Rh3DG184800

Protein of unknown function (DUF295)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0000142
Physical Location & Seq
Reverse (-)
16392278 .. 16392664
387 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG184800.1

Sequence Viewer

Length: 387 bp
ATGGAAAAAATTCTTAAGATTATAAGTAAATCTGCATGGCATCTGTTGATCATGACTGAATTTTTGATGAAACGTCCTAATTGGTCTCAGAATTTTTTCATCAATCCAATGACAGGGGCTTGGCTTATGCTGCCACCACTGGTGAGCGCCTGCCCATGGATCTCCCTGGCCTCTGCAGATCCAAAATGTTCGAAATGCATTGTGGCACCAATTACATATAACAGAAGTAGGTTGGTGTTTTGCAGGCCACCTGATGAATCGTGGACTTTCCAACAGATGAAAGATGATGAACACATGGCTGAAATTAAATTCCAGGATGGGAAGCTATACGCAATGGGACGGGAGTACGTAGTTGTTTCTGATCCCTTAGAAGTTCTTGATCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box F-box-like

Protein Analysis

128

Amino Acids

14.86

Weight (kDa)

6.71

Isoelectric Point (pI)

36.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 16 - 116 3.6e-11 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000142)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g13532 FvH4_2g28870 FvH4_3g30930 FvH4_3g37281 FvH4_3g37300 FvH4_3g37301 FvH4_3g37301 FvH4_4g23711 FvH4_5g02330 FvH4_6g16960 FvH4_6g16971 FvH4_6g21291 FvH4_6g21331 FvH4_6g22212 FvH4_6g22332 FvH4_6g36920 FvH4_6g37552 FvH4_6g37591 FvH4_6g39721 FvH4_6g46020 FvH4_6g47001 FvH4_6g47002 FvH4_6g47130 FvH4_6g47140 FvH4_6g47161 FvH4_6g47170 FvH4_6g47190 FvH4_6g49120 FvH4_6g49280 FvH4_6g49281 FvH4_6g49640 FvH4_6g49640
malus_domestica MD03G1085600.v1.1 MD09G1027500.v1.1 MD09G1050100.v1.1
prunus_persica Prupe.1G004300_v2.0.a1 Prupe.1G016300_v2.0.a1 Prupe.1G019300_v2.0.a1 Prupe.3G239100_v2.0.a1 Prupe.3G291200_v2.0.a1
pyrus_communis pycom08g02840 pycom08g02860 pycom08g02870
rosa_chinensis RchiOBHm_Chr2g0153831 RchiOBHm_Chr2g0166171 RchiOBHm_Chr2g0166201 RchiOBHm_Chr3g0476221 RchiOBHm_Chr4g0430971 RchiOBHm_Chr5g0067081 RchiOBHm_Chr7g0197821
rosa_laevigata RLG00000001284 RLG00000006996 RLG00000012710 RLG00000019299 RLG00000020285 RLG00000020444 RLG00000020466 RLG00000020591 RLG00000020665 RLG00000020730 RLG00000020732 RLG00000021449 RLG00000021592 RLG00000021593 RLG00000021594 RLG00000021596 RLG00000021597 RLG00000021801 RLG00000023534 RLG00000023538 RLG00000023693 RLG00000023694 RLG00000023699 RLG00000024196 RLG00000024214 RLG00000024220 RLG00000024244 RLG00000024608 RLG00000024612 RLG00000035157 RLG00000035906
