNGG	3 cut(s)	148, 485, 892
Bse1I	ACTGG	1 cut(s)	479
BseBI	CCWGG	1 cut(s)	534
BseDI	CCNNGG	2 cut(s)	275, 1050
BseGI	GGATG	5 cut(s)	317, 431, 506, 617, 753
BseLI	CCNNNNNNNGG	3 cut(s)	148, 485, 892
BseMII	CTCAG	1 cut(s)	1119
BseNI	ACTGG	1 cut(s)	479
BseRI	GAGGAG	1 cut(s)	1067
BsgI	GTGCAG	1 cut(s)	613
Bsh1236I	CGCG	1 cut(s)	713
BshFI	GGCC	1 cut(s)	623
BshNI	GGYRCC	1 cut(s)	556
BsiHKAI	GWGCWC	1 cut(s)	493
BsiHKCI	CYCGRG	2 cut(s)	492, 1049
BsiSI	CCGG	1 cut(s)	1002
BslI	CCNNNNNNNGG	3 cut(s)	148, 485, 892
BsmAI	GTCTC	3 cut(s)	166, 1047, 1049
BsnI	GGCC	1 cut(s)	623
BsoBI	CYCGRG	2 cut(s)	492, 1049
Bsp119I	TTCGAA	1 cut(s)	924
Bsp1286I	GDGCHC	1 cut(s)	493
Bsp143I	GATC	2 cut(s)	413, 522
BspACI	CCGC	7 cut(s)	58, 228, 258, 511, 514, 530, 713
BspANI	GGCC	1 cut(s)	623
BspCNI	CTCAG	1 cut(s)	1120
BspFNI	CGCG	1 cut(s)	713
BspLI	GGNNCC	2 cut(s)	17, 558
BspMAI	CTGCAG	1 cut(s)	171
BspMI	ACCTGC	1 cut(s)	111
BspPI	GGATC	1 cut(s)	421
BspT104I	TTCGAA	1 cut(s)	924
BspT107I	GGYRCC	1 cut(s)	556
BsrBI	CCGCTC	1 cut(s)	260
BsrI	ACTGG	1 cut(s)	479
BssECI	CCNNGG	2 cut(s)	275, 1050
BssMI	GATC	2 cut(s)	413, 522
BssT1I	CCWWGG	1 cut(s)	275
Bst2UI	CCWGG	1 cut(s)	534
Bst4CI	ACNGT	2 cut(s)	401, 655
Bst6I	CTCTTC	2 cut(s)	556, 804
BstAPI	GCANNNNNTGC	1 cut(s)	175
BstBI	TTCGAA	1 cut(s)	924
BstC8I	GCNNGC	4 cut(s)	225, 242, 311, 315
BstDEI	CTNAG	2 cut(s)	1122, 1128
BstF5I	GGATG	5 cut(s)	317, 431, 506, 617, 753
BstFNI	CGCG	1 cut(s)	713
BstKTI	GATC	2 cut(s)	416, 525
BstMAI	GTCTC	3 cut(s)	166, 1047, 1049
BstMBI	GATC	2 cut(s)	413, 522
BstMWI	GCNNNNNNNGC	2 cut(s)	15, 175
BstNI	CCWGG	1 cut(s)	534
BstNSI	RCATGY	2 cut(s)	313, 520
BstPAI	GACNNNNGTC	1 cut(s)	210
BstSCI	CCNGG	1 cut(s)	532
BstSFI	CTRYAG	1 cut(s)	167
BstUI	CGCG	1 cut(s)	713
BsuRI	GGCC	1 cut(s)	623
BtsCI	GGATG	5 cut(s)	317, 431, 506, 617, 753
BtsIMutI	CAGTG	1 cut(s)	986
BveI	ACCTGC	1 cut(s)	111
Cac8I	GCNNGC	4 cut(s)	225, 242, 311, 315
CaiI	CAGNNNCTG	1 cut(s)	175
Cfr13I	GGNCC	2 cut(s)	651, 883
CseI	GACGC	1 cut(s)	398
Csp6I	GTAC	1 cut(s)	557
CviAII	CATG	6 cut(s)	130, 154, 310, 517, 648, 725
CviQI	GTAC	1 cut(s)	557
DdeI	CTNAG	2 cut(s)	1122, 1128
DpnI	GATC	2 cut(s)	415, 524
DpnII	GATC	2 cut(s)	413, 522
DraI	TTTAAA	1 cut(s)	604
Eam1104I	CTCTTC	2 cut(s)	556, 804
EarI	CTCTTC	2 cut(s)	556, 804
