Ubiquitin carboxyl-terminal hydrolase
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 888 |
| AarI | CACCTGC | 1 cut(s) | 690 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 938 |
| Acc36I | ACCTGC | 3 cut(s) | 690, 1149, 1240 |
| AccB1I | GGYRCC | 1 cut(s) | 561 |
| AccI | GTMKAC | 2 cut(s) | 237, 643 |
| AccII | CGCG | 1 cut(s) | 770 |
| AciI | CCGC | 3 cut(s) | 453, 768, 1029 |
| AclWI | GGATC | 5 cut(s) | 461, 665, 746, 759, 1043 |
| AcsI | RAATTY | 5 cut(s) | 434, 511, 590, 1033, 1512 |
| AcuI | CTGAAG | 5 cut(s) | 264, 447, 948, 1239, 1301 |
| AfaI | GTAC | 6 cut(s) | 579, 758, 1146, 1163, 1313, 1382 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 401, 557 |
| AflIII | ACRYGT | 1 cut(s) | 1452 |
| AgsI | TTSAA | 6 cut(s) | 50, 84, 349, 391, 1078, 1450 |
| AhlI | ACTAGT | 1 cut(s) | 1067 |
| AjnI | CCWGG | 3 cut(s) | 745, 776, 1383 |
| AluBI | AGCT | 4 cut(s) | 418, 1217, 1436, 1484 |
| AluI | AGCT | 4 cut(s) | 418, 1217, 1436, 1484 |
| Alw21I | GWGCWC | 1 cut(s) | 1411 |
| Alw26I | GTCTC | 2 cut(s) | 141, 786 |
| AlwI | GGATC | 5 cut(s) | 461, 665, 746, 759, 1043 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 197, 1384 |
| Ama87I | CYCGRG | 1 cut(s) | 1307 |
| AoxI | GGCC | 1 cut(s) | 527 |
| ApeKI | GCWGC | 3 cut(s) | 95, 418, 455 |
| ApoI | RAATTY | 5 cut(s) | 434, 511, 590, 1033, 1512 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 968, 1000 |
| AseI | ATTAAT | 1 cut(s) | 315 |
| AspLEI | GCGC | 1 cut(s) | 772 |
| AspS9I | GGNCC | 3 cut(s) | 21, 798, 1252 |
| AsuHPI | GGTGA | 5 cut(s) | 416, 641, 821, 913, 1217 |
| AsuII | TTCGAA | 1 cut(s) | 856 |
| AvaI | CYCGRG | 1 cut(s) | 1307 |
| AvaII | GGWCC | 3 cut(s) | 21, 798, 1252 |
| BaeGI | GKGCMC | 1 cut(s) | 564 |
| BamHI | GGATCC | 1 cut(s) | 751 |
| BanI | GGYRCC | 1 cut(s) | 561 |
| BanII | GRGCYC | 1 cut(s) | 311 |
| BbsI | GAAGAC | 2 cut(s) | 234, 936 |
| Bbv12I | GWGCWC | 1 cut(s) | 1411 |
| BbvI | GCAGC | 3 cut(s) | 82, 405, 467 |
| BccI | CCATC | 4 cut(s) | 383, 596, 793, 1087 |
| BciT130I | CCWGG | 3 cut(s) | 747, 778, 1385 |
| BciVI | GTATCC | 2 cut(s) | 288, 1227 |
| BcoDI | GTCTC | 2 cut(s) | 141, 786 |
| BcuI | ACTAGT | 1 cut(s) | 1067 |
| BfaI | CTAG | 4 cut(s) | 74, 185, 1068, 1104 |
| BfmI | CTRYAG | 1 cut(s) | 214 |
| BfuAI | ACCTGC | 3 cut(s) | 690, 1149, 1240 |
| BfuI | GTATCC | 2 cut(s) | 288, 1227 |
| BisI | GCNGC | 3 cut(s) | 96, 419, 456 |
| BlsI | GCNGC | 3 cut(s) | 97, 420, 457 |
| Bme1390I | CCNGG | 3 cut(s) | 747, 778, 1385 |