rosa_multiflora Rmu_co8282815.1_g000001 Rmu_co8380495.1_g000001 Rmu_co8381111.1_g000001 Rmu_co8460513.1_g000001 Rmu_co8481947.1_g000001 Rmu_co8487937.1_g000001 Rmu_sc0000151.1_g000017 Rmu_sc0001427.1_g000003 Rmu_sc0001427.1_g000006 Rmu_sc0002611.1_g000008 Rmu_sc0003887.1_g000018 Rmu_sc0003887.1_g000019 Rmu_sc0003887.1_g000021 Rmu_sc0004119.1_g000004 Rmu_sc0004329.1_g000003 Rmu_sc0004485.1_g000024 Rmu_sc0005290.1_g000001 Rmu_sc0005609.1_g000006 Rmu_sc0006187.1_g000018 Rmu_sc0006218.1_g000032 Rmu_sc0006218.1_g000033 Rmu_sc0007150.1_g000003 Rmu_sc0007154.1_g000004 Rmu_sc0007154.1_g000005 Rmu_sc0007154.1_g000007 Rmu_sc0008314.1_g000012 Rmu_sc0010073.1_g000023 Rmu_sc0010073.1_g000025 Rmu_sc0011216.1_g000008 Rmu_sc0012445.1_g000005 Rmu_sc0013571.1_g000002 Rmu_sc0018648.1_g000002 Rmu_sc0020961.1_g000002 Rmu_sc0020961.1_g000003 Rmu_sc0023710.1_g000001 Rmu_sc0031310.1_g000008 Rmu_sc0033940.1_g000001 Rmu_sc0035493.1_g000001 Rmu_sc0039285.1_g000001
rosa_roxburghii Rroxscaffold_1G00014000 Rroxscaffold_1G00014020 Rroxscaffold_2G00082760 Rroxscaffold_2G00085090 Rroxscaffold_2G00085100 Rroxscaffold_2G00085140 Rroxscaffold_2G00086670 Rroxscaffold_2G00086680 Rroxscaffold_2G00098190 Rroxscaffold_2G00099270 Rroxscaffold_3G00227460 Rroxscaffold_3G00258190 Rroxscaffold_3G00259780 Rroxscaffold_6G00402940 Rroxscaffold_6G00410200 Rroxscaffold_6G00410210 Rroxscaffold_7G00184320
rosa_rugosa Rorug02G0416100 Rorug02G0416200 Rorug02G0520900 Rorug02G0521200 Rorug02G0521300 Rorug02G0534600 Rorug03G0116300 Rorug03G0162900 Rorug03G0162900 Rorug04G0241400 Rorug05G0380900 Rorug07G0043800
rosa_samantha Rh1AG115300 Rh2BG483700 Rh2BG509900 Rh2BG515700 Rh2BG515800 Rh2BG586500 Rh2BG586600 Rh2BG586700 Rh2BG598800 Rh2BG598900 Rh2BG599100 Rh2BG618400 Rh2BG630500 Rh2CG457400 Rh2CG484900 Rh3AG165300 Rh3AG212900 Rh3AG213300 Rh3BG145800 Rh3BG146100 Rh3BG235300 Rh3BG246500 Rh3BG247000 Rh3BG247100 Rh3CG240300 Rh3CG240800 Rh3CG240900 Rh3CG259600 Rh3DG147000 Rh3DG184800 Rh3DG184900 Rh3DG185000 Rh3DG239500 Rh3DG240100 Rh3DG240200 Rh3DG255800 Rh5BG458500 Rh5CG480400 Rh7BG170400 Rh7BG407900 Rh7CG176900
