Required for maturation of ribosomal RNAs and formation of the large ribosomal subunit
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 295 |
| AciI | CCGC | 2 cut(s) | 141, 406 |
| AcsI | RAATTY | 3 cut(s) | 737, 811, 826 |
| AcuI | CTGAAG | 1 cut(s) | 774 |
| AcvI | CACGTG | 1 cut(s) | 458 |
| AfaI | GTAC | 5 cut(s) | 92, 263, 725, 732, 839 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 291, 645 |
| AgsI | TTSAA | 3 cut(s) | 366, 668, 826 |
| AjnI | CCWGG | 3 cut(s) | 164, 232, 770 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 270, 302, 860, 892 |
| AluBI | AGCT | 6 cut(s) | 389, 420, 644, 709, 1018, 1061 |
| AluI | AGCT | 6 cut(s) | 389, 420, 644, 709, 1018, 1061 |
| Alw26I | GTCTC | 1 cut(s) | 629 |
| Ama87I | CYCGRG | 1 cut(s) | 462 |
| AoxI | GGCC | 3 cut(s) | 605, 774, 1041 |
| ApeKI | GCWGC | 6 cut(s) | 472, 538, 541, 550, 569, 1025 |
| ApoI | RAATTY | 3 cut(s) | 737, 811, 826 |
| AspS9I | GGNCC | 3 cut(s) | 624, 774, 1041 |
| AsuHPI | GGTGA | 5 cut(s) | 222, 507, 760, 959, 992 |
| AsuII | TTCGAA | 2 cut(s) | 289, 886 |
| AvaI | CYCGRG | 1 cut(s) | 462 |
| AvaII | GGWCC | 1 cut(s) | 624 |
| BaeGI | GKGCMC | 2 cut(s) | 298, 463 |
| BanI | GGYRCC | 1 cut(s) | 295 |
| BanII | GRGCYC | 1 cut(s) | 651 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 698, 730 |
| BbrPI | CACGTG | 1 cut(s) | 458 |
| BbvI | GCAGC | 6 cut(s) | 459, 525, 553, 556, 562, 1012 |
| BccI | CCATC | 4 cut(s) | 16, 484, 922, 961 |
| BciT130I | CCWGG | 3 cut(s) | 166, 234, 772 |
| BciVI | GTATCC | 1 cut(s) | 707 |
| BclI | TGATCA | 1 cut(s) | 676 |
| BcoDI | GTCTC | 1 cut(s) | 629 |
| BfaI | CTAG | 3 cut(s) | 107, 848, 1058 |
| BfmI | CTRYAG | 1 cut(s) | 539 |
| BfuI | GTATCC | 1 cut(s) | 707 |
| BisI | GCNGC | 8 cut(s) | 141, 406, 473, 539, 542, 551, 570, 1026 |
| BlsI | GCNGC | 8 cut(s) | 142, 407, 474, 540, 543, 552, 571, 1027 |
| BmcAI | AGTACT | 1 cut(s) | 725 |
| Bme1390I | CCNGG | 3 cut(s) | 166, 234, 772 |
| Bme18I | GGWCC | 1 cut(s) | 624 |
| BmeT110I | CYCGRG | 1 cut(s) | 462 |
| BmgT120I | GGNCC | 3 cut(s) | 624, 774, 1041 |
| BmiI | GGNNCC | 2 cut(s) | 297, 626 |
| BmrFI | CCNGG | 3 cut(s) | 166, 234, 772 |
| BmsI | GCATC | 3 cut(s) | 244, 392, 872 |
| Bpu14I | TTCGAA | 2 cut(s) | 289, 886 |
| BsaAI | YACGTR | 1 cut(s) | 458 |
| BsaI | GGTCTC | 1 cut(s) | 629 |