| Bme18I | GGWCC | 3 cut(s) | 21, 798, 1252 |
| BmeT110I | CYCGRG | 1 cut(s) | 1307 |
| BmgT120I | GGNCC | 3 cut(s) | 21, 798, 1252 |
| BmiI | GGNNCC | 4 cut(s) | 563, 753, 1179, 1254 |
| BmrFI | CCNGG | 3 cut(s) | 747, 778, 1385 |
| BmsI | GCATC | 3 cut(s) | 37, 265, 1140 |
| BpiI | GAAGAC | 2 cut(s) | 234, 936 |
| Bpu14I | TTCGAA | 1 cut(s) | 856 |
| BpuEI | CTTGAG | 1 cut(s) | 230 |
| BsaAI | YACGTR | 1 cut(s) | 616 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1053 |
| BsaI | GGTCTC | 2 cut(s) | 141, 786 |
| BsaJI | CCNNGG | 4 cut(s) | 158, 551, 746, 1384 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 401, 557 |
| Bse1I | ACTGG | 1 cut(s) | 1338 |
| Bse3DI | GCAATG | 1 cut(s) | 1024 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1053 |
| BseBI | CCWGG | 3 cut(s) | 747, 778, 1385 |
| BseDI | CCNNGG | 4 cut(s) | 158, 551, 746, 1384 |
| BseGI | GGATG | 6 cut(s) | 10, 280, 438, 449, 607, 1098 |
| BseJI | GATNNNNATC | 1 cut(s) | 1053 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 401, 557 |
| BseMI | GCAATG | 1 cut(s) | 1024 |
| BseMII | CTCAG | 2 cut(s) | 168, 204 |
| BseNI | ACTGG | 1 cut(s) | 1338 |
| BseSI | GKGCMC | 1 cut(s) | 564 |
| BseXI | GCAGC | 3 cut(s) | 82, 405, 467 |
| BseYI | CCCAGC | 1 cut(s) | 423 |
| Bsh1236I | CGCG | 1 cut(s) | 770 |
| Bsh1285I | CGRYCG | 1 cut(s) | 1057 |
| BshFI | GGCC | 1 cut(s) | 529 |
| BshNI | GGYRCC | 1 cut(s) | 561 |
| BsiEI | CGRYCG | 1 cut(s) | 1057 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1411 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1307 |
| BslFI | GGGAC | 2 cut(s) | 1238, 1290 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 401, 557 |
| BsmAI | GTCTC | 2 cut(s) | 141, 786 |
| BsmFI | GGGAC | 2 cut(s) | 1238, 1290 |
| BsmI | GAATGC | 1 cut(s) | 1286 |
| BsnI | GGCC | 1 cut(s) | 529 |
| Bso31I | GGTCTC | 2 cut(s) | 141, 786 |
| BsoBI | CYCGRG | 1 cut(s) | 1307 |
| Bsp119I | TTCGAA | 1 cut(s) | 856 |
| Bsp1286I | GDGCHC | 3 cut(s) | 311, 564, 1411 |
| Bsp1407I | TGTACA | 2 cut(s) | 577, 1144 |
| Bsp143I | GATC | 6 cut(s) | 406, 466, 670, 751, 1048, 1054 |
| Bsp19I | CCATGG | 1 cut(s) | 158 |
| BspACI | CCGC | 3 cut(s) | 453, 768, 1029 |
| BspANI | GGCC | 1 cut(s) | 529 |
| BspCNI | CTCAG | 2 cut(s) | 169, 203 |
| BspFNI | CGCG | 1 cut(s) | 770 |
| BspHI | TCATGA | 3 cut(s) | 538, 595, 1198 |
| BspLI | GGNNCC | 4 cut(s) | 563, 753, 1179, 1254 |
| BspMI | ACCTGC | 3 cut(s) | 690, 1149, 1240 |
| BspPI | GGATC | 5 cut(s) | 461, 665, 746, 759, 1043 |
| BspT104I | TTCGAA | 1 cut(s) | 856 |