rosa_wichuraiana Rw2G038990 Rw2G041550 Rw2G047660 Rw2G048940 Rw4G026370 Rw6G008730 Rw7G014600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 23
AccB1I GGYRCC 1 cut(s) 205
AclWI GGATC 3 cut(s) 167, 173, 356
AcsI RAATTY 4 cut(s) 9, 59, 91, 308
AfaI GTAC 1 cut(s) 347
AfiI CCNNNNNNNGG 2 cut(s) 113, 156
AflII CTTAAG 1 cut(s) 14
AjnI CCWGG 2 cut(s) 165, 312
AluBI AGCT 1 cut(s) 325
AluI AGCT 1 cut(s) 325
Alw26I GTCTC 1 cut(s) 90
AlwI GGATC 3 cut(s) 167, 173, 356
AoxI GGCC 2 cut(s) 168, 245
ApeKI GCWGC 1 cut(s) 130
ApoI RAATTY 4 cut(s) 9, 59, 91, 308
ArsI GACNNNNNNTTYG 2 cut(s) 46, 78
Asp700I GAANNNNTTC 2 cut(s) 9, 95
AspLEI GCGC 1 cut(s) 149
AsuHPI GGTGA 1 cut(s) 154
AsuII TTCGAA 1 cut(s) 191
BanI GGYRCC 1 cut(s) 205
BbvI GCAGC 1 cut(s) 117
BccI CCATC 1 cut(s) 311
BciT130I CCWGG 2 cut(s) 167, 314
BclI TGATCA 2 cut(s) 48, 379
BcoDI GTCTC 1 cut(s) 90
BfmI CTRYAG 1 cut(s) 174
BfoI RGCGCY 1 cut(s) 150
BfrI CTTAAG 1 cut(s) 14
BisI GCNGC 1 cut(s) 131
BlsI GCNGC 1 cut(s) 132
Bme1390I CCNGG 2 cut(s) 167, 314
BmiI GGNNCC 1 cut(s) 207
BmrFI CCNGG 2 cut(s) 167, 314
BmsI GCATC 1 cut(s) 49
Bpu14I TTCGAA 1 cut(s) 191
BsaAI YACGTR 1 cut(s) 349
BsaI GGTCTC 1 cut(s) 90
BsaJI CCNNGG 2 cut(s) 155, 165
Bsc4I CCNNNNNNNGG 2 cut(s) 113, 156
Bse1I ACTGG 1 cut(s) 144
Bse3DI GCAATG 1 cut(s) 339
BseBI CCWGG 2 cut(s) 167, 314
BseDI CCNNGG 2 cut(s) 155, 165
BseGI GGATG 1 cut(s) 322
BseLI CCNNNNNNNGG 2 cut(s) 113, 156
BseMI GCAATG 1 cut(s) 339
BseMII CTCAG 1 cut(s) 101
BseNI ACTGG 1 cut(s) 144
BseXI GCAGC 1 cut(s) 117
BshFI GGCC 2 cut(s) 170, 247
BshNI GGYRCC 1 cut(s) 205
BslFI GGGAC 1 cut(s) 351
BslI CCNNNNNNNGG 2 cut(s) 113, 156
BsmAI GTCTC 1 cut(s) 90
BsmFI GGGAC 1 cut(s) 351
BsnI GGCC 2 cut(s) 170, 247
Bso31I GGTCTC 1 cut(s) 90
Bsp119I TTCGAA 1 cut(s) 191
Bsp143I GATC 5 cut(s) 48, 159, 178, 361, 379
Bsp19I CCATGG 1 cut(s) 155
BspANI GGCC 2 cut(s) 170, 247
BspCNI CTCAG 1 cut(s) 100
BspHI TCATGA 1 cut(s) 51
BspLI GGNNCC 1 cut(s) 207
BspMAI CTGCAG 1 cut(s) 178
BspPI GGATC 3 cut(s) 167, 173, 356
BspT104I TTCGAA 1 cut(s) 191
BspT107I GGYRCC 1 cut(s) 205
BspTI CTTAAG 1 cut(s) 14
BspTNI GGTCTC 1 cut(s) 90
BsrDI GCAATG 1 cut(s) 339
BsrI ACTGG 1 cut(s) 144