| BsaJI | CCNNGG | 5 cut(s) | 194, 233, 771, 867, 976 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 26, 56, 266, 296 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 291, 645 |
| Bse1I | ACTGG | 2 cut(s) | 449, 738 |
| BseBI | CCWGG | 3 cut(s) | 166, 234, 772 |
| BseDI | CCNNGG | 5 cut(s) | 194, 233, 771, 867, 976 |
| BseGI | GGATG | 2 cut(s) | 804, 863 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 291, 645 |
| BseMII | CTCAG | 1 cut(s) | 1046 |
| BseNI | ACTGG | 2 cut(s) | 449, 738 |
| BseRI | GAGGAG | 2 cut(s) | 174, 1053 |
| BseSI | GKGCMC | 2 cut(s) | 298, 463 |
| BseXI | GCAGC | 6 cut(s) | 459, 525, 553, 556, 562, 1012 |
| BseYI | CCCAGC | 1 cut(s) | 644 |
| BshFI | GGCC | 3 cut(s) | 607, 776, 1043 |
| BshNI | GGYRCC | 1 cut(s) | 295 |
| BsiHKCI | CYCGRG | 1 cut(s) | 462 |
| BslFI | GGGAC | 1 cut(s) | 313 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 291, 645 |
| BsmAI | GTCTC | 1 cut(s) | 629 |
| BsmFI | GGGAC | 1 cut(s) | 313 |
| BsnI | GGCC | 3 cut(s) | 607, 776, 1043 |
| Bso31I | GGTCTC | 1 cut(s) | 629 |
| BsoBI | CYCGRG | 1 cut(s) | 462 |
| Bsp119I | TTCGAA | 2 cut(s) | 289, 886 |
| Bsp1286I | GDGCHC | 3 cut(s) | 298, 463, 651 |
| Bsp1407I | TGTACA | 2 cut(s) | 90, 261 |
| Bsp143I | GATC | 4 cut(s) | 311, 381, 676, 982 |
| Bsp19I | CCATGG | 1 cut(s) | 194 |
| BspACI | CCGC | 2 cut(s) | 141, 406 |
| BspANI | GGCC | 3 cut(s) | 607, 776, 1043 |
| BspCNI | CTCAG | 1 cut(s) | 1045 |
| BspLI | GGNNCC | 2 cut(s) | 297, 626 |
| BspMAI | CTGCAG | 1 cut(s) | 543 |
| BspT104I | TTCGAA | 2 cut(s) | 289, 886 |
| BspT107I | GGYRCC | 1 cut(s) | 295 |
| BspTNI | GGTCTC | 1 cut(s) | 629 |
| BsrGI | TGTACA | 2 cut(s) | 90, 261 |
| BsrI | ACTGG | 2 cut(s) | 449, 738 |
| BssECI | CCNNGG | 5 cut(s) | 194, 233, 771, 867, 976 |
| BssMI | GATC | 4 cut(s) | 311, 381, 676, 982 |
| BssT1I | CCWWGG | 3 cut(s) | 194, 867, 976 |
| Bst2UI | CCWGG | 3 cut(s) | 166, 234, 772 |
| Bst4CI | ACNGT | 4 cut(s) | 90, 427, 837, 947 |
| Bst6I | CTCTTC | 1 cut(s) | 517 |
| BstAUI | TGTACA | 2 cut(s) | 90, 261 |
| BstBAI | YACGTR | 1 cut(s) | 458 |
| BstBI | TTCGAA | 2 cut(s) | 289, 886 |
| BstC8I | GCNNGC | 1 cut(s) | 138 |
| BstDEI | CTNAG | 4 cut(s) | 15, 575, 998, 1032 |
| BstDSI | CCRYGG | 1 cut(s) | 194 |
| BstEII | GGTNACC | 1 cut(s) | 228 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 289 |
| BstF5I | GGATG | 2 cut(s) | 804, 863 |