| BspT107I | GGYRCC | 1 cut(s) | 561 |
| BspTNI | GGTCTC | 2 cut(s) | 141, 786 |
| BsrDI | GCAATG | 1 cut(s) | 1024 |
| BsrGI | TGTACA | 2 cut(s) | 577, 1144 |
| BsrI | ACTGG | 1 cut(s) | 1338 |
| BssECI | CCNNGG | 4 cut(s) | 158, 551, 746, 1384 |
| BssMI | GATC | 6 cut(s) | 406, 466, 670, 751, 1048, 1054 |
| BssNAI | GTATAC | 1 cut(s) | 644 |
| BssT1I | CCWWGG | 2 cut(s) | 158, 551 |
| Bst1107I | GTATAC | 1 cut(s) | 644 |
| Bst2UI | CCWGG | 3 cut(s) | 747, 778, 1385 |
| Bst4CI | ACNGT | 2 cut(s) | 199, 218 |
| Bst6I | CTCTTC | 2 cut(s) | 466, 1257 |
| BstAUI | TGTACA | 2 cut(s) | 577, 1144 |
| BstBAI | YACGTR | 1 cut(s) | 616 |
| BstBI | TTCGAA | 1 cut(s) | 856 |
| BstC8I | GCNNGC | 1 cut(s) | 527 |
| BstDEI | CTNAG | 4 cut(s) | 177, 190, 1127, 1413 |
| BstDSI | CCRYGG | 1 cut(s) | 158 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 555 |
| BstF5I | GGATG | 6 cut(s) | 10, 280, 438, 449, 607, 1098 |
| BstFNI | CGCG | 1 cut(s) | 770 |
| BstHHI | GCGC | 1 cut(s) | 772 |
| BstKTI | GATC | 6 cut(s) | 409, 469, 673, 754, 1051, 1057 |
| BstMAI | GTCTC | 2 cut(s) | 141, 786 |
| BstMBI | GATC | 6 cut(s) | 406, 466, 670, 751, 1048, 1054 |
| BstMCI | CGRYCG | 1 cut(s) | 1057 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 424, 1406 |
| BstNI | CCWGG | 3 cut(s) | 747, 778, 1385 |
| BstNSI | RCATGY | 1 cut(s) | 1456 |
| BstSCI | CCNGG | 3 cut(s) | 745, 776, 1383 |
| BstSFI | CTRYAG | 1 cut(s) | 214 |
| BstSLI | GKGCMC | 1 cut(s) | 564 |
| BstSNI | TACGTA | 1 cut(s) | 616 |
| BstUI | CGCG | 1 cut(s) | 770 |
| BstV1I | GCAGC | 3 cut(s) | 82, 405, 467 |
| BstV2I | GAAGAC | 2 cut(s) | 234, 936 |
| BstX2I | RGATCY | 3 cut(s) | 466, 670, 751 |
| BstYI | RGATCY | 3 cut(s) | 466, 670, 751 |
| BstZ17I | GTATAC | 1 cut(s) | 644 |
| BsuI | GTATCC | 2 cut(s) | 288, 1227 |
| BsuRI | GGCC | 1 cut(s) | 529 |
| BtgI | CCRYGG | 1 cut(s) | 158 |
| BtsCI | GGATG | 6 cut(s) | 10, 280, 438, 449, 607, 1098 |
| BveI | ACCTGC | 3 cut(s) | 690, 1149, 1240 |
| Cac8I | GCNNGC | 1 cut(s) | 527 |
| CaiI | CAGNNNCTG | 2 cut(s) | 197, 1384 |
| CciI | TCATGA | 3 cut(s) | 538, 595, 1198 |
| CfoI | GCGC | 1 cut(s) | 772 |
| Cfr13I | GGNCC | 3 cut(s) | 21, 798, 1252 |
| CseI | GACGC | 1 cut(s) | 53 |
| Csp6I | GTAC | 6 cut(s) | 578, 757, 1145, 1162, 1312, 1381 |
| CviAII | CATG | 9 cut(s) | 159, 539, 575, 596, 1199, 1230, 1422, 1453, 1461 |
| CviQI | GTAC | 6 cut(s) | 578, 757, 1145, 1162, 1312, 1381 |
| DdeI | CTNAG | 4 cut(s) | 177, 190, 1127, 1413 |
| DpnI | GATC | 6 cut(s) | 408, 468, 672, 753, 1050, 1056 |
