AT3G59360

transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0008649
Physical Location & Seq
Reverse (-)
21935278 .. 21940365
5088 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G59360.1

Sequence Viewer

Length: 1218 bp
ATGAAGAACGGTATAGCTGAGTGCCCTGCATGTCATTCAAAATTGGTCTCTCCTGGTAGCAAAACTATTTCTAGGGCTTATGACGACCACAAGATTAGGGTATCCTCAAAACAACGTGTCCTCAATGTCCTTTTGGTTGTAGGTGATTGCATGCTTGTTGGTCTCCAGCCTGTCTTGGTGTATATGTCGAAGGTGGATGGGAAATTCAACTTCAGTCCTATTAGCGTCAACTTCTTGACAGAAATCGCAAAAGTTATTTTTGCGATTGTGATGCTTTTAATCCAGGCCAGGCACCAAAAAGTTGGAGAGAAGCCTCTTCTATCCGTTTCCACATTTGTCCAGGCAGCCCGAAATAATGTGCTTCTTGCTGTTCCAGCCCTGTTGTATGCAATTAATAACTATCTGAAGTTCACAATGCAGCTATATTTCAATCCTGCTACTGTAAAGATGCTTAGCAACCTAAAGGTTCTGGTCATCGCTGTTCTATTAAAGATGGTAATGAAGCGGCGGTTTTCTATCATACAGTGGGAGGCACTTGCTCTGTTGCTGATAGGGATTAGTGTAAACCAATTACGTTCTCTTCCTGAAGGTGCTACAGCCATTGGAATTCCTCTTGCTACGGGTGCATACGTCTGCACCGTTATCTTTGTCACTGTCCCGTCAATGGCATCTGTCTTCAACGAGTATGCTCTAAAGAGTCAGTATGACACAAGCATATATCTTCAGAATTTATTCCTCTATGGTTATGGTGCAATATTCAACTTTCTTGGAATACTAGGAACTGTTATATATAAAGGTCCTGGCAGCTTTGACATCCTACAAGGACATTCTAGGGCTACCATGTTTTTGATACTGAACAACGCAGCACAAGGGATTTTATCGTCCTTTTTCTTCAAATATGCAGATACAATCTTGAAGAAATATTCATCGACAGTTGCCACAATATTTACTGGCATAGCATCTGCTGCGCTCTTTGGACATGTTATAACCATGAACTTCCTTCTCGGAATTTCTATCGTTTTCATTTCGATGCACCAGTTCTTTTCACCACTTGCCAAAGCGAGAGACGAGCAACAACAGAATGGGAACCTAGAACTAGGCAACACAAAGGATACTCACAGGGCTAATGAATCATTCATCAACATGGCCGCAGGAGCAAATGAAGAGGCTAGTCACCGCGGTGAATCAGACGACAGGACTCCACTGCTTCCCAGATGA

Protein Analysis

405

Amino Acids

44.54

Weight (kDa)

9.52

Isoelectric Point (pI)