| BstKTI | GATC | 4 cut(s) | 314, 384, 679, 985 |
| BstMAI | GTCTC | 1 cut(s) | 629 |
| BstMBI | GATC | 4 cut(s) | 311, 381, 676, 982 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 123, 547, 550, 1008 |
| BstNI | CCWGG | 3 cut(s) | 166, 234, 772 |
| BstPI | GGTNACC | 1 cut(s) | 228 |
| BstSCI | CCNGG | 3 cut(s) | 164, 232, 770 |
| BstSFI | CTRYAG | 1 cut(s) | 539 |
| BstSLI | GKGCMC | 2 cut(s) | 298, 463 |
| BstV1I | GCAGC | 6 cut(s) | 459, 525, 553, 556, 562, 1012 |
| BsuI | GTATCC | 1 cut(s) | 707 |
| BsuRI | GGCC | 3 cut(s) | 607, 776, 1043 |
| BtgI | CCRYGG | 1 cut(s) | 194 |
| BtsCI | GGATG | 2 cut(s) | 804, 863 |
| Cac8I | GCNNGC | 1 cut(s) | 138 |
| Cfr13I | GGNCC | 3 cut(s) | 624, 774, 1041 |
| CsiI | ACCWGGT | 1 cut(s) | 164 |
| Csp6I | GTAC | 5 cut(s) | 91, 262, 724, 731, 838 |
| CviAII | CATG | 4 cut(s) | 195, 469, 621, 1070 |
| CviQI | GTAC | 5 cut(s) | 91, 262, 724, 731, 838 |
| DdeI | CTNAG | 4 cut(s) | 15, 575, 998, 1032 |
| DpnI | GATC | 4 cut(s) | 313, 383, 678, 984 |
| DpnII | GATC | 4 cut(s) | 311, 381, 676, 982 |
| Eam1104I | CTCTTC | 1 cut(s) | 517 |
| EarI | CTCTTC | 1 cut(s) | 517 |
| Eco130I | CCWWGG | 3 cut(s) | 194, 867, 976 |
| Eco24I | GRGCYC | 1 cut(s) | 651 |
| Eco31I | GGTCTC | 1 cut(s) | 629 |
| Eco47I | GGWCC | 1 cut(s) | 624 |
| Eco57I | CTGAAG | 1 cut(s) | 774 |
| Eco72I | CACGTG | 1 cut(s) | 458 |
| Eco88I | CYCGRG | 1 cut(s) | 462 |
| Eco91I | GGTNACC | 1 cut(s) | 228 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 289 |
| EcoO65I | GGTNACC | 1 cut(s) | 228 |
| EcoRI | GAATTC | 1 cut(s) | 826 |
| EcoRII | CCWGG | 3 cut(s) | 164, 232, 770 |
| EcoT14I | CCWWGG | 3 cut(s) | 194, 867, 976 |
| EcoT38I | GRGCYC | 1 cut(s) | 651 |
| ErhI | CCWWGG | 3 cut(s) | 194, 867, 976 |
| FaeI | CATG | 4 cut(s) | 198, 472, 624, 1073 |
| FaqI | GGGAC | 1 cut(s) | 313 |
| FatI | CATG | 4 cut(s) | 194, 468, 620, 1069 |
| FbaI | TGATCA | 1 cut(s) | 676 |
| Fnu4HI | GCNGC | 8 cut(s) | 141, 406, 473, 539, 542, 551, 570, 1026 |
| FokI | GGATG | 2 cut(s) | 791, 850 |
| FriOI | GRGCYC | 1 cut(s) | 651 |
| Fsp4HI | GCNGC | 8 cut(s) | 141, 406, 473, 539, 542, 551, 570, 1026 |
| FspBI | CTAG | 3 cut(s) | 107, 848, 1058 |
| GluI | GCNGC | 8 cut(s) | 141, 406, 473, 539, 542, 551, 570, 1026 |
| GsaI | CCCAGC | 1 cut(s) | 648 |
| HaeIII | GGCC | 3 cut(s) | 607, 776, 1043 |