Eco130I	CCWWGG	1 cut(s)	275
Eco47I	GGWCC	2 cut(s)	651, 883
Eco88I	CYCGRG	2 cut(s)	492, 1049
EcoO109I	RGGNCCY	1 cut(s)	883
EcoRII	CCWGG	1 cut(s)	532
EcoT14I	CCWWGG	1 cut(s)	275
EcoT22I	ATGCAT	2 cut(s)	311, 730
ErhI	CCWWGG	1 cut(s)	275
FaeI	CATG	6 cut(s)	133, 157, 313, 520, 651, 728
FatI	CATG	6 cut(s)	129, 153, 309, 516, 647, 724
FauI	CCCGC	1 cut(s)	518
FblI	GTMKAC	1 cut(s)	566
Fnu4HI	GCNGC	5 cut(s)	58, 228, 258, 514, 530
FokI	GGATG	5 cut(s)	304, 438, 493, 624, 740
Fsp4HI	GCNGC	5 cut(s)	58, 228, 258, 514, 530
FspBI	CTAG	1 cut(s)	81
GluI	GCNGC	5 cut(s)	58, 228, 258, 514, 530
HaeIII	GGCC	1 cut(s)	623
HapII	CCGG	1 cut(s)	1002
HgaI	GACGC	1 cut(s)	398
Hin1II	CATG	6 cut(s)	133, 157, 313, 520, 651, 728
HincII	GTYRAC	1 cut(s)	567
HindII	GTYRAC	1 cut(s)	567
HinfI	GANTC	6 cut(s)	163, 270, 382, 475, 875, 921
HpaII	CCGG	1 cut(s)	1002
HphI	GGTGA	2 cut(s)	794, 1115
Hpy166II	GTNNAC	1 cut(s)	567
Hpy188I	TCNGA	5 cut(s)	67, 100, 195, 372, 640
Hpy188III	TCNNGA	5 cut(s)	24, 44, 203, 386, 472
Hpy8I	GTNNAC	1 cut(s)	567
Hpy99I	CGWCG	3 cut(s)	347, 350, 568
HpyAV	CCTTC	4 cut(s)	605, 634, 920, 1093
HpyCH4III	ACNGT	2 cut(s)	401, 655
HpyCH4IV	ACGT	2 cut(s)	207, 563
HpyCH4V	TGCA	8 cut(s)	133, 157, 169, 309, 313, 366, 630, 728
HpyF10VI	GCNNNNNNNGC	2 cut(s)	15, 175
HpyF3I	CTNAG	2 cut(s)	1122, 1128
HpySE526I	ACGT	2 cut(s)	207, 563
Hsp92II	CATG	6 cut(s)	133, 157, 313, 520, 651, 728
KpnI	GGTACC	1 cut(s)	560
Kzo9I	GATC	2 cut(s)	413, 522
LmnI	GCTCC	4 cut(s)	15, 397, 507, 1026
LweI	GCATC	2 cut(s)	296, 326
MaeI	CTAG	1 cut(s)	81
MaeII	ACGT	2 cut(s)	207, 563
MaeIII	GTNAC	4 cut(s)	203, 283, 793, 964
MalI	GATC	2 cut(s)	415, 524
MbiI	CCGCTC	1 cut(s)	260
MboI	GATC	2 cut(s)	413, 522
MboII	GAAGA	8 cut(s)	67, 113, 116, 315, 457, 460, 543, 791
MfeI	CAATTG	1 cut(s)	231
MhlI	GDGCHC	1 cut(s)	493
MluCI	AATT	5 cut(s)	196, 231, 461, 674, 1073
MlyI	GAGTC	1 cut(s)	264
MmeI	TCCRAC	1 cut(s)	78
MnlI	CCTC	8 cut(s)	109, 142, 415, 559, 646, 851, 1009, 1045
Mph1103I	ATGCAT	2 cut(s)	311, 730
MseI	TTAA	5 cut(s)	498, 603, 813, 1032, 1065
MspI	CCGG	1 cut(s)	1002
MspR9I	CCNGG	1 cut(s)	534
MunI	CAATTG	1 cut(s)	231
MvaI	CCWGG	1 cut(s)	534
MvnI	CGCG	1 cut(s)	713
MwoI	GCNNNNNNNGC	2 cut(s)	15, 175
NdeII	GATC	2 cut(s)	413, 522