35.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nuc_sug_transp PF04142 58 - 344 2.8e-32 Nucleotide-sugar transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 986
AccB1I GGYRCC 1 cut(s) 291
AccII CGCG 1 cut(s) 1179
AciI CCGC 5 cut(s) 505, 508, 1149, 1177, 1179
AcoI YGGCCR 1 cut(s) 1146
AcsI RAATTY 4 cut(s) 203, 606, 727, 1008
AcuI CTGAAG 4 cut(s) 196, 425, 606, 707
AfiI CCNNNNNNNGG 1 cut(s) 664
AflIII ACRYGT 2 cut(s) 115, 979
AgsI TTSAA 7 cut(s) 39, 208, 430, 679, 760, 895, 916
AjnI CCWGG 5 cut(s) 52, 282, 287, 339, 799
AleI CACNNNNGTG 1 cut(s) 1179
AluBI AGCT 3 cut(s) 17, 421, 807
AluI AGCT 3 cut(s) 17, 421, 807
Alw26I GTCTC 3 cut(s) 52, 167, 1059
AoxI GGCC 2 cut(s) 285, 1146
ApeKI GCWGC 5 cut(s) 344, 418, 804, 863, 965
ApoI RAATTY 4 cut(s) 203, 606, 727, 1008
AseI ATTAAT 1 cut(s) 393
Asp700I GAANNNNTTC 1 cut(s) 731
AspLEI GCGC 1 cut(s) 970
AspS9I GGNCC 1 cut(s) 797
AsuHPI GGTGA 4 cut(s) 155, 1038, 1166, 1193
AvaII GGWCC 1 cut(s) 797
BaeGI GKGCMC 1 cut(s) 26
BanI GGYRCC 1 cut(s) 291
BbsI GAAGAC 1 cut(s) 667
BbvI GCAGC 5 cut(s) 356, 430, 816, 875, 952
BccI CCATC 2 cut(s) 191, 487
BcgI CGANNNNNNTGC 2 cut(s) 253, 287
BciT130I CCWGG 5 cut(s) 54, 284, 289, 341, 801
BciVI GTATCC 2 cut(s) 112, 1105
BcoDI GTCTC 3 cut(s) 52, 167, 1059
BfaI CTAG 6 cut(s) 72, 776, 831, 1091, 1097, 1170
BfmI CTRYAG 1 cut(s) 594
BfuI GTATCC 2 cut(s) 112, 1105
BisI GCNGC 7 cut(s) 345, 419, 506, 805, 864, 966, 1149
BlpI GCTNAGC 1 cut(s) 452
BlsI GCNGC 7 cut(s) 346, 420, 507, 806, 865, 967, 1150
Bme1390I CCNGG 5 cut(s) 54, 284, 289, 341, 801
Bme18I GGWCC 1 cut(s) 797
BmgT120I GGNCC 1 cut(s) 797
BmiI GGNNCC 2 cut(s) 293, 1088
BmrFI CCNGG 5 cut(s) 54, 284, 289, 341, 801
BmsI GCATC 5 cut(s) 261, 438, 677, 968, 1020
BpiI GAAGAC 1 cut(s) 667
BpmI CTGGAG 1 cut(s) 149
Bpu1102I GCTNAGC 1 cut(s) 452
BsaI GGTCTC 2 cut(s) 52, 167
BsaJI CCNNGG 1 cut(s) 1177
Bsc4I CCNNNNNNNGG 1 cut(s) 664
Bse1I ACTGG 2 cut(s) 955, 1036
BseBI CCWGG 5 cut(s) 54, 284, 289, 341, 801
BseDI CCNNGG 1 cut(s) 1177
BseGI GGATG 2 cut(s) 202, 813
BseLI CCNNNNNNNGG 1 cut(s) 664
BseMII CTCAG 1 cut(s) 9
BseNI ACTGG 2 cut(s) 955, 1036
BseSI GKGCMC 1 cut(s) 26
BseXI GCAGC 5 cut(s) 356, 430, 816, 875, 952