| Hin1II | CATG | 4 cut(s) | 198, 472, 624, 1073 |
| HincII | GTYRAC | 1 cut(s) | 433 |
| HindII | GTYRAC | 1 cut(s) | 433 |
| HindIII | AAGCTT | 2 cut(s) | 707, 1016 |
| HinfI | GANTC | 6 cut(s) | 238, 371, 465, 857, 912, 1073 |
| HphI | GGTGA | 5 cut(s) | 222, 507, 760, 959, 992 |
| Hpy166II | GTNNAC | 1 cut(s) | 433 |
| Hpy188I | TCNGA | 9 cut(s) | 100, 311, 316, 350, 681, 754, 917, 1035, 1096 |
| Hpy188III | TCNNGA | 4 cut(s) | 56, 251, 493, 808 |
| Hpy8I | GTNNAC | 1 cut(s) | 433 |
| HpyAV | CCTTC | 2 cut(s) | 25, 285 |
| HpyCH4III | ACNGT | 4 cut(s) | 90, 427, 837, 947 |
| HpyCH4IV | ACGT | 1 cut(s) | 457 |
| HpyCH4V | TGCA | 6 cut(s) | 70, 541, 798, 863, 960, 1028 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 123, 547, 550, 1008 |
| HpyF3I | CTNAG | 4 cut(s) | 15, 575, 998, 1032 |
| HpySE526I | ACGT | 1 cut(s) | 457 |
| Hsp92II | CATG | 4 cut(s) | 198, 472, 624, 1073 |
| Ksp22I | TGATCA | 1 cut(s) | 676 |
| Kzo9I | GATC | 4 cut(s) | 311, 381, 676, 982 |
| LmnI | GCTCC | 3 cut(s) | 187, 210, 1066 |
| Lsp1109I | GCAGC | 6 cut(s) | 459, 525, 553, 556, 562, 1012 |
| LweI | GCATC | 3 cut(s) | 244, 392, 872 |
| MabI | ACCWGGT | 1 cut(s) | 164 |
| MaeI | CTAG | 3 cut(s) | 107, 848, 1058 |
| MaeII | ACGT | 1 cut(s) | 457 |
| MaeIII | GTNAC | 3 cut(s) | 228, 766, 967 |
| MalI | GATC | 4 cut(s) | 313, 383, 678, 984 |
| MboI | GATC | 4 cut(s) | 311, 381, 676, 982 |
| MboII | GAAGA | 4 cut(s) | 430, 504, 767, 1109 |
| MfeI | CAATTG | 2 cut(s) | 71, 784 |
| MhlI | GDGCHC | 3 cut(s) | 298, 463, 651 |
| MlyI | GAGTC | 2 cut(s) | 380, 474 |
| MnlI | CCTC | 9 cut(s) | 30, 146, 186, 195, 520, 638, 925, 958, 1074 |
| MseI | TTAA | 1 cut(s) | 444 |
| MspA1I | CMGCKG | 1 cut(s) | 143 |
| MspR9I | CCNGG | 3 cut(s) | 166, 234, 772 |
| MunI | CAATTG | 2 cut(s) | 71, 784 |
| MvaI | CCWGG | 3 cut(s) | 166, 234, 772 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 123, 547, 550, 1008 |
| NcoI | CCATGG | 1 cut(s) | 194 |
| NdeII | GATC | 4 cut(s) | 311, 381, 676, 982 |
| NlaIII | CATG | 4 cut(s) | 198, 472, 624, 1073 |
| NlaIV | GGNNCC | 2 cut(s) | 297, 626 |
| NmuCI | GTSAC | 2 cut(s) | 228, 766 |
| NspV | TTCGAA | 2 cut(s) | 289, 886 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 561 |
| PfeI | GAWTC | 4 cut(s) | 238, 857, 912, 1073 |
| PflFI | GACNNNGTC | 1 cut(s) | 764 |
