Protein of unknown function (DUF295)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 154 |
| AclWI | GGATC | 3 cut(s) | 116, 122, 305 |
| AcsI | RAATTY | 3 cut(s) | 8, 40, 257 |
| AfaI | GTAC | 1 cut(s) | 296 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 62, 105 |
| AjnI | CCWGG | 2 cut(s) | 114, 261 |
| AluBI | AGCT | 1 cut(s) | 274 |
| AluI | AGCT | 1 cut(s) | 274 |
| Alw26I | GTCTC | 1 cut(s) | 39 |
| AlwI | GGATC | 3 cut(s) | 116, 122, 305 |
| AoxI | GGCC | 2 cut(s) | 117, 194 |
| ApeKI | GCWGC | 1 cut(s) | 79 |
| ApoI | RAATTY | 3 cut(s) | 8, 40, 257 |
| ArsI | GACNNNNNNTTYG | 1 cut(s) | 27 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 44 |
| AspLEI | GCGC | 1 cut(s) | 98 |
| AsuHPI | GGTGA | 1 cut(s) | 103 |
| BanI | GGYRCC | 1 cut(s) | 154 |
| BbvI | GCAGC | 1 cut(s) | 66 |
| BccI | CCATC | 1 cut(s) | 260 |
| BciT130I | CCWGG | 2 cut(s) | 116, 263 |
| BclI | TGATCA | 1 cut(s) | 328 |
| BcoDI | GTCTC | 1 cut(s) | 39 |
| BfmI | CTRYAG | 1 cut(s) | 123 |
| BfoI | RGCGCY | 1 cut(s) | 99 |
| BisI | GCNGC | 1 cut(s) | 80 |
| BlsI | GCNGC | 1 cut(s) | 81 |
| Bme1390I | CCNGG | 2 cut(s) | 116, 263 |
| BmiI | GGNNCC | 1 cut(s) | 156 |
| BmrFI | CCNGG | 2 cut(s) | 116, 263 |
| BsaAI | YACGTR | 1 cut(s) | 298 |
| BsaI | GGTCTC | 1 cut(s) | 39 |
| BsaJI | CCNNGG | 2 cut(s) | 104, 114 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 62, 105 |
| Bse1I | ACTGG | 1 cut(s) | 93 |
| Bse3DI | GCAATG | 1 cut(s) | 288 |
| BseBI | CCWGG | 2 cut(s) | 116, 263 |
| BseDI | CCNNGG | 2 cut(s) | 104, 114 |
| BseGI | GGATG | 1 cut(s) | 271 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 62, 105 |
| BseMI | GCAATG | 1 cut(s) | 288 |
| BseMII | CTCAG | 1 cut(s) | 50 |
| BseNI | ACTGG | 1 cut(s) | 93 |
| BseXI | GCAGC | 1 cut(s) | 66 |
| BshFI | GGCC | 2 cut(s) | 119, 196 |
| BshNI | GGYRCC | 1 cut(s) | 154 |
| BslFI | GGGAC | 1 cut(s) | 300 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 62, 105 |
| BsmAI | GTCTC | 1 cut(s) | 39 |
| BsmFI | GGGAC | 1 cut(s) | 300 |
| BsnI | GGCC | 2 cut(s) | 119, 196 |
| Bso31I | GGTCTC | 1 cut(s) | 39 |
| Bsp143I | GATC | 4 cut(s) | 108, 127, 310, 328 |
| Bsp19I | CCATGG | 1 cut(s) | 104 |
| BspANI | GGCC | 2 cut(s) | 119, 196 |
| BspCNI | CTCAG | 1 cut(s) | 49 |
| BspLI | GGNNCC | 1 cut(s) | 156 |
| BspMAI | CTGCAG | 1 cut(s) | 127 |
| BspPI | GGATC | 3 cut(s) | 116, 122, 305 |
| BspT107I | GGYRCC | 1 cut(s) | 154 |
| BspTNI | GGTCTC | 1 cut(s) | 39 |
| BsrDI | GCAATG | 1 cut(s) | 288 |
| BsrI | ACTGG | 1 cut(s) | 93 |
| BssECI | CCNNGG | 2 cut(s) | 104, 114 |
| BssMI | GATC | 4 cut(s) | 108, 127, 310, 328 |