| DpnII | GATC | 6 cut(s) | 406, 466, 670, 751, 1048, 1054 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 938 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 938 |
| Eam1104I | CTCTTC | 2 cut(s) | 466, 1257 |
| EarI | CTCTTC | 2 cut(s) | 466, 1257 |
| Eco105I | TACGTA | 1 cut(s) | 616 |
| Eco130I | CCWWGG | 2 cut(s) | 158, 551 |
| Eco24I | GRGCYC | 1 cut(s) | 311 |
| Eco31I | GGTCTC | 2 cut(s) | 141, 786 |
| Eco47I | GGWCC | 3 cut(s) | 21, 798, 1252 |
| Eco57I | CTGAAG | 5 cut(s) | 264, 447, 948, 1239, 1301 |
| Eco88I | CYCGRG | 1 cut(s) | 1307 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 555 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1252 |
| EcoRI | GAATTC | 1 cut(s) | 434 |
| EcoRII | CCWGG | 3 cut(s) | 745, 776, 1383 |
| EcoT14I | CCWWGG | 2 cut(s) | 158, 551 |
| EcoT22I | ATGCAT | 1 cut(s) | 1462 |
| EcoT38I | GRGCYC | 1 cut(s) | 311 |
| ErhI | CCWWGG | 2 cut(s) | 158, 551 |
| FaeI | CATG | 9 cut(s) | 162, 542, 578, 599, 1202, 1233, 1425, 1456, 1464 |
| FaqI | GGGAC | 2 cut(s) | 1238, 1290 |
| FatI | CATG | 9 cut(s) | 158, 538, 574, 595, 1198, 1229, 1421, 1452, 1460 |
| FblI | GTMKAC | 2 cut(s) | 237, 643 |
| Fnu4HI | GCNGC | 3 cut(s) | 96, 419, 456 |
| FokI | GGATG | 6 cut(s) | 17, 287, 425, 436, 614, 1105 |
| FriOI | GRGCYC | 1 cut(s) | 311 |
| Fsp4HI | GCNGC | 3 cut(s) | 96, 419, 456 |
| FspBI | CTAG | 4 cut(s) | 74, 185, 1068, 1104 |
| GlaI | GCGC | 1 cut(s) | 771 |
| GluI | GCNGC | 3 cut(s) | 96, 419, 456 |
| GsaI | CCCAGC | 1 cut(s) | 427 |
| HaeIII | GGCC | 1 cut(s) | 529 |
| HgaI | GACGC | 1 cut(s) | 53 |
| HhaI | GCGC | 1 cut(s) | 772 |
| Hin1II | CATG | 9 cut(s) | 162, 542, 578, 599, 1202, 1233, 1425, 1456, 1464 |
| Hin6I | GCGC | 1 cut(s) | 770 |
| HinP1I | GCGC | 1 cut(s) | 770 |
| HincII | GTYRAC | 1 cut(s) | 1065 |
| HindII | GTYRAC | 1 cut(s) | 1065 |
| HinfI | GANTC | 7 cut(s) | 80, 90, 170, 181, 188, 234, 1322 |
| HphI | GGTGA | 5 cut(s) | 416, 641, 821, 913, 1217 |
| Hpy166II | GTNNAC | 3 cut(s) | 238, 644, 1065 |
| Hpy188I | TCNGA | 9 cut(s) | 89, 193, 274, 283, 337, 466, 481, 1327, 1350 |
| Hpy8I | GTNNAC | 3 cut(s) | 238, 644, 1065 |
| Hpy99I | CGWCG | 1 cut(s) | 1049 |
| HpyAV | CCTTC | 5 cut(s) | 117, 497, 551, 1196, 1325 |
| HpyCH4III | ACNGT | 2 cut(s) | 199, 218 |
| HpyCH4IV | ACGT | 3 cut(s) | 298, 615, 635 |
| HpyCH4V | TGCA | 7 cut(s) | 486, 494, 525, 1249, 1286, 1425, 1460 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 424, 1406 |
| HpyF3I | CTNAG | 4 cut(s) | 177, 190, 1127, 1413 |