| PkrI | GCNGC | 8 cut(s) | 142, 407, 474, 540, 543, 552, 571, 1027 |
| PleI | GAGTC | 2 cut(s) | 379, 473 |
| PmaCI | CACGTG | 1 cut(s) | 458 |
| PmlI | CACGTG | 1 cut(s) | 458 |
| PpsI | GAGTC | 2 cut(s) | 379, 473 |
| Ppu21I | YACGTR | 1 cut(s) | 458 |
| Psp6I | CCWGG | 3 cut(s) | 164, 232, 770 |
| PspCI | CACGTG | 1 cut(s) | 458 |
| PspEI | GGTNACC | 1 cut(s) | 228 |
| PspFI | CCCAGC | 1 cut(s) | 644 |
| PspGI | CCWGG | 3 cut(s) | 164, 232, 770 |
| PspN4I | GGNNCC | 2 cut(s) | 297, 626 |
| PspPI | GGNCC | 3 cut(s) | 624, 774, 1041 |
| PstI | CTGCAG | 1 cut(s) | 543 |
| PsyI | GACNNNGTC | 1 cut(s) | 764 |
| RsaI | GTAC | 5 cut(s) | 92, 263, 725, 732, 839 |
| RsaNI | GTAC | 5 cut(s) | 91, 262, 724, 731, 838 |
| SaqAI | TTAA | 1 cut(s) | 444 |
| SatI | GCNGC | 8 cut(s) | 141, 406, 473, 539, 542, 551, 570, 1026 |
| Sau3AI | GATC | 4 cut(s) | 311, 381, 676, 982 |
| Sau96I | GGNCC | 3 cut(s) | 624, 774, 1041 |
| ScaI | AGTACT | 1 cut(s) | 725 |
| SchI | GAGTC | 2 cut(s) | 380, 474 |
| ScrFI | CCNGG | 3 cut(s) | 166, 234, 772 |
| SduI | GDGCHC | 3 cut(s) | 298, 463, 651 |
| SexAI | ACCWGGT | 1 cut(s) | 164 |
| SfaNI | GCATC | 3 cut(s) | 244, 392, 872 |
| SfcI | CTRYAG | 1 cut(s) | 539 |
| SfuI | TTCGAA | 2 cut(s) | 289, 886 |
| SinI | GGWCC | 1 cut(s) | 624 |
| SsiI | CCGC | 2 cut(s) | 141, 406 |
| SspI | AATATT | 2 cut(s) | 245, 1113 |
| SspMI | CTAG | 3 cut(s) | 107, 848, 1058 |
| StyD4I | CCNGG | 3 cut(s) | 164, 232, 770 |
| StyI | CCWWGG | 3 cut(s) | 194, 867, 976 |
| TaaI | ACNGT | 4 cut(s) | 90, 427, 837, 947 |
| TaiI | ACGT | 1 cut(s) | 460 |
| TaqI | TCGA | 8 cut(s) | 252, 267, 289, 369, 651, 830, 886, 985 |
| TatI | WGTACW | 4 cut(s) | 90, 261, 723, 730 |
| TauI | GCSGC | 2 cut(s) | 143, 408 |
| TfiI | GAWTC | 4 cut(s) | 238, 857, 912, 1073 |
| Tru1I | TTAA | 1 cut(s) | 444 |
| Tru9I | TTAA | 1 cut(s) | 444 |
| TseFI | GTSAC | 2 cut(s) | 228, 766 |
| TseI | GCWGC | 6 cut(s) | 472, 538, 541, 550, 569, 1025 |
| Tsp45I | GTSAC | 2 cut(s) | 228, 766 |
| TspDTI | ATGAA | 6 cut(s) | 512, 597, 868, 1039, 1043, 1086 |
| Tth111I | GACNNNGTC | 1 cut(s) | 764 |
| VpaK11BI | GGWCC | 1 cut(s) | 624 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 289 |
| XapI | RAATTY | 3 cut(s) | 737, 811, 826 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 215 |
| XspI | CTAG | 3 cut(s) | 107, 848, 1058 |
| ZrmI | AGTACT | 1 cut(s) | 725 |