| BssT1I | CCWWGG | 1 cut(s) | 104 |
| Bst2UI | CCWGG | 2 cut(s) | 116, 263 |
| BstBAI | YACGTR | 1 cut(s) | 298 |
| BstC8I | GCNNGC | 2 cut(s) | 100, 194 |
| BstDEI | CTNAG | 2 cut(s) | 36, 316 |
| BstDSI | CCRYGG | 1 cut(s) | 104 |
| BstF5I | GGATG | 1 cut(s) | 271 |
| BstH2I | RGCGCY | 1 cut(s) | 99 |
| BstHHI | GCGC | 1 cut(s) | 98 |
| BstKTI | GATC | 4 cut(s) | 111, 130, 313, 331 |
| BstMAI | GTCTC | 1 cut(s) | 39 |
| BstMBI | GATC | 4 cut(s) | 108, 127, 310, 328 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 79 |
| BstNI | CCWGG | 2 cut(s) | 116, 263 |
| BstSCI | CCNGG | 2 cut(s) | 114, 261 |
| BstSFI | CTRYAG | 1 cut(s) | 123 |
| BstSNI | TACGTA | 1 cut(s) | 298 |
| BstV1I | GCAGC | 1 cut(s) | 66 |
| BstX2I | RGATCY | 2 cut(s) | 108, 127 |
| BstYI | RGATCY | 2 cut(s) | 108, 127 |
| BsuRI | GGCC | 2 cut(s) | 119, 196 |
| BtgI | CCRYGG | 1 cut(s) | 104 |
| BtsCI | GGATG | 1 cut(s) | 271 |
| BtsIMutI | CAGTG | 1 cut(s) | 86 |
| Cac8I | GCNNGC | 2 cut(s) | 100, 194 |
| CfoI | GCGC | 1 cut(s) | 98 |
| Csp6I | GTAC | 1 cut(s) | 295 |
| CviAII | CATG | 2 cut(s) | 105, 244 |
| CviJI | RGCY | 6 cut(s) | 68, 73, 119, 196, 248, 274 |
| CviKI_1 | RGCY | 6 cut(s) | 68, 73, 119, 196, 248, 274 |
| CviQI | GTAC | 1 cut(s) | 295 |
| DdeI | CTNAG | 2 cut(s) | 36, 316 |
| DpnI | GATC | 4 cut(s) | 110, 129, 312, 330 |
| DpnII | GATC | 4 cut(s) | 108, 127, 310, 328 |
| Eco105I | TACGTA | 1 cut(s) | 298 |
| Eco130I | CCWWGG | 1 cut(s) | 104 |
| Eco31I | GGTCTC | 1 cut(s) | 39 |
| EcoRII | CCWGG | 2 cut(s) | 114, 261 |
| EcoT14I | CCWWGG | 1 cut(s) | 104 |
| EcoT22I | ATGCAT | 1 cut(s) | 149 |
| ErhI | CCWWGG | 1 cut(s) | 104 |
| FaeI | CATG | 2 cut(s) | 108, 247 |
| FaiI | YATR | 6 cut(s) | 77, 106, 166, 168, 245, 277 |
| FaqI | GGGAC | 1 cut(s) | 300 |
| FatI | CATG | 2 cut(s) | 104, 243 |
| FbaI | TGATCA | 1 cut(s) | 328 |
| Fnu4HI | GCNGC | 1 cut(s) | 80 |
| FokI | GGATG | 1 cut(s) | 278 |
| Fsp4HI | GCNGC | 1 cut(s) | 80 |
| GlaI | GCGC | 1 cut(s) | 97 |
| GluI | GCNGC | 1 cut(s) | 80 |
| HaeII | RGCGCY | 1 cut(s) | 99 |
| HaeIII | GGCC | 2 cut(s) | 119, 196 |
| HhaI | GCGC | 1 cut(s) | 98 |
| Hin1II | CATG | 2 cut(s) | 108, 247 |
| Hin6I | GCGC | 1 cut(s) | 96 |
| HinP1I | GCGC | 1 cut(s) | 96 |
| HinfI | GANTC | 2 cut(s) | 206, 214 |
| HphI | GGTGA | 1 cut(s) | 103 |
| Hpy166II | GTNNAC | 1 cut(s) | 213 |
| Hpy188I | TCNGA | 2 cut(s) | 39, 310 |
| Hpy188III | TCNNGA | 1 cut(s) | 326 |
| Hpy8I | GTNNAC | 1 cut(s) | 213 |
| HpyCH4IV | ACGT | 2 cut(s) | 22, 297 |
| HpyCH4V | TGCA | 3 cut(s) | 125, 147, 192 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 79 |