| HpySE526I | ACGT | 3 cut(s) | 298, 615, 635 |
| Hsp92II | CATG | 9 cut(s) | 162, 542, 578, 599, 1202, 1233, 1425, 1456, 1464 |
| HspAI | GCGC | 1 cut(s) | 770 |
| Kzo9I | GATC | 6 cut(s) | 406, 466, 670, 751, 1048, 1054 |
| LmnI | GCTCC | 2 cut(s) | 1375, 1414 |
| Lsp1109I | GCAGC | 3 cut(s) | 82, 405, 467 |
| LweI | GCATC | 3 cut(s) | 37, 265, 1140 |
| MaeI | CTAG | 4 cut(s) | 74, 185, 1068, 1104 |
| MaeII | ACGT | 3 cut(s) | 298, 615, 635 |
| MaeIII | GTNAC | 1 cut(s) | 145 |
| MalI | GATC | 6 cut(s) | 408, 468, 672, 753, 1050, 1056 |
| MboI | GATC | 6 cut(s) | 406, 466, 670, 751, 1048, 1054 |
| MboII | GAAGA | 8 cut(s) | 23, 239, 453, 925, 941, 1126, 1177, 1274 |
| MfeI | CAATTG | 1 cut(s) | 495 |
| MflI | RGATCY | 3 cut(s) | 466, 670, 751 |
| MhlI | GDGCHC | 3 cut(s) | 311, 564, 1411 |
| MlyI | GAGTC | 3 cut(s) | 182, 243, 1316 |
| MmeI | TCCRAC | 2 cut(s) | 252, 1489 |
| MnlI | CCTC | 8 cut(s) | 254, 469, 592, 811, 822, 833, 976, 1000 |
| Mph1103I | ATGCAT | 1 cut(s) | 1462 |
| MseI | TTAA | 4 cut(s) | 315, 414, 588, 677 |
| MslI | CAYNNNNRTG | 5 cut(s) | 130, 600, 968, 1112, 1203 |
| MspR9I | CCNGG | 3 cut(s) | 747, 778, 1385 |
| MunI | CAATTG | 1 cut(s) | 495 |
| Mva1269I | GAATGC | 1 cut(s) | 1286 |
| MvaI | CCWGG | 3 cut(s) | 747, 778, 1385 |
| MvnI | CGCG | 1 cut(s) | 770 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 424, 1406 |
| NcoI | CCATGG | 1 cut(s) | 158 |
| NdeII | GATC | 6 cut(s) | 406, 466, 670, 751, 1048, 1054 |
| NlaIII | CATG | 9 cut(s) | 162, 542, 578, 599, 1202, 1233, 1425, 1456, 1464 |
| NlaIV | GGNNCC | 4 cut(s) | 563, 753, 1179, 1254 |
| NmuCI | GTSAC | 1 cut(s) | 145 |
| NsiI | ATGCAT | 1 cut(s) | 1462 |
| NspI | RCATGY | 1 cut(s) | 1456 |
| NspV | TTCGAA | 1 cut(s) | 856 |
| PagI | TCATGA | 3 cut(s) | 538, 595, 1198 |
| PaqCI | CACCTGC | 1 cut(s) | 690 |
| PciI | ACATGT | 1 cut(s) | 1452 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 148, 1050 |
| PctI | GAATGC | 1 cut(s) | 1286 |
| PfeI | GAWTC | 4 cut(s) | 80, 90, 170, 181 |
| PflFI | GACNNNGTC | 1 cut(s) | 233 |
| PkrI | GCNGC | 3 cut(s) | 97, 420, 457 |
| Ple19I | CGATCG | 1 cut(s) | 1057 |
| PleI | GAGTC | 3 cut(s) | 182, 242, 1316 |
| PpsI | GAGTC | 3 cut(s) | 182, 242, 1316 |
| Ppu21I | YACGTR | 1 cut(s) | 616 |
| PpuMI | RGGWCCY | 1 cut(s) | 1252 |
| PscI | ACATGT | 1 cut(s) | 1452 |
| PshBI | ATTAAT | 1 cut(s) | 315 |
| PsiI | TTATAA | 1 cut(s) | 888 |
| Psp5II | RGGWCCY | 1 cut(s) | 1252 |
| Psp6I | CCWGG | 3 cut(s) | 745, 776, 1383 |