NlaIII	CATG	6 cut(s)	133, 157, 313, 520, 651, 728
NlaIV	GGNNCC	2 cut(s)	17, 558
NmuCI	GTSAC	2 cut(s)	203, 283
NsiI	ATGCAT	2 cut(s)	311, 730
NspI	RCATGY	2 cut(s)	313, 520
NspV	TTCGAA	1 cut(s)	924
PaeI	GCATGC	1 cut(s)	313
PaeR7I	CTCGAG	1 cut(s)	492
PaqCI	CACCTGC	1 cut(s)	111
PcsI	WCGNNNNNNNCGW	2 cut(s)	220, 560
PfeI	GAWTC	5 cut(s)	163, 382, 475, 875, 921
PkrI	GCNGC	5 cut(s)	59, 229, 259, 515, 531
PleI	GAGTC	1 cut(s)	264
PpsI	GAGTC	1 cut(s)	264
PpuMI	RGGWCCY	1 cut(s)	883
PshAI	GACNNNNGTC	1 cut(s)	210
Psp5II	RGGWCCY	1 cut(s)	883
Psp6I	CCWGG	1 cut(s)	532
PspGI	CCWGG	1 cut(s)	532
PspN4I	GGNNCC	2 cut(s)	17, 558
PspPI	GGNCC	2 cut(s)	651, 883
PspPPI	RGGWCCY	1 cut(s)	883
PspXI	VCTCGAGB	1 cut(s)	492
PstI	CTGCAG	1 cut(s)	171
PstNI	CAGNNNCTG	1 cut(s)	175
RsaI	GTAC	1 cut(s)	558
RsaNI	GTAC	1 cut(s)	557
SalI	GTCGAC	1 cut(s)	565
SaqAI	TTAA	5 cut(s)	498, 603, 813, 1032, 1065
SatI	GCNGC	5 cut(s)	58, 228, 258, 514, 530
Sau3AI	GATC	2 cut(s)	413, 522
Sau96I	GGNCC	2 cut(s)	651, 883
SchI	GAGTC	1 cut(s)	264
ScrFI	CCNGG	1 cut(s)	534
SduI	GDGCHC	1 cut(s)	493
SfaNI	GCATC	2 cut(s)	296, 326
SfcI	CTRYAG	1 cut(s)	167
Sfr274I	CTCGAG	1 cut(s)	492
SfuI	TTCGAA	1 cut(s)	924
SinI	GGWCC	2 cut(s)	651, 883
SlaI	CTCGAG	1 cut(s)	492
SmlI	CTYRAG	2 cut(s)	443, 492
SmoI	CTYRAG	2 cut(s)	443, 492
SphI	GCATGC	1 cut(s)	313
Sse9I	AATT	5 cut(s)	196, 231, 461, 674, 1073
SsiI	CCGC	7 cut(s)	58, 228, 258, 511, 514, 530, 713
SspI	AATATT	1 cut(s)	376
SspMI	CTAG	1 cut(s)	81
StyD4I	CCNGG	1 cut(s)	532
StyI	CCWWGG	1 cut(s)	275
TaaI	ACNGT	2 cut(s)	401, 655
TaiI	ACGT	2 cut(s)	210, 566
TaqI	TCGA	6 cut(s)	268, 420, 493, 521, 566, 924
TasI	AATT	5 cut(s)	196, 231, 461, 674, 1073
TauI	GCSGC	5 cut(s)	60, 230, 260, 516, 532
TfiI	GAWTC	5 cut(s)	163, 382, 475, 875, 921
Tru1I	TTAA	5 cut(s)	498, 603, 813, 1032, 1065
Tru9I	TTAA	5 cut(s)	498, 603, 813, 1032, 1065
TscAI	CASTG	1 cut(s)	986
TseFI	GTSAC	2 cut(s)	203, 283
Tsp45I	GTSAC	2 cut(s)	203, 283
TspDTI	ATGAA	2 cut(s)	118, 1122
TspGWI	ACGGA	1 cut(s)	933
TspRI	CASTG	1 cut(s)	986
VpaK11BI	GGWCC	2 cut(s)	651, 883
XapI	RAATTY	2 cut(s)	461, 674
XceI	RCATGY	2 cut(s)	313, 520
XhoI	CTCGAG	1 cut(s)	492
XmiI	GTMKAC	1 cut(s)	566
XspI	CTAG	1 cut(s)	81
Zsp2I	ATGCAT	2 cut(s)	311, 730

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.