BssECI CCNNGG 2 cut(s) 155, 165
BssMI GATC 5 cut(s) 48, 159, 178, 361, 379
BssT1I CCWWGG 1 cut(s) 155
Bst2UI CCWGG 2 cut(s) 167, 314
BstAFI CTTAAG 1 cut(s) 14
BstBAI YACGTR 1 cut(s) 349
BstBI TTCGAA 1 cut(s) 191
BstC8I GCNNGC 2 cut(s) 151, 245
BstDEI CTNAG 2 cut(s) 87, 367
BstDSI CCRYGG 1 cut(s) 155
BstF5I GGATG 1 cut(s) 322
BstH2I RGCGCY 1 cut(s) 150
BstHHI GCGC 1 cut(s) 149
BstKTI GATC 5 cut(s) 51, 162, 181, 364, 382
BstMAI GTCTC 1 cut(s) 90
BstMBI GATC 5 cut(s) 48, 159, 178, 361, 379
BstMWI GCNNNNNNNGC 1 cut(s) 130
BstNI CCWGG 2 cut(s) 167, 314
BstSCI CCNGG 2 cut(s) 165, 312
BstSFI CTRYAG 1 cut(s) 174
BstSNI TACGTA 1 cut(s) 349
BstV1I GCAGC 1 cut(s) 117
BstX2I RGATCY 2 cut(s) 159, 178
BstYI RGATCY 2 cut(s) 159, 178
BsuRI GGCC 2 cut(s) 170, 247
BtgI CCRYGG 1 cut(s) 155
BtsCI GGATG 1 cut(s) 322
BtsIMutI CAGTG 1 cut(s) 137
Cac8I GCNNGC 2 cut(s) 151, 245
CciI TCATGA 1 cut(s) 51
CfoI GCGC 1 cut(s) 149
Csp6I GTAC 1 cut(s) 346
CviAII CATG 4 cut(s) 36, 52, 156, 295
CviJI RGCY 6 cut(s) 119, 124, 170, 247, 299, 325
CviKI_1 RGCY 6 cut(s) 119, 124, 170, 247, 299, 325
CviQI GTAC 1 cut(s) 346
DdeI CTNAG 2 cut(s) 87, 367
DpnI GATC 5 cut(s) 50, 161, 180, 363, 381
DpnII GATC 5 cut(s) 48, 159, 178, 361, 379
Eco105I TACGTA 1 cut(s) 349
Eco130I CCWWGG 1 cut(s) 155
Eco31I GGTCTC 1 cut(s) 90
EcoRII CCWGG 2 cut(s) 165, 312
EcoT14I CCWWGG 1 cut(s) 155
EcoT22I ATGCAT 1 cut(s) 200
ErhI CCWWGG 1 cut(s) 155
FaeI CATG 4 cut(s) 39, 55, 159, 298
FaiI YATR 9 cut(s) 23, 37, 53, 128, 157, 217, 219, 296, 328
FaqI GGGAC 1 cut(s) 351
FatI CATG 4 cut(s) 35, 51, 155, 294
FbaI TGATCA 2 cut(s) 48, 379
Fnu4HI GCNGC 1 cut(s) 131
FokI GGATG 1 cut(s) 329
Fsp4HI GCNGC 1 cut(s) 131
GlaI GCGC 1 cut(s) 148
GluI GCNGC 1 cut(s) 131
HaeII RGCGCY 1 cut(s) 150
HaeIII GGCC 2 cut(s) 170, 247
HhaI GCGC 1 cut(s) 149
Hin1II CATG 4 cut(s) 39, 55, 159, 298
Hin6I GCGC 1 cut(s) 147
HinP1I GCGC 1 cut(s) 147
HinfI GANTC 1 cut(s) 257
HphI GGTGA 1 cut(s) 154
Hpy166II GTNNAC 1 cut(s) 264
Hpy188I TCNGA 2 cut(s) 90, 361
Hpy188III TCNNGA 2 cut(s) 52, 377
Hpy8I GTNNAC 1 cut(s) 264
HpyCH4IV ACGT 2 cut(s) 73, 348
HpyCH4V TGCA 4 cut(s) 35, 176, 198, 243
HpyF10VI GCNNNNNNNGC 1 cut(s) 130
HpyF3I CTNAG 2 cut(s) 87, 367