| PspFI | CCCAGC | 1 cut(s) | 423 |
| PspGI | CCWGG | 3 cut(s) | 745, 776, 1383 |
| PspN4I | GGNNCC | 4 cut(s) | 563, 753, 1179, 1254 |
| PspPI | GGNCC | 3 cut(s) | 21, 798, 1252 |
| PspPPI | RGGWCCY | 1 cut(s) | 1252 |
| PstNI | CAGNNNCTG | 2 cut(s) | 197, 1384 |
| PsuI | RGATCY | 3 cut(s) | 466, 670, 751 |
| PsyI | GACNNNGTC | 1 cut(s) | 233 |
| PvuI | CGATCG | 1 cut(s) | 1057 |
| RsaI | GTAC | 6 cut(s) | 579, 758, 1146, 1163, 1313, 1382 |
| RsaNI | GTAC | 6 cut(s) | 578, 757, 1145, 1162, 1312, 1381 |
| RseI | CAYNNNNRTG | 5 cut(s) | 130, 600, 968, 1112, 1203 |
| SaqAI | TTAA | 4 cut(s) | 315, 414, 588, 677 |
| SatI | GCNGC | 3 cut(s) | 96, 419, 456 |
| Sau3AI | GATC | 6 cut(s) | 406, 466, 670, 751, 1048, 1054 |
| Sau96I | GGNCC | 3 cut(s) | 21, 798, 1252 |
| SchI | GAGTC | 3 cut(s) | 182, 243, 1316 |
| ScrFI | CCNGG | 3 cut(s) | 747, 778, 1385 |
| SduI | GDGCHC | 3 cut(s) | 311, 564, 1411 |
| SfaNI | GCATC | 3 cut(s) | 37, 265, 1140 |
| SfcI | CTRYAG | 1 cut(s) | 214 |
| SfuI | TTCGAA | 1 cut(s) | 856 |
| SinI | GGWCC | 3 cut(s) | 21, 798, 1252 |
| SmiMI | CAYNNNNRTG | 5 cut(s) | 130, 600, 968, 1112, 1203 |
| SmlI | CTYRAG | 1 cut(s) | 245 |
| SmoI | CTYRAG | 1 cut(s) | 245 |
| SnaBI | TACGTA | 1 cut(s) | 616 |
| SpeI | ACTAGT | 1 cut(s) | 1067 |
| SsiI | CCGC | 3 cut(s) | 453, 768, 1029 |
| SspI | AATATT | 2 cut(s) | 355, 1081 |
| SspMI | CTAG | 4 cut(s) | 74, 185, 1068, 1104 |
| StyD4I | CCNGG | 3 cut(s) | 745, 776, 1383 |
| StyI | CCWWGG | 2 cut(s) | 158, 551 |
| TaaI | ACNGT | 2 cut(s) | 199, 218 |
| TaiI | ACGT | 3 cut(s) | 301, 618, 638 |
| TaqI | TCGA | 6 cut(s) | 78, 173, 509, 856, 980, 1044 |
| TaqII | GACCGA | 1 cut(s) | 446 |
| TatI | WGTACW | 2 cut(s) | 577, 1144 |
| TfiI | GAWTC | 4 cut(s) | 80, 90, 170, 181 |
| Tru1I | TTAA | 4 cut(s) | 315, 414, 588, 677 |
| Tru9I | TTAA | 4 cut(s) | 315, 414, 588, 677 |
| TseFI | GTSAC | 1 cut(s) | 145 |
| TseI | GCWGC | 3 cut(s) | 95, 418, 455 |
| Tsp45I | GTSAC | 1 cut(s) | 145 |
| TspGWI | ACGGA | 1 cut(s) | 744 |
| Tth111I | GACNNNGTC | 1 cut(s) | 233 |
| VpaK11BI | GGWCC | 3 cut(s) | 21, 798, 1252 |
| VspI | ATTAAT | 1 cut(s) | 315 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 555 |
| XapI | RAATTY | 5 cut(s) | 434, 511, 590, 1033, 1512 |
| XbaI | TCTAGA | 1 cut(s) | 184 |
| XceI | RCATGY | 1 cut(s) | 1456 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 792 |
| XmiI | GTMKAC | 2 cut(s) | 237, 643 |
| XspI | CTAG | 4 cut(s) | 74, 185, 1068, 1104 |
| Zsp2I | ATGCAT | 1 cut(s) | 1462 |