BsgI GTGCAG 1 cut(s) 619
Bsh1236I CGCG 1 cut(s) 1179
BshFI GGCC 2 cut(s) 287, 1148
BshNI GGYRCC 1 cut(s) 291
BslFI GGGAC 1 cut(s) 641
BslI CCNNNNNNNGG 1 cut(s) 664
BsmAI GTCTC 3 cut(s) 52, 167, 1059
BsmBI CGTCTC 1 cut(s) 1059
BsmFI GGGAC 1 cut(s) 641
BsnI GGCC 2 cut(s) 287, 1148
Bso31I GGTCTC 2 cut(s) 52, 167
Bsp1286I GDGCHC 1 cut(s) 26
Bsp1720I GCTNAGC 1 cut(s) 452
BspACI CCGC 5 cut(s) 505, 508, 1149, 1177, 1179
BspANI GGCC 2 cut(s) 287, 1148
BspCNI CTCAG 1 cut(s) 10
BspFNI CGCG 1 cut(s) 1179
BspLI GGNNCC 2 cut(s) 293, 1088
BspT107I GGYRCC 1 cut(s) 291
BspTNI GGTCTC 2 cut(s) 52, 167
BsrI ACTGG 2 cut(s) 955, 1036
BssECI CCNNGG 1 cut(s) 1177
Bst2UI CCWGG 5 cut(s) 54, 284, 289, 341, 801
Bst4CI ACNGT 7 cut(s) 11, 442, 525, 640, 655, 784, 934
Bst6I CTCTTC 3 cut(s) 321, 585, 1158
BstAPI GCANNNNNTGC 1 cut(s) 965
BstC8I GCNNGC 1 cut(s) 152
BstDEI CTNAG 2 cut(s) 18, 452
BstDSI CCRYGG 1 cut(s) 1177
BstF5I GGATG 2 cut(s) 202, 813
BstFNI CGCG 1 cut(s) 1179
BstHHI GCGC 1 cut(s) 970
BstMAI GTCTC 3 cut(s) 52, 167, 1059
BstMWI GCNNNNNNNGC 4 cut(s) 374, 623, 965, 1154
BstNI CCWGG 5 cut(s) 54, 284, 289, 341, 801
BstNSI RCATGY 3 cut(s) 33, 154, 983
BstSCI CCNGG 5 cut(s) 52, 282, 287, 339, 799
BstSFI CTRYAG 1 cut(s) 594
BstSLI GKGCMC 1 cut(s) 26
BstUI CGCG 1 cut(s) 1179
BstV1I GCAGC 5 cut(s) 356, 430, 816, 875, 952
BstV2I GAAGAC 1 cut(s) 667
BstXI CCANNNNNNTGG 1 cut(s) 302
BsuI GTATCC 2 cut(s) 112, 1105
BsuRI GGCC 2 cut(s) 287, 1148
BtgI CCRYGG 1 cut(s) 1177
BtgZI GCGATG 1 cut(s) 460
BtsCI GGATG 2 cut(s) 202, 813
BtsI GCAGTG 1 cut(s) 1202
BtsIMutI CAGTG 3 cut(s) 530, 651, 1202
Cac8I GCNNGC 1 cut(s) 152
CfoI GCGC 1 cut(s) 970
Cfr13I GGNCC 1 cut(s) 797
Cfr42I CCGCGG 1 cut(s) 1180
CseI GACGC 1 cut(s) 214
CviAII CATG 6 cut(s) 30, 151, 841, 980, 991, 1144
DdeI CTNAG 2 cut(s) 18, 452
EaeI YGGCCR 1 cut(s) 1146
Eam1104I CTCTTC 3 cut(s) 321, 585, 1158
EarI CTCTTC 3 cut(s) 321, 585, 1158
Eco31I GGTCTC 2 cut(s) 52, 167
Eco47I GGWCC 1 cut(s) 797
Eco57I CTGAAG 4 cut(s) 196, 425, 606, 707
EcoO109I RGGNCCY 1 cut(s) 797
EcoRI GAATTC 1 cut(s) 606
EcoRII CCWGG 5 cut(s) 52, 282, 287, 339, 799
Esp3I CGTCTC 1 cut(s) 1059
FaeI CATG 6 cut(s) 33, 154, 844, 983, 994, 1147