| HpyF3I | CTNAG | 2 cut(s) | 36, 316 |
| HpySE526I | ACGT | 2 cut(s) | 22, 297 |
| Hsp92II | CATG | 2 cut(s) | 108, 247 |
| HspAI | GCGC | 1 cut(s) | 96 |
| Ksp22I | TGATCA | 1 cut(s) | 328 |
| Kzo9I | GATC | 4 cut(s) | 108, 127, 310, 328 |
| LpnPI | CCDG | 9 cut(s) | 48, 74, 101, 112, 128, 178, 213, 248, 275 |
| Lsp1109I | GCAGC | 1 cut(s) | 66 |
| MaeII | ACGT | 2 cut(s) | 22, 297 |
| MalI | GATC | 4 cut(s) | 110, 129, 312, 330 |
| MboI | GATC | 4 cut(s) | 108, 127, 310, 328 |
| MflI | RGATCY | 2 cut(s) | 108, 127 |
| MluCI | AATT | 6 cut(s) | 8, 28, 40, 159, 252, 257 |
| MlyI | GAGTC | 1 cut(s) | 208 |
| MmeI | TCCRAC | 1 cut(s) | 244 |
| MnlI | CCTC | 1 cut(s) | 130 |
| Mph1103I | ATGCAT | 1 cut(s) | 149 |
| MroXI | GAANNNNTTC | 1 cut(s) | 44 |
| MseI | TTAA | 2 cut(s) | 228, 255 |
| MspR9I | CCNGG | 2 cut(s) | 116, 263 |
| MvaI | CCWGG | 2 cut(s) | 116, 263 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 79 |
| NcoI | CCATGG | 1 cut(s) | 104 |
| NdeII | GATC | 4 cut(s) | 108, 127, 310, 328 |
| NlaIII | CATG | 2 cut(s) | 108, 247 |
| NlaIV | GGNNCC | 1 cut(s) | 156 |
| NsiI | ATGCAT | 1 cut(s) | 149 |
| PdmI | GAANNNNTTC | 1 cut(s) | 44 |
| PfeI | GAWTC | 1 cut(s) | 206 |
| PfoI | TCCNGGA | 1 cut(s) | 261 |
| PkrI | GCNGC | 1 cut(s) | 81 |
| PleI | GAGTC | 1 cut(s) | 208 |
| PpsI | GAGTC | 1 cut(s) | 208 |
| Ppu21I | YACGTR | 1 cut(s) | 298 |
| Psp6I | CCWGG | 2 cut(s) | 114, 261 |
| PspGI | CCWGG | 2 cut(s) | 114, 261 |
| PspN4I | GGNNCC | 1 cut(s) | 156 |
| PstI | CTGCAG | 1 cut(s) | 127 |
| PsuI | RGATCY | 2 cut(s) | 108, 127 |
| RsaI | GTAC | 1 cut(s) | 296 |
| RsaNI | GTAC | 1 cut(s) | 295 |
| SaqAI | TTAA | 2 cut(s) | 228, 255 |
| SatI | GCNGC | 1 cut(s) | 80 |
| Sau3AI | GATC | 4 cut(s) | 108, 127, 310, 328 |
| SchI | GAGTC | 1 cut(s) | 208 |
| ScrFI | CCNGG | 2 cut(s) | 116, 263 |
| SetI | ASST | 5 cut(s) | 25, 182, 202, 276, 300 |
| SfcI | CTRYAG | 1 cut(s) | 123 |
| SnaBI | TACGTA | 1 cut(s) | 298 |
| Sse9I | AATT | 6 cut(s) | 8, 28, 40, 159, 252, 257 |
| StyD4I | CCNGG | 2 cut(s) | 114, 261 |
| StyI | CCWWGG | 1 cut(s) | 104 |
| TaiI | ACGT | 2 cut(s) | 25, 300 |
| TasI | AATT | 6 cut(s) | 8, 28, 40, 159, 252, 257 |
| TfiI | GAWTC | 1 cut(s) | 206 |
| Tru1I | TTAA | 2 cut(s) | 228, 255 |
| Tru9I | TTAA | 2 cut(s) | 228, 255 |
| TscAI | CASTG | 1 cut(s) | 93 |
| TseI | GCWGC | 1 cut(s) | 79 |
| TspDTI | ATGAA | 4 cut(s) | 32, 37, 219, 252 |
| TspRI | CASTG | 1 cut(s) | 93 |
| XapI | RAATTY | 3 cut(s) | 8, 40, 257 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 148 |
| XmnI | GAANNNNTTC | 1 cut(s) | 44 |
| Zsp2I | ATGCAT | 1 cut(s) | 149 |