HpySE526I ACGT 2 cut(s) 73, 348
Hsp92II CATG 4 cut(s) 39, 55, 159, 298
HspAI GCGC 1 cut(s) 147
Ksp22I TGATCA 2 cut(s) 48, 379
Kzo9I GATC 5 cut(s) 48, 159, 178, 361, 379
LpnPI CCDG 9 cut(s) 99, 125, 152, 163, 179, 229, 264, 299, 326
Lsp1109I GCAGC 1 cut(s) 117
LweI GCATC 1 cut(s) 49
MaeII ACGT 2 cut(s) 73, 348
MalI GATC 5 cut(s) 50, 161, 180, 363, 381
MboI GATC 5 cut(s) 48, 159, 178, 361, 379
MflI RGATCY 2 cut(s) 159, 178
MluCI AATT 7 cut(s) 9, 59, 79, 91, 210, 303, 308
MmeI TCCRAC 1 cut(s) 295
MnlI CCTC 1 cut(s) 181
Mph1103I ATGCAT 1 cut(s) 200
MroXI GAANNNNTTC 2 cut(s) 9, 95
MseI TTAA 2 cut(s) 15, 306
MspCI CTTAAG 1 cut(s) 14
MspR9I CCNGG 2 cut(s) 167, 314
MvaI CCWGG 2 cut(s) 167, 314
MwoI GCNNNNNNNGC 1 cut(s) 130
NcoI CCATGG 1 cut(s) 155
NdeII GATC 5 cut(s) 48, 159, 178, 361, 379
NlaIII CATG 4 cut(s) 39, 55, 159, 298
NlaIV GGNNCC 1 cut(s) 207
NsiI ATGCAT 1 cut(s) 200
NspV TTCGAA 1 cut(s) 191
PagI TCATGA 1 cut(s) 51
PdmI GAANNNNTTC 2 cut(s) 9, 95
PfeI GAWTC 1 cut(s) 257
PfoI TCCNGGA 1 cut(s) 312
PkrI GCNGC 1 cut(s) 132
Ppu21I YACGTR 1 cut(s) 349
PsiI TTATAA 1 cut(s) 23
Psp6I CCWGG 2 cut(s) 165, 312
PspGI CCWGG 2 cut(s) 165, 312
PspN4I GGNNCC 1 cut(s) 207
PstI CTGCAG 1 cut(s) 178
PsuI RGATCY 2 cut(s) 159, 178
RsaI GTAC 1 cut(s) 347
RsaNI GTAC 1 cut(s) 346
SaqAI TTAA 2 cut(s) 15, 306
SatI GCNGC 1 cut(s) 131
Sau3AI GATC 5 cut(s) 48, 159, 178, 361, 379
ScrFI CCNGG 2 cut(s) 167, 314
SetI ASST 5 cut(s) 76, 233, 253, 327, 351
SfaNI GCATC 1 cut(s) 49
SfcI CTRYAG 1 cut(s) 174
SfuI TTCGAA 1 cut(s) 191
SmlI CTYRAG 1 cut(s) 14
SmoI CTYRAG 1 cut(s) 14
SnaBI TACGTA 1 cut(s) 349
Sse9I AATT 7 cut(s) 9, 59, 79, 91, 210, 303, 308
StyD4I CCNGG 2 cut(s) 165, 312
StyI CCWWGG 1 cut(s) 155
TaiI ACGT 2 cut(s) 76, 351
TaqI TCGA 1 cut(s) 191
TasI AATT 7 cut(s) 9, 59, 79, 91, 210, 303, 308
TfiI GAWTC 1 cut(s) 257
Tru1I TTAA 2 cut(s) 15, 306
Tru9I TTAA 2 cut(s) 15, 306
TscAI CASTG 1 cut(s) 144
TseI GCWGC 1 cut(s) 130
TspDTI ATGAA 5 cut(s) 83, 88, 270, 293, 303
TspRI CASTG 1 cut(s) 144
Vha464I CTTAAG 1 cut(s) 14
XapI RAATTY 4 cut(s) 9, 59, 91, 308
XmnI GAANNNNTTC 2 cut(s) 9, 95
Zsp2I ATGCAT 1 cut(s) 200
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.