FaqI GGGAC 1 cut(s) 641
FatI CATG 6 cut(s) 29, 150, 840, 979, 990, 1143
Fnu4HI GCNGC 7 cut(s) 345, 419, 506, 805, 864, 966, 1149
FokI GGATG 2 cut(s) 209, 800
Fsp4HI GCNGC 7 cut(s) 345, 419, 506, 805, 864, 966, 1149
FspBI CTAG 6 cut(s) 72, 776, 831, 1091, 1097, 1170
GlaI GCGC 1 cut(s) 969
GluI GCNGC 7 cut(s) 345, 419, 506, 805, 864, 966, 1149
GsuI CTGGAG 1 cut(s) 149
HaeIII GGCC 2 cut(s) 287, 1148
HgaI GACGC 1 cut(s) 214
HhaI GCGC 1 cut(s) 970
Hin1II CATG 6 cut(s) 33, 154, 844, 983, 994, 1147
Hin6I GCGC 1 cut(s) 968
HinP1I GCGC 1 cut(s) 968
HincII GTYRAC 1 cut(s) 229
HindII GTYRAC 1 cut(s) 229
HinfI GANTC 4 cut(s) 697, 1130, 1184, 1198
HphI GGTGA 4 cut(s) 155, 1038, 1166, 1193
Hpy166II GTNNAC 3 cut(s) 229, 411, 565
Hpy188I TCNGA 4 cut(s) 405, 726, 1007, 1189
Hpy188III TCNNGA 3 cut(s) 235, 584, 913
Hpy8I GTNNAC 3 cut(s) 229, 411, 565
HpyAV CCTTC 3 cut(s) 184, 581, 1010
HpyCH4III ACNGT 7 cut(s) 11, 442, 525, 640, 655, 784, 934
HpyCH4IV ACGT 3 cut(s) 115, 574, 630
HpyCH4V TGCA 9 cut(s) 29, 150, 389, 418, 626, 636, 752, 902, 1033
HpyF10VI GCNNNNNNNGC 4 cut(s) 374, 623, 965, 1154
HpyF3I CTNAG 2 cut(s) 18, 452
HpySE526I ACGT 3 cut(s) 115, 574, 630
Hsp92II CATG 6 cut(s) 33, 154, 844, 983, 994, 1147
HspAI GCGC 1 cut(s) 968
KspI CCGCGG 1 cut(s) 1180
LmnI GCTCC 1 cut(s) 1154
Lsp1109I GCAGC 5 cut(s) 356, 430, 816, 875, 952
LweI GCATC 5 cut(s) 261, 438, 677, 968, 1020
MaeI CTAG 6 cut(s) 72, 776, 831, 1091, 1097, 1170
MaeII ACGT 3 cut(s) 115, 574, 630
MaeIII GTNAC 2 cut(s) 649, 1172
MboII GAAGA 8 cut(s) 16, 308, 572, 667, 713, 883, 928, 1175
MhlI GDGCHC 1 cut(s) 26
MluCI AATT 7 cut(s) 41, 203, 390, 569, 606, 727, 1008
MlyI GAGTC 2 cut(s) 706, 1192
MmeI TCCRAC 1 cut(s) 283
MnlI CCTC 7 cut(s) 115, 131, 324, 523, 621, 746, 1159
MroXI GAANNNNTTC 1 cut(s) 731
MseI TTAA 3 cut(s) 278, 393, 488
MslI CAYNNNNRTG 3 cut(s) 1028, 1142, 1179
MspA1I CMGCKG 1 cut(s) 1179
MspR9I CCNGG 5 cut(s) 54, 284, 289, 341, 801
MvaI CCWGG 5 cut(s) 54, 284, 289, 341, 801
MvnI CGCG 1 cut(s) 1179
MwoI GCNNNNNNNGC 4 cut(s) 374, 623, 965, 1154
NlaIII CATG 6 cut(s) 33, 154, 844, 983, 994, 1147
NlaIV GGNNCC 2 cut(s) 293, 1088
NmuCI GTSAC 2 cut(s) 649, 1172
NspI RCATGY 3 cut(s) 33, 154, 983
OliI CACNNNNGTG 1 cut(s) 1179
PaeI GCATGC 1 cut(s) 154
PciI ACATGT 1 cut(s) 979
PcsI WCGNNNNNNNCGW 1 cut(s) 636
PdmI GAANNNNTTC 1 cut(s) 731
PfeI GAWTC 2 cut(s) 1130, 1184
PkrI GCNGC 7 cut(s) 346, 420, 507, 806, 865, 967, 1150
PleI GAGTC 2 cut(s) 705, 1192
PpsI GAGTC 2 cut(s) 705, 1192
PpuMI RGGWCCY 1 cut(s) 797
PscI ACATGT 1 cut(s) 979
PshBI ATTAAT 1 cut(s) 393
PsiI TTATAA 1 cut(s) 986
Psp5II RGGWCCY 1 cut(s) 797
Psp6I CCWGG 5 cut(s) 52, 282, 287, 339, 799
PspGI CCWGG 5 cut(s) 52, 282, 287, 339, 799
PspN4I GGNNCC 2 cut(s) 293, 1088
PspPI GGNCC 1 cut(s) 797
PspPPI RGGWCCY 1 cut(s) 797
RseI CAYNNNNRTG 3 cut(s) 1028, 1142, 1179
SacII CCGCGG 1 cut(s) 1180
SaqAI TTAA 3 cut(s) 278, 393, 488
SatI GCNGC 7 cut(s) 345, 419, 506, 805, 864, 966, 1149
Sau96I GGNCC 1 cut(s) 797
SchI GAGTC 2 cut(s) 706, 1192
ScrFI CCNGG 5 cut(s) 54, 284, 289, 341, 801
SduI GDGCHC 1 cut(s) 26
SfaNI GCATC 5 cut(s) 261, 438, 677, 968, 1020
SfcI CTRYAG 1 cut(s) 594
Sfr303I CCGCGG 1 cut(s) 1180
SgrBI CCGCGG 1 cut(s) 1180
SinI GGWCC 1 cut(s) 797
SmiMI CAYNNNNRTG 3 cut(s) 1028, 1142, 1179
SphI GCATGC 1 cut(s) 154
Sse9I AATT 7 cut(s) 41, 203, 390, 569, 606, 727, 1008
SsiI CCGC 5 cut(s) 505, 508, 1149, 1177, 1179
SspI AATATT 3 cut(s) 756, 923, 945
SspMI CTAG 6 cut(s) 72, 776, 831, 1091, 1097, 1170
StyD4I CCNGG 5 cut(s) 52, 282, 287, 339, 799
TaaI ACNGT 7 cut(s) 11, 442, 525, 640, 655, 784, 934
TaiI ACGT 3 cut(s) 118, 577, 633
TaqI TCGA 3 cut(s) 188, 929, 1028
TasI AATT 7 cut(s) 41, 203, 390, 569, 606, 727, 1008
TauI GCSGC 2 cut(s) 508, 1151
TfiI GAWTC 2 cut(s) 1130, 1184
Tru1I TTAA 3 cut(s) 278, 393, 488
Tru9I TTAA 3 cut(s) 278, 393, 488
TscAI CASTG 3 cut(s) 530, 658, 1209
TseFI GTSAC 2 cut(s) 649, 1172
TseI GCWGC 5 cut(s) 344, 418, 804, 863, 965
Tsp45I GTSAC 2 cut(s) 649, 1172
TspDTI ATGAA 8 cut(s) 17, 515, 915, 1007, 1012, 1126, 1143, 1176
TspGWI ACGGA 1 cut(s) 313
TspRI CASTG 3 cut(s) 530, 658, 1209
VpaK11BI GGWCC 1 cut(s) 797
VspI ATTAAT 1 cut(s) 393
XapI RAATTY 4 cut(s) 203, 606, 727, 1008
XceI RCATGY 3 cut(s) 33, 154, 983
XmnI GAANNNNTTC 1 cut(s) 731
XspI CTAG 6 cut(s) 72, 776, 831, 1091, 1097, 